David J Beebe

Summary

Affiliation: University of Wisconsin
Country: USA

Publications

  1. ncbi request reprint Physics and applications of microfluidics in biology
    David J Beebe
    Department of Biomedical Engineering, University of Wisconsin, Madison 53706, USA
    Annu Rev Biomed Eng 4:261-86. 2002
  2. pmc A microfluidic coculture and multiphoton FAD analysis assay provides insight into the influence of the bone microenvironment on prostate cancer cells
    Lauren L Bischel
    Department of Biomedical Engineering, Wisconsin Institutes for Medical Research, University of Wisconsin Madison, 1111 Highland Ave, Madison, WI, USA
    Integr Biol (Camb) 6:627-35. 2014
  3. pmc Paired diagnostic and pharmacodynamic analysis of rare non-small cell lung cancer cells enabled by the VerIFAST platform
    Benjamin P Casavant
    Department of Biomedical Engineering, Wisconsin Institutes for Medical Research, University of Wisconsin Madison, 1111 Highland Ave, Madison, WI, USA
    Lab Chip 14:99-105. 2013
  4. pmc Suspended microfluidics
    Benjamin P Casavant
    Carbone Cancer Center, Madison, WI 53705, USA
    Proc Natl Acad Sci U S A 110:10111-6. 2013
  5. pmc Enabling screening in 3D microenvironments: probing matrix and stromal effects on the morphology and proliferation of T47D breast carcinoma cells
    Sara I Montanez-Sauri
    Materials Science Program, University of Wisconsin Madison, Madison, WI, USA
    Integr Biol (Camb) 5:631-40. 2013
  6. pmc Understanding the impact of 2D and 3D fibroblast cultures on in vitro breast cancer models
    Kyung Eun Sung
    Department of Biomedical Engineering, University of Wisconsin, Madison, Wisconsin, United States of America Paul P Carbone Comprehensive Cancer Center, University of Wisconsin, Madison, Wisconsin, United States of America Laboratory of Optical and Computational Instrumentation, University of Wisconsin, Madison, Wisconsin, United States of America
    PLoS ONE 8:e76373. 2013
  7. pmc Transition to invasion in breast cancer: a microfluidic in vitro model enables examination of spatial and temporal effects
    Kyung Eun Sung
    Department of Biomedical Engineering, University of Wisconsin, Madison, WI, USA
    Integr Biol (Camb) 3:439-50. 2011
  8. doi request reprint The VerIFAST: an integrated method for cell isolation and extracellular/intracellular staining
    Benjamin P Casavant
    Department of Biomedical Engineering, Wisconsin Institutes for Medical Research, University of Wisconsin Madison, Madison, WI, USA
    Lab Chip 13:391-6. 2013
  9. pmc High-content adhesion assay to address limited cell samples
    Jay W Warrick
    Biomedical Engineering, University of Wisconsin, Madison, WI, USA
    Integr Biol (Camb) 5:720-7. 2013
  10. pmc Automation of three-dimensional cell culture in arrayed microfluidic devices
    Sara I Montanez-Sauri
    Materials Science Program, University of Wisconsin Madison, Madison, WI 53705, USA
    J Lab Autom 16:171-85. 2011

Collaborators

Detail Information

Publications105 found, 100 shown here

  1. ncbi request reprint Physics and applications of microfluidics in biology
    David J Beebe
    Department of Biomedical Engineering, University of Wisconsin, Madison 53706, USA
    Annu Rev Biomed Eng 4:261-86. 2002
    ..The focus of this review is microscale phenomena and the use of the physics of the scale to create devices and systems that provide functionality useful to the life sciences...
  2. pmc A microfluidic coculture and multiphoton FAD analysis assay provides insight into the influence of the bone microenvironment on prostate cancer cells
    Lauren L Bischel
    Department of Biomedical Engineering, Wisconsin Institutes for Medical Research, University of Wisconsin Madison, 1111 Highland Ave, Madison, WI, USA
    Integr Biol (Camb) 6:627-35. 2014
    ..The studies performed using this combined platform will lead to new insights into the mechanisms that drive prostate cancer metastasis. ..
  3. pmc Paired diagnostic and pharmacodynamic analysis of rare non-small cell lung cancer cells enabled by the VerIFAST platform
    Benjamin P Casavant
    Department of Biomedical Engineering, Wisconsin Institutes for Medical Research, University of Wisconsin Madison, 1111 Highland Ave, Madison, WI, USA
    Lab Chip 14:99-105. 2013
    ....
  4. pmc Suspended microfluidics
    Benjamin P Casavant
    Carbone Cancer Center, Madison, WI 53705, USA
    Proc Natl Acad Sci U S A 110:10111-6. 2013
    ..Suspended microfluidics brings the high degree of fluidic control and unique functionality of closed microfluidics into the highly accessible and robust platform of open microfluidics...
  5. pmc Enabling screening in 3D microenvironments: probing matrix and stromal effects on the morphology and proliferation of T47D breast carcinoma cells
    Sara I Montanez-Sauri
    Materials Science Program, University of Wisconsin Madison, Madison, WI, USA
    Integr Biol (Camb) 5:631-40. 2013
    ..The platform presented in this work enables screening for the effects of matrix and stromal compositions and show promises for providing new insights in the identification of key ECM components involved in breast cancer...
  6. pmc Understanding the impact of 2D and 3D fibroblast cultures on in vitro breast cancer models
    Kyung Eun Sung
    Department of Biomedical Engineering, University of Wisconsin, Madison, Wisconsin, United States of America Paul P Carbone Comprehensive Cancer Center, University of Wisconsin, Madison, Wisconsin, United States of America Laboratory of Optical and Computational Instrumentation, University of Wisconsin, Madison, Wisconsin, United States of America
    PLoS ONE 8:e76373. 2013
    ..This study highlights the importance of the 3D stromal microenvironment in the development of multiple cell type in vitro cancer models. ..
