Georjana Barnes

Summary

Affiliation: University of California
Country: USA

Publications

  1. ncbi request reprint Phospho-regulation of kinetochore-microtubule attachments by the Aurora kinase Ipl1p
    Iain M Cheeseman
    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
    Cell 111:163-72. 2002
  2. pmc Mitotic spindle integrity and kinetochore function linked by the Duo1p/Dam1p complex
    I M Cheeseman
    Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720, USA
    J Cell Biol 152:197-212. 2001
  3. pmc Architecture of the budding yeast kinetochore reveals a conserved molecular core
    Stefan Westermann
    Department of Molecular and Cell Biology, Barker Hall, University of California, Berkeley, Berkeley, CA 94720, USA
    J Cell Biol 163:215-22. 2003
  4. pmc Overlapping kinetochore targets of CK2 and Aurora B kinases in mitotic regulation
    Yutian Peng
    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
    Mol Biol Cell 22:2680-9. 2011
  5. pmc Nbl1p: a Borealin/Dasra/CSC-1-like protein essential for Aurora/Ipl1 complex function and integrity in Saccharomyces cerevisiae
    Yuko Nakajima
    Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, CA 94720, USA
    Mol Biol Cell 20:1772-84. 2009
  6. pmc A protein interaction map of the mitotic spindle
    Jonathan Wong
    Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA
    Mol Biol Cell 18:3800-9. 2007
  7. pmc Mitotic spindle disassembly occurs via distinct subprocesses driven by the anaphase-promoting complex, Aurora B kinase, and kinesin-8
    Jeffrey B Woodruff
    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
    J Cell Biol 191:795-808. 2010
  8. ncbi request reprint Architecture of the Dam1 kinetochore ring complex and implications for microtubule-driven assembly and force-coupling mechanisms
    Hong Wei Wang
    Life Sciences Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Rd, Berkeley, California 94720, USA
    Nat Struct Mol Biol 14:721-6. 2007
  9. ncbi request reprint The Dam1 kinetochore ring complex moves processively on depolymerizing microtubule ends
    Stefan Westermann
    Department of Molecular and Cell Biology, University of California Berkeley, Berkeley, California 94720 3202, USA
    Nature 440:565-9. 2006
  10. ncbi request reprint Formation of a dynamic kinetochore- microtubule interface through assembly of the Dam1 ring complex
    Stefan Westermann
    Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, CA 94720, USA
    Mol Cell 17:277-90. 2005

Research Grants

  1. Saccharomyces cerevisiae microtuble cytoskeleton
    Georjana Barnes; Fiscal Year: 2009
  2. Saccharomyces cerevisiae microtuble cytoskeleton
    Georjana Barnes; Fiscal Year: 2009
  3. SACCHAROMYCES CEREVISIAE MICROTUBULE CYTOSKELETON
    Georjana Barnes; Fiscal Year: 2005
  4. SACCHAROMYCES CEREVISIAE MICROTUBULE CYTOSKELETON
    Georjana Barnes; Fiscal Year: 2001
  5. SACCHAROMYCES CEREVISIAE MICROTUBULE CYTOSKELETON
    Georjana Barnes; Fiscal Year: 1993
  6. SACCHAROMYCES CEREVISIAE MICROTUBULE CYTOSKELETON
    Georjana Barnes; Fiscal Year: 2010

