I A Blair

Summary

Affiliation: University of Pennsylvania
Country: USA

Publications

  1. ncbi request reprint Analysis of anticancer drugs and their metabolites by mass spectrometry
    Ian A Blair
    Center for Cancer Pharmacology, University of Pennsylvania School of Medicine, 1254 BRB II III, 421 Curie Boulevard, Philadelphia, PA 19104 6160, USA
    Curr Drug Metab 3:463-80. 2002
  2. ncbi request reprint Endogenous glutathione adducts
    Ian A Blair
    Center for Cancer Pharmacology and Institute for Translational Medicine and Therapeutics, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6160, USA
    Curr Drug Metab 7:853-72. 2006
  3. pmc Stable isotope dilution multidimensional liquid chromatography-tandem mass spectrometry for pancreatic cancer serum biomarker discovery
    Kenneth H Yu
    Centers for Cancer Pharmacology and Excellence in Environmental Toxicology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    J Proteome Res 8:1565-76. 2009
  4. ncbi request reprint Lipid hydroperoxide-mediated DNA damage
    I A Blair
    Center for Cancer Pharmacology, University of Pennsylvania, 1254 BRB II III, 421 Curie Boulevard, Philadelphia, PA 19104 6160, USA
    Exp Gerontol 36:1473-81. 2001
  5. pmc DNA adducts with lipid peroxidation products
    Ian A Blair
    Center for Cancer Pharmacology and Excellence in Environmental Toxicology and the Institute for Translational Medicine and Therapeutics, University of Pennsylvania, Philadelphia, Pennsylvania 19104 6160, USA
    J Biol Chem 283:15545-9. 2008
  6. ncbi request reprint Vitamin C-induced decomposition of lipid hydroperoxides to endogenous genotoxins
    S H Lee
    Center for Cancer Pharmacology, University of Pennsylvania, 1254 BRB II/III, 421 Curie Boulevard, Philadelphia, PA 19104-6160, USA
    Science 292:2083-6. 2001
  7. pmc Cyclooxygenase- and lipoxygenase-mediated DNA damage
    N Speed
    Centers for Cancer Pharmacology and Excellence in Environmental Toxicology, University of Pennsylvania Perelman School of Medicine, 854 BRB II III, 421 Curie Boulevard, Philadelphia, PA 19104 6160, USA
    Cancer Metastasis Rev 30:437-47. 2011
  8. pmc Analysis of endogenous glutathione-adducts and their metabolites
    Ian A Blair
    Centers of Excellence in Environmental Toxicology and Cancer Pharmacology, Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 4863, USA
    Biomed Chromatogr 24:29-38. 2010
  9. pmc Liquid chromatography-mass spectrometry (LC-MS) of steroid hormone metabolites and its applications
    Trevor M Penning
    Centers of Excellence in Environmental Toxicology and Cancer Pharmacology, Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6084, USA
    J Steroid Biochem Mol Biol 121:546-55. 2010
  10. pmc The post-synaptic density of human postmortem brain tissues: an experimental study paradigm for neuropsychiatric illnesses
    Chang Gyu Hahn
    Department of Psychiatry, University of Pennsylvania, Philadelphia, PA, USA
    PLoS ONE 4:e5251. 2009

Collaborators

  • Seon Hwa Lee
  • TREVOR PENNING
  • Wenying Jian
  • Anastasia K Yocum
  • G A Fitzgerald
  • D W Roberts
  • Koichi Inoue
  • Douglas L Fraker
  • Chang Gyu Hahn
  • Xiaoliang Zhuo
  • STEVEN ARNOLD
  • Michael Berna
  • Hoau Yan Wang
  • N Osheroff
  • F Cipollone
  • Chandrakala Menon
  • Tomoyuki Oe
  • Kenneth H Yu
  • Ronald G Harvey
  • Michael Pollack
  • Peijuan Zhu
  • Dipti Mangal
  • Yuehua Huang
  • Eugene Ciccimaro
  • Qian Ruan
  • Michelle V Williams
  • Vineet Sangar
  • Anil K Rustgi
  • Margaret B Lucitt
  • Thomas S Price
  • Hao Jiang
  • N Speed
  • Nisha T Palackal
  • Michael C Byrns
  • Ling Duan
  • Yi Jin
  • Clementina Mesaros
  • Kannan Rangiah
  • Sumit J Shah
  • David Austin
  • Carol A Shultz
  • Tilo Grosser
  • Weichen Wu
  • Angel Pizarro
  • Alexander S Whitehead
  • Stacy L Gelhaus
  • Caterina Pettinella
  • Andrés Trostchansky
  • Naiyu Zheng
  • Hye Young H Kim
  • Reshma F Kurangi
  • Carolyn A Felix
  • Yuan Yan
  • Lawrence M Szewczuk
  • Anita R Mistry
  • Byron H Arison
  • Todd W Bauer
  • Ye Tian
  • PETER J O'DWYER
  • Christopher J Bailey
  • Christine M Busch
  • Jong Heum Park
  • Daljit Vudathala
  • Marie Pier Tetreault
  • SAMUEL I PARRY
  • Colin G Barry
  • Montri Tippornwong
  • Steven K Hanks
  • Helenius J Kloosterboer
  • Christoph Seiler
  • Stefanie Khartulyari
  • Megan E Morales
  • Michael A Pack
  • Anna Stanislawska-Sachadyn
  • Joan M Von Feldt
  • David J Austin
  • Karen S Brown
  • Zhi Yong Lu
  • Summer Lind
  • Valentina Gelfanova
  • Shaokun Pang
  • Martin R Farlow
  • Eric R Siemers
  • Santosh G Tilve
  • Homero Rubbo
  • David M Holtzman
  • Carlos O Garner
  • In Young Yang
  • Suzanne Wehrli
  • Masaaki Moriya

Detail Information

Publications65

  1. ncbi request reprint Analysis of anticancer drugs and their metabolites by mass spectrometry
    Ian A Blair
    Center for Cancer Pharmacology, University of Pennsylvania School of Medicine, 1254 BRB II III, 421 Curie Boulevard, Philadelphia, PA 19104 6160, USA
    Curr Drug Metab 3:463-80. 2002
    ..The need to analyze anticancer drugs and their metabolites with such prompt turn around times has stimulated even more rapid approaches to analysis using robotic-based purification methodology and short LC chromatographic run times...
