James Birchler

Summary

Affiliation: University of Missouri
Country: USA

Publications

  1. pmc Single-gene detection and karyotyping using small-target fluorescence in situ hybridization on maize somatic chromosomes
    Jonathan C Lamb
    Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211, USA
    Genetics 175:1047-58. 2007
  2. pmc Unraveling the genetic basis of hybrid vigor
    James A Birchler
    Division of Biological Sciences, University of Missouri, Columbia, 65211, USA
    Proc Natl Acad Sci U S A 103:12957-8. 2006
  3. pmc Telomere-mediated chromosomal truncation in maize
    Weichang Yu
    Division of Biological Sciences, University of Missouri, Columbia, MO 65211, USA
    Proc Natl Acad Sci U S A 103:17331-6. 2006
  4. doi request reprint Cellular reactions to gene dosage imbalance: genomic, transcriptomic and proteomic effects
    Reiner A Veitia
    INSERM U567, Team21 Genomics and Epigenetics of Placental Diseases, Genetics and Development Department, Institut Cochin, 75014 Paris, France
    Trends Genet 24:390-7. 2008
  5. pmc Reflections on the inhibition of RNAi by cell death signaling
    James A Birchler
    Division of Biological Sciences, University of Missouri, Columbia, MO USA
    Fly (Austin) 5:337-9. 2011
  6. doi request reprint Aneuploidy in plants and flies: the origin of studies of genomic imbalance
    James A Birchler
    Division of Biological Sciences, University of Missouri, Columbia, MO 65211, United States
    Semin Cell Dev Biol 24:315-9. 2013
  7. pmc Gene balance hypothesis: connecting issues of dosage sensitivity across biological disciplines
    James A Birchler
    Division of Biological Sciences, University of Missouri, Columbia, MO 65211, USA
    Proc Natl Acad Sci U S A 109:14746-53. 2012
  8. doi request reprint Insights from paleogenomic and population studies into the consequences of dosage sensitive gene expression in plants
    James A Birchler
    Division of Biological Sciences, University of Missouri, 311 Tucker Hall, Columbia, MO 65211, United States
    Curr Opin Plant Biol 15:544-8. 2012
  9. doi request reprint Messing with mendel
    James A Birchler
    Division of Biological Sciences, University of Missouri, Columbia, MO 65211, USA Electronic address
    Dev Cell 23:678-9. 2012
  10. pmc Retrotransposon insertion targeting: a mechanism for homogenization of centromere sequences on nonhomologous chromosomes
    James A Birchler
    Division of Biological Sciences, University of Missouri at Columbia, Columbia, Missouri 65211, USA
    Genes Dev 26:638-40. 2012

Research Grants

  1. DOSAGE COMPENSATION IN DROSOPHILA
    James Birchler; Fiscal Year: 2006
  2. DOSAGE COMPENSATION IN DROSOPHILA
    James A Birchler; Fiscal Year: 2010

Collaborators

Detail Information

Publications78

  1. pmc Single-gene detection and karyotyping using small-target fluorescence in situ hybridization on maize somatic chromosomes
    Jonathan C Lamb
    Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211, USA
    Genetics 175:1047-58. 2007
    ..The probes were demonstrated to produce signals in several wild relatives of maize, including Zea luxurians, Z. diploperennis, and Tripsacum dactyloides...
  2. pmc Unraveling the genetic basis of hybrid vigor
    James A Birchler
    Division of Biological Sciences, University of Missouri, Columbia, 65211, USA
    Proc Natl Acad Sci U S A 103:12957-8. 2006
  3. pmc Telomere-mediated chromosomal truncation in maize
    Weichang Yu
    Division of Biological Sciences, University of Missouri, Columbia, MO 65211, USA
    Proc Natl Acad Sci U S A 103:17331-6. 2006
    ..These results demonstrate that telomere-mediated chromosomal truncation operates in plant species. This technology will be useful for chromosomal engineering in maize as well as other plant species...
