RUMA V BANERJEE

Summary

Affiliation: University of Nebraska
Country: USA

Publications

  1. ncbi request reprint The many faces of vitamin B12: catalysis by cobalamin-dependent enzymes
    Ruma Banerjee
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
    Annu Rev Biochem 72:209-47. 2003
  2. ncbi request reprint Radical carbon skeleton rearrangements: catalysis by coenzyme B12-dependent mutases
    Ruma Banerjee
    Biochemistry Department, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
    Chem Rev 103:2083-94. 2003
  3. ncbi request reprint Reaction mechanism and regulation of cystathionine beta-synthase
    Ruma Banerjee
    Biochemistry Department, University of Nebraska, Lincoln, NE 68588 0664, USA
    Biochim Biophys Acta 1647:30-5. 2003
  4. ncbi request reprint Controlling the reactivity of radical intermediates by coenzyme B(12)-dependent methylmalonyl-CoA mutase
    R Banerjee
    Biochemistry Department, University of Nebraska, Lincoln, NE 68588 0664, USA
    Biochem Soc Trans 30:621-4. 2002
  5. ncbi request reprint B12 trafficking in mammals: A for coenzyme escort service
    Ruma Banerjee
    Redox Biology Center and the Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
    ACS Chem Biol 1:149-59. 2006
  6. pmc Quantum catalysis in B12-dependent methylmalonyl-CoA mutase: experimental and computational insights
    Ruma Banerjee
    Biochemistry Department, University of Nebraska, Lincoln, NE 68588 0664, USA
    Philos Trans R Soc Lond B Biol Sci 361:1333-9. 2006
  7. ncbi request reprint Visualization of PLP-bound intermediates in hemeless variants of human cystathionine beta-synthase: evidence that lysine 119 is a general base
    Ruby Evande
    Biochemistry Department, University of Nebraska, Lincoln, NE 68588 0664, USA
    Arch Biochem Biophys 427:188-96. 2004
  8. ncbi request reprint Alleviation of intrasteric inhibition by the pathogenic activation domain mutation, D444N, in human cystathionine beta-synthase
    Ruby Evande
    Biochemistry Department, University of Nebraska, Lincoln, NE 68588 0664, USA
    Biochemistry 41:11832-7. 2002
  9. ncbi request reprint Human cystathionine beta-synthase is a heme sensor protein. Evidence that the redox sensor is heme and not the vicinal cysteines in the CXXC motif seen in the crystal structure of the truncated enzyme
    Shinichi Taoka
    Biochemistry Department, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
    Biochemistry 41:10454-61. 2002
  10. ncbi request reprint Mirror "base-off" conformation of coenzyme B12 in human adenosyltransferase and its downstream target, methylmalonyl-CoA mutase
    Mamoru Yamanishi
    Biochemistry Department, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
    J Am Chem Soc 127:526-7. 2005

Research Grants

Collaborators

  • STEVEN LENTZ
  • S P Stabler
  • Stephen W Ragsdale
  • Nigel S Scrutton
  • Jianmin Wu
  • Kirsten R Wolthers
  • Andrew W Munro
  • SHELLY CHI LOO LU
  • Hong Wang
  • Anna Prudova
  • Victor Vitvitsky
  • Mamoru Yamanishi
  • Dominique Padovani
  • Horatiu Olteanu
  • Suvajit Sen
  • Omer Kabil
  • Sebastian Oltean
  • Cheng Gang Zou
  • Monica D Vlasie
  • Ruby Evande
  • Sunil Ojha
  • Zachary Bauman
  • Shinichi Taoka
  • Monica Vlasie
  • Weidong Zhu
  • Carmen G Gherasim
  • Alexander Lin
  • Carmen Gherasim
  • Sangita Singh
  • Peter Madzelan
  • Sanjay Garg
  • Tetyana Labunska
  • Aaron Braun
  • Sanjana Dayal
  • You Zhou
  • Wen Zhang
  • Ashraf Raza
  • Uzma Zaman
  • Matthias Albin
  • David S Rosenblatt
  • Howard E Gendelman
  • Jiong Yu
  • Daniel Schellhorn
  • Victor M Vitvitsky
  • Mikhail V Martinov
  • Fazoil I Ataullakhanov
  • Shantanu Chowdhury
  • Bryan W Lepore
  • Henk Blom
  • Troy Munson
  • Dagmar Ringe
  • Godfried H J Boers
  • Russell Lobrutto