  2. ncbi request reprint Endogenous glutathione adducts
    Ian A Blair
    Center for Cancer Pharmacology and Institute for Translational Medicine and Therapeutics, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6160, USA
    Curr Drug Metab 7:853-72. 2006
    ..In future studies, it will also be important to establish the biological activity of TOG and its analogs in view of the potent activity of many other endogenous GSH-adducts such as the leukotrienes...
  3. pmc Stable isotope dilution multidimensional liquid chromatography-tandem mass spectrometry for pancreatic cancer serum biomarker discovery
    Kenneth H Yu
    Centers for Cancer Pharmacology and Excellence in Environmental Toxicology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    J Proteome Res 8:1565-76. 2009
    ..This technique allows for the detection of low-abundance proteins, and focuses only on biologically relevant proteins derived from pancreatic cancer cells...
  4. ncbi request reprint Lipid hydroperoxide-mediated DNA damage
    I A Blair
    Center for Cancer Pharmacology, University of Pennsylvania, 1254 BRB II III, 421 Curie Boulevard, Philadelphia, PA 19104 6160, USA
    Exp Gerontol 36:1473-81. 2001
    ..Such studies will help elucidate the role of lipid hydroperoxide-derived endogenous DNA as mediators of cancer,..
  5. pmc DNA adducts with lipid peroxidation products
    Ian A Blair
    Center for Cancer Pharmacology and Excellence in Environmental Toxicology and the Institute for Translational Medicine and Therapeutics, University of Pennsylvania, Philadelphia, Pennsylvania 19104 6160, USA
    J Biol Chem 283:15545-9. 2008
  6. ncbi request reprint Vitamin C-induced decomposition of lipid hydroperoxides to endogenous genotoxins
    S H Lee
    Center for Cancer Pharmacology, University of Pennsylvania, 1254 BRB II/III, 421 Curie Boulevard, Philadelphia, PA 19104-6160, USA
    Science 292:2083-6. 2001
    ..Vitamin C-mediated formation of genotoxins from lipid hydroperoxides in the absence of transition metal ions could help explain its lack of efficacy as a cancer chemoprevention agent...
  7. pmc Cyclooxygenase- and lipoxygenase-mediated DNA damage
    N Speed
    Centers for Cancer Pharmacology and Excellence in Environmental Toxicology, University of Pennsylvania Perelman School of Medicine, 854 BRB II III, 421 Curie Boulevard, Philadelphia, PA 19104 6160, USA
    Cancer Metastasis Rev 30:437-47. 2011
    ..As approximately 80% of cancers arise from somatic mutations, this provides an additional link between the upregulation of COX-2 and tumorigenesis...
  8. pmc Analysis of endogenous glutathione-adducts and their metabolites
    Ian A Blair
    Centers of Excellence in Environmental Toxicology and Cancer Pharmacology, Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 4863, USA
    Biomed Chromatogr 24:29-38. 2010
    ..It will also discuss potential future directions for the use of this methodology for rigorous determination of their utility as disease and exposure biomarkers...
  9. pmc Liquid chromatography-mass spectrometry (LC-MS) of steroid hormone metabolites and its applications
    Trevor M Penning
    Centers of Excellence in Environmental Toxicology and Cancer Pharmacology, Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6084, USA
    J Steroid Biochem Mol Biol 121:546-55. 2010
    ..Our LC-MS methods have sufficient sensitivity to detect steroid hormone levels in prostate and breast tumors and should aid their molecular diagnosis and treatment...
