Raul Andino

Summary

Affiliation: University of California
Country: USA

Publications

  1. pmc Cricket paralysis virus antagonizes Argonaute 2 to modulate antiviral defense in Drosophila
    Arabinda Nayak
    Department of Microbiology and Immunology, University of California, San Francisco, California, USA
    Nat Struct Mol Biol 17:547-54. 2010
  2. pmc Rationalizing the development of live attenuated virus vaccines
    Adam S Lauring
    Department of Medicine, University of California, San Francisco, California, USA
    Nat Biotechnol 28:573-9. 2010
  3. pmc The RNA silencing endonuclease Argonaute 2 mediates specific antiviral immunity in Drosophila melanogaster
    Ronald P Van Rij
    Department of Microbiology and Immunology, University of California at San Francisco, San Francisco, CA 94143, USA
    Genes Dev 20:2985-95. 2006
  4. pmc Antiviral immunity in Drosophila requires systemic RNA interference spread
    Maria Carla Saleh
    Department of Microbiology and Immunology, University of California, San Francisco 94122 2280, USA
    Nature 458:346-50. 2009
  5. pmc A morpholino oligomer targeting highly conserved internal ribosome entry site sequence is able to inhibit multiple species of picornavirus
    Jeffrey K Stone
    Department of Microbiology and Immunology, University of California at San Francisco, San Francisco, California 94143, USA
    Antimicrob Agents Chemother 52:1970-81. 2008
  6. pmc Harnessing endogenous miRNAs to control virus tissue tropism as a strategy for developing attenuated virus vaccines
    Dwight Barnes
    Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94143 2280, USA
    Cell Host Microbe 4:239-48. 2008
  7. pmc Global RNA structure analysis of poliovirus identifies a conserved RNA structure involved in viral replication and infectivity
    Cecily P Burrill
    Tetrad Graduate Program, University of California, San Francisco, California, USA
    J Virol 87:11670-83. 2013
  8. pmc An RNA element at the 5'-end of the poliovirus genome functions as a general promoter for RNA synthesis
    Dorothee A Vogt
    Department of Microbiology and Immunology, University of California, San Francisco, California, United States of America
    PLoS Pathog 6:e1000936. 2010
  9. ncbi request reprint Solution structure of a consensus stem-loop D RNA domain that plays important roles in regulating translation and replication in enteroviruses and rhinoviruses
    Zhihua Du
    Department of Pharmaceutical Chemistry, University of California, San Francisco, California 94143 2280, USA
    Biochemistry 43:11959-72. 2004
  10. ncbi request reprint Specific recognition of the C-rich strand of human telomeric DNA and the RNA template of human telomerase by the first KH domain of human poly(C)-binding protein-2
    Zhihua Du
    Pharmaceutical Chemistry and Microbiology and Immunology, University of California, San Francisco, California 94143 2280, USA
    J Biol Chem 279:48126-34. 2004

Collaborators

Detail Information

Publications42

  1. pmc Cricket paralysis virus antagonizes Argonaute 2 to modulate antiviral defense in Drosophila
    Arabinda Nayak
    Department of Microbiology and Immunology, University of California, San Francisco, California, USA
    Nat Struct Mol Biol 17:547-54. 2010
    ..In contrast, Sindbis pathogenesis was only modestly increased by expression of DCV- 1A. We conclude that RNAi suppressors function as virulence factors in insects and can target the Drosophila RNAi pathway at different points...
  2. pmc Rationalizing the development of live attenuated virus vaccines
    Adam S Lauring
    Department of Medicine, University of California, San Francisco, California, USA
    Nat Biotechnol 28:573-9. 2010
    ..Whereas these rationally attenuated viruses may lead to a new generation of safer, more widely applicable LAV vaccines, each approach requires further testing before progression to human testing...
  3. pmc The RNA silencing endonuclease Argonaute 2 mediates specific antiviral immunity in Drosophila melanogaster
    Ronald P Van Rij
    Department of Microbiology and Immunology, University of California at San Francisco, San Francisco, CA 94143, USA
    Genes Dev 20:2985-95. 2006
    ..Based on these results we propose that RNAi is a major antiviral immune defense mechanism in Drosophila...
