Raul Andino

Summary

Affiliation: University of California
Country: USA

Publications

  1. pmc Rationalizing the development of live attenuated virus vaccines
    Adam S Lauring
    Department of Medicine, University of California, San Francisco, California, USA
    Nat Biotechnol 28:573-9. 2010
  2. pmc Cricket paralysis virus antagonizes Argonaute 2 to modulate antiviral defense in Drosophila
    Arabinda Nayak
    Department of Microbiology and Immunology, University of California, San Francisco, California, USA
    Nat Struct Mol Biol 17:547-54. 2010
  3. doi request reprint Antiviral immunity in Drosophila requires systemic RNA interference spread
    Maria Carla Saleh
    Department of Microbiology and Immunology, University of California, San Francisco 94122 2280, USA
    Nature 458:346-50. 2009
  4. pmc Global RNA structure analysis of poliovirus identifies a conserved RNA structure involved in viral replication and infectivity
    Cecily P Burrill
    Tetrad Graduate Program, University of California, San Francisco, California, USA
    J Virol 87:11670-83. 2013
  5. pmc Codon usage determines the mutational robustness, evolutionary capacity, and virulence of an RNA virus
    Adam S Lauring
    Department of Medicine, University of California, San Francisco, San Francisco, CA 94143, USA
    Cell Host Microbe 12:623-32. 2012
  6. pmc Harnessing endogenous miRNAs to control virus tissue tropism as a strategy for developing attenuated virus vaccines
    Dwight Barnes
    Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94143 2280, USA
    Cell Host Microbe 4:239-48. 2008
  7. doi request reprint RNA interference-mediated intrinsic antiviral immunity in invertebrates
    Arabinda Nayak
    Department of Microbiology and Immunology, University of California, San Francisco, CA 94122 2280, USA
    Curr Top Microbiol Immunol 371:183-200. 2013
  8. pmc Exploring the fitness landscape of an RNA virus by using a universal barcode microarray
    Adam S Lauring
    Department of Medicine, UCSF Box 2280, San Francisco, CA 94143 2280, USA
    J Virol 85:3780-91. 2011
  9. doi request reprint Slicer activity in Drosophila melanogaster S2 extract
    Arabinda Nayak
    Department of Microbiology and Immunology, University of California, San Francisco, CA, USA
    Methods Mol Biol 721:231-44. 2011
  10. pmc An RNA element at the 5'-end of the poliovirus genome functions as a general promoter for RNA synthesis
    Dorothee A Vogt
    Department of Microbiology and Immunology, University of California, San Francisco, California, United States of America
    PLoS Pathog 6:e1000936. 2010

Collaborators

  • Adam S Lauring
  • Kalle Saksela
  • Hao Li
  • Jeremy O Jones
  • David A Stein
  • Cecily P Burrill
  • Arabinda Nayak
  • Ronald P Van Rij
  • Maria Carla Saleh
  • Vanessa R Strings
  • Michel Tassetto
  • Mark Kunitomi
  • Christophe Antoniewski
  • Bassam Berry
  • Marco Vignuzzi
  • Michael B Schulte
  • Oscar Westesson
  • Charles Runckel
  • Joseph L Derisi
  • Dorothee A Vogt
  • Dale R Webster
  • Dwight Barnes
  • Jeffrey K Stone
  • Mark Segal
  • Ashley Acevedo
  • Changhui Deng
  • Andrew Krutchinsky
  • John Gross
  • ValĂ©rie Gausson
  • Kael F Fischer
  • Bertsy Goic
  • Armin G Hekele
  • Caroline Jacquier
  • Rene Rijnbrand
  • Patrick L Iversen
  • Yinghong Ma
  • Yan Yang
  • Emily Wendt
  • Andrew Houk
  • Catherine Foo

