JAMES ALWINE

Summary

Affiliation: University of Pennsylvania
Country: USA

Publications

  1. pmc Human cytomegalovirus infection induces adipocyte-like lipogenesis through activation of sterol regulatory element binding protein 1
    Yongjun Yu
    Department of Cancer Biology, Abramson Family Cancer Research Institute, University of Pennsylvania, School of Medicine, Philadelphia, Pennsylvania, USA
    J Virol 86:2942-9. 2012
  2. ncbi request reprint Modulation of host cell stress responses by human cytomegalovirus
    J C Alwine
    Department of Cancer Biology, University of Pennsylvania, 314 Biomedical Research Building, 421 Curie Blvd Philadelphia, PA 19104 6142, USA
    Curr Top Microbiol Immunol 325:263-79. 2008
  3. pmc Human cytomegalovirus induces multiple means to combat reactive oxygen species
    CARISA TILTON
    Department of Cancer Biology, Abramson Family Cancer Research Institute, School of Medicine, University of Pennsylvania, Philadelphia, PA 19104 6142, USA
    J Virol 85:12585-93. 2011
  4. pmc Human cytomegalovirus infection maintains mTOR activity and its perinuclear localization during amino acid deprivation
    AMY J CLIPPINGER
    Department of Cancer Biology, 314 Biomedical Research Building, 421 Curie Blvd, School of Medicine, University of Pennsylvania, Philadelphia, PA 19104 6142, USA
    J Virol 85:9369-76. 2011
  5. pmc Human cytomegalovirus activates glucose transporter 4 expression to increase glucose uptake during infection
    Yongjun Yu
    Department of Cancer Biology, Abramson Family Cancer Research Institute, University of Pennsylvania, School of Medicine, Philadelphia, Pennsylvania 19104 6142, USA
    J Virol 85:1573-80. 2011
  6. pmc The changing role of mTOR kinase in the maintenance of protein synthesis during human cytomegalovirus infection
    AMY J CLIPPINGER
    Department of Cancer Biology, 314 Biomedical Research Building, 421 Curie Blvd, School of Medicine, University of Pennsylvania, Philadelphia, PA 19104 6142, USA
    J Virol 85:3930-9. 2011
  7. pmc Glutamine metabolism is essential for human cytomegalovirus infection
    Jeremy W Chambers
    Department of Cancer Biology, 314 Biomedical Research Building, 421 Curie Blvd, School of Medicine, University of Pennsylvania, Philadelphia, PA 19104 6142, USA
    J Virol 84:1867-73. 2010
  8. pmc Human cytomegalovirus infection induces rapamycin-insensitive phosphorylation of downstream effectors of mTOR kinase
    Sagar B Kudchodkar
    Department of Cancer Biology, Abramson Family Cancer Research Institute, 421 Curie Blvd, University of Pennsylvania, Philadelphia, PA 19104 6142, USA
    J Virol 78:11030-9. 2004
  9. pmc The TORrid affairs of viruses: effects of mammalian DNA viruses on the PI3K-Akt-mTOR signalling pathway
    Nicholas J Buchkovich
    Department of Cancer Biology and Abramson Family Cancer Research Institute, University of Pennsylvania, 314 Biomedical Research Building, 421 Curie Blvd, Philadelphia, 19104 6142 Pennsylvania, USA
    Nat Rev Microbiol 6:266-75. 2008
  10. pmc Human cytomegalovirus specifically controls the levels of the endoplasmic reticulum chaperone BiP/GRP78, which is required for virion assembly
    Nicholas J Buchkovich
    Department of Cancer Biology, 314 Biomedical Research Building, 421 Curie Blvd, School of Medicine, University of Pennsylvania, Philadelphia, PA 19104 6142, USA
    J Virol 82:31-9. 2008

Research Grants

Collaborators

  • Yongjun Yu
  • Tobi G Maguire
  • Sagar B Kudchodkar
  • AMY J CLIPPINGER
  • Jennifer A Isler
  • Nicholas J Buchkovich
  • M Inmaculada Barrasa
  • CARISA TILTON
  • Jeremy W Chambers
  • Sita Awasthi
  • Julie A Heider
  • Tobi Maguire
  • Adrienne W Paton
  • Carisa A Zampieri
  • James C Paton
  • Gregory Q Del Prete
  • Noam Y Harel
  • Tobi Goldberg Maguire
  • Alison H Skalet
  • Noam Harel
  • Thomas Shenk