  4. doi request reprint Cellular reactions to gene dosage imbalance: genomic, transcriptomic and proteomic effects
    Reiner A Veitia
    INSERM U567, Team21 Genomics and Epigenetics of Placental Diseases, Genetics and Development Department, Institut Cochin, 75014 Paris, France
    Trends Genet 24:390-7. 2008
    ....
  5. pmc Reflections on the inhibition of RNAi by cell death signaling
    James A Birchler
    Division of Biological Sciences, University of Missouri, Columbia, MO USA
    Fly (Austin) 5:337-9. 2011
    ..Endogenous transposable element expression is increased and there is an overall reduction in their corresponding siRNAs. The implications of this finding for the conduct of RNAi and potential reasons for its existence are discussed...
  6. doi request reprint Aneuploidy in plants and flies: the origin of studies of genomic imbalance
    James A Birchler
    Division of Biological Sciences, University of Missouri, Columbia, MO 65211, United States
    Semin Cell Dev Biol 24:315-9. 2013
    ..This effect can produce dosage compensation when it occurs for a gene that is also present in the varied region. Some open questions in the field of aneuploidy research are discussed...
  7. pmc Gene balance hypothesis: connecting issues of dosage sensitivity across biological disciplines
    James A Birchler
    Division of Biological Sciences, University of Missouri, Columbia, MO 65211, USA
    Proc Natl Acad Sci U S A 109:14746-53. 2012
    ..Gene balance effects are hypothesized to result from stoichiometric differences among members of macromolecular complexes, the interactome, and signaling pathways. The implications of gene balance are discussed...
  8. doi request reprint Insights from paleogenomic and population studies into the consequences of dosage sensitive gene expression in plants
    James A Birchler
    Division of Biological Sciences, University of Missouri, 311 Tucker Hall, Columbia, MO 65211, United States
    Curr Opin Plant Biol 15:544-8. 2012
    ..These and other studies described suggest there are negative fitness consequences when an imbalance occurs for members of macromolecular complexes including regulatory functions...
  9. doi request reprint Messing with mendel
    James A Birchler
    Division of Biological Sciences, University of Missouri, Columbia, MO 65211, USA Electronic address
    Dev Cell 23:678-9. 2012
    ..In a recent report in Nature, de Vanssay and colleagues (2012) describe in Drosophila an operationally analogous phenomenon to paramutation that is mediated by piwi-interacting RNAs...
  10. pmc Retrotransposon insertion targeting: a mechanism for homogenization of centromere sequences on nonhomologous chromosomes
    James A Birchler
    Division of Biological Sciences, University of Missouri at Columbia, Columbia, Missouri 65211, USA
    Genes Dev 26:638-40. 2012
    ..In two different Arabidopsis species, a related element was recognized in which the specificity for such targeting was altered. These observations provide a partial explanation for how homogenization of centromere DNA sequences occurs...
  11. doi request reprint Synthetic chromosome platforms in plants
    Robert T Gaeta
    Division of Biological Sciences, University of Missouri, Columbia, MO 65211, USA
    Annu Rev Plant Biol 63:307-30. 2012
    ....
  12. pmc What's in a centromere?
    Jonathan C Lamb
    Division of Biological Sciences, University of Missouri, Columbia, MO 65211, USA
    Genome Biol 5:239. 2004
    ....
  13. pmc The role of DNA sequence in centromere formation
    Jonathan C Lamb
    University of Missouri, Division of Biological Sciences, Columbia, MO 65211, USA
    Genome Biol 4:214. 2003
    ..Several groups have recently succeeded in analyzing centromeric sequences in human, Drosophila and Arabidopsis, providing new insights into the importance of DNA sequence for centromere function...
  14. pmc Heterosis
    James A Birchler
    Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211, USA
    Plant Cell 22:2105-12. 2010
    ..While we do not provide a comprehensive model to explain the phenomenology of heterosis, we provide the details of what needs to be explained and a direction of pursuit that we feel should be fruitful...
