DAVID AUBLE

Summary

Affiliation: University of Virginia
Country: USA

Publications

  1. pmc Molecular analysis of the SNF2/SWI2 protein family member MOT1, an ATP-driven enzyme that dissociates TATA-binding protein from DNA
    D T Auble
    Department of Biochemistry, University of Virginia Health Science Center, Charlottesville 22908, USA
    Mol Cell Biol 17:4842-51. 1997
  2. pmc Testing for DNA tracking by MOT1, a SNF2/SWI2 protein family member
    D T Auble
    Department of Biochemistry and Molecular Genetics, University of Virginia Health Science Center, Charlottesville, Virginia 22908, USA
    Mol Cell Biol 19:412-23. 1999
  3. pmc The dynamic personality of TATA-binding protein
    David T Auble
    Department of Biochemistry and Molecular Genetics, University of Virginia Health System, Charlottesville, VA 22908, USA
    Trends Biochem Sci 34:49-52. 2009
  4. pmc Regulation of rRNA synthesis by TATA-binding protein-associated factor Mot1
    Arindam Dasgupta
    Department of Biochemistry and Molecular Genetics, University of Virginia Health System, 1300 Jefferson Park Avenue, Charlottesville, Virginia 22908 0733, USA
    Mol Cell Biol 27:2886-96. 2007
  5. pmc Mot1 activates and represses transcription by direct, ATPase-dependent mechanisms
    Arindam Dasgupta
    Department of Biochemistry and Molecular Genetics, University of Virginia Health System, 1300 Jefferson Park Avenue, Room 6213, Charlottesville, VA 22908 0733, USA
    Proc Natl Acad Sci U S A 99:2666-71. 2002
  6. pmc Mot1-mediated control of transcription complex assembly and activity
    Arindam Dasgupta
    Department of Biochemistry and Molecular Genetics, University of Virginia Health System, Charlottesville, VA 22908 0733, USA
    EMBO J 24:1717-29. 2005
  7. pmc Function and structural organization of Mot1 bound to a natural target promoter
    Rebekka O Sprouse
    Department of Biochemistry and Molecular Genetics, University of Virginia Health System, Charlottesville, Virginia 22908, USA
    J Biol Chem 283:24935-48. 2008
  8. pmc TATA-binding protein variants that bypass the requirement for Mot1 in vivo
    Rebekka O Sprouse
    Department of Biochemistry and Molecular Genetics, University of Virginia Health System, Charlottesville, Virginia 22908, USA
    J Biol Chem 284:4525-35. 2009
  9. pmc Snf2/Swi2-related ATPase Mot1 drives displacement of TATA-binding protein by gripping DNA
    Rebekka O Sprouse
    Department of Biochemistry and Molecular Genetics, University of Virginia Health System, Charlottesville, VA 22908 0733, USA
    EMBO J 25:1492-504. 2006
  10. ncbi request reprint Genome-wide analysis of ARS (autonomously replicating sequence) binding factor 1 (Abf1p)-mediated transcriptional regulation in Saccharomyces cerevisiae
    Tsuyoshi Miyake
    Department of Biochemistry and Molecular Genetics, School of Medicine, University of Virginia, Charlottesville, VA 22908 0733, USA
    J Biol Chem 279:34865-72. 2004

Collaborators

  • JEFFREY SCOTT SMITH
  • S Hahn
  • D Wang
  • T Miyake
  • Patrick Grant
  • R Li
  • James G McNally
  • Rebekka O Sprouse
  • Arindam Dasgupta
  • Kerrington L Ramsey
  • Russell P Darst
  • Staton L Wade
  • Michael Brenowitz
  • Sarah A Juedes
  • Melissa N Wells
  • Stefan Bekiranov
  • Kunal Poorey
  • Huiyong Cheng
  • Florian Mueller
  • Inna Shcherbakova
  • Tatiana S Karpova
  • Elizabeth Jamison
  • Robert Hontz
  • Sarah French
  • Pavel Aprikian
  • Ann L Beyer
  • Nazif Maqani
  • Jer Yuan Hsu
  • Amy Vroom
  • Chunming Zhu
  • Tamara Muldrow
  • Karla J Martin
  • Cynthia A Afshari