Detail Information

Publications42

  1. ncbi request reprint The many faces of vitamin B12: catalysis by cobalamin-dependent enzymes
    Ruma Banerjee
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
    Annu Rev Biochem 72:209-47. 2003
    ....
  2. ncbi request reprint Radical carbon skeleton rearrangements: catalysis by coenzyme B12-dependent mutases
    Ruma Banerjee
    Biochemistry Department, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
    Chem Rev 103:2083-94. 2003
  3. ncbi request reprint Reaction mechanism and regulation of cystathionine beta-synthase
    Ruma Banerjee
    Biochemistry Department, University of Nebraska, Lincoln, NE 68588 0664, USA
    Biochim Biophys Acta 1647:30-5. 2003
    ....
  4. ncbi request reprint Controlling the reactivity of radical intermediates by coenzyme B(12)-dependent methylmalonyl-CoA mutase
    R Banerjee
    Biochemistry Department, University of Nebraska, Lincoln, NE 68588 0664, USA
    Biochem Soc Trans 30:621-4. 2002
    ..In this review, insights into this issue that have emerged from kinetic, mutagenesis and structural studies are described for methylmalonyl-CoA mutase...
  5. ncbi request reprint B12 trafficking in mammals: A for coenzyme escort service
    Ruma Banerjee
    Redox Biology Center and the Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
    ACS Chem Biol 1:149-59. 2006
    ....
  6. pmc Quantum catalysis in B12-dependent methylmalonyl-CoA mutase: experimental and computational insights
    Ruma Banerjee
    Biochemistry Department, University of Nebraska, Lincoln, NE 68588 0664, USA
    Philos Trans R Soc Lond B Biol Sci 361:1333-9. 2006
    ....
  7. ncbi request reprint Visualization of PLP-bound intermediates in hemeless variants of human cystathionine beta-synthase: evidence that lysine 119 is a general base
    Ruby Evande
    Biochemistry Department, University of Nebraska, Lincoln, NE 68588 0664, USA
    Arch Biochem Biophys 427:188-96. 2004
    ..These results are consistent with an additional role for K119 as a general base in the reaction catalyzed by human cystathionine beta-synthase...
  8. ncbi request reprint Alleviation of intrasteric inhibition by the pathogenic activation domain mutation, D444N, in human cystathionine beta-synthase
    Ruby Evande
    Biochemistry Department, University of Nebraska, Lincoln, NE 68588 0664, USA
    Biochemistry 41:11832-7. 2002
    ..e., wild-type enzyme as isolated), "activated" (wild-type enzyme + AdoMet or the D444N mutant as isolated), and superactivated (D444N mutant + AdoMet or wild-type enzyme lacking the C-terminal regulatory domain)...
  9. ncbi request reprint Human cystathionine beta-synthase is a heme sensor protein. Evidence that the redox sensor is heme and not the vicinal cysteines in the CXXC motif seen in the crystal structure of the truncated enzyme
    Shinichi Taoka
    Biochemistry Department, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
    Biochemistry 41:10454-61. 2002
    ....
  10. ncbi request reprint Mirror "base-off" conformation of coenzyme B12 in human adenosyltransferase and its downstream target, methylmalonyl-CoA mutase
    Mamoru Yamanishi
    Biochemistry Department, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
    J Am Chem Soc 127:526-7. 2005
    ..However, the coordination environment for cobalt in the two proteins is distinct, which is reflected in an approximately 40-fold difference in their affinity for the cofactor...
  11. ncbi request reprint When a spectator turns killer: suicidal electron transfer from cobalamin in methylmalonyl-CoA mutase
    Monica D Vlasie
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
    Biochemistry 43:8410-7. 2004
    ..These studies provide evidence for the critical role of active site residues in controlling radical reactivity and thereby suppressing inactivating side reactions...
  12. ncbi request reprint Stopped-flow kinetic analysis of the reaction catalyzed by the full-length yeast cystathionine beta-synthase
    Shinichi Taoka
    Biochemistry Department, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
    J Biol Chem 277:22421-5. 2002
    ..H., Niks, D., McPhie, P., Dunn, M. F., and Miles, E. W. (2001) Biochemistry 40, 10873-10880)...