  7. pmc Transition to invasion in breast cancer: a microfluidic in vitro model enables examination of spatial and temporal effects
    Kyung Eun Sung
    Department of Biomedical Engineering, University of Wisconsin, Madison, WI, USA
    Integr Biol (Camb) 3:439-50. 2011
    ..Importantly, the model can be easily adapted and generalized to a variety of cell-cell signaling studies...
  8. doi request reprint The VerIFAST: an integrated method for cell isolation and extracellular/intracellular staining
    Benjamin P Casavant
    Department of Biomedical Engineering, Wisconsin Institutes for Medical Research, University of Wisconsin Madison, Madison, WI, USA
    Lab Chip 13:391-6. 2013
    ..Further, cellular staining can be performed in the device and is demonstrated for extracellular epithelial cell adhesion molecule (EpCAM), intracellular pan-cytokeratins, and Ki-67...
  9. pmc High-content adhesion assay to address limited cell samples
    Jay W Warrick
    Biomedical Engineering, University of Wisconsin, Madison, WI, USA
    Integr Biol (Camb) 5:720-7. 2013
    ..We anticipate this method will enable a new class of biological studies with potential impact in basic and translational research...
  10. pmc Automation of three-dimensional cell culture in arrayed microfluidic devices
    Sara I Montanez-Sauri
    Materials Science Program, University of Wisconsin Madison, Madison, WI 53705, USA
    J Lab Autom 16:171-85. 2011
    ..The platform allows more efficient 3D culture experiments and lays the foundation for high throughput studies of cell-ECM interactions...
  11. pmc Rapid prototyping of arrayed microfluidic systems in polystyrene for cell-based assays
    Edmond W K Young
    Department of Biomedical Engineering, Wisconsin Institutes for Medical Research, University of Wisconsin Madison, Madison, Wisconsin 53705, USA
    Anal Chem 83:1408-17. 2011
    ..The choice of materials and methods was validated for biological function in two different cell-based applications to demonstrate the versatility of our streamlined fabrication process...
  12. doi request reprint Synaptotagmin II peptide-bead conjugate for botulinum toxin enrichment and detection in microchannels
    Megan L Frisk
    Department of Biomedical Engineering, University of Wisconsin, Madison, WI 53706, USA
    Biosens Bioelectron 26:1929-35. 2011
    ..e. dilution, centrifugation, filtering), and with a "hands-on" time of only 1 h to detect 16.6 pg of BoNT/B in whole milk...
  13. pmc Kit-On-A-Lid-Assays for accessible self-contained cell assays
    Erwin Berthier
    Department of Medical Microbiology and Immunology, University of Wisconsin Madison, Madison, WI, USA
    Lab Chip 13:424-31. 2013
    ..We demonstrate that the KOALA cell-based assays can be simply operated from start to finish without any external laboratory equipment...
  14. pmc A negative selection methodology using a microfluidic platform for the isolation and enumeration of circulating tumor cells
    Benjamin P Casavant
    Department of Biomedical Engineering, University of Wisconsin Madison, Madison, WI, USA
    Methods 64:137-43. 2013
    ..Because the MCC method does not use positive selection, the method can be applied across a variety of tumor types with no changes to the process. ..
  15. doi request reprint A microscale neuron and Schwann cell coculture model for increasing detection sensitivity of botulinum neurotoxin type A
    Won S Hong
    Department of Biomedical Engineering, University of Wisconsin, Madison, WI, USA
    Toxicol Sci 134:64-72. 2013
    ..Thus, our study demonstrates two distinct strategies for increasing the sensitivity of a cell line to BoNT using coculture and microscale culture, thereby advancing assay technology for BoNT detection. ..
  16. pmc Tubeless microfluidic angiogenesis assay with three-dimensional endothelial-lined microvessels
    Lauren L Bischel
    Department of Biomedical Engineering, University of Wisconsin Madison, Madison, WI 53706, USA
    Biomaterials 34:1471-7. 2013
    ..We demonstrate the implementation and application of this technique to the study of angiogenesis in a physiologically relevant in vitro setting...
  17. pmc Assessment of enhanced autofluorescence and impact on cell microscopy for microfabricated thermoplastic devices
    Edmond W K Young
    Department of Biomedical Engineering, Wisconsin Institutes for Medical Research, University of Wisconsin Madison, 53705, United States
    Anal Chem 85:44-9. 2013
    ....
  18. doi request reprint Streamlining immunoassays with immiscible filtrations assisted by surface tension
    Scott M Berry
    Department of Biomedical Engineering, University of Wisconsin Madison, Madison, Wisconsin 53705, United States
    Anal Chem 84:5518-23. 2012
    ..Assay performance was assessed by measuring known concentrations of recombinant PSA protein. The assay was then used to measure PSA concentrations in conditioned media and human plasma samples...
  19. pmc Induced hydrophobic recovery of oxygen plasma-treated surfaces
    David J Guckenberger
    Department of Biomedical Engineering, Wisconsin Institutes for Medical Research, University of Wisconsin Madison, Madison, WI, USA
    Lab Chip 12:2317-21. 2012
    ..This method will be useful in academic and industrial settings where plasma treatment is frequently used...