  4. pmc Antiviral immunity in Drosophila requires systemic RNA interference spread
    Maria Carla Saleh
    Department of Microbiology and Immunology, University of California, San Francisco 94122 2280, USA
    Nature 458:346-50. 2009
    ..Thus, similar to protein-based immunity in vertebrates, the antiviral RNAi response in flies also relies on the systemic spread of a virus-specific immunity signal...
  5. pmc A morpholino oligomer targeting highly conserved internal ribosome entry site sequence is able to inhibit multiple species of picornavirus
    Jeffrey K Stone
    Department of Microbiology and Immunology, University of California at San Francisco, San Francisco, California 94143, USA
    Antimicrob Agents Chemother 52:1970-81. 2008
    ..These results suggest that EnteroX may have broad therapeutic potential against entero- and rhinoviruses...
  6. pmc Harnessing endogenous miRNAs to control virus tissue tropism as a strategy for developing attenuated virus vaccines
    Dwight Barnes
    Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94143 2280, USA
    Cell Host Microbe 4:239-48. 2008
    ..Importantly, these viruses retained the ability to replicate in nonneuronal tissues. As a result, these engineered miRNA-regulated viruses elicited strong protective immunity in mice without producing disease...
  7. pmc Global RNA structure analysis of poliovirus identifies a conserved RNA structure involved in viral replication and infectivity
    Cecily P Burrill
    Tetrad Graduate Program, University of California, San Francisco, California, USA
    J Virol 87:11670-83. 2013
    ....
  8. pmc An RNA element at the 5'-end of the poliovirus genome functions as a general promoter for RNA synthesis
    Dorothee A Vogt
    Department of Microbiology and Immunology, University of California, San Francisco, California, United States of America
    PLoS Pathog 6:e1000936. 2010
    ....
  9. ncbi request reprint Solution structure of a consensus stem-loop D RNA domain that plays important roles in regulating translation and replication in enteroviruses and rhinoviruses
    Zhihua Du
    Department of Pharmaceutical Chemistry, University of California, San Francisco, California 94143 2280, USA
    Biochemistry 43:11959-72. 2004
    ..The peculiar spatial arrangement of the distinctive structural elements implies that they may work synergistically to achieve optimal binding affinity and specificity toward the viral 3C or 3CD proteins...
  10. ncbi request reprint Specific recognition of the C-rich strand of human telomeric DNA and the RNA template of human telomerase by the first KH domain of human poly(C)-binding protein-2
    Zhihua Du
    Pharmaceutical Chemistry and Microbiology and Immunology, University of California, San Francisco, California 94143 2280, USA
    J Biol Chem 279:48126-34. 2004
    ..Specific interactions of PCBP2 KH1 with telomeric DNA and telomerase RNA suggest that PCBPs may participate in mechanisms involved in the regulation of telomere/telomerase functions...
  11. doi request reprint Engineering attenuated virus vaccines by controlling replication fidelity
    Marco Vignuzzi
    Department of Microbiology and Immunology, University of California San Francisco, 600 16th Street, San Francisco, California 94143 2280, USA
    Nat Med 14:154-61. 2008
    ..We propose that restricting viral quasispecies diversity provides a general approach for the rational design of stable, attenuated vaccines for a wide variety of viruses...
  12. pmc Codon usage determines the mutational robustness, evolutionary capacity, and virulence of an RNA virus
    Adam S Lauring
    Department of Medicine, University of California, San Francisco, San Francisco, CA 94143, USA
    Cell Host Microbe 12:623-32. 2012
    ..This organizing principle for RNA virus populations confers tolerance to mutations and facilitates replication and spread within the dynamic host environment...
  13. pmc The poliovirus replication machinery can escape inhibition by an antiviral drug that targets a host cell protein
    Shane Crotty
    Department of Microbiology and Immunology, University of California San Francisco, San Francisco, California 94143 2280, USA
    J Virol 78:3378-86. 2004
    ..Importantly, our data underscore the dramatic plasticity of the host-virus interactions required for successful viral replication...
