Richard Auchus

Summary

Affiliation: University of Texas Southwestern Medical Center
Country: USA

Publications

  1. ncbi request reprint Aldo is back: recent advances and unresolved controversies in hyperaldosteronism
    Richard J Auchus
    Division of Endocrinology and Metabolism, UT Southwestern Medical Center, Dallas, Texas 75390, USA
    Curr Opin Nephrol Hypertens 12:153-8. 2003
  2. doi request reprint Congenital adrenal hyperplasia in adults
    Richard J Auchus
    Division of Endocrinology and Metabolism, Department of Internal Medicine, UT Southwestern Medical Center, Dallas, Texas 75390 8857, USA
    Curr Opin Endocrinol Diabetes Obes 17:210-6. 2010
  3. doi request reprint Primary aldosteronism and a Texas two-step
    Richard J Auchus
    Division of Endocrinology and Metabolism, UT Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX 75390 8857, USA
    Rev Endocr Metab Disord 12:37-42. 2011
  4. ncbi request reprint Overview of dehydroepiandrosterone biosynthesis
    Richard J Auchus
    Division of Endocrinology and Metabolism, Department of Internal Medicine, UT Southwestern Medical Center, Dallas, Texas 75390 8857, USA
    Semin Reprod Med 22:281-8. 2004
  5. ncbi request reprint Miscellaneous endocrine causes of hypertension
    Richard J Auchus
    Division of Endocrinology and Metabolism, Department of Internal Medicine, and Donald W Reynolds Cardiovascular Clinical Research Center, University of Texas Southwestern Medical School, Dallas, TX 75390 8857, USA
    Curr Cardiol Rep 7:418-24. 2005
  6. ncbi request reprint Primary aldosteronism
    Richard J Auchus
    Division of Endocrinology and Metabolism, UT Southwestern Medical Center, Dallas, TX 75390, USA
    Curr Cardiol Rep 9:447-52. 2007
  7. doi request reprint 46,XX DSD: the masculinised female
    Richard J Auchus
    Division of Endocrinology and Metabolism, Department of Internal Medicine, UT Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX 75390 8857, United States
    Best Pract Res Clin Endocrinol Metab 24:219-42. 2010
  8. ncbi request reprint Measurement of 18-hydroxycorticosterone during adrenal vein sampling for primary aldosteronism
    Richard J Auchus
    Division of Endocrinology and Metabolism, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, Texas 75390 8857, USA
    J Clin Endocrinol Metab 92:2648-51. 2007
  9. doi request reprint Non-traditional metabolic pathways of adrenal steroids
    Richard J Auchus
    Division of Endocrinology and Metabolism, Department of Internal Medicine, UT Southwestern Medical Center, 5323 Harry Hines Blvd, Box 8857, Dallas, TX 75390 8857, USA
    Rev Endocr Metab Disord 10:27-32. 2009
  10. doi request reprint Rapid cortisol assays improve the success rate of adrenal vein sampling for primary aldosteronism
    Richard J Auchus
    Department of Internal Medicine, UT Southwestern Medical Center, Dallas, Texas 75390 8857, USA
    Ann Surg 249:318-21. 2009

Collaborators

Detail Information

Publications62

  1. ncbi request reprint Aldo is back: recent advances and unresolved controversies in hyperaldosteronism
    Richard J Auchus
    Division of Endocrinology and Metabolism, UT Southwestern Medical Center, Dallas, Texas 75390, USA
    Curr Opin Nephrol Hypertens 12:153-8. 2003
    ....
  2. doi request reprint Congenital adrenal hyperplasia in adults
    Richard J Auchus
    Division of Endocrinology and Metabolism, Department of Internal Medicine, UT Southwestern Medical Center, Dallas, Texas 75390 8857, USA
    Curr Opin Endocrinol Diabetes Obes 17:210-6. 2010
    ..This success has created a new clinical entity, for which the natural history and optimal management strategies are not known...