Detail Information

Publications15

  1. pmc Molecular analysis of the SNF2/SWI2 protein family member MOT1, an ATP-driven enzyme that dissociates TATA-binding protein from DNA
    D T Auble
    Department of Biochemistry, University of Virginia Health Science Center, Charlottesville 22908, USA
    Mol Cell Biol 17:4842-51. 1997
    ..These results provide a general framework for understanding how members of the SNF2/SWI2 protein family use ATP to modulate protein-DNA interactions to regulate many diverse processes in cells...
  2. pmc Testing for DNA tracking by MOT1, a SNF2/SWI2 protein family member
    D T Auble
    Department of Biochemistry and Molecular Genetics, University of Virginia Health Science Center, Charlottesville, Virginia 22908, USA
    Mol Cell Biol 19:412-23. 1999
    ..These results support a model in which MOT1 drives TBP-DNA dissociation by a mechanism that involves a transient, ATP-dependent interaction with TBP-DNA which does not involve ATP-dependent DNA tracking...
  3. pmc The dynamic personality of TATA-binding protein
    David T Auble
    Department of Biochemistry and Molecular Genetics, University of Virginia Health System, Charlottesville, VA 22908, USA
    Trends Biochem Sci 34:49-52. 2009
    ....
  4. pmc Regulation of rRNA synthesis by TATA-binding protein-associated factor Mot1
    Arindam Dasgupta
    Department of Biochemistry and Molecular Genetics, University of Virginia Health System, 1300 Jefferson Park Avenue, Charlottesville, Virginia 22908 0733, USA
    Mol Cell Biol 27:2886-96. 2007
    ....
  5. pmc Mot1 activates and represses transcription by direct, ATPase-dependent mechanisms
    Arindam Dasgupta
    Department of Biochemistry and Molecular Genetics, University of Virginia Health System, 1300 Jefferson Park Avenue, Room 6213, Charlottesville, VA 22908 0733, USA
    Proc Natl Acad Sci U S A 99:2666-71. 2002
    ..The gene sets controlled by NC2 and Srb10 are strongly correlated with the Mot1-controlled set, suggesting that these factors cooperate in transcriptional control on a global scale...
  6. pmc Mot1-mediated control of transcription complex assembly and activity
    Arindam Dasgupta
    Department of Biochemistry and Molecular Genetics, University of Virginia Health System, Charlottesville, VA 22908 0733, USA
    EMBO J 24:1717-29. 2005
    ..We suggest that at activated promoters, Mot1 disassembles transcriptionally inactive TBP, thereby facilitating the formation of a TBP complex that supports functional PIC assembly...
  7. pmc Function and structural organization of Mot1 bound to a natural target promoter
    Rebekka O Sprouse
    Department of Biochemistry and Molecular Genetics, University of Virginia Health System, Charlottesville, Virginia 22908, USA
    J Biol Chem 283:24935-48. 2008
    ..The results suggest that Mot1-mediated activation of URA1 transcription involves at least two steps, one of which is the removal of TBP bound to the promoter in the opposite orientation required for URA1 transcription...
  8. pmc TATA-binding protein variants that bypass the requirement for Mot1 in vivo
    Rebekka O Sprouse
    Department of Biochemistry and Molecular Genetics, University of Virginia Health System, Charlottesville, Virginia 22908, USA
    J Biol Chem 284:4525-35. 2009
    ..The results also suggest that PICs can be dynamically unstable and that appropriate PIC instability is critical for the regulation of transcription in vivo...
  9. pmc Snf2/Swi2-related ATPase Mot1 drives displacement of TATA-binding protein by gripping DNA
    Rebekka O Sprouse
    Department of Biochemistry and Molecular Genetics, University of Virginia Health System, Charlottesville, VA 22908 0733, USA