  10. pmc The post-synaptic density of human postmortem brain tissues: an experimental study paradigm for neuropsychiatric illnesses
    Chang Gyu Hahn
    Department of Psychiatry, University of Pennsylvania, Philadelphia, PA, USA
    PLoS ONE 4:e5251. 2009
    ..This approach may foster novel postmortem brain research paradigms in which the stoichiometry and protein composition of specific microdomains are examined...
  11. pmc Human cytosolic hydroxysteroid dehydrogenases of the aldo-ketoreductase superfamily catalyze reduction of conjugated steroids: implications for phase I and phase II steroid hormone metabolism
    Yi Jin
    Centers of Excellence in Environmental Toxicology and Cancer Pharmacology, Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 6084, USA
    J Biol Chem 284:10013-22. 2009
    ....
  12. pmc 5-Lipoxygenase-mediated endogenous DNA damage
    Wenying Jian
    Center for Cancer Pharmacology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 6160, USA
    J Biol Chem 284:16799-807. 2009
    ..These data showed that 5-LO was the enzyme responsible for the generation of the HepsilondGuo DNA-adduct in CESS cells...
  13. ncbi request reprint Induction of endothelial cell apoptosis by lipid hydroperoxide-derived bifunctional electrophiles
    Wenying Jian
    Center for Cancer Pharmacology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6160, USA
    Free Radic Biol Med 39:1162-76. 2005
    ..These data suggest that lipid hydroperoxide-derived bifunctional electrophiles may play an important role in cardiovascular pathology through their ability to induce endothelial cell apoptosis...
  14. ncbi request reprint Translesion DNA Synthesis across the heptanone--etheno-2'-deoxycytidine adduct in cells
    Michael Pollack
    Center for Cancer Pharmacology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Chem Res Toxicol 19:1074-9. 2006
    ..coli, while dA and dT were the preferential choices in human cells. These results indicate that this endogenous DNA adduct is very genotoxic to both organisms...
  15. ncbi request reprint A novel 4-oxo-2(E)-nonenal-derived endogenous thiadiazabicyclo glutathione adduct formed during cellular oxidative stress
    Wenying Jian
    Centers for Cancer Pharmacology and Excellence in Environmental Toxicology, University of Pennsylvania School of Medicine, 854 BRB II III, 421 Curie Boulevard, Philadelphia Pennsylvania 19104 6160, USA
    Chem Res Toxicol 20:1008-18. 2007
    ..ONO is isomeric with HNE; therefore, protein and peptide adducts ascribed to arise solely from reactions with endogenous HNE will need to be re-appraised...
  16. ncbi request reprint 4,5-Epoxy-2(E)-decenal-induced formation of 1,N(6)-etheno-2'-deoxyadenosine and 1,N(2)-etheno-2'-deoxyguanosine adducts
    Seon Hwa Lee
    Center for Cancer Pharmacology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 6160, USA
    Chem Res Toxicol 15:300-4. 2002
    ..These findings provide an important link between a primary product of lipid peroxidation and a mutagenic DNA lesion that has been detected in human tissues...
  17. ncbi request reprint Characterization of 2'-deoxycytidine adducts derived from 4-oxo-2-nonenal, a novel lipid peroxidation product
    Michael Pollack
    Center for Cancer Pharmacology, University of Pennsylvania School of Medicine, 1254 BRB II III, 421 Curie Boulevard, Philadelphia, Pennsylvania 19104 6160, UK
    Chem Res Toxicol 16:893-900. 2003
    ..The data are consistent with adducts A(1) and A(2) being substituted ethano adducts that dehydrate to adduct B, a substituted 3,N(4)-etheno-2'-deoxycytidine adduct...
  18. ncbi request reprint Endogenous lipid hydroperoxide-mediated DNA-adduct formation in min mice
    Michelle V Williams
    Center for Cancer Pharmacology, University of Pennsylvania, 854 BRB II III, 421 Curie Boulevard, Philadelphia, PA 19104 6160, USA
    J Biol Chem 281:10127-33. 2006
    ....
  19. ncbi request reprint Oxidative DNA damage and cardiovascular disease
    S H Lee
    Center for Cancer Pharmacology, 1254 BRB II/III, 421 Curie Bvd, University of Pennsylvania, Philadelphia, PA 19104-6160, USA
    Trends Cardiovasc Med 11:148-55. 2001
    ..Very little is known about the consequences of lipid hydroperoxide-mediated DNA damage in cardiovascular diseases. This should prove to be an important area for future research...
  20. ncbi request reprint Quantification of benzo[a]pyrene diol epoxide DNA-adducts by stable isotope dilution liquid chromatography/tandem mass spectrometry
    Qian Ruan
    Center for Cancer Pharmacology, University of Pennsylvania School of Medicine, 854 BRB II III, 421 Curie Boulevard, Philadelphia, PA 19104 6160, USA
    Rapid Commun Mass Spectrom 20:1369-80. 2006
    ..Also, the adduct profile suggests that this occurs by binding of (+)-anti-B[a]PDE to DNA in a manner that facilitates covalent binding to dGuo rather than dAdo residues...
