Brett M Barney
Affiliation: University of Minnesota
Knutson C, Plunkett M, Liming R, Barney B. Efforts toward optimization of aerobic biohydrogen reveal details of secondary regulation of biological nitrogen fixation by nitrogenous compounds in Azotobacter vinelandii. Appl Microbiol Biotechnol. 2018;102:10315-10325 pubmed publisher
..These results provide essential details to inform future biosynthetic approaches to yield nitrogen products that do not inadvertently inhibit BNF. ..
Barney B, Wahlen B, Garner E, Wei J, Seefeldt L. Differences in substrate specificities of five bacterial wax ester synthases. Appl Environ Microbiol. 2012;78:5734-45 pubmed publisher
..The results provide a framework to test prospective differences between these enzymes for potential biotechnological applications such as high-value petrochemicals and biofuel production. ..
Barney B, Eberhart L, Ohlert J, Knutson C, Plunkett M. Gene Deletions Resulting in Increased Nitrogen Release by Azotobacter vinelandii: Application of a Novel Nitrogen Biosensor. Appl Environ Microbiol. 2015;81:4316-28 pubmed publisher
..These results reveal alternative approaches that can be used in various combinations to yield new strains that might have further application in biofertilizer schemes. ..
Barney B, Ohlert J, Timler J, Lijewski A. Altering small and medium alcohol selectivity in the wax ester synthase. Appl Microbiol Biotechnol. 2015;99:9675-84 pubmed publisher
..This analysis provides evidence of residues that line the binding site for wax ester synthase, which will aid rational approaches to improve this enzyme with specific substrates. ..
Bertram J, Mulliner K, Shi K, Plunkett M, Nixon P, Serratore N, et al
. Five Fatty Aldehyde Dehydrogenase Enzymes from Marinobacter and Acinetobacter spp. and Structural Insights into the Aldehyde Binding Pocket. Appl Environ Microbiol. 2017;83: pubmed publisher
Barney B, Plunkett M, Natarajan V, Mus F, Knutson C, Peters J. Transcriptional Analysis of an Ammonium-Excreting Strain of Azotobacter vinelandii Deregulated for Nitrogen Fixation. Appl Environ Microbiol. 2017;83: pubmed publisher
..These results should inform future studies aimed at elucidating the important features of this phenotype and at maximizing ammonium production by this strain. ..