A Blesch

Summary

Affiliation: University of California
Country: USA

Publications

  1. ncbi Lentiviral and MLV based retroviral vectors for ex vivo and in vivo gene transfer
    Armin Blesch
    Department of Neurosciences, University of California, San Diego, La Jolla, CA 92093 0626, USA
    Methods 33:164-72. 2004
  2. ncbi Gene therapy and cell transplantation for Alzheimer's disease and spinal cord injury
    Armin Blesch
    Department of Neurosciences Center for Neural Repair, University of California, San Diego, La Jolla, CA 92093 0626, USA
    Yonsei Med J 45:28-31. 2004
  3. ncbi Cellular GDNF delivery promotes growth of motor and dorsal column sensory axons after partial and complete spinal cord transections and induces remyelination
    Armin Blesch
    Department of Neurosciences 0626, University of California San Diego, La Jolla, California 92093 0626, USA
    J Comp Neurol 467:403-17. 2003
  4. ncbi Axonal responses to cellularly delivered NT-4/5 after spinal cord injury
    Armin Blesch
    Department of Neurosciences, University of California, San Diego, La Jolla, CA 92093, USA
    Mol Cell Neurosci 27:190-201. 2004
  5. ncbi Regulated lentiviral NGF gene transfer controls rescue of medial septal cholinergic neurons
    Armin Blesch
    Department of Neurosciences 0626, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
    Mol Ther 11:916-25. 2005
  6. ncbi Neurotrophic factors in neurodegeneration
    Armin Blesch
    Department of Neurosciences 0626, Center for Neural Repair, University of California, San Diego, La Jolla, California 92093 0626, USA
    Brain Pathol 16:295-303. 2006
  7. ncbi Transient growth factor delivery sustains regenerated axons after spinal cord injury
    Armin Blesch
    Department of Neurosciences, University of California, San Diego, La Jolla, California 92093, USA
    J Neurosci 27:10535-45. 2007
  8. ncbi Neurotrophic factors, gene therapy, and neural stem cells for spinal cord repair
    Armin Blesch
    Department of Neurosciences, University of California, San Diego, La Jolla, CA 92093 0626, USA
    Brain Res Bull 57:833-8. 2002
  9. ncbi GDNF gene delivery to injured adult CNS motor neurons promotes axonal growth, expression of the trophic neuropeptide CGRP, and cellular protection
    A Blesch
    Department of Neurosciences 0626, University of California, San Diego, La Jolla, California 92093, USA
    J Comp Neurol 436:399-410. 2001
  10. ncbi Neurotrophism without neurotropism: BDNF promotes survival but not growth of lesioned corticospinal neurons
    P Lu
    Department of Neurosciences, University of California, San Diego, La Jolla, California 92093-0626, USA
    J Comp Neurol 436:456-70. 2001

