Kristi Anseth

Summary

Affiliation: University of Colorado
Country: USA

Publications

  1. pmc Glucagon-like peptide-1 functionalized PEG hydrogels promote survival and function of encapsulated pancreatic beta-cells
    Chien Chi Lin
    Department of Chemical and Biological Engineering and Howard Hughes Medical Institute, University of Colorado, 424 UCB, Boulder, Colorado 80309, USA
    Biomacromolecules 10:2460-7. 2009
  2. pmc Synthetically tractable click hydrogels for three-dimensional cell culture formed using tetrazine-norbornene chemistry
    Daniel L Alge
    Department of Chemical and Biological Engineering, the BioFrontiers Institute, and the Howard Hughes Medical Institute, University of Colorado at Boulder, Boulder, Colorado 80309, USA
    Biomacromolecules 14:949-53. 2013
  3. pmc Human neutrophil elastase responsive delivery from poly(ethylene glycol) hydrogels
    Alex A Aimetti
    Department of Chemical and Biological Engineering, Howard Hughes Medical Institute, University of Colorado, Boulder, Colorado 80309, USA
    Biomacromolecules 10:1484-9. 2009
  4. pmc Characterization of cell subpopulations expressing progenitor cell markers in porcine cardiac valves
    Huan Wang
    Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, Colorado, United States of America
    PLoS ONE 8:e69667. 2013
  5. pmc Sequential click reactions for synthesizing and patterning three-dimensional cell microenvironments
    Cole A DeForest
    Department of Chemical and Biological Engineering, University of Colorado, UCB Box 424 Boulder, Colorado 80309 0424, USA
    Nat Mater 8:659-64. 2009
  6. pmc Affinity peptides protect transforming growth factor beta during encapsulation in poly(ethylene glycol) hydrogels
    Joshua D McCall
    Department of Chemical and Biological Engineering, University of Colorado, Boulder, Colorado 80309 0424, United States
    Biomacromolecules 12:1051-7. 2011
  7. pmc Manipulation of miRNA activity accelerates osteogenic differentiation of hMSCs in engineered 3D scaffolds
    Peter D Mariner
    Howard Hughes Medical Institute, University of Colorado at Boulder, CO, USA
    J Tissue Eng Regen Med 6:314-24. 2012
  8. pmc Cytocompatible click-based hydrogels with dynamically tunable properties through orthogonal photoconjugation and photocleavage reactions
    Cole A DeForest
    Department of Chemical and Biological Engineering, University of Colorado, UCB Box 424, Boulder, Colorado 80309 0424, USA
    Nat Chem 3:925-31. 2011
  9. pmc Thiol-ene photopolymerizations provide a facile method to encapsulate proteins and maintain their bioactivity
    Joshua D McCall
    Department of Chemical and Biological Engineering and the BioFrontiers Institute, University of Colorado at Boulder, Boulder, Colorado 80303, United States
    Biomacromolecules 13:2410-7. 2012
  10. pmc In situ control of cell substrate microtopographies using photolabile hydrogels
    Chelsea M Kirschner
    Department of Chemical and Biological Engineering, University of Colorado, 596 UCB, Boulder, Colorado, 80303 1904, USA
    Small 9:578-84. 2013

Collaborators

Detail Information

Publications97

  1. pmc Glucagon-like peptide-1 functionalized PEG hydrogels promote survival and function of encapsulated pancreatic beta-cells
    Chien Chi Lin
    Department of Chemical and Biological Engineering and Howard Hughes Medical Institute, University of Colorado, 424 UCB, Boulder, Colorado 80309, USA
    Biomacromolecules 10:2460-7. 2009
    ..Overall, this study demonstrates a strategy to modify PEG hydrogels with bioactive peptide moieties that can significantly enhance the efficacy of islet encapsulation...
  2. pmc Synthetically tractable click hydrogels for three-dimensional cell culture formed using tetrazine-norbornene chemistry
    Daniel L Alge
    Department of Chemical and Biological Engineering, the BioFrontiers Institute, and the Howard Hughes Medical Institute, University of Colorado at Boulder, Boulder, Colorado 80309, USA
    Biomacromolecules 14:949-53. 2013
    ....
  3. pmc Human neutrophil elastase responsive delivery from poly(ethylene glycol) hydrogels
    Alex A Aimetti
    Department of Chemical and Biological Engineering, Howard Hughes Medical Institute, University of Colorado, Boulder, Colorado 80309, USA
    Biomacromolecules 10:1484-9. 2009
    ..The hydrogel platform presented shows highly controlled release kinetics with potential applications in cellular responsive drug delivery...
  4. pmc Characterization of cell subpopulations expressing progenitor cell markers in porcine cardiac valves
    Huan Wang
    Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, Colorado, United States of America
    PLoS ONE 8:e69667. 2013
    ....
  5. pmc Sequential click reactions for synthesizing and patterning three-dimensional cell microenvironments
    Cole A DeForest
    Department of Chemical and Biological Engineering, University of Colorado, UCB Box 424 Boulder, Colorado 80309 0424, USA
    Nat Mater 8:659-64. 2009
    ..This synthetic approach uniquely allows for the direct fabrication of biologically functionalized gels with ideal structures that can be photopatterned, and all in the presence of cells...
  6. pmc Affinity peptides protect transforming growth factor beta during encapsulation in poly(ethylene glycol) hydrogels
    Joshua D McCall
    Department of Chemical and Biological Engineering, University of Colorado, Boulder, Colorado 80309 0424, United States
    Biomacromolecules 12:1051-7. 2011
    ..9-fold, compared to hydrogels with no peptide. Further, increasing the concentration of affinity peptides by a factor of 100-10000 relative to the TGFβ(1) concentration increased fractional recovery of the protein from PEG hydrogels...
  7. pmc Manipulation of miRNA activity accelerates osteogenic differentiation of hMSCs in engineered 3D scaffolds
    Peter D Mariner
    Howard Hughes Medical Institute, University of Colorado at Boulder, CO, USA
    J Tissue Eng Regen Med 6:314-24. 2012
    ....
