Pilar Blancafort

Summary

Affiliation: University of North Carolina
Country: USA

Publications

  1. pmc Suppression of breast tumor growth and metastasis by an engineered transcription factor
    Adriana S Beltran
    Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America
    PLoS ONE 6:e24595. 2011
  2. pmc Targeted silencing of the oncogenic transcription factor SOX2 in breast cancer
    Sabine Stolzenburg
    Epigenetic Editing, Department of Pathology and Medical Biology, University Medical Center Groningen, University of Groningen, Hanzeplein 1, 9713 GZ Groningen, The Netherlands
    Nucleic Acids Res 40:6725-40. 2012
  3. ncbi request reprint Rational design, selection and specificity of artificial transcription factors (ATFs): the influence of chromatin in target gene regulation
    Pilar Blancafort
    Department of Pharmacology and the Lineberger Comprehensive Cancer Center, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7365, USA
    Comb Chem High Throughput Screen 11:146-58. 2008
  4. pmc Breaking through an epigenetic wall: re-activation of Oct4 by KRAB-containing designer zinc finger transcription factors
    Karla Juárez-Moreno
    Department of Pharmacology, University of North Carolina School of Medicine, Chapel Hill, NC, USA
    Epigenetics 8:164-76. 2013
  5. pmc Targeting serous epithelial ovarian cancer with designer zinc finger transcription factors
    Haydee Lara
    Department of Pharmacology, Eshelman School of Pharmacy, University of North Carolina, Chapel Hill, NC 27599, USA
    J Biol Chem 287:29873-86. 2012
  6. doi request reprint Reprogramming epigenetic silencing: artificial transcription factors synergize with chromatin remodeling drugs to reactivate the tumor suppressor mammary serine protease inhibitor
    Adriana S Beltran
    Department of Pharmacology and Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7365, USA
    Mol Cancer Ther 7:1080-90. 2008
  7. pmc Reactivation of MASPIN in non-small cell lung carcinoma (NSCLC) cells by artificial transcription factors (ATFs)
    Adriana S Beltran
    Department of Pharmacology, The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
    Epigenetics 6:224-35. 2011
  8. doi request reprint Remodeling genomes with artificial transcription factors (ATFs)
    Adriana S Beltran
    Department of Pharmacology, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
    Methods Mol Biol 649:163-82. 2010
  9. pmc Generation of tumor-initiating cells by exogenous delivery of OCT4 transcription factor
    Adriana S Beltran
    Department of Pharmacology, The University of North Carolina at Chapel Hill, 120 Mason Farm Road, Chapel Hill, NC 27599, USA
    Breast Cancer Res 13:R94. 2011
  10. ncbi request reprint Interrogating genomes with combinatorial artificial transcription factor libraries: asking zinc finger questions
    Adriana Beltran
    Department of Pharmacology, University of North Carolina, Chapel Hill, NC 27599, USA
    Assay Drug Dev Technol 4:317-31. 2006

Collaborators

  • David J Segal
  • Yuhua Wang
  • Leaf Huang
  • Cheng Fan
  • Yanzheng Liu
  • Denise C Connolly
  • Yunxia Hu
  • Yang Yang
  • Adriana S Beltran
  • Karla Juárez-Moreno
  • Sabine Stolzenburg
  • Xinni Yuan
  • Haydee Lara
  • Haili Qian
  • Carlos F Barbas
  • Ashley G Rivenbark
  • Caren V Lund
  • Paul M Lizardi
  • Adriana Beltran
  • Birgit Dreier
  • Laurent Magnenat
  • Mauricio Cuello-Fredes
  • Gareth I Owen
  • Rafaela Erices
  • Alexei F Licea
  • Marianne G Rots
  • Andrea V Leisewitz
  • Brian D Strahl
  • Sumie Kato
  • Mauricio Cuello Fredes
  • John P Hunt
  • Angela Russo
  • Lee M Graves
  • Haili Quian
  • Eric Zimmerman
  • Bryan T Richardson
  • Xueguang Sun
  • Brenda Temple
  • Shaunak Parikh
  • Roberta P Fuller
  • Adrian Huber
  • Beate Koksch
  • Mikhail Popkov

