Stephen Barnes

Summary

Affiliation: University of Alabama at Birmingham
Country: USA

Publications

  1. pmc Isoflavones and PPAR Signaling: A Critical Target in Cardiovascular, Metastatic, and Metabolic Disease
    Rakesh P Patel
    Department of Pathology, University of Alabama at Birmingham, 1918 University Boulevard, Birmingham, AL 35294, USA
    PPAR Res 2010:153252. 2010
  2. pmc In nutrition, can we "see" what is good for us?
    Stephen Barnes
    Department of Pharmacology and Toxicology, Targeted Metabolomics and Proteomics Laboratory, University of Alabama at Birmingham, Birmingham, AL, USA
    Adv Nutr 4:327S-34S. 2013
  3. pmc Sources of variation in Affymetrix microarray experiments
    Stanislav O Zakharkin
    University of Alabama, Birmingham, Alabama, USA
    BMC Bioinformatics 6:214. 2005
  4. ncbi request reprint In the postgenomic era, can high-dimensional analyses deliver the answers we're looking for?
    Stephen Barnes
    Department of Pharmacology and Toxicology, UAB Center for Nutrient Gene Interaction in Cancer Prevention, Comprehensive Cancer Center, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    J Nutr 136:2709S-10S. 2006
  5. ncbi request reprint Applications of LC-MS in the study of the uptake, distribution, metabolism and excretion of bioactive polyphenols from dietary supplements
    Stephen Barnes
    Department of Pharmacology and Toxicology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    Life Sci 78:2054-9. 2006
  6. ncbi request reprint Current progress in the use of traditional medicines and nutraceuticals
    Stephen Barnes
    Department of Pharmacology and Toxicology, McCallum Research Building, University of Alabama at Birmingham, 1918 University Boulevard, Birmingham, Alabama 35294, USA
    Curr Opin Plant Biol 8:324-8. 2005
  7. pmc Technologies and experimental approaches at the National Institutes of Health Botanical Research Centers
    Stephen Barnes
    Botanical Center for Age Related Disease, Purdue University University of Alabama at Birmingham, West Lafayette, IN, USA
    Am J Clin Nutr 87:476S-80S. 2008
  8. ncbi request reprint Nutriproteomics: identifying the molecular targets of nutritive and non-nutritive components of the diet
    Stephen Barnes
    Departments of Pharmacology and Toxicology, 452 McCallum Building, University of Alabama at Birmingham, 1918 University Boulevard, Birmingham, AL 35294, USA
    J Biochem Mol Biol 37:59-74. 2004
  9. pmc High-resolution mass spectrometry analysis of protein oxidations and resultant loss of function
    Stephen Barnes
    Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    Biochem Soc Trans 36:1037-44. 2008
  10. pmc Nutritional genomics, polyphenols, diets, and their impact on dietetics
    Stephen Barnes
    Department of Pharmacology and Toxicology, 452 McCallum Research Building, University of Alabama at Birmingham, 1918 University Blvd, Birmingham, AL 35294, USA
    J Am Diet Assoc 108:1888-95. 2008

Research Grants

  1. 9.4 Tesla Fourier Transform Mass Spectrometer
    Stephen Barnes; Fiscal Year: 2002
  2. A sensitive triple quadrupole mass spectrometer
    Stephen Barnes; Fiscal Year: 2004
  3. AMINO ACID CONJUGATION OF BILE ACIDS
    Stephen Barnes; Fiscal Year: 2006
  4. Center for Nutrient-Gene Interaction in Cancer Prevention
    Stephen Barnes; Fiscal Year: 2007

Collaborators

Detail Information

Publications90

  1. pmc Isoflavones and PPAR Signaling: A Critical Target in Cardiovascular, Metastatic, and Metabolic Disease
    Rakesh P Patel
    Department of Pathology, University of Alabama at Birmingham, 1918 University Boulevard, Birmingham, AL 35294, USA
    PPAR Res 2010:153252. 2010
    ..Future studies will involve careful identification of the underlying mechanisms whereby isoflavones have their action via PPAR signaling...
  2. pmc In nutrition, can we "see" what is good for us?
    Stephen Barnes
    Department of Pharmacology and Toxicology, Targeted Metabolomics and Proteomics Laboratory, University of Alabama at Birmingham, Birmingham, AL, USA
    Adv Nutr 4:327S-34S. 2013
    ..Extensive metabolism by phase I/II enzymes and by the gut microbiome may also create compounds that the eye is never allowed to appreciate...
  3. pmc Sources of variation in Affymetrix microarray experiments
    Stanislav O Zakharkin
    University of Alabama, Birmingham, Alabama, USA
    BMC Bioinformatics 6:214. 2005
    ..The objectives of this study were: (1) to estimate relative magnitudes of different sources of variation and (2) to evaluate agreement between biological and technical replicates...
  4. ncbi request reprint In the postgenomic era, can high-dimensional analyses deliver the answers we're looking for?
