Lata Balakrishnan

Summary

Affiliation: University of Rochester
Country: USA

Publications

  1. pmc Flap endonuclease 1
    Lata Balakrishnan
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
    Annu Rev Biochem 82:119-38. 2013
  2. pmc Biochemical analyses indicate that binding and cleavage specificities define the ordered processing of human Okazaki fragments by Dna2 and FEN1
    Jason W Gloor
    Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA
    Nucleic Acids Res 40:6774-86. 2012
  3. pmc Components of the secondary pathway stimulate the primary pathway of eukaryotic Okazaki fragment processing
    Ryan A Henry
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
    J Biol Chem 285:28496-505. 2010
  4. pmc Telomere proteins POT1, TRF1 and TRF2 augment long-patch base excision repair in vitro
    Adam S Miller
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, NY, USA
    Cell Cycle 11:998-1007. 2012
  5. pmc Msh2-Msh3 interferes with Okazaki fragment processing to promote trinucleotide repeat expansions
    Athena Kantartzis
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, NY 14642, USA
    Cell Rep 2:216-22. 2012
  6. pmc Dna2 exhibits a unique strand end-dependent helicase function
    Lata Balakrishnan
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
    J Biol Chem 285:38861-8. 2010
  7. pmc Flap endonuclease 1 mechanism analysis indicates flap base binding prior to threading
    Jason W Gloor
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
    J Biol Chem 285:34922-31. 2010
  8. pmc Reconstitution of eukaryotic lagging strand DNA replication
    Lata Balakrishnan
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA
    Methods 51:347-57. 2010
  9. pmc Acetylation of Dna2 endonuclease/helicase and flap endonuclease 1 by p300 promotes DNA stability by creating long flap intermediates
    Lata Balakrishnan
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
    J Biol Chem 285:4398-404. 2010
  10. pmc Eukaryotic lagging strand DNA replication employs a multi-pathway mechanism that protects genome integrity
    Lata Balakrishnan
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
    J Biol Chem 286:6865-70. 2011

Collaborators

  • Patricia L Opresko
  • Barry Milavetz
  • Robert A Bambara
  • Jason W Gloor
  • Athena Kantartzis
  • Adam S Miller
  • Judith L Campbell
  • Ryan A Henry
  • Noah A Buncher
  • Rick L Roberts
  • Jennifer A Surtees
  • Gregory M Williams
  • Stefanie Tan Ying-Lin

Detail Information

Publications12

  1. pmc Flap endonuclease 1
    Lata Balakrishnan
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
    Annu Rev Biochem 82:119-38. 2013
    ..Overall, recent advances in our knowledge of FEN1 suggest that it was an ancient protein that has been fine-tuned over eons to coordinate many essential DNA transactions...
  2. pmc Biochemical analyses indicate that binding and cleavage specificities define the ordered processing of human Okazaki fragments by Dna2 and FEN1
    Jason W Gloor
    Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA
    Nucleic Acids Res 40:6774-86. 2012
    ..The DNA can then equilibrate to a double flap that can bind Dna2 and flap endonuclease (FEN1) simultaneously. When Dna2 shortens the flap even more, FEN1 can displace the Dna2 and cleave at the flap base to make a nick for ligation...
  3. pmc Components of the secondary pathway stimulate the primary pathway of eukaryotic Okazaki fragment processing
    Ryan A Henry
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
    J Biol Chem 285:28496-505. 2010
    ..These findings also support a model in which cleavage by FEN1 alone is the preferred pathway, with the first opportunity to complete cleavage, and is stimulated by components of the backup pathway...
  4. pmc Telomere proteins POT1, TRF1 and TRF2 augment long-patch base excision repair in vitro
    Adam S Miller
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, NY, USA
    Cell Cycle 11:998-1007. 2012
    ..Thought to protect telomere DNA from degradation, these proteins still apparently evolved to allow selective access of repair proteins...
  5. pmc Msh2-Msh3 interferes with Okazaki fragment processing to promote trinucleotide repeat expansions
    Athena Kantartzis
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, NY 14642, USA
    Cell Rep 2:216-22. 2012
    ..We believe that this is the first mechanistic evidence showing the interplay of replication and repair proteins in the expansion of sequences during lagging-strand DNA replication...
  6. pmc Dna2 exhibits a unique strand end-dependent helicase function
    Lata Balakrishnan
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
    J Biol Chem 285:38861-8. 2010
    ..Biologically, the loading requirement likely helps the helicase to coordinate with the Dna2 nuclease function to prevent creation of undesirably long flaps during DNA transactions...
  7. pmc Flap endonuclease 1 mechanism analysis indicates flap base binding prior to threading
    Jason W Gloor
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
    J Biol Chem 285:34922-31. 2010
    ..Apparently, interactions related to threading occur only when the flap is greater than two to four nucleotides long, implying that short flaps are cleaved without a threading requirement...
  8. pmc Reconstitution of eukaryotic lagging strand DNA replication
    Lata Balakrishnan
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA
    Methods 51:347-57. 2010
    ..In this review, we describe both the design of specific DNA substrates that simulate intermediates of fragment maturation and protocols for reconstituting partial and complete lagging strand replication...
  9. pmc Acetylation of Dna2 endonuclease/helicase and flap endonuclease 1 by p300 promotes DNA stability by creating long flap intermediates
    Lata Balakrishnan
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
    J Biol Chem 285:4398-404. 2010
    ..For example, altering the ratio between short and long flap Okazaki fragment processing would be a mechanism for better correction of the error-prone synthesis catalyzed by DNA polymerase alpha...
  10. pmc Eukaryotic lagging strand DNA replication employs a multi-pathway mechanism that protects genome integrity
    Lata Balakrishnan
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
    J Biol Chem 286:6865-70. 2011
    ..There is now evidence that post-translational protein modification regulates the flow through the pathways to favor protection of genomic information in regions of actively transcribed chromatin...
  11. pmc Long patch base excision repair proceeds via coordinated stimulation of the multienzyme DNA repair complex
    Lata Balakrishnan
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
    J Biol Chem 284:15158-72. 2009
    ..Our results imply that this mechanism is still carried out, but in the context of a multienzyme complex that remains structurally intact during the repair process...
  12. doi request reprint Decoding the histone H4 lysine 20 methylation mark
    Lata Balakrishnan
    Department of Biochemistry and Biophysics, University of Rochester, Rochester, NY, USA
    Crit Rev Biochem Mol Biol 45:440-52. 2010
    ....