  21. pmc Absolute quantification of phosphorylation on the kinase activation loop of cellular focal adhesion kinase by stable isotope dilution liquid chromatography/mass spectrometry
    Eugene Ciccimaro
    Center for Cancer Pharmacology, Department of Pharmacology, University of Pennsylvania, 854 BRB II III, 421 Curie Boulevard, Philadelphia, Pennsylvania 19104 6160, USA
    Anal Chem 81:3304-13. 2009
    ..This methodology will have particular utility for biomarker studies of kinase-inhibiting anticancer drugs and for quantitative proteomic investigations that examine kinase- and phosphatase-mediated cellular signal transduction pathways...
  22. pmc Analysis of 7,8-dihydro-8-oxo-2'-deoxyguanosine in cellular DNA during oxidative stress
    Dipti Mangal
    Centers for Cancer Pharmacology and Excellence in Environmental Toxicology, University of Pennsylvania, 854 BRB II III, 421 Curie Boulevard, Philadelphia, Pennsylvania 19104 6160, USA
    Chem Res Toxicol 22:788-97. 2009
    ..In keeping with this concept, inhibition of catechol-O-methyl transferase (COMT)-mediated detoxification of B[a]P-7,8-catechol with Ro 410961 caused increased 8-oxo-dGuo formation in the H358 cell DNA...
  23. ncbi request reprint Dioxododecenoic acid: a lipid hydroperoxide-derived bifunctional electrophile responsible for etheno DNA adduct formation
    Seon Hwa Lee
    Center for Cancer Pharmacology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 6160, USA
    Chem Res Toxicol 18:566-78. 2005
    ..Synthetic DODE when reacted with dGuo gave carboxynonanone-etheno-dGuo that was identical with that derived from vitamin C-mediated 13(S)-HPODE decomposition in the presence of dGuo...
  24. pmc Fjord-region benzo[g]chrysene-11,12-dihydrodiol and benzo[c]phenanthrene-3,4-dihydrodiol as substrates for rat liver dihydrodiol dehydrogenase (AKR1C9): structural basis for stereochemical preference
    Carol A Shultz
    Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    Chem Res Toxicol 21:668-77. 2008
    ..The similarity between rates of trans-dihydrodiol oxidation by the rat and human liver specific AKRs (AKR1C9 and AKR1C4) implicate these enzymes in hepatocarcinogenesis in rats observed with the fjord-region PAH...
  25. ncbi request reprint Characterization of an etoposide-glutathione conjugate derived from metabolic activation by human cytochrome p450
    Naiyu Zheng
    Center for Cancer Pharmacology, University of Pennsylvania School of Medicine, 854 BRB II III, 421 Curie Boulevard, Philadelphia, PA 19104 6160, USA
    Curr Drug Metab 7:897-911. 2006
    ..The oxidation of etoposide-OH to a quinone intermediate is likely the precursor in the formation of etoposide-OH-6'-SG...
  26. ncbi request reprint LC/ESI/MS analysis of saturated and unsaturated fatty acids in rat intestinal epithelial cells
    Seon Hwa Lee
    Center for Cancer Pharmacology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6160, USA
    Curr Drug Metab 7:929-37. 2006
    ..This methodology has allowed the analysis of fatty acid composition from parental rat intestinal epithelial cell and rat intestinal epithelial cells transfected with cyclooxygenase-2, a model system of oxidative stress...
  27. pmc A 4-oxo-2(E)-nonenal-derived glutathione adduct from 15-lipoxygenase-1-mediated oxidation of cytosolic and esterified arachidonic acid
    Peijuan Zhu
    Centers for Cancer Pharmacology and Excellence in Environmental Toxicology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6160, USA
    Free Radic Biol Med 47:953-61. 2009
    ..Thus, it is likely that esterified 15(S)-HpETE underwent homolytic decomposition to 4-oxo-2(E)-nonenal more efficiently than the free 15(S)-HpETE that was formed in the cytosol...
  28. doi request reprint Determination of cellular redox status by stable isotope dilution liquid chromatography/mass spectrometry analysis of glutathione and glutathione disulfide
    Peijuan Zhu
    Centers for Cancer Pharmacology and Excellence in Environmental Toxicology Department of Pharmacology, University of Pennsylvania School of Medicine, 854 BRB II III, 421 Curie Boulevard, Philadelphia, PA 19104 6160, USA
    Rapid Commun Mass Spectrom 22:432-40. 2008
    ..This suggests that increased expression of 15(S)-HPETE modulates the activity of cellular GSH reductases or the transporters involved in removal of GSSG...
  29. ncbi request reprint A validated liquid chromatography/tandem mass spectrometry assay for cis-amminedichloro(2-methylpyridine)platinum(II) in human plasma ultrafiltrate
    Tomoyuki Oe
    Center for Cancer Pharmacology, Department of Pharmacology, University of Pennsylvania, Philadelphia 19104 6160, USA
    Anal Chem 74:591-9. 2002
    ..The method provided adequate sensitivity for the analysis of plasma ultrafiltrate samples from a phase II study in which ZD0473 was administered to patients as an intravenous infusion at a dose of 150 mg/m2...