Collaborators

Detail Information

Publications19

  1. ncbi request reprint Phospho-regulation of kinetochore-microtubule attachments by the Aurora kinase Ipl1p
    Iain M Cheeseman
    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
    Cell 111:163-72. 2002
    ..Our systematic mutational analysis of the Ipl1p phosphorylation sites demonstrated that the essential microtubule binding protein Dam1p is a key Ipl1p target for regulating kinetochore-microtubule attachments in vivo...
  2. pmc Mitotic spindle integrity and kinetochore function linked by the Duo1p/Dam1p complex
    I M Cheeseman
    Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720, USA
    J Cell Biol 152:197-212. 2001
    ..In addition, Duo1p and Dam1p localize to kinetochores in chromosome spreads, suggesting that this complex may serve as a link between the kinetochore and the mitotic spindle...
  3. pmc Architecture of the budding yeast kinetochore reveals a conserved molecular core
    Stefan Westermann
    Department of Molecular and Cell Biology, Barker Hall, University of California, Berkeley, Berkeley, CA 94720, USA
    J Cell Biol 163:215-22. 2003
    ..We propose that a molecular core consisting of CENP-A, -C, -H, and Ndc80/HEC has been conserved from yeast to humans to link centromeres to spindle microtubules...
  4. pmc Overlapping kinetochore targets of CK2 and Aurora B kinases in mitotic regulation
    Yutian Peng
    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
    Mol Biol Cell 22:2680-9. 2011
    ..Furthermore, we show Mif2 and the inner kinetochore protein Ndc10 are phosphorylated by CK2, and this phosphorylation plays antagonistic and synergistic roles with Aurora B phosphorylation of these targets, respectively...
  5. pmc Nbl1p: a Borealin/Dasra/CSC-1-like protein essential for Aurora/Ipl1 complex function and integrity in Saccharomyces cerevisiae
    Yuko Nakajima
    Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, CA 94720, USA
    Mol Biol Cell 20:1772-84. 2009
    ..Our biological and computational analyses therefore establish that the fundamental design of the CPC is conserved from Fungi to Animalia...
  6. pmc A protein interaction map of the mitotic spindle
    Jonathan Wong
    Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA
    Mol Biol Cell 18:3800-9. 2007
    ..The wealth of protein interactions presented here highlights the extent to which mitotic spindle protein functions and regulation are integrated with each other and with other cellular activities...
  7. pmc Mitotic spindle disassembly occurs via distinct subprocesses driven by the anaphase-promoting complex, Aurora B kinase, and kinesin-8
    Jeffrey B Woodruff
    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
    J Cell Biol 191:795-808. 2010
    ..Combinatorial inhibition of these subprocesses yielded cells with hyperstable spindle remnants and dramatic defects in cell cycle progression, establishing that rapid spindle disassembly is crucial for cell proliferation...
  8. ncbi request reprint Architecture of the Dam1 kinetochore ring complex and implications for microtubule-driven assembly and force-coupling mechanisms
    Hong Wei Wang
    Life Sciences Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Rd, Berkeley, California 94720, USA
    Nat Struct Mol Biol 14:721-6. 2007
    ..One of Ipl1's roles might be to fine-tune the coupling of the microtubule interaction with the conformational change required for oligomerization, with phosphorylation resulting in ring breakdown...
  9. ncbi request reprint The Dam1 kinetochore ring complex moves processively on depolymerizing microtubule ends
    Stefan Westermann
    Department of Molecular and Cell Biology, University of California Berkeley, Berkeley, California 94720 3202, USA
    Nature 440:565-9. 2006
    ..The Dam1 ring complex is a molecular device that can translate the force generated by microtubule depolymerization into movement along the lattice to facilitate chromosome segregation...
  10. ncbi request reprint Formation of a dynamic kinetochore- microtubule interface through assembly of the Dam1 ring complex
    Stefan Westermann
    Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, CA 94720, USA
    Mol Cell 17:277-90. 2005
    ..These observations suggest a mechanism by which the kinetochore can recognize and stay attached to the plus ends of microtubules...
  11. pmc Architecture and flexibility of the yeast Ndc80 kinetochore complex
    Hong Wei Wang
    Life Science Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, CA 94720, USA
    J Mol Biol 383:894-903. 2008
    ..We hypothesize that the kink in Ndc80 is essential for correct kinetochore geometry and could be part of a tension-sensing mechanism at the kinetochore...
  12. ncbi request reprint Structures and functions of yeast kinetochore complexes
    Stefan Westermann
    Department of Molecular and Cell Biology, University of California, Berkeley, California 94720, USA
    Annu Rev Biochem 76:563-91. 2007
    ..Here we review the current understanding of kinetochore function in budding yeast and draw comparisons to recent findings in other organisms...
  13. pmc Subunit organization in the Dam1 kinetochore complex and its ring around microtubules
    Vincent H Ramey
    Biophysics Graduate Group, University of California, Berkeley, Berkeley, CA 94720, USA
    Mol Biol Cell 22:4335-42. 2011
    ..Integrating this information with previously published data, we generated a structural model for the Dam1 complex assembly that advances our understanding of its function and will direct future experiments...
  14. pmc Kinetochore protein interactions and their regulation by the Aurora kinase Ipl1p
    Ching Shang
    Department of Molecular and Cell Biology, University of California, Berkeley, California 94720 3202, USA
    Mol Biol Cell 14:3342-55. 2003
    ..Strikingly, we found that the Dam1p-Ndc80p and Dam1p-Spc34p interactions were weakened by mutations mimicking phosphorylation at Ipl1p sites, allowing us to formulate a model for the effects of phosphoregulation on kinetochore function...
  15. pmc The Dam1 ring binds to the E-hook of tubulin and diffuses along the microtubule
    Vincent H Ramey
    Biophysics Graduate Group, University of California, Berkeley, Berkeley, CA 94720, USA
    Mol Biol Cell 22:457-66. 2011
    ..The present studies constitute a significant step forward by linking structural and biochemical observations toward a comprehensive understanding of the Dam1 complex...
  16. pmc Ipl1/Aurora-dependent phosphorylation of Sli15/INCENP regulates CPC-spindle interaction to ensure proper microtubule dynamics
    Yuko Nakajima
    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
    J Cell Biol 194:137-53. 2011
    ....
  17. pmc Spindle assembly requires complete disassembly of spindle remnants from the previous cell cycle
    Jeffrey B Woodruff
    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
    Mol Biol Cell 23:258-67. 2012
    ..Therefore we propose that spindle disassembly is essential for regeneration of the intracellular pool of assembly-competent tubulin required for efficient spindle assembly during subsequent mitoses of daughter cells...
  18. pmc Dynein-driven mitotic spindle positioning restricted to anaphase by She1p inhibition of dynactin recruitment
    Jeffrey B Woodruff
    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
    Mol Biol Cell 20:3003-11. 2009
    ..We propose that She1p restricts dynein-dependent spindle positioning to anaphase by inhibiting the association of dynein with the complete dynactin complex...
  19. pmc Simple centromere, complex kinetochore: linking spindle microtubules and centromeric DNA in budding yeast
    Iain M Cheeseman
    Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720 3202, USA
    J Cell Biol 157:199-203. 2002
    ..This analysis may provide general principles for kinetochore function and regulation...

