Research Topics
| RUMA V BANERJEESummaryAffiliation: University of Nebraska Country: USA Publications
Research Grants
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Detail Information
Publications
The many faces of vitamin B12: catalysis by cobalamin-dependent enzymesRuma Banerjee
Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
Annu Rev Biochem 72:209-47. 2003....- Radical carbon skeleton rearrangements: catalysis by coenzyme B12-dependent mutasesRuma Banerjee
Biochemistry Department, University of Nebraska, Lincoln, Nebraska 68588-0664, USA
Chem Rev 103:2083-94. 2003 - Reaction mechanism and regulation of cystathionine beta-synthaseRuma Banerjee
Biochemistry Department, University of Nebraska, Lincoln, NE 68588 0664, USA
Biochim Biophys Acta 1647:30-5. 2003.... - Controlling the reactivity of radical intermediates by coenzyme B(12)-dependent methylmalonyl-CoA mutaseR Banerjee
Biochemistry Department, University of Nebraska, Lincoln, NE 68588 0664, USA
Biochem Soc Trans 30:621-4. 2002..In this review, insights into this issue that have emerged from kinetic, mutagenesis and structural studies are described for methylmalonyl-CoA mutase... - B12 trafficking in mammals: A for coenzyme escort serviceRuma Banerjee
Redox Biology Center and the Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
ACS Chem Biol 1:149-59. 2006.... 
Quantum catalysis in B12-dependent methylmalonyl-CoA mutase: experimental and computational insightsRuma Banerjee
Biochemistry Department, University of Nebraska, Lincoln, NE 68588 0664, USA
Philos Trans R Soc Lond B Biol Sci 361:1333-9. 2006....- Visualization of PLP-bound intermediates in hemeless variants of human cystathionine beta-synthase: evidence that lysine 119 is a general baseRuby Evande
Biochemistry Department, University of Nebraska, Lincoln, NE 68588-0664, USA
Arch Biochem Biophys 427:188-96. 2004..These results are consistent with an additional role for K119 as a general base in the reaction catalyzed by human cystathionine beta-synthase... - Alleviation of intrasteric inhibition by the pathogenic activation domain mutation, D444N, in human cystathionine beta-synthaseRuby Evande
Biochemistry Department, University of Nebraska, Lincoln, NE 68588 0664, USA
Biochemistry 41:11832-7. 2002..e., wild-type enzyme as isolated), "activated" (wild-type enzyme + AdoMet or the D444N mutant as isolated), and superactivated (D444N mutant + AdoMet or wild-type enzyme lacking the C-terminal regulatory domain)... - Human cystathionine beta-synthase is a heme sensor protein. Evidence that the redox sensor is heme and not the vicinal cysteines in the CXXC motif seen in the crystal structure of the truncated enzymeShinichi Taoka
Biochemistry Department, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
Biochemistry 41:10454-61. 2002.... - Mirror "base-off" conformation of coenzyme B12 in human adenosyltransferase and its downstream target, methylmalonyl-CoA mutaseMamoru Yamanishi
Biochemistry Department, University of Nebraska, Lincoln, Nebraska 68588-0664, USA
J Am Chem Soc 127:526-7. 2005..However, the coordination environment for cobalt in the two proteins is distinct, which is reflected in an approximately 40-fold difference in their affinity for the cofactor... - When a spectator turns killer: suicidal electron transfer from cobalamin in methylmalonyl-CoA mutaseMonica D Vlasie
Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588-0664, USA
Biochemistry 43:8410-7. 2004..These studies provide evidence for the critical role of active site residues in controlling radical reactivity and thereby suppressing inactivating side reactions... - Stopped-flow kinetic analysis of the reaction catalyzed by the full-length yeast cystathionine beta-synthaseShinichi Taoka
Biochemistry Department, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
J Biol Chem 277:22421-5. 2002..H., Niks, D., McPhie, P., Dunn, M. F., and Miles, E. W. (2001) Biochemistry 40, 10873-10880)... - Effects of heme ligand mutations including a pathogenic variant, H65R, on the properties of human cystathionine beta-synthaseSunil Ojha
Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588-0664, USA
Biochemistry 41:4649-54. 2002..Both H65 and C52 variants display low catalytic activity, revealing that changes in the heme binding domain modulate activity, consistent with a regulatory role for this cofactor... - Alternative pathways for radical dissipation in an active site mutant of B12-dependent methylmalonyl-CoA mutaseDominique Padovani
Redox Biology Center and Biochemistry Department, University of Nebraska, Lincoln, Nebraska 68588-0664, USA
Biochemistry 45:2951-9. 2006..These studies serve to emphasize the fine control exerted by Y243 in the vicinity of the substrate to minimize radical extinction in side reactions... - Tyrosine 89 accelerates Co-carbon bond homolysis in methylmalonyl-CoA mutaseMonica D Vlasie
Biochemistry Department, University of Nebraska, Lincoln, Nebraska 68588-0664, USA
J Am Chem Soc 125:5431-5. 2003..Together, these results are consistent with homolysis becoming completely rate determining in the forward direction in the two mutants and points to the role of Y89 as a molecular wedge in accelerating Co-carbon bond cleavage... - Importance of the histidine ligand to coenzyme B12 in the reaction catalyzed by methylmalonyl-CoA mutaseMonica Vlasie
Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588-0664, USA
J Biol Chem 277:18523-7. 2002.... - Kinetic and thermodynamic characterization of the common polymorphic variants of human methionine synthase reductaseHoratiu Olteanu
Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
Biochemistry 43:1988-97. 2004..It is likely that differences in their relative affinities for the redox partner, methionine synthase, underlie the differences in the relative efficiencies of reductive activation exhibited by the variants... - Mapping peptides correlated with transmission of intrasteric inhibition and allosteric activation in human cystathionine beta-synthaseSuvajit Sen
Biochemistry Department, University of Nebraska, Lincoln, Nebraska 68588-0664, USA
Biochemistry 44:14210-6. 2005..Our hydrogen exchange data identify surfaces that are potentially involved in the juxtaposition of the regulatory and catalytic domains and form the basis of a docked structural model for the full-length enzyme... - Properties of an unusual heme cofactor in PLP-dependent cystathionine beta-synthaseSangita Singh
Redox Biology Center and Department of Biochemistry, University of Nebraska, Lincoln, NE 68588-0664, USA
Nat Prod Rep 24:631-9. 2007 - Redox regulation and reaction mechanism of human cystathionine-beta-synthase: a PLP-dependent hemesensor proteinRuma Banerjee
Department of Biochemistry, University of Nebraska, Lincoln, NE 68588 0664, USA
Arch Biochem Biophys 433:144-56. 2005.... - Structural insights into pathogenic mutations in heme-dependent cystathionine-beta-synthaseMamoru Yamanishi
Redox Biology Center and Department of Biological Chemistry, University of Nebraska, Lincoln, NE 68588-0664, USA
J Inorg Biochem 100:1988-95. 2006.... - Adenosyltransferase: an enzyme and an escort for coenzyme B12?Mamoru Yamanishi
Biochemistry Department, University of Nebraska, Lincoln, NE 68588 0664, USA
Trends Biochem Sci 30:304-8. 2005.... - A pathogenic linked mutation in the catalytic core of human cystathionine beta-synthase disrupts allosteric regulation and allows kinetic characterization of a full-length dimerSuvajit Sen
Redox Biology Center and the Biochemistry Department, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
Biochemistry 46:4110-6. 2007..Furthermore, analysis of individual single mutations has permitted, for the first time, partial kinetic characterization of a full-length dimeric form of human cystathionine beta-synthase... - Impeded electron transfer from a pathogenic FMN domain mutant of methionine synthase reductase and its responsiveness to flavin supplementationCarmen G Gherasim
Biochemistry Department, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
Biochemistry 47:12515-22. 2008.... - Testosterone regulation of homocysteine metabolism modulates redox status in human prostate cancer cellsAnna Prudova
Redox Biology Center and the Biochemistry Department, University of Nebraska, Lincoln, Nebraska, USA
Antioxid Redox Signal 9:1875-81. 2007..These results demonstrate regulation of the homocysteine-clearing enzyme, CBS, by testosterone and suggest the potential utility of targeting this enzyme as a chemotherapeutic strategy... - Kinetic properties of polymorphic variants and pathogenic mutants in human cystathionine gamma-lyaseWeidong Zhu
Redox Biology Center and Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
Biochemistry 47:6226-32. 2008..These results reveal that both mutations weaken the affinity for PLP and suggest that cystathionuric patients with these mutations should be responsive to pyridoxine therapy... - Energetics of interaction between the G-protein chaperone, MeaB, and B12-dependent methylmalonyl-CoA mutaseDominique Padovani
Redox Biology Center and the Biochemistry Department, University of Nebraska, Lincoln, NE 68588 0664, USA
J Biol Chem 281:17838-44. 2006..These studies provide insights into the energetics of interaction between the radical enzyme methylmalonyl-CoA mutase and MeaB, which are discussed... - S-adenosylmethionine stabilizes cystathionine beta-synthase and modulates redox capacityAnna Prudova
Redox Biology Center and Biochemistry Department, University of Nebraska, Lincoln, NE 68588 0664, USA
Proc Natl Acad Sci U S A 103:6489-94. 2006..A mechanistic basis for the coordinate changes in redox and methylation metabolism that are a hallmark of several complex diseases is explained by these observations... - Assembly and protection of the radical enzyme, methylmalonyl-CoA mutase, by its chaperoneDominique Padovani
Redox Biology Center and Biochemistry Department, University of Nebraska, Lincoln, Nebraska 68588-0664, USA
Biochemistry 45:9300-6. 2006..This study suggests that MeaB functions in the GTP-dependent assembly of holomethylmalonyl-CoA mutase and subsequent protection of radical intermediates during catalysis... - A B12-responsive internal ribosome entry site (IRES) element in human methionine synthaseSebastian Oltean
Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