  14. pmc Quasispecies diversity determines pathogenesis through cooperative interactions in a viral population
    Marco Vignuzzi
    Department of Microbiology and Immunology, University of California, San Francisco, California 94143 2280, USA
    Nature 439:344-8. 2006
    ..Our study provides direct evidence for a fundamental prediction of the quasispecies theory and establishes a link between mutation rate, population dynamics and pathogenesis...
  15. pmc An enhanced single base extension technique for the analysis of complex viral populations
    Dale R Webster
    Biological and Medical Informatics Program, University of California San Francisco, San Francisco, California, United States of America
    PLoS ONE 4:e7453. 2009
    ....
  16. pmc Manganese-dependent polioviruses caused by mutations within the viral polymerase
    Shane Crotty
    Department of Microbiology and Immunology, University of California, San Francisco 94143 0414, USA
    J Virol 77:5378-88. 2003
    ....
  17. ncbi request reprint The silent treatment: RNAi as a defense against virus infection in mammals
    Ronald P Van Rij
    Department of Microbiology and Immunology, University of California San Francisco, San Francisco, CA 94143 2280, USA
    Trends Biotechnol 24:186-93. 2006
    ..Although several issues remain to be addressed, such as delivery and viral escape, these findings hold tremendous potential for the development of RNAi-based antiviral therapeutics...
  18. ncbi request reprint Short interfering RNA confers intracellular antiviral immunity in human cells
    Leonid Gitlin
    Department of Microbiology and Immunology, Box 0414 and Program in Neuroscience, University of California, San Francisco, California 94143 0414, USA
    Nature 418:430-4. 2002
    ..Thus, siRNAs elicit specific intracellular antiviral resistance that may provide a therapeutic strategy against human viruses...
  19. pmc Single-cell analysis uncovers extensive biological noise in poliovirus replication
    Michael B Schulte
    Tetrad Graduate Program, University of California, San Francisco, California, USA
    J Virol 88:6205-12. 2014
    ..The distinct nature of stochasticity in the outcome of infection by low and high numbers of viral particles may have important implications for our understanding of the determinants of successful viral infections...
  20. pmc The endocytic pathway mediates cell entry of dsRNA to induce RNAi silencing
    Maria Carla Saleh
    Department of Microbiology and Immunology, University of California San Francisco, CA 94143 2280, USA
    Nat Cell Biol 8:793-802. 2006
    ..The possible role of these receptors in dsRNA entry may link RNA interference (RNAi) silencing to other innate immune responses...
  21. pmc Mutational and fitness landscapes of an RNA virus revealed through population sequencing
    Ashley Acevedo
    Department of Microbiology and Immunology, University of California, San Francisco, California 94122 2280, USA
    Nature 505:686-90. 2014
    ..To our knowledge, our study provides the first single-nucleotide fitness landscape of an evolving RNA virus and establishes a general experimental platform for studying the genetic changes underlying the evolution of virus populations. ..
  22. pmc Nucleic acid-based immune system: the antiviral potential of mammalian RNA silencing
    Leonid Gitlin
    Department of Microbiology and Immunology, University of California, San Francisco, California 94143 2280, USA
    J Virol 77:7159-65. 2003
  23. ncbi request reprint Implications of high RNA virus mutation rates: lethal mutagenesis and the antiviral drug ribavirin
    Shane Crotty
    Department of Microbiology and Immunology, University of California, San Francisco 94143, USA
    Microbes Infect 4:1301-7. 2002
    ..In this review we discuss the genetic explanations, evolutionary implications, and drug development opportunities associated with RNA virus mutagenesis...
  24. doi request reprint RNA interference-mediated intrinsic antiviral immunity in invertebrates
    Arabinda Nayak
    Department of Microbiology and Immunology, University of California, San Francisco, CA 94122 2280, USA
    Curr Top Microbiol Immunol 371:183-200. 2013
    ..This has led to a deeper understanding of fundamental aspects of small RNA-based antiviral immunity in invertebrates and its contribution to viral spread and pathogenesis...
