LEONARD BJELDANES

Summary

Affiliation: University of California
Country: USA

Publications

  1. ncbi request reprint Plant-derived 3,3'-Diindolylmethane is a strong androgen antagonist in human prostate cancer cells
    Hien T Le
    Department of Nutritional Sciences and Toxicology, The University of California, Berkeley, California 94720 3104, USA
    J Biol Chem 278:21136-45. 2003
  2. ncbi request reprint Indole-3-carbinol induces a G1 cell cycle arrest and inhibits prostate-specific antigen production in human LNCaP prostate carcinoma cells
    Joann Zhang
    Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720 3200, USA
    Cancer 98:2511-20. 2003
  3. ncbi request reprint 3,3'-Diindolylmethane (DIM) induces a G(1) cell cycle arrest in human breast cancer cells that is accompanied by Sp1-mediated activation of p21(WAF1/CIP1) expression
    Chibo Hong
    Department of Nutritional Sciences and Toxicology, University of California, Berkeley, CA 94720, USA
    Carcinogenesis 23:1297-305. 2002
  4. ncbi request reprint Activation and potentiation of interferon-gamma signaling by 3,3'-diindolylmethane in MCF-7 breast cancer cells
    Jacques E Riby
    Department of Nutritional Sciences and Toxicology, University of California, Berkeley, 94720 3104, USA
    Mol Pharmacol 69:430-9. 2006
  5. ncbi request reprint Potent ligand-independent estrogen receptor activation by 3,3'-diindolylmethane is mediated by cross talk between the protein kinase A and mitogen-activated protein kinase signaling pathways
    Hoyee Leong
    Department of Nutritional Sciences and Toxicology, University of California, Berkeley, California 94720, USA
    Mol Endocrinol 18:291-302. 2004
  6. ncbi request reprint Fate of indole-3-carbinol in cultured human breast tumor cells
    Richard E Staub
    Department of Nutritional Sciences and Toxicology, University of California, Berkeley, California 94720, USA
    Chem Res Toxicol 15:101-9. 2002
  7. ncbi request reprint Indole-3-carbinol and 3-3'-diindolylmethane antiproliferative signaling pathways control cell-cycle gene transcription in human breast cancer cells by regulating promoter-Sp1 transcription factor interactions
    Gary L Firestone
    Department of Molecular and Cell Biology and Cancer Research Laboratory, University of California at Berkeley, Berkeley, CA 94720 3200, USA
    J Nutr 133:2448S-2455S. 2003
  8. ncbi request reprint Indole-3-carbinol mediated cell cycle arrest of LNCaP human prostate cancer cells requires the induced production of activated p53 tumor suppressor protein
    Jocelyn C Hsu
    Department of Molecular and Cell Biology and The Cancer Research Laboratory, The University of California at Berkeley, Berkeley, CA 94720, USA
    Biochem Pharmacol 72:1714-23. 2006
  9. ncbi request reprint Bcl-2 family-mediated apoptotic effects of 3,3'-diindolylmethane (DIM) in human breast cancer cells
    Chibo Hong
    Department of Nutritional Sciences and Toxicology, University of California, Berkeley 94720 3200, USA
    Biochem Pharmacol 63:1085-97. 2002
  10. ncbi request reprint Fate of 3,3'-diindolylmethane in cultured MCF-7 human breast cancer cells
    Richard E Staub
    Department of Nutritional Sciences and Toxicology, University of California, Berkeley, California 94720, USA
    Chem Res Toxicol 19:436-42. 2006