  13. ncbi request reprint Effects of heme ligand mutations including a pathogenic variant, H65R, on the properties of human cystathionine beta-synthase
    Sunil Ojha
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
    Biochemistry 41:4649-54. 2002
    ..Both H65 and C52 variants display low catalytic activity, revealing that changes in the heme binding domain modulate activity, consistent with a regulatory role for this cofactor...
  14. pmc Alternative pathways for radical dissipation in an active site mutant of B12-dependent methylmalonyl-CoA mutase
    Dominique Padovani
    Redox Biology Center and Biochemistry Department, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
    Biochemistry 45:2951-9. 2006
    ..These studies serve to emphasize the fine control exerted by Y243 in the vicinity of the substrate to minimize radical extinction in side reactions...
  15. ncbi request reprint Tyrosine 89 accelerates Co-carbon bond homolysis in methylmalonyl-CoA mutase
    Monica D Vlasie
    Biochemistry Department, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
    J Am Chem Soc 125:5431-5. 2003
    ..Together, these results are consistent with homolysis becoming completely rate determining in the forward direction in the two mutants and points to the role of Y89 as a molecular wedge in accelerating Co-carbon bond cleavage...
  16. ncbi request reprint Importance of the histidine ligand to coenzyme B12 in the reaction catalyzed by methylmalonyl-CoA mutase
    Monica Vlasie
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
    J Biol Chem 277:18523-7. 2002
    ....
  17. ncbi request reprint Kinetic and thermodynamic characterization of the common polymorphic variants of human methionine synthase reductase
    Horatiu Olteanu
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
    Biochemistry 43:1988-97. 2004
    ..It is likely that differences in their relative affinities for the redox partner, methionine synthase, underlie the differences in the relative efficiencies of reductive activation exhibited by the variants...
  18. ncbi request reprint Mapping peptides correlated with transmission of intrasteric inhibition and allosteric activation in human cystathionine beta-synthase
    Suvajit Sen
    Biochemistry Department, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
    Biochemistry 44:14210-6. 2005
    ..Our hydrogen exchange data identify surfaces that are potentially involved in the juxtaposition of the regulatory and catalytic domains and form the basis of a docked structural model for the full-length enzyme...
  19. ncbi request reprint Properties of an unusual heme cofactor in PLP-dependent cystathionine beta-synthase
    Sangita Singh
    Redox Biology Center and Department of Biochemistry, University of Nebraska, Lincoln, NE 68588 0664, USA
    Nat Prod Rep 24:631-9. 2007
  20. ncbi request reprint Redox regulation and reaction mechanism of human cystathionine-beta-synthase: a PLP-dependent hemesensor protein
    Ruma Banerjee
    Department of Biochemistry, University of Nebraska, Lincoln, NE 68588 0664, USA
    Arch Biochem Biophys 433:144-56. 2005
    ....
  21. ncbi request reprint Structural insights into pathogenic mutations in heme-dependent cystathionine-beta-synthase
    Mamoru Yamanishi
    Redox Biology Center and Department of Biological Chemistry, University of Nebraska, Lincoln, NE 68588 0664, USA
    J Inorg Biochem 100:1988-95. 2006
    ....
  22. ncbi request reprint Adenosyltransferase: an enzyme and an escort for coenzyme B12?
    Mamoru Yamanishi
    Biochemistry Department, University of Nebraska, Lincoln, NE 68588 0664, USA
    Trends Biochem Sci 30:304-8. 2005
    ....
  23. pmc A pathogenic linked mutation in the catalytic core of human cystathionine beta-synthase disrupts allosteric regulation and allows kinetic characterization of a full-length dimer
    Suvajit Sen
    Redox Biology Center and the Biochemistry Department, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
    Biochemistry 46:4110-6. 2007
    ..Furthermore, analysis of individual single mutations has permitted, for the first time, partial kinetic characterization of a full-length dimeric form of human cystathionine beta-synthase...