  8. pmc Cytocompatible click-based hydrogels with dynamically tunable properties through orthogonal photoconjugation and photocleavage reactions
    Cole A DeForest
    Department of Chemical and Biological Engineering, University of Colorado, UCB Box 424, Boulder, Colorado 80309 0424, USA
    Nat Chem 3:925-31. 2011
    ..The results demonstrate the versatile nature of the chemistry to create programmable niches to study and direct cell function by modifying the local hydrogel environment...
  9. pmc Thiol-ene photopolymerizations provide a facile method to encapsulate proteins and maintain their bioactivity
    Joshua D McCall
    Department of Chemical and Biological Engineering and the BioFrontiers Institute, University of Colorado at Boulder, Boulder, Colorado 80303, United States
    Biomacromolecules 13:2410-7. 2012
    ..When the cytokine TGFβ was encapsulated in PEG hydrogels formed via the thiol-ene reaction, full protein bioactivity was preserved...
  10. pmc In situ control of cell substrate microtopographies using photolabile hydrogels
    Chelsea M Kirschner
    Department of Chemical and Biological Engineering, University of Colorado, 596 UCB, Boulder, Colorado, 80303 1904, USA
    Small 9:578-84. 2013
    ....
  11. pmc Redirecting valvular myofibroblasts into dormant fibroblasts through light-mediated reduction in substrate modulus
    Huan Wang
    Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, Colorado, United States of America
    PLoS ONE 7:e39969. 2012
    ..These results provide insight in designing hydrogel substrates with physiologically relevant stiffness to dynamically redirect cell fate in vitro...
  12. pmc Photocontrolled nanoparticles for on-demand release of proteins
    Malar A Azagarsamy
    Department of Chemical and Biological Engineering and the Howard Hughes Medical Institute, University of Colorado at Boulder, Boulder, Colorado 80303, United States
    Biomacromolecules 13:2219-24. 2012
    ....
  13. pmc Responsive culture platform to examine the influence of microenvironmental geometry on cell function in 3D
    April M Kloxin
    Chemical and Biological Engineering, University of Colorado, Boulder, CO, USA
    Integr Biol (Camb) 4:1540-9. 2012
    ..Collectively, these studies demonstrate the development and utility of responsive hydrogel culture devices to study how a range of microenvironment geometries of evolving shape and connectivity might influence or direct cell function...
  14. pmc Inhibition of Staphylococcus epidermidis biofilms using polymerizable vancomycin derivatives
    McKinley C Lawson
    Department of Chemical and Biological Engineering, University of Colorado, Boulder, CO, USA
    Clin Orthop Relat Res 468:2081-91. 2010
    ..In orthopaedic surgery, this problem is exacerbated by the large number and variety of material types that are implanted. Metallic hardware in conjunction with polymethylmethacrylate (PMMA) bone cement is commonly used...
  15. ncbi request reprint A synthetic strategy for mimicking the extracellular matrix provides new insight about tumor cell migration
    Michael P Schwartz
    Department of Chemical and Biological Engineering, Howard Hughes Medical Institute, University of Colorado at Boulder, ECCH111, CB424, Boulder, CO 80309, USA
    Integr Biol (Camb) 2:32-40. 2010
    ..The migration mode observed here provides insight into the invasiveness of metastatic cells in vivo and demonstrates the potential of a synthetic strategy for investigating complex biological problems...
  16. ncbi request reprint In situ forming degradable networks and their application in tissue engineering and drug delivery
    Kristi S Anseth
    Engineering Center, Room ECCH 111, Department of Chemical Engineering, University of Colorado, Boulder, CO 80309 0424, USA
    J Control Release 78:199-209. 2002
    ..Finally, the application of these macromers as in situ forming hydrogel constructs for cartilage tissue engineering is demonstrated...
  17. pmc Ultrasound monitoring of cartilaginous matrix evolution in degradable PEG hydrogels
    Mark A Rice
    Department of Chemical and Biological Engineering, University of Colorado, Boulder, CO 80309 0424, USA
    Acta Biomater 5:152-61. 2009
    ..Rigorous studies of the relationships identified in this study will lead to non-destructive real-time monitoring that will be useful in combination with bioreactors used to promote and study cartilage regeneration...
  18. ncbi request reprint Synthesis and characterization of a fluvastatin-releasing hydrogel delivery system to modulate hMSC differentiation and function for bone regeneration
    Danielle S W Benoit
    Department of Chemical and Biological Engineering, University of Colorado, Boulder, CO 80309 0424, USA
    Biomaterials 27:6102-10. 2006
    ..Therefore, fluvastatin-releasing hydrogels may be useful in bone tissue engineering applications, not only for triggering osteogenic differentiation of hMSCs, but also by modulating their function...
  19. ncbi request reprint Temporal changes in peg hydrogel structure influence human mesenchymal stem cell proliferation and matrix mineralization
    Charles R Nuttelman
    Department of Chemical and Biological Engineering, University of Colorado, Boulder, Colorado, USA
    Adv Exp Med Biol 585:135-49. 2006
  20. ncbi request reprint Decorin moieties tethered into PEG networks induce chondrogenesis of human mesenchymal stem cells
    Chelsea N Salinas
    Department of Chemical and Biological Engineering, University of Colorado, Boulder, Colorado, USA
    J Biomed Mater Res A 90:456-64. 2009
    ..Collectively, these results demonstrate how incorporation of matrix-binding peptide interacts with hMSCs inducing chondrogenic differentiation and cartilage-specific ECM deposition...
  21. pmc Photocrosslinking of gelatin macromers to synthesize porous hydrogels that promote valvular interstitial cell function
    Julie A Benton
    Department of Chemical and Biological Engineering, University of Colorado, Boulder, Colorado 80309 0424, USA
    Tissue Eng Part A 15:3221-30. 2009
    ..Although these studies were limited to VICs, this novel hydrogel system may also be useful for studying other fibroblastic cell types...
