Sanford Asher

Summary

Affiliation: University of Pittsburgh
Country: USA

Publications

  1. doi request reprint Two-dimensional array Debye ring diffraction protein recognition sensing
    Jian Tao Zhang
    Department of Chemistry, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Chem Commun (Camb) 49:6337-9. 2013
  2. ncbi request reprint Polymerized crystalline colloidal array chemical-sensing materials for detection of lead in body fluids
    Sanford A Asher
    Department of Chemistry, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Anal Bioanal Chem 373:632-8. 2002
  3. ncbi request reprint Photonic crystal carbohydrate sensors: low ionic strength sugar sensing
    Sanford A Asher
    Department of Chemistry, Chevron Science Center, University of Pittsburgh, Pittsburgh, PA 15260, USA
    J Am Chem Soc 125:3322-9. 2003
  4. ncbi request reprint Photonic crystal aqueous metal cation sensing materials
    Sanford A Asher
    Department of Chemistry, Chevron Science Center, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    Anal Chem 75:1676-83. 2003
  5. ncbi request reprint Diffraction in crystalline colloidal-array photonic crystals
    Sanford A Asher
    Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    Phys Rev E Stat Nonlin Soft Matter Phys 69:066619. 2004
  6. ncbi request reprint UV Raman demonstrates that alpha-helical polyalanine peptides melt to polyproline II conformations
    Sanford A Asher
    Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    J Am Chem Soc 126:8433-40. 2004
  7. doi request reprint Solid state and solution nitrate photochemistry: photochemical evolution of the solid state lattice
    Sanford A Asher
    Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, United States
    J Phys Chem A 115:4279-87. 2011
  8. ncbi request reprint Uncoupled peptide bond vibrations in alpha-helical and polyproline II conformations of polyalanine peptides
    Aleksandr V Mikhonin
    Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    J Phys Chem B 109:3047-52. 2005
  9. ncbi request reprint Photonic crystal optrode sensor for detection of Pb2+ in high ionic strength environments
    Chad E Reese
    Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    Anal Chem 75:3915-8. 2003
  10. ncbi request reprint Fast responsive crystalline colloidal array photonic crystal glucose sensors
    Matti Ben-Moshe
    Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    Anal Chem 78:5149-57. 2006

Research Grants

Collaborators

  • Eliana K Asciutto
  • CRAIG WILCOX
  • Jian Tao Zhang
  • Rob D Coalson
  • Igor K Lednev
  • E Feingold
  • Aleksandr V Mikhonin
  • Zeeshan Ahmed
  • Nataliya S Myshakina
  • David N Finegold
  • Justin T Baca
  • Sergei V Bykov
  • Bhavya Sharma
  • Justin J Bohn
  • Jeremy P Walker
  • Lu Ma
  • Kan Xiong
  • Alexander V Goponenko
  • Vladimir L Alexeev
  • Sergei Bykov
  • Xiangling Xu
  • Abdil Ozdemir
  • Matti Ben-Moshe
  • Christopher R Taormina
  • Konstantin V Pimenov
  • Kyle W Kimble
  • Chad E Reese
  • Anjal C Sharma
  • Alexander Tikhonov
  • Michelle M Ward Muscatello
  • Fangyong Yan
  • Joseph J Grabowski
  • Caroline D Geary
  • Sasmita Das
  • Anatoli Lanoul
  • Zhenmin Hong
  • Dan Qu
  • Daniel N Lamont
  • Jonathan P Scaffidi
  • Edward A Gooding
  • Jeffry D Madura
  • Lee E Stunja
  • Luling Wang
  • Adrian Murza
  • Aleksandr Mikhonin
  • Anatoli Ianoul
  • Calum Munro
  • Imants Zudans
  • Ilir A Beta
  • Marta K Maurer
  • Stephen G Weber
  • Mohamed A Virji
  • Tushar Jana
  • Lianjun Shi
  • Rasu Kesavamoorthy
  • Thomas Coleman

Detail Information

Publications55

  1. doi request reprint Two-dimensional array Debye ring diffraction protein recognition sensing
    Jian Tao Zhang
    Department of Chemistry, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Chem Commun (Camb) 49:6337-9. 2013
    ..The hydrogel volume shrinks with increasing concentrations of avidin due to the formation of avidin-biotin crosslinks. This causes the Debye diffraction ring diameter to increase, and the 2-D diffraction wavelength to blue-shift...