  24. pmc Impeded electron transfer from a pathogenic FMN domain mutant of methionine synthase reductase and its responsiveness to flavin supplementation
    Carmen G Gherasim
    Biochemistry Department, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
    Biochemistry 47:12515-22. 2008
    ....
  25. ncbi request reprint Testosterone regulation of homocysteine metabolism modulates redox status in human prostate cancer cells
    Anna Prudova
    Redox Biology Center and the Biochemistry Department, University of Nebraska, Lincoln, Nebraska, USA
    Antioxid Redox Signal 9:1875-81. 2007
    ..These results demonstrate regulation of the homocysteine-clearing enzyme, CBS, by testosterone and suggest the potential utility of targeting this enzyme as a chemotherapeutic strategy...
  26. pmc Kinetic properties of polymorphic variants and pathogenic mutants in human cystathionine gamma-lyase
    Weidong Zhu
    Redox Biology Center and Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
    Biochemistry 47:6226-32. 2008
    ..These results reveal that both mutations weaken the affinity for PLP and suggest that cystathionuric patients with these mutations should be responsive to pyridoxine therapy...
  27. ncbi request reprint Energetics of interaction between the G-protein chaperone, MeaB, and B12-dependent methylmalonyl-CoA mutase
    Dominique Padovani
    Redox Biology Center and the Biochemistry Department, University of Nebraska, Lincoln, NE 68588 0664, USA
    J Biol Chem 281:17838-44. 2006
    ..These studies provide insights into the energetics of interaction between the radical enzyme methylmalonyl-CoA mutase and MeaB, which are discussed...
  28. pmc S-adenosylmethionine stabilizes cystathionine beta-synthase and modulates redox capacity
    Anna Prudova
    Redox Biology Center and Biochemistry Department, University of Nebraska, Lincoln, NE 68588 0664, USA
    Proc Natl Acad Sci U S A 103:6489-94. 2006
    ..A mechanistic basis for the coordinate changes in redox and methylation metabolism that are a hallmark of several complex diseases is explained by these observations...
  29. ncbi request reprint Assembly and protection of the radical enzyme, methylmalonyl-CoA mutase, by its chaperone
    Dominique Padovani
    Redox Biology Center and Biochemistry Department, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
    Biochemistry 45:9300-6. 2006
    ..This study suggests that MeaB functions in the GTP-dependent assembly of holomethylmalonyl-CoA mutase and subsequent protection of radical intermediates during catalysis...
  30. ncbi request reprint A B12-responsive internal ribosome entry site (IRES) element in human methionine synthase
    Sebastian Oltean
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
    J Biol Chem 280:32662-8. 2005
    ..Modulation of the IRES-dependent translation of an essential gene by the cofactor of the encoded enzyme represents a novel example of a gene-nutrient interaction...
  31. ncbi request reprint Homocysteine and redox signaling
    Cheng Gang Zou
    Biochemistry Department, University of Nebraska, Lincoln, NE 68588, USA
    Antioxid Redox Signal 7:547-59. 2005
    ..We also discuss redox regulation of the key enzymes involved in homocysteine clearance: methionine synthase, betaine-homocysteine methyltranferase, and cystathionine beta-synthase...
  32. ncbi request reprint Redundancy in the pathway for redox regulation of mammalian methionine synthase: reductive activation by the dual flavoprotein, novel reductase 1
    Horatiu Olteanu
    Biochemistry Department, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
    J Biol Chem 278:38310-4. 2003
    ....
  33. ncbi request reprint Tumor necrosis factor-alpha-induced targeted proteolysis of cystathionine beta-synthase modulates redox homeostasis
    Cheng Gang Zou
    Department of Biochemistry, University of Nebraska Lincoln, Lincoln, Nebraska 68588 0664, USA
    J Biol Chem 278:16802-8. 2003
    ..Together, these data reveal a novel and previously unknown mechanism of regulation for homocysteine-linked glutathione homeostasis in cells challenged by oxidative stress...
  34. ncbi request reprint Human cystathionine beta-synthase is a target for sumoylation
    Omer Kabil