    EMBO J 25:1492-504. 2006
    ..We also report a novel ATP-independent TBPc displacement activity for Mot1 and describe conformational heterogeneity in the Mot1 ATPase, which is likely a general feature of other enzymes in this class...
  10. ncbi request reprint Genome-wide analysis of ARS (autonomously replicating sequence) binding factor 1 (Abf1p)-mediated transcriptional regulation in Saccharomyces cerevisiae
    Tsuyoshi Miyake
    Department of Biochemistry and Molecular Genetics, School of Medicine, University of Virginia, Charlottesville, VA 22908 0733, USA
    J Biol Chem 279:34865-72. 2004
    ..Taken together, these results strongly suggest distinct underlying mechanisms by which Abf1p regulates gene expression...
  11. ncbi request reprint Mot1 regulates the DNA binding activity of free TATA-binding protein in an ATP-dependent manner
    Russell P Darst
    Department of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville, Virginia 22908, USA
    J Biol Chem 278:13216-26. 2003
    ..A model for Mot1 action is proposed in which ATP hydrolysis causes the Mot1 N terminus to displace the TATA box, leading to ejection of Mot1 and TBP from DNA...
  12. ncbi request reprint Sir Antagonist 1 (San1) is a ubiquitin ligase
    Arindam Dasgupta
    Department of Biochemistry and Molecular Genetics, University of Virginia Health System, Charlottesville, Virginia 22908 0733, USA
    J Biol Chem 279:26830-8. 2004
    ....
  13. pmc The Snf1 kinase and proteasome-associated Rad23 regulate UV-responsive gene expression
    Staton L Wade
    Department of Biochemistry and Molecular Genetics, University of Virginia Health System, Charlottesville, VA 22908 0733, USA
    EMBO J 28:2919-31. 2009
    ..These results also highlight how diverse environmental stimuli are processed by a limited repertoire of signalling molecules to result in tailored patterns of gene expression...
  14. pmc The NEF4 complex regulates Rad4 levels and utilizes Snf2/Swi2-related ATPase activity for nucleotide excision repair
    Kerrington L Ramsey
    Department of Biochemistry and Molecular Genetics, University of Virginia Health System, Charlottesville, VA 22908 0733, USA
    Mol Cell Biol 24:6362-78. 2004
    ..As Rad23 also controls Rad4 levels, these results suggest a complex system for globally regulating repair activity in vivo by controlling turnover of Rad4...
  15. pmc Regulation of TATA-binding protein dynamics in living yeast cells
    Rebekka O Sprouse
    Department of Biochemistry and Molecular Genetics, University of Virginia Health System, 1340 Jefferson Park Avenue, Charlottesville, VA 22908, USA
    Proc Natl Acad Sci U S A 105:13304-8. 2008
    ..Our data indicate that active Pol II PICs can be much more dynamic than previously considered...

Research Grants15

  1. MOLECULAR ANALYSIS OF A YEAST TRANSCRIPTIONAL REGULATOR
    David T Auble; Fiscal Year: 2010
    ....
  2. MOLECULAR ANALYSIS OF A YEAST TRANSCRIPTIONAL REGULATOR
    DAVID AUBLE; Fiscal Year: 2007
    ....
  3. MOLECULAR ANALYSIS OF A YEAST TRANSCRIPTIONAL REGULATOR
    DAVID AUBLE; Fiscal Year: 2005
    ..The proposed analysis of Mot1 function will lead to a better understanding of the role of Motl in transcriptional control as well as a better understanding of the functions of Snf2/Swi2 family members in general. ..
  4. MOLECULAR ANALYSIS OF A YEAST TRANSCRIPTIONAL REGULATOR
    DAVID AUBLE; Fiscal Year: 2001
    ..The proposed work will also provide a framework for the analysis of other SNF2/SWI2 family members whose activities are currently poorly understood. ..
  5. MOLECULAR ANALYSIS OF A YEAST TRANSCRIPTIONAL REGULATOR
    DAVID AUBLE; Fiscal Year: 2009
    ....