  20. doi request reprint Zebrafish Entrapment By Restriction Array (ZEBRA) device: a low-cost, agarose-free zebrafish mounting technique for automated imaging
    Lauren L Bischel
    Department of Biomedical Engineering, Wisconsin Institute for Medical Research, University of Wisconsin Madison, Madison, WI, USA
    Lab Chip 13:1732-6. 2013
    ..The findings report the development of ZEBRA a device that can be used to screen for small molecules that affect leukocyte motility and inflammation using live zebrafish...
  21. ncbi request reprint Bead-based microfluidic toxin sensor integrating evaporative signal amplification
    Megan L Frisk
    Department of Biomedical Engineering, University of Wisconsin Madison, Madison, WI, USA
    Lab Chip 8:1793-800. 2008
    ..Our bead-based microfluidic platform can sense BoNT/A down to 10 pg of toxin per mL buffer solution in 3.5 h and can be adapted to sensing other toxins that operate via enzymatic cleavage of a known substrate...
  22. doi request reprint Facile and rapid DNA extraction and purification from food matrices using IFAST (immiscible filtration assisted by surface tension)
    Lindsay N Strotman
    Department of Biomedical Engineering, University of Wisconsin Madison, 1111 Highland Ave, Madison, WI 53705, USA
    Analyst 137:4023-8. 2012
    ..5 μL of sample and was accomplished in five-fold less time. The simplicity, rapidity and portability of IFAST offer significant advantages when compared to existing DNA sample preparation methods...
  23. pmc 3D microchannel co-culture: method and biological validation
    Maret Bauer
    Department of Pathology and Laboratory Medicine, University of Wisconsin Madison, Madison, WI 53792, USA
    Integr Biol (Camb) 2:371-8. 2010
    ..g. automated liquid handlers, scanners)...
  24. pmc Functional screen of paracrine signals in breast carcinoma fibroblasts
    Gui Su
    Department of Pathology and Laboratory Medicine, University of Wisconsin Madison, Madison, Wisconsin, United States of America
    PLoS ONE 7:e46685. 2012
    ..In summary, we identify a paracrine signaling network with inter-individual commonalities and differences. These findings have significant implications for the design of stroma-targeted therapies...
  25. pmc Low-volume toolbox for the discovery of immunosuppressive fungal secondary metabolites
    Erwin Berthier
    Department of Medical Microbiology and Immunology, University of Wisconsin Madison, Madison, Wisconsin, United States of America
    PLoS Pathog 9:e1003289. 2013
    ..The tools developed here will open new "function-omic" avenues downstream of the metabolomics, identification, and purification phases...
  26. pmc Microfluidic kit-on-a-lid: a versatile platform for neutrophil chemotaxis assays
    Eric K Sackmann
    Materials Science Program, Wisconsin Institute for Medical Research, Madison, WI 53705, USA
    Blood 120:e45-53. 2012
    ..These methods allow for new avenues of research while reducing the complexity, time, and sample volume requirements to perform neutrophil chemotaxis assays...
  27. pmc The actin regulatory protein HS1 interacts with Arp2/3 and mediates efficient neutrophil chemotaxis
    Peter J Cavnar
    Departments of Pediatrics and Medical Microbiology and Immunology, 4205 Microbial Sciences Building, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    J Biol Chem 287:25466-77. 2012
    ..Moreover, HS1 phosphorylation on Y222, Y378, and Y397 regulates its interaction with Arp2/3. Collectively, our findings identify a novel role for HS1 and its phosphorylation during neutrophil directed migration...
  28. pmc Weak protein-protein interactions revealed by immiscible filtration assisted by surface tension
    Scott M Berry
    Department of Biomedical Engineering, University of Wisconsin at Madison, Madison, WI 53705, USA Electronic address
    Anal Biochem 447:133-40. 2014
    ..We anticipate that this simple, rapid method to isolate intact, transient complexes will enable the discoveries of new signaling pathways, biomarkers, and drug targets. ..
  29. pmc Automated operation of immiscible filtration assisted by surface tension (IFAST) arrays for streamlined analyte isolation
    Scott M Berry
    Department of Biomedical Engineering, University of Wisconsin Madison, Madison, WI, USA
    J Lab Autom 18:206-11. 2013
    ..Automated operation of the IFAST devices resulted in more repeatable results relative to manual operation...
  30. pmc Hormonally responsive breast cancer cells in a microfluidic co-culture model as a sensor of microenvironmental activity
    Jessica D Lang
    University of Wisconsin Madison Carbone Comprehensive Cancer Center, Madison, WI 53705, USA
    Integr Biol (Camb) 5:807-16. 2013
    ..These data suggest an expanded utility for the tumor biomarker ERα, in which the combination of dynamic regulation of ERα protein and growth in a breast cancer biosensor cell become a read-out of the microenvironmental activity...
  31. pmc Hedgehog signaling in myofibroblasts directly promotes prostate tumor cell growth
    Maribella Domenech
    Department of Biomedical Engineering and Wisconsin Institute for Medical Research, University of Wisconsin Madison, Madison, Wisconsin 53705, USA
    Integr Biol (Camb) 4:142-52. 2012
    ..By isolating the tropic effect of Hh pathway activation in prostate stroma, we have taken the first step toward identifying cell-specific mechanisms that mediate the effect of paracrine Hh signaling on tumor growth...
  32. pmc Characterizing asthma from a drop of blood using neutrophil chemotaxis
    Eric Karl Heinz Sackmann
    Materials Science Program, Department of Biomedical Engineering, Wisconsin Institute for Medical Research, Division of Allergy, Pulmonary and Critical Care Medicine, Department of Medicine, and Departments of Biostatistics and Medical Informatics and Pediatrics, University of Wisconsin Madison, Madison, WI 53705
    Proc Natl Acad Sci U S A 111:5813-8. 2014
    ..This study identifies neutrophil chemotaxis velocity as a potential biomarker for asthma, and we demonstrate a microfluidic technology that was used in a clinical setting to perform these measurements. ..
