Robert Blelloch

Summary

Affiliation: University of California
Country: USA

Publications

  1. pmc Genomic analysis suggests that mRNA destabilization by the microprocessor is specialized for the auto-regulation of Dgcr8
    Archana Shenoy
    The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, Center for Reproductive Sciences, and Department of Urology, University of California San Francisco, San Francisco, CA, USA
    PLoS ONE 4:e6971. 2009
  2. pmc Opposing microRNA families regulate self-renewal in mouse embryonic stem cells
    Collin Melton
    The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, Center for Reproductive Sciences, Program in Biomedical Sciences, University of California San Francisco, San Francisco, California 94143, USA
    Nature 463:621-6. 2010
  3. pmc microRNA induced transdifferentiation
    Archana Shenoy
    The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, Center for Reproductive Sciences Department of Urology, 35 Medical Center Way, Pod B 1018, University of California, San Francisco, San Francisco, CA 94143 0667 USA
    F1000 Biol Rep 4:3. 2012
  4. pmc Embryonic stem cell-specific microRNAs regulate the G1-S transition and promote rapid proliferation
    Yangming Wang
    Institute for Regeneration Medicine, Center for Reproductive Sciences and Department of Urology, University of California San Francisco, San Francisco, CA 94143, USA
    Nat Genet 40:1478-83. 2008
  5. ncbi request reprint microRNA control of mouse and human pluripotent stem cell behavior
    Tobias S Greve
    Department of Urology and The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, Center for Reproductive Sciences, Program in Biomedical Sciences, University of California, San Francisco, California, 94143 email
    Annu Rev Cell Dev Biol 29:213-39. 2013
  6. pmc MicroRNA function is globally suppressed in mouse oocytes and early embryos
    Nayoung Suh
    The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, San Francisco, CA 94143, USA
    Curr Biol 20:271-7. 2010
  7. pmc Mouse ES cells express endogenous shRNAs, siRNAs, and other Microprocessor-independent, Dicer-dependent small RNAs
    Joshua E Babiarz
    Institute for Regeneration Medicine, Center for Reproductive Sciences, University of California at San Francisco, San Francisco, California 94143, USA
    Genes Dev 22:2773-85. 2008
  8. pmc A role for noncanonical microRNAs in the mammalian brain revealed by phenotypic differences in Dgcr8 versus Dicer1 knockouts and small RNA sequencing
    Joshua E Babiarz
    The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, Center for Reproductive Sciences, University of California, San Francisco, San Francisco, California 94143, USA
    RNA 17:1489-501. 2011
  9. pmc Loss of microRNAs in pyramidal neurons leads to specific changes in inhibitory synaptic transmission in the prefrontal cortex
    Ruby Hsu
    Departments of Ophthalmology and Physiology, University of California, San Francisco, CA 94143, USA
    Mol Cell Neurosci 50:283-92. 2012
  10. pmc MicroRNA-29 regulates T-box transcription factors and interferon-γ production in helper T cells
    David F Steiner
    Sandler Asthma Basic Research Center and Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94143, USA
    Immunity 35:169-81. 2011