  30. ncbi request reprint A novel lipid hydroperoxide-derived modification to arginine
    Tomoyuki Oe
    Center for Cancer Pharmacology, University of Pennsylvania School of Medicine, 1254 BRB II III, 421 Curie Boulevard, Philadelphia, Pennsylvania 19104 6160, USA
    Chem Res Toxicol 16:1598-605. 2003
    ..This suggests that arginine-containing proteins can react with lipid hydroperoxide-derived 4-oxo-2-nonenal to form a novel imidazole modification...
  31. ncbi request reprint Kinetics and regulation of cytochrome P450-mediated etoposide metabolism
    Xiaoliang Zhuo
    Center for Cancer Pharmacology, Department of Pharmacology, University of Pennsylvania School of Medicine, 421 Curie Boulevard, Philadelphia, PA 19104 6160, USA
    Drug Metab Dispos 32:993-1000. 2004
    ..Alternatively, coadministration of other drugs with etoposide may account for the increase in etoposide catechol formation during therapy with etoposide...
  32. ncbi request reprint Liquid chromatography/mass spectrometry analysis of bifunctional electrophiles and DNA adducts from vitamin C mediated decomposition of 15-hydroperoxyeicosatetraenoic acid
    Michelle V Williams
    Center for Cancer Pharmacology, University of Pennsylvania School of Medicine, 854 BRB II III, 421 Curie Boulevard, Philadelphia, PA 19104 6160, USA
    Rapid Commun Mass Spectrom 19:849-58. 2005
    ..In contrast to reactions with dGuo or DNA conducted using 13(S)-HPODE, no carboxy-containing adducts were observed with 15(S)-HPETE...
  33. ncbi request reprint Novel lipid hydroperoxide-derived hemoglobin histidine adducts as biomarkers of oxidative stress
    Anastasia K Yocum
    Center for Cancer Pharmacology, University of Pennsylvania School of Medicine, 854 BRB II III, 421 Curie Boulevard, Philadelphia, PA 19104 6160, USA
    J Mass Spectrom 40:754-64. 2005
    ..GK(65) from beta-hemoglobin. These hemoglobin modifications can potentially serve as biomarkers of lipid hydroperoxide-mediated macromolecule damage and may reflect an indirect measurement of the potential for DNA damage in vivo...
  34. ncbi request reprint 4-Hydroperoxy-2-nonenal-induced formation of 1,N2-etheno-2'-deoxyguanosine adducts
    Seon Hwa Lee
    Center for Cancer Pharmacology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 6160, USA
    Chem Res Toxicol 18:780-6. 2005
    ..Therefore, HPNE can be considered as another lipid hydroperoxide-derived bifunctional electrophile with the potential for biological activities that are similar to HNE and ONE...
  35. ncbi request reprint Cyclooxygenase-2-mediated DNA damage
    Seon Hwa Lee
    Center for Cancer Pharmacology, University of Pennsylvania, Philadelphia, Pennsylvania 19104 6160, USA
    J Biol Chem 280:28337-46. 2005
    ..Therefore, inhibition of COX-2-mediated lipid hydroperoxide formation offers a potential therapeutic alternative to COX-2 inhibitors in chemoprevention strategies...
  36. ncbi request reprint Effect of immunoaffinity depletion of human serum during proteomic investigations
    Anastasia K Yocum
    Center for Cancer Pharmacology, University of Pennsylvania School of Medicine, 421 Curie Boulevard, Philadelphia, PA 19104 6160, USA
    J Proteome Res 4:1722-31. 2005
    ..Although serum depletion of highly abundant proteins significantly increased the number of proteins identified, both the degree of sample complexity and this depletion method resulted in a nonselective loss of other proteins...
  37. ncbi request reprint EBP, a program for protein identification using multiple tandem mass spectrometry datasets
    Thomas S Price
    Institute for Translational Medicine and Therapeutics, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Mol Cell Proteomics 6:527-36. 2007
    ..We have implemented this statistical method as an analytic module that can be integrated with an existing suite of open-source proteomics software...
  38. pmc Identification and quantification of preterm birth biomarkers in human cervicovaginal fluid by liquid chromatography/tandem mass spectrometry
    Sumit J Shah
    Center for Excellence in Environmental Toxicology, Department of Obstetrics and Gynecology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 6160, USA
    J Proteome Res 8:2407-17. 2009
    ..Three proteins were significantly elevated in PTB cases (desmoplakin isoform 1, stratifin, and thrombospondin 1 precursor), providing a foundation for further validation in larger patient cohorts...
  39. ncbi request reprint Viniferin formation by COX-1: evidence for radical intermediates during co-oxidation of resveratrol
    Lawrence M Szewczuk
    Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    J Nat Prod 68:36-42. 2005
    ..Methoxy-resveratrol analogues, in which either the m-hydroquinone or the phenol moiety were protected as methyl ethers, were used to confirm the proposed mechanism of viniferin production by COX-1...
