Research Topics
| Graca Almeida-PoradaSummaryAffiliation: University of Nevada Country: USA Publications
Research Grants
| Collaborators
|
Detail Information
Publications
Formation of human hepatocytes by human hematopoietic stem cells in sheepGraca Almeida-Porada
Department of Animal Biotechnology, University of Nevada Reno, Mail Stop 202, Reno, NV 89557 0104, USA
Blood 104:2582-90. 2004....- Plasticity of human stem cells in the fetal sheep model of human stem cell transplantationGraca Almeida-Porada
Department of Animal Biotechnology, University of Nevada, Reno, Nevada 89557 0104, USA
Int J Hematol 79:1-6. 2004..In this review, we describe the advantages of the permissive aspects of the developing preimmune fetus in the early gestational age that led us to develop the sheep as a large-animal model of human stem cell plasticity... - In vivo haematopoietic potential of human neural stem cellsG Almeida-Porada
Department of Animal Biotechnology, School of Veterinary Medicine and Department of Medicine, University of Nevada, Reno and VA Medical Center, Reno, NV, USA
Br J Haematol 130:276-83. 2005.... 
The human-sheep chimeras as a model for human stem cell mobilization and evaluation of hematopoietic grafts' potentialGraca Almeida-Porada
Department of Animal Biotechnology University of Nevada, Reno, NV 89557 0104, USA
Exp Hematol 35:1594-600. 2007..To investigate whether the sheep xenograft model of human hematopoiesis can be used to mimic mobilization of human hematopoietic stem cells in vivo...- Bone marrow stem cells and liver regenerationGraca Almeida-Porada
Department of Animal Biotechnology, University of Nevada, Reno, Reno, NV 89557 0104, USA
Exp Hematol 38:574-80. 2010..Here, we will summarize the ability of each of these stem cell populations to give rise to functional hepatic elements that could mediate repair in patients with liver damage/disease... - A human stromal-based serum-free culture system supports the ex vivo expansion/maintenance of bone marrow and cord blood hematopoietic stem/progenitor cellsCláudia Lobato da Silva
Department of Animal Biotechnology, University of Nevada, Reno, 89557, USA
Exp Hematol 33:828-35. 2005..We investigated the role of human stromal layers (hu-ST) on the ex vivo expansion/maintenance of human hematopoietic stem/progenitor cells (HSC) from adult bone marrow (BM) and umbilical cord blood (CB)... - Human mesenchymal stem cells form Purkinje fibers in fetal sheep heartJudith A Airey
Department of Pharmacology, MS 318, University of Nevada, Reno, Reno, NV 89523, USA
Circulation 109:1401-7. 2004..We have investigated the usefulness of a model of cardiac development in a large mammal, sheep, for studies of engraftment of human stem cells in the heart... - A Stro-1(+) human universal stromal feeder layer to expand/maintain human bone marrow hematopoietic stem/progenitor cells in a serum-free culture systemRaquel Gonçalves
Department of Animal Biotechnology, University of Nevada, Reno, NV 89557, USA
Exp Hematol 34:1353-9. 2006..Furthermore, we compared the results obtained with MSC as a feeder layer to traditional allogeneic stromal layers grown in long-term bone marrow culture media (LT-ST)... 