J Biol Chem 280:32662-8. 2005..Modulation of the IRES-dependent translation of an essential gene by the cofactor of the encoded enzyme represents a novel example of a gene-nutrient interaction... - Homocysteine and redox signalingCheng-Gang Zou
Biochemistry Department, University of Nebraska, Lincoln, NE 68588, USA
Antioxid Redox Signal 7:547-59. 2005..We also discuss redox regulation of the key enzymes involved in homocysteine clearance: methionine synthase, betaine-homocysteine methyltranferase, and cystathionine beta-synthase... - Redundancy in the pathway for redox regulation of mammalian methionine synthase: reductive activation by the dual flavoprotein, novel reductase 1Horatiu Olteanu
Biochemistry Department, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
J Biol Chem 278:38310-4. 2003.... - Tumor necrosis factor-alpha-induced targeted proteolysis of cystathionine beta-synthase modulates redox homeostasisCheng-Gang Zou
Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, Nebraska 68588-0664, USA
J Biol Chem 278:16802-8. 2003..Together, these data reveal a novel and previously unknown mechanism of regulation for homocysteine-linked glutathione homeostasis in cells challenged by oxidative stress... - Human cystathionine beta-synthase is a target for sumoylationOmer Kabil
Redox Biology Center, Biochemistry Department, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
Biochemistry 45:13528-36. 2006..The discovery that CBS is a target of sumoylation adds another layer to the complex regulation of this enzyme and reveals a previously unknown residence for this protein, i.e., in the nucleus... - Differences in the efficiency of reductive activation of methionine synthase and exogenous electron acceptors between the common polymorphic variants of human methionine synthase reductaseHoratiu Olteanu
Biochemistry Department, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
Biochemistry 41:13378-85. 2002..These results reveal differences in the interactions between the natural and artificial electron acceptors and MSR variants in vitro, which are predicted to result in less efficient reductive repair of methionine synthase in vivo... - Testosterone regulation of renal cystathionine beta-synthase: implications for sex-dependent differences in plasma homocysteine levelsVictor Vitvitsky
Redox Biology Center and the Biochemistry Dept, University of Nebraska, Lincoln, NE 68588 0664, USA
Am J Physiol Renal Physiol 293:F594-600. 2007..Our data suggest that testosterone-dependent regulation of human CBS in kidney may contribute to sex-dependent differences in homocysteine transsulfuration... - Perturbations in homocysteine-linked redox homeostasis in a murine model for hyperhomocysteinemiaVictor Vitvitsky
Biochemistry Dept, Univ of Nebraska, Lincoln, NE 68588 0664, USA
Am J Physiol Regul Integr Comp Physiol 287:R39-46. 2004.... - Polymorphic background of methionine synthase reductase modulates the phenotype of a disease-causing mutationCarmen Gherasim
Redox Biology Center and Biochemistry Department, University of Nebraska Lincoln, Lincoln, Nebraska 68588 0664, USA
Hum Mutat 28:1028-33. 2007..Val56Met mutation and the p.Ile22Met variation, which was confirmed by sequence analysis. This study reveals how a genetic variation can modulate phenotypic expression of a disease-causing mutation... - Nutritional modulation of gene expression and homocysteine utilization by vitamin B12Sebastian Oltean
Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
J Biol Chem 278:20778-84. 2003.... - Expression profiling of homocysteine junction enzymes in the NCI60 panel of human cancer cell linesWen Zhang
Department of Biochemistry, University of Nebraska, Lincoln, Nebraska, USA
Cancer Res 65:1554-60. 2005.... - Analysis of pathological defects in methionine metabolism using a simple mathematical modelAnna Prudova
Department of Biochemistry, University of Nebraska, Lincoln, NE 68588-0664, USA
Biochim Biophys Acta 1741:331-8. 2005..The theoretical predictions were found to be in good agreement with experimental data obtained with the human hepatoma cell line, HepG2... - Monocyte differentiation, activation, and mycobacterial killing are linked to transsulfuration-dependent redox metabolismSanjay Garg
Redox Biology Center and the Department of Biochemistry, University of Nebraska, Lincoln, NE 68588-0664, USA
J Biol Chem 281:38712-20. 2006....
Research Grants
- A Radical Enzyme and its EscortsRuma Banerjee; Fiscal Year: 2007....
- GENE-NUTRIENT INTERACTIONS IN REDOX HOMEOSTASISRuma Banerjee; Fiscal Year: 2002....
- Gordon Research Sponsored Conference on CobalaminsRuma Banerjee; Fiscal Year: 2003....
- Transsulfuration and HyperhomocysteinemiaRuma Banerjee; Fiscal Year: 2007....
- FASEB Summer Conference: Folic Acid, Vitamin B12 and One Carbon MetabolismRuma Banerjee; Fiscal Year: 2007..The poster sessions highlight the activities of the younger participants and the number of short oral presentations chosen from abstracts submitted by junior attendees has been increased. ..
- Regulation of Homocysteine-dependent Redox HomeostasisRuma Banerjee; Fiscal Year: 2007..abstract_text> ..