Research Grants

  1. DIETARY INDOLES AS BREAST TUMOR CELL GROWTH INHIBITORS
    LEONARD BJELDANES; Fiscal Year: 1999

Collaborators

  • GARY FIRESTONE
  • H Leon Bradlow
  • Maria N Preobrazhenskaya
  • Dale C Leitman
  • WILLIAM RICKE
  • Ling Xue
  • Jacques E Riby
  • Richard E Staub
  • Ida Aronchik
  • Christine T Brew
  • Jocelyn C Hsu
  • Omar I Vivar
  • Yixuan Gong
  • Chibo Hong
  • Xiaofei Chang
  • Urmi Chatterji
  • Joann Zhang
  • Jennifer E Mersereau
  • Hanh H Nguyen
  • Sarah M Jump
  • Gloria A Brar
  • David H H Nguyen
  • Shyam N Sundar
  • Anurupa Dev
  • Aimee Wing
  • Erik L Bjeldanes
  • Hanh H Garcia
  • Bruce Onisko
  • Hoyee Leong
  • Charlene M Schaldach
  • Kathie M Dalessandri
  • Hyeon A Kim
  • Hien T Le
  • Elise F Saunier
  • Karena Kosco
  • Chia Lei Lin
  • Jasmine McCammon
  • Maoxiang Li
  • Isaac Cohen
  • Tetjana Zogric
  • James J Pestka
  • Mary Tagliaferri
  • Sylvia Chow
  • Scott Baggett
  • Jenny Kung
  • Tatjana Zogovic
  • Erin J Cram
  • Nitzan Levy
  • Larisa N Yudina
  • Matthew A Kinseth
  • Richard Staub
  • Heesook Sohn
  • Vaishali Kerekatte
  • Robert B Dickson
  • Victor B Doan
  • Joon Ho Sheen
  • Caterina N Equinozio
  • Jan Bergman
  • Janet C Tou
  • Tomasz Janosik
  • Mark D Fitch
  • Taketoshi Taniguchi
  • Matthew A Kinseth B A
  • Jocelyn C Hsu B A
  • Chunling Feng
  • George S Bailey