Differences amid bone marrow and cord blood hematopoietic stem/progenitor cell division kineticsCláudia Lobato da Silva
Department of Animal Biotechnology, University of Nevada, Reno, Nevada 89557 0104, USA
J Cell Physiol 220:102-11. 2009....- Human natural killer cell development in a xenogeneic culture systemIsabel Barao
University of Nevada School of Medicine, V A Medical Center, Reno, USA
Br J Haematol 118:885-92. 2002.... - Differentiative potential of human metanephric mesenchymal cellsGraca Almeida-Porada
Department of Animal Biotechnology, University of Nevada Reno, Mail Stop 202, Reno, NV 89557 0104, USA
Exp Hematol 30:1454-62. 2002..To evaluate the ability of mesenchymal cells derived from nonhematopoietic organs to form blood and other tissues in vitro and in vivo... - Persistent circulating human insulin in sheep transplanted in utero with human mesenchymal stem cellsAdel Ersek
Department of Animal Biotechnology, University of Nevada Reno, Reno, NV, USA
Exp Hematol 38:311-20. 2010..To determine if mesenchymal stem cells (MSC) derived from human fetal pancreatic tissue (pMSC) would engraft and differentiate in sheep pancreas following transplantation in utero... - Expression levels of the PiT-2 receptor explain, in part, the gestational age-dependent alterations in transduction efficiency after in utero retroviral-mediated gene transferFerhat Ozturk
Department of Animal Biotechnology, University of Nevada, Reno, NV, USA
J Gene Med 14:169-81. 2012.... - Phenotypic correction of hemophilia A in sheep by postnatal intraperitoneal transplantation of FVIII-expressing MSCChristopher D Porada
Wake Forest Institute for Regenerative Medicine, Winston Salem, NC, USA
Exp Hematol 39:1124-1135.e4. 2011..In summary, this novel, nonablative MSC transplantation was straightforward, safe, and converted life-threatening, debilitating HA to a moderate phenotype in a large animal model... - Male germ-line cells are at risk following direct-injection retroviral-mediated gene transfer in uteroChristopher D Porada
Department of Animal Biotechnology, University of Nevada at Reno, 89557 0104, USA
Mol Ther 12:754-62. 2005..In conclusion, our analysis on over 3 x 10(9) sperm cells suggests that the direct-injection approach employed in these studies may result in the inadvertent transduction of very low numbers of male germ cells... - Modulation of human mesenchymal stem cell immunogenicity through forced expression of human cytomegalovirus us proteinsMelisa A Soland
Department of Animal Biotechnology, University of Nevada, Reno, Nevada, United States of America
PLoS ONE 7:e36163. 2012..Human cytomegalovirus (HCMV) has developed several strategies to evade cytotoxic T lymphocyte (CTL) and Natural Killer (NK) cell recognition. Our goal is to exploit HCMV immunological evasion strategies to reduce MSC immunogenicity... - A large animal noninjury model for study of human stem cell plasticityGraca Almeida-Porada
Department of Animal Biotechnology, University of Nevada, Reno, NV 89557 0104, USA
Blood Cells Mol Dis 32:77-81. 2004.... - The sheep model of in utero gene therapyChristopher D Porada
Department of Animal Biotechnology, University of Nevada, Reno, Nev 89557 0104, USA
Fetal Diagn Ther 19:23-30. 2004.... - Factors determining the risk of inadvertent retroviral transduction of male germ cells after in utero gene transfer in sheepPaul J Park
Department of Animal Biotechnology, School of Veterinary Medicine, University of Nevada, Reno, NV 89557, USA
Hum Gene Ther 20:201-15. 2009.... - Reversible expression of CD34 by adult human bone marrow long-term engrafting hematopoietic stem cellsEsmail D Zanjani
Department of Animal Biotechnology, University of Nevada, Reno, Nev, USA
Exp Hematol 31:406-12. 2003.... - Human haematopoietic stem cells that mediate long-term in vivo engraftment are not susceptible to infection by human cytomegalovirusKirsten B Crapnell
Department of Veterans Affairs Medical Center, University of Nevada Reno, Reno, NV, USA
Br J Haematol 124:676-84. 2004..These results provide evidence that the long-term engrafting HSC is not a primary target of HCMV and suggest that HCMV infection of human haematopoietic cells is exercised at the level of committed progenitors... - Development and characterization of a novel CD34 monoclonal antibody that identifies sheep hematopoietic stem/progenitor cellsChristopher D Porada
Department of Animal Biotechnology, School of Veterinary Medicine, University of Nevada, Reno, NV 89557 0104, USA
Exp Hematol 36:1739-49. 2008..The current studies were undertaken to create a monoclonal antibody to sheep CD34 that would enable isolation and study of sheep HSC/progenitors... - Mesenchymal stem cells as therapeutics and vehicles for gene and drug deliveryChristopher D Porada