Research Grants

Collaborators

Detail Information

Publications40

  1. ncbi Lentiviral and MLV based retroviral vectors for ex vivo and in vivo gene transfer
    Armin Blesch
    Department of Neurosciences, University of California, San Diego, La Jolla, CA 92093 0626, USA
    Methods 33:164-72. 2004
    ..This review will briefly summarize the background of these vector systems and provide some common protocols available for the preparation of MLV based retroviral vectors and HIV-1 based lentiviral vectors...
  2. ncbi Gene therapy and cell transplantation for Alzheimer's disease and spinal cord injury
    Armin Blesch
    Department of Neurosciences Center for Neural Repair, University of California, San Diego, La Jolla, CA 92093 0626, USA
    Yonsei Med J 45:28-31. 2004
    ..Thus, strategies have evolved for the delivery of potentially neuroprotective molecules, such as neurotrophic factors, and the replacement of cells and tissue lost due to CNS injury and degeneration...
  3. ncbi Cellular GDNF delivery promotes growth of motor and dorsal column sensory axons after partial and complete spinal cord transections and induces remyelination
    Armin Blesch
    Department of Neurosciences 0626, University of California San Diego, La Jolla, California 92093 0626, USA
    J Comp Neurol 467:403-17. 2003
    ..Thus, GDNF exerts tropic effects on adult spinal axons and Schwann cells that contribute to axon growth after injury...
  4. ncbi Axonal responses to cellularly delivered NT-4/5 after spinal cord injury
    Armin Blesch
    Department of Neurosciences, University of California, San Diego, La Jolla, CA 92093, USA
    Mol Cell Neurosci 27:190-201. 2004
    ..Thus, NT-4/5 and BDNF appear to be interchangeable to elicit substantial axonal growth in the injured spinal cord...
  5. ncbi Regulated lentiviral NGF gene transfer controls rescue of medial septal cholinergic neurons
    Armin Blesch
    Department of Neurosciences 0626, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
    Mol Ther 11:916-25. 2005
    ..These data demonstrate for the first time that NGF delivery by lentiviral gene transfer using tetracycline-regulated promoters can completely regulate neuronal rescue and protein production in the brain...
  6. ncbi Neurotrophic factors in neurodegeneration
    Armin Blesch
    Department of Neurosciences 0626, Center for Neural Repair, University of California, San Diego, La Jolla, California 92093 0626, USA
    Brain Pathol 16:295-303. 2006
    ..In this review, we will discuss some of the potential therapeutic applications of NTFs in neurodegenerative diseases and the potential contribution of disturbed neurotrophic factor signaling to neurodegenerative diseases...
  7. ncbi Transient growth factor delivery sustains regenerated axons after spinal cord injury
    Armin Blesch
    Department of Neurosciences, University of California, San Diego, La Jolla, California 92093, USA
    J Neurosci 27:10535-45. 2007
    ..Thus, the adult CNS appears capable of sustaining axons that have extended after transient growth factor delivery, an effect potentially attributable to the persistence of Schwann cells in lesion/graft sites...
  8. ncbi Neurotrophic factors, gene therapy, and neural stem cells for spinal cord repair
    Armin Blesch
    Department of Neurosciences, University of California, San Diego, La Jolla, CA 92093 0626, USA
    Brain Res Bull 57:833-8. 2002
    ..In this review we discuss the use of neural stem cell transplants and neurotrophic factor delivery by gene therapy to improve axonal regeneration in animal models of spinal cord injury...
  9. ncbi GDNF gene delivery to injured adult CNS motor neurons promotes axonal growth, expression of the trophic neuropeptide CGRP, and cellular protection
    A Blesch
    Department of Neurosciences 0626, University of California, San Diego, La Jolla, California 92093, USA
    J Comp Neurol 436:399-410. 2001
    ..Taken together with previous in vitro studies, these findings serve as the foundation for a model wherein GDNF and CGRP interact in a paracrine manner to regulate neuromuscular development and regeneration...
  10. ncbi Neurotrophism without neurotropism: BDNF promotes survival but not growth of lesioned corticospinal neurons
    P Lu