  3. doi request reprint Primary aldosteronism and a Texas two-step
    Richard J Auchus
    Division of Endocrinology and Metabolism, UT Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX 75390 8857, USA
    Rev Endocr Metab Disord 12:37-42. 2011
    ..This review discusses the approach we have taken in Dallas, a strategy of targeted screening, referral for positive screens, and individualized management...
  4. ncbi request reprint Overview of dehydroepiandrosterone biosynthesis
    Richard J Auchus
    Division of Endocrinology and Metabolism, Department of Internal Medicine, UT Southwestern Medical Center, Dallas, Texas 75390 8857, USA
    Semin Reprod Med 22:281-8. 2004
    ..These characteristics are illustrated by the fetal adrenal cortex and the zona reticularis, which are dedicated to the synthesis of DHEA and DHEA-sulfate...
  5. ncbi request reprint Miscellaneous endocrine causes of hypertension
    Richard J Auchus
    Division of Endocrinology and Metabolism, Department of Internal Medicine, and Donald W Reynolds Cardiovascular Clinical Research Center, University of Texas Southwestern Medical School, Dallas, TX 75390 8857, USA
    Curr Cardiol Rep 7:418-24. 2005
    ..This review focuses on uncommon forms of ACTH-dependent mineralocorticoid excess states associated with hypertension...
  6. ncbi request reprint Primary aldosteronism
    Richard J Auchus
    Division of Endocrinology and Metabolism, UT Southwestern Medical Center, Dallas, TX 75390, USA
    Curr Cardiol Rep 9:447-52. 2007
    ..This review uses illustrative examples to describe our approach to these patients...
  7. doi request reprint 46,XX DSD: the masculinised female
    Richard J Auchus
    Division of Endocrinology and Metabolism, Department of Internal Medicine, UT Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX 75390 8857, United States
    Best Pract Res Clin Endocrinol Metab 24:219-42. 2010
    ..This review discusses these diseases, their pathogenesis and approaches to therapy. We emphasise areas where improved treatments are sorely needed...
  8. ncbi request reprint Measurement of 18-hydroxycorticosterone during adrenal vein sampling for primary aldosteronism
    Richard J Auchus
    Division of Endocrinology and Metabolism, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, Texas 75390 8857, USA
    J Clin Endocrinol Metab 92:2648-51. 2007
    ....
  9. doi request reprint Non-traditional metabolic pathways of adrenal steroids
    Richard J Auchus
    Division of Endocrinology and Metabolism, Department of Internal Medicine, UT Southwestern Medical Center, 5323 Harry Hines Blvd, Box 8857, Dallas, TX 75390 8857, USA
    Rev Endocr Metab Disord 10:27-32. 2009
    ..This chapter will consider alternate fates encountered by steroid hormone precursors in the adrenal gland, the variables influencing flux through these secondary pathways, and the significance of these diversions in health and disease...
  10. doi request reprint Rapid cortisol assays improve the success rate of adrenal vein sampling for primary aldosteronism
    Richard J Auchus
    Department of Internal Medicine, UT Southwestern Medical Center, Dallas, Texas 75390 8857, USA
    Ann Surg 249:318-21. 2009
    ..We hypothesized that an adrenal vein sampling (AVS) algorithm incorporating rapid cortisol assays, which enables resampling of the adrenal veins, would improve the success rate by a team of radiologists...
  11. doi request reprint What we still do not know about adrenal vein sampling for primary aldosteronism
    R J Auchus
    Division of Endocrinology and Metabolism, Department of Internal Medicine, UT Southwestern Medical Center, Dallas, TX 75390 8857, USA
    Horm Metab Res 42:411-5. 2010
    ....
  12. doi request reprint The life and scientific contributions of Keith L. Parker, 1953-2008
    Richard J Auchus
    Division of Endocrinology and Metabolism, Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390 8857, United States
    Mol Cell Endocrinol 336:191-2. 2011
    ..In this article, we briefly review his life, accomplishments, and legacy...