  22. ncbi request reprint Serum deprivation improves seeding and repopulation of acellular matrices with valvular interstitial cells
    Melinda C Cushing
    Department of Chemical and Biological Engineering, ECCH 111, UCB 424, University of Colorado, Boulder, CO 80309 0424, USA
    J Biomed Mater Res A 75:232-41. 2005
    ....
  23. ncbi request reprint Manipulations in hydrogel chemistry control photoencapsulated chondrocyte behavior and their extracellular matrix production
    Stephanie J Bryant
    Department of Chemical Engineering, University of Colorado, ECCH 111, Boulder, Colorado 80309 0424, USA
    J Biomed Mater Res A 67:1430-6. 2003
    ..58 +/- 1.21, depending on gel chemistry and in vitro versus in vivo culture environment. By tuning scaffold chemistry, and subsequently, gel structure and degradation behavior, we can better guide tissue evolution and development...
  24. ncbi request reprint Heparin functionalized PEG gels that modulate protein adsorption for hMSC adhesion and differentiation
    Danielle S W Benoit
    Department of Chemical and Biological Engineering, University of Colorado, Boulder, 80309 0424, USA
    Acta Biomater 1:461-70. 2005
    ..Finally, the gels were examined as a potential scaffold for hMSC culture and were found to promote adhesion, proliferation, and osteogenic differentiation...
  25. ncbi request reprint Integrin-linked kinase production prevents anoikis in human mesenchymal stem cells
    Danielle S W Benoit
    Department of Chemical and Biological Engineering, University of Colorado, 424 UCB ECCH 111, Boulder, Colorado 80309, USA
    J Biomed Mater Res A 81:259-68. 2007
    ..In sum, ILK infection can support cell survival in the absence of matrix interactions and enable fundamental studies of three-dimensional cell function in response to extrinsic signals, independently of matrix-ligand interactions...
  26. ncbi request reprint Chondrogenic differentiation potential of human mesenchymal stem cells photoencapsulated within poly(ethylene glycol)-arginine-glycine-aspartic acid-serine thiol-methacrylate mixed-mode networks
    Chelsea N Salinas
    Department of Chemical and Biological Engineering, University of Colorado, Boulder, Colorado 80309, USA
    Tissue Eng 13:1025-34. 2007
    ..Incorporation of this peptide sequence not only allows for sustained viability, but also contributes to initiating chondrogenesis...
  27. ncbi request reprint Controlling the spatial distribution of ECM components in degradable PEG hydrogels for tissue engineering cartilage
    Stephanie J Bryant
    Department of Chemical Engineering, University of Colorado, ECCH 111, Boulder, Colorado 80309 0424, USA
    J Biomed Mater Res A 64:70-9. 2003
    ..In summary, the important role of hydrogel degradation in controlling and influencing the deposition and distribution of extracellular matrix molecules was demonstrated and quantified...
  28. pmc The enhancement of chondrogenic differentiation of human mesenchymal stem cells by enzymatically regulated RGD functionalities
    Chelsea N Salinas
    Department of Chemical and Biological Engineering, University of Colorado, Boulder, CO, United States
    Biomaterials 29:2370-7. 2008
    ..Collectively, these data provide evidence that temporal regulation of integrin-binding peptides is important in the design of niches in differentiating hMSCs to chondrocytes...
  29. ncbi request reprint Photoencapsulation of osteoblasts in injectable RGD-modified PEG hydrogels for bone tissue engineering
    Jason A Burdick
    Department of Chemical Engineering, University of Colorado, Boulder 80309 0424, USA
    Biomaterials 23:4315-23. 2002
    ..Finally, mineral deposits were seen in all hydrogels after 4 weeks of in vitro culture, but a significant increase in mineralization was observed upon introduction of adhesive peptides throughout the network...
  30. ncbi request reprint Crosslinked hyaluronan scaffolds as a biologically active carrier for valvular interstitial cells
    Kristyn S Masters
    Howard Hughes Medical Institute, University of Colorado, Boulder, CO 80309 0424, USA
    Biomaterials 26:2517-25. 2005
    ..This work shows promise for the creation of a tissue-engineered heart valve utilizing the synergistic relationship between hyaluronic acid and VICs...
  31. ncbi request reprint Controlling cartilaginous matrix evolution in hydrogels with degradation triggered by exogenous addition of an enzyme
    Mark A Rice
    Department of Chemical and Biological Engineering, University of Colorado, Boulder, Colorado 80309 0424, USA
    Tissue Eng 13:683-91. 2007
    ..The hydrogels applied in this study allow explicit control over degradation, and therefore provide a useful tool for investigating the effects of specific mass loss profiles on the evolution of neocartilage in vitro...
  32. ncbi request reprint PEG-based hydrogels as an in vitro encapsulation platform for testing controlled beta-cell microenvironments
    Laney M Weber
    Department of Chemical and Biological Engineering, University of Colorado, ECCH 111, UCB 424, Boulder, 80309 0424, USA
    Acta Biomater 2:1-8. 2006
    ..Finally, the compatibility of the PEG encapsulation system with freshly isolated islets was confirmed...
  33. pmc Small functional groups for controlled differentiation of hydrogel-encapsulated human mesenchymal stem cells
    Danielle S W Benoit
    Department of Chemical and Biological Engineering, University of Colorado, 424 UCB ECCH 111, Boulder, Colorado 80309, USA
    Nat Mater 7:816-23. 2008
    ..Strategies using simple chemistry to control complex biological processes would be particularly powerful as they could make production of therapeutic materials simpler, cheaper and more easily controlled...
  34. ncbi request reprint Designing scaffolds for valvular interstitial cells: cell adhesion and function on naturally derived materials
    Kristyn S Masters
    Howard Hughes Medical Institute, Chevy Chase, Maryland, USA
    J Biomed Mater Res A 71:172-80. 2004
    ....