  15. pmc Biological consequences of dosage dependent gene regulatory systems
    James A Birchler
    Division of Biological Sciences, Tucker Hall, University of Missouri, Columbia, MO 65211, USA
    Biochim Biophys Acta 1769:422-8. 2007
    ..In this article we will summarize the evidence for this potential synthesis...
  16. pmc The gene balance hypothesis: from classical genetics to modern genomics
    James A Birchler
    Division of Biological Sciences, University of Missouri, Columbia, MO 65211, USA
    Plant Cell 19:395-402. 2007
  17. doi request reprint Molecular biology. Slicing and dicing for small RNAs
    James A Birchler
    Division of Biological Sciences, University of Missouri, Columbia, MO 65211, USA
    Science 320:1023-4. 2008
  18. doi request reprint Plant engineered minichromosomes and artificial chromosome platforms
    J A Birchler
    Division of Biological Sciences, University of Missouri, Columbia, MO 65211, USA
    Cytogenet Genome Res 120:228-32. 2008
    ..The uses of engineered minichromosomes are discussed...
  19. doi request reprint A tale of two centromeres--diversity of structure but conservation of function in plants and animals
    James A Birchler
    Division of Biological Sciences, University of Missouri, Columbia, MO 65211, USA
    Funct Integr Genomics 9:7-13. 2009
    ....
  20. doi request reprint Maize centromeres: structure, function, epigenetics
    James A Birchler
    Division of Biological Sciences, University of Missouri Columbia, Columbia, Missouri 65211 7400, USA
    Annu Rev Genet 43:287-303. 2009
    ..Under some circumstances, inactive centromeres can exhibit reactivation at their formerly inactive sites. This observation suggests that a DNA-based topological component also operates for centromere identity...
  21. pmc The gene balance hypothesis: implications for gene regulation, quantitative traits and evolution
    James A Birchler
    Division of Biological Sciences, University of Missouri, Columbia, MO 65211, USA
    New Phytol 186:54-62. 2010
    ....
  22. pmc Ubiquitous RNA-dependent RNA polymerase and gene silencing
    James A Birchler
    Division of Biological Sciences, University of Missouri, Columbia, MO 65211, USA
    Genome Biol 10:243. 2009
    ..The discovery of a novel RNA-dependent RNA polymerase activity with a eukaryote-wide distribution raises new questions about the roles and mechanisms of gene silencing by small RNAs...
  23. doi request reprint Reflections on studies of gene expression in aneuploids
    James A Birchler
    Division of Biological Sciences, Tucker Hall, University of Missouri, Columbia, MO 65211, USA
    Biochem J 426:119-23. 2010
    ..A careful documentation of the modulations of gene expression in aneuploids should provide insight into the nature of cancerous cells and the basis of aneuploid syndromes...
  24. doi request reprint Epigenetic aspects of centromere function in plants
    James A Birchler
    Division of Biological Sciences, University of Missouri, Columbia, MO 65211, USA
    Curr Opin Plant Biol 14:217-22. 2011
    ..Here we present evidence for epigenetic and structural aspects involved with centromere activity in plants...
  25. doi request reprint Re-evaluation of the function of the male specific lethal complex in Drosophila
    James Birchler
    Division of Biological Sciences, University of Missouri, Columbia, USA
    J Genet Genomics 38:327-32. 2011
    ..These observations are not consistent with the hypothesis that the MSL complex conditions dosage compensation. A synthesis is described that can account for these observations...
  26. ncbi request reprint Commonalities in compensation
    James A Birchler
    Division of Biological Sciences, University of Missouri, Columbia, 65211, USA
    Bioessays 28:565-8. 2006
    ..1,2) The results suggest that a common mechanism might operate in these different cases...
  27. ncbi request reprint Dosage-dependent gene regulation in multicellular eukaryotes: implications for dosage compensation, aneuploid syndromes, and quantitative traits
    J A Birchler
    Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211, USA
    Dev Biol 234:275-88. 2001
    ..Moreover, because the majority of dosage-dependent regulators act negatively, this property can account for the up-regulation of genes in monosomics and hemizygous sex chromosomes to achieve dosage compensation...