Collaborators

Detail Information

Publications31

  1. pmc Genomic analysis suggests that mRNA destabilization by the microprocessor is specialized for the auto-regulation of Dgcr8
    Archana Shenoy
    The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, Center for Reproductive Sciences, and Department of Urology, University of California San Francisco, San Francisco, CA, USA
    PLoS ONE 4:e6971. 2009
    ..The Microprocessor regulates its own levels by cleaving hairpins in the 5'UTR and coding region of the Dgcr8 mRNA, thereby destabilizing the mature transcript...
  2. pmc Opposing microRNA families regulate self-renewal in mouse embryonic stem cells
    Collin Melton
    The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, Center for Reproductive Sciences, Program in Biomedical Sciences, University of California San Francisco, San Francisco, California 94143, USA
    Nature 463:621-6. 2010
    ..Together, these findings show how the ESCC and let-7 miRNAs act through common pathways to alternatively stabilize the self-renewing versus differentiated cell fates...
  3. pmc microRNA induced transdifferentiation
    Archana Shenoy
    The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, Center for Reproductive Sciences Department of Urology, 35 Medical Center Way, Pod B 1018, University of California, San Francisco, San Francisco, CA 94143 0667 USA
    F1000 Biol Rep 4:3. 2012
    ..Here, we review this rapidly advancing field, discuss possible mechanisms underlying microRNA-induced transdifferentiation and the potential for microRNAs to drive such transitions to any cell type of interest in vitro and in vivo...
  4. pmc Embryonic stem cell-specific microRNAs regulate the G1-S transition and promote rapid proliferation
    Yangming Wang
    Institute for Regeneration Medicine, Center for Reproductive Sciences and Department of Urology, University of California San Francisco, San Francisco, CA 94143, USA
    Nat Genet 40:1478-83. 2008
    ..Our screening strategy provides an alternative and powerful approach for uncovering the role of individual miRNAs in biological processes, as it overcomes the common problem of redundancy and saturation in the miRNA system...
  5. ncbi request reprint microRNA control of mouse and human pluripotent stem cell behavior
    Tobias S Greve
    Department of Urology and The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, Center for Reproductive Sciences, Program in Biomedical Sciences, University of California, San Francisco, California, 94143 email
    Annu Rev Cell Dev Biol 29:213-39. 2013
    ..Here, we review the known mechanisms by which these and other microRNAs regulate the different aspects of the pluripotent stem cell program in both mouse and human. ..
  6. pmc MicroRNA function is globally suppressed in mouse oocytes and early embryos
    Nayoung Suh
    The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, San Francisco, CA 94143, USA
    Curr Biol 20:271-7. 2010
    ..These findings show that miRNA function is globally suppressed during oocyte maturation and preimplantation development and that endo-siRNAs, rather than miRNAs, underlie the Dicer knockout phenotype in oocytes...
  7. pmc Mouse ES cells express endogenous shRNAs, siRNAs, and other Microprocessor-independent, Dicer-dependent small RNAs
    Joshua E Babiarz
    Institute for Regeneration Medicine, Center for Reproductive Sciences, University of California at San Francisco, San Francisco, California 94143, USA
    Genes Dev 22:2773-85. 2008
    ..Our results extend the known diversity of mammalian small RNA-generating pathways and show that mammalian siRNAs exist in cell types other than oocytes...
  8. pmc A role for noncanonical microRNAs in the mammalian brain revealed by phenotypic differences in Dgcr8 versus Dicer1 knockouts and small RNA sequencing
    Joshua E Babiarz
    The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, Center for Reproductive Sciences, University of California, San Francisco, San Francisco, California 94143, USA
    RNA 17:1489-501. 2011
    ..Taken together, our findings provide evidence for a diverse population of highly expressed noncanonical miRNAs that together are likely to play important functional roles in post-mitotic neurons...
  9. pmc Loss of microRNAs in pyramidal neurons leads to specific changes in inhibitory synaptic transmission in the prefrontal cortex
    Ruby Hsu
    Departments of Ophthalmology and Physiology, University of California, San Francisco, CA 94143, USA
    Mol Cell Neurosci 50:283-92. 2012
    ....
  10. pmc MicroRNA-29 regulates T-box transcription factors and interferon-γ production in helper T cells
    David F Steiner
    Sandler Asthma Basic Research Center and Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94143, USA
    Immunity 35:169-81. 2011
    ..These results demonstrate that miR-29 regulates helper T cell differentiation by repressing multiple target genes, including at least two that are independently capable of inducing the T helper 1 (Th1) cell gene expression program...
  11. pmc DGCR8 is essential for microRNA biogenesis and silencing of embryonic stem cell self-renewal
    Yangming Wang
    Developmental and Stem Cell Biology Program, Department of Urology, University of California, San Francisco, California 94143, USA
    Nat Genet 39:380-5. 2007
    ..Our findings indicate that miRNAs function in the silencing of ES cell self-renewal that normally occurs with the induction of differentiation...
  12. pmc Distinct requirements of microRNAs in NK cell activation, survival, and function
    Natalie A Bezman
    Department of Microbiology and Immunology, Cancer Research Institute, University of California San Francisco, San Francisco, CA 94143, USA
    J Immunol 185:3835-46. 2010
    ..Thus, our findings indicate a critical role for miRNAs in controlling NK cell homeostasis and effector function, with implications for miRNAs regulating diverse aspects of NK cell biology...
  13. ncbi request reprint Regulation of epithelial-mesenchymal and mesenchymal-epithelial transitions by microRNAs
    Samy Lamouille
    Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, Department of Cell and Tissue Biology, University of California San Francisco, San Francisco, CA 94143, United States
    Curr Opin Cell Biol 25:200-7. 2013
    ..By affecting EMT and MET processes, microRNAs are involved in the regulation of stem cell pluripotency and the control of tumor progression...
  14. ncbi request reprint MicroRNA-based discovery of barriers to dedifferentiation of fibroblasts to pluripotent stem cells
    Robert L Judson
    1 The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, Center for Reproductive Sciences, Program in Biomedical Sciences, University of California San Francisco, San Francisco, California, USA 2 Department of Urology, University of California San Francisco, San Francisco, California, USA
    Nat Struct Mol Biol 20:1227-35. 2013
    ..These data establish two of the largest functionally defined networks of miRNA-mRNA interactions and reveal previously unidentified relationships among genes that act together to suppress early stages of reprogramming. ..
  15. pmc From microRNAs to targets: pathway discovery in cell fate transitions
