Daniel Axelrod

Summary

Affiliation: University of Michigan
Country: USA

Publications

  1. ncbi request reprint Direct measurement of the evanescent field profile produced by objective-based total internal reflection fluorescence
    Alexa L Mattheyses
    University of Michigan, Biophysics Research Division, Ann Arbor, Michigan 48109, USA
    J Biomed Opt 11:014006. 2006
  2. pmc Evanescent excitation and emission in fluorescence microscopy
    Daniel Axelrod
    Department of Physics, Department of Biophysics, and Department of Pharmacology, University of Michigan, Ann Arbor, Michigan Electronic address
    Biophys J 104:1401-9. 2013
  3. doi request reprint Fluorescence excitation and imaging of single molecules near dielectric-coated and bare surfaces: a theoretical study
    Daniel Axelrod
    Department of Physics, University of Michigan, Ann Arbor, Michigan 48109, USA
    J Microsc 247:147-60. 2012
  4. pmc Restriction of secretory granule motion near the plasma membrane of chromaffin cells
    L M Johns
    Department of Pharmacology, The University of Michigan, Ann Arbor, Michigan 48109, USA
    J Cell Biol 153:177-90. 2001
  5. ncbi request reprint Selective imaging of surface fluorescence with very high aperture microscope objectives
    D Axelrod
    University of Michigan, Department of Physics and Biophysics Research Division, Ann Arbor 48109, USA
    J Biomed Opt 6:6-13. 2001
  6. ncbi request reprint Total internal reflection fluorescence microscopy in cell biology
    D Axelrod
    Department of Physics and Biophysics Research Division, University of Michigan, Ann Arbor, MI 48109, USA
    Traffic 2:764-74. 2001
  7. ncbi request reprint Combinatorial microscopy
    Daniel Axelrod
    Department of Physics and Biophysics Research Division, University of Michigan, Ann Arbor, Michigan 48109, USA
    Nat Rev Mol Cell Biol 7:944-52. 2006
  8. pmc The structural and functional implications of linked SNARE motifs in SNAP25
    Li Wang
    Department of Pharmacology, University of Michigan, Ann Arbor, MI 48109 5632, USA
    Mol Biol Cell 19:3944-55. 2008
  9. pmc A new role for the dynamin GTPase in the regulation of fusion pore expansion
    Arun Anantharam
    Department of Pharmacology, University of Michigan, Ann Arbor, MI 48109, USA
    Mol Biol Cell 22:1907-18. 2011
  10. pmc Localized topological changes of the plasma membrane upon exocytosis visualized by polarized TIRFM
    Arun Anantharam
    Department of Pharmacology, University of Michigan, Ann Arbor, MI 48109, USA
    J Cell Biol 188:415-28. 2010