Detail Information

Publications19

  1. pmc Suppression of breast tumor growth and metastasis by an engineered transcription factor
    Adriana S Beltran
    Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America
    PLoS ONE 6:e24595. 2011
    ..Our results describe for the first time an ATF able to reduce tumor growth and metastatic colonization by epigenetic reactivation of a dormant, normal-like, and more differentiated gene program...
  2. pmc Targeted silencing of the oncogenic transcription factor SOX2 in breast cancer
    Sabine Stolzenburg
    Epigenetic Editing, Department of Pathology and Medical Biology, University Medical Center Groningen, University of Groningen, Hanzeplein 1, 9713 GZ Groningen, The Netherlands
    Nucleic Acids Res 40:6725-40. 2012
    ..Collectively, these findings demonstrate the effectiveness and therapeutic potential of engineered ATFs to mediate potent and long-lasting down-regulation of oncogenic TF expression in cancer cells...
  3. ncbi request reprint Rational design, selection and specificity of artificial transcription factors (ATFs): the influence of chromatin in target gene regulation
    Pilar Blancafort
    Department of Pharmacology and the Lineberger Comprehensive Cancer Center, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7365, USA
    Comb Chem High Throughput Screen 11:146-58. 2008
    ..The in vivo specificity of the engineered ATFs will be discussed, with particular emphasis on epigenetic modifications influencing ATF-DNA interactions...
  4. pmc Breaking through an epigenetic wall: re-activation of Oct4 by KRAB-containing designer zinc finger transcription factors
    Karla Juárez-Moreno
    Department of Pharmacology, University of North Carolina School of Medicine, Chapel Hill, NC, USA
    Epigenetics 8:164-76. 2013
    ..In addition, we document a novel ZFP that could be used for the epigenetic reprograming of cancer cells...
  5. pmc Targeting serous epithelial ovarian cancer with designer zinc finger transcription factors
    Haydee Lara
    Department of Pharmacology, Eshelman School of Pharmacy, University of North Carolina, Chapel Hill, NC 27599, USA
    J Biol Chem 287:29873-86. 2012
    ..Our work outlined the first targeted, non-viral delivery of ATFs into tumors with potential clinical applications for metastatic ovarian cancers...
  6. doi request reprint Reprogramming epigenetic silencing: artificial transcription factors synergize with chromatin remodeling drugs to reactivate the tumor suppressor mammary serine protease inhibitor
    Adriana S Beltran
    Department of Pharmacology and Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7365, USA
    Mol Cancer Ther 7:1080-90. 2008
    ..Our results document the power of a novel therapeutic approach that combines both epigenetic and genetic (sequence-specific ATFs) strategies to reactivate specifically silenced regions of the genome and reprogram cellular phenotypes...
  7. pmc Reactivation of MASPIN in non-small cell lung carcinoma (NSCLC) cells by artificial transcription factors (ATFs)
    Adriana S Beltran
    Department of Pharmacology, The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
    Epigenetics 6:224-35. 2011
    ..These data suggest that ATFs are able to reprogram aggressive lung tumor cells towards a more epithelial, differentiated phenotype, and thus, represent novel therapeutic agents for metastatic lung cancers...
  8. doi request reprint Remodeling genomes with artificial transcription factors (ATFs)
    Adriana S Beltran
    Department of Pharmacology, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
    Methods Mol Biol 649:163-82. 2010
    ..Herein, we describe a new method, which combines artificial transcription factors (ATFs) and chromatin-remodeling agents to specifically reactivate silenced regions of the genome and reprogram cellular phenotypes...
  9. pmc Generation of tumor-initiating cells by exogenous delivery of OCT4 transcription factor
    Adriana S Beltran
    Department of Pharmacology, The University of North Carolina at Chapel Hill, 120 Mason Farm Road, Chapel Hill, NC 27599, USA
    Breast Cancer Res 13:R94. 2011
    ..Herein, we describe the generation of TIC-like cell lines by ectopic expression of the OCT4 transcription factor (TF) in primary breast cell preparations...
  10. ncbi request reprint Interrogating genomes with combinatorial artificial transcription factor libraries: asking zinc finger questions
    Adriana Beltran
    Department of Pharmacology, University of North Carolina, Chapel Hill, NC 27599, USA
    Assay Drug Dev Technol 4:317-31. 2006
    ..In this review we will summarize several applications of ATF library screenings in gene discovery, biotechnology, and disease therapeutics...