    Deepa Subramanyam
    Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, Center for Reproductive Sciences, Department of Urology, University of California San Francisco, San Francisco, CA, USA
    Curr Opin Genet Dev 21:498-503. 2011
    ..Here, we review these findings and discuss how miRNAs may be used as tools to discover novel pathways that are involved in cell fate transitions using dedifferentiation of somatic cells to induced pluripotent stem cells as a case study...
  16. pmc Genome-wide analysis of translation reveals a critical role for deleted in azoospermia-like (Dazl) at the oocyte-to-zygote transition
    Jing Chen
    Center for Reproductive Sciences, University of California at San Francisco, San Francisco, California 94143, USA
    Genes Dev 25:755-66. 2011
    ..Therefore, Cpeb1 and Dazl function in a progressive, self-reinforcing pathway to promote oocyte maturation and early embryonic development...
  17. pmc Embryonic stem cell-specific microRNAs promote induced pluripotency
    Robert L Judson
    The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, San Francisco, California, USA
    Nat Biotechnol 27:459-61. 2009
    ..cMyc binds the promoter of the miRNAs, suggesting that they are downstream effectors of cMyc during reprogramming. However, unlike cMyc, the miRNAs induce a homogeneous population of iPS cell colonies...
  18. pmc Monoallelic deletion of the microRNA biogenesis gene Dgcr8 produces deficits in the development of excitatory synaptic transmission in the prefrontal cortex
    Claude M Schofield
    Department of Ophthalmology, University of California, San Francisco, San Francisco, CA 94143, USA
    Neural Dev 6:11. 2011
    ....
  19. pmc Microfluidic-based multiplex qRT-PCR identifies diagnostic and prognostic microRNA signatures in the sera of prostate cancer patients
    Felix Moltzahn
    The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, Department of Urology, UCSF Helen Diller Family Comprehensive Cancer Center, University of California San Francisco, San Francisco, California 94143, USA
    Cancer Res 71:550-60. 2011
    ..These serum signatures include oncogenic and tumor-suppressive miRNAs, suggesting functional roles in prostate cancer progression...
  20. pmc DGCR8-mediated production of canonical microRNAs is critical for regulatory T cell function and stability
    Lukas T Jeker
    Diabetes Center and the Department of Medicine, University of California San Francisco, San Francisco, California, United States of America
    PLoS ONE 8:e66282. 2013
    ..We conclude that canonical miRNAs are essential to maintain stable FoxP3 expression and Treg function. Thus, signals interfering with miRNA homeostasis might contribute to autoimmune diseases...
  21. pmc Generation of induced pluripotent stem cells from the prairie vole
    Devanand S Manoli
    Department of Anatomy, University of California San Francisco, San Francisco, California, United States of America
    PLoS ONE 7:e38119. 2012
    ....
  22. pmc CompMoby: comparative MobyDick for detection of cis-regulatory motifs
    Christina Chaivorapol
    Department of Biochemistry and Biophysics, California Institute for Quantitative Biomedical Research, Graduate Program in Biological and Medical Informatics, University of California, San Francisco, CA 94143 2540, USA
    BMC Bioinformatics 9:455. 2008
    ..Therefore, a computational algorithm should account for both conserved and nonconserved cis-regulatory elements in metazoans...
  23. pmc Sox1 marks an activated neural stem/progenitor cell in the hippocampus
    Monica Venere
    Department of Urology, University of California, San Francisco, CA 94143, USA
    Development 139:3938-49. 2012
    ..These results demonstrate that Sox1 is expressed in an activated stem/progenitor population whose numbers decrease with age while maintaining a stable molecular program...
  24. pmc Cell cycle regulation by MicroRNAs in embryonic stem cells
    Yangming Wang
    The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California San Francisco, San Francisco, CA 94143, USA
    Cancer Res 69:4093-6. 2009
    ..This review discusses these recent findings in the context of broader knowledge of cell cycle control in normal and abnormal development...
  25. pmc Multiple targets of miR-302 and miR-372 promote reprogramming of human fibroblasts to induced pluripotent stem cells
    Deepa Subramanyam
    Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California San Francisco, San Francisco, California, USA
    Nat Biotechnol 29:443-8. 2011
    ..We propose that individual miRNAs generally act through numerous pathways that synergize to regulate and enforce cell fate decisions...
  26. pmc Small RNAs in early mammalian development: from gametes to gastrulation
    Nayoung Suh
    The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, Center for Reproductive Sciences, and Department of Urology, University of California San Francisco, San Francisco, CA 94143, USA
    Development 138:1653-61. 2011
    ..Here, we review the roles of small non-coding RNAs during the early stages of mammalian development, from gamete maturation through to gastrulation...
  27. ncbi request reprint Two miRNA Clusters Reveal Alternative Paths in Late-Stage Reprogramming
    Ronald J Parchem
    The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, Center for Reproductive Sciences, University of California, San Francisco, San Francisco, California, 94143, USA Department of Urology, University of California, San Francisco, San Francisco, California, 94143, USA
    Cell Stem Cell 14:617-31. 2014
    ..These results demonstrate that cells can follow multiple paths during the late stages of reprogramming, and that the trajectory of any individual cell is strongly influenced by the combination of factors introduced. ..
  28. ncbi request reprint Small RNAs in germline development
    Matthew S Cook
    Department of Urology, University of California, San Francisco, California, USA
    Curr Top Dev Biol 102:159-205. 2013
    ..Here, we review current knowledge of the roles miRNAs, endo-siRNAs, and piRNAs play at all stages of germline development in various organisms, focusing on studies in the mouse...
  29. ncbi request reprint miR-19, miR-345, miR-519c-5p Serum Levels Predict Adverse Pathology in Prostate Cancer Patients Eligible for Active Surveillance
    Siao Yi Wang
    Department of Urology, Helen Diller Family Comprehensive Cancer Center, University of California San Francisco, San Francisco, California, United States of America
    PLoS ONE 9:e98597. 2014
    ..It will be important to validate these findings in a larger multi-institutional study as well as with independent methodologies. ..
  30. doi request reprint MicroRNA Regulation of Embryonic Stem Cell Self-Renewal and Differentiation
    Collin Melton
    The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, Center for Reproductive Sciences, Biomedical Science Graduate Program and Department of Urology, University of California San Francisco, San Francisco, California, 94143, USA
    Adv Exp Med Biol 695:105-17. 2010
    ..We will compare and contrast miRNA functions in ESCs with miRNA function in lineage specific somatic stem cells and in cancer...