Detail Information

Publications32

  1. ncbi request reprint Plant-derived 3,3'-Diindolylmethane is a strong androgen antagonist in human prostate cancer cells
    Hien T Le
    Department of Nutritional Sciences and Toxicology, The University of California, Berkeley, California 94720 3104, USA
    J Biol Chem 278:21136-45. 2003
    ..To our knowledge, DIM is the first example of a pure androgen receptor antagonist from plants...
  2. ncbi request reprint Indole-3-carbinol induces a G1 cell cycle arrest and inhibits prostate-specific antigen production in human LNCaP prostate carcinoma cells
    Joann Zhang
    Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720 3200, USA
    Cancer 98:2511-20. 2003
    ..The objective of the current study was to characterize the cell cycle effects of I3C in human prostate carcinoma cells...
  3. ncbi request reprint 3,3'-Diindolylmethane (DIM) induces a G(1) cell cycle arrest in human breast cancer cells that is accompanied by Sp1-mediated activation of p21(WAF1/CIP1) expression
    Chibo Hong
    Department of Nutritional Sciences and Toxicology, University of California, Berkeley, CA 94720, USA
    Carcinogenesis 23:1297-305. 2002
    ..Thus, our observations have uncovered an antiproliferative pathway for DIM that implicates Sp1/Sp3-induced expression of p21 as a target for cell cycle control in human breast cancer cells...
  4. ncbi request reprint Activation and potentiation of interferon-gamma signaling by 3,3'-diindolylmethane in MCF-7 breast cancer cells
    Jacques E Riby
    Department of Nutritional Sciences and Toxicology, University of California, Berkeley, 94720 3104, USA
    Mol Pharmacol 69:430-9. 2006
    ..These results reveal novel immune activating and potentiating activities of DIM in human tumor cells that may contribute to the established effectiveness of this dietary indole against various tumors types...
  5. ncbi request reprint Potent ligand-independent estrogen receptor activation by 3,3'-diindolylmethane is mediated by cross talk between the protein kinase A and mitogen-activated protein kinase signaling pathways
    Hoyee Leong
    Department of Nutritional Sciences and Toxicology, University of California, Berkeley, California 94720, USA
    Mol Endocrinol 18:291-302. 2004
    ..These results demonstrate that DIM is a mechanistically novel activator of the ER that requires PKA-dependent phosphorylation of CREB...
  6. ncbi request reprint Fate of indole-3-carbinol in cultured human breast tumor cells
    Richard E Staub
    Department of Nutritional Sciences and Toxicology, University of California, Berkeley, California 94720, USA
    Chem Res Toxicol 15:101-9. 2002
    ..The accumulation of DIM in the nucleus suggests that this product may have a role in the cellular biological activities of I3C.3..
  7. ncbi request reprint Indole-3-carbinol and 3-3'-diindolylmethane antiproliferative signaling pathways control cell-cycle gene transcription in human breast cancer cells by regulating promoter-Sp1 transcription factor interactions
    Gary L Firestone
    Department of Molecular and Cell Biology and Cancer Research Laboratory, University of California at Berkeley, Berkeley, CA 94720 3200, USA
    J Nutr 133:2448S-2455S. 2003
    ....
  8. ncbi request reprint Indole-3-carbinol mediated cell cycle arrest of LNCaP human prostate cancer cells requires the induced production of activated p53 tumor suppressor protein
    Jocelyn C Hsu
    Department of Molecular and Cell Biology and The Cancer Research Laboratory, The University of California at Berkeley, Berkeley, CA 94720, USA
    Biochem Pharmacol 72:1714-23. 2006
    ..Taken together, our results establish that the I3C induced G1 arrest of human prostate cancer cells requires the induced production of the activated phosphorylated forms of p53, which stimulate transcription of the CDK2 inhibitor p21...
  9. ncbi request reprint Bcl-2 family-mediated apoptotic effects of 3,3'-diindolylmethane (DIM) in human breast cancer cells
    Chibo Hong
    Department of Nutritional Sciences and Toxicology, University of California, Berkeley 94720 3200, USA
    Biochem Pharmacol 63:1085-97. 2002
    ..These results demonstrate that DIM can induce apoptosis in breast cancer cells independent of estrogen receptor status by a process that is mediated by the modulated expression of the Bax/Bcl-2 family of apoptotic regulatory factors...
  10. ncbi request reprint Fate of 3,3'-diindolylmethane in cultured MCF-7 human breast cancer cells
    Richard E Staub
    Department of Nutritional Sciences and Toxicology, University of California, Berkeley, California 94720, USA
    Chem Res Toxicol 19:436-42. 2006
    ..These findings show that the uptake of DIM by membranes significantly increases its localized concentration, which may contribute to its biological activities...
  11. doi request reprint Direct inhibition of elastase activity by indole-3-carbinol triggers a CD40-TRAF regulatory cascade that disrupts NF-kappaB transcriptional activity in human breast cancer cells
    Ida Aronchik
    Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720 3200, USA
    Cancer Res 70:4961-71. 2010
