Lalitha R Belur

Summary

Affiliation: University of Minnesota
Country: USA

Publications

  1. ncbi request reprint Gene insertion and long-term expression in lung mediated by the Sleeping Beauty transposon system
    Lalitha R Belur
    Gene Therapy Program, Institute of Human Genetics, Minneapolis, Minnesota 55455, USA
    Mol Ther 8:501-7. 2003
  2. ncbi request reprint Sleeping Beauty-mediated transposition and long-term expression in vivo: use of the LoxP/Cre recombinase system to distinguish transposition-specific expression
    Paul R Score
    Arnold and Mabel Beckman Center for Transposon Research, Gene Therapy Program, Institute of Human Genetics, Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN 55455, USA
    Mol Ther 13:617-24. 2006
  3. ncbi request reprint Lung-directed gene therapy in mice using the nonviral Sleeping Beauty transposon system
    Lalitha R Belur
    The Arnold and Mabel Beckman Center for Transposon Research, Institute of Human Genetics, Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, Minnesota 55455, USA
    Nat Protoc 2:3146-52. 2007
  4. pmc Inhibition of angiogenesis and suppression of colorectal cancer metastatic to the liver using the Sleeping Beauty Transposon System
    Lalitha R Belur
    Center for Genome Engineering, University of Minnesota, Minneapolis, MN 55455, USA
    Mol Cancer 10:14. 2011
  5. pmc Gene expression in lung and liver after intravenous infusion of polyethylenimine complexes of Sleeping Beauty transposons
    Kelly M Podetz-Pedersen
    Beckman Center for Transposon Research, Center for Genome Engineering, Institute of Human Genetics, and Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN 55455, USA
    Hum Gene Ther 21:210-20. 2010
  6. pmc Systemic correction of storage disease in MPS I NOD/SCID mice using the sleeping beauty transposon system
    Elena L Aronovich
    Department of Genetics, Cell Biology and Development, Center for Genome Engineering, University of Minnesota, Minneapolis, 55455, USA
    Mol Ther 17:1136-44. 2009
  7. doi request reprint Liver-directed gene therapy using the sleeping beauty transposon system
    Lalitha R Belur
    Arnold and Mabel Beckman Center for Transposon Research, Gene Therapy Program, Institute of Human Genetics, and Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN, USA
    Methods Mol Biol 434:267-76. 2008
  8. pmc Preferential delivery of the Sleeping Beauty transposon system to livers of mice by hydrodynamic injection
    Jason B Bell
    Department of Genetics, Cell Biology and Development, Beckman Center for Transposon Research, Institute of Human Genetics, University of Minnesota, 6 160 Jackson Hall, 321 Church Street SE, Minneapolis, Minnesota 55455, USA
    Nat Protoc 2:3153-65. 2007
  9. ncbi request reprint Counterselection and co-delivery of transposon and transposase functions for Sleeping Beauty-mediated transposition in cultured mammalian cells
    Andrea D Converse
    Beckman Center for Transposon Research, Institute of Human Genetics, Department of Genetics, Cell Biology and Development, University of Minnesota, 6 160 Jackson Hall 321 Church Street S E, Minneapolis, MN, 55455, USA
    Biosci Rep 24:577-94. 2004
  10. ncbi request reprint Direct gene transfer to the CNS prevents emergence of neurologic disease in a murine model of mucopolysaccharidosis type I
    Daniel A Wolf
    Department of Genetics, Institute of Human Genetics, University of Minnesota, Minneapolis, MN 55455, USA
    Neurobiol Dis 43:123-33. 2011

