Research Topics
| Douglas BlackSummaryAffiliation: University of California Country: USA Publications
Research Grants
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Detail Information
Publications
Roles for SR proteins and hnRNP A1 in the regulation of c-src exon N1Nanette Rooke
Department of Microbiology, Immunology and Molecular Genetics, University of California Los Angeles, 1602 Molecular Sciences Building, 405 Hilgard Avenue, Los Angeles, CA 90095, USA
Mol Cell Biol 23:1874-84. 2003..Our results link the activity of these well-known exonic splicing regulators, SF2/ASF and hnRNP A1, to the splicing of an exon primarily controlled by intronic factors...- To cross or not to cross: alternatively spliced forms of the Robo3 receptor regulate discrete steps in axonal midline crossingDouglas L Black
Department of Microbiology, Immunology and Molecular Genetics, Howard Hughes Medical Institute, David Geffen School of Medicine, MacDonald Research Laboratories, 675 Charles E Young Drive South, Los Angeles, CA 90095, USA
Neuron 58:297-8. 2008.... 
Splicing bioinformatics to biologyDouglas L Black
Department of Microbiology, Immunology and Molecular Genetics, Howard Hughes Medical Institute, University of California, Los Angeles, CA 90095-1662, USA
Genome Biol 7:317. 2006- A simple answer for a splicing conundrumDouglas L Black
Howard Hughes Medical Institute, University of California, Los Angeles, CA 90095-1662, USA
Proc Natl Acad Sci U S A 102:4927-8. 2005 
Mechanisms of alternative pre-messenger RNA splicingDouglas L Black
Department of Microbiology, Immunology, and Molecular Genetics, Howard Hughes Medical Institute, University of California Los Angeles, Los Angeles, California 90095 1662, USA
Annu Rev Biochem 72:291-336. 2003..How these regulators are combined into complex systems of tissue-specific splicing is discussed. In conclusion, very recent studies are presented that point to new directions for understanding alternative splicing and its mechanisms...- Maps, codes, and sequence elements: can we predict the protein output from an alternatively spliced locus?Shalini Sharma
Department of Microbiology, Immunology, and Molecular Genetics, Howard Hughes Medical Institute, University of California, Los Angeles, Los Angeles, California 90095, USA
Neuron 52:574-6. 2006..A recent study describes how for a particular neuronal splicing regulatory protein, Nova, the location of its binding sites is highly predictive of the protein's effect on an exon's splicing... - Depolarization and CaM kinase IV modulate NMDA receptor splicing through two essential RNA elementsJi Ann Lee
Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, Los Angeles, California, United States of America
PLoS Biol 5:e40. 2007..Many of these exons are likely to alter neuronal function. Thus, these two RNA elements define a group of co-regulated splicing events that respond to a common stimulus in neurons to alter their activity... - Polypyrimidine tract binding protein controls the transition from exon definition to an intron defined spliceosomeShalini Sharma
Howard Hughes Medical Institute, University of California, Los Angeles, MRL5 748, Charles E Young Drive South, Los Angeles, California 90095, USA
Nat Struct Mol Biol 15:183-91. 2008..Proteomic analyses of these complexes provide a new description of exon definition complexes, and indicate that splicing regulators can act on the transition between the exon definition complex and an intron-defined spliceosome... - Exon repression by polypyrimidine tract binding proteinBatoul Amir-Ahmady
Department of Microbiology, Immunology and Molecular Genetics, University of California Los Angeles, Los Angeles, CA 90095-1662, USA
RNA 11:699-716. 2005..The minimal complex mediating splicing repression by PTB requires two binding sites bound by an oligomeric PTB complex... - Co-transcriptional splicing of constitutive and alternative exonsAmy Pandya-Jones
Department of Microbiology, Immunology and Molecular Genetics, University of California at Los Angeles, Los Angeles, California 90095, USA
RNA 15:1896-908. 2009..Thus, we demonstrate that the decision to include or skip an alternative exon is made during transcription and not post-transcriptionally... - Homologues of the Caenorhabditis elegans Fox-1 protein are neuronal splicing regulators in mammalsJason G Underwood