  2. ncbi request reprint Polymerized crystalline colloidal array chemical-sensing materials for detection of lead in body fluids
    Sanford A Asher
    Department of Chemistry, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Anal Bioanal Chem 373:632-8. 2002
    ..These IPCCA sensors can thus be used as sensing materials in optrodes to determine Pb(2+) in high-ionic-strength solutions such as body fluids...
  3. ncbi request reprint Photonic crystal carbohydrate sensors: low ionic strength sugar sensing
    Sanford A Asher
    Department of Chemistry, Chevron Science Center, University of Pittsburgh, Pittsburgh, PA 15260, USA
    J Am Chem Soc 125:3322-9. 2003
    ..This sensing material responds to glucose and other sugars at <50 microM concentrations in low ionic strength solutions...
  4. ncbi request reprint Photonic crystal aqueous metal cation sensing materials
    Sanford A Asher
    Department of Chemistry, Chevron Science Center, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    Anal Chem 75:1676-83. 2003
    ..This sensing material could be used in the field to visually determine metal cation concentrations in drinking water. A color chart would be used to relate the diffracted color to the metal cation concentration...
  5. ncbi request reprint Diffraction in crystalline colloidal-array photonic crystals
    Sanford A Asher
    Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    Phys Rev E Stat Nonlin Soft Matter Phys 69:066619. 2004
    ....
  6. ncbi request reprint UV Raman demonstrates that alpha-helical polyalanine peptides melt to polyproline II conformations
    Sanford A Asher
    Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    J Am Chem Soc 126:8433-40. 2004
    ..In addition, we estimated the Psi-angle distributions of peptide bonds which occur in non-alpha-helix and non-beta-sheet conformations in a small library of proteins...
  7. doi request reprint Solid state and solution nitrate photochemistry: photochemical evolution of the solid state lattice
    Sanford A Asher
    Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, United States
    J Phys Chem A 115:4279-87. 2011
    ..At the lowest incident fluxes a pressure buildup competes with effusion to reach a steady state giving rise to perturbed NO(3)¯ bands. Increased fluxes result in pressures that cause the sample to erupt, relieving the pressure...
  8. ncbi request reprint Uncoupled peptide bond vibrations in alpha-helical and polyproline II conformations of polyalanine peptides
    Aleksandr V Mikhonin
    Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    J Phys Chem B 109:3047-52. 2005
    ..Care must be taken in utilizing the AmI and AmI' bands for monitoring alpha-helical conformations since these bands are likely to change as the alpha-helical length changes and the backbone conformation is perturbed...
  9. ncbi request reprint Photonic crystal optrode sensor for detection of Pb2+ in high ionic strength environments
    Chad E Reese
    Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    Anal Chem 75:3915-8. 2003
    ..This transient diffraction red-shift can be used to quantitatively determine Pb2+ concentrations in high ionic strength solutions such as bodily fluids...
  10. ncbi request reprint Fast responsive crystalline colloidal array photonic crystal glucose sensors
    Matti Ben-Moshe
    Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    Anal Chem 78:5149-57. 2006
    ..We have demonstrated that the sensor is responsive to approximately 0.15 mM glucose concentrations in artificial tear fluid solution...
  11. ncbi request reprint High ionic strength glucose-sensing photonic crystal
    Vladimir L Alexeev
    Department of Chemistry, Chevron Science Center, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    Anal Chem 75:2316-23. 2003
    ..Thus, we have developed a new recognition motif for glucose that shows promise for the fabrication of noninvasive or minimally invasive in vivo glucose sensing for patients with diabetes mellitus...