  13. doi request reprint The physiology and biochemistry of adrenarche
    Richard J Auchus
    Division of Endocrinology and Metabolism, Department of Internal Medicine, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX 75390, USA
    Endocr Dev 20:20-7. 2011
    ..The purpose of adrenarche in human physiology remains enigmatic, although disease states associated with abnormalities in DHEA(S) production provide some clues...
  14. ncbi request reprint The backdoor pathway to dihydrotestosterone
    Richard J Auchus
    Division of Endocrinology and Metabolism, Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX, USA
    Trends Endocrinol Metab 15:432-8. 2004
    ....
  15. ncbi request reprint The function and roles of P450c17 in androgen excess states
    R J Auchus
    Department of Internal Medicine, UT Southwestern School of Medicine, Dallas, TX 75390 8857, USA
    J Pediatr Endocrinol Metab 13:1271-5. 2000
    ..This paper explores the function of P450c17 in its proper biological context, using biochemical studies of patients with isolated 17,20-lyase activity to demonstrate key concepts in P450c17 enzymology...
  16. ncbi request reprint The genetics, pathophysiology, and management of human deficiencies of P450c17
    R J Auchus
    Division of Endocrinology and Metabolism, Department of Internal Medicine, University of Texas Southwestern Medical School, Dallas, Texas, USA
    Endocrinol Metab Clin North Am 30:101-19, vii. 2001
    ..This article reviews the genetics and biochemistry of P450c17 as a prelude for understanding the pathophysiology of these diseases and approaches to their diagnosis and management...
  17. ncbi request reprint Adrenarche - physiology, biochemistry and human disease
    Richard J Auchus
    Division of Endocrinology and Metabolism, Department of Internal Medicine, UT Southwestern Medical Center, Dallas, TX, USA
    Clin Endocrinol (Oxf) 60:288-96. 2004
    ..This article reviews the clinical manifestations of adrenarche, the biochemistry of the enzymes involved in DHEA-S production, and the cell biology of the adrenal zona reticularis...
  18. ncbi request reprint The enantiomer of progesterone (ent-progesterone) is a competitive inhibitor of human cytochromes P450c17 and P450c21
    Richard J Auchus
    Division of Endocrinology and Metabolism, Department of Internal Medicine, University of Texas Southwestern Medical Center, 5323 Harry Hines Bovlevard, Dallas 75390 8857, USA
    Arch Biochem Biophys 409:134-44. 2003
    ..Enantiomeric steroids can be used to probe steroid binding sites, and these compounds may be effective inhibitors of steroid biosynthesis...
  19. ncbi request reprint Towards a unifying mechanism for CYP17 mutations that cause isolated 17,20-lyase deficiency
    Richard J Auchus
    Division of Endocrinology and Metabolism, Department of Internal Medicine, UT Southwestern Medical Center, Dallas, TX 75390 8857, USA
    Endocr Res 28:443-7. 2002
    ....
  20. ncbi request reprint Primary hyperaldosteronism: effect of adrenal vein sampling on surgical outcome
    Fiemu E Nwariaku
    Department of Surgery, University of Texas Southwestern Medical Center, Dallas 75390 9156, USA
    Arch Surg 141:497-502; discussion 502-3. 2006
    ..Adrenal vein sampling is superior to computed tomography for subtype differentiation of primary hyperaldosteronism...
  21. ncbi request reprint 5alpha-reduced C21 steroids are substrates for human cytochrome P450c17
    Manisha K Gupta
    Division of Endocrinology and Metabolism, Department of Internal Medicine, 5323 Harry Hines Blvd, Dallas, TX 75390 8857, USA
    Arch Biochem Biophys 418:151-60. 2003
    ....