  33. pmc Quantification of kinase activity in cell lysates via photopatterned macroporous poly(ethylene glycol) hydrogel arrays in microfluidic channels
    Andrew G Lee
    Department of Chemical and Biological Engineering, University of Wisconsin Madison, 1415 Engineering Drive, Madison, WI 53706, USA
    Biomed Microdevices 14:247-57. 2012
    ....
  34. pmc Microfluidic cell culture and its application in high-throughput drug screening: cardiotoxicity assay for hERG channels
    Xiaojing Su
    Department of Biomedical Engineering and Wisconsin Institutes for Medical Research, University of Wisconsin Madison, Madison, WI, USA
    J Biomol Screen 16:101-11. 2011
    ..Finally, treatment of WT-hERG cells with 5 different drugs suggests that PS (and COP) microchannels were more suitable than PDMS microchannels for drug screening applications, particularly for tests involving hydrophobic drug molecules...
  35. pmc Hax1 regulates neutrophil adhesion and motility through RhoA
    Peter J Cavnar
    Department of Pediatrics, University of Wisconsin Madison, Madison, WI 53706, USA
    J Cell Biol 193:465-73. 2011
    ..Accordingly, activation of RhoA is sufficient to rescue adhesion of Hax1-deficient neutrophils. Together, our findings identify Hax1 as a novel regulator of neutrophil uropod detachment and chemotaxis through RhoA...
  36. pmc Nucleic acid sample preparation using spontaneous biphasic plug flow
    Peter C Thomas
    Department of Biomedical Engineering and Wisconsin Institute for Medical Research, University of Wisconsin Madison, Madison, Wisconsin 53705, United States
    Anal Chem 85:8641-6. 2013
    ..The results demonstrate the utility of the current technique for nucleic acid purification for clinical purposes, and the overall approach provides a potential method to implement nucleic acid testing in low-resource settings. ..
  37. pmc Microscale functional cytomics for studying hematologic cancers
    Edmond W K Young
    Department of Biomedical Engineering, University of Wisconsin Madison, 53705, USA
    Blood 119:e76-85. 2012
    ..Our platform can be applied to myriad biological questions, enabling high-content functional cytomics of primary hematologic malignancies...
  38. doi request reprint The present and future role of microfluidics in biomedical research
    Eric K Sackmann
    Materials Science Program, Department of Biomedical Engineering, Wisconsin Institutes for Medical Research, University of Wisconsin Madison, 1111 Highland Avenue, Madison, Wisconsin 53705 2275, USA
    Nature 507:181-9. 2014
    ..We also suggest directions that biologists, engineers and clinicians can take to help this technology live up to its potential. ..
  39. pmc Characterization of molecules binding to the 70K N-terminal region of fibronectin by IFAST purification coupled with mass spectrometry
    S Farshid Moussavi-Harami
    Medical Scientist Training Program, University of Wisconsin, Madison, Wisconsin 53705, USA
    J Proteome Res 12:3393-404. 2013
    ....
  40. pmc Effect of microculture on cell metabolism and biochemistry: do cells get stressed in microchannels?
    Xiaojing Su
    Department of Biomedical Engineering, University of Wisconsin Madison, Wisconsin Institutes for Medical Research, 1111 Highland Avenue, Madison, Wisconsin 53705, United States
    Anal Chem 85:1562-70. 2013
    ..This work illuminates how biochemical properties of cells differ in macro- and microculture and suggests strategies that can be used to modify cell culture protocols for future studies involving miniaturized culture platforms...
  41. pmc A practical method for patterning lumens through ECM hydrogels via viscous finger patterning
    Lauren L Bischel
    Department of Biomedical Engineering, University of Wisconsin Madison, Madison, WI 53705 2275, USA
    J Lab Autom 17:96-103. 2012
    ....
  42. doi request reprint Circulating tumor cells: getting more from less
    Joshua M Lang
    Carbone Cancer Center, University of Wisconsin, Madison, WI 53705, USA
    Sci Transl Med 4:141ps13. 2012
    ....
  43. pmc Streamlining gene expression analysis: integration of co-culture and mRNA purification
    Scott M Berry
    Departments of Biomedical Engineering, University of Wisconsin Madison, 1111 Highland Ave, Madison, WI 53705, USA
    Integr Biol (Camb) 6:224-31. 2014
    ..We anticipate that this platform will be used for streamlined analysis of paracrine signaling events as well as for screening potential drugs and/or patient samples. ..
  44. doi request reprint Cell culture models in microfluidic systems
    Ivar Meyvantsson
    BellBrook Labs, LLC, Madison, Wisconsin 53711, USA
    Annu Rev Anal Chem (Palo Alto Calif) 1:423-49. 2008
    ..Finally, we consider the integration of analytical elements, ways to achieve high throughput, and the design constraints imposed by cell biology applications...
  45. pmc Both LRP5 and LRP6 receptors are required to respond to physiological Wnt ligands in mammary epithelial cells and fibroblasts
    Shruti Goel
    McArdle Laboratory for Cancer Research, University of Wisconsin, Madison, Wisconsin 53706, USA
    J Biol Chem 287:16454-66. 2012
    ..These data have implications for stem cell biology and for the analysis of the oncogenicity of LRP receptors that are often overexpressed in breast tumors...
  46. pmc Pipette-friendly laminar flow patterning for cell-based assays
    Erwin Berthier
    University of Wisconsin Madison, Biomedical Engineering, 1111 Highland Av, Madison, WI 53705, USA
    Lab Chip 11:2060-5. 2011
    ....