Detail Information

Publications20

  1. pmc Rationalizing the development of live attenuated virus vaccines
    Adam S Lauring
    Department of Medicine, University of California, San Francisco, California, USA
    Nat Biotechnol 28:573-9. 2010
    ..Whereas these rationally attenuated viruses may lead to a new generation of safer, more widely applicable LAV vaccines, each approach requires further testing before progression to human testing...
  2. pmc Cricket paralysis virus antagonizes Argonaute 2 to modulate antiviral defense in Drosophila
    Arabinda Nayak
    Department of Microbiology and Immunology, University of California, San Francisco, California, USA
    Nat Struct Mol Biol 17:547-54. 2010
    ..In contrast, Sindbis pathogenesis was only modestly increased by expression of DCV- 1A. We conclude that RNAi suppressors function as virulence factors in insects and can target the Drosophila RNAi pathway at different points...
  3. doi request reprint Antiviral immunity in Drosophila requires systemic RNA interference spread
    Maria Carla Saleh
    Department of Microbiology and Immunology, University of California, San Francisco 94122 2280, USA
    Nature 458:346-50. 2009
    ..Thus, similar to protein-based immunity in vertebrates, the antiviral RNAi response in flies also relies on the systemic spread of a virus-specific immunity signal...
  4. pmc Global RNA structure analysis of poliovirus identifies a conserved RNA structure involved in viral replication and infectivity
    Cecily P Burrill
    Tetrad Graduate Program, University of California, San Francisco, California, USA
    J Virol 87:11670-83. 2013
    ....
  5. pmc Codon usage determines the mutational robustness, evolutionary capacity, and virulence of an RNA virus
    Adam S Lauring
    Department of Medicine, University of California, San Francisco, San Francisco, CA 94143, USA
    Cell Host Microbe 12:623-32. 2012
    ..This organizing principle for RNA virus populations confers tolerance to mutations and facilitates replication and spread within the dynamic host environment...
  6. pmc Harnessing endogenous miRNAs to control virus tissue tropism as a strategy for developing attenuated virus vaccines
    Dwight Barnes
    Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94143 2280, USA
    Cell Host Microbe 4:239-48. 2008
    ..Importantly, these viruses retained the ability to replicate in nonneuronal tissues. As a result, these engineered miRNA-regulated viruses elicited strong protective immunity in mice without producing disease...
  7. doi request reprint RNA interference-mediated intrinsic antiviral immunity in invertebrates
    Arabinda Nayak
    Department of Microbiology and Immunology, University of California, San Francisco, CA 94122 2280, USA
    Curr Top Microbiol Immunol 371:183-200. 2013
    ..This has led to a deeper understanding of fundamental aspects of small RNA-based antiviral immunity in invertebrates and its contribution to viral spread and pathogenesis...
  8. pmc Exploring the fitness landscape of an RNA virus by using a universal barcode microarray
    Adam S Lauring
    Department of Medicine, UCSF Box 2280, San Francisco, CA 94143 2280, USA
    J Virol 85:3780-91. 2011
    ..The experimental paradigm presented here is readily adaptable to other viral systems and can potentially be used to track thousands of variants in a cost-effective manner...
  9. doi request reprint Slicer activity in Drosophila melanogaster S2 extract
    Arabinda Nayak
    Department of Microbiology and Immunology, University of California, San Francisco, CA, USA
    Methods Mol Biol 721:231-44. 2011
    ..In this chapter, we will describe how to prepare enzymatically active cell-free S2 extract to analyze the Slicer activity of RISC as well as the effects of viral RNAi suppressors which block this process...
  10. pmc An RNA element at the 5'-end of the poliovirus genome functions as a general promoter for RNA synthesis
    Dorothee A Vogt
    Department of Microbiology and Immunology, University of California, San Francisco, California, United States of America
    PLoS Pathog 6:e1000936. 2010
    ....
  11. pmc An enhanced single base extension technique for the analysis of complex viral populations
    Dale R Webster
    Biological and Medical Informatics Program, University of California San Francisco, San Francisco, California, United States of America
    PLoS ONE 4:e7453. 2009
    ....
  12. doi request reprint Poliovirus: generation, quantification, propagation, purification, and storage
    Cecily P Burrill
    Department of Microbiology and Immunology, University of California, San Francisco, California, USA
    Curr Protoc Microbiol . 2013
    ..PV infects many human and non-human primate cell lines including HeLa and HeLa S3 cells, and can grow to high titer in culture. Protocols for the production, propagation, quantification, and purification of PV are presented...
  13. pmc A morpholino oligomer targeting highly conserved internal ribosome entry site sequence is able to inhibit multiple species of picornavirus
    Jeffrey K Stone
    Department of Microbiology and Immunology, University of California at San Francisco, San Francisco, California 94143, USA
    Antimicrob Agents Chemother 52:1970-81. 2008
    ..These results suggest that EnteroX may have broad therapeutic potential against entero- and rhinoviruses...
  14. doi request reprint Engineering attenuated virus vaccines by controlling replication fidelity
    Marco Vignuzzi
    Department of Microbiology and Immunology, University of California San Francisco, 600 16th Street, San Francisco, California 94143 2280, USA
    Nat Med 14:154-61. 2008
    ..We propose that restricting viral quasispecies diversity provides a general approach for the rational design of stable, attenuated vaccines for a wide variety of viruses...
  15. pmc Quasispecies theory and the behavior of RNA viruses
    Adam S Lauring
    Department of Medicine, University of California, San Francisco, San Francisco, California, United States of America
    PLoS Pathog 6:e1001005. 2010
    ..Here, we discuss basic principles of quasispecies theory and describe its relevance for our understanding of viral fitness, virulence, and antiviral therapeutic strategy...
  16. pmc The RNA silencing endonuclease Argonaute 2 mediates specific antiviral immunity in Drosophila melanogaster
    Ronald P Van Rij
    Department of Microbiology and Immunology, University of California at San Francisco, San Francisco, CA 94143, USA
    Genes Dev 20:2985-95. 2006
    ..Based on these results we propose that RNAi is a major antiviral immune defense mechanism in Drosophila...
  17. doi request reprint Poliovirus: generation and characterization of mutants
    Cecily P Burrill
    Department of Microbiology and Immunology, University of California, San Francisco, California, USA
    Curr Protoc Microbiol . 2013
    ..The ease of genetic studies in poliovirus is a primary reason that it has long served as a model to study RNA virus biology, pathogenesis, and evolution. Protocols for the generation and characterization of PV mutants are presented...
  18. pmc Identification and manipulation of the molecular determinants influencing poliovirus recombination
    Charles Runckel
    Howard Hughes Medical Institute, Bethesda, Maryland, United States of America
    PLoS Pathog 9:e1003164. 2013
    ..Our results imply that modification of these motifs could be extended to whole genome re-designs for the development of recombination-deficient, genetically stable live vaccine strains...
  19. ncbi request reprint The silent treatment: RNAi as a defense against virus infection in mammals
    Ronald P Van Rij
    Department of Microbiology and Immunology, University of California San Francisco, San Francisco, CA 94143 2280, USA
    Trends Biotechnol 24:186-93. 2006
    ..Although several issues remain to be addressed, such as delivery and viral escape, these findings hold tremendous potential for the development of RNAi-based antiviral therapeutics...
  20. ncbi request reprint RNA silencing in viral infections: insights from poliovirus
    Maria Carla Saleh
    Department of Microbiology and Immunology, University of California, 513 Parnassus Avenue, Box 0414, San Francisco, CA 94143 2280, USA
    Virus Res 102:11-7. 2004
    ..Here we review and discuss the current data and perspectives on the use of RNA interference in the study of poliovirus as a model for positive strand RNA viruses...

