Research Topics
Genomes and GenesSpecies | Waddah AlrefaiSummaryAffiliation: University of Illinois at Chicago Country: USA Publications
Research Grants
| Collaborators
|
Detail Information
Publications
D-Glucose modulates intestinal Niemann-Pick C1-like 1 (NPC1L1) gene expression via transcriptional regulationPooja Malhotra
Section of Digestive Diseases and Nutrition, Department of Medicine, University of Illinois at Chicago, and Jesse Brown VA Medical Center, Chicago, Illinois 60612, USA
Am J Physiol Gastrointest Liver Physiol 304:G203-10. 2013..In conclusion, glucose appears to directly modulate NPC1L1 expression via transcriptional mechanisms and the involvement of phosphatase-dependent pathways...- Enteropathogenic Escherichia coli inhibits ileal sodium-dependent bile acid transporter ASBTFadi Annaba
Section of Digestive Diseases and Nutrition, Department of Medicine, University of Illinois at Chicago, Chicago, IL 60612, USA
Am J Physiol Gastrointest Liver Physiol 302:G1216-22. 2012..Our studies provide novel evidence for the alterations in the activity of ASBT by EPEC infection and suggest a possible effect for EPEC in influencing intestinal bile acid homeostasis... 
Molecular cloning and promoter analysis of downregulated in adenoma (DRA)Waddah A Alrefai
University of Illinois at Chicago, Jesse Brown VA Medical Center, Medical Research Service, Chicago, IL 60612, USA
Am J Physiol Gastrointest Liver Physiol 293:G923-34. 2007..Our studies provide evidence for the involvement of HNF-4, YY1, and GATA transcription factors in DRA expression in intestinal differentiated epithelial cells...- Bile acid transporters: structure, function, regulation and pathophysiological implicationsWaddah A Alrefai
Section of Digestive Diseases and Nutrition, Department of Medicine, University of Illinois at Chicago, Chicago, Illinois 60612, USA
Pharm Res 24:1803-23. 2007..This review will highlight current knowledge about structure, function and molecular characterization of bile acid transporters and discuss the implications of their defects in various hepatic and intestinal disorders... - Modulation of human Niemann-Pick C1-like 1 gene expression by sterol: Role of sterol regulatory element binding protein 2Waddah A Alrefai
Section of Digestive Diseases and Nutrition, Department of Medicine, University of Illinois, and Jesse Brown Veterans Affairs Medical Center, Chicago, IL 60612, USA
Am J Physiol Gastrointest Liver Physiol 292:G369-76. 2007..Our study demonstrated the modulation of human NPC1L1 expression and promoter activity by cholesterol in a SREBP-2-dependent mechanism... - Cholesterol modulates human intestinal sodium-dependent bile acid transporterWaddah A Alrefai
Section of Digestive Diseases and Nutrition, Department of Medicine, University of Illinois at Chicago and Jesse Brown Veteran Affairs Medical Center, Medical Research Service 600 151 820 South Damen Ave, Chicago, Illinois 60612, USA
Am J Physiol Gastrointest Liver Physiol 288:G978-85. 2005..These data provide novel evidence for the direct regulation of human ASBT function by cholesterol and suggest that this phenomenon may play a central role in cholesterol homeostasis... - Modulation of ileal apical Na+-dependent bile acid transporter ASBT by protein kinase CZaheer Sarwar
Section of Digestive Diseases and Nutrition, Department of Medicine, University of Illinois at Chicago and Jesse Brown Veterans Affairs Medical Center, Chicago, Illinios 60612, USA
Am J Physiol Gastrointest Liver Physiol 297:G532-8. 2009..Our novel findings demonstrate a posttranscriptional modulation of ileal ASBT function and membrane expression by phorbol ester via a PKCzeta-dependent pathway... - Modulation of ileal bile acid transporter (ASBT) activity by depletion of plasma membrane cholesterol: association with lipid raftsFadi Annaba
Section of Digestive Diseases and Nutrition, Department of Medicine, University of Illinois at Chicago, IL, USA
Am J Physiol Gastrointest Liver Physiol 294:G489-97. 2008.... - Taurodeoxycholate modulates apical Cl-/OH- exchange activity in Caco2 cellsWaddah A Alrefai