    Department of Neurosciences, University of California, San Diego, La Jolla, California 92093-0626, USA
    J Comp Neurol 436:456-70. 2001
    ..The possibility of tropic/trophic divergence must be considered when designing strategies to promote CNS recovery from injury...
  11. ncbi Modulation of neuronal survival and axonal growth in vivo by tetracycline-regulated neurotrophin expression
    A Blesch
    Department of Neurosciences-0626, University of California, San Diego, La Jolla, CA 92093-0626, USA
    Gene Ther 8:954-60. 2001
    ..These data constitute the first report of regulated neuronal rescue and axonal growth by controlled neurotrophin gene delivery and long-term, regulated expression using ex vivo CNS gene therapy...
  12. ncbi Nerve growth factor-hypersecreting Schwann cell grafts augment and guide spinal cord axonal growth and remyelinate central nervous system axons in a phenotypically appropriate manner that correlates with expression of L1
    N Weidner
    Department of Neurosciences, University of California San Diego, La Jolla, California 92093 0626, USA
    J Comp Neurol 413:495-506. 1999
    ....
  13. pmc IGF-I gene delivery promotes corticospinal neuronal survival but not regeneration after adult CNS injury
    Edmund R Hollis
    Department of Neurosciences, University of California, San Diego, La Jolla, CA 92093 0626, USA
    Exp Neurol 215:53-9. 2009
    ..We conclude that developmental patterns of growth factor responsiveness are not simply recapitulated after adult injury, potentially due to post-natal shifts in patterns of IGF-I receptor expression...
  14. pmc Local and remote growth factor effects after primate spinal cord injury
    John H Brock
    Department of Neurosciences, University of California, San Diego, La Jolla, California 92093, USA
    J Neurosci 30:9728-37. 2010
    ..Remote cortical effects of spinally administered growth factors could "prime" the neuron to respond to experimental therapies that promote axonal plasticity or regeneration...
  15. doi A dual promoter lentiviral vector for the in vivo evaluation of gene therapeutic approaches to axon regeneration after spinal cord injury
    K Low
    Department of Neurosciences, University of California San Diego, La Jolla, CA, USA
    Gene Ther 17:577-91. 2010
    ..This expression system is therefore an accurate and efficient means of screening candidate genes in vivo for enhancement of axonal growth...
  16. ncbi Neurotrophin-3 gradients established by lentiviral gene delivery promote short-distance axonal bridging beyond cellular grafts in the injured spinal cord
    Laura Taylor
    Department of Neurosciences, University of California, San Diego, La Jolla, California 92093, USA
    J Neurosci 26:9713-21. 2006
    ..These findings indicate that a localized and continuous gradient of NT-3 can achieve axonal bridging beyond the glial scar, but growth for longer distances is not sustainable simply with a trophic stimulus...
  17. pmc Chemotropic guidance facilitates axonal regeneration and synapse formation after spinal cord injury
    Laura Taylor Alto
    Department of Neurosciences, University of California, San Diego, La Jolla, California, USA
    Nat Neurosci 12:1106-13. 2009
    ..Thus, we report for the first time, to the best of our knowledge, the reinnervation of brainstem targets after SCI and an essential role for chemotropic axon guidance in target selection...
  18. pmc Combined intrinsic and extrinsic neuronal mechanisms facilitate bridging axonal regeneration one year after spinal cord injury
    Ken Kadoya
    Department of Neurosciences, University of California, San Diego, La Jolla, CA 92093, USA
    Neuron 64:165-72. 2009
    ..Collectively, these findings provide evidence that regeneration is achievable at unprecedented postinjury time points...
  19. pmc Long-term reversal of cholinergic neuronal decline in aged non-human primates by lentiviral NGF gene delivery
    Alan H Nagahara
    Department of Neurosciences 0626, University of California San Diego, La Jolla, California 92093, USA
    Exp Neurol 215:153-9. 2009
    ..These findings also support the safety and feasibility of lentiviral NGF gene transfer for potential testing in human clinical trials to protect degenerating cholinergic neurons in Alzheimer's disease...