  40. pmc Targeted quantitative analysis of eicosanoid lipids in biological samples using liquid chromatography-tandem mass spectrometry
    Clementina Mesaros
    Center for Cancer Pharmacology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6160, USA
    J Chromatogr B Analyt Technol Biomed Life Sci 877:2736-45. 2009
    ..Several examples of targeted eicosanoid lipid analysis using conventional LC-ESI/MS have been discussed and some new data on the analysis of eicosanoids using chiral LC-ECAPCI/MS has been presented...
  41. ncbi request reprint Characterization of proteins in human pancreatic cancer serum using differential gel electrophoresis and tandem mass spectrometry
    Kenneth H Yu
    Division of Hematology Oncology, Center for Cancer Pharmacology, and Genomics Institute, University of Pennsylvania, 421 Curie Boulevard, Philadelphia, PA 19104 6160, USA
    J Proteome Res 4:1742-51. 2005
    ..These results suggest that affinity column enrichment and 2-D DIGE can be used to identify numerous proteins differentially expressed in serum from individuals with pancreatic cancer...
  42. ncbi request reprint Quantitative analysis of amyloid beta peptides in cerebrospinal fluid of Alzheimer's disease patients by immunoaffinity purification and stable isotope dilution liquid chromatography/negative electrospray ionization tandem mass spectrometry
    Tomoyuki Oe
    Centers for Cancer Pharmacology and Excellence in Environmental Toxicology, Departments of Pharmacology and Chemistry, University of Pennsylvania, Philadelphia, PA 19104 6160, USA
    Rapid Commun Mass Spectrom 20:3723-35. 2006
    ..Moreover, the introduction of stable isotope labeled internal standards offers the potential to achieve a more rigorous account of the influence of methodological effects related to sample collection and processing...
  43. ncbi request reprint Analysis of protein expression during oxidative stress in breast epithelial cells using a stable isotope labeled proteome internal standard
    Yuan Yan
    Center for Cancer Pharmacology, University of Pennsylvania, Philadelphia, Pennsylvania 19104 6160, USA
    J Proteome Res 4:2007-14. 2005
    ..These results suggest that the malignant T4-2 cells have acquired more resistance to H(2)O(2)-induced apoptosis than the nonmalignant S-1 cells...
  44. ncbi request reprint A novel lipid hydroperoxide-derived cyclic covalent modification to histone H4
    Tomoyuki Oe
    Center for Cancer Pharmacology, 1254 BRB II III, University of Pennsylvania, 421 Curie Boulevard, Philadelphia, PA 19104 6160, USA
    J Biol Chem 278:42098-105. 2003
    ..Furthermore, the ability to synthesize cyclic peptides using 4-oxo-2-nonenal will facilitate the preparation of novel structural analogs with potential biological activity...
  45. pmc Differential secreted proteome approach in murine model for candidate biomarker discovery in colon cancer
    Kannan Rangiah
    Centers for Cancer Pharmacology and Excellence in Environmental Toxicology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 6160, USA
    J Proteome Res 8:5153-64. 2009
    ..Differential secretion into the serum was observed for several proteins when Apc(min) mice were compared with control mice. These findings were then confirmed by Western blot analysis...
  46. ncbi request reprint A human melanoma xenograft in a nude rat responds to isolated limb perfusion with TNF plus melphalan
    Todd W Bauer
    Division of Surgical Oncology, Department of Surgery, Hospital of the University of Pennsylvania, 3400 Spruce Street, Philadelphia, PA 19104, USA
    Surgery 133:420-8. 2003
    ..To study molecular mechanisms underlying this response, a nude rat ILP model with a human melanoma xenograft was developed...
  47. pmc Aldo-keto reductase 1C3 expression in MCF-7 cells reveals roles in steroid hormone and prostaglandin metabolism that may explain its over-expression in breast cancer
    Michael C Byrns
    Centers of Excellence in Environmental Toxicology and Cancer Pharmacology, Department of Pharmacology, University of Pennsylvania School of Medicine, 130C John Morgan Bldg, 3620 Hamilton Walk, Philadelphia, PA 19104 6084, United States
    J Steroid Biochem Mol Biol 118:177-87. 2010
    ....
  48. ncbi request reprint Activation of polycyclic aromatic hydrocarbon trans-dihydrodiol proximate carcinogens by human aldo-keto reductase (AKR1C) enzymes and their functional overexpression in human lung carcinoma (A549) cells
    Nisha T Palackal
    Departments of Biochemistry and Biophysics and Pharmacology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    J Biol Chem 277:24799-808. 2002
    ..The ability to measure DMBA-3,4-dione formation in A549 cells implicates the AKR pathway in the metabolic activation of PAH in human lung...
  49. pmc Analysis of estrogens in serum and plasma from postmenopausal women: past present, and future
    Ian A Blair
    Centers of Excellence in Environmental Toxicology and Cancer Pharmacology, Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6084, USA
    Steroids 75:297-306. 2010
    ..This will help conserve important plasma and serum samples as it will be possible to conduct high sensitivity analyses using low sample volumes...