  12. ncbi request reprint Cation identity dependence of crown ether photonic crystal Pb2+ sensing
    Fangyong Yan
    Department of Chemistry, Chevron Science Center, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Anal Bioanal Chem 387:2121-30. 2007
    ..We successfully modeled the response of this hydrogel Pb2+ sensor to ionic strength and the cation valence of the added salts...
  13. pmc Raman studies of solution polyglycine conformations
    Sergei Bykov
    Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    J Phys Chem B 114:6636-41. 2010
    ..This ability of Li(+) to stabilize 3(1)-helix conformations in solution gives use to the denaturing ability of lithium salts...
  14. doi request reprint Polymerized crystalline colloidal array sensing of high glucose concentrations
    Michelle M Ward Muscatello
    Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    Anal Chem 81:4978-86. 2009
    ..We examined the stability of our sensors over a period of weeks at room temperature and demonstrated that we could further stabilize our sensing materials by reversibly dehydrating them for storage...
  15. ncbi request reprint Photonic crystal sensor for organophosphate nerve agents utilizing the organophosphorus hydrolase enzyme
    Jeremy P Walker
    Department of Chemistry, Chevron Science Center, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Anal Bioanal Chem 389:2115-24. 2007
    ..The magnitude of the steady-state diffraction blueshift is proportional to the concentration of methyl paraoxon. The current detection limit is 0.2 micromol methyl paraoxon per liter...
  16. pmc Circular dichroism and UV resonance raman study of the impact of alcohols on the Gibbs free energy landscape of an alpha-helical peptide
    Kan Xiong
    Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    Biochemistry 49:3336-42. 2010
    ..Turns are also stabilized by alcohols. We also found that while TFE induces more alpha-helices, it favors multiple, shorter helix segments...
  17. ncbi request reprint Direct UV Raman monitoring of 3(10)-helix and pi-bulge premelting during alpha-helix unfolding
    Aleksandr V Mikhonin
    Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    J Am Chem Soc 128:13789-95. 2006
    ..These results will serve as a benchmark for the numerous untested theoretical studies of protein and peptide folding...
  18. ncbi request reprint UV resonance Raman investigation of a 3(10)-helical peptide reveals a rough energy landscape
    Zeeshan Ahmed
    Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    Biochemistry 45:9068-73. 2006
    ....
  19. ncbi request reprint Peptide secondary structure folding reaction coordinate: correlation between uv raman amide III frequency, Psi Ramachandran angle, and hydrogen bonding
    Aleksandr V Mikhonin
    Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    J Phys Chem B 110:1928-43. 2006
    ..As shown elsewhere (Mikhonin et al. J. Am. Chem. Soc. 2005, 127, 7712), this correlation can be used to determine portions of the energy landscape along the Psi reaction coordinate...
  20. ncbi request reprint UV Raman spatially resolved melting dynamics of isotopically labeled polyalanyl peptide: slow alpha-helix melting follows 3(10)-helices and pi-bulges premelting
    Aleksandr V Mikhonin
    Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    J Phys Chem B 111:3280-92. 2007
    ....
  21. ncbi request reprint UV raman examination of alpha-helical peptide water hydrogen bonding
    Konstantin V Pimenov
    Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260
    J Am Chem Soc 127:2840-1. 2005
    ..The UV Raman amide band frequency shifts can be used to monitor alpha-helix hydrogen bonding...
  22. pmc UV resonance raman investigation of electronic transitions in alpha-helical and polyproline II-like conformations
    Bhavya Sharma
    Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    J Phys Chem B 112:11762-9. 2008
    ..The PPII-like helix appears to also undergo excitonic splitting of its pi-->pi* transition which leads to a 207 nm feature...