Section of Digestive Diseases and Nutrition, Department of Medicine, University of Illinois at Chicago, and Jesse Brown VA Medical Center, Chicago, Ilinois 60612, USA
Dig Dis Sci 52:1270-8. 2007..In conclusion, our studies provide direct evidence for inhibition of human intestinal apical Cl(-)/OH(-) exchange activity by bile acids via Ca(2+)-, PI3 kinase-, and PKC beta I-dependent pathways in Caco2 cells... - Green tea catechin EGCG inhibits ileal apical sodium bile acid transporter ASBTFadi Annaba
Department of Medicine, University of Illinois at Chicago, and Jesse Brown Veterans Affairs Medical Center, USA
Am J Physiol Gastrointest Liver Physiol 298:G467-73. 2010..In conclusion, our data demonstrate a novel role of lipid rafts in the modulation of ASBT function by the dietary component EGCG, which may underlie the hypocholesterolemic effects of green tea... - Regulation of monocarboxylate transporter 1 (MCT1) promoter by butyrate in human intestinal epithelial cells: involvement of NF-kappaB pathwayAlip Borthakur
Section of Digestive Diseases and Nutrition, Department of Medicine, University of Illinois at Chicago and Jesse Brown VA Medical Center, Chicago, Illinois 60612, USA
J Cell Biochem 103:1452-63. 2008..Our results demonstrate the involvement of NF-kappaB pathway in the regulation of MCT1 promoter activity by butyrate... - Mechanisms of lysophosphatidic acid (LPA) mediated stimulation of intestinal apical Cl-/OH- exchangeAmika Singla
Section of Digestive Diseases and Nutrition, Department of Physiology, University of Illinois at Chicago, Chicago, IL 60612, USA
Am J Physiol Gastrointest Liver Physiol 298:G182-9. 2010..Our results demonstrate that LPA stimulates apical Cl(-)/OH(-) exchange activity and surface levels of DRA in intestinal epithelial cells. This increase in Cl(-)/OH(-) exchange may contribute to the antidiarrheal effects of LPA... - Involvement of c-Src and protein kinase C delta in the inhibition of Cl(-)/OH- exchange activity in Caco-2 cells by serotoninSeema Saksena
Section of Digestive Diseases and Nutrition, Department of Medicine, University of Illinois and Jesse Brown Veterans Affairs Medical Center, Chicago, Illinois 60612, USA
J Biol Chem 280:11859-68. 2005..viii) 5-HT stimulated tyrosine phosphorylation of c-Src kinase and PKC delta... - Lactobacillus acidophilus stimulates the expression of SLC26A3 via a transcriptional mechanismGeetu Raheja
Department of Medicine, University of Illinois at Chicago and Jesse Brown Veterans Affairs Medical Center, 60612, USA
Am J Physiol Gastrointest Liver Physiol 298:G395-401. 2010..In conclusion, increase in DRA promoter activity and expression may contribute to the upregulation of intestinal electrolyte absorption and might underlie the potential antidiarrheal effects of LA... 
Enteropathogenic Escherichia coli infection inhibits intestinal serotonin transporter function and expressionAli Esmaili
Section of Digestive Diseases and Nutrition, University of Illinois at Chicago, Chicago, Illinois 60612, USA
Gastroenterology 137:2074-83. 2009..The present studies examined the effects of EPEC infection on SERT activity and expression in intestinal epithelial cells and elucidated the underlying mechanisms...- Serotonin inhibits Na+/H+ exchange activity via 5-HT4 receptors and activation of PKC alpha in human intestinal epithelial cellsRavinder K Gill
Section of Digestive Diseases and Nutrition, University of Illinois at Chicago, Jesse Brown VA Medical Center, IL 60612, USA
Gastroenterology 128:962-74. 2005..The present studies examined the acute effects of 5-hydroxytryptamine (5-HT) on NHE activity using Caco-2 cells as an in vitro model... - Ileal apical Na+-dependent bile acid transporter ASBT is upregulated in rats with diabetes mellitus induced by low doses of streptozotocinFadi Annaba
Department of Medicine, University of Illinois at Chicago, USA
Am J Physiol Gastrointest Liver Physiol 299:G898-906. 2010..The increase in ASBT expression may contribute to disturbances in cholesterol homeostasis associated with diabetes mellitus... - The probiotic Lactobacillus acidophilus stimulates chloride/hydroxyl exchange activity in human intestinal epithelial cellsAlip Borthakur