  22. doi request reprint "Getting from here to there"--mechanisms and limitations to the activation of the androgen receptor in castration-resistant prostate cancer
    Nima Sharifi
    Divisions of Hematology and Oncology, Department of Internal Medicine, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX 75390, USA
    J Investig Med 58:938-44. 2010
    ....
  23. ncbi request reprint Associations among androgens, estrogens, and natriuretic peptides in young women: observations from the Dallas Heart Study
    Alice Y Chang
    The Donald W Reynolds Cardiovascular Clinical Research Center and the Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9066, USA
    J Am Coll Cardiol 49:109-16. 2007
    ..We sought to determine if natriuretic peptides are associated with estrogen and androgen status in a population study of young women without known cardiac disease...
  24. ncbi request reprint The rise in adrenal androgen biosynthesis: adrenarche
    Jon C Havelock
    Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, UT Southwestern Medical Center at Dallas, Dallas, Texas 75390 9032, USA
    Semin Reprod Med 22:337-47. 2004
    ..In addition, children with premature and exaggerated adrenarche may be predisposed to certain diseases later in life...
  25. doi request reprint Polycystic ovarian syndrome and subclinical atherosclerosis among women of reproductive age in the Dallas heart study
    Alice Y Chang
    The Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, USA
    Clin Endocrinol (Oxf) 74:89-96. 2011
    ..We sought to determine in a multiethnic population-based sample whether women with PCOS have greater measures of subclinical atherosclerosis than women without PCOS...
  26. ncbi request reprint Basic concepts and recent developments in human steroid hormone biosynthesis
    Hans K Ghayee
    Division of Endocrinology and Metabolism, Department of Internal Medicine, UT Southwestern Medical Center, Dallas, TX 75390 8857, USA
    Rev Endocr Metab Disord 8:289-300. 2007
    ..We will conclude with a description of aberrant signaling mechanisms observed in adrenal tumors as a further example of how these pathways can be disturbed in pathologic states...
  27. ncbi request reprint Human cytochrome b5 requires residues E48 and E49 to stimulate the 17,20-lyase activity of cytochrome P450c17
    Jacqueline L Naffin-Olivos
    Division of Endocrinology and Metabolism, Department of Internal Medicine, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, Texas 75390 8857, USA
    Biochemistry 45:755-62. 2006
    ..These data suggest that a specific allosteric binding site on b5, which includes residues E48, E49, and possibly R52, mediates the stimulation of 17,20-lyase activity...
  28. ncbi request reprint CYP17 mutation E305G causes isolated 17,20-lyase deficiency by selectively altering substrate binding
    Daniel P Sherbet
    Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, Texas 75390 8857, USA
    J Biol Chem 278:48563-9. 2003
    ..Mutation E305G provides genetic evidence that androstenedione formation from 17alpha-hydroxyprogesterone via the minor delta4-steroid pathway alone is not sufficient for complete formation of the male phenotype in humans...
  29. ncbi request reprint Steroid 5alpha-reductase 1 promotes 5alpha-androstane-3alpha,17beta-diol synthesis in immature mouse testes by two pathways
    Mala Mahendroo
    Department of Obstetrics and Gynecology, The University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, TX 75390 9032, USA
    Mol Cell Endocrinol 222:113-20. 2004
    ....
  30. pmc Differential effects of chlorthalidone versus spironolactone on muscle sympathetic nerve activity in hypertensive patients
    Dileep V Menon
    Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, Texas 75390 8586, USA
    J Clin Endocrinol Metab 94:1361-6. 2009
    ....
  31. doi request reprint Clinical implications of androgen synthesis via 5alpha-reduced precursors
    Hans K Ghayee
    Division of Endocrinology and Metabolism, Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390 8857, USA
    Endocr Dev 13:55-66. 2008
    ..A better understanding of the 5alpha-reduced or 'backdoor'pathway to DHT in human disorders of androgen excess will provide pharmacotherapy opportunities to effectively treat androgen excess in females...
