Research Topics
| LEONARD BJELDANESSummaryAffiliation: University of California Country: USA Publications
Research Grants
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Detail Information
Publications
Plant-derived 3,3'-Diindolylmethane is a strong androgen antagonist in human prostate cancer cellsHien T Le
Department of Nutritional Sciences and Toxicology, The University of California, Berkeley, California 94720 3104, USA
J Biol Chem 278:21136-45. 2003..To our knowledge, DIM is the first example of a pure androgen receptor antagonist from plants...- Indole-3-carbinol induces a G1 cell cycle arrest and inhibits prostate-specific antigen production in human LNCaP prostate carcinoma cellsJoann Zhang
Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720-3200, USA
Cancer 98:2511-20. 2003..These findings implicate this dietary indole as a potential chemotherapeutic agent for controlling the growth of human prostate carcinoma cells... - 3,3'-Diindolylmethane (DIM) induces a G(1) cell cycle arrest in human breast cancer cells that is accompanied by Sp1-mediated activation of p21(WAF1/CIP1) expressionChibo Hong
Department of Nutritional Sciences and Toxicology, University of California, Berkeley, CA 94720, USA
Carcinogenesis 23:1297-305. 2002..Thus, our observations have uncovered an antiproliferative pathway for DIM that implicates Sp1/Sp3-induced expression of p21 as a target for cell cycle control in human breast cancer cells... - Activation and potentiation of interferon-gamma signaling by 3,3'-diindolylmethane in MCF-7 breast cancer cellsJacques E Riby
Department of Nutritional Sciences and Toxicology, University of California, Berkeley, 94720-3104, USA
Mol Pharmacol 69:430-9. 2006..These results reveal novel immune activating and potentiating activities of DIM in human tumor cells that may contribute to the established effectiveness of this dietary indole against various tumors types... - Potent ligand-independent estrogen receptor activation by 3,3'-diindolylmethane is mediated by cross talk between the protein kinase A and mitogen-activated protein kinase signaling pathwaysHoyee Leong
Department of Nutritional Sciences and Toxicology, University of California, Berkeley, California 94720, USA
Mol Endocrinol 18:291-302. 2004..These results demonstrate that DIM is a mechanistically novel activator of the ER that requires PKA-dependent phosphorylation of CREB... - Fate of indole-3-carbinol in cultured human breast tumor cellsRichard E Staub
Department of Nutritional Sciences and Toxicology, University of California, Berkeley, California 94720, USA
Chem Res Toxicol 15:101-9. 2002..The accumulation of DIM in the nucleus suggests that this product may have a role in the cellular biological activities of I3C.3.. - Indole-3-carbinol and 3-3'-diindolylmethane antiproliferative signaling pathways control cell-cycle gene transcription in human breast cancer cells by regulating promoter-Sp1 transcription factor interactionsGary L Firestone
Department of Molecular and Cell Biology and Cancer Research Laboratory, University of California at Berkeley, Berkeley, CA 94720 3200, USA
J Nutr 133:2448S-2455S. 2003.... - Indole-3-carbinol mediated cell cycle arrest of LNCaP human prostate cancer cells requires the induced production of activated p53 tumor suppressor proteinJocelyn C Hsu
Department of Molecular and Cell Biology and The Cancer Research Laboratory, The University of California at Berkeley, Berkeley, CA 94720, USA
Biochem Pharmacol 72:1714-23. 2006..Taken together, our results establish that the I3C induced G1 arrest of human prostate cancer cells requires the induced production of the activated phosphorylated forms of p53, which stimulate transcription of the CDK2 inhibitor p21... - Bcl-2 family-mediated apoptotic effects of 3,3'-diindolylmethane (DIM) in human breast cancer cellsChibo Hong
Department of Nutritional Sciences and Toxicology, University of California, Berkeley 94720-3200, USA
Biochem Pharmacol 63:1085-97. 2002..These results demonstrate that DIM can induce apoptosis in breast cancer cells independent of estrogen receptor status by a process that is mediated by the modulated expression of the Bax/Bcl-2 family of apoptotic regulatory factors... - Fate of 3,3'-diindolylmethane in cultured MCF-7 human breast cancer cellsRichard E Staub
Department of Nutritional Sciences and Toxicology, University of California, Berkeley, California 94720, USA
Chem Res Toxicol 19:436-42. 2006..These findings show that the uptake of DIM by membranes significantly increases its localized concentration, which may contribute to its biological activities... 