Research Grants17

  1. Saccharomyces cerevisiae microtuble cytoskeleton
    Georjana Barnes; Fiscal Year: 2009
    ..Chromosome instability is a key contributing factor in cancer and birth defects. Therefore, understanding principles of spindle function may suggest novel strategies for prevention, detection and treatment of human diseases. ..
  2. Saccharomyces cerevisiae microtuble cytoskeleton
    Georjana Barnes; Fiscal Year: 2009
    ..Chromosome instability is a key contributing factor in cancer and birth defects. Therefore, understanding principles of spindle function may suggest novel strategies for prevention, detection and treatment of human diseases. ..
  3. SACCHAROMYCES CEREVISIAE MICROTUBULE CYTOSKELETON
    Georjana Barnes; Fiscal Year: 2005
    ..Comparison of how these mutations affect mitosis and meiosis II vs. meiosis I promises to provide a deeper understanding of the unique properties of meiosis I. ..
  4. SACCHAROMYCES CEREVISIAE MICROTUBULE CYTOSKELETON
    Georjana Barnes; Fiscal Year: 2001
    ..In total, these studies will define components of the microtubule and actin cytoskeletons that are required to ensure proper chromosome inheritance. ..
  5. SACCHAROMYCES CEREVISIAE MICROTUBULE CYTOSKELETON
    Georjana Barnes; Fiscal Year: 1993
    ..In total, these studies will provide novel insights into the mechanisms by which accessory proteins interact with tubulin in living cells to mediate such functions as chromosome segregation, nuclear migration and nuclear fusion...
  6. SACCHAROMYCES CEREVISIAE MICROTUBULE CYTOSKELETON
    Georjana Barnes; Fiscal Year: 2010
    ..Chromosome instability is a key contributing factor in cancer and birth defects. Therefore, understanding principles of spindle function may suggest novel strategies for prevention, detection and treatment of human diseases. ..