  32. pmc Menstrual cycle affects renal-adrenal and hemodynamic responses during prolonged standing in the postural orthostatic tachycardia syndrome
    Qi Fu
    Institute for Exercise and Environmental Medicine, Texas Health Presbyterian Hospital Dallas, Dallas, Tex 75231, USA
    Hypertension 56:82-90. 2010
    ..The high estrogen and progesterone in the MLP are associated with greater increases in renal-adrenal hormones and presumably more volume retention, which improve late-standing tolerance in these patients...
  33. ncbi request reprint "Subclinical Cushing's syndrome" is not subclinical: improvement after adrenalectomy in 9 patients
    Ian C Mitchell
    Division of Gastrointestinal and Endocrine Surgery, Department of Surgery, University of Texas Southwestern Medical Center, Dallas, TX 75390 9156, USA
    Surgery 142:900-5; discussion 905.e1. 2007
    ..Appropriate therapy for this entity, subclinical Cushing's syndrome (subclinical CS), is unclear. We examined outcomes for patients who underwent unilateral adrenalectomy for subclinical CS...
  34. ncbi request reprint Human 17beta-hydroxysteroid dehydrogenases types 1, 2, and 3 catalyze bi-directional equilibrium reactions, rather than unidirectional metabolism, in HEK-293 cells
    Naveed Khan
    Divison of Reproductive Sciences, Department of Obstetrics and Gynecology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX 75390 8857, USA
    Arch Biochem Biophys 429:50-9. 2004
    ..These 17betaHSD isoforms do not drive steroid flux in one direction but rather may achieve functional equilibria in intact cells, reflecting thermodynamically driven steroid distributions...
  35. doi request reprint 3beta-hydroxysteroid dehydrogenase is a possible pharmacological target in the treatment of castration-resistant prostate cancer
    Kristen Evaul
    Division of Hematology and Oncology, Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, Texas 75390 8852, USA
    Endocrinology 151:3514-20. 2010
    ..These findings demonstrate that DHEA and A5diol must be metabolized by 3betaHSD to activate AR in these models of CRPC. Furthermore, this work suggests that 3betaHSD may be exploited as a pharmacologic target in the treatment of CRPC...
  36. pmc Reversible sympathetic overactivity in hypertensive patients with primary aldosteronism
    Andrew C Kontak
    Cardiology Division, University of Texas Southwestern Medical Center, Dallas, Texas 75390 8586, USA
    J Clin Endocrinol Metab 95:4756-61. 2010
    ..Aldosterone has been shown to exert a central sympathoexcitatory action in multiple animal models, but evidence in humans is still lacking...
  37. ncbi request reprint Minireview: cellular redox state regulates hydroxysteroid dehydrogenase activity and intracellular hormone potency
    Anil K Agarwal
    Division of Endocrinology and Metabolism, Department of Internal Medicine, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, Texas 75390 8857, USA
    Endocrinology 146:2531-8. 2005
    ..We will review the evidence linking cofactor handling and HSD activity, speculate on additional ways that intracellular metabolism can alter HSD activity and, thus, hormone potency, and discuss fruitful avenues of further investigation...
  38. ncbi request reprint Deoxycorticosterone inactivation by AKR1C3 in human mineralocorticoid target tissues
    Kamalesh K Sharma
    Department of Obstetrics Gynecology and Biochemistry, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Mol Cell Endocrinol 248:79-86. 2006
    ..Our findings suggest that AKR1C3 protects the mineralocorticoid receptor from activation by DOC in mineralocorticoid target cells of the kidney and colon, analogous to cortisol inactivation by 11beta-hydroxysteroid dehydrogenase type 2...