  28. ncbi request reprint Dosage balance in gene regulation: biological implications
    James A Birchler
    Division of Biological Sciences, 117 Tucker Hall, University of Missouri, Columbia, MO 65211 7400, USA
    Trends Genet 21:219-26. 2005
    ..In this article, we discuss the potential contribution of regulatory balance to the control of quantitative traits, hybrid vigor, genome evolution and post-zygotic speciation mechanisms...
  29. pmc In search of the molecular basis of heterosis
    James A Birchler
    Division of Biological Sciences, University of Missouri, Columbia, MO 65211, USA
    Plant Cell 15:2236-9. 2003
  30. ncbi request reprint Dosage dependent gene regulation and the compensation of the X chromosome in Drosophila males
    James A Birchler
    University of Missouri, Columbia, 117 Tucker Hall, Columbia, MO 65211 7400, USA
    Genetica 117:179-90. 2003
    ....
  31. pmc Marcus rhoades, preferential segregation and meiotic drive
    James A Birchler
    Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211, USA
    Genetics 164:835-41. 2003
  32. pmc Discovering the seeds of diversity in plant genomes
    James A Birchler
    Department of Biological Sciences, University of Missouri, Columbia, MO 65211, USA
    Genome Biol 5:323. 2004
  33. ncbi request reprint Heterochromatin: RNA points the way
    James A Birchler
    Division of Biological Sciences, Tucker Hall, University of Missouri, Columbia, Missouri 65211, USA
    Curr Biol 14:R759-61. 2004
    ....
  34. ncbi request reprint Making noise about silence: repression of repeated genes in animals
    J A Birchler
    Division of Biological Sciences, University of Missouri, Columbia, MO 65211 7400, USA
    Curr Opin Genet Dev 10:211-6. 2000
    ..The silencing mechanisms potentially act as a protection against high expression of transposons and viruses...
  35. ncbi request reprint RNA silencing in Drosophila
    Harsh H Kavi
    Division of Biological Sciences, University of Missouri, 117 Tucker Hall, Columbia, MO 65211, USA
    FEBS Lett 579:5940-9. 2005
    ..RNA silencing processes in Drosophila are described...
  36. ncbi request reprint Heterochromatic silencing and HP1 localization in Drosophila are dependent on the RNAi machinery
    Manika Pal-Bhadra
    Division of Biological Sciences, 117 Tucker Hall, University of Missouri, Columbia, MO 65211, USA
    Science 303:669-72. 2004
    ..These mutations result in reduction of H3 Lys9 methylation and delocalization of HP1 and HP2, most dramatically in spindle-E mutants...
  37. ncbi request reprint RNAi related mechanisms affect both transcriptional and posttranscriptional transgene silencing in Drosophila
    Manika Pal-Bhadra
    Division of Biological Sciences, 117 Tucker Hall, University of Missouri, Columbia, MO 65211, USA
    Mol Cell 9:315-27. 2002
    ..Mutations in piwi, which belongs to a gene family with members required for RNAi, block PTGS and one aspect of TGS, indicating a connection between the two types of silencing...
  38. pmc Gene expression analysis of the function of the male-specific lethal complex in Drosophila
    Manika Pal Bhadra
    Division of Biological Sciences, University of Missouri, Columbia, 65211, USA
    Genetics 169:2061-74. 2005
    ....
  39. pmc Minichromosome analysis of chromosome pairing, disjunction, and sister chromatid cohesion in maize
    Fangpu Han
    Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211 7400, USA
    Plant Cell 19:3853-63. 2007
    ..These analyses suggest that meiotic centromeric cohesion is compromised in minichromosomes depending on their size and cannot be maintained by the mechanisms used by normal-sized chromosomes...