    ..Furthermore, we have established a new I3C-mediated antiproliferative cascade that has significant therapeutic potential for treatment of human cancers associated with high levels of elastase and its CD40 membrane substrate...
  12. ncbi request reprint Indole-3-carbinol activates the ATM signaling pathway independent of DNA damage to stabilize p53 and induce G1 arrest of human mammary epithelial cells
    Christine T Brew
    Department of Molecular and Cell Biology and The Cancer Research Laboratory, University of California at Berkeley, Berkeley, CA 94720 3200, USA
    Int J Cancer 118:857-68. 2006
    ....
  13. ncbi request reprint DIM stimulates IFNgamma gene expression in human breast cancer cells via the specific activation of JNK and p38 pathways
    Ling Xue
    Department of Nutritional Sciences and Toxicology, University of California, 119 Morgan Hall, Berkeley, CA 94720 3104, USA
    Oncogene 24:2343-53. 2005
    ..These results establish that DIM-induced IFNgamma expression in human breast tumor cells is mediated by activation of both JNK and p38 pathways, which is ultimately dependent on intracellular calcium signaling...
  14. ncbi request reprint 3,3'-Diindolylmethane inhibits angiogenesis and the growth of transplantable human breast carcinoma in athymic mice
    Xiaofei Chang
    Department of Nutritional Sciences and Toxicology, University of California, Berkeley, CA 94720, USA
    Carcinogenesis 26:771-8. 2005
    ..This is the first study to show that DIM can strongly inhibit the development of human breast tumor in a xenograft model and to provide evidence for the antiangiogenic properties of this dietary indole...
  15. ncbi request reprint Indole-3-carbinol stimulates transcription of the interferon gamma receptor 1 gene and augments interferon responsiveness in human breast cancer cells
    Urmi Chatterji
    Department of Molecular and Cell Biology and The Cancer Research Laboratory, University of California at Berkeley, Berkeley, CA 94720 3200, USA
    Carcinogenesis 25:1119-28. 2004
    ..Our results suggest that one mechanism by which I3C mediates these anticancer effects is by stimulating expression of the IFNgammaR1 and augmenting the IFNgamma response in MCF-7 human breast cancer cells...
  16. ncbi request reprint Effects of analogs of indole-3-carbinol cyclic trimerization product in human breast cancer cells
    Ling Xue
    Department of Nutritional Sciences and Toxicology, 119 Morgan Hall, University of California, Berkeley, CA 94720, USA
    Chem Biol Interact 152:119-29. 2005
    ....
  17. pmc 3,3'-Diindolylmethane induces a G(1) arrest in human prostate cancer cells irrespective of androgen receptor and p53 status
    Omar I Vivar
    Department of Nutritional Sciences and Toxicology, 119 Morgan Hall, University of California, Berkeley, CA 94720, USA
    Biochem Pharmacol 78:469-76. 2009
    ..The Sp1 and p38 MAPK pathways mediate the DIM cell cycle regulatory effect in DU145 cells...
  18. pmc The dietary phytochemical indole-3-carbinol is a natural elastase enzymatic inhibitor that disrupts cyclin E protein processing
    Hanh H Nguyen
    Department of Molecular and Cell Biology and Cancer Research Laboratory, University of California, Berkeley, CA 94720, USA
    Proc Natl Acad Sci U S A 105:19750-5. 2008
    ....
  19. pmc 3,3'-diindolylmethane reduces levels of HIF-1alpha and HIF-1 activity in hypoxic cultured human cancer cells
    Jacques E Riby
    Department of Nutritional Sciences and Toxicology, 217 Morgan Hall, University of California, Berkeley, CA 94720, USA
    Biochem Pharmacol 75:1858-67. 2008
    ..These studies are the first to show that DIM can decrease the accumulation and activity of the key angiogenesis regulatory factor, HIF-1alpha, in hypoxic tumor cells...
  20. pmc N-Alkoxy derivatization of indole-3-carbinol increases the efficacy of the G1 cell cycle arrest and of I3C-specific regulation of cell cycle gene transcription and activity in human breast cancer cells
    Sarah M Jump
    Department of Molecular and Cell Biology and The Cancer Research Laboratory, University of California at Berkeley, Berkeley, CA 94720 3200, USA
    Biochem Pharmacol 75:713-24. 2008
    ....
  21. ncbi request reprint Inhibition of growth factor-induced Ras signaling in vascular endothelial cells and angiogenesis by 3,3'-diindolylmethane
    Xiaofei Chang
    Department of Nutritional Sciences and Toxicology, University of California, Berkeley, CA 94720, USA
    Carcinogenesis 27:541-50. 2006
    ..These data provide evidence that DIM inhibits Ras signaling induced by VEGF and other growth factors, which interferes with its downstream biological effects necessary for angiogenesis...
  22. ncbi request reprint 3,3'-Diindolylmethane is a novel mitochondrial H(+)-ATP synthase inhibitor that can induce p21(Cip1/Waf1) expression by induction of oxidative stress in human breast cancer cells
    Yixuan Gong
    Department of Nutritional Sciences and Toxicology, University of California, Berkeley, California 94720, USA
    Cancer Res 66:4880-7. 2006
    ..Thus, we have established the critical role of enhanced mitochondrial ROS release in 3,3'-diindolylmethane-induced p21 up-regulation in human breast cancer cells...