Direct inhibition of elastase activity by indole-3-carbinol triggers a CD40-TRAF regulatory cascade that disrupts NF-kappaB transcriptional activity in human breast cancer cellsIda Aronchik
Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720 3200, USA
Cancer Res 70:4961-71. 2010..Furthermore, we have established a new I3C-mediated antiproliferative cascade that has significant therapeutic potential for treatment of human cancers associated with high levels of elastase and its CD40 membrane substrate...- Indole-3-carbinol activates the ATM signaling pathway independent of DNA damage to stabilize p53 and induce G1 arrest of human mammary epithelial cellsChristine T Brew
Department of Molecular and Cell Biology and The Cancer Research Laboratory, University of California at Berkeley, Berkeley, CA 94720-3200, USA
Int J Cancer 118:857-68. 2006.... - DIM stimulates IFNgamma gene expression in human breast cancer cells via the specific activation of JNK and p38 pathwaysLing Xue
Department of Nutritional Sciences and Toxicology, University of California, 119 Morgan Hall, Berkeley, CA 94720-3104, USA
Oncogene 24:2343-53. 2005..These results establish that DIM-induced IFNgamma expression in human breast tumor cells is mediated by activation of both JNK and p38 pathways, which is ultimately dependent on intracellular calcium signaling... - 3,3'-Diindolylmethane inhibits angiogenesis and the growth of transplantable human breast carcinoma in athymic miceXiaofei Chang
Department of Nutritional Sciences and Toxicology, University of California, Berkeley, CA 94720, USA
Carcinogenesis 26:771-8. 2005..This is the first study to show that DIM can strongly inhibit the development of human breast tumor in a xenograft model and to provide evidence for the antiangiogenic properties of this dietary indole... - Indole-3-carbinol stimulates transcription of the interferon gamma receptor 1 gene and augments interferon responsiveness in human breast cancer cellsUrmi Chatterji
Department of Molecular and Cell Biology and The Cancer Research Laboratory, University of California at Berkeley, Berkeley, CA 94720-3200, USA
Carcinogenesis 25:1119-28. 2004..Our results suggest that one mechanism by which I3C mediates these anticancer effects is by stimulating expression of the IFNgammaR1 and augmenting the IFNgamma response in MCF-7 human breast cancer cells... - Effects of analogs of indole-3-carbinol cyclic trimerization product in human breast cancer cellsLing Xue
Department of Nutritional Sciences and Toxicology, 119 Morgan Hall, University of California, Berkeley, CA 94720, USA
Chem Biol Interact 152:119-29. 2005.... 
3,3'-Diindolylmethane induces a G(1) arrest in human prostate cancer cells irrespective of androgen receptor and p53 statusOmar I Vivar
Department of Nutritional Sciences and Toxicology, 119 Morgan Hall, University of California, Berkeley, CA 94720, USA
Biochem Pharmacol 78:469-76. 2009..The Sp1 and p38 MAPK pathways mediate the DIM cell cycle regulatory effect in DU145 cells...- The dietary phytochemical indole-3-carbinol is a natural elastase enzymatic inhibitor that disrupts cyclin E protein processingHanh H Nguyen
Department of Molecular and Cell Biology and Cancer Research Laboratory, University of California, Berkeley, CA 94720, USA
Proc Natl Acad Sci U S A 105:19750-5. 2008.... - 3,3'-diindolylmethane reduces levels of HIF-1alpha and HIF-1 activity in hypoxic cultured human cancer cellsJacques E Riby
Department of Nutritional Sciences and Toxicology, 217 Morgan Hall, University of California, Berkeley, CA 94720, USA
Biochem Pharmacol 75:1858-67. 2008..These studies are the first to show that DIM can decrease the accumulation and activity of the key angiogenesis regulatory factor, HIF-1alpha, in hypoxic tumor cells... - N-Alkoxy derivatization of indole-3-carbinol increases the efficacy of the G1 cell cycle arrest and of I3C-specific regulation of cell cycle gene transcription and activity in human breast cancer cellsSarah M Jump
Department of Molecular and Cell Biology and The Cancer Research Laboratory, University of California at Berkeley, Berkeley, CA 94720 3200, USA
Biochem Pharmacol 75:713-24. 2008.... - Inhibition of growth factor-induced Ras signaling in vascular endothelial cells and angiogenesis by 3,3'-diindolylmethaneXiaofei Chang
Department of Nutritional Sciences and Toxicology, University of California, Berkeley, CA 94720, USA
Carcinogenesis 27:541-50. 2006..These data provide evidence that DIM inhibits Ras signaling induced by VEGF and other growth factors, which interferes with its downstream biological effects necessary for angiogenesis... - 3,3'-Diindolylmethane is a novel mitochondrial H(+)-ATP synthase inhibitor that can induce p21(Cip1/Waf1) expression by induction of oxidative stress in human breast cancer cellsYixuan Gong
Department of Nutritional Sciences and Toxicology, University of California, Berkeley, California 94720, USA