  40. pmc The dominant inhibitory chalcone synthase allele C2-Idf (inhibitor diffuse) from Zea mays (L.) acts via an endogenous RNA silencing mechanism
    Chris B Della Vedova
    Genetics Area Program, University of Missouri, Columbia, Missouri 65211, USA
    Genetics 170:1989-2002. 2005
    ..Finally, we detected small interfering RNAs (siRNAs) in plants carrying C2-Idf, but not in plants homozygous for the wild-type C2 allele. Together, our results indicate that the inhibitory effect of C2-Idf occurs through RNA silencing...
  41. ncbi request reprint Genetics and biochemistry of RNAi in Drosophila
    Harsh H Kavi
    Division of Biological Sciences, University of Missouri, Tucker Hall, Columbia, MO 65211, USA
    Curr Top Microbiol Immunol 320:37-75. 2008
    ..Later the active component was found to be a dsRNA (Fire et al. 1998). In subsequent years, it has been found to occur in diverse eukaryotes..
  42. ncbi request reprint Characterization of a maize isochromosome 8S*8S
    Weichang Yu
    Division of Biological Sciences, University of Missouri Columbia, 65211, USA
    Genome 49:700-6. 2006
    ..The results indicate the ability of the somatic karyotyping system to recognize and characterize chromosomal aberrations...
  43. ncbi request reprint Polycomb, pairing and PIWI--RNA silencing and nuclear interactions
    Harsh H Kavi
    Division of Biological Sciences, Tucker Hall, University of Missouri, Columbia, MO 65211, USA
    Trends Biochem Sci 31:485-7. 2006
    ..These Pc-associated sequences are involved with the control of the proper expression of developmental HOX genes...
  44. pmc Misregulation of sex-lethal and disruption of male-specific lethal complex localization in Drosophila species hybrids
    Manika Pal Bhadra
    Division of Biological Sciences, University of Missouri, Columbia 65211 7400, USA
    Genetics 174:1151-9. 2006
    ..Lethal hybrid rescue (Lhr), which allows hybrid males from this cross to survive, corrects the SXL and MSL defects. The reciprocal cross of D. simulans mothers by D. melanogaster males exhibits underexpression of Sxl in embryos...
  45. ncbi request reprint Localization and transcription of a retrotransposon-derived element on the maize B chromosome
    Jonathan C Lamb
    Division of Biological Sciences, University of Missouri Columbia, Columbia, MO 65211, USA
    Chromosome Res 15:383-98. 2007
    ..Using the LTR divergence of retroelements interrupting the B-specific sequences, the minimum age of the StarkB repeat array and, by inference, of the B chromosome, was estimated to be 2 million years...
  46. ncbi request reprint Genetic variation for the response to ploidy change in Zea mays L
    Nicole C Riddle
    Division of Biological Sciences, University of Missouri Columbia, 117 Tucker Hall, Columbia, MO 65211 7400, USA
    Theor Appl Genet 114:101-11. 2006
    ..The other source is genotype specific and results in a response to ploidy change that varies among inbred lines. This finding demonstrates the existence of genetic variation for the morphological response to ploidy change in Zea mays...
  47. pmc Nonadditive gene expression in diploid and triploid hybrids of maize
    Donald L Auger
    Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211, USA
    Genetics 169:389-97. 2005
    ..Instead, transcript levels in the diploid hybrids correlate negatively with the levels in diploid inbreds. Although transcript levels were clearly nonadditive, transcript levels in triploid hybrids were affected by genomic dosage...
  48. ncbi request reprint Global analysis of siRNA-mediated transcriptional gene silencing
    Harsh H Kavi
    Division of Biological Sciences, University of Missouri Columbia, Columbia, MO 65211, USA
    Bioessays 27:1209-12. 2005
    ..Also, intergenic regions are implicated as targets in Arabidopsis...
  49. pmc Reactivation of an inactive centromere reveals epigenetic and structural components for centromere specification in maize
    Fangpu Han
    Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211 7400, USA
    Plant Cell 21:1929-39. 2009
    ..These findings also indicate a structural aspect to centromere specification revealed by the gain of activity at the site of the previously inactive sequences...