  23. ncbi request reprint Indole-3-carbinol (I3C) inhibits cyclin-dependent kinase-2 function in human breast cancer cells by regulating the size distribution, associated cyclin E forms, and subcellular localization of the CDK2 protein complex
    Hanh H Garcia
    Department of Molecular and Cell Biology and The Cancer Research Laboratory, The University of California at Berkeley, Berkeley, California 94720, USA
    J Biol Chem 280:8756-64. 2005
    ....
  24. pmc 3,3'-Diindolylmethane stimulates murine immune function in vitro and in vivo
    Ling Xue
    Department of Nutritional Sciences and Toxicology, University of California, Berkeley, CA 94720 3104, USA
    J Nutr Biochem 19:336-44. 2008
    ..Thus, DIM is a potent stimulator of immune function. This property might contribute to the cancer inhibitory effects of this indole...
  25. ncbi request reprint Pilot study: effect of 3,3'-diindolylmethane supplements on urinary hormone metabolites in postmenopausal women with a history of early-stage breast cancer
    Kathie M Dalessandri
    Department of Molecular and Cell Biology, University of California, Berkeley, 94720 3200, USA
    Nutr Cancer 50:161-7. 2004
    ..020), DIM (P =0. 045), and cortisol (P = 0.039), and a nonsignificant increase of 47% in the 2-OHE1/16alpha-OHE1 ratio from 1.46 to 2.14 (P=0.059). In this pilot study, DIM increased the 2-hydroxylation of estrogen urinary metabolites...
  26. pmc Selective activation of estrogen receptor-beta target genes by 3,3'-diindolylmethane
    Omar I Vivar
    Department of Nutritional Science and Toxicology, University of California, Berkeley, Berkeley, California 94720 3104, USA
    Endocrinology 151:1662-7. 2010
    ..Our results demonstrate that DIM is a new class of ERbeta-selective compounds, because it does not bind to ERbeta, but instead it selectively recruits ERbeta and coactivators to target genes...
  27. pmc Indole-3-carbinol inhibits MDA-MB-231 breast cancer cell motility and induces stress fibers and focal adhesion formation by activation of Rho kinase activity
    Christine T Brew
    Department of Molecular and Cell Biology, The University of California at Berkeley, Berkeley, CA 94720 3200, USA
    Int J Cancer 124:2294-302. 2009
    ..Taken together, our data demonstrate that I3C induces stress fibers and peripheral focal adhesions in a Rho kinase-dependent manner that leads to an inhibition of motility in human breast cancer cells...
  28. ncbi request reprint Convenient synthesis of 5,6,11,12,17,18- hexahydrocyclononal[1,2-b:4,5-b':7,8-b'']triindole, a novel phytoestrogen
    Richard E Staub
    Department of Nutritional Sciences and Toxicology, University of California, Berkeley, California 94720 3104, USA
    J Org Chem 68:167-9. 2003
    ..For the procedure, gramine is treated with dimethyl sulfate and sodium in ethanol at room temperature. Quenching of the reaction with water and workup of the product provides CTr in approximately 75% yield...
  29. ncbi request reprint 3,3'-diindolylmethane is a novel topoisomerase IIalpha catalytic inhibitor that induces S-phase retardation and mitotic delay in human hepatoma HepG2 cells
    Yixuan Gong
    Department of Nutritional Sciences and Toxicology, 119 Morgan Hall, University of California, Berkeley, CA 94720 3104, USA
    Mol Pharmacol 69:1320-7. 2006
    ..Our results identified a new mode of action for this intriguing dietary component that might be exploited for therapeutic development...
  30. ncbi request reprint Indole-3-carbinol inhibition of androgen receptor expression and downregulation of androgen responsiveness in human prostate cancer cells
    Jocelyn C Hsu
    Department of Molecular and Cell Biology, The University of California at Berkeley, CA 94720, USA
    Carcinogenesis 26:1896-904. 2005
    ..Taken together, our results demonstrate that I3C represses AR expression and responsiveness in LNCaP cells as a part of its antiproliferative mechanism...
  31. ncbi request reprint Indole-3-carbinol selectively uncouples expression and activity of estrogen receptor subtypes in human breast cancer cells
    Shyam N Sundar
    Department of Molecular and Cell Biology, 591 LSA, University of California at Berkeley, Berkeley, California 94720 3200 USA
    Mol Endocrinol 20:3070-82. 2006
    ....
  32. pmc Liquiritigenin is a plant-derived highly selective estrogen receptor beta agonist
    Jennifer E Mersereau
    Department of Obstetrics, Gynecology and Reproductive Sciences and Center for Reproductive Sciences, Cellular and Molecular Pharmacology, University of California, San Francisco, CA, United States
    Mol Cell Endocrinol 283:49-57. 2008
    ..In a mouse xenograph model, liquiritigenin did not stimulate uterine size or tumorigenesis of MCF-7 breast cancer cells. Our results demonstrate that some plants contain highly selective estrogens for ERbeta...

Research Grants10

  1. DIETARY INDOLES AS BREAST TUMOR CELL GROWTH INHIBITORS
    LEONARD BJELDANES; Fiscal Year: 1999
    ..To determine if the growth inhibitory effects of the I3C conversion products depend on AhR, AhR antisense blocking experiments will be conducted. ..