  20. ncbi Spontaneous and augmented growth of axons in the primate spinal cord: effects of local injury and nerve growth factor-secreting cell grafts
    Mark H Tuszynski
    Department of Neurosciences, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0626, USA
    J Comp Neurol 449:88-101. 2002
    ..Furthermore, as in rodent studies, cellular delivery of a trophic factor significantly augments axonal plasticity in the primate spinal cord...
  21. ncbi Nerve growth factor gene therapy for Alzheimer's disease
    Mark H Tuszynski
    La Jolla, University of California San Diego, USA
    J Mol Neurosci 19:207. 2002
  22. ncbi New strategies in neural repair
    Mark H Tuszynski
    Department of Neurosciences 0626, University of California, San Diego, La Jolla, CA 92093 0626, USA
    Prog Brain Res 138:401-9. 2002
  23. pmc Induction of corticospinal regeneration by lentiviral trkB-induced Erk activation
    Edmund R Hollis
    Department of Neurosciences, University of California at San Diego, La Jolla, CA 92093 0626, USA
    Proc Natl Acad Sci U S A 106:7215-20. 2009
    ....
  24. ncbi A phase 1 clinical trial of nerve growth factor gene therapy for Alzheimer disease
    Mark H Tuszynski
    Department of Neurosciences, University of California at San Diego, La Jolla 92093, USA
    Nat Med 11:551-5. 2005
    ..05) increases in cortical 18-fluorodeoxyglucose after treatment. Brain autopsy from one subject suggested robust growth responses to NGF. Additional clinical trials of NGF for Alzheimer disease are warranted...
  25. ncbi NT-3 gene delivery elicits growth of chronically injured corticospinal axons and modestly improves functional deficits after chronic scar resection
    Mark H Tuszynski
    Department of Neurosciences, University of California, San Diego, La Jolla, CA 92093 0626, USA
    Exp Neurol 181:47-56. 2003
    ..Thus, growth factor gene delivery can elicit growth of corticospinal axons in chronic stages of injury and improves functional outcomes compared to non-growth-factor-treated animals...
  26. doi Regeneration of long-tract axons through sites of spinal cord injury using templated agarose scaffolds
    Thomas Gros
    Department of Bioengineering, University of California, San Diego, La Jolla, CA 92093, USA
    Biomaterials 31:6719-29. 2010
    ..Further development must reduce reactive cellular interfaces to support effective axonal penetration of host parenchyma...
  27. ncbi Nerve growth factor: from animal models of cholinergic neuronal degeneration to gene therapy in Alzheimer's disease
    Mark H Tuszynski
    Department of Neurosciences 0626, University of California, San Diego, La Jolla, CA 92093, USA
    Prog Brain Res 146:441-9. 2004
    ....
  28. pmc Neuroprotective effects of brain-derived neurotrophic factor in rodent and primate models of Alzheimer's disease
    Alan H Nagahara
    Department of Neurosciences 0626, 9500 Gilman Drive, University of California San Diego, La Jolla, California 92093, USA
    Nat Med 15:331-7. 2009
    ..BDNF therapeutic delivery merits exploration as a potential therapy for Alzheimer's disease...
  29. doi Spinal cord injury: plasticity, regeneration and the challenge of translational drug development
    Armin Blesch
    Department of Neurosciences 0626, University of California, San Diego, La Jolla, CA 92093, USA
    Trends Neurosci 32:41-7. 2009
    ..Therapeutic candidates are most likely to have a detectable effect in human trials if they elicit benefits in severe contusion and larger animal models and pass the test of independent replication...
  30. ncbi Spontaneous and neurotrophin-induced axonal plasticity after spinal cord injury
    Armin Blesch
    Department of Neurosciences 0626, University of California, 9500 Gilman Drive, San Diego, La Jolla, CA 92093 0626, USA
    Prog Brain Res 137:415-23. 2002
  31. ncbi A neurovascular niche for neurogenesis after stroke
    John J Ohab
    Department of Neurology, University of California, Los Angeles, Los Angeles, California 90095 1735, USA
    J Neurosci 26:13007-16. 2006
    ..These experiments define a novel brain environment for neuronal regeneration after stroke and identify molecular mechanisms that are shared between angiogenesis and neurogenesis during functional recovery from brain injury...