    Stephen Barnes
    Department of Pharmacology and Toxicology, UAB Center for Nutrient Gene Interaction in Cancer Prevention, Comprehensive Cancer Center, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    J Nutr 136:2709S-10S. 2006
  5. ncbi request reprint Applications of LC-MS in the study of the uptake, distribution, metabolism and excretion of bioactive polyphenols from dietary supplements
    Stephen Barnes
    Department of Pharmacology and Toxicology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    Life Sci 78:2054-9. 2006
    ..In this paper, applications of these methods are described for the analysis of isoflavones, salvinorin A, synephrine isomers and their metabolites in serum, urine and aqueous humor...
  6. ncbi request reprint Current progress in the use of traditional medicines and nutraceuticals
    Stephen Barnes
    Department of Pharmacology and Toxicology, McCallum Research Building, University of Alabama at Birmingham, 1918 University Boulevard, Birmingham, Alabama 35294, USA
    Curr Opin Plant Biol 8:324-8. 2005
    ....
  7. pmc Technologies and experimental approaches at the National Institutes of Health Botanical Research Centers
    Stephen Barnes
    Botanical Center for Age Related Disease, Purdue University University of Alabama at Birmingham, West Lafayette, IN, USA
    Am J Clin Nutr 87:476S-80S. 2008
    ..In summary, the skills needed to carry out botanical centers' research are extensive and may exceed those practiced by most NIH investigators...
  8. ncbi request reprint Nutriproteomics: identifying the molecular targets of nutritive and non-nutritive components of the diet
    Stephen Barnes
    Departments of Pharmacology and Toxicology, 452 McCallum Building, University of Alabama at Birmingham, 1918 University Boulevard, Birmingham, AL 35294, USA
    J Biochem Mol Biol 37:59-74. 2004
    ..These are described, as are the pros and cons of each method in current use...
  9. pmc High-resolution mass spectrometry analysis of protein oxidations and resultant loss of function
    Stephen Barnes
    Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    Biochem Soc Trans 36:1037-44. 2008
    ..These results suggest that future in vivo studies should carefully assess the critical sites that are modified rather than using antibodies that do not distinguish between different modified sites...
  10. pmc Nutritional genomics, polyphenols, diets, and their impact on dietetics
    Stephen Barnes
    Department of Pharmacology and Toxicology, 452 McCallum Research Building, University of Alabama at Birmingham, 1918 University Blvd, Birmingham, AL 35294, USA
    J Am Diet Assoc 108:1888-95. 2008
    ..These challenges are explored within the context of soy intake. Finally, the importance of international cooperation in nutritional genomics research is presented...
  11. ncbi request reprint Soy isoflavones--phytoestrogens and what else?
    Stephen Barnes
    Department of Pharmacology and Toxicology, Center for Nutrient Gene Interaction, University of Alabama at Birmingham, and Purdue University University of Alabama at Birmingham Botanicals Center for Age Related Disease, 35294, USA
    J Nutr 134:1225S-1228S. 2004
    ..In another approach using affinity chromatography methods, DING, a novel genistein-binding protein of yet unknown function, was isolated from human breast cancer MCF-7 cells. Its function remains to be established...
  12. pmc The metabolism and analysis of isoflavones and other dietary polyphenols in foods and biological systems
    Stephen Barnes
    Department of Pharmacology and Toxicology, MCLM 452, University of Alabama at Birmingham, 1918 University Boulevard, Birmingham, AL 35294, USA
    Food Funct 2:235-44. 2011
    ..This permits the study of the penetration of the polyphenols into the interstitial fluid, the fluid that is actually in contact with non-vascular cells...
  13. ncbi request reprint Phyto-oestrogens and osteoporosis: what is a safe dose?
    Stephen Barnes
    Department of Pharmacology and Toxicology, and Comprehensive Cancer Center, University of Alabama at Birmingham, 35294, USA
    Br J Nutr 89:S101-8. 2003
    ..Whether these doses are sufficient to prevent osteoporosis is a separate matter...
  14. pmc The biochemistry, chemistry and physiology of the isoflavones in soybeans and their food products
    Stephen Barnes
    Department of Pharmacology and Toxicology, University of Alabama at Birmingham and Purdue University University of Alabama at Birmingham Botanicals Center for Age Related Disease, Birmingham, Alabama 35294, USA
    Lymphat Res Biol 8:89-98. 2010
    ..These various properties may explain the much lower incidence of hormonally-dependent breast cancer in Asian populations compared to Americans and Europeans...
  15. pmc Cautions and research needs identified at the equol, soy, and menopause research leadership conference
    Stephen Barnes
    Department of Pharmacology and Toxicology, and Center for Nutrient Gene Interaction, University of Alabama at Birmingham and Purdue University University of Alabama at Birmingham Botanicals Research Center for Age Related Disease, Birmingham, AL 35294, USA
    J Nutr 140:1390S-4S. 2010
    ....
  16. ncbi request reprint Introduction and satellite session at the fourth International Symposium on the Role of Soy in Preventing and Treating Chronic Disease
    Stephen Barnes
    Department of Pharmacology and Toxicology, University of Alabama at Birmingham, AL 35294, USA
    J Nutr 132:545S-546S. 2002
  17. ncbi request reprint Neutrophil myeloperoxidase chlorinates and nitrates soy isoflavones and enhances their antioxidant properties
    Brenda J Boersma
    Department of Pharmacology and Toxicology, University of Alabama at Birmingham, Birmingham, AL 35294 2180, USA
    Free Radic Biol Med 35:1417-30. 2003
    ..These results lead to the hypothesis that inflammatory cell-specific metabolism of polyphenolics can modify the properties of these compounds at the local site of inflammation...