Detail Information

Publications21

  1. pmc Human cytomegalovirus infection induces adipocyte-like lipogenesis through activation of sterol regulatory element binding protein 1
    Yongjun Yu
    Department of Cancer Biology, Abramson Family Cancer Research Institute, University of Pennsylvania, School of Medicine, Philadelphia, Pennsylvania, USA
    J Virol 86:2942-9. 2012
    ..Our studies suggest that HCMV induces adipocyte-like lipogenesis and overrides normal sterol feedback controls in order to maintain high levels of constitutive lipid synthesis during infection...
  2. ncbi request reprint Modulation of host cell stress responses by human cytomegalovirus
    J C Alwine
    Department of Cancer Biology, University of Pennsylvania, 314 Biomedical Research Building, 421 Curie Blvd Philadelphia, PA 19104 6142, USA
    Curr Top Microbiol Immunol 325:263-79. 2008
    ..Such dramatic alterations of this pathway lead to questions of what other beneficial effects the virus might gain from these changes and how these changes may contribute to HCMV pathogenesis...
  3. pmc Human cytomegalovirus induces multiple means to combat reactive oxygen species
    CARISA TILTON
    Department of Cancer Biology, Abramson Family Cancer Research Institute, School of Medicine, University of Pennsylvania, Philadelphia, PA 19104 6142, USA
    J Virol 85:12585-93. 2011
    ..Overall, our data suggest that HCMV utilizes virus-specific mechanisms to activate a variety of means to protect the cell and mTORC1 from the effects of ROS...
  4. pmc Human cytomegalovirus infection maintains mTOR activity and its perinuclear localization during amino acid deprivation
    AMY J CLIPPINGER
    Department of Cancer Biology, 314 Biomedical Research Building, 421 Curie Blvd, School of Medicine, University of Pennsylvania, Philadelphia, PA 19104 6142, USA
    J Virol 85:9369-76. 2011
    ....
  5. pmc Human cytomegalovirus activates glucose transporter 4 expression to increase glucose uptake during infection
    Yongjun Yu
    Department of Cancer Biology, Abramson Family Cancer Research Institute, University of Pennsylvania, School of Medicine, Philadelphia, Pennsylvania 19104 6142, USA
    J Virol 85:1573-80. 2011
    ..Our data show that HCMV-specific mechanisms are used to replace GLUT1, the normal HF GLUT, with GLUT4, the major glucose transporter in adipose tissue, which has a 3-fold-higher glucose transport capacity...
  6. pmc The changing role of mTOR kinase in the maintenance of protein synthesis during human cytomegalovirus infection
    AMY J CLIPPINGER
    Department of Cancer Biology, 314 Biomedical Research Building, 421 Curie Blvd, School of Medicine, University of Pennsylvania, Philadelphia, PA 19104 6142, USA
    J Virol 85:3930-9. 2011
    ..Overall, our data suggest a dynamic relationship between HCMV and mTOR kinase which changes during the course of infection...
  7. pmc Glutamine metabolism is essential for human cytomegalovirus infection
    Jeremy W Chambers
    Department of Cancer Biology, 314 Biomedical Research Building, 421 Curie Blvd, School of Medicine, University of Pennsylvania, Philadelphia, PA 19104 6142, USA
    J Virol 84:1867-73. 2010
    ....
  8. pmc Human cytomegalovirus infection induces rapamycin-insensitive phosphorylation of downstream effectors of mTOR kinase
    Sagar B Kudchodkar
    Department of Cancer Biology, Abramson Family Cancer Research Institute, 421 Curie Blvd, University of Pennsylvania, Philadelphia, PA 19104 6142, USA
    J Virol 78:11030-9. 2004
    ..Thus, HCMV induces mechanisms to maintain the integrity of the eIF4F complex even when mTOR signaling is inhibited...
  9. pmc The TORrid affairs of viruses: effects of mammalian DNA viruses on the PI3K-Akt-mTOR signalling pathway
    Nicholas J Buchkovich
    Department of Cancer Biology and Abramson Family Cancer Research Institute, University of Pennsylvania, 314 Biomedical Research Building, 421 Curie Blvd, Philadelphia, 19104 6142 Pennsylvania, USA
    Nat Rev Microbiol 6:266-75. 2008
    ..This Review will discuss the range of mechanisms that mammalian DNA viruses use to activate this pathway, as well as the multiple mechanisms these viruses have evolved to circumvent inhibitory stress signalling...
  10. pmc Human cytomegalovirus specifically controls the levels of the endoplasmic reticulum chaperone BiP/GRP78, which is required for virion assembly
    Nicholas J Buchkovich
    Department of Cancer Biology, 314 Biomedical Research Building, 421 Curie Blvd, School of Medicine, University of Pennsylvania, Philadelphia, PA 19104 6142, USA
    J Virol 82:31-9. 2008
    ..These data suggest that one effect of the controlled expression of BiP/GRP78 in infected cells is to aid in cytoplasmic virion assembly and egress...
  11. pmc AMPK-mediated inhibition of mTOR kinase is circumvented during immediate-early times of human cytomegalovirus infection
    Sagar B Kudchodkar
    Department of Cancer Biology, Abramson Family Cancer Research Institute, School of Medicine, University of Pennsylvania, 421 Curie Blvd, Philadelphia, PA 19104 6142, USA