  50. ncbi request reprint Sequences associated with A chromosome centromeres are present throughout the maize B chromosome
    Jonathan C Lamb
    Division of Biological Sciences, University of Missouri, 117 Tucker Hall, Columbia, MO 65211, USA
    Chromosoma 113:337-49. 2005
    ....
  51. doi request reprint Gene expression analysis at the intersection of ploidy and hybridity in maize
    Nicole C Riddle
    Division of Biological Sciences, University of Missouri, 117 Tucker Hall, Columbia, MO 65211, USA
    Theor Appl Genet 120:341-53. 2010
    ..Overall, we find that gene expression trends mirror observations from the phenotypic studies; however, obvious mechanistic connections remain unknown...
  52. doi request reprint Agrobacterium-mediated transformation of maize (Zea mays) with Cre-lox site specific recombination cassettes in BIBAC vectors
    Juan M Vega
    Division of Biological Sciences, University of Missouri, Tucker Hall, Columbia, MO 65211, USA
    Plant Mol Biol 66:587-98. 2008
    ..The placement of the cre/lox site-specific recombination system in many locations in the maize genome will be valuable materials for gene targeting and chromosome engineering...
  53. doi request reprint Sporophytic nondisjunction of the maize B chromosome at high copy numbers
    Rick E Masonbrink
    Division of Biological Sciences, University of Missouri, Columbia, MO 65211, USA
    J Genet Genomics 37:79-84. 2010
    ..Therefore, the B's non-disjunctive capacity, previously thought to be primarily restricted to the gametophyte, is present in sporophytic cells...
  54. ncbi request reprint Comparative analysis of inbred and hybrid maize at the diploid and tetraploid levels
    Nicole C Riddle
    Division of Biological Sciences, University of Missouri, Columbia, Columbia, MO 65211 7400, USA
    Theor Appl Genet 116:563-76. 2008
    ..Overall, the results indicate that the heterotic response of tetraploid maize lines differs significantly from that of the diploid...
  55. pmc Retroelement genome painting: cytological visualization of retroelement expansions in the genera Zea and Tripsacum
    Jonathan C Lamb
    Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211 7400, USA
    Genetics 173:1007-21. 2006
    ....
  56. ncbi request reprint Organization of endoreduplicated chromosomes in the endosperm of Zea mays L
    Matthew J Bauer
    Division of Biological Sciences, University of Missouri, Columbia, MO 65211, USA
    Chromosoma 115:383-94. 2006
    ..Interploidy crosses between diploid and tetraploid derivatives of the same inbred exhibit changes in the chromatin organization of centromeres and heterochromatic knobs...
  57. ncbi request reprint Effects of reunited diverged regulatory hierarchies in allopolyploids and species hybrids
    Nicole C Riddle
    Division of Biological Sciences, University of Missouri Columbia, 117 Tucker Hall, Columbia, MO 65211, USA
    Trends Genet 19:597-600. 2003
  58. ncbi request reprint Cytological and molecular analysis of centromere misdivision in maize
    Etienne Kaszas
    Division of Biological Sciences, University of Missouri, Columbia 65211, USA
    Genome 45:759-68. 2002
    ..We conclude that healing of broken maize centromeres can be achieved through the mechanisms of addition or fusion of telomeric repeat sequences to the broken centromere...
  59. pmc Cytological visualization of DNA transposons and their transposition pattern in somatic cells of maize
    Weichang Yu
    Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211, USA
    Genetics 175:31-9. 2007
    ..This work demonstrates the utility of FISH and karyotyping in the study of transposon activity and its consequences...
  60. ncbi request reprint A hemicentric inversion in the maize line knobless Tama flint created two sites of centromeric elements and moved the kinetochore-forming region
    Jonathan C Lamb
    Division of Biological Sciences, University of Missouri Columbia, 117 Tucker Hall, Columbia, MO 65211, USA
    Chromosoma 116:237-47. 2007
    ..Although the homologous centromeres were not paired, they were always correctly oriented at anaphase and migrated to opposite poles. Additionally, recombination on 8L was severely repressed in the hybrid...