Research Grants31

  1. RNAi as an Intercellular Antiviral Defense Mechanism
    Raul Andino; Fiscal Year: 2010
    ..abstract_text> ..
  2. Genetic Diversity as a Determinant of Viral Pathogenesis and Attenuation
    Raul Andino; Fiscal Year: 2010
    ....
  3. RECOMBINANT PICORNAVIRUSES AS AIDS VACCINES
    Raul Andino; Fiscal Year: 2006
    ..abstract_text> ..
  4. RNAi as an Intercellular Antiviral Defense Mechanism
    Raul Andino; Fiscal Year: 2007
    ....
  5. RNA PROTEIN INTERACTIONS IN POLIOVIRUS REPLICATION
    Raul Andino; Fiscal Year: 2007
    ..These studies may yield new ways to rationally design anti-poliovirus drugs. ..
  6. Genetic Diversity as a Determinant of Viral Pathogenesis and Attenuation
    Raul Andino; Fiscal Year: 2007
    ....
  7. RNAi as an Intercellular Antiviral Defense Mechanism
    Raul Andino; Fiscal Year: 2009
    ..abstract_text> ..
  8. Genetic Diversity as a Determinant of Viral Pathogenesis and Attenuation
    Raul Andino; Fiscal Year: 2009
    ....
  9. Genetic Diversity as a Determinant of Viral Pathogenesis and Attenuation
    Raul Andino; Fiscal Year: 2009
    ....
  10. RECOMBINANT PICORNAVIRUSES AS AIDS VACCINES
    Raul Andino; Fiscal Year: 1999
    ....
  11. RNA PROTEIN INTERACTIONS IN POLIOVIRUS REPLICATION
    Raul Andino; Fiscal Year: 2002
    ..The specific aims of this proposal are to determine the structure of the NRP-B complex, to study its function in oocytes and human cells, and to characterize human factors require for poliovirus replication in oocytes. ..
  12. Mechanism of poliovirus replication
    Raul Andino; Fiscal Year: 2010
    ..Understanding RNA recombination will help us to understand virus diversity, evolution and pathogenesis, and it may allow the development of safe and effective vaccines and antiviral drugs. ..