Section of Digestive Diseases and Nutrition, Department of Medicine, University of Illinois at Chicago, and Jesse Brown Veterans Affairs Medical Center, Chicago, IL 60612, USA
J Nutr 138:1355-9. 2008..Our results suggest that LA secretes soluble effector molecule(s) into the culture medium that stimulate apical Cl(-)/OH(-) exchange activity via phosphatidylinositol-3 kinase mediated mechanism... - Mechanisms underlying modulation of monocarboxylate transporter 1 (MCT1) by somatostatin in human intestinal epithelial cellsSeema Saksena
Section of Digestive Diseases and Nutrition, Department of Medicine, University of Illinois at Chicago and Jesse Brown Veterans Affairs Medical Center, Chicago, Illinois 60612, USA
Am J Physiol Gastrointest Liver Physiol 297:G878-85. 2009..Our findings demonstrate that increased MCT1 association with CD147 at the apical membrane in response to SST is p38 MAP kinase dependent and underlies the stimulatory effects of SST on butyrate uptake... - Mechanisms of transcriptional modulation of the human anion exchanger SLC26A3 gene expression by IFN-{gamma}Seema Saksena
Section of Digestive Diseases and Nutrition, Department of Medicine, University of Illinois at Chicago, Chicago, IL 60612, USA
Am J Physiol Gastrointest Liver Physiol 298:G159-66. 2010..Mutations in the potential GAS element abrogated the inhibitory effects of IFN-gamma. These studies provide evidence for the involvement of STAT1 in the inhibition of SLC26A3 gene expression by IFN-gamma in the human intestine... - Platelet-activating factor-induced NF-kappaB activation and IL-8 production in intestinal epithelial cells are Bcl10-dependentAlip Borthakur
Section of Digestive Diseases and Nutrition, Department of Medicine, University of Illinois at Chicago and Jesse Brown VA Medical Center, Chicago, Illinois 60612, USA
Inflamm Bowel Dis 16:593-603. 2010..However, its mechanisms of inducing inflammatory responses are not fully understood. Therefore, studies were designed to explore the mechanisms of PAF-induced inflammatory cascade in intestinal epithelial cells... - PKC-dependent stimulation of the human MCT1 promoter involves transcription factor AP2Seema Saksena
Department of Medicine, University of Illinois at Chicago, Chicago, IL 60612, USA
Am J Physiol Gastrointest Liver Physiol 296:G275-83. 2009..These findings showing the regulation of MCT1 promoter by PKC and AP2 are of significant importance for an understanding of the molecular regulation of SCFA absorption in the human intestine... - Epidermal growth factor upregulates serotonin transporter in human intestinal epithelial cells via transcriptional mechanismsRavinder K Gill
Section of Digestive Diseases and Nutrition, Department of Medicine, University of Illinois at Chicago and Jesse Brown VA Medical Center, 60612, USA
Am J Physiol Gastrointest Liver Physiol 300:G627-36. 2011..Our findings define novel mechanisms of transcriptional regulation of SERT by EGF via EGFR at the promoter level that may contribute to the beneficial effects of EGF in gut disorders... - Characterization of the 5'-flanking region and regulation of expression of human anion exchanger SLC26A6Seema Saksena
Department of Medicine, University of Illinois at Chicago, Jesse Brown VA Medical Center, Chicago, Illinois 60612, USA
J Cell Biochem 105:454-66. 2008..Mutations in the potential ISRE site abrogated the inhibitory effects of IFNgamma. These studies provided novel evidence for the involvement of IRF-1 in the regulation of SLC26A6 gene expression by IFNgamma in the human intestine... - Serotonin modifies cytoskeleton and brush-border membrane architecture in human intestinal epithelial cellsRavinder K Gill
Dept of Medicine, Univ of Illinois at Chicago, Medical Research Service, Jesse Brown VA Medical Center, 820 S Damen Ave, Chicago, IL 60612, USA
Am J Physiol Gastrointest Liver Physiol 295:G700-8. 2008.... - Function, expression, and characterization of the serotonin transporter in the native human intestineRavinder K Gill
Research Assistant Professor, Univ of Illinois at Chicago, Jesse Brown V A Medical Center, Medical Research Service 600 151 820 South Damen Ave, Chicago, IL 60612