    J Virol 81:3649-51. 2007
    ..However, growth curves showed that progeny virion production is inhibited when AICAR is added, suggesting other inhibitory effects of AICAR or activated AMPK...
  12. pmc Human cytomegalovirus infection alters the substrate specificities and rapamycin sensitivities of raptor- and rictor-containing complexes
    Sagar B Kudchodkar
    Department of Cancer Biology, Abramson Family Cancer Research Institute, School of Medicine, University of Pennsylvania, Philadelphia, PA 19104 6142, USA
    Proc Natl Acad Sci U S A 103:14182-7. 2006
    ..Our data also suggest that the present understanding of rapamycin's inhibitory effects is incomplete...
  13. pmc Human cytomegalovirus major immediate-early proteins and simian virus 40 large T antigen can inhibit apoptosis through activation of the phosphatidylinositide 3'-OH kinase pathway and the cellular kinase Akt
    Yongjun Yu
    Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 6142, USA
    J Virol 76:3731-8. 2002
    ..Transfection of plasmids which express the individual 86-kDa (IEP86; IE2(579aa)) and 72-kDa (IEP72; IE1(491aa)) MIEPs indicate that each MIEP could inhibit apoptosis via activation of the PI3 kinase pathway...
  14. pmc Strain variations in single amino acids of the 86-kilodalton human cytomegalovirus major immediate-early protein (IE2) affect its functional and biochemical properties: implications of dynamic protein conformation
    M Inmaculada Barrasa
    Department of Cancer Biology, Abramson Family Cancer Research Institute, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104 6142, USA
    J Virol 77:4760-72. 2003
    ..A hydrophobic core containing A463 is predicted to be responsible for the functional integrity of the carboxy-terminal region of IEP86 between amino acids 344 and 579...
  15. pmc Analysis of splice variants of the immediate-early 1 region of human cytomegalovirus
    Sita Awasthi
    Department of Cancer Biology, School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    J Virol 78:8191-200. 2004
    ..5 from its respective cDNA results in repression of viral gene expression in infected cells. We speculate that expression of these proteins during infection may be restricted to specific conditions or cell types...
  16. pmc The phosphorylation status of the serine-rich region of the human cytomegalovirus 86-kilodalton major immediate-early protein IE2/IEP86 affects temporal viral gene expression
    M Inmaculada Barrasa
    Department of Cancer Biology, Abramson Family Cancer Research Institute, School of Medicine, University of Pennsylvania, Philadelphia, PA 19104 6142, USA
    J Virol 79:1428-37. 2005
    ..The data show that the phosphorylation state of the serine-rich region, particularly between amino acids 271 and 275, modulates the temporal expression of viral genes...
  17. pmc Effects of simian virus 40 large and small tumor antigens on mammalian target of rapamycin signaling: small tumor antigen mediates hypophosphorylation of eIF4E-binding protein 1 late in infection
    Yongjun Yu
    Department of Cancer Biology, Abramson Family Cancer Research Institute, University of Pennsylvania, Philadelphia, PA 19104 6142, USA
    J Virol 79:6882-9. 2005
    ....
  18. pmc Human cytomegalovirus infection activates and regulates the unfolded protein response
    Jennifer A Isler
    Department of Cancer Biology, Abramson Family Cancer Research Institute, University of Pennsylvania, Philadelphia, USA
    J Virol 79:6890-9. 2005
    ..These results suggest that, although HCMV infection induces the unfolded protein response, it modifies the outcome to benefit viral replication...
  19. pmc Production of infectious human cytomegalovirus virions is inhibited by drugs that disrupt calcium homeostasis in the endoplasmic reticulum
    Jennifer A Isler
    Department of Cancer Biology, Abramson Family Cancer Research Institute, School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    J Virol 79:15388-97. 2005
    ..This can be accounted for by both the retarded accumulation of late gene products and the drug-induced depletion of ER calcium, which disrupts critical cellular functions needed for maturation...
  20. pmc 19S late mRNAs of simian virus 40 have an internal ribosome entry site upstream of the virion structural protein 3 coding sequence
    Yongjun Yu
    Department of Cancer Biology, Abramson Family Cancer Research Institute, 314 Biomedical Research Building, 421 Curie Blvd, University of Pennsylvania, Philadelphia, PA 19104 6142, USA
    J Virol 80:6553-8. 2006
    ..These data strongly suggest that an IRES can be utilized for VP3 translation from the SV40 19S late mRNAs...
  21. pmc Characterization of a human cytomegalovirus with phosphorylation site mutations in the immediate-early 2 protein
    Julie A Heider
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544 1014, USA
    J Virol 76:928-32. 2002
    ..The modification of the IE2 protein by SUMO-1 (i.e., its sumoylated state) was also examined...