  47. pmc Purification of cell subpopulations via immiscible filtration assisted by surface tension (IFAST)
    Scott M Berry
    Department of Biomedical Engineering, University of Wisconsin Madison, 1111 Highland Ave, Madison, WI 53705, USA
    Biomed Microdevices 13:1033-42. 2011
    ..Finally, cells were observed to remain viable and proliferative following traverse through the immiscible phase, indicating that this process is suitable for a variety of downstream assays, including those requiring intact living cells...
  48. pmc From the cellular perspective: exploring differences in the cellular baseline in macroscale and microfluidic cultures
    Amy L Paguirigan
    Department of Biomedical Engineering, University of Wisconsin Madison, WI, USA
    Integr Biol (Camb) 1:182-95. 2009
    ..Additional areas of study that will further characterize and validate microscale culture are discussed...
  49. pmc Selective nucleic acid removal via exclusion (SNARE): capturing mRNA and DNA from a single sample
    Lindsay Strotman
    Department of Biomedical Engineering, Wisconsin Institutes for Medical Research, University of Wisconsin Madison, 1111 Highland Avenue, Madison, Wisconsin53706, United States
    Anal Chem 85:9764-70. 2013
    ....
  50. ncbi request reprint Do-it-yourself microelectrophoresis chips with integrated sample recovery
    Swomitra K Mohanty
    Department of Biomedical Engineering, University of Wisconsin Madison, Madison, WI, USA
    Electrophoresis 27:3772-8. 2006
    ..The microPAGE device is simple to fabricate, benefits from microscale analysis, and includes an on-chip collection scheme that interfaces the macroworld with the microworld...
  51. pmc Fluorescence-Based Assessment of Plasma-Induced Hydrophilicity in Microfluidic Devices via Nile Red Adsorption and Depletion
    David J Guckenberger
    Department of Biomedical Engineering, Wisconsin Institutes for Medical Research, University of Wisconsin Madison, 1111 Highland Avenue, Madison, Wisconsin 53705, United States
    Anal Chem 86:7258-63. 2014
    ..We showed that these variations have a significant impact on cell culture, and thus the design of cell-based microfluidic assays must consider this phenomenon to obtain repeatable and homogeneous results. ..
  52. pmc Expanding the available assays: adapting and validating In-Cell Westerns in microfluidic devices for cell-based assays
    Amy L Paguirigan
    Department of Biomedical Engineering, Wisconsin Institutes for Medical Research, University of Wisconsin Madison, Madison, Wisconsin 53726, USA
    Assay Drug Dev Technol 8:591-601. 2010
    ..The use of passive pumping for sample delivery and laser scanning for analysis opens the door to high-throughput quantitative microfluidic cell-based assays that integrate seamlessly with existing high-throughput infrastructure...
  53. pmc Efficient sample preparation from complex biological samples using a sliding lid for immobilized droplet extractions
    Benjamin P Casavant
    Department of Biomedical Engineering, Wisconsin Institutes for Medical Research, University of Wisconsin, Madison, Wisconsin 53705, United States
    Anal Chem 86:6355-62. 2014
    ..SLIDE demonstrates a simple easy to use platform for sample preparation on a range of complex biological samples. ..
  54. ncbi request reprint A Cell Programmable Assay (CPA) chip
    Jongil Ju
    Department of Biomedical Engineering and Wisconsin Institutes for Medical Research, University of Wisconsin Madison, Madison, WI 53705, USA
    Lab Chip 10:2071-6. 2010
    ..The sCPA and dCPA chips can be readily modified to apply to a wide variety of common cell culture methods and procedures...
  55. pmc Development of macroporous poly(ethylene glycol) hydrogel arrays within microfluidic channels
    Andrew G Lee
    Department of Chemical and Biological Engineering, University of Wisconsin Madison, 1415 Engineering Drive, Madison, Wisconsin 53706, United States
    Biomacromolecules 11:3316-24. 2010
    ..These results suggest that hydrogel macroporosity can be tuned to regulate macromolecular transport in applications such as tissue engineering and protein arrays...
  56. ncbi request reprint HIV viral RNA extraction in wax immiscible filtration assisted by surface tension (IFAST) devices
    Scott M Berry
    Department of Biomedical Engineering, University of Wisconsin Madison, Madison, Wisconsin Electronic address
    J Mol Diagn 16:297-304. 2014
    ..Using this technology, we have demonstrated accurate and repeatable measurements of viral load on samples with as low as 50 copies per milliliter of sample...
  57. pmc Screening the cellular microenvironment: a role for microfluidics
    Jay W Warrick
    Department of Biomedical Engineering, University of Wisconsin, Madison, WI 53706 1609, USA
    IEEE Rev Biomed Eng 1:75-93. 2008
    ..Microfluidic technology shows promise for simultaneous control of multiple parameters of the microenvironment and can provide a base for scaling advanced cell-based experiments into automated high-throughput formats...
  58. doi request reprint An inertia enhanced passive pumping mechanism for fluid flow in microfluidic devices
    Pedro J Resto
    Materials Science Program, University of Wisconsin, Madison, WI 53706, USA
    Lab Chip 12:2221-8. 2012
    ..Momentum is leveraged to create rapid fluid exchanges, instantaneous flow reversal, filling and mixing inside the microfluidic device...
  59. ncbi request reprint Hydrogel-based reconfigurable components for microfluidic devices
    Dongshin Kim
    Department of Mechanical Engineering, University of Wisconsin, Madison, WI 53706, USA
    Lab Chip 7:193-8. 2007
    ....