  61. ncbi request reprint A test for ectopic exchange catalyzed by Cre recombinase in maize
    Thomas S Ream
    Division of Biological Sciences, University of Missouri Columbia, Columbia, 65211, USA
    Theor Appl Genet 111:378-85. 2005
    ..Root-tip chromosome karyotype analysis was also performed on material with and without Cre expression. Chromosomal aberrations in Cre+ material were not observed above the background level...
  62. ncbi request reprint Induction of tetraploid derivatives of maize inbred lines by nitrous oxide gas treatment
    Akio Kato
    117 Tucker Hall, University of Missouri, Columbia, MO 65211, USA
    J Hered 97:39-44. 2006
    ..The successful tetraploid inbred lines were from the A188, B73, H99, Oh43, Stock 6, and W22 genetic backgrounds. Aneuploids, plants with broken chromosomes and chimeras, were also found among the treated materials...
  63. pmc High frequency of centromere inactivation resulting in stable dicentric chromosomes of maize
    Fangpu Han
    Division of Biological Sciences, University of Missouri, 117 Tucker Hall, Columbia, 65211 7400, USA
    Proc Natl Acad Sci U S A 103:3238-43. 2006
    ..The inactivation of the centromeres was stable for at least four generations. By using dicentrics from dispensable chromosomes, centromere inactivation was found to be quite common under these circumstances...
  64. ncbi request reprint Plant chromosomes from end to end: telomeres, heterochromatin and centromeres
    Jonathan C Lamb
    Division of Biological Sciences, Tucker Hall, University of Missouri, Columbia, Missouri 65211, USA
    Curr Opin Plant Biol 10:116-22. 2007
    ..Recently, it was demonstrated that the introduction of telomere sequences into plants cells causes truncation of the chromosomes, and that this technique can be used to produce artificial chromosome platforms...
  65. pmc Construction and behavior of engineered minichromosomes in maize
    Weichang Yu
    Division of Biological Sciences, 117 Tucker Hall, University of Missouri, Columbia, MO 65211, USA
    Proc Natl Acad Sci U S A 104:8924-9. 2007
    ..These platforms will provide avenues for studies on plant chromosome structure and function and for future developments in biotechnology and agriculture...
  66. pmc Centromere function and nondisjunction are independent components of the maize B chromosome accumulation mechanism
    Fangpu Han
    Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211 7400, USA
    Plant Cell 19:524-33. 2007
    ....
  67. pmc Mitochondrial DNA transfer to the nucleus generates extensive insertion site variation in maize
    Ashley N Lough
    University of Missouri, Division of Biological Sciences, Columbia 65211 7400, USA
    Genetics 178:47-55. 2008
    ..Differences were detectable, suggesting either that mtDNA is being incorporated or lost from the maize nuclear genome continuously. The results indicate that mtDNA insertions represent a major source of nuclear chromosomal variation...
  68. pmc A fluorescence in situ hybridization system for karyotyping soybean
    Seth D Findley
    Division of Plant Sciences, National Center for Soybean Biotechnology, University of Missouri, Columbia, Missouri 65211, USA
    Genetics 185:727-44. 2010
    ..The data confirm that this translocation is widespread in G. soja accessions and likely accounts for the semi-sterility found in some G. soja by G. max crosses...
  69. ncbi request reprint Advances in plant chromosome identification and cytogenetic techniques
    Akio Kato
    Division of Biological Sciences, 117 Tucker Hall, University of Missouri, Columbia, Missouri 65211 7400, USA
    Curr Opin Plant Biol 8:148-54. 2005
    ..Such karyotype analysis opens new avenues for the study of chromosomal variation and behavior, as well as for the localization of individual genes and transgenes to genomic position...
  70. doi request reprint Integrated cytogenetic map of mitotic metaphase chromosome 9 of maize: resolution, sensitivity, and banding paint development
    Tatiana V Danilova
    Division of Biological Sciences, University of Missouri Columbia, Columbia, MO 65211, USA
    Chromosoma 117:345-56. 2008
    ..The probe collection can be used as chromosomal landmarks or as a "banding paint" for the physical mapping of sequences including transgenes and BAC clones and for studying chromosomal rearrangements...