  23. ncbi request reprint Modeling of stimulated hydrogel volume changes in photonic crystal Pb2+ sensing materials
    Alexander V Goponenko
    Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    J Am Chem Soc 127:10753-9. 2005
    ..These PCCA materials enable stimulated hydrogel volume transitions to be studied...
  24. ncbi request reprint UV resonance Raman determination of polyproline II, extended 2.5(1)-helix, and beta-sheet Psi angle energy landscape in poly-L-lysine and poly-L-glutamic acid
    Aleksandr V Mikhonin
    Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    J Am Chem Soc 127:7712-20. 2005
    ..We believe that these are the first experimental studies to give direct information on protein and peptide energy landscapes...
  25. ncbi request reprint Photonic crystal glucose-sensing material for noninvasive monitoring of glucose in tear fluid
    Vladimir L Alexeev
    Department of Chemistry, Chevron Science Center, Department of Pediatrics, University of Pittsburgh Medical School, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Clin Chem 50:2353-60. 2004
    ..The aim of the present work was to improve this approach for application to noninvasive or minimally invasive monitoring of glucose...
  26. pmc UV resonance Raman determination of molecular mechanism of poly(N-isopropylacrylamide) volume phase transition
    Zeeshan Ahmed
    Department of Chemistry, University of Pittsburgh, Pennsylvania 15260, USA
    J Phys Chem B 113:4248-56. 2009
    ..A persistent length of n approximately 10 suggests a cooperative collapse where hydrophobic interactions between adjacent hydrophobic pockets stabilize the collapsed PNIPAM...
  27. pmc Dependence of the AmII'p proline Raman band on peptide conformation
    Zeeshan Ahmed
    Department of Chemistry, University of Pittsburgh, Pennsylvania 15260, USA
    J Phys Chem B 113:11252-9. 2009
    ....
  28. ncbi request reprint UV resonance Raman measurements of poly-L-lysine's conformational energy landscapes: dependence on perchlorate concentration and temperature
    Lu Ma
    Department of Chemistry, University of Pittsburgh, Pittsburgh, PA 15260, USA
    J Phys Chem B 111:7675-80. 2007
    ..We observe a basin in the Psi angle conformational space associated with alpha-helix and pi-bulge/helix conformations and another basin for the extended PPII and 2.51-helical conformations...
  29. pmc Sodium perchlorate effects on the helical stability of a mainly alanine peptide
    Eliana K Asciutto
    Department of Chemistry and Biochemistry, Center for Computational Sciences, Duquesne University, Pittsburgh, Pennsylvania, USA
    Biophys J 98:186-96. 2010
    ..A direct mechanism was found in which ClO(4)(-) ions are strongly attracted to the folded backbone...
  30. ncbi request reprint Acetylcholinesterase-based organophosphate nerve agent sensing photonic crystal
    Jeremy P Walker
    Department of Chemistry, Chevron Science Center, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    Anal Chem 77:1596-600. 2005
    ..The magnitude of the diffraction red-shift is proportional to the amount of bound parathion. These AChE-PCCAs act as dosimeters for parathion since it irreversibly binds. Parathion concentrations as low as 4.26 fM are easily detected...
  31. ncbi request reprint Synthesis and utilization of monodisperse hollow polymeric particles in photonic crystals
    Xiangling Xu
    Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    J Am Chem Soc 126:7940-5. 2004
    ..For example, we were able to fabricate monodisperse polymer spheres containing high concentrations of magnetic nanospheres formed by direct precipitation within the hollow cores...
  32. ncbi request reprint Electrochemical investigation of Pb2+ binding and transport through a polymerized crystalline colloidal array hydrogel containing benzo-18-crown-6
    Caroline D Geary
    Department of Chemistry, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Anal Chem 77:185-92. 2005
    ..This is attributed to heterogeneities in the crown concentration in the gel. Analysis of diffusion coefficients and rate constants show that diffusion and not chemical relaxation will limit the time response of the material...