  39. pmc Clinical, biochemical, and molecular characterization of macronodular adrenocortical hyperplasia of the zona reticularis: a new syndrome
    Hans K Ghayee
    Division of Endocrinology and Metabolism, Department of Internal Medicine, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, Texas 75390 8857, USA
    J Clin Endocrinol Metab 96:E243-50. 2011
    ..We describe a 29-yr-old man with massive macronodular adrenocortical hyperplasia without hypercortisolemia but rather markedly elevated and nonsuppressible production of dehydroepiandrosterone (DHEA) and its sulfate (DHEAS)...
  40. doi request reprint Androgens, estrogens, and hydroxysteroid dehydrogenases
    Dario Mizrachi
    Divisions of Endocrinology and Metabolism, Department of Internal Medicine, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390 8857, USA
    Mol Cell Endocrinol 301:37-42. 2009
    ....
  41. pmc Identification of the nuclear receptor DAF-12 as a therapeutic target in parasitic nematodes
    Zhu Wang
    Department of Pharmacology and Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Proc Natl Acad Sci U S A 106:9138-43. 2009
    ..stercoralis DAF-12 ligand-binding domain cocrystallized with dafachronic acids. These results reveal the molecular basis for DAF-12 ligand binding and identify nuclear receptors as unique therapeutic targets in parasitic nematodes...
  42. ncbi request reprint Epithelial sodium channel allele T594M is not associated with blood pressure or blood pressure response to amiloride
    John M Hollier
    Division of Hypertension, Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390 8857, USA
    Hypertension 47:428-33. 2006
    ..8). We conclude that the T594M allele does not contribute significantly to BP in blacks and does not predict a significantly superior response to amiloride therapy...
  43. ncbi request reprint Arginine 276 controls the directional preference of AKR1C9 (rat liver 3alpha-hydroxysteroid dehydrogenase) in human embryonic kidney 293 cells
    Mahboubeh Papari-Zareei
    Division of Endocrinology and Metabolism, Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, 75390 8857, USA
    Endocrinology 147:1591-7. 2006
    ..We conclude the R276 maximizes the reductive preference of AKR1C9 in intact cells and maintains this strong preference despite NADPH depletion; mutation R276E reverses the directional preference...
  44. pmc Biochemical factors governing the steady-state estrone/estradiol ratios catalyzed by human 17beta-hydroxysteroid dehydrogenases types 1 and 2 in HEK-293 cells
    Daniel P Sherbet
    Division of Endocrinology and Metabolism, Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, Texas 75390 8857, USA
    Endocrinology 150:4154-62. 2009
    ..These findings suggest that additional structural features, beyond the lack of a specific arginine residue, disfavor NADPH binding and thus support E2 oxidation by 17betaHSD2 in intact cells...
  45. ncbi request reprint Dehydroepiandrosterone replacement therapy--panacea, snake oil, or a bit of both?
    Alice Y Chang
    Division of Endocrinology and Metabolism, University of Texas Southwestern Medical Center, Dallas, TX 75390 8857, USA
    Nat Clin Pract Endocrinol Metab 4:442-3. 2008
  46. ncbi request reprint Do allelic variants in alpha2A and alpha2C adrenergic receptors predispose to hypertension in blacks?
    Jia Ling Li
    Division of Hypertension, Department of Internal Medicine, Donald W Reynolds Cardiovascular Clinical Research Center, University of Texas Southwestern Medical Center, Dallas, USA
    Hypertension 47:1140-6. 2006
    ..Both the DraI restriction fragment length polymorphism in ADRA2A and the ADRA2C (Del 322 to 325) can be excluded as major candidate alleles for hypertension in blacks...
  47. ncbi request reprint Cofactors, redox state, and directional preferences of hydroxysteroid dehydrogenases
    Daniel P Sherbet
    Division of Endocrinology and Metabolism, Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390 8857, United States
    Mol Cell Endocrinol 265:83-8. 2007
    ....