  25. doi request reprint Slicer activity in Drosophila melanogaster S2 extract
    Arabinda Nayak
    Department of Microbiology and Immunology, University of California, San Francisco, CA, USA
    Methods Mol Biol 721:231-44. 2011
    ..In this chapter, we will describe how to prepare enzymatically active cell-free S2 extract to analyze the Slicer activity of RISC as well as the effects of viral RNAi suppressors which block this process...
  26. pmc Exploring the fitness landscape of an RNA virus by using a universal barcode microarray
    Adam S Lauring
    Department of Medicine, UCSF Box 2280, San Francisco, CA 94143 2280, USA
    J Virol 85:3780-91. 2011
    ..The experimental paradigm presented here is readily adaptable to other viral systems and can potentially be used to track thousands of variants in a cost-effective manner...
  27. pmc Poliovirus escape from RNA interference: short interfering RNA-target recognition and implications for therapeutic approaches
    Leonid Gitlin
    Department of Microbiology and Immunology, University of California San Francisco, Mission Bay Genentech Hall, Box 2280, San Francisco, CA 94143 2280, USA
    J Virol 79:1027-35. 2005
    ..Our study uncovers the elegant precision of target recognition by the RNAi machinery and provides the basis for the development of effective RNAi-based therapies that prevent viral escape...
  28. ncbi request reprint Ribavirin and lethal mutagenesis of poliovirus: molecular mechanisms, resistance and biological implications
    Marco Vignuzzi
    Department of Microbiology and Immunology, University of California, Mission Bay, Genentech Hall, Box 2280, San Francisco CA 94143 2280, USA
    Virus Res 107:173-81. 2005
    ....
  29. doi request reprint Poliovirus: generation, quantification, propagation, purification, and storage
    Cecily P Burrill
    Department of Microbiology and Immunology, University of California, San Francisco, California, USA
    Curr Protoc Microbiol . 2013
    ..PV infects many human and non-human primate cell lines including HeLa and HeLa S3 cells, and can grow to high titer in culture. Protocols for the production, propagation, quantification, and purification of PV are presented...
  30. pmc Quasispecies theory and the behavior of RNA viruses
    Adam S Lauring
    Department of Medicine, University of California, San Francisco, San Francisco, California, United States of America
    PLoS Pathog 6:e1001005. 2010
    ..Here, we discuss basic principles of quasispecies theory and describe its relevance for our understanding of viral fitness, virulence, and antiviral therapeutic strategy...
  31. pmc Non-specific dsRNA-mediated antiviral response in the honey bee
    Michelle L Flenniken
    Department of Microbiology and Immunology, University of California San Francisco, San Francisco, California, United States of America Department of Plant Sciences and Plant Pathology, Montana State University, Bozeman, Montana, United States of America
    PLoS ONE 8:e77263. 2013
    ..Our results suggest that dsRNA, a viral pathogen associated molecular pattern (PAMP), triggers an antiviral response that controls virus infection in honey bees. ..
  32. ncbi request reprint Poliovirus infection blocks ERGIC-to-Golgi trafficking and induces microtubule-dependent disruption of the Golgi complex
    Oren Beske
    Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94143, USA
    J Cell Sci 120:3207-18. 2007
    ..Instead, these phenomena are independent effects of poliovirus infection on the host secretory complex...
  33. ncbi request reprint Poliovirus pathogenesis in a new poliovirus receptor transgenic mouse model: age-dependent paralysis and a mucosal route of infection
    Shane Crotty
    Department of Microbiology and Immunology, University of California, Box 0414, San Francisco, CA 94143, USA
    J Gen Virol 83:1707-20. 2002
    ..Intranasally infected mice frequently display unusual neurological behaviours. The PVR transgenic mouse reported here provides the first available model for a mucosal route of infection with poliovirus...
  34. ncbi request reprint Extending the family of UNCG-like tetraloop motifs: NMR structure of a CACG tetraloop from coxsackievirus B3
    Zhihua Du
    Department of Pharmaceutical Chemistry, University of California, San Francisco, California 94143 0446, USA
    Biochemistry 42:4373-83. 2003
    ..The U.G base pair closing the stem and the A residue in the loop introduce some small structural and themodynamic distinctions from the canonical cUUCGg tetraloop that may be important for recognition by the viral 3C protease...