  71. ncbi request reprint Distinct chromosomal distributions of highly repetitive sequences in maize
    Jonathan C Lamb
    Division of Biological Sciences, University of Missouri Columbia, Columbia, MO 65211, USA
    Chromosome Res 15:33-49. 2007
    ..With long exposures, smaller sites of hybridization are detected at the ends of chromosomes, adjacent to the telomere tract. This distal position for knob satellites is conserved among Zea and Tripsacum species...
  72. pmc Chromosome painting using repetitive DNA sequences as probes for somatic chromosome identification in maize
    Akio Kato
    Division of Biological Sciences, Tucker Hall, University of Missouri, Columbia, MO 65211 7400
    Proc Natl Acad Sci U S A 101:13554-9. 2004
    ..The same probe mixture identifies meiotic pachytene, late prophase I, and metaphase I chromosomes. The procedure could facilitate the study of chromosomal structure and behavior and be adapted for other plant species...
  73. ncbi request reprint Engineered minichromosomes in plants
    Weichang Yu
    Division of Biological Sciences, Tucker Hall, University of Missouri Columbia, Columbia, MO 65211, USA
    Curr Opin Biotechnol 18:425-31. 2007
    ..Thus, this technique can be easily extended to all plant species. The engineered minichromosome technology can also be used in combination with site-specific recombination systems to facilitate the stacking of multiple transgenes...
  74. pmc Molecular and functional dissection of the maize B chromosome centromere
    Weiwei Jin
    Department of Horticulture, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    Plant Cell 17:1412-23. 2005
    ..Our results demonstrate that the amount of CENH3 at the B centromere can be varied, but with decreasing amounts, the function of the centromere becomes impaired...
  75. ncbi request reprint Understanding mechanisms of novel gene expression in polyploids
    Thomas C Osborn
    Dept of Agronomy, University of Wisconsin, Madison, WI 53706, USA
    Trends Genet 19:141-7. 2003
    ..New research approaches are being used to study these mechanisms and the results should provide a more complete understanding of polyploidy...
  76. ncbi request reprint RNAi-mediated pathways in the nucleus
    Marjori A Matzke
    Gregor Mendel Institute of Molecular Plant Biology, Austrian Academy of Sciences, UZA2, Pharmazie Zentrum, Althanstrasse 14 2D 541, A 1090 Vienna, Austria
    Nat Rev Genet 6:24-35. 2005
    ..Furthermore, in some organisms RNAi silences unpaired DNA regions during meiosis. These mechanisms facilitate the directed silencing of specific genomic regions...
  77. ncbi request reprint A molecular view of plant centromeres
    Jiming Jiang
    Department of Horticulture, University of Wisconsin Madison, Madison, WI 53706, USA
    Trends Plant Sci 8:570-5. 2003
    ..CR elements as well as their flanking centromeric satellite DNA are actively transcribed in maize. These data suggest that the deposition of centromeric histones might be a transcription-coupled event...
  78. pmc Centromeric retroelements and satellites interact with maize kinetochore protein CENH3
    Cathy Xiaoyan Zhong
    Department of Plant Biology, University of Georgia, Athens, Georgia 30602, USA
    Plant Cell 14:2825-36. 2002
    ..Approximately 38 and 33% of CentC and CRM are precipitated in the chromatin immunoprecipitation assay, consistent with data showing that much, but not all, of CENH3 colocalizes with CentC...

Research Grants6

  1. DOSAGE COMPENSATION IN DROSOPHILA
    James Birchler; Fiscal Year: 2006
    ..The proposed project will contribute to an understanding of how chromatin modifications become established along the length of the chromosome and how they affect gene expression. ..
  2. DOSAGE COMPENSATION IN DROSOPHILA
    James A Birchler; Fiscal Year: 2010
    ..The single active X chromosome in humans of both males and females must be up-regulated for viability. The proposed project will examine the type of mechanism responsible for this up-regulation. ..