  33. pmc UV resonance Raman studies of the NaClO4 dependence of poly-L-lysine conformation and hydrogen exchange kinetics
    Lu Ma
    Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    J Phys Chem B 116:1134-42. 2012
    ..In addition, ClO(4)(-) binding also slows the conformational exchange between the extended and α-helix-like conformations, probably by increasing the activation barriers for conformational interchanges...
  34. ncbi request reprint UV-resonance raman thermal unfolding study of Trp-cage shows that it is not a simple two-state miniprotein
    Zeeshan Ahmed
    Department of Chemistry, University of Pittsburgh, PA 15260, USA
    J Am Chem Soc 127:10943-50. 2005
    ..Possibly, the previous kinetic results monitor only a small region of the Trp-cage which locally appears two-state. This would then argue for spatially decoupled folding even for this small peptide...
  35. pmc Charge stabilized crystalline colloidal arrays as templates for fabrication of non-close-packed inverted photonic crystals
    Justin J Bohn
    Department of Chemistry, University of Pittsburgh, Pittsburgh, PA 15260, United States
    J Colloid Interface Sci 344:298-307. 2010
    ..We show that colloidal particle size and charge polydispersity has modest impact on ordering, in contrast to that for close-packed crystals...
  36. ncbi request reprint Comparison between UV Raman and circular dichroism detection of short alpha helices in bombolitin III
    Abdil Ozdemir
    Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260
    Biochemistry 41:1893-6. 2002
    ..This study demonstrates that UV Raman measurements can detect the formation of single alpha-helical turns which cannot be detected by CD measurements...
  37. pmc UV resonance Raman investigation of the conformations and lowest energy allowed electronic excited states of tri- and tetraalanine: charge transfer transitions
    Bhavya Sharma
    Department of Chemistry, University of Pittsburgh, 219 Parkman Avenue, Pittsburgh, Pennsylvania 15260, USA
    J Phys Chem B 114:6661-8. 2010
    ..Raman depolarization ratio measurements support this assignment. An additional electronic transition is found in Ala(4) at 206 nm...
  38. ncbi request reprint A general photonic crystal sensing motif: creatinine in bodily fluids
    Anjal C Sharma
    Department of Chemistry, University of Pittsburgh, Pennsylvania 15260, USA
    J Am Chem Soc 126:2971-7. 2004
    ..This sensing technology platform is quite general. It may be used to fabricate photonic crystal sensors for any species for which there exists an enzyme which catalyzes it to release H(+) or OH(-)...
  39. doi request reprint Circular dichroism and UV-resonance Raman investigation of the temperature dependence of the conformations of linear and cyclic elastin
    Zeeshan Ahmed
    Department of Chemistry, University of Pittsburgh, PA 15260, USA
    Biopolymers 91:52-60. 2009
    ..Increasing temperature to 60 degrees C results in a small increase in the turn population...
  40. ncbi request reprint Progress toward the development of a point-of-care photonic crystal ammonia sensor
    Kyle W Kimble
    Department of Chemistry, School of Medicine, Chevron Science Center, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Anal Bioanal Chem 385:678-85. 2006
    ..We demonstrate that the NH3 photonic crystal sensing material is capable of quantitative determination of concentrations in the physiological range (50-350 micromol NH3 L(-1)) in human blood serum...
  41. pmc Colloidal crystal growth monitored by Bragg diffraction interference fringes
    Justin J Bohn
    Department of Chemistry, University of Pittsburgh, Pittsburgh, PA 15260, USA
    J Colloid Interface Sci 350:381-6. 2010
    ..The final thickness of wall-nucleated CCA, that is determined by the competition between growth of heterogeneously and homogenously nucleated CCA, increases with higher NaCl concentrations...
  42. ncbi request reprint Peptide bond vibrational coupling
    Nataliya S Myshakina
    Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    J Phys Chem B 111:4271-9. 2007
    ..Our calculated results in general agree with our previous experimental UV Raman studies of a 21-residue mainly alanine-based peptide (AP)...