Research Grants7

  1. Determinants of Neural & Embryonic Stem Cell Potency
    Robert Blelloch; Fiscal Year: 2007
    ..This line of research should provide conceptual and therapeutic insight into means for altering stem cell fates for the amelioration of genetic and acquired neuropathologic disease. ..
  2. Small RNAs in Stem Cell Differentiation and Neural Development
    Robert Blelloch; Fiscal Year: 2007
    ..Therefore, a systematic analysis of their function in embryonic and somatic stem cells as proposed in this grant should provide important insights into how they influence and can be used to diagnose and treat human disease. ..
  3. Small RNAs in Stem Cell Differentiation and Neural Development
    Robert Blelloch; Fiscal Year: 2009
    ..Therefore, a systematic analysis of their function in embryonic and somatic stem cells as proposed in this grant should provide important insights into how they influence and can be used to diagnose and treat human disease. ..
  4. Small RNAs in Stem Cell Differentiation and Neural Development
    Robert H Blelloch; Fiscal Year: 2010
    ..Therefore, a systematic analysis of their function in embryonic and somatic stem cells as proposed in this grant should provide important insights into how they influence and can be used to diagnose and treat human disease. ..
  5. Small RNAs in Stem Cell Differentiation and Neural Development
    Robert H Blelloch; Fiscal Year: 2010
    ..Therefore, a systematic analysis of their function in embryonic and somatic stem cells as proposed in this grant should provide important insights into how they influence and can be used to diagnose and treat human disease. ..