  32. ncbi Murine and HIV-based retroviral vectors for in vitro and in vivo gene transfer
    Ronald W Alfa
    Department of Neurosciences, University of California, San Diego, La Jolla, USA
    Methods Mol Med 129:241-54. 2006
    ..Here, we describe protocols to produce and standardize high quality MLV-based retroviral and HIV-based lentiviral vectors for ex vivo and in vivo gene delivery...
  33. ncbi Induction of bone marrow stromal cells to neurons: differentiation, transdifferentiation, or artifact?
    Paul Lu
    Department of Neurosciences, University of California, San Diego, La Jolla, California 92093, USA
    J Neurosci Res 77:174-91. 2004
    ....
  34. pmc A novel inducible tyrosine kinase receptor to regulate signal transduction and neurite outgrowth
    Ronald W Alfa
    Department of Neurosciences 0626, University of California, San Diego, La Jolla, California 92093 0626, USA
    J Neurosci Res 87:2624-31. 2009
    ..These results demonstrate that small ligand-induced dimerization of the intracellular domain of trkA can efficiently simulate the biological activity of NGF and provide a means to regulate intracellular neurotrophin receptor signaling...
  35. pmc Intranigral lentiviral delivery of dominant-negative TNF attenuates neurodegeneration and behavioral deficits in hemiparkinsonian rats
    Melissa K McCoy
    Department of Physiology, The University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    Mol Ther 16:1572-9. 2008
    ..This strategy holds the potential for therapeutic application in the treatment of PD...
  36. ncbi Loss of gene expression in lentivirus- and retrovirus-transduced neural progenitor cells is correlated to migration and differentiation in the adult spinal cord
    Maurice Vroemen
    Department of Neurology, University of Regensburg, 93053 Regensburg, Germany
    Exp Neurol 195:127-39. 2005
    ..Thus, in vivo gene expression in genetically engineered neural progenitor cells is temporally limited and mostly restricted to undifferentiated NPC using the viral vectors tested...
  37. ncbi Brain-derived neurotrophic factor gene transfer with adeno-associated viral and lentiviral vectors prevents rubrospinal neuronal atrophy and stimulates regeneration-associated gene expression after acute cervical spinal cord injury
    Brian K Kwon
    International Collaboration on Repair Discoveries, University of British Columbia, Vancouver, British Columbia, Canada
    Spine (Phila Pa 1976) 32:1164-73. 2007
    ..Experimental animal study...
  38. ncbi Adult neural progenitor cells provide a permissive guiding substrate for corticospinal axon growth following spinal cord injury
    Katharina Pfeifer
    Department of Neurology, University of Regensburg, Universitaetsstr 84, 93053 Regensburg, Germany
    Eur J Neurosci 20:1695-704. 2004
    ..Thus, grafted astroglial differentiated NPC promote axon regrowth following spinal cord injury by means of cellular guidance...
  39. ncbi Cloning and characterization of the expression pattern of a novel splice product MIA (splice) of malignant melanoma-derived growth-inhibiting activity (MIA/CD-RAP) [corrected]
    Peter Hau
    Department of Neurology, University of Regensburg, Regensburg, Germany
    J Invest Dermatol 119:562-9. 2002
    ..Whereas the biologic function of melanoma-inhibiting activity (splice) is not clear yet, it might provide a relevant diagnostic and therapeutic tool for malignant melanomas...
  40. ncbi Nucleus hears axon's pain
    Armin Blesch
    Nat Med 10:236-7. 2004

Research Grants7

  1. Regulatable Gene Therapy for Axonal Regeneration
    Armin Blesch; Fiscal Year: 2005
    ..Further, this could lead to the development of therapies for clinical translation. ..
  2. Regulatable Gene Transfer for Spinal Cord Injury
    Armin Blesch; Fiscal Year: 2010
    ..If successful, these studies will establish practical strategies for growth factor delivery to the injured spinal cord that can lead to the development of novel therapies for spinal cord injury. ..
  3. Regulatable Gene Transfer for Spinal Cord Injury
    Armin Blesch; Fiscal Year: 2009
    ..If successful, these studies will establish practical strategies for growth factor delivery to the injured spinal cord that can lead to the development of novel therapies for spinal cord injury. ..