  35. pmc Stop-flow lithography for the production of shape-evolving degradable microgel particles
    Dae Kun Hwang
    Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    J Am Chem Soc 131:4499-504. 2009
    ..At higher UV doses, a saturation in cross-linking density occurs and bulk-degrading behavior is observed. Finally, we synthesized multifunctional composite particles, providing unique features not found in homogeneous hydrogels...
  36. doi request reprint PEG hydrogels for the controlled release of biomolecules in regenerative medicine
    Chien Chi Lin
    Department of Chemical and Biological Engineering, University of Colorado, Boulder, Colorado, 80309, USA
    Pharm Res 26:631-43. 2009
    ....
  37. pmc Photodegradable hydrogels for dynamic tuning of physical and chemical properties
    April M Kloxin
    Department of Chemical and Biological Engineering, University of Colorado, 424 UCB ECCH 111, Boulder, CO 80309, USA
    Science 324:59-63. 2009
    ....
  38. ncbi request reprint Material-based regulation of the myofibroblast phenotype
    Melinda C Cushing
    Department of Chemical and Biological Engineering, University of Colorado, Boulder, CO 80309 0424, USA
    Biomaterials 28:3378-87. 2007
    ..Biomaterial scaffolds targeting cell surface growth factor receptors are a promising new direction for regulating cell functions in tissue-engineering applications...
  39. pmc Functional PEG-peptide hydrogels to modulate local inflammation induced by the pro-inflammatory cytokine TNFalpha
    Chien Chi Lin
    Department of Chemical and Biological Engineering, University of Colorado, 424 UCB, Boulder, CO 80309, USA
    Biomaterials 30:4907-14. 2009
    ..This study demonstrates the importance of controlling the availability of pro-inflammatory cytokines in highly permissive hydrogels...
  40. ncbi request reprint Crosslinking density influences chondrocyte metabolism in dynamically loaded photocrosslinked poly(ethylene glycol) hydrogels
    Stephanie J Bryant
    Department of Chemical Engineering, University of Colorado, Boulder, CO 80309 0424, USA
    Ann Biomed Eng 32:407-17. 2004
    ..In summary, changes in PEG hydrogel properties greatly affect how chondrocytes respond to an applied dynamic load...
  41. ncbi request reprint Encapsulating chondrocytes in degrading PEG hydrogels with high modulus: engineering gel structural changes to facilitate cartilaginous tissue production
    Stephanie J Bryant
    Department of Chemical Engineering, University of Colorado, Boulder, CO 80309 0424, USA
    Biotechnol Bioeng 86:747-55. 2004
    ....
  42. pmc Photoinitiated polymerization of PEG-diacrylate with lithium phenyl-2,4,6-trimethylbenzoylphosphinate: polymerization rate and cytocompatibility
    Benjamin D Fairbanks
    Department of Chemical and Biological Engineering, University of Colorado, Boulder, Colorado 80309 0424, USA
    Biomaterials 30:6702-7. 2009
    ..When examined 24h after encapsulation, survival rates of human neonatal fibroblasts encapsulated in hydrogels polymerized with the phosphinate initiator exceed 95%, demonstrating the cytocompatibility of this initiating system...
  43. ncbi request reprint Synthesis and characterization of photocrosslinkable, degradable poly(vinyl alcohol)-based tissue engineering scaffolds
    Charles R Nuttelman
    Department of Chemical Engineering, University of Colorado at Boulder, Engineering Center, 80309 0424, USA
    Biomaterials 23:3617-26. 2002
    ..In addition, by increasing the network's hydrophobicity, valve interstitial cell adhesion is improved...
  44. ncbi request reprint Controlled polymerization chemistry to graft architectures that influence cell-material interactions
    Robert P Sebra
    Department of Chemical and Biological Engineering, ECCH 111, CB424, University of Colorado, Boulder, CO 80309 0424, United States
    Acta Biomater 3:151-61. 2007
    ..Collectively, the results herein show the utility and application of a spatially and temporally controlled photografting process for designing cell responsive polymer surfaces...
  45. ncbi request reprint Dexamethasone-functionalized gels induce osteogenic differentiation of encapsulated hMSCs
    Charles R Nuttelman
    Department of Chemical and Biological Engineering, University of Colorado, Boulder, Colorado 80309 0424, USA
    J Biomed Mater Res A 76:183-95. 2006
    ..These studies indicate that encapsulated hMSCs are capable of osteogenic differentiation in response to released dexamethasone...
  46. ncbi request reprint Contrasting effects of collagen and bFGF-2 on neural cell function in degradable synthetic PEG hydrogels
    Melissa J Mahoney
    Department of Chemical and Biological Engineering, University of Colorado, Campus Box 424, Boulder, Colorado 80309, USA
    J Biomed Mater Res A 81:269-78. 2007
    ..These findings suggest the importance of understanding and developing strategies to control the chemical microenvironment surrounding cells in three-dimensional biomaterials...
  47. ncbi request reprint Three-dimensional growth and function of neural tissue in degradable polyethylene glycol hydrogels
    Melissa J Mahoney
    Department of Chemical and Biological Engineering and the Howard Hughes Medical Institute, University of Colorado, Boulder, 80309, USA
    Biomaterials 27:2265-74. 2006
    ..These studies were carried out in the absence of serum and extracellular matrix molecules that can be immunogenic and identify degradable PEG hydrogels as suitable synthetic cell carriers for neural transplantation...
  48. pmc Cell-matrix interactions improve beta-cell survival and insulin secretion in three-dimensional culture
    Laney M Weber
    Department of Chemical and Biological Engineering, University of Colorado, Boulder, Colorado 80309, USA
    Tissue Eng Part A 14:1959-68. 2008
    ..These results demonstrate that specific, rationally designed extracellular environments promote isolated beta-cell survival and function...
  49. pmc Repair of a calvarial defect with biofactor and stem cell-embedded polyethylene glycol scaffold
    Adam Terella
    Department of Otolaryngology Head and Neck Surgery, University of Colorado, 12631 E 17th Ave, Aurora, CO 80045, USA
    Arch Facial Plast Surg 12:166-71. 2010
    ....