Collaborators

Detail Information

Publications16

  1. ncbi request reprint Gene insertion and long-term expression in lung mediated by the Sleeping Beauty transposon system
    Lalitha R Belur
    Gene Therapy Program, Institute of Human Genetics, Minneapolis, Minnesota 55455, USA
    Mol Ther 8:501-7. 2003
    ..We used a linker-mediated PCR technique to recover transposon flanking sequences, demonstrating transposition of pT/L into mouse chromosomal DNA of the lung...
  2. ncbi request reprint Sleeping Beauty-mediated transposition and long-term expression in vivo: use of the LoxP/Cre recombinase system to distinguish transposition-specific expression
    Paul R Score
    Arnold and Mabel Beckman Center for Transposon Research, Gene Therapy Program, Institute of Human Genetics, Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN 55455, USA
    Mol Ther 13:617-24. 2006
    ..The results also provide additional evidence for the effectiveness of the Sleeping Beauty transposon system as an in vivo DNA-mediated gene transfer strategy for achieving long-term expression...
  3. ncbi request reprint Lung-directed gene therapy in mice using the nonviral Sleeping Beauty transposon system
    Lalitha R Belur
    The Arnold and Mabel Beckman Center for Transposon Research, Institute of Human Genetics, Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, Minnesota 55455, USA
    Nat Protoc 2:3146-52. 2007
    ..The procedure can be completed within 24 h, starting from preparation of DNA-PEI complexes to analysis of transient transgene expression...
  4. pmc Inhibition of angiogenesis and suppression of colorectal cancer metastatic to the liver using the Sleeping Beauty Transposon System
    Lalitha R Belur
    Center for Genome Engineering, University of Minnesota, Minneapolis, MN 55455, USA
    Mol Cancer 10:14. 2011
    ..We investigated whether an antiangiogenic gene transfer approach using the Sleeping Beauty (SB) transposon system could be used to inhibit growth of colorectal tumors metastatic to the liver...
  5. pmc Gene expression in lung and liver after intravenous infusion of polyethylenimine complexes of Sleeping Beauty transposons
    Kelly M Podetz-Pedersen
    Beckman Center for Transposon Research, Center for Genome Engineering, Institute of Human Genetics, and Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN 55455, USA
    Hum Gene Ther 21:210-20. 2010
    ..These results provide insight into the relative effectiveness and organ specificity of these two methods of nonviral gene delivery when coupled with the Sleeping Beauty transposon system...
  6. pmc Systemic correction of storage disease in MPS I NOD/SCID mice using the sleeping beauty transposon system
    Elena L Aronovich
    Department of Genetics, Cell Biology and Development, Center for Genome Engineering, University of Minnesota, Minneapolis, 55455, USA
    Mol Ther 17:1136-44. 2009
    ....
  7. doi request reprint Liver-directed gene therapy using the sleeping beauty transposon system
    Lalitha R Belur
    Arnold and Mabel Beckman Center for Transposon Research, Gene Therapy Program, Institute of Human Genetics, and Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN, USA
    Methods Mol Biol 434:267-76. 2008
    ....
  8. pmc Preferential delivery of the Sleeping Beauty transposon system to livers of mice by hydrodynamic injection
    Jason B Bell
    Department of Genetics, Cell Biology and Development, Beckman Center for Transposon Research, Institute of Human Genetics, University of Minnesota, 6 160 Jackson Hall, 321 Church Street SE, Minneapolis, Minnesota 55455, USA
    Nat Protoc 2:3153-65. 2007
    ..Several weeks after injection, transgene expression stabilizes at approximately 1% of the level at 24 h, presumably owing to integration of the transposons into chromosomes...
  9. ncbi request reprint Counterselection and co-delivery of transposon and transposase functions for Sleeping Beauty-mediated transposition in cultured mammalian cells
    Andrea D Converse
    Beckman Center for Transposon Research, Institute of Human Genetics, Department of Genetics, Cell Biology and Development, University of Minnesota, 6 160 Jackson Hall 321 Church Street S E, Minneapolis, MN, 55455, USA
    Biosci Rep 24:577-94. 2004
    ....
  10. ncbi request reprint Direct gene transfer to the CNS prevents emergence of neurologic disease in a murine model of mucopolysaccharidosis type I
    Daniel A Wolf
    Department of Genetics, Institute of Human Genetics, University of Minnesota, Minneapolis, MN 55455, USA
    Neurobiol Dis 43:123-33. 2011
    ..The results suggest the potential of improved outcome for MPSs and other neurological diseases when a high level of gene expression can be achieved by direct, early administration of vector to the CNS...
  11. pmc Differential adeno-associated virus mediated gene transfer to sensory neurons following intrathecal delivery by direct lumbar puncture
    Lucy Vulchanova
    Department of Veterinary and Biomedical Sciences, University of Minnesota, Saint Paul, MN 55108, USA
    Mol Pain 6:31. 2010
    ..In this study we have compared expression patterns of AAV serotype 5 (AAV5)- and AAV serotype 8 (AAV8)-mediated gene transfer to sensory neurons following intrathecal delivery by direct lumbar puncture...
  12. pmc Prolonged expression of a lysosomal enzyme in mouse liver after Sleeping Beauty transposon-mediated gene delivery: implications for non-viral gene therapy of mucopolysaccharidoses
    Elena L Aronovich
    Department of Genetics, Cell Biology and Development and The Arnold and Mabel Beckman Center for Transposon Research, University of Minnesota, Minneapolis, MN 55455, USA
    J Gene Med 9:403-15. 2007
    ..The Sleeping Beauty (SB) transposon system is a non-viral vector system that can integrate precise sequences into chromosomes. We evaluated the SB transposon system as a tool for gene therapy of mucopolysaccharidosis (MPS) types I and VII...
  13. ncbi request reprint Efficient and stable transgene expression in human embryonic stem cells using transposon-mediated gene transfer
    Andrew Wilber
    The Arnold and Mabel Beckman Center for Transposon Research, Gene Therapy Program, Institute of Human Genetics, Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, Minnesota 55455, USA
    Stem Cells 25:2919-27. 2007
    ..We conclude that the SB transposon system provides an effective approach with several advantages for genetic manipulation and durable gene expression in human ES cells...
  14. ncbi request reprint Delivery of transposon DNA to lungs of mice using polyethyleneimine-DNA complexes
    Lalitha R Belur
    Institute of Human Genetics, University of Minnesota, Minneapolis, MN, USA
    Methods Mol Biol 245:137-44. 2004
  15. doi request reprint Adeno-associated virus type 2 vectors: transduction and long-term expression in cerebellar Purkinje cells in vivo is mediated by the fibroblast growth factor receptor 1 : bFGFR-1 mediates AAV2 transduction of Purkinje cells
    Lalitha R Belur
    Gene Therapy Program, Institute of Human Genetics, University of Minnesota, Minneapolis, MN 55455, USA
    Arch Virol 153:2107-10. 2008
    ..These results provide new information on fibroblast growth factor receptor-1 involvement in AAV2-mediated transduction of Purkinje cells and have important implications for AAV-mediated treatment of various cerebellar disorders...
  16. ncbi request reprint Methotrexate preconditioning allows sufficient engraftment to confer drug resistance in mice transplanted with marrow expressing drug-resistant dihydrofolate reductase activity
    Lalitha R Belur
    Dept of Genetics, Cell Biology and Development, 6 160 Jackson Hall, 321 Church St SE, University of Minnesota, Minneapolis, MN 55455, USA
    J Pharmacol Exp Ther 314:668-74. 2005
    ..We conclude that MTX can be used as a preconditioning agent for subsequent engraftment of hematopoietic stem cells, in this case conferring resistance to MTX...