  35. pmc Hsp90 inhibitors exhibit resistance-free antiviral activity against respiratory syncytial virus
    Ron Geller
    Department of Biology, Stanford University, Stanford, California, United States of America
    PLoS ONE 8:e56762. 2013
    ....
  36. ncbi request reprint Poliovirus vaccine strains as mucosal vaccine vectors and their potential use to develop an AIDS vaccine
    Shane Crotty
    Department of Microbiology and Immunology, University of California, 513 Parnassus Ave, Box 0414, San Francisco, CA 94143, USA
    Adv Drug Deliv Rev 56:835-52. 2004
    ..Most importantly, we have demonstrated that vaccination of cynomolgus monkeys with poliovirus-SIV cocktails protects against infection and AIDS after intravaginal challenge with highly pathogenic SIV(mac)251...
  37. ncbi request reprint Library preparation for highly accurate population sequencing of RNA viruses
    Ashley Acevedo
    Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, California, USA
    Nat Protoc 9:1760-9. 2014
    ..Although library preparation takes ∼5 d, the high-quality data generated by CirSeq simplifies downstream data analysis, making this approach substantially more tractable for experimentalists. ..
  38. ncbi request reprint RNA silencing in viral infections: insights from poliovirus
    Maria Carla Saleh
    Department of Microbiology and Immunology, University of California, 513 Parnassus Avenue, Box 0414, San Francisco, CA 94143 2280, USA
    Virus Res 102:11-7. 2004
    ..Here we review and discuss the current data and perspectives on the use of RNA interference in the study of poliovirus as a model for positive strand RNA viruses...
  39. ncbi request reprint NMR structures of loop B RNAs from the stem-loop IV domain of the enterovirus internal ribosome entry site: a single C to U substitution drastically changes the shape and flexibility of RNA
    Zhihua Du
    Department of Pharmaceutical Chemistry, University of California, San Francisco, California 94143 2280, USA
    Biochemistry 43:5757-71. 2004
    ....
  40. ncbi request reprint RNA Structure Analysis of Viruses Using SHAPE
    Cecily P Burrill
    Department of Microbiology and Immunology, University of California, San Francisco, California
    Curr Protoc Microbiol 30:15H.3.1-15H.3.12. 2013
    ..These include methods for nondenaturing RNA extraction, RNA modification and primer extension, and data processing in ShapeFinder. Curr. Protoc. Microbiol. 30:15H.3.1-15H.3.12. © 2013 by John Wiley & Sons, Inc. ..
  41. doi request reprint Poliovirus: generation and characterization of mutants
    Cecily P Burrill
    Department of Microbiology and Immunology, University of California, San Francisco, California, USA
    Curr Protoc Microbiol . 2013
    ..The ease of genetic studies in poliovirus is a primary reason that it has long served as a model to study RNA virus biology, pathogenesis, and evolution. Protocols for the generation and characterization of PV mutants are presented...
  42. pmc Identification and manipulation of the molecular determinants influencing poliovirus recombination
    Charles Runckel
    Howard Hughes Medical Institute, Bethesda, Maryland, United States of America
    PLoS Pathog 9:e1003164. 2013
    ..Our results imply that modification of these motifs could be extended to whole genome re-designs for the development of recombination-deficient, genetically stable live vaccine strains...

Research Grants31

  1. RNAi as an Intercellular Antiviral Defense Mechanism
    Raul Andino; Fiscal Year: 2010
    ..abstract_text> ..
  2. Genetic Diversity as a Determinant of Viral Pathogenesis and Attenuation
    Raul Andino; Fiscal Year: 2010
    ....
  3. Genetic Diversity as a Determinant of Viral Pathogenesis and Attenuation
    Raul Andino; Fiscal Year: 2009
    ....
  4. RNA PROTEIN INTERACTIONS IN POLIOVIRUS REPLICATION
    Raul Andino; Fiscal Year: 2005
    ..These studies may yield new ways to rationally design anti-poliovirus drugs. ..