Am J Physiol Gastrointest Liver Physiol 294:G254-62. 2008.... - Expression and membrane localization of MCT isoforms along the length of the human intestineRavinder K Gill
Section of Digestive Diseases and Nutrition, Department of Medicine, University of Illinois at Chicago, Medical Research Service 600 151, Jesse Brown Veterans Affairs Medical Center, 820 South Damen Ave, Chicago, Illinois 60612, USA
Am J Physiol Cell Physiol 289:C846-52. 2005..We speculate that distinct MCT isoforms may be involved in SCFA transport across the apical or basolateral membranes in polarized colonic epithelial cells... - Regulation of NHE3 by nitric oxide in Caco-2 cellsRavinder K Gill
Section of Digestive and Liver Diseases, Department of Medicine, University of Illinois at Chicago and West Side Veterans Affairs Medical Center, Chicago, Illinois 60612, USA
Am J Physiol Gastrointest Liver Physiol 283:G747-56. 2002..These data provide novel evidence that NO inhibits NHE3 activity via activation of soluble guanylate cyclase, resulting in an increase in intracellular cGMP levels and activation of protein kinase G... - Stimulation of apical Cl⁻/HCO₃⁻(OH⁻) exchanger, SLC26A3 by neuropeptide Y is lipid raft dependentSeema Saksena
Dept of Medicine, University of Illinois at Chicago and Jesse Brown Veterans Affairs Medical Center, Illinois 60612, USA
Am J Physiol Gastrointest Liver Physiol 299:G1334-43. 2010..Our findings suggest that the alteration in the association of DRA with lipid rafts may contribute to the proabsorptive effects of NPY in the human intestine... - Regulation of intestinal serotonin transporter expression via epigenetic mechanisms: role of HDAC2Ravinder K Gill
Dept of Medicine, Univ of Illinois at Chicago, Jesse Brown VA Medical Center, 820 South Damen Ave, Chicago, IL 60612 E mail
Am J Physiol Cell Physiol 304:C334-41. 2013..Our results identify a novel role of HDAC2 as a regulator of SERT gene expression in intestinal epithelial cells... - Role of Fyn and PI3K in H2O2-induced inhibition of apical Cl-/OH- exchange activity in human intestinal epithelial cellsSeema Saksena
Department of Medicine, University of Illinois at Chicago, Jesse Brown VA Medical Center, Chicago, IL 60612, USA
Biochem J 416:99-108. 2008.... - Inhibition of apical Cl-/OH- exchange activity in Caco-2 cells by phorbol esters is mediated by PKCepsilonSeema Saksena
Section of Digestive and Liver Diseases, Department of Medicine, University of Illinois at Chicago and West Side Department of Veterans Affairs Medical Center, Chicago, Illinois 60612, USA
Am J Physiol Cell Physiol 283:C1492-500. 2002.... - Modulation of Cl-/OH- exchange activity in Caco-2 cells by nitric oxideSeema Saksena
Section of Digestive and Liver Diseases, Department of Medicine, University of Illinois at Chicago and Chicago Veteran's Affairs System: West Side Division, Chicago, Illinois 60612, USA
Am J Physiol Gastrointest Liver Physiol 283:G626-33. 2002..Our results suggest that NO-induced inhibition of Cl-/OH- exchange may play an important role in the pathophysiology of diarrhea associated with inflammatory bowel diseases... - SREBP2 mediates the modulation of intestinal NPC1L1 expression by curcuminPradeep Kumar
Section of Digestive Diseases and Nutrition, Department of Medicine, University of Illinois at Chicago, USA
Am J Physiol Gastrointest Liver Physiol 301:G148-55. 2011..Our studies demonstrate that curcumin directly modulates intestinal NPC1L1 expression via transcriptional regulation and the involvement of SREBP2 transcription factor...
Research Grants
- Regulation of Intestinal Bile Acid TransportWaddah Alrefai; Fiscal Year: 2009..A better understanding of the role of bile acid absorption in cholesterol related disorders may help advance new therapeutic modalities to counter diseases such as hypercholesterolemia and atherosclerosis. ..
- Regulation of Intestinal Bile Acid TransportWaddah Alrefai; Fiscal Year: 2007..A better understanding of the role of bile acid absorption in cholesterol related disorders may help advance new therapeutic modalities to counter diseases such as hypercholesterolemia and atherosclerosis. ..