Cancer Res 66:4880-7. 2006..Thus, we have established the critical role of enhanced mitochondrial ROS release in 3,3'-diindolylmethane-induced p21 up-regulation in human breast cancer cells... - Indole-3-carbinol (I3C) inhibits cyclin-dependent kinase-2 function in human breast cancer cells by regulating the size distribution, associated cyclin E forms, and subcellular localization of the CDK2 protein complexHanh H Garcia
Department of Molecular and Cell Biology and The Cancer Research Laboratory, The University of California at Berkeley, Berkeley, California 94720, USA
J Biol Chem 280:8756-64. 2005.... - 3,3'-Diindolylmethane stimulates murine immune function in vitro and in vivoLing Xue
Department of Nutritional Sciences and Toxicology, University of California, Berkeley, CA 94720 3104, USA
J Nutr Biochem 19:336-44. 2008..Thus, DIM is a potent stimulator of immune function. This property might contribute to the cancer inhibitory effects of this indole... - Pilot study: effect of 3,3'-diindolylmethane supplements on urinary hormone metabolites in postmenopausal women with a history of early-stage breast cancerKathie M Dalessandri
Department of Molecular and Cell Biology, University of California, Berkeley, 94720-3200, USA
Nutr Cancer 50:161-7. 2004..020), DIM (P =0. 045), and cortisol (P = 0.039), and a nonsignificant increase of 47% in the 2-OHE1/16alpha-OHE1 ratio from 1.46 to 2.14 (P=0.059). In this pilot study, DIM increased the 2-hydroxylation of estrogen urinary metabolites... - Selective activation of estrogen receptor-beta target genes by 3,3'-diindolylmethaneOmar I Vivar
Department of Nutritional Science and Toxicology, University of California, Berkeley, Berkeley, California 94720 3104, USA
Endocrinology 151:1662-7. 2010..Our results demonstrate that DIM is a new class of ERbeta-selective compounds, because it does not bind to ERbeta, but instead it selectively recruits ERbeta and coactivators to target genes... - Indole-3-carbinol inhibits MDA-MB-231 breast cancer cell motility and induces stress fibers and focal adhesion formation by activation of Rho kinase activityChristine T Brew
Department of Molecular and Cell Biology, The University of California at Berkeley, Berkeley, CA 94720 3200, USA
Int J Cancer 124:2294-302. 2009..Taken together, our data demonstrate that I3C induces stress fibers and peripheral focal adhesions in a Rho kinase-dependent manner that leads to an inhibition of motility in human breast cancer cells... - Convenient synthesis of 5,6,11,12,17,18- hexahydrocyclononal[1,2-b:4,5-b':7,8-b'']triindole, a novel phytoestrogenRichard E Staub
Department of Nutritional Sciences and Toxicology, University of California, Berkeley, California 94720-3104, USA
J Org Chem 68:167-9. 2003..For the procedure, gramine is treated with dimethyl sulfate and sodium in ethanol at room temperature. Quenching of the reaction with water and workup of the product provides CTr in approximately 75% yield... - 3,3'-diindolylmethane is a novel topoisomerase IIalpha catalytic inhibitor that induces S-phase retardation and mitotic delay in human hepatoma HepG2 cellsYixuan Gong
Department of Nutritional Sciences and Toxicology, 119 Morgan Hall, University of California, Berkeley, CA 94720-3104, USA
Mol Pharmacol 69:1320-7. 2006..Our results identified a new mode of action for this intriguing dietary component that might be exploited for therapeutic development... - Indole-3-carbinol inhibition of androgen receptor expression and downregulation of androgen responsiveness in human prostate cancer cellsJocelyn C Hsu
Department of Molecular and Cell Biology, The University of California at Berkeley, CA 94720, USA
Carcinogenesis 26:1896-904. 2005..Taken together, our results demonstrate that I3C represses AR expression and responsiveness in LNCaP cells as a part of its antiproliferative mechanism... - Indole-3-carbinol selectively uncouples expression and activity of estrogen receptor subtypes in human breast cancer cellsShyam N Sundar
Department of Molecular and Cell Biology, 591 LSA, University of California at Berkeley, Berkeley, California 94720-3200 USA
Mol Endocrinol 20:3070-82. 2006.... - Liquiritigenin is a plant-derived highly selective estrogen receptor beta agonistJennifer E Mersereau
Department of Obstetrics, Gynecology and Reproductive Sciences and Center for Reproductive Sciences, Cellular and Molecular Pharmacology, University of California, San Francisco, CA, United States
Mol Cell Endocrinol 283:49-57. 2008..In a mouse xenograph model, liquiritigenin did not stimulate uterine size or tumorigenesis of MCF-7 breast cancer cells. Our results demonstrate that some plants contain highly selective estrogens for ERbeta...
Research Grants
- DIETARY INDOLES AS BREAST TUMOR CELL GROWTH INHIBITORSLEONARD BJELDANES; Fiscal Year: 1999..To determine if the growth inhibitory effects of the I3C conversion products depend on AhR, AhR antisense blocking experiments will be conducted. ..