Molecular Biology Institute, University of California, Los Angeles, Los Angeles, California 90095 1662, USA
Mol Cell Biol 25:10005-16. 2005..They do this through a splicing enhancer function, in addition to their apparent negative effects on splicing in vertebrate muscle and in worms... - Sam68 regulates a set of alternatively spliced exons during neurogenesisGeetanjali Chawla
Howard Hughes Medical Institute, University of California, Los Angeles, 6 762 MacDonald Research Laboratories, 675 Charles E Young Drive South, Los Angeles, California 90095 1662, USA
Mol Cell Biol 29:201-13. 2009..Thus, Sam68 controls neurogenesis through its effects on a specific set of RNA targets... - A post-transcriptional regulatory switch in polypyrimidine tract-binding proteins reprograms alternative splicing in developing neuronsPaul L Boutz
Department of Microbiology, Immunology, and Molecular Genetics, 6 762 MacDonald Research Laboratories, Los Angeles, CA 90095, USA
Genes Dev 21:1636-52. 2007..These data show that the post-transcriptional switch from PTB to nPTB controls a widespread alternative splicing program during neuronal development... - A consensus CaMK IV-responsive RNA sequence mediates regulation of alternative exons in neuronsJiuyong Xie
Department of Microbiology, Immunology and Molecular Genetics, University of California Los Angeles, Los Angeles, CA 90095-1662, USA
RNA 11:1825-34. 2005..The functions of some of these exons imply that splicing control through the CaMK IV pathway will alter neuronal activity... - Polypyrimidine tract binding protein blocks the 5' splice site-dependent assembly of U2AF and the prespliceosomal E complexShalini Sharma
Howard Hughes Medical Institute, University of California, Los Angeles, MRL5-748, Charles E. Young Drive South, Los Angeles, California 90095, USA
Mol Cell 19:485-96. 2005..Thus, rather than directly blocking the N1 splice sites, PTB prevents the 5' splice site-dependent assembly of U2AF into the E complex. This mechanism likely occurs in many other alternative exons... - Differentiation-induced colocalization of the KH-type splicing regulatory protein with polypyrimidine tract binding protein and the c-src pre-mRNAMegan P Hall
Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, Los Angeles, California 90095, USA
Mol Biol Cell 15:774-86. 2004..These results indicate that PTB and KSRP do indeed interact with the c-src transcript in vivo, and that these associations change with the differentiated state of the cell... - An inducible change in Fox-1/A2BP1 splicing modulates the alternative splicing of downstream neuronal target exonsJi Ann Lee
Department of Microbiology, Immunology, and Molecular Genetics, University of California at Los Angeles, Los Angeles, California 90095, USA
Genes Dev 23:2284-93. 2009..These results reveal a novel mechanism for the slow modulation of splicing as cells adapt to chronic stimuli: The subcellular localization of a splicing regulator is controlled through its own alternative splicing... - Developmental control of CaV1.2 L-type calcium channel splicing by Fox proteinsZhen Zhi Tang
Howard Hughes Medical Institute, University of California at Los Angeles, Los Angeles, California 90095 1662, USA
Mol Cell Biol 29:4757-65. 2009..These results demonstrate that the Fox protein family is playing a key role in tuning the properties of CaV1.2 calcium channels during neuronal development... - A high-throughput screening strategy identifies cardiotonic steroids as alternative splicing modulatorsPeter Stoilov
Department of Microbiology, Immunology, and Molecular Genetics and Howard Hughes Medical Institute, University of California, Los Angeles, CA 90095 1662, USA
Proc Natl Acad Sci U S A 105:11218-23. 2008..Our results identify currently prescribed cardiotonic steroids as modulators of alternative splicing and demonstrate the feasibility of screening for drugs that alter exon inclusion... - Neuronal regulation of alternative pre-mRNA splicingQin Li
Howard Hughes Medical Institute, University of California, Los Angeles, 6 762 MacDonald Research Laboratories, 675 Charles E Young Drive South, Los Angeles, California 90095, USA
Nat Rev Neurosci 8:819-31. 2007..Finally, we describe regulatory proteins that control the splicing of some neuronally expressed transcripts... - MicroRNAs regulate the expression of the alternative splicing factor nPTB during muscle developmentPaul L Boutz
