I S Ayene
Affiliation: University of Pennsylvania
- DNA as an important target in radiation-induced apoptosis of MYC and MYC plus RAS transfected rat embryo fibroblastsI S Ayene
University of Pennsylvania School of Medicine, Department of Radiation Oncology, Philadelphia 19104 6072, USA
Int J Radiat Biol 76:343-54. 2000..This study uses a radiation chemistry approach to determine if DNA is an important target for radiation-induced apoptosis of myc (MR4) and myc plus ras (3.7) transfected rat embryo fibroblast cell lines...
- Oxidation of cellular thiols by hydroxyethyldisulphide inhibits DNA double-strand-break rejoining in G6PD deficient mammalian cellsI S Ayene
Department of Radiation Oncology, University of Pennsylvania School of Medicine, Philadelphia 19104, USA
Int J Radiat Biol 76:1523-31. 2000..We investigated the effect of protein- and non protein-thiol oxidation on DNA double-strand-break (DSB) rejoining after irradiation and its relevance in the survival of CHO cells...
- Mutation in the glucose-6-phosphate dehydrogenase gene leads to inactivation of Ku DNA end binding during oxidative stressIraimoudi S Ayene
Department of Radiation Oncology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
J Biol Chem 277:9929-35. 2002..In view of the rapidly escalating number of roles ascribed to Ku, these results may have widespread ramifications...
- Ku protein targeting by Ku70 small interfering RNA enhances human cancer cell response to topoisomerase II inhibitor and gamma radiationIraimoudi S Ayene
Department of Radiation Oncology, School of Medicine, University of Pennsylvania, 195 John Morgan Building, Philadelphia, PA 19104 6072, USA
Mol Cancer Ther 4:529-36. 2005..Studies with HCT116 cells using the same Ku70 siRNA (CS #5) showed a direct correlation between expression of Ku70 and sensitization to radiation and etoposide treatments...
- Radiation response of cells during altered protein thiol redoxJohn E Biaglow
Department of Radiation Oncology and Biochemistry, University of Pennsylvania Medical School, Philadelphia, Pennsylvania 19104, USA
Radiat Res 159:484-94. 2003..The results also show that protein thiol status is a critical factor in cell survival after irradiation...
- Role of vicinal protein thiols in radiation and cytotoxic responsesJohn E Biaglow
Department of Biochemistry, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
Radiat Res 165:307-17. 2006..We conclude that radiation sensitivity and cell survival are dependent on vicinal thiol and GSH. In the former and latter cases, the protein thiols are also important...
- DNA strand breakage by bivalent metal ions and ionizing radiationIraimoudi S Ayene
Department of Radiation Oncology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 4283, USA
Int J Radiat Biol 83:195-210. 2007..To investigate mechanisms of DNA breakage via the interaction of bivalent metal ion, thiol reducing agent and ionizing radiation, in *OH scavenging abilities comparable to those in cells...
- Mutation in G6PD gene leads to loss of cellular control of protein glutathionylation: mechanism and implicationIraimoudi S Ayene
Lankenau Institute for Medical Research, 100 Lancaster Avenue, Wynnewood, PA 19096, USA
J Cell Biochem 103:123-35. 2008..The specific role of G6PD in the control of such global protein glutathionylation and Ku function was further demonstrated by reintroducing the G6PD gene into E89 (A1A) cells, which showed a normal phenotype...
- Detection of reactive oxygen species via endogenous oxidative pentose phosphate cycle activity in response to oxygen concentration: implications for the mechanism of HIF-1alpha stabilization under moderate hypoxiaStephen W Tuttle
Department of Radiation Oncology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 6072, USA
J Biol Chem 282:36790-6. 2007..Therefore, we conclude that the oxygen dependence of the prolyl hydroxylase reaction is sufficient to mediate HIF-1alpha stability under moderate as well as severe hypoxia...