  18. ncbi request reprint Bile acid coenzyme A: amino acid N-acyltransferase in the amino acid conjugation of bile acids
    Erin M Shonsey
    Department of Pharmacology and Toxicology, University of Alabama, Birmingham, USA
    Methods Enzymol 400:374-94. 2005
    ..In addition, an enzyme that catalyzes further metabolism of glycine-conjugated bile acids is described...
  19. pmc Genistein, a phytoestrogen, improves total cholesterol, and Synergy, a prebiotic, improves calcium utilization, but there were no synergistic effects
    Leecole L Legette
    Department of Foods and Nutrition, Purdue University, West Lafayette, IN, USA
    Menopause 18:923-31. 2011
    ..The aim of this study was to determine the effect of both a prebiotic (Synergy) and a phytoestrogen (genistein) on bone and blood lipid levels in an animal model of postmenopausal women...
  20. ncbi request reprint 2D gel proteomics: an approach to study age-related differences in protein abundance or isoform complexity in biological samples
    Helen Kim
    Department of Pharmacology and Toxicology, University of Alabama at Birmingham, Birmingham, AL, USA
    Methods Mol Biol 371:349-91. 2007
    ....
  21. ncbi request reprint Metabolism of phytoestrogen conjugates
    Tracy L D'Alessandro
    Department of Pharmacology and Toxicology, University of Alabama at Birmingham, USA
    Methods Enzymol 400:316-42. 2005
    ..This chapter describes the methods of analysis of all these compounds, the sources of or methods to manufacture suitable standards, and the procedures for examining the enzymes that catalyze these reactions...
  22. pmc Chronic dietary kudzu isoflavones improve components of metabolic syndrome in stroke-prone spontaneously hypertensive rats
    Ning Peng
    Department of Cell Biology, University of Alabama at Birmingham, Birmingham, Alabama 35294 0006, USA
    J Agric Food Chem 57:7268-73. 2009
    ..These results indicate that long-term dietary kudzu root extract supplementation can improve glucose, lipid, and blood pressure control in intact and ovariectomized SP-SHR...
  23. doi request reprint Liquid chromatography tandem mass spectrometry identification of proanthocyanidins in rat plasma after oral administration of grape seed extract
    Jeevan K Prasain
    Department of Pharmacology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    Phytomedicine 16:233-43. 2009
    ..Using LC-MS/MS, (+)-catechin and (-)-epicatechin were identified in the brain conclusively. These data suggested that GSE catechins cross the blood brain barrier and may be responsible for the neuroprotective effects of GSE...
  24. ncbi request reprint Proteomics analysis of the actions of grape seed extract in rat brain: technological and biological implications for the study of the actions of psychoactive compounds
    Helen Kim
    Department of Pharmacology and Toxicology, University of Alabama at Birmingham, AL 35294, USA
    Life Sci 78:2060-5. 2006
    ..The fact that GSE affects proteins implicated in cognitive disorders suggests moreover that GSE may have impact on the actions of psychoactive drugs by maintaining an overall viability of the nervous system...
  25. pmc Curcumin reverses breast tumor exosomes mediated immune suppression of NK cell tumor cytotoxicity
    Huang Ge Zhang
    Division of Clinical Immunology and Rheumatology, Department of Medicine, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    Biochim Biophys Acta 1773:1116-23. 2007
    ..These data suggest that partial reversal of tumor exosome-mediated inhibition of NK cell tumor cytotoxicity may account for the anti-cancer properties of curcumin...
  26. ncbi request reprint Polyphenols, inflammatory response, and cancer prevention: chlorination of isoflavones by human neutrophils
    Tracy D'Alessandro
    Department of Pharmacology and Toxicology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    J Nutr 133:3773S-3777S. 2003
    ..These data imply a potential role for modified forms of genistein that would be produced in the inflammatory environment in and around a tumor...
  27. doi request reprint Tissue distribution of puerarin and its conjugated metabolites in rats assessed by liquid chromatography-tandem mass spectrometry
    Jeevan K Prasain
    Department of Pharmacology and Toxicology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    Phytomedicine 16:65-71. 2009
    ..This report identifying puerarin in several organs including kidney and pancreas may explain its beneficial effects in diabetes...
  28. ncbi request reprint active site modifications of the brain isoform of creatine kinase by 4-hydroxy-2-nonenal correlate with reduced enzyme activity: mapping of modified sites by Fourier transform-ion cyclotron resonance mass spectrometry
    Shannon M Eliuk
    Department of Pharmacology Toxicology, UAB Biomedical FT ICR MS Laboratory, University of Alabama at Birmingham, 1918 University Boulevard, Birmingham, LA 35294, USA
    Chem Res Toxicol 20:1260-8. 2007
    ....
  29. ncbi request reprint Anti-inflammatory effects of isoflavones are dependent on flow and human endothelial cell PPARgamma
    Balu K Chacko
    Department of Pathology, Purdue UAB Botanical Center, University of Alabama, Birmingham, AL 35294, USA
    J Nutr 137:351-6. 2007
    ....