Department of Microbiology, Immunology, and Molecular Genetics, University of California at Los Angeles, Los Angeles, California 90095, USA
Genes Dev 21:71-84. 2007..These results indicate that miR-133 directly down-regulates a key splicing factor during muscle development and establishes a role for microRNAs in the control of a developmentally dynamic splicing program... - Autoregulation of Fox protein expression to produce dominant negative splicing factorsAndrey Damianov
Howard Hughes Medical Institute, University of California at Los Angeles, Los Angeles, California 90095, USA
RNA 16:405-16. 2010..Thus, rather than autoregulation of splicing controlling the abundance of the regulator, the Fox proteins use a highly conserved mechanism of splicing autoregulation to control production of a dominant negative isoform... - Protein kinase A phosphorylation modulates transport of the polypyrimidine tract-binding proteinJiuyong Xie
Howard Hughes Medical Institute, Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, CA 90095 1662, USA
Proc Natl Acad Sci U S A 100:8776-81. 2003..Thus, direct PKA phosphorylation of PTB at Ser-16 modulates the nucleo-cytoplasmic distribution of PTB. This phosphorylation likely plays a role in the cytoplasmic function of PTB... - Structure of PTB bound to RNA: specific binding and implications for splicing regulationFlorian C Oberstrass
Institute for Molecular Biology and Biophysics, Department of Biology, Swiss Federal Institute of Technology, Zurich, ETH Honggerberg, CH 8093 Zurich, Switzerland
Science 309:2054-7. 2005..Thus, PTB will induce RNA looping when bound to two separated pyrimidine tracts within the same RNA. This leads to structural models for how PTB functions as an alternative-splicing repressor... - MADS: a new and improved method for analysis of differential alternative splicing by exon-tiling microarraysYi Xing
Department of Internal Medicine, University of Iowa, Iowa City, Iowa 52242, USA
RNA 14:1470-9. 2008..Thirty novel events were tested by RT-PCR and 27 were confirmed. This work demonstrates that the exon-tiling microarray design is an efficient and powerful approach for global, unbiased analysis of pre-mRNA splicing... - Molecular basis of RNA recognition by the human alternative splicing factor Fox-1Sigrid D Auweter
Institute for Molecular Biology and Biophysics, Swiss Federal Institute of Technology ETH Zurich, Zurich, Switzerland
EMBO J 25:163-73. 2006..These results demonstrate the unusual molecular mechanism of sequence-specific RNA recognition by Fox-1, which is exceptional in its high affinity for a defined but short sequence element...
Research Grants
- Genomic Measurement of Alternative Splicing by DNA ArrayDouglas Black; Fiscal Year: 2007..abstract_text> ..
- The Regulation of Neuronal Exon SplicingDouglas L Black; Fiscal Year: 2010..For these diseases to be approached therapeutically, much more information is needed on the mechanisms of splicing regulation and its role in neuronal function. ..
- The Regulation of Neuronal Exon SplicingDouglas Black; Fiscal Year: 2009..For these diseases to be approached therapeutically, much more information is needed on the mechanisms of splicing regulation and its role in neuronal function. ..
- REGULATION OF C-SRC PREMRNA SPLICINGDouglas Black; Fiscal Year: 2006..From this work we hope to understand in precise molecular detail how a variety of combinatorial inputs can determine a change in splicing. ..
- The Regulation of Neuronal Exon SplicingDouglas Black; Fiscal Year: 2007..For these diseases to be approached therapeutically, much more information is needed on the mechanisms of splicing regulation and its role in neuronal function. ..
- REGULATION OF C-SRC PREMRNA SPLICINGDouglas Black; Fiscal Year: 2001..Finally, simplified splicing systems will be developed for the analysis of individual regulatory proteins. Our goal is to understand in molecular detail how a simple change in splicing pattern is regulated in differentiated cells. ..
- The Regulation of Neuronal Exon SplicingDouglas Black; Fiscal Year: 2009..For these diseases to be approached therapeutically, much more information is needed on the mechanisms of splicing regulation and its role in neuronal function. ..