  11. pmc Systemic delivery of modified mRNA encoding herpes simplex virus 1 thymidine kinase for targeted cancer gene therapy
    Yuhua Wang
    Division of Molecular Pharmaceutics and Center for Nanotechnology in Drug Delivery, Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Mol Ther 21:358-67. 2013
    ....
  12. ncbi request reprint Scanning the human genome with combinatorial transcription factor libraries
    Pilar Blancafort
    Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Nat Biotechnol 21:269-74. 2003
    ..Transcription factor libraries represent a useful approach for studying and modulating gene function in cells and potentially in whole organisms...
  13. ncbi request reprint Designing transcription factor architectures for drug discovery
    Pilar Blancafort
    Department of Molecular Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Mol Pharmacol 66:1361-71. 2004
    ....
  14. ncbi request reprint In vivo selection of combinatorial libraries and designed affinity maturation of polydactyl zinc finger transcription factors for ICAM-1 provides new insights into gene regulation
    Laurent Magnenat
    The Skaggs Institute for Chemical Biology and the Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    J Mol Biol 341:635-49. 2004
    ..Transcription factor libraries provide genome-wide approaches that can be applied towards the development of TFZFs specific for virtually any gene or desired phenotype and may lead to the discovery of new genetic functions and pathways...
  15. ncbi request reprint Development of zinc finger domains for recognition of the 5'-CNN-3' family DNA sequences and their use in the construction of artificial transcription factors
    Birgit Dreier
    The Skaggs Institute for Chemical Biology, La Jolla, California 92037, USA
    J Biol Chem 280:35588-97. 2005
    ..With the addition of this collection of predefined zinc finger domains, most 5'-CNN-3'-, 5'-GNN-3'-, and 5'-ANN-3'-containing sequences can now be rapidly targeted for directed gene regulation and nuclease cleavage...
  16. pmc Genetic reprogramming of tumor cells by zinc finger transcription factors
    Pilar Blancafort
    Department of Molecular Biology and The Skaggs Institute for Chemical Biology, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 102:11716-21. 2005
    ..By co-regulating multiple targets, ATFs can be used as master genetic switches to reprogram and modulate complex neoplastic phenotypes...
  17. ncbi request reprint Evaluation of a modular strategy for the construction of novel polydactyl zinc finger DNA-binding proteins
    David J Segal
    The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Biochemistry 42:2137-48. 2003
    ..These results have important implications for the design of proteins that can recognize extended DNA sequences, as well as provide insights into the general rules of recognition for naturally occurring zinc finger proteins...
  18. ncbi request reprint Promoter-targeted phage display selections with preassembled synthetic zinc finger libraries for endogenous gene regulation
    Caren V Lund
    The Skaggs Institute for Chemical Biology and Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    J Mol Biol 340:599-613. 2004
    ....
  19. doi request reprint Modulation of drug resistance by artificial transcription factors
    Pilar Blancafort
    Department of Molecular Biology and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Mol Cancer Ther 7:688-97. 2008
    ..Thus, ATF can be used to map genes and pathways involved in drug resistance phenotypes and have potential as novel therapeutic agents to inhibit drug resistance...

Research Grants5

  1. Re-activation of maspin tumor suppressor gene by designed transcription factors
    Pilar Blancafort; Fiscal Year: 2007
    ..This work should lead to the characterization of novel anti-cancer regulators, able to reprogram the aberrant epigenetic silencing of tumor suppressors ..
  2. Re-activation of maspin tumor suppressor gene by designed transcription factors
    Pilar Blancafort; Fiscal Year: 2009
    ..This work should lead to the characterization of novel anti-cancer regulators, able to reprogram the aberrant epigenetic silencing of tumor suppressors ..
  3. Re-activation of maspin tumor suppressor gene by designed transcription factors
    Pilar Blancafort; Fiscal Year: 2010
    ..This work should lead to the characterization of novel anti-cancer regulators, able to reprogram the aberrant epigenetic silencing of tumor suppressors ..
  4. Re-activation of maspin tumor suppressor gene by designed transcription factors
    Pilar Blancafort; Fiscal Year: 2009
    ..This work should lead to the characterization of novel anti-cancer regulators, able to reprogram the aberrant epigenetic silencing of tumor suppressors ..