  30. ncbi request reprint Comparative effect of soy protein, soy isoflavones, and 17beta-estradiol on bone metabolism in adult ovariectomized rats
    David J Cai
    Department of Foods and Nutrition, Purdue University, West Lafayette, Indiana 47907, USA
    J Bone Miner Res 20:828-39. 2005
    ..Results confirmed the bone-preserving effect of estrogen but did not support a bone-sparing role of soy isoflavones...
  31. ncbi request reprint In vivo detection of secreted proteins from wounded skin using capillary ultrafiltration probes and mass spectrometric proteomics
    Chun Ming Huang
    Department of Dermatology, University of Alabama at Birmingham, Birmingham, AL, USA
    Proteomics 6:5805-14. 2006
    ..The technique of using CUF probes linked to mass spectrometric proteomics represents a powerful method to identify in vivo secreted proteins, and may be applicable for identification of proteins relevant in various human diseases...
  32. ncbi request reprint Modification of Cytochrome c by 4-hydroxy- 2-nonenal: evidence for histidine, lysine, and arginine-aldehyde adducts
    Amanda L Isom
    Department of Pharmacology and Toxicology, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    J Am Soc Mass Spectrom 15:1136-47. 2004
    ....
  33. ncbi request reprint Proteomics analysis of rat brain protein modulations by grape seed extract
    Jessy Deshane
    Department of Pharmacology and Toxicology, University of Alabama at Birmingham Comprehensive Cancer Center, Birmingham, Alabama 35294, USA
    J Agric Food Chem 52:7872-83. 2004
    ..This is the first identification and quantitation of specific proteins in mammalian tissues modulated by a dietary supplement, as well as the first to demonstrate links of such proteins with any disease...
  34. pmc Genistein and genistein-containing dietary supplements accelerate the early stages of cataractogenesis in the male ICR/f rat
    Kyle A Floyd
    Department of Pharmacology and Toxicology, University of Alabama at Birmingham, L108 Volker Hall, 1670 University Blvd, Birmingham, AL 35294, USA
    Exp Eye Res 92:120-7. 2011
    ..These data suggest that genistein-containing dietary supplements accelerate the early stages of cataractogenesis in the male ICR/f rat, with no dose-dependent effects...
  35. pmc Cardiac hypertrophy in mice with long-chain acyl-CoA dehydrogenase or very long-chain acyl-CoA dehydrogenase deficiency
    Keith B Cox
    Department of Genetics, University of Alabama at Birmingham, USA
    Lab Invest 89:1348-54. 2009
    ....
  36. ncbi request reprint A sensitive method for the quantitative measurement of protein thiol modification in response to oxidative stress
    Aimee Landar
    Department of Pathology, Center for Free Radical Biology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    Free Radic Biol Med 40:459-68. 2006
    ..Thus, we have developed a versatile, cost-effective standard that can be used in proteomic methods to quantitate biotin tags in response to oxidative stress...
  37. ncbi request reprint In vivo protein sampling using capillary ultrafiltration semi-permeable hollow fiber and protein identification via mass spectrometry-based proteomics
    Chun Ming Huang
    Department of Dermatology, University of Alabama at Birmingham, VH 566A, 1670 University Blvd, Birmingham, AL 35294 0019, USA
    J Chromatogr A 1109:144-51. 2006
    ..Also, the technique represents a powerful method for the identification of in vivo secreted proteins and has potential application for in the detection of biomarkers for human diseases...
  38. ncbi request reprint Mass spectrometric proteomics profiles of in vivo tumor secretomes: capillary ultrafiltration sampling of regressive tumor masses
    Chun Ming Huang
    Department of Dermatology, University of Alabama at Birmingham, Birmingham, AL, USA
    Proteomics 6:6107-16. 2006
    ..The technique involving CUF probes linked to mass spectrometric proteomics reinforces systems biology studies of cell-cell interactions and is potentially applicable to the discovery of in vivo biomarkers in human disease...
  39. pmc Determination of cranberry phenolic metabolites in rats by liquid chromatography-tandem mass spectrometry
    Rajani Rajbhandari
    Department of Pharmacology and Toxicology, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    J Agric Food Chem 59:6682-8. 2011
    ..These results demonstrate that cranberry components undergo rapid metabolism and elimination into the urine of rats and are present in the urinary bladder tissue potentially allowing them to inhibit urinary bladder carcinogenesis...
  40. ncbi request reprint Quantification and regulation of the subcellular distribution of bile acid coenzyme A:amino acid N-acyltransferase activity in rat liver
    Nathan A Styles
    Department of Pharmacology and Toxicology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    J Lipid Res 48:1305-15. 2007
    ..Approximately 15% of BAT activity is present in the peroxisomal matrix. These data support the novel finding that clofibrate treatment does not directly regulate BAT activity but does alter the subcellular localization of BAT...
  41. ncbi request reprint Chemoprevention by grape seed extract and genistein in carcinogen-induced mammary cancer in rats is diet dependent
    Helen Kim
    Departments of Pharmacology and Toxicology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    J Nutr 134:3445S-3452S. 2004
    ....
  42. ncbi request reprint Identification of isoflavone glycosides in Pueraria lobata cultures by tandem mass spectrometry
    Jeevan K Prasain
    Department of Pharmacology and Toxicology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    Phytochem Anal 18:50-9. 2007
    ..The molecular weights, interpretation of characteristic fragment ions obtained from HPLC-MS/MS and comparison with reported data allowed the putative identification of the isoflavone metabolites...