  60. pmc Cellular observations enabled by microculture: paracrine signaling and population demographics
    Maribella Domenech
    University of Wisconsin Madison, 3144 Engineering Centers Building, 1550 Engineering Drive, Madison, WI 53706, USA
    Integr Biol (Camb) 1:267-74. 2009
    ..In this example the data support the hypothesis that a growth promoting subpopulation of cells exists in the mouse mammary gland...
  61. pmc Infection on a chip: a microscale platform for simple and sensitive cell-based virus assays
    Ying Zhu
    Department of Chemical and Biological Engineering, University of Wisconsin Madison, Madison, WI 53706, USA
    Biomed Microdevices 11:565-70. 2009
    ....
  62. pmc Fundamentals of microfluidic cell culture in controlled microenvironments
    Edmond W K Young
    Department of Biomedical Engineering, Wisconsin Institutes for Medical Research, University of Wisconsin Madison, 1111 Highland Avenue, Madison, WI 53705, USA
    Chem Soc Rev 39:1036-48. 2010
    ..The goal is to guide new and interested researchers to the important areas and challenges facing the scientific community as we strive toward full integration of microfluidics with biology...
  63. pmc Selective and tunable gradient device for cell culture and chemotaxis study
    Dongshin Kim
    Department of Mechanical Engineering, University of Wisconsin, Madison, WI 53706, USA
    Lab Chip 9:1797-800. 2009
    ..Neutrophils were used to demonstrate the capability of the device for chemotaxis research. Experiments exhibited successful migration up a concentration gradient of interleukin 8...
  64. pmc Self-assembled peptide monolayers as a toxin sensing mechanism within arrayed microchannels
    Megan L Frisk
    Department of Chemistry, University of Wisconsin, Madison, Wisconsin 53706, USA
    Anal Chem 81:2760-7. 2009
    ..The modular design of this microfluidic SAM platform allows for extension to sensing other toxins that operate via enzymatic cleavage, such as the remaining BoNT serotypes B-G, anthrax, and tetanus toxin...
  65. pmc Microfluidics meet cell biology: bridging the gap by validation and application of microscale techniques for cell biological assays
    Amy L Paguirigan
    Department of Biomedical Engineering, University of Wisconsin, Madison, WI 53706, USA
    Bioessays 30:811-21. 2008
    ..With more validation and improved designs, microscale techniques hold immense promise as a platform to study aspects of cell biology that are not possible using current macroscale techniques...
  66. ncbi request reprint An externally driven magnetic microstirrer
    Glennys A Mensing
    Department of Biomedical Engineering, University of Wisconsin, Madison, WI 53706, USA
    Philos Transact A Math Phys Eng Sci 362:1059-68. 2004
    ..An analysis of mixing efficiency is based on greyscale intensity measurements of two coloured streams passing through the mixer. A brief hypothesis of the mechanism of mixing is also presented...
  67. pmc An accessible micro-capillary electrophoresis device using surface-tension-driven flow
    Swomitra K Mohanty
    Department of Biomedical Engineering, University of Wisconsin Madison, Madison, WI, USA
    Electrophoresis 30:1470-81. 2009
    ..g. micropipette, electrophoretic power supplies, and microscopes) already present in most labs for sample manipulation and detection, making it more accessible for potential end users...
  68. ncbi request reprint Diffusion dependent cell behavior in microenvironments
    Hongmei Yu
    Department of Biomedical Engineering, University of Wisconsin Madison, Madison WI 53706, USA
    Lab Chip 5:1089-95. 2005
    ..This study provides insights into the potential effects of the diffusion of soluble factors and related effects on cell behavior in microenvironments relevant to the emerging use of microchannel culture systems...
  69. pmc Control of 3-dimensional collagen matrix polymerization for reproducible human mammary fibroblast cell culture in microfluidic devices
    Kyung Eun Sung
    Department of Biomedical Engineering, University of Wisconsin, Madison, WI 53706, USA
    Biomaterials 30:4833-41. 2009
    ..In addition, the presented method lays the foundation for high throughput 3D cellular screening...
  70. pmc A quantitative comparison of human HT-1080 fibrosarcoma cells and primary human dermal fibroblasts identifies a 3D migration mechanism with properties unique to the transformed phenotype
    Michael P Schwartz
    Department of Biomedical Engineering, University of Wisconsin Madison, Madison, Wisconsin, United States of America
    PLoS ONE 8:e81689. 2013
    ..Based on our results, we propose that HT-1080s migrate in synthetic ECM with functional properties that are a direct consequence of their transformed phenotype. ..
  71. doi request reprint Cell-free protein expression in a microchannel array with passive pumping
    Ruba Khnouf
    Department of Biomedical Engineering, University of Florida, Gainesville, FL 32611, USA
    Lab Chip 9:56-61. 2009
    ....
  72. pmc One-step purification of nucleic acid for gene expression analysis via Immiscible Filtration Assisted by Surface Tension (IFAST)
    Scott M Berry
    Department of Biomedical Engineering, University of Wisconsin Madison, 1111 Highland Ave, Rm 6009, Madison, WI 53705, USA
    Lab Chip 11:1747-53. 2011
    ..The IFAST technology thus significantly enhances researchers' abilities to isolate and analyze nucleic acids, a process which is critical and ubiquitous in an extensive array of scientific fields...
  73. ncbi request reprint Protocol for the fabrication of enzymatically crosslinked gelatin microchannels for microfluidic cell culture
    Amy L Paguirigan
    Department of Biomedical Engineering, University of Wisconsin, Engineering Centers Building, 1150 Engineering Dr, Madison, Wisconsin 53706, USA
    Nat Protoc 2:1782-8. 2007
    ..Using these devices, the effects of both the extracellular matrix and soluble factors on cellular behavior and differentiation can be studied in microenvironments that more closely mimic the in vivo environment...