  50. ncbi request reprint The effects of cell-matrix interactions on encapsulated beta-cell function within hydrogels functionalized with matrix-derived adhesive peptides
    Laney M Weber
    Department of Chemical and Biological Engineering, University of Colorado, Boulder, CO 80309 0424, USA
    Biomaterials 28:3004-11. 2007
    ..The presented results give evidence that synthetic peptide epitopes may be useful in the design of an islet encapsulation environment that promotes cell survival and function via targeted cell-matrix interactions...
  51. pmc Poly(ethylene glycol) hydrogels formed by thiol-ene photopolymerization for enzyme-responsive protein delivery
    Alex A Aimetti
    Department of Chemical and Biological Engineering, University of Colorado, Boulder, CO 80309, USA
    Biomaterials 30:6048-54. 2009
    ..Also, protein release was predicted from a 3-layered hydrogel device using mass loss data. Lastly, the bioactivity of lysozyme was maintained above 90% following the exposure to thiol-ene photopolymerization conditions...
  52. ncbi request reprint Activation of valvular interstitial cells is mediated by transforming growth factor-beta1 interactions with matrix molecules
    Melinda C Cushing
    Department of Chemical and Biological Engineering, University of Colorado, ECCH 111, UCB 424, Boulder, CO 80309 0424, United States
    Matrix Biol 24:428-37. 2005
    ..The characterization of molecules, both soluble and insoluble, that control VIC activation will be important for the development of tailored 3D culture environments for tissue-engineering applications...
  53. ncbi request reprint Hydrogel properties influence ECM production by chondrocytes photoencapsulated in poly(ethylene glycol) hydrogels
    Stephanie J Bryant
    Department of Chemical Engineering, University of Colorado, Boulder, Colorado 80309 0424, USA
    J Biomed Mater Res 59:63-72. 2002
    ..9) were obtained, which allowed for increased type II collagen synthesis coupled with a homogenous distribution of GAGs. Thus, a critical balance exists between gel swelling, mechanics, and degradation in forming a functional ECM...
  54. ncbi request reprint In vitro osteogenic differentiation of human mesenchymal stem cells photoencapsulated in PEG hydrogels
    Charles R Nuttelman
    Department of Chemical and Biological Engineering, University of Colorado, Boulder, CO 80309 0424, USA
    J Biomed Mater Res A 68:773-82. 2004
    ..These experiments demonstrate the feasibility of using a PEG-based, photocrosslinkable system to culture and deliver human mesenchymal stem cells for bone tissue regeneration and repair...
  55. ncbi request reprint Tailoring the degradation of hydrogels formed from multivinyl poly(ethylene glycol) and poly(vinyl alcohol) macromers for cartilage tissue engineering
    Penny J Martens
    Department of Chemical Engineering, University of Colorado, Boulder 80309, USA
    Biomacromolecules 4:283-92. 2003
    ..In summary, fabricating hydrogels through the copolymerization of PEG and PVA macromers is an effective tool for encapsulating chondrocytes, controlling gel degradation profiles, and generating cartilaginous tissue...
  56. ncbi request reprint Multifunctional pancreatic islet encapsulation barriers achieved via multilayer PEG hydrogels
    Laney M Weber
    Department of Chemical and Biological Engineering, University of Colorado, Boulder, CO 80309 0424, USA
    Cell Transplant 16:1049-57. 2008
    ....
  57. ncbi request reprint Controlling network structure in degradable thiol-acrylate biomaterials to tune mass loss behavior
    Amber E Rydholm
    Department of Chemical and Biological Engineering, University of Colorado, Boulder, Colorado 80309 0424, USA
    Biomacromolecules 7:2827-36. 2006
    ..Similarly, decreasing the thiol functionality from 4 to 1 shifted the reverse gelation time from 18 to 8 days and the mass loss extent at reverse gelation from 70 to 45%...
  58. ncbi request reprint The effect of heparin-functionalized PEG hydrogels on three-dimensional human mesenchymal stem cell osteogenic differentiation
    Danielle S W Benoit
    Department of Chemical and Biological Engineering, University of Colorado, Boulder, CO 80309 0424, USA
    Biomaterials 28:66-77. 2007
    ....
  59. pmc Regulating MCP-1 diffusion in affinity hydrogels for enhancing immuno-isolation
    Chien Chi Lin
    Department of Chemical and Biological Engineering, University of Colorado, 424 UCB, Boulder, CO 80309, USA
    J Control Release 142:384-91. 2010
    ....
  60. pmc Using living radical polymerization to enable facile incorporation of materials in microfluidic cell culture devices
    Helen M Simms
    Department of Chemical and Biological Engineering, ECCH 111, CB424, University of Colorado, Boulder, CO 80309 0424, USA
    Biomaterials 29:2228-36. 2008
    ....
  61. ncbi request reprint Delivery of osteoinductive growth factors from degradable PEG hydrogels influences osteoblast differentiation and mineralization
    Jason A Burdick
    Department of Chemical Engineering, University of Colorado, Boulder, CO 80309 0424, USA
    J Control Release 83:53-63. 2002
    ..Finally, ectopic bone formation was present in subcutaneous rat tissue after implantation of hydrogel networks loaded with osteoinductive growth factors...
  62. ncbi request reprint Valvular myofibroblast activation by transforming growth factor-beta: implications for pathological extracellular matrix remodeling in heart valve disease
    Gennyne A Walker
    Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309, USA
    Circ Res 95:253-60. 2004
    ..We suggest that the activation of contractile myofibroblasts by TGF-beta1 may be a significant first step in promoting alterations to the valve matrix architecture that are evident in valvular heart disease...
  63. ncbi request reprint Manipulations in hydrogel degradation behavior enhance osteoblast function and mineralized tissue formation
    Danielle S W Benoit
    Department of Chemical and Biological Engineering, University of Colorado, Boulder, Colorado 80309 0424, USA
    Tissue Eng 12:1663-73. 2006
    ..Mineralization increased with increasing concentration of PEG-LA-DM and biochemically resembled that of hydroxyapatite...