  50. ncbi request reprint Analysis of phosphorylation sites on focal adhesion kinase using nanospray liquid chromatography/multiple reaction monitoring mass spectrometry
    Eugene Ciccimaro
    Center for Cancer Pharmacology, Department of Pharmacology, University of Pennsylvania, 854 BRB II III, 421 Curie Boulevard, Philadelphia, PA 19104 6160, USA
    Rapid Commun Mass Spectrom 20:3681-92. 2006
    ..These findings will impact significantly on future studies of FAK activity since pY397 is often used as a measure of FAK activity and Src association...
  51. ncbi request reprint Analysis of FeII-mediated decomposition of a linoleic acid-derived lipid hydroperoxide by liquid chromatography/mass spectrometry
    Seon Hwa Lee
    Center for Cancer Pharmacology, University of Pennsylvania School of Medicine, Philadelphia, 19104 6160, USA
    J Mass Spectrom 40:661-8. 2005
    ..The HNE is thought to arise from a one-electron reduction of an alkoxy radical derived from HPNE. In contrast, ONE can arise through an alpha-cleavage of the HPNE-derived alkoxy radical or by direct dehydration of HPNE...
  52. ncbi request reprint Quantification of key red blood cell folates from subjects with defined MTHFR 677C>T genotypes using stable isotope dilution liquid chromatography/mass spectrometry
    Yuehua Huang
    Center for Cancer Pharmacology, University of Pennsylvania School of Medicine, 854 BRB II III, 421 Curie Boulevard, Philadelphia, PA 19104 6160, USA
    Rapid Commun Mass Spectrom 22:2403-12. 2008
    ....
  53. ncbi request reprint Quantification of intracellular homocysteine by stable isotope dilution liquid chromatography/tandem mass spectrometry
    Yuehua Huang
    Centers for Cancer Pharmacology and Pharmacogenetics, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6160, USA
    Biomed Chromatogr 21:107-12. 2007
    ..The limit of detection was 2 ng/10(6) EA.hy 926 cells. Using this method, the intracellular homocysteine concentration in EA.hy 926 cells ranged from 10 to 36 ng/10(6) cells...
  54. ncbi request reprint Targeted quantitative analysis of fatty acids in atherosclerotic plaques by high sensitivity liquid chromatography/tandem mass spectrometry
    Caterina Pettinella
    Center for Cancer Pharmacology, University of Pennsylvania School of Medicine, 854 BRB II III, 421 Curie Boulevard, Philadelphia, PA 19104 6160, USA
    J Chromatogr B Analyt Technol Biomed Life Sci 850:168-76. 2007
    ..The method was validated, had a detection limit in the fmol range, and was used in the analysis of fatty acids in atherosclerotic plaques from carotid arteries...
  55. ncbi request reprint Aldo-keto reductase- and cytochrome P450-dependent formation of benzo[a]pyrene-derived DNA adducts in human bronchoalveolar cells
    Qian Ruan
    Centers for Cancer Pharmacology and Excellence in Environmental Toxicology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 6160, USA
    Chem Res Toxicol 20:424-31. 2007
    ..These data raise the intriguing possibility that P450 1A1/P450 1B1 and AKR1A1 may be protective against (+)-B[a]PDE-mediated DNA damage...
  56. ncbi request reprint Liquid chromatography/tandem mass spectrometry characterization of oxidized amyloid beta peptides as potential biomarkers of Alzheimer's disease
    Koichi Inoue
    Center for Cancer Pharmacology, University of Pennsylvania School of Medicine, 854 BRB II III, 421 Curie Boulevard, Philadelphia, PA 19104 6160, USA
    Rapid Commun Mass Spectrom 20:911-8. 2006
    ..Using methodology based on LC/MS/MS it will also be possible to analyze the relative amounts of oxidized peptides and native peptide in cerebrospinal fluid from patients with Alzheimer's disease as biomarkers of oxidative stress...
  57. pmc Metabolism of benzo[a]pyrene in human bronchoalveolar H358 cells using liquid chromatography-mass spectrometry
    Hao Jiang
    Centers of Excellence in Environmental Toxicology and Cancer Pharmacology, Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6084, USA
    Chem Res Toxicol 20:1331-41. 2007
    ..It also provides evidence that each of the pathways of PAH activation yields their distinctive metabolites in H358 human lung cells and that each pathway may contribute to the carcinogenic process...
  58. ncbi request reprint Cyclooxygenase-2-mediated metabolism of arachidonic acid to 15-oxo-eicosatetraenoic acid by rat intestinal epithelial cells
    Seon Hwa Lee
    Center for Cancer Pharmacology, University of Pennsylvania, 854 BRB II III, 421 Curie Boulevard, Philadelphia, Pennsylvania 19104 6160, USA
    Chem Res Toxicol 20:1665-75. 2007
    ..These data further highlight the importance of endogenous COX-2-mediated lipid peroxidation and illustrate the necessity to monitor eicosanoid formation from endogenous stores of AA in cell culture experiments...