  48. ncbi request reprint Comparison of the hamster and human adrenal P450c17 (17 alpha-hydroxylase/17,20-lyase) using site-directed mutagenesis and molecular modeling
    Axel P Mathieu
    Department of Biochemistry, Faculty of Medicine, University of, Sherbrooke, Que, Canada J1H 5N4
    J Steroid Biochem Mol Biol 80:99-107. 2002
    ..By analogy, these results support the concept that any cellular changes modifying the external structure of P450c17, such as phosphorylation, could have influence on its active site and enzymatic activities...
  49. ncbi request reprint The 17, 20-lyase activity of cytochrome p450c17 from human fetal testis favors the delta5 steroidogenic pathway
    Christa E Flück
    Department of Pediatrics and Metabolic Research Unit, University of California, San Francisco, California 94143 0978, USA
    J Clin Endocrinol Metab 88:3762-6. 2003
    ..We conclude that the majority of testosterone biosynthesis in the human testis proceeds through the conversion of pregnenolone to dehydroepiandrosterone via the delta(5) pathway...
  50. ncbi request reprint Molecular evolution of adrenarche: structural and functional analysis of p450c17 from four primate species
    Wiebke Arlt
    Department of Pediatrics and the Metabolic Research Unit, University of California, San Francisco, California 94143 0978, USA
    Endocrinology 143:4665-72. 2002
    ..These data establish that Arg255 is important for 17alpha-hydroxylase activity and show that the evolution of adrenarche in higher primates is not determined by variations in the sequence of P450c17...
  51. ncbi request reprint Two intronic mutations cause 17-hydroxylase deficiency by disrupting splice acceptor sites: direct demonstration of aberrant splicing and absent enzyme activity by expression of the entire CYP17 gene in HEK-293 cells
    Marivania Costa-Santos
    Division of Endocrinology and Metabolism, Department of Medicine, Escola Paulista de Medicina, Federal University of Sao Paulo, Sao Paulo, Brazil 04039 034
    J Clin Endocrinol Metab 89:43-8. 2004
    ..These studies also demonstrate the feasibility of expressing the entire CYP17 gene, with simultaneous protein and RNA analysis, as a general methodology for characterizing how intronic CYP17 mutations cause 17OHD...
  52. ncbi request reprint Ontogeny and pathway of formation of 5alpha-androstane-3alpha,17beta-diol in the testes of the immature brushtail possum Trichosurus vulpecula
    Jean D Wilson
    Department of Zoology, University of Melbourne, Victoria, Australia
    Reprod Fertil Dev 17:603-9. 2005
    ....
  53. ncbi request reprint Will the lessons from primary aldosteronism change the treatment of hypertension and left ventricular hypertrophy?
    Richard J Auchus
    Hypertension 50:837-9. 2007
  54. ncbi request reprint Role of the alternate pathway of dihydrotestosterone formation in virilization of the Wolffian ducts of the tammar wallaby, Macropus eugenii
    Geoffrey Shaw
    Department of Zoology, The University of Melbourne, Victoria, Australia
    Endocrinology 147:2368-73. 2006
    ..We conclude that dihydrotestosterone, largely formed in the tissue by the oxidation of androstanediol derived from the testes and also the 5alpha-reduction of testosterone, is responsible for Wolffian duct virilization in this species...
  55. ncbi request reprint Novel mutation in cytochrome P450c17 causes complete combined 17alpha-hydroxylase/17,20-lyase deficiency
    Amrit Bhangoo
    Division of Pediatric Endocrinology, Infants and Children s Hospital of Brooklyn at Maimonides, Brooklyn, NY, USA
    J Pediatr Endocrinol Metab 21:185-90. 2008
    ..We describe a case of complete combined 17OHD caused by mutations in the CYP17 gene...
  56. ncbi request reprint Molecular dynamics of substrate complexes with hamster cytochrome P450c17 (CYP17): mechanistic approach to understanding substrate binding and activities
    Axel P Mathieu
    Department of Biochemistry, Faculty of Medicine, University of Sherbrooke, QC, J1H 5N4, Sherbrooke, Canada
    Biochim Biophys Acta 1619:291-300. 2003
    ....