Research Grants45

  1. REGULATION OF CELLULAR FUNCTIONS BY DNA TUMOR VIRUSES
    JAMES ALWINE; Fiscal Year: 2009
    ..Aim 3a. Determine how t Ag is mediating the hypophosphorylation. Aim 3b. Detect and characterize IRESs in the SV40 late mRNAs. ..
  2. REGULATION OF DNA TUMOR VIRUS GENE EXPRESSION
    JAMES ALWINE; Fiscal Year: 2000
    ..In aim 4 the interaction between the viral activators and sequence-specific promoter factors will be characterized to determine how this may correlate with basal transcription complex interactions and activation. ..
  3. MECHANISMS OF POLYADENYLATION IN VIRAL SYSTEMS
    JAMES ALWINE; Fiscal Year: 2002
    ..abstract_text> ..
  4. REGULATION OF CELLULAR FUNCTIONS BY DNA TUMOR VIRUSES
    JAMES ALWINE; Fiscal Year: 2007
    ..Aim 3a. Determine how t Ag is mediating the hypophosphorylation. Aim 3b. Detect and characterize IRESs in the SV40 late mRNAs. ..
  5. MECHANISMS OF POLYADENYLATION IN VIRAL SYSTEMS
    JAMES ALWINE; Fiscal Year: 2007
    ..These analyses will open new lines of research to characterize the functional connections between the factors in the complex and decipher the mechanisms which control processing at the level of coupling. ..
  6. REGULATION OF CELLULAR FUNCTIONS BY DNA TUMOR VIRUSES
    JAMES ALWINE; Fiscal Year: 2005
    ..These studies will provide new insights into the mechanisms by which T Ag and the MIEPs manipulate cellular transcription, metabolism, the cell cycle and apoptosis. ..
  7. REGULATION OF DNA TUMOR VIRUS GENE EXPRESSION
    JAMES ALWINE; Fiscal Year: 1980
    ..This characterization will allow the studies to be extended to analysis of the molecular mechanism of this level of gene expression control...
  8. MECHANISMS OF POLYADENYLATION IN VIRAL SYSTEMS
    JAMES ALWINE; Fiscal Year: 1993
    ..We will test the efficiency of the HIV signal under conditions known to activate HIV gene expression and in the presence..
  9. REGULATION OF DNA TUMOR VIRUS GENE EXPRESSION
    JAMES ALWINE; Fiscal Year: 1993
    ..Therefore, we will utilize the genetics of yeast as an alternate system to determine the mechanisms by which T antigen affects transcriptional control and the cell cycle...
  10. REGULATION OF DNA TUMOR VIRUS GENE EXPRESSION
    JAMES ALWINE; Fiscal Year: 1991
    ..We propose to continue these studies in order to better understand the phenomenon and determine the function(s) of the agnoprotein...