  59. ncbi request reprint Targeted chiral lipidomics analysis by liquid chromatography electron capture atmospheric pressure chemical ionization mass spectrometry (LC-ECAPCI/MS)
    Seon Hwa Lee
    Centers for Cancer Pharmacology and Excellence in Environmental Toxicology, University of Pennsylvania, Philadelphia, Pennsylvania, USA
    Methods Enzymol 433:159-74. 2007
    ..A combination of stable isotope dilution methodology and chiral liquid chromatography-ECAPCI/MS makes it possible to resolve and quantify complex mixtures of regioisomeric and enantiomeric oxidized lipids...
  60. pmc Analysis of the zebrafish proteome during embryonic development
    Margaret B Lucitt
    Institute for Translational Medicine and Therapeutics, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Mol Cell Proteomics 7:981-94. 2008
    ..This new resource should facilitate the selection of candidate proteins for targeted quantitation and refine systematic genetic network analysis in vertebrate development and biology...
  61. ncbi request reprint Unexpected formation of etheno-2'-deoxyguanosine adducts from 5(S)-hydroperoxyeicosatetraenoic acid: evidence for a bis-hydroperoxide intermediate
    Wenying Jian
    Center for Cancer Pharmacology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 6160, USA
    Chem Res Toxicol 18:599-610. 2005
    ..The less abundant carboxylate adduct IV arose from a quite different pathway and was tentatively characterized as 6-carboxy-3-hydroxy-1-hexene-1,N2-etheno-2'-deoxyguanosine...
  62. ncbi request reprint Determination of the platinum drug cis-amminedichloro(2-methylpyridine)platinum(II) in human urine by liquid chromatography-tandem mass spectrometry
    Tomoyuki Oe
    Center for Cancer Pharmacology, Department of Pharmacology, University of Pennsylvania, 1254 BRB II III, 421 Curie Boulevard, Philadelphia, PA 19104 6160, USA
    J Chromatogr B Analyt Technol Biomed Life Sci 792:217-27. 2003
    ..Formation of the adduct was prevented by the addition of 50% (w/v) sodium chloride to the urine. The assay can be used to quantify intact ZD0473 in the urine of subjects dosed with this new platinum drug...
  63. ncbi request reprint DNA topoisomerase II in therapy-related acute promyelocytic leukemia
    Anita R Mistry
    Department of Medical and Molecular Genetics, Guy s, King s, and St Thomas School of Medicine, London
    N Engl J Med 352:1529-38. 2005
    ..We studied acute promyelocytic leukemia (APL) with the t(15;17) translocation that developed after treatment of breast or laryngeal cancer with chemotherapeutic agents that poison topoisomerase II...
  64. ncbi request reprint Convenient and efficient syntheses of 4-hydroxy-2(E)-nonenal and 4-oxo-2(E)-nonenal
    Reshma F Kurangi
    Department of Chemistry, Goa University, Taliegao Plateau, Goa, India
    Lipids 41:877-80. 2006
    ..Hydrolysis gave 4-oxo-2(E)-nonenal, whereas reduction followed by hydrolysis gave 4-hydroxy-2(E)-nonenal...
  65. pmc Interaction with phospholipids modulates alpha-synuclein nitration and lipid-protein adduct formation
    Andrés Trostchansky
    Center for Free Radical and Biomedical Research, Departamento de Bioquimica, Facultad de Medicina, Universidad de la Republica, Avenida General Flores 2125, CP 11800, Montevideo, Uruguay
    Biochem J 393:343-9. 2006
    ....

Research Grants8

  1. Symposium on Proteomics and Toxicology
    Ian Blair; Fiscal Year: 2003
    ..It is anticipated that the symposium will also provide the opportunity to introduce new investigators to the field. ..
  2. Acquisition of a linear Q-Trap LC/MS/MS System
    Ian Blair; Fiscal Year: 2004
    ..Therefore, the new instrument would impact significantly on research programs of some 38-48 NIH-funded investigators at the University of Pennsylvania. ..
  3. Fourier Transform Mass Spec Proteomics and Genomics
    Ian Blair; Fiscal Year: 2004
    ..Thus, while each investigator's research proposal stands alone in scientific value and integrity, the added value of this combined proposal is substantial. ..
  4. High resolution triple quadrupole mass spectrometer
    Ian Blair; Fiscal Year: 2005
    ..Therefore, the new instrument would impact significantly on research programs of some 40-50 NIH-funded investigators at the University of Pennsylvania. ..
  5. TRAINING PROGRAM IN CANCER PHARMACOLOGY
    Ian Blair; Fiscal Year: 2007
    ..Adamson). Both of these mentors have research programs with a focus on translational therapeutics and so they provide another outstanding training resource. ..
  6. Molecular mechanisms of DNA damage by PAHs
    Ian Blair; Fiscal Year: 2007
    ..Successful completion of the proposed research will permit provide a new approach and novel methodology for determining the inter-individual risk factors for DNA damage, which result from environmental exposure to B[a]P. ..
  7. Exposure and biological response biomarkers of cigarette smoke
    Ian Blair; Fiscal Year: 2007
    ....