    Redox Biology Center, Biochemistry Department, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
    Biochemistry 45:13528-36. 2006
    ..The discovery that CBS is a target of sumoylation adds another layer to the complex regulation of this enzyme and reveals a previously unknown residence for this protein, i.e., in the nucleus...
  35. ncbi request reprint Differences in the efficiency of reductive activation of methionine synthase and exogenous electron acceptors between the common polymorphic variants of human methionine synthase reductase
    Horatiu Olteanu
    Biochemistry Department, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
    Biochemistry 41:13378-85. 2002
    ..These results reveal differences in the interactions between the natural and artificial electron acceptors and MSR variants in vitro, which are predicted to result in less efficient reductive repair of methionine synthase in vivo...
  36. ncbi request reprint Testosterone regulation of renal cystathionine beta-synthase: implications for sex-dependent differences in plasma homocysteine levels
    Victor Vitvitsky
    Redox Biology Center and the Biochemistry Dept, University of Nebraska, Lincoln, NE 68588 0664, USA
    Am J Physiol Renal Physiol 293:F594-600. 2007
    ..Our data suggest that testosterone-dependent regulation of human CBS in kidney may contribute to sex-dependent differences in homocysteine transsulfuration...
  37. ncbi request reprint Perturbations in homocysteine-linked redox homeostasis in a murine model for hyperhomocysteinemia
    Victor Vitvitsky
    Biochemistry Dept, Univ of Nebraska, Lincoln, NE 68588 0664, USA
    Am J Physiol Regul Integr Comp Physiol 287:R39-46. 2004
    ....
  38. ncbi request reprint Polymorphic background of methionine synthase reductase modulates the phenotype of a disease-causing mutation
    Carmen Gherasim
    Redox Biology Center and Biochemistry Department, University of Nebraska Lincoln, Lincoln, Nebraska 68588 0664, USA
    Hum Mutat 28:1028-33. 2007
    ..Val56Met mutation and the p.Ile22Met variation, which was confirmed by sequence analysis. This study reveals how a genetic variation can modulate phenotypic expression of a disease-causing mutation...
  39. ncbi request reprint Nutritional modulation of gene expression and homocysteine utilization by vitamin B12
    Sebastian Oltean
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
    J Biol Chem 278:20778-84. 2003
    ....
  40. ncbi request reprint Expression profiling of homocysteine junction enzymes in the NCI60 panel of human cancer cell lines
    Wen Zhang
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska, USA
    Cancer Res 65:1554-60. 2005
    ....
  41. ncbi request reprint Analysis of pathological defects in methionine metabolism using a simple mathematical model
    Anna Prudova
    Department of Biochemistry, University of Nebraska, Lincoln, NE 68588 0664, USA
    Biochim Biophys Acta 1741:331-8. 2005
    ..The theoretical predictions were found to be in good agreement with experimental data obtained with the human hepatoma cell line, HepG2...
  42. ncbi request reprint Monocyte differentiation, activation, and mycobacterial killing are linked to transsulfuration-dependent redox metabolism
    Sanjay Garg
    Redox Biology Center and the Department of Biochemistry, University of Nebraska, Lincoln, NE 68588 0664, USA
    J Biol Chem 281:38712-20. 2006
    ....

Research Grants6

  1. A Radical Enzyme and its Escorts
    Ruma Banerjee; Fiscal Year: 2007
    ....
  2. GENE-NUTRIENT INTERACTIONS IN REDOX HOMEOSTASIS
    Ruma Banerjee; Fiscal Year: 2002
    ....
  3. Gordon Research Sponsored Conference on Cobalamins
    Ruma Banerjee; Fiscal Year: 2003
    ....
  4. Transsulfuration and Hyperhomocysteinemia
    Ruma Banerjee; Fiscal Year: 2007
    ....
  5. FASEB Summer Conference: Folic Acid, Vitamin B12 and One Carbon Metabolism
    Ruma Banerjee; Fiscal Year: 2007
    ..The poster sessions highlight the activities of the younger participants and the number of short oral presentations chosen from abstracts submitted by junior attendees has been increased. ..
  6. Regulation of Homocysteine-dependent Redox Homeostasis
    Ruma Banerjee; Fiscal Year: 2007
    ..abstract_text> ..