  74. ncbi request reprint Characterization of a membrane-based gradient generator for use in cell-signaling studies
    Vinay V Abhyankar
    Department of Biomedical Engineering, University of Wisconsin Madison, Madison, WI 53706, USA
    Lab Chip 6:389-93. 2006
    ..We present the theory, design, and characterization of this device and provide an example of neutrophil chemotaxis as proof of concept for future quantitative cell-signaling applications...
  75. ncbi request reprint Magnetically-driven biomimetic micro pumping using vortices
    Javier Atencia
    Biomedical Engineering Department, University of Wisconsin Madison, Madison, WI 53706, USA
    Lab Chip 4:598-602. 2004
    ..Experiments are presented characterizing the pumps and their flow patterns. The range of operation of the pumps is from 3 microl min(-1) to 600 microl min(-1), though smaller flow rates are also possible...
  76. ncbi request reprint Controlled microfluidic interfaces
    Javier Atencia
    Department of Biomedical Engineering, University of Wisconsin Madison, 1550 Engineering Drive, Rm 2142 ECB, Madison, Wisconsin WI 53706, USA
    Nature 437:648-55. 2005
    ....
  77. ncbi request reprint A plate reader-compatible microchannel array for cell biology assays
    Hongmei Yu
    Department of Biomedical Engineering, Madison, WI 53706, USA
    Lab Chip 7:388-91. 2007
    ..The simplicity and flexibility of the platform, combined with the throughput of automated detection systems, will facilitate the adoption of microfluidic culture systems in biological laboratories...
  78. ncbi request reprint In situ fabricated porous filters for microsystems
    Jaisree Moorthy
    Department of Biomedical Engineering, University of Wisconsin at Madison, WI 53706, USA
    Lab Chip 3:62-6. 2003
    ..The porous filter thus mimics the functionality of the centrifuge, with the added benefits of no power requirements and ability to handle small sample volumes...
  79. ncbi request reprint Flow control with hydrogels
    David T Eddington
    Department of Biomedical Engineering, University of Wisconsin Madison, 1550 Engineering Dr, Madison, WI 53706, USA
    Adv Drug Deliv Rev 56:199-210. 2004
    ..Therefore, the door to physiological feedback controlling the infusion rate in a drug delivery device is opened and has the potential to revolutionize protein pharmaceutical drug delivery...
  80. ncbi request reprint Microfluidic tectonics platform: A colorimetric, disposable botulinum toxin enzyme-linked immunosorbent assay system
    Jaisree Moorthy
    Department of Biomedical Engineering, University of Wisconsin, 1550 Engineering Drive, Madison, WI 53706, USA
    Electrophoresis 25:1705-13. 2004
    ..In addition, external materials such as membranes can be incorporated into the device as components. Individual components that were necessary to perform these steps were characterized, and their mutual compatibility is also discussed...
  81. pmc Biological implications of polydimethylsiloxane-based microfluidic cell culture
    Keil J Regehr
    Department of Biomedical Engineering, University of Wisconsin Madison, Madison, WI 53706 1609, USA
    Lab Chip 9:2132-9. 2009
    ..These findings highlight the importance of careful consideration of culture system properties when determining an appropriate environment for biological experiments...
  82. pmc Polarity effect in electrovibration for tactile display
    Kurt A Kaczmarek
    Department of Orthopedics and Rehabilitation Medicine, University of Wisconsin, Madison, WI 53706, USA
    IEEE Trans Biomed Eng 53:2047-54. 2006
    ..We furthermore propose using negative pulses for insulated tactile displays based on electrovibration because their sensory thresholds were found to be more stable than for waveforms incorporating positive pulses...
  83. ncbi request reprint An oral tactile interface for blind navigation
    Hui Tang
    Department of Biomedical Engineering, University of Wisconsin Madison, USA
    IEEE Trans Neural Syst Rehabil Eng 14:116-23. 2006
    ..Furthermore, identification of leftward or rightward movements was highly accurate while performance on forward or backward moving patterns was mixed and varied considerably among subjects...
  84. ncbi request reprint Dissolvable membranes as sensing elements for microfluidics based biological/chemical sensors
    Sudheer S Sridharamurthy
    Department of Electrical and Computer Engineering, University of Wisconsin Madison, 1415 Engineering Drive, Madison, WI 53706, USA
    Lab Chip 6:840-2. 2006
    ..A range of targets could be sensed by defining membranes specific to the target. This sensing mechanism might find applications in sensing targets such as toxins, which exhibit enzymatic activity...
  85. ncbi request reprint Microscale bioengineering inspired by nature: from widgets to cell biology
    David J Beebe
    Biomedical Engineering, University of Wisconsin Madison, USA
    Conf Proc IEEE Eng Med Biol Soc 2007:1. 2007
  86. pmc An adaptable hydrogel array format for 3-dimensional cell culture and analysis
    Leenaporn Jongpaiboonkit
    Department of Biomedical Engineering, University of Wisconsin, Madison, WI 53706, United States
    Biomaterials 29:3346-56. 2008
    ..Taken together, our results indicate that the adaptable, array-based format developed in this study may be useful as an enhanced throughput platform for 3-D culture of a variety of cell types...
  87. ncbi request reprint Microfluidic logic gates and timers
    Michael W Toepke
    Department of Biomedical Engineering, University of Wisconsin Madison, WI 53706, USA
    Lab Chip 7:1449-53. 2007
    ..The components can be implemented with standard pipettes and provide a means of non-electronic and autonomous preprogrammed control with potential utility in cell studies and high throughput screening applications...