  5. RECOMBINANT PICORNAVIRUSES AS AIDS VACCINES
    Raul Andino; Fiscal Year: 2004
    ..abstract_text> ..
  6. RECOMBINANT PICORNAVIRUSES AS AIDS VACCINES
    Raul Andino; Fiscal Year: 2003
    ..abstract_text> ..
  7. RNA PROTEIN INTERACTIONS IN POLIOVIRUS REPLICATION
    Raul Andino; Fiscal Year: 2003
    ..These studies may yield new ways to rationally design anti-poliovirus drugs. ..
  8. RNA PROTEIN INTERACTIONS IN POLIOVIRUS REPLICATION
    Raul Andino; Fiscal Year: 2000
    ..The specific aims of this proposal are to determine the structure of the NRP-B complex, to study its function in oocytes and human cells, and to characterize human factors require for poliovirus replication in oocytes. ..
  9. RECOMBINANT PICORNAVIRUSES AS AIDS VACCINES
    Raul Andino; Fiscal Year: 1999
    ....
  10. RNA PROTEIN INTERACTIONS IN POLIOVIRUS REPLICATION
    Raul Andino; Fiscal Year: 2007
    ..These studies may yield new ways to rationally design anti-poliovirus drugs. ..
  11. Genetic Diversity as a Determinant of Viral Pathogenesis and Attenuation
    Raul Andino; Fiscal Year: 2007
    ....
  12. RNAi as an Intercellular Antiviral Defense Mechanism
    Raul Andino; Fiscal Year: 2009
    ..abstract_text> ..
  13. Genetic Diversity as a Determinant of Viral Pathogenesis and Attenuation
    Raul Andino; Fiscal Year: 2009
    ....
  14. RNAi as an Intercellular Antiviral Defense Mechanism
    Raul Andino; Fiscal Year: 2007
    ....
  15. RNAi as an Intercellular Antiviral Defense Mechanism
    Raul Andino; Fiscal Year: 2006
    ....
  16. RECOMBINANT PICORNAVIRUSES AS AIDS VACCINES
    Raul Andino; Fiscal Year: 2006
    ..abstract_text> ..
  17. RNA PROTEIN INTERACTIONS IN POLIOVIRUS REPLICATION
    Raul Andino; Fiscal Year: 2001
    ..The specific aims of this proposal are to determine the structure of the NRP-B complex, to study its function in oocytes and human cells, and to characterize human factors require for poliovirus replication in oocytes. ..
  18. RECOMBINANT PICORNAVIRUSES AS AIDS VACCINES
    Raul Andino; Fiscal Year: 2002
    ..abstract_text> ..
  19. RNA PROTEIN INTERACTIONS IN POLIOVIRUS REPLICATION
    Raul Andino; Fiscal Year: 2002
    ..The specific aims of this proposal are to determine the structure of the NRP-B complex, to study its function in oocytes and human cells, and to characterize human factors require for poliovirus replication in oocytes. ..
  20. RNA PROTEIN INTERACTIONS IN POLIOVIRUS REPLICATION
    Raul Andino; Fiscal Year: 1999
    ..The specific aims of this proposal are to determine the structure of the NRP-B complex, to study its function in oocytes and human cells, and to characterize human factors require for poliovirus replication in oocytes. ..
  21. RNA PROTEIN INTERACTIONS IN POLIOVIRUS REPLICATION
    Raul Andino; Fiscal Year: 2004
    ..These studies may yield new ways to rationally design anti-poliovirus drugs. ..
  22. RECOMBINANT PICORNAVIRUSES AS AIDS VACCINES
    Raul Andino; Fiscal Year: 2005
    ..abstract_text> ..
  23. RNA PROTEIN INTERACTIONS IN POLIOVIRUS REPLICATION
    Raul Andino; Fiscal Year: 2006
    ..These studies may yield new ways to rationally design anti-poliovirus drugs. ..
  24. Mechanism of poliovirus replication
    Raul Andino; Fiscal Year: 2010
    ..Understanding RNA recombination will help us to understand virus diversity, evolution and pathogenesis, and it may allow the development of safe and effective vaccines and antiviral drugs. ..