  64. ncbi request reprint Photoinitiated crosslinked degradable copolymer networks for tissue engineering applications
    Kelly A Davis
    Howard Hughes Medical Institute, University of Colorado, Campus Box 424, Boulder, CO 80309, USA
    Biomaterials 24:2485-95. 2003
    ..Cellular compatibility was determined by seeding primary rat calverial osteoblast cells onto the disks and characterizing the cell morphology using scanning electron microscopy...
  65. pmc Hydrogels as extracellular matrix mimics for 3D cell culture
    Mark W Tibbitt
    Department of Chemical and Biological Engineering, University of Colorado, Boulder, CO, USA
    Biotechnol Bioeng 103:655-63. 2009
    ..Ultimately, advances in synthetic-biologic hydrogel hybrids are needed to provide robust platforms for investigating cell physiology and fabricating tissue outside of the organism...
  66. pmc Characterization of valvular interstitial cell function in three dimensional matrix metalloproteinase degradable PEG hydrogels
    Julie A Benton
    Department of Chemical and Biological Engineering, University of Colorado, Boulder, CO 80309 0424, USA
    Biomaterials 30:6593-603. 2009
    ....
  67. ncbi request reprint The effect of ethylene glycol methacrylate phosphate in PEG hydrogels on mineralization and viability of encapsulated hMSCs
    Charles R Nuttelman
    Department of Chemical and Biological Engineering, University of Colorado, Boulder, CO 80309 0424, USA
    Biomaterials 27:1377-86. 2006
    ..It was found that EGMP-containing PEGDA hydrogels are able to sequester the important cell adhesion protein osteopontin, and these hydrogels promoted hMSC adhesion and spreading...
  68. ncbi request reprint Cell-based therapies and tissue engineering
    Mark A Rice
    Department of Chemical and Biological Engineering, University of Colorado, Boulder, CO 80309, USA
    Otolaryngol Clin North Am 38:199-214, v. 2005
    ..Although the application of bioengineered tissue is specific to the anatomic areas of interest,continued advances bring tissue engineering closer to reality in all areas of otolaryngology...
  69. pmc Characterizing multilaminated hydrogels with spatially varying network structure and solute loading using confocal laser scanning microscopy
    Andrew W Watkins
    Department of Chemical and Biological Engineering, University of Colorado, Boulder, CO 80309 0424, USA
    Acta Biomater 3:439-48. 2007
    ..Finally, the simultaneous release of two dyes at different rates from a PEG/PHEMA multilaminate was monitored to demonstrate the usefulness of CLSM in understanding the complex temporal changes in solute distributions in gel devices...
  70. ncbi request reprint Synthetic hydrogel niches that promote hMSC viability
    Charles R Nuttelman
    Department of Chemical and Biological Engineering, University of Colorado, Boulder, CO 80309 0424, USA
    Matrix Biol 24:208-18. 2005
    ..It is believed that the phosphate groups promote mineralization of the hydrogel network, and this mineral phase sequesters cell-secreted osteopontin, resulting in enhanced cell-matrix interactions and improved cell viability...
  71. pmc The effect of bioactive hydrogels on the secretion of extracellular matrix molecules by valvular interstitial cells
    Darshita N Shah
    Department of Chemical and Biological Engineering, University of Colorado, Boulder, CO 80309 0424, USA
    Biomaterials 29:2060-72. 2008
    ..Exogenous HA delivery through the cell culture media further demonstrated the utility of delivered HA on manipulating the secretory properties of encapsulated VICs...
  72. ncbi request reprint Modifying the proliferative state of target cells to control DNA expression and identifying cell types transfected in vivo
    Kathryn W Riddle
    Department of Chemical Engineering, University of Michigan, Ann Arbor, Michigan, USA
    Mol Ther 15:361-8. 2007
    ..The results of these studies suggest that increasing the proliferative state of target cell populations can enhance non-viral gene transfer...
  73. pmc Effects of PEG hydrogel crosslinking density on protein diffusion and encapsulated islet survival and function
    Laney M Weber
    Department of Chemical and Biological Engineering, University of Colorado, Boulder, Colorado 80309 0424, USA
    J Biomed Mater Res A 90:720-9. 2009
    ....
  74. pmc Hydrogel encapsulation environments functionalized with extracellular matrix interactions increase islet insulin secretion
    Laney M Weber
    Department of Chemical and Biological Engineering, Howard Hughes Medical Institute, University of Colorado, Boulder, CO 80309 0424, USA
    Matrix Biol 27:667-73. 2008
    ..Our results demonstrate the potential of specific matrix interactions within an islet encapsulation microenvironment to promote encapsulated islet function...
  75. ncbi request reprint The effect on osteoblast function of colocalized RGD and PHSRN epitopes on PEG surfaces
    Danielle S W Benoit
    Department of Chemical and Biological Engineering, University of Colorado, UCB 424, ECCH 111, Boulder, CO 80309 0424, USA
    Biomaterials 26:5209-20. 2005
    ..This result was attributed to strong receptor-ligand interactions initiating signal transduction cascades that down-regulate ECM production...
  76. ncbi request reprint Crosslinking density influences the morphology of chondrocytes photoencapsulated in PEG hydrogels during the application of compressive strain
    Stephanie J Bryant
    Department of Chemical Engineering, University of Colorado, Campus Box 424, Engineering Center, ECCH 111, Boulder, CO 80309 0424, USA
    J Orthop Res 22:1143-9. 2004
    ..These heterogeneities were greater in the highly crosslinked gel. These findings demonstrate that different levels of cell deformation and heterogeneity may be obtained by varying the crosslinking density in PEG hydrogels...
  77. ncbi request reprint Nanobiomaterial applications in orthopedics
    Elizabeth M Christenson
    Department of Bioengineering MS142, Rice University, PO Box 1892, Houston, Texas 77251 1892, USA
    J Orthop Res 25:11-22. 2007
    ..Overall, current trends in nanobiotechnology foreshadow a bright future through the use of nanobiomaterials in the orthopedic domain...