  88. ncbi request reprint Spatiotemporal micropatterning of cells on arbitrary substrates
    Vinay V Abhyankar
    Department of Biomedical Engineering, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    Anal Chem 79:4066-73. 2007
    ..Spatiotemporal patterning provides a foundation for future biological studies that explore the time-dependent relationships between cell-cell signaling and cellular responses...
  89. ncbi request reprint Automated cell culture in high density tubeless microfluidic device arrays
    Ivar Meyvantsson
    Department of Biomedical Engineering, University of Wisconsin Madison, 1550 Engineering Drive, Madison, WI 53706, USA
    Lab Chip 8:717-24. 2008
    ....
  90. ncbi request reprint High-throughput microfluidics: improved sample treatment and washing over standard wells
    Jay Warrick
    Department of Biomedical Engineering, University of Wisconsin Madison, Madison, W 53706, USA
    Lab Chip 7:316-21. 2007
    ..The advantages of microchannels outlined here can have large-scale effects on cost and accuracy in screening applications...
  91. ncbi request reprint Autonomously-triggered microfluidic cooling using thermo-responsive hydrogels
    Abhishek K Agarwal
    Department of Electrical and Computer Engineering, University of Wisconsin Madison, Madison, WI 53706, USA
    Lab Chip 7:310-5. 2007
    ..Furthermore, the effect of magnetic stirrer frequency on the fluid cooling and flowrates for different two-blade nickel impeller designs are presented...
  92. ncbi request reprint Understanding microchannel culture: parameters involved in soluble factor signaling
    Hongmei Yu
    Department of Biomedical Engineering, University of Wisconsin Madison, Madison, WI 53706, USA
    Lab Chip 7:726-30. 2007
    ..The convective-free microchannel environment may prove useful for studying soluble factor signaling in vitro, and to test models and predictions of autocrine-paracrine signaling...
  93. ncbi request reprint PDMS absorption of small molecules and consequences in microfluidic applications
    Michael W Toepke
    Department of Biomedical Engineering, University of Wisconsin Madison, Madison, WI 53706, USA
    Lab Chip 6:1484-6. 2006
    ..Partitioning of molecules into PDMS can significantly change solution concentrations and could potentially alter experimental outcomes. Herein we discuss PDMS absorption and its potential impact on microfluidic experiments...
  94. ncbi request reprint Adaptive liquid microlenses activated by stimuli-responsive hydrogels
    Liang Dong
    Department of Electrical and Computer Engineering, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    Nature 442:551-4. 2006
    ....
  95. ncbi request reprint Hydrodynamic microfabrication via"on the fly" photopolymerization of microscale fibers and tubes
    Wonje Jeong
    Department of Biomedical Engineering, Dankook University, San 29, Anseodong Cheonan Chungnam, South Korea
    Lab Chip 4:576-80. 2004
    ..Responsive woven fabric and biosensing fibers are demonstrated. The fabrication process is simple, cost effective and flexible in materials, geometries, and scales...
  96. ncbi request reprint Principles of surface-directed liquid flow in microfluidic channels
    Bin Zhao
    The Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana Champaign, Urbana 61801, USA
    Anal Chem 74:4259-68. 2002
    ..Experimental results are in good agreement with the analytical predictions...
  97. ncbi request reprint Characterization of a silicon-based shear-force sensor on human subjects
    Lin Wang
    Department of Electrical and Computer Engineering, The Beckman Institute for Advanced Science and Technology, University of Illinois, Urbana, IL 61801, USA
    IEEE Trans Biomed Eng 49:1340-7. 2002
    ..Force measurement via a tracking experiment is evaluated at four shear (9, 18, 25, and 35 N) and three compressive (7, 15, and 26 N) force levels. The sensor has good repeatability (SD approximately 1.7 N) with an average error of 12.1%...
  98. pmc Managing evaporation for more robust microscale assays. Part 1. Volume loss in high throughput assays
    Erwin Berthier
    MacDiarmid Institute for Advanced Materials and Nanotechnology, Department of Electrical and Computer Engineering, University of Canterbury, Christchurch, NZ
    Lab Chip 8:852-9. 2008
    ..Ev can be used both as a design tool and as an analysis parameter. The advantageous use of evaporation in some applications is also discussed...
  99. ncbi request reprint Organic and biomimetic designs for microfluidic systems
    Jaisree Moorthy
    University of Wisconsin Madison, USA
    Anal Chem 75:292A-301A. 2003
  100. ncbi request reprint Microenvironment design considerations for cellular scale studies
    Glenn M Walker
    Department of Molecular Physiology and Biophysics, Vanderbuilt Institute for Integrative Biosystem Research and Education, Vanderbuilt University, Nashville, TN 37232, USA
    Lab Chip 4:91-7. 2004
    ..Current research using microtechnology to investigate in vitro cellular microenvironments is presented and areas where more research is needed in characterizing the in vitro microenvironment are outlined...
  101. ncbi request reprint Flow rate analysis of a surface tension driven passive micropump
    Erwin Berthier
    MacDiarmid Institute for Advanced Materials and Nanotechnology, Department of Electrical and Computer Engineering, University of Canterbury, Christchurch, NZ
    Lab Chip 7:1475-8. 2007
    ..We find that during the first phase the flow rate is stable and that this phase can be prolonged by refilling the inlet drop to produce continuous flow in the microchannel...

Research Grants4

  1. Microfluidic Channels for High Density, High Performance Culture Assays
    David Beebe; Fiscal Year: 2007
    ..As part of these studies, we will demonstrate a 25X reduction in the number of animals required for typical 24 well plate cell-based studies. ..
  2. Study of Progenitor Regulation in Mouse Mammary Gland
    David Beebe; Fiscal Year: 2007
    ..Such a model would have a significant impact on breast cancer research. ..