  43. ncbi request reprint UV resonance raman spectroscopic detection of nitrate and nitrite in wastewater treatment processes
    Anatoli Lanoul
    Department of Chemistry, University of Pittsburgh, Pennsylvania 15260, USA
    Anal Chem 74:1458-61. 2002
    ..UV resonance Raman spectroscopy appears to be an excellent tool for on-line monitoring of NO2-/NO3- in wastewater for the real-time control of water treatment plants...
  44. doi request reprint Polymerized PolyHEMA photonic crystals: pH and ethanol sensor materials
    Xiangling Xu
    Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    J Am Chem Soc 130:3113-9. 2008
    ..The kinetics of equilibration is very slow due to the ultralow diffusion constant of protons in the carboxylated PCCA as predicted earlier by the Tanaka group...
  45. doi request reprint Progress in developing polymerized crystalline colloidal array sensors for point-of-care detection of myocardial ischemia
    Justin T Baca
    Department of Chemistry, Univeristy of Pittsburgh, 219 Parkman Ave, Pittsburgh, PA 15260, USA
    Analyst 133:385-90. 2008
    ..PCCA sensors offer a practical approach to rapid, point-of-care detection of a proposed biochemical signature of myocardial ischemia...
  46. pmc Dependence of glycine CH2 stretching frequencies on conformation, ionization state, and hydrogen bonding
    Sergei V Bykov
    Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    J Phys Chem B 112:5803-12. 2008
    ..According to our experimental observations and theoretical calculations the temperature dependence of the nusCH2 and nuasCH2 of gly is due to the change in the hydrogen-bonding strength of the amine/carboxyl groups to water...
  47. pmc Dependence of amide vibrations on hydrogen bonding
    Nataliya S Myshakina
    Department of Chemistry, University of Pittsburgh, Pennsylvania
    J Phys Chem B 112:11873-7. 2008
    ..Our work shows that a clear understanding of the normal mode composition of the amide vibrations is crucial for an accurate interpretation of the hydrogen bonding dependence of amide vibrational frequencies...
  48. ncbi request reprint Steady-state and transient ultraviolet resonance Raman spectrometer for the 193-270 nm spectral region
    Sergei Bykov
    Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    Appl Spectrosc 59:1541-52. 2005
    ..For the T-jump measurements we use a second camera to simultaneously acquire the Raman spectra of the water stretching bands (2500-4000 cm(-1)) whose band-shape and frequency report the sample temperature...
  49. ncbi request reprint Mass spectral determination of fasting tear glucose concentrations in nondiabetic volunteers
    Justin T Baca
    Department of Chemistry, University of Pittsburgh, Pittsburgh, PA, USA
    Clin Chem 53:1370-2. 2007
    ..Improved sampling and analysis methods may resolve these discrepancies and possibly provide a basis for in situ tear glucose sensors...
  50. pmc Analysis of tear glucose concentration with electrospray ionization mass spectrometry
    Christopher R Taormina
    Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    J Am Soc Mass Spectrom 18:332-6. 2007
    ..We found the tear glucose concentration of one fasting nondiabetic subject to be 13 to 51 microM while the onion-induced tear glucose concentration of a different nondiabetic subject to be 211 to 256 microM...
  51. ncbi request reprint Simple nanosecond to minutes transient absorption spectrophotometer
    Aleksandr V Mikhonin
    Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    Appl Spectrosc 59:1534-40. 2005
    ..9 microm laser pulses. In addition, we measured the rate of optical switching induced by a 3 ns 355 nm pump pulse in PCCA functionalized with azobenzene...
  52. ncbi request reprint Tear glucose analysis for the noninvasive detection and monitoring of diabetes mellitus
    Justin T Baca
    Department of Chemistry, Chevron Science Center, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    Ocul Surf 5:280-93. 2007
    ..On the basis of these results, we discuss the future of in vivo tear glucose sensing and outline the studies needed to resolve the remaining questions about the relationship between tear and blood glucose concentrations...