  78. ncbi request reprint Surface grafted antibodies: controlled architecture permits enhanced antigen detection
    Robert P Sebra
    Langmuir 21:10907-11. 2005
    ..Finally, the addition of PEG spacers between the acrylate functionality and the pendant detection antibodies was tuned to enhance the detection of a short-half-life molecule, glucagon, in a complex biological environment, plasma...
  79. ncbi request reprint Encapsulated chondrocyte response in a pulsatile flow bioreactor
    James A Cooper
    Polymers Division, National Institute of Standards and Technology, 100 Bureau Drive, Gaithersburg, MD 20899 8543, USA
    Acta Biomater 3:13-21. 2007
    ....
  80. ncbi request reprint An investigation of the cytotoxicity and histocompatibility of in situ forming lactic acid based orthopedic biomaterials
    Jason A Burdick
    Department of Chemical Engineering, University of Colorado, Boulder, Colorado 80309, USA
    J Biomed Mater Res 63:484-91. 2002
    ..This finding indicates that an in situ forming porous scaffold of this composition may support the infiltration of surrounding vascularized tissue, and thus be applicable to orthopedic treatments of large bone defects...
  81. ncbi request reprint Application of a kinetic gelation simulation to the characterization of in situ cross-linking biomaterials
    Amy K Poshusta
    Department of Chemical Engineering, University of Colorado, Boulder 80309 0424, USA
    J Biomater Sci Polym Ed 13:797-815. 2002
    ..e. cross-linked polyanhydrides) where appropriate. Simulated results for pendant group reactivity, kinetic chain lengths, and radical concentrations (trapped and free) are presented herein...
  82. pmc Exogenously triggered, enzymatic degradation of photopolymerized hydrogels with polycaprolactone subunits: experimental observation and modeling of mass loss behavior
    Mark A Rice
    Department of Chemical and Biological Engineering, University of Colorado, Boulder, Colorado 80309, USA
    Biomacromolecules 7:1968-75. 2006
    ..The model was used to predict mass loss profiles at the specific conditions used, and also to demonstrate the importance of potential changes in reaction rate and enzyme stability on temporal mass loss...
  83. ncbi request reprint Synthesis of immunoisolation barriers that provide localized immunosuppression for encapsulated pancreatic islets
    Charles Y Cheung
    Department of Chemical and Biological Engineering, Howard Hughes Medical Institute, University of Colorado, Boulder, Colorado 80309 0424, USA
    Bioconjug Chem 17:1036-42. 2006
    ..These experiments promote the concept that surface-conjugated hydrogel constructs can provide localized immunosuppression for encapsulated grafted tissue...
  84. ncbi request reprint Gene delivery in tissue engineering: a photopolymer platform to coencapsulate cells and plasmid DNA
    Deborah J Quick
    Department of Chemical Engineering, University of Colorado, Boulder, CO 80309, USA
    Pharm Res 20:1730-7. 2003
    ..Photopolymerization enables spatial and temporal control of gel formation under physiological conditions, but the presence of photoinitiator radicals poses challenges for DNA photoencapsulation...
  85. pmc Fibroblast growth factor represses Smad-mediated myofibroblast activation in aortic valvular interstitial cells
    Melinda C Cushing
    University of Colorado, Department of Chemical and Biological Engineering, Boulder, CO 80309 0424, USA
    FASEB J 22:1769-77. 2008
    ..Furthermore, FGF-2 treatment of VICs blocks the development of pathological contractile and calcifying phenotypes, suggesting that these pathways may be utilized in the engineering of effective treatments for valvular disease...
  86. doi request reprint Materials science: Protein gels on the move
    April M Kloxin
    Nature 454:705-6. 2008
  87. doi request reprint The influence of the RGD peptide motif and its contextual presentation in PEG gels on human mesenchymal stem cell viability
    Chelsea N Salinas
    Department of Chemical and Biological Engineering, University of Colorado, Boulder, CO 80309 0424, USA
    J Tissue Eng Regen Med 2:296-304. 2008
    ..Integrin staining for alphavbeta3 and alpha4, as well as focal adhesion staining, correlates to the trends in hMSC survival for covalently-bound RGD motifs and a loss of viability for the solubly delivered peptide systems...
  88. doi request reprint Three-dimensional biochemical patterning of click-based composite hydrogels via thiolene photopolymerization
    Brian D Polizzotti
    Department of Chemical and Biological Engineering, Howard Hughes Medical Institute, University of Colorado at Boulder, Boulder, Colorado 80309, USA
    Biomacromolecules 9:1084-7. 2008
    ..Standard photolithographic techniques were used to generate a variety of two- and three-dimensional patterns as well as controlled biochemical gradients within existing preformed hydrogels...
  89. ncbi request reprint Modifying network chemistry in thiol-acrylate photopolymers through postpolymerization functionalization to control cell-material interactions
    Amber E Rydholm
    Department of Chemical and Biological Engineering, University of Colorado, Boulder, Colorado 80309 0424, USA
    J Biomed Mater Res A 86:23-30. 2008
    ..Further, when photocoupling was used to modify the networks, spatial and temporal control of the light exposure facilitated the formation of chemical patterns on the surface and throughout the material...
  90. ncbi request reprint Materials science. Hydrogel cell cultures
    Melinda C Cushing
    Department of Chemical and Biological Engineering, University of Colorado, Boulder, CO 80309, USA
    Science 316:1133-4. 2007
  91. ncbi request reprint Thermomechanics of the shape memory effect in polymers for biomedical applications
    Ken Gall
    Department of Mechanical Engineering, University of Colorado, Boulder, 80309, USA
    J Biomed Mater Res A 73:339-48. 2005
    ..The thermomechanical results are discussed in light of potential biomedical applications, and a prototype device is presented...