  43. pmc UV-A-induced structural and functional changes in human lens deamidated alphaB-crystallin
    Kerri Mafia
    Department of Pharmacology, University of Alabama at Birmingham, Birmingham, AL 35226, USA
    Mol Vis 14:234-48. 2008
    ....
  44. ncbi request reprint Synthesis of bile acid coenzyme a thioesters in the amino acid conjugation of bile acids
    Erin M Shonsey
    Department of Pharmacology and Toxicology, University of Alabama, Birmingham, USA
    Methods Enzymol 400:360-73. 2005
    ....
  45. pmc Supplemental dietary racemic equol has modest benefits to bone but has mild uterotropic activity in ovariectomized rats
    Leecole L Legette
    Department of Foods and Nutrition, Purdue University, West Lafayette, IN 47907, USA
    J Nutr 139:1908-13. 2009
    ..Dietary equol had no stimulatory effect on mammary gland epithelium. We conclude that in OVX rats, a dietary equol dose that had modest effect on bone also exerts mild uterotropic effects...
  46. pmc A proposed metric for assessing the measurement quality of individual microarrays
    Kyoungmi Kim
    Department of Biostatistics, University of Alabama, Birmingham, Alabama, USA
    BMC Bioinformatics 7:35. 2006
    ..In some cases this may be manifested as systematic spatial variation on the surface of microarray in which expression measurements within an individual array may vary as a function of geographic position on the array surface...
  47. pmc Tissue localization and solubilities of αA-crystallin and its numerous C-terminal truncation products in pre- and postcataractous ICR/f rat lenses
    David R Stella
    Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, Alabama, USA
    Invest Ophthalmol Vis Sci 51:5153-61. 2010
    ..To investigate the tissue distribution and solubilities of various αA-crystallin truncation products in the cataractous ICR/f rat model...
  48. doi request reprint 2D difference gel electrophoresis of prepubertal and pubertal rat mammary gland proteomes
    Helen Kim
    Departments of Pharmacology and Toxicology, the UAB Center for Nutrient Gene Interaction, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    J Proteome Res 7:4638-50. 2008
    ..This is a first report of mammary gland proteome differences at these important breast cancer-relevant time-points...
  49. ncbi request reprint Review of the methods used in the determination of phytoestrogens
    Chao Cheng Wang
    Department of Pharmacology and Toxicology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    J Chromatogr B Analyt Technol Biomed Life Sci 777:3-28. 2002
    ..These methods are compared with other methods of phytoestrogen analysis utilizing immunoassay approaches. The advantages and disadvantages of each of these methods are highlighted and potential areas for further development identified...
  50. ncbi request reprint Inulin effects on bioavailability of soy isoflavones and their calcium absorption enhancing ability
    Tasleem A Zafar
    Department of Foods and Nutrition, Purdue University, West Lafayette, Indiana 47907, USA
    J Agric Food Chem 52:2827-31. 2004
    ..01) when inulin was added to the diet containing isoflavones. We conclude that isoflavones enhance calcium absorption, without synergy from inulin, and that inulin decreases equol production...
  51. ncbi request reprint Mass spectrometric methods for the analysis of chlorinated and nitrated isoflavonoids: a novel class of biological metabolites
    Jeevan K Prasain
    Department of Pharmacology and Toxicology, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    J Mass Spectrom 38:764-71. 2003
    ..This work provides a suitable analytical basis to aid the characterization of chlorinated and nitrated metabolites in studies in vivo and in vitro...
  52. ncbi request reprint Pharmacokinetic study of puerarin in rat serum by liquid chromatography tandem mass spectrometry
    Jeevan K Prasain
    Department of Pharmacology and Toxicology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    Biomed Chromatogr 21:410-4. 2007
    ..54 +/- 2.03 mg/L), T(max) (0.68 +/- 0.37 h), AUC(0-t) (7.29 +/- 3.79 mg h/L), AUC(0-infinity) (9.17 +/- 4.87 mg h/L), T(1/2) (1.7 +/- 0.6 h), CL/F (7.24 +/- 4.27 L/h/kg) and V/F (17.88 +/- 13.55 L/h/kg)...
  53. ncbi request reprint Contrasting effects of puerarin and daidzin on glucose homeostasis in mice
    Elias Meezan
    Department of Pharmacology and Toxicology, Comprehensive Cancer Center Mass Spectrometry Shared Facility, and Purdue UAB Botanicals Center for Age Related Diseases, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    J Agric Food Chem 53:8760-7. 2005
    ..The opposing effects of puerarin and daidzin on glucose homeostasis may have implications for the activity of dietary supplements that contain both of these isoflavonoids...
  54. ncbi request reprint Surfactant sodium lauryl sulfate enhances skin vaccination: molecular characterization via a novel technique using ultrafiltration capillaries and mass spectrometric proteomics
    Chun Ming Huang
    Department of Dermatology, Skin Diseases Research Center, University of Alabama, Birmingham, Alabama 35294, USA
    Mol Cell Proteomics 5:523-32. 2006
    ..Two secretory proteins, named as calcium-binding protein S100A9 and thymosin beta4, were identified by this novel technique. These two proteins thus may provide new insight into the enhancing effect of surfactants on skin vaccination...