  57. ncbi request reprint Two prevalent CYP17 mutations and genotype-phenotype correlations in 24 Brazilian patients with 17-hydroxylase deficiency
    Marivania Costa-Santos
    Division of Endocrinology and Metabolism, Department of Medicine, Escola Paulista de Medicina, Federal University of Sao Paulo, Sao Paulo, Brazil 04039 034
    J Clin Endocrinol Metab 89:49-60. 2004
    ..Spontaneous sexual development occurred only in 46,XX females with partial deficiencies. We conclude that other factors, in addition to CYP17 genotype, contribute to the phenotype of individual patients with 17-hydroxylase deficiency...
  58. ncbi request reprint Position statement: Utility, limitations, and pitfalls in measuring testosterone: an Endocrine Society position statement
    William Rosner
    St Luke s Roosevelt Hospital Center, 1000 Tenth Avenue, AJA 403, New York, New York 10019, USA
    J Clin Endocrinol Metab 92:405-13. 2007
    ..The objective of the study was to evaluate the current state of clinical assays for total and free testosterone...
  59. pmc Variable phenotypes associated with aromatase (CYP19) insufficiency in humans
    Lin Lin
    Developmental Endocrinology Research Group, Clinical and Molecular Genetics Unit, UCL Institute of Child Health, University College London, 30 Guilford Street, London WC1N 1EH, United Kingdom
    J Clin Endocrinol Metab 92:982-90. 2007
    ..Complete aromatase deficiency has been reported in a small number of 46,XX girls with genital ambiguity and absent pubertal development, but it is unknown whether nonclassic phenotypes exist...
  60. ncbi request reprint Testosterone supplementation of megestrol therapy does not enhance lean tissue accrual in men with human immunodeficiency virus-associated weight loss: a randomized, double-blind, placebo-controlled, multicenter trial
    Kathleen Mulligan
    Department of Medicine, University of California, San Francisco, California 94110, USA
    J Clin Endocrinol Metab 92:563-70. 2007
    ..Reduced energy intake is a primary factor in HIV-associated wasting. Megestrol acetate (MA) stimulates appetite and weight gain. However, much of the weight gained is fat, possibly as a result of MA-induced hypogonadism...
  61. pmc Androstenedione up-regulation of endometrial aromatase expression via local conversion to estrogen: potential relevance to the pathogenesis of endometriosis
    Orhan Bukulmez
    Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology and Infertility, University of Florida College of Medicine, 1600 SW Archer Road, Gainesville, Florida 32610 0294, USA
    J Clin Endocrinol Metab 93:3471-7. 2008
    ..The factors that mediate induction of aromatase in the endometrium are not well defined, but increased expression of steroidogenic factor (SF)-1 may play a role...
  62. ncbi request reprint 5alpha-androstane-3alpha,17beta-diol is formed in tammar wallaby pouch young testes by a pathway involving 5alpha-pregnane-3alpha,17alpha-diol-20-one as a key intermediate
    Jean D Wilson
    Department of Zoology, University of Melbourne, Victoria 3010, Australia
    Endocrinology 144:575-80. 2003
    ..We conclude that expression of steroid 5alpha-reductase in the developing wallaby testes allows formation of 5alpha-reduced androgens by a pathway that does not involve testosterone as an intermediate...

Research Grants3

  1. Fusion Proteins As Probes of P450 Structure and Function
    Richard Auchus; Fiscal Year: 2004
    ..In addition, this approach might be generally applicable to structural studies of membrane-bound proteins. ..
  2. Activation of androgen biosynthesis and drug metabolism by cytochrome b5
    Richard J Auchus; Fiscal Year: 2010
    ..This knowledge will provide a novel approach for suppressing androgen production, by targeting the CYP17A1-b5 interaction. ..