Detail Information

Publications27

  1. ncbi request reprint Direct measurement of the evanescent field profile produced by objective-based total internal reflection fluorescence
    Alexa L Mattheyses
    University of Michigan, Biophysics Research Division, Ann Arbor, Michigan 48109, USA
    J Biomed Opt 11:014006. 2006
    ....
  2. pmc Evanescent excitation and emission in fluorescence microscopy
    Daniel Axelrod
    Department of Physics, Department of Biophysics, and Department of Pharmacology, University of Michigan, Ann Arbor, Michigan Electronic address
    Biophys J 104:1401-9. 2013
    ..Analogously, the phase properties of evanescent emission lead to a method of producing a smaller point spread function, in a technique called virtual supercritical angle fluorescence...
  3. doi request reprint Fluorescence excitation and imaging of single molecules near dielectric-coated and bare surfaces: a theoretical study
    Daniel Axelrod
    Department of Physics, University of Michigan, Ann Arbor, Michigan 48109, USA
    J Microsc 247:147-60. 2012
    ..This theoretical analysis discusses how these features can be used to report film thickness and refractive index, and fluorophore axial position and orientation...
  4. pmc Restriction of secretory granule motion near the plasma membrane of chromaffin cells
    L M Johns
    Department of Pharmacology, The University of Michigan, Ann Arbor, Michigan 48109, USA
    J Cell Biol 153:177-90. 2001
    ..However, the lack of functional SNAREs on the plasma or granule membranes in such cells reduces the time that some granules spend immediately adjacent to the PM...
  5. ncbi request reprint Selective imaging of surface fluorescence with very high aperture microscope objectives
    D Axelrod
    University of Michigan, Department of Physics and Biophysics Research Division, Ann Arbor 48109, USA
    J Biomed Opt 6:6-13. 2001
    ..Schematic diagrams, experimental demonstrations, and practical suggestions for all these techniques are provided...
  6. ncbi request reprint Total internal reflection fluorescence microscopy in cell biology
    D Axelrod
    Department of Physics and Biophysics Research Division, University of Michigan, Ann Arbor, MI 48109, USA
    Traffic 2:764-74. 2001
    ..A brief summary of these applications is provided, followed by presentations of the physical basis for the technique and the various ways to implement total internal reflection fluorescence in a standard fluorescence microscope...
  7. ncbi request reprint Combinatorial microscopy
    Daniel Axelrod
    Department of Physics and Biophysics Research Division, University of Michigan, Ann Arbor, Michigan 48109, USA
    Nat Rev Mol Cell Biol 7:944-52. 2006
    ..We are now within reach of viewing the motions, orientations, binding kinetics and specific transient associations of previously 'submicroscopic' cellular structures and single molecules...
  8. pmc The structural and functional implications of linked SNARE motifs in SNAP25
    Li Wang
    Department of Pharmacology, University of Michigan, Ann Arbor, MI 48109 5632, USA
    Mol Biol Cell 19:3944-55. 2008
    ..The experiments suggest that the bidentate structure permits specific conformations in complexes with syntaxin and VAMP and facilitates the function of SN1 and SN2 in exocytosis...
  9. pmc A new role for the dynamin GTPase in the regulation of fusion pore expansion
    Arun Anantharam
    Department of Pharmacology, University of Michigan, Ann Arbor, MI 48109, USA
    Mol Biol Cell 22:1907-18. 2011
    ..These findings expand the membrane-sculpting repertoire of dynamin to include the regulation of immediate postfusion events in exocytosis that control the rate of release of soluble granule contents...
  10. pmc Localized topological changes of the plasma membrane upon exocytosis visualized by polarized TIRFM
    Arun Anantharam
    Department of Pharmacology, University of Michigan, Ann Arbor, MI 48109, USA
    J Cell Biol 188:415-28. 2010
    ..We provide direct evidence for a persistent curvature in the exocytotic region that is altered by inhibition of dynamin guanosine triphosphatase activity and is temporally distinct from endocytosis measured by VMAT2-pHluorin...
  11. pmc Motion matters: secretory granule motion adjacent to the plasma membrane and exocytosis
    Miriam W Allersma
    Department of Pharmacology, University of Michigan, Ann Arbor, MI 48109 0632, USA
    Mol Biol Cell 17:2424-38. 2006
    ..Motion continues until shortly before fusion, suggesting that interaction of granule and plasma membrane proteins is transient. Disruption of actin dynamics did not significantly alter granule motion...
  12. pmc Visualization of regulated exocytosis with a granule-membrane probe using total internal reflection microscopy
    Miriam W Allersma
    Department of Pharmacology, University of Michigan, Ann Arbor, MI 48109, USA
    Mol Biol Cell 15:4658-68. 2004
    ..Overall granule behavior before and during fusion is strikingly similar to exocytosis previously described in the constitutive secretory pathway...
  13. pmc Dynamic light scattering microscopy. A novel optical technique to image submicroscopic motions. II: Experimental applications
    Rhonda Dzakpasu
    Department of Physics and Biophysics Research Division, University of Michigan, Ann Arbor, Michigan, USA
    Biophys J 87:1288-97. 2004
    ..The rates can be used to construct an image-like spatial map of the rapidity of submicroscopic motions of scattering centers...
  14. pmc Increased motion and travel, rather than stable docking, characterize the last moments before secretory granule fusion