  55. ncbi request reprint Proteomics and mass spectrometry in nutrition research
    Helen Kim
    Department of Pharmacology and Toxicology, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    Nutrition 20:155-65. 2004
  56. ncbi request reprint Murine mammary carcinoma exosomes promote tumor growth by suppression of NK cell function
    Cunren Liu
    Division of Clinical Immunology and Rheumatology, Department of Medicine, University of Alabama at Birmingham, AL 35294, USA
    J Immunol 176:1375-85. 2006
    ..We propose that tumor exosomes contribute to the growth of tumors by blocking IL-2-mediated activation of NK cells and their cytotoxic response to tumor cells...
  57. ncbi request reprint Mass spectrometric methods for the determination of flavonoids in biological samples
    Jeevan K Prasain
    Department of Pharmacology and Toxicology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    Free Radic Biol Med 37:1324-50. 2004
    ..Emphasis is placed on the description and value of existing methods, followed by an examination of emerging technologies...
  58. pmc The electrophile responsive proteome: integrating proteomics and lipidomics with cellular function
    Ashlee N Higdon
    Department of Pathology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    Antioxid Redox Signal 17:1580-9. 2012
    ..Through advanced analytical techniques, lipidomics is now emerging as a critical factor in our understanding of the pathology of a broad range of diseases...
  59. pmc Purification of CFTR for mass spectrometry analysis: identification of palmitoylation and other post-translational modifications
    Michelle McClure
    Gregory Fleming James Cystic Fibrosis Research Center, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    Protein Eng Des Sel 25:7-14. 2012
    ....
  60. ncbi request reprint Rat liver bile acid CoA:amino acid N-acyltransferase: expression, characterization, and peroxisomal localization
    Dongning He
    Department of Pharmacology and Toxicology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    J Lipid Res 44:2242-9. 2003
    ..In summary, rBAT is capable of forming both taurine and glycine bile acid conjugates and the enzyme is localized primarily in peroxisomes in rat liver...
  61. pmc The effect of mannan oligosaccharide supplementation on body weight gain and fat accrual in C57Bl/6J mice
    Daniel L Smith
    Department of Nutrition Sciences, University of Alabama at Birmingham, Birmingham, AL, USA
    Obesity (Silver Spring) 18:995-9. 2010
    ..247 g; P = 0.57). Contrary to previous research, MOS supplementation had no discernable effect on body weight gain or composition during this 12-week study, challenging the potential use of MOS as a CRM or body composition enhancer...
  62. pmc Effect of cranberry juice concentrate on chemically-induced urinary bladder cancers
    Jeevan K Prasain
    Department of Pharmacology and Toxicology, University of Alabama, Birmingham, AL 35294 0019, USA
    Oncol Rep 19:1565-70. 2008
    ..However, no quercetin was detected in the serum samples, indicating its poor bioavailability. These data suggest that components of cranberries may be effective in preventing urinary bladder carcinogenesis...
  63. pmc A novel nanoparticle drug delivery system: the anti-inflammatory activity of curcumin is enhanced when encapsulated in exosomes
    Dongmei Sun
    Department of Cell Biology, University of Alabama at Birmingham, Birmingham, Alabama, USA
    Mol Ther 18:1606-14. 2010
    ..The specificity of using exosomes as a drug carrier creates opportunities for treatments of many inflammation-related diseases without significant side effects due to innocent bystander or off-target effects...
  64. ncbi request reprint Revealing anti-inflammatory mechanisms of soy isoflavones by flow: modulation of leukocyte-endothelial cell interactions
    Balu K Chacko
    Department of Pathology, Univ of Alabama at Birmingham, 901 19th St South, BMR 2, Rm 307, Birmingham, AL 35294, USA
    Am J Physiol Heart Circ Physiol 289:H908-15. 2005
    ..These data reveal a novel anti-inflammatory mechanism for isoflavones and identify the physical forces associated with blood flow and a critical mediator of this function...
  65. ncbi request reprint Dietary phytoestrogens increase metabolic resistance (cold tolerance) in long-chain acyl-CoA dehydrogenase-deficient mice
    A Michele Schuler
    Department of Genetics, University of Alabama at Birmingham, USA
    J Nutr 134:1028-31. 2004
    ..Furthermore, they suggest changes that could improve pediatric formula constituents, especially with regard to management of children with inborn errors of fatty acid oxidation...
  66. ncbi request reprint Metabolism and bioavailability of flavonoids in chemoprevention: current analytical strategies and future prospectus
    Jeevan K Prasain
    Department of Pharmacology and Toxicology, Purdue UAB Botanicals Center for Age Related Disease, UAB Center for Nutrient Gene Interaction in Cancer Prevention, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    Mol Pharm 4:846-64. 2007
    ..This review describes the chemistry of polyphenols and flavonoids, their ADME, and the various mass spectrometry-based strategies used in the analysis of flavonoids, including future trends in this field...
  67. pmc Simultaneous determination of 11 phytoestrogens in human serum using a 2 min liquid chromatography/tandem mass spectrometry method
    Jeevan K Prasain
    Departments of Pharmacology and Toxicology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    J Chromatogr B Analyt Technol Biomed Life Sci 878:994-1002. 2010
    ..75 mL/min. This LC-MS/MS method is very useful for high-throughput analysis of phytoestrogens and proved to be simple, sensitive, reproducible, and reliable...