  92. ncbi request reprint DNA delivery from photocrosslinked PEG hydrogels: encapsulation efficiency, release profiles, and DNA quality
    Deborah J Quick
    Department of Chemical and Biological Engineering, University of Colorado, Boulder, CO 80309, USA
    J Control Release 96:341-51. 2004
    ....
  93. ncbi request reprint Degradable thiol-acrylate photopolymers: polymerization and degradation behavior of an in situ forming biomaterial
    Amber E Rydholm
    Department of Chemical and Biological Engineering, Engineering Center, University of Colorado, Room ECCH 111, Campus Box 424, Boulder, CO 80309 0424, USA
    Biomaterials 26:4495-506. 2005
    ..Additionally, varying the mole fraction of multifunctional thiol monomer in the initial reaction mixture controls the concentration of reactive sites in the network available for post-polymerization modification of the polymer...

Research Grants27

  1. Online Monitoring and Control of Functional Cartilage Regeneration in Hydrogels
    Kristi Anseth; Fiscal Year: 2009
    ..We will incorporate feedback control using a heuristic control loop to modify the biochemical and mechanical environment in order to optimize ECM production. ..
  2. Osteogenic Hydrogel Niches to Promote hMSC Migration and Differentiation
    Kristi S Anseth; Fiscal Year: 2011
    ..Our approach is to create 3-dimensional matrices that will act as scaffolds to recruit cells from surrounding tissues and promote natural bone regeneration processes, creating an improved and bioactive bone graft material. ..
  3. Bioactive Gels that Promote Long-Term Islet Survival and Function
    Kristi S Anseth; Fiscal Year: 2010
    ..If successful, this strategy will prolong the duration and function of transplanted pancreatic tissue without the need for life-long administration of immunosuppressive drugs. ..
  4. 3D Scaffolds for Controlled hMSC Differentiation
    Kristi Anseth; Fiscal Year: 2009
    ..These in vitro results will then provide the foundation to select formulations for testing in an animal model of bone regeneration in a critical-sized rat calvaria defect (aim 4). ..
  5. Bioactive Hydrogel Niches for 3D VIC Culture
    Kristi Anseth; Fiscal Year: 2009
    ..If successful, this strategy will prolong the duration and function of tissue-based valve replacements, especially for children. ..
  6. 3D Scaffolds for Controlled hMSC Differentiation
    Kristi Anseth; Fiscal Year: 2007
    ..These in vitro results will then provide the foundation to select formulations for testing in an animal model of bone regeneration in a critical-sized rat calvaria defect (aim 4). ..
  7. Photopolymerized Gels for Cartilage Tissue Engineering
    Kristi Anseth; Fiscal Year: 2006
    ..abstract_text> ..
  8. 3D Scaffolds for Controlled hMSC Differentiation
    Kristi Anseth; Fiscal Year: 2006
    ..These in vitro results will then provide the foundation to select formulations for testing in an animal model of bone regeneration in a critical-sized rat calvaria defect (aim 4). ..
  9. Online Monitoring and Control of Functional Cartilage Regeneration in Hydrogels
    Kristi Anseth; Fiscal Year: 2005
    ..We will incorporate feedback control using a heuristic control loop to modify the biochemical and mechanical environment in order to optimize ECM production. ..
  10. NOVEL TISSUE ENGINEERING MATRICES WITH CONTROLLED MICROS
    Kristi Anseth; Fiscal Year: 2000
    ..g., chondrocyte proliferation and extracellular matrix formation) and their performance in the correction of a cartilage defect to restore function (e.g., mechanical and eventual integrated tissue formation). ..
  11. Photopolymerized Gels for Cartilage Tissue Engineering
    Kristi Anseth; Fiscal Year: 2004
    ..abstract_text> ..
  12. NOVEL TISSUE ENGINEERING MATRICES WITH CONTROLLED MICROS
    Kristi Anseth; Fiscal Year: 1999
    ..g., chondrocyte proliferation and extracellular matrix formation) and their performance in the correction of a cartilage defect to restore function (e.g., mechanical and eventual integrated tissue formation). ..
  13. Bioactive Gels that Promote Long-Term Islet Survival and Function
    Kristi Anseth; Fiscal Year: 2009
    ..If successful, this strategy will prolong the duration and function of transplanted pancreatic tissue without the need for life-long administration of immunosuppressive drugs. ..
  14. NOVEL DEGRADABLE POLYMERS FOR ORTHOPEDIC APPLICATIONS
    Kristi Anseth; Fiscal Year: 2002
    ....
  15. Photopolymerized Gels for Cartilage Tissue Engineering
    Kristi Anseth; Fiscal Year: 2005
    ..abstract_text> ..
  16. Bioactive Hydrogel Niches for 3D VIC Culture
    Kristi S Anseth; Fiscal Year: 2010
    ..If successful, this strategy will prolong the duration and function of tissue-based valve replacements, especially for children. ..
  17. Online Monitoring and Control of Functional Cartilage Regeneration in Hydrogels
    Kristi Anseth; Fiscal Year: 2007
    ..We will incorporate feedback control using a heuristic control loop to modify the biochemical and mechanical environment in order to optimize ECM production. ..
  18. Photopolymerized Gels for Cartilage Tissue Engineering
    Kristi Anseth; Fiscal Year: 2002
    ..abstract_text> ..
  19. Online Monitoring and Control of Functional Cartilage Regeneration in Hydrogels
    Kristi Anseth; Fiscal Year: 2006
    ..We will incorporate feedback control using a heuristic control loop to modify the biochemical and mechanical environment in order to optimize ECM production. ..
  20. Photopolymerized Gels for Cartilage Tissue Engineering
    Kristi Anseth; Fiscal Year: 2003
    ..abstract_text> ..
  21. 3D Scaffolds for Controlled hMSC Differentiation
    Kristi Anseth; Fiscal Year: 2005
    ..These in vitro results will then provide the foundation to select formulations for testing in an animal model of bone regeneration in a critical-sized rat calvaria defect (aim 4). ..