    Vadim E Degtyar
    Department of Pharmacology, University of Michigan, Ann Arbor, MI 48104 0632, USA
    Proc Natl Acad Sci U S A 104:15929-34. 2007
    ..Thus, instead of being stably docked before exocytosis, granules undergo molecular-scale motions and travel immediately preceding the fusion event...
  15. ncbi request reprint Fluorescence emission patterns near glass and metal-coated surfaces investigated with back focal plane imaging
    Alexa L Mattheyses
    University of Michigan, Biophysics Research Division, Ann Arbor, Michigan 48109, USA
    J Biomed Opt 10:054007. 2005
    ..The observed profiles agree well with computer calculations and suggest some optical modifications that are potentially useful in cell biophysics...
  16. pmc Polarized TIRFM reveals changes in plasma membrane topology before and during granule fusion
    Arun Anantharam
    Department of Pharmacology, University of Michigan, 1150 W Medical Center Dr, 2315 MSRB III, Ann Arbor, MI 48109, USA
    Cell Mol Neurobiol 30:1343-9. 2010
    ..When Sr2+ is used instead of Ca2+ to trigger exocytosis, membrane topology in the exocytotic region is stabilized with significant curvature and indentation...
  17. doi request reprint Chapter 7: Total internal reflection fluorescence microscopy
    Daniel Axelrod
    Departments of Physics and Biophysics, University of Michigan, Ann Arbor, Michigan 48109, USA
    Methods Cell Biol 89:169-221. 2008
    ....
  18. ncbi request reprint Effective elimination of laser interference fringing in fluorescence microscopy by spinning azimuthal incidence angle
    Alexa L Mattheyses
    Biophysics Research Division, University of Michigan, Ann Arbor, Michigan 48109, USA
    Microsc Res Tech 69:642-7. 2006
    ..If the wedge is spun rapidly, then the different interference patterns at every particular azimuthal incidence angle average out over a single camera exposure to produce an effectively uniform field of illumination...
  19. pmc Polarized fluorescence resonance energy transfer microscopy
    Alexa L Mattheyses
    Biophysics Research Division, Department of Microbiology and Immunology, University of Michigan, Ann Arbor, Michigan 48109, USA
    Biophys J 87:2787-97. 2004
    ..The effects of shot noise, acceptor polarization, and FRET efficiency on the statistical accuracy of p-FRET experimental results are investigated by a noise-simulation program...
  20. pmc Membrane-proximal calcium transients in stimulated neutrophils detected by total internal reflection fluorescence
    G M Omann
    Department of Surgery, University of Michigan, Ann Arbor 48105, USA
    Biophys J 71:2885-91. 1996
    ..This method should be applicable to a wide variety of cell types and fluorescent ion indicators in which membrane-proximal ionic transients may be different from those deeper within the cytosol...
  21. ncbi request reprint Secretory granule behaviour adjacent to the plasma membrane before and during exocytosis: total internal reflection fluorescence microscopy studies
    R W Holz
    Department of Pharmacology, University of Michigan, Ann Arbor, MI 48109 5632, USA
    Acta Physiol (Oxf) 192:303-7. 2008
    ..Increased travel may increase the probability of granules interacting productively with the plasma membrane constituents, thereby, increasing the probability of fusion...
  22. ncbi request reprint G protein threshold behavior in the human neutrophil oxidant response: measurement of G proteins available for signaling in responding and nonresponding subpopulations
    Peter S Chang
    Department of Chemical Engineering, University of Michigan, Ann Arbor, MI 48109, USA
    Cell Signal 17:605-14. 2005
    ....
  23. pmc Real-time imaging of plasma membrane deformations reveals pre-fusion membrane curvature changes and a role for dynamin in the regulation of fusion pore expansion
    Arun Anantharam
    Department of Biological Sciences, Wayne State University, Detroit, MI 48202, USA
    J Neurochem 122:661-71. 2012
    ..Finally, we discuss how expansion of the fusion pore may be regulated by the GTPase activity of dynamin...
  24. pmc Dynamic light scattering microscopy. A novel optical technique to image submicroscopic motions. I: theory
    Rhonda Dzakpasu
    Department of Physics and Biophysics Research Division, University of Michigan, Ann Arbor, Michigan, USA
    Biophys J 87:1279-87. 2004
    ..The accompanying article in this issue describes an experimental implementation of dynamic light scattering microscopy...
  25. ncbi request reprint Localization of phosphatidylinositol 4,5-P(2) important in exocytosis and a quantitative analysis of chromaffin granule motion adjacent to the plasma membrane
    Ronald W Holz
    Department of Pharmacology and Department of Physics, Biophysics Research Division, University of Michigan, Ann Arbor, Michigan 48109, USA
    Ann N Y Acad Sci 971:232-43. 2002
    ..The quantitative analysis indicates that chromaffin granule motion is highly restricted and suggests that chromaffin granules are caged or tethered immediately adjacent to the plasma membrane...
  26. ncbi request reprint Total internal reflection fluorescence microscopy in cell biology
    Daniel Axelrod
    Department of Physics and Biophysics Research Division, University of Michigan, Ann Arbor, Michigan 48109, USA
    Methods Enzymol 361:1-33. 2003
  27. pmc Reversible binding kinetics of a cytoskeletal protein at the erythrocyte submembrane
    A L Stout
    Biophysics Research Division, University of Michigan, Ann Arbor, Michigan 48109 1055
    Biophys J 67:1324-34. 1994
    ....