  68. pmc Role of phytoestrogens in cancer therapy
    Mandeep K Virk-Baker
    Department of Nutrition Sciences, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    Planta Med 76:1132-42. 2010
    ..We have reviewed the recent research studies in these areas in an attempt to find evidence for their role in cancer therapy as well as safety...
  69. ncbi request reprint Profiling and quantification of isoflavonoids in kudzu dietary supplements by high-performance liquid chromatography and electrospray ionization tandem mass spectrometry
    Jeevan K Prasain
    Department of Pharmacology and Toxicology, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    J Agric Food Chem 51:4213-8. 2003
    ..Puerarin, rather than daidzin, was the most abundant component (8.44-30.60 mg/capsule) in commercially available KDS...
  70. pmc A liquid chromatography mass spectrometry assay for determination of PD168393, a specific and irreversible inhibitor of erbB membrane tyrosine kinases, in rat serum
    Jeevan K Prasain
    Department of Pharmacology and Toxicology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    J Chromatogr B Analyt Technol Biomed Life Sci 876:219-24. 2008
    ..The validated method can be used in most or all stages of the screening and optimizing process for future method validation of pharmacokinetic studies...
  71. ncbi request reprint Differential biliary excretion of genistein metabolites following intraduodenal and intravenous infusion of genistin in female rats
    Jeevan K Prasain
    Department of Pharmacology and Toxicology, University of Alabama at Birmingham Botanicals Center for Age Related Disease, Birmingham, AL 35294, USA
    J Nutr 136:2975-9. 2006
    ..There was no evidence for any biliary secretion of the unreacted genistin, thereby excluding its uptake in the intact form from the small intestine in this animal model...
  72. ncbi request reprint Prospective highlights of functional skin proteomics
    Chun Ming Huang
    Department of Dermatology, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    Mass Spectrom Rev 24:647-60. 2005
    ..This type of research into functional skin proteomics will provide a critical step toward understanding disease and developing successful therapeutic strategies...
  73. ncbi request reprint Electrospray tandem mass spectrometric analysis of zeaxanthin and its oxidation products
    Jeevan Kumar Prasain
    Department of Pharmacology and Toxicology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    J Mass Spectrom 40:916-23. 2005
    ..Through the application of the LC/MS/MS method, we identified two oxime derivatives of 3-hydroxy-beta-ionone and 3-hydroxy-14'-apocarotenal with protonated molecules at m/z 252 and m/z 370 respectively, in a human eye sample...
  74. pmc Hemodynamic characteristics of midazolam, propofol, and dexmedetomidine in healthy volunteers
    Michael A Frölich
    Department of Anesthesiology, University of Alabama, Birmingham, AL 35249 6810, USA
    J Clin Anesth 23:218-23. 2011
    ..To study the effect of intravenous (IV) sedation on blood pressure (BP), heart rate (HR), and respiratory rates (RR) to determine if IV sedatives differ with respect to their effect on BP, HR, and RR...
  75. ncbi request reprint High throughput two-dimensional blue-native electrophoresis: a tool for functional proteomics of mitochondria and signaling complexes
    Paul S Brookes
    Department of Pathology, University of Alabama at Birmingham, Biomedical Research Building 2, 901 19th Street South, Birmingham, AL 35294, USA
    Proteomics 2:969-77. 2002
    ..Applications of this functional mitochondrial proteomics method are discussed...
  76. pmc Glycosylation patterns of HIV-1 gp120 depend on the type of expressing cells and affect antibody recognition
    Milan Raska
    Department of Immunology, Palacky University in Olomouc, Olomouc 77100, Czech Republic
    J Biol Chem 285:20860-9. 2010
    ..These results indicate that gp120 glycans contribute to antibody reactivity and should be considered in HIV-1 vaccine design...
  77. pmc Effect of a daily supplement of soy protein on body composition and insulin secretion in postmenopausal women
    Cynthia K Sites
    Department of Obstetrics and Gynecology and Pharmacology, University of Alabama at Birmingham, Birmingham, Alabama 35249, USA
    Fertil Steril 88:1609-17. 2007
    ..To determine whether a supplement of soy protein improves body composition, body fat distribution, and glucose and insulin metabolism in postmenopausal women without diabetes compared with an isocaloric casein placebo...
  78. ncbi request reprint Identification of puerarin and its metabolites in rats by liquid chromatography-tandem mass spectrometry
    Jeevan K Prasain
    Department of Pharmacology and Toxicology, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    J Agric Food Chem 52:3708-12. 2004
    ..Study of these metabolites may provide further understanding of the health beneficial effects of puerarin in kudzu dietary supplements...
  79. ncbi request reprint Molecular cloning and expression of rat liver bile acid CoA ligase
    Charles N Falany
    Department of Pharmacology and Toxicology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    J Lipid Res 43:2062-71. 2002
    ....
  80. ncbi request reprint A design and statistical perspective on microarray gene expression studies in nutrition: the need for playful creativity and scientific hard-mindedness
    Grier P Page
    Department of Biostatistics, Section on Statistical Genetics, University of Alabama at Birmingham, Birmingham, Alabama 35294 0022, USA
    Nutrition 19:997-1000. 2003
    ....