  53. pmc Salt dependence of an alpha-helical peptide folding energy landscapes
    Kan Xiong
    Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    Biochemistry 48:10818-26. 2009
    ..Cl(-) is expected to have a smaller binding affinity and thus stabilizes alpha-helical conformations intermediately between NaClO(4) and Na(2)SO(4). Electrostatic screening stabilizes pi-bulge conformations...
  54. doi request reprint Computational and experimental determination of the alpha-helix unfolding reaction coordinate
    Eliana K Asciutto
    Department of Chemistry and Biochemistry, Center for Computational, Sciences, Duquesne University, Pittsburgh, Pennsylvania 15282, USA
    Biochemistry 47:2046-50. 2008
    ..3 kcal/mol activation barriers between the alpha-helix-like and PPII-like basins. We also find that the alpha-helix-like conformations are quite defective and the alpha-helix-like structure dynamically samples 310-helix and pi-bulges...
  55. ncbi request reprint UVRR spectroscopic studies of valinomycin complex formation in different solvents
    Abdil Ozdemir
    Department of Chemistry, Faculty of Arts and Sciences, Sakarya University, 54100 Serdivan, Sakarya, Turkey
    Spectrochim Acta A Mol Biomol Spectrosc 61:19-26. 2005
    ..We also showed the effects of the solvent polarity on the solution conformation of VM...

Research Grants45

  1. Ultrahigh Throughput Deep UV Raman Spectrometer for Probing Protein Structure
    Sanford A Asher; Fiscal Year: 2010
    ..The resulting more powerful UV Raman instrumentation will give new insights into the mechanism(s) of protein folding, which is arguably the most important outstanding problem in enzymology. ..
  2. UV RESONANCE RAMAN STUDIES OF PROTEIN STRUCTURE
    Sanford Asher; Fiscal Year: 2002
    ..We will continue to advance the enabling UV Raman instrumentation with the objective of increasing sensitivity, selectivity and information content. ..
  3. NOVEL CLINICAL CHEMISTRY SENSING MOTIF
    Sanford Asher; Fiscal Year: 2001
    ..This motif is highly flexible and can be modified to sense a broad variety and concentration of biologically relevant analytes. ..
  4. Development of (Non) Invasive Real-Time Glucose Sensors
    Sanford Asher; Fiscal Year: 2005
    ..abstract_text> ..
  5. NOVEL CLINICAL CHEMISTRY SENSING MOTIF
    Sanford Asher; Fiscal Year: 2009
    ..The analyte sensing will be monitored by using a simple Palm Pilot-type instrument that utilizes a color CCD camera. The results could be immediately displayed, and easily recorded if the device is operated as a patient biographer. ..
  6. UV RESONANCE RAMAN STUDIES OF PROTEIN STRUCTURE
    Sanford Asher; Fiscal Year: 2007
    ....
  7. NOVEL CLINICAL CHEMISTRY SENSING MOTIF
    Sanford Asher; Fiscal Year: 2007
    ..The analyte sensing will be monitored by using a simple Palm Pilot-type instrument that utilizes a color CCD camera. The results could be immediately displayed, and easily recorded if the device is operated as a patient biographer. ..
  8. UV RESONANCE RAMAN STUDIES OF PROTEIN STRUCTURE
    Sanford Asher; Fiscal Year: 2001
    ..We will improve UV Raman instrumentation, to increase its sensitivity. ..
  9. DEVELOPMENT OF (NON) INVASIVE REAL TIME GLUCOSE SENSORS
    Sanford Asher; Fiscal Year: 1999
    ..This work will advance this sensing technology to the point it can be further developed as an inexpensive, simple, continuous glucose sensor for use in humans. ..
  10. UV RESONANCE RAMAN STUDIES OF PROTEIN STRUCTURE
    Sanford Asher; Fiscal Year: 1992
    ..We will attempt, for the first time, to use vibrational circular dichroism to study interactions between the distal heme residues and heme bound ligands such as azide...