  81. pmc Serine protease PrtA from Streptococcus pneumoniae plays a role in the killing of S. pneumoniae by apolactoferrin
    Shaper Mirza
    Department of Microbiology, Division of Epidemiology, University of Alabama at Birmingham, 845 19th Street South, Birmingham, AL 35294 2170, USA
    Infect Immun 79:2440-50. 2011
    ....
  82. pmc Cleavage of p53-vimentin complex enhances tumor necrosis factor-related apoptosis-inducing ligand-mediated apoptosis of rheumatoid arthritis synovial fibroblasts
    Xinwen Yang
    Department of Medicine, University of Alabama at Birmingham, and the Birmingham Veterans Administration Medical Center, Birmingham, AL 35294 0007, USA
    Am J Pathol 167:705-19. 2005
    ..Taken together, our findings suggest a unique role for caspase-4 in cleaving vimentin and releasing cytosolic p53 for nuclear translocation, events that may regulate the sensitivity of RASFs to receptor-mediated apoptosis...
  83. ncbi request reprint Conserved residues in the putative catalytic triad of human bile acid Coenzyme A:amino acid N-acyltransferase
    Mindan K Sfakianos
    Department of Pharmacology and Toxicology, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    J Biol Chem 277:47270-5. 2002
    ....
  84. ncbi request reprint Effect of soy protein on endogenous hormones in postmenopausal women
    Victoria W Persky
    Division of Epidemiology and Biostatistics, The School of Public Health, The University of Illinois at Chicago, USA
    Am J Clin Nutr 75:145-53. 2002
    ..The long-term clinical effects of soy protein containing various concentrations of isoflavones on endogenous hormones are unknown...
  85. pmc Proteomic analysis of mice hippocampus in simulated microgravity environment
    Poonam Sarkar
    Molecular Neurotoxicology Laboratory and Proteomics Core, Department of Biology, Texas Southern University, Houston, Texas 77004, USA
    J Proteome Res 5:548-53. 2006
    ..This preliminary investigation leads to an understanding of the alteration of proteins in the hippocampus in response to the microgravity environment...
  86. pmc Assessment of dietary isoflavone intake among middle-aged Chinese men
    Sang Ah Lee
    Department of Medicine, Vanderbilt Epidemiology Center, Vanderbilt University and Vanderbilt Ingram Cancer Center, Nashville, TN 37232 8300, USA
    J Nutr 137:1011-6. 2007
    ..These results suggest that the SMHS FFQ can reliably and accurately measure usual intake of isoflavones, and that the levels of isoflavones in urine samples are relatively stable among men in Shanghai...
  87. ncbi request reprint Gaining insight into the health effects of soy but a long way still to go: commentary on the fourth International Symposium on the Role of Soy in Preventing and Treating Chronic Disease
    Mark Messina
    Nutrition Matters, Inc, Port Townsend, WA 98368, USA
    J Nutr 132:547S-551S. 2002
    ..If this modest amount of soy protein were to be incorporated in the American diet, it would represent only approximately 15% of total U. S. protein intake...
  88. pmc Botanicals for age-related diseases: from field to practice
    Connie M Weaver
    Foods and Nutrition, Rutgers University, New Brunswick, NJ, USA
    Am J Clin Nutr 87:493S-7S. 2008
    ..Botanicals and their relation to bone antiresorptive capacity, cognitive function, vascular effects, and cancer are principal themes in our center...
  89. ncbi request reprint Reliability and validity of an assessment of usual phytoestrogen consumption (United States)
    Pamela L Horn-Ross
    Northern California Cancer Center, 2201 Walnut Ave, Suite 300, Fremont, CA 94538, USA
    Cancer Causes Control 17:85-93. 2006
    ..To evaluate the reliability and validity of a food-frequency questionnaire (FFQ) and database designed to quantify phytoestrogen consumption...
  90. pmc Evaluating statistical methods using plasmode data sets in the age of massive public databases: an illustration using false discovery rates
    Gary L Gadbury
    Department of Statistics, Kansas State University, Manhattan, Kansas, United States of America
    PLoS Genet 4:e1000098. 2008
    ..We use the procedure to compare estimates for the proportion of true null hypotheses, the false discovery rate (FDR), and a local version of FDR obtained from 15 different statistical methods...

Research Grants5

  1. 9.4 Tesla Fourier Transform Mass Spectrometer
    Stephen Barnes; Fiscal Year: 2002
    ..Overall, the FT-MS instrument will sustain the high quality of biomedical research that is a hallmark of UAB. ..
  2. A sensitive triple quadrupole mass spectrometer
    Stephen Barnes; Fiscal Year: 2004
    ..It is anticipated that a commercial nanospray interface for use in nanoLC-MS analysis will be available by the fall of 2003. ..
  3. AMINO ACID CONJUGATION OF BILE ACIDS
    Stephen Barnes; Fiscal Year: 2006
    ..abstract_text> ..
  4. Center for Nutrient-Gene Interaction in Cancer Prevention
    Stephen Barnes; Fiscal Year: 2007
    ..In addition to the main projects, the Center will organize a competitive Pilot Project/Developmental program for the evaluation of new ideas and methods pertinent to the overall development of the Center. ..