Department of Animal Biotechnology, University of Nevada, Reno, 89557 0104, USA
Adv Drug Deliv Rev 62:1156-66. 2010.... - Mesenchymal stem cells contribute to endogenous FVIII:c productionChad Sanada
Department of Animal Biotechnology, University of Nevada, Reno, NV, USA
J Cell Physiol 228:1010-6. 2013..These results demonstrate that MSC can contribute at low levels to the functional FVIII pool, and advance the understanding of the physiology of FVIII production and secretion... - Distinct contribution of human cord blood-derived endothelial colony forming cells to liver and gut in a fetal sheep modelJoshua A Wood
Department of Animal Biotechnology, University of Nevada, Reno, NV, USA
Hepatology 56:1086-96. 2012.... - Generation of tissue-specific cells from MSC does not require fusion or donor-to-host mitochondrial/membrane transferEvan J Colletti
Department of Animal Biotechnology, University of Nevada at Reno, Reno, NV 89557, USA
Stem Cell Res 2:125-38. 2009.... - In vivo generation of beta-cell-like cells from CD34(+) cells differentiated from human embryonic stem cellsA Daisy Goodrich
Department of Animal Biotechnology, University of Nevada, Reno, Reno, NV 89557, USA
Exp Hematol 38:516-525.e4. 2010..Based on this observation, we explored the capacity of CD34(+) cells derived in culture from the differentiation of human embryonic stem cells (hESC), for their in vivo pancreatic endocrine capacity... - Reduced stem cell factor links smooth myopathy and loss of interstitial cells of cajal in murine diabetic gastroparesisViktor J Horváth
Department of Physiology and Cell Biology, University of Nevada, Reno, Nevada, USA
Gastroenterology 130:759-70. 2006..Most murine gastric ICC depend on stem cell factor (SCF) signaling but can also be maintained with insulin or insulin-like growth factor-I (IGF-I). We investigated whether SCF could mediate the actions of insulin and IGF-I... - Generation of functional natural killer and dendritic cells in a human stromal-based serum-free culture system designed for cord blood expansionAna M Frias
Department of Animal Biotechnology, University of Nevada, Reno 89557 0104, USA
Exp Hematol 36:61-8. 2008..In this study, we investigated the ability of the CD7(+)CD34(-) population to differentiate into natural killer and dendritic cells (DCs)... - Purification of interstitial cells of Cajal by fluorescence-activated cell sortingTAMAS ORDOG
Department of Physiology and Cell Biology, University of Nevada, Reno School of Medicine, Anderson Bldg, Mail Stop 352, Reno, NV 89557, USA
Am J Physiol Cell Physiol 286:C448-56. 2004..Smooth muscle cells and myenteric neurons were also depleted. We conclude that immunofluorescence-based sorting can yield ICC in sufficiently high numbers and purity to permit detailed molecular analyses... - Early fetal gene delivery utilizes both central and peripheral mechanisms of tolerance inductionEvan Colletti
Department of Animal Biotechnology, University of Nevada, Reno, NV 89557 0104, USA
Exp Hematol 36:816-22. 2008..In the present studies, we conduct detailed analysis of the thymus of recipients of in utero gene transfer (IUGT) to delineate the mechanism of the observed immune tolerance and assess the impact of recipient age on this process... - Docosahexaenoic acid induces dose dependent cell death in an early undifferentiated subtype of acute myeloid leukemia cell lineTakashi Yamagami
Department of Animal Biotechnology, University of Nevada, Reno, NV 89557 0104, USA
Cancer Biol Ther 8:331-7. 2009..Since we also show that DHA does not have a detrimental effect on normal hematopoiesis our results suggest that DHA could potentially serve as an well-tolerated adjuvant in the treatment of AML patients... - Efficient generation of human hepatocytes by the intrahepatic delivery of clonal human mesenchymal stem cells in fetal sheepJason Chamberlain
Department of Animal Biotechnology, University of Nevada at Reno, Reno, NV 89557 0104, USA
Hepatology 46:1935-45. 2007.... - HCMV protein LUNA is required for viral reactivation from latently infected primary CD14⁺ cellsLisa R Keyes
Department of Microbiology and Graduate Program in Cell and Molecular Biology, University of Nevada, Reno, Nevada, United States of America
PLoS ONE 7:e52827. 2012....
Research Grants
- Functional Analysis of the Hematopoietic Niche during OntogenyGraca Almeida Porada; Fiscal Year: 2009..This would significantly improve the safety and success of clinical HSC transplantation and potentially allow its application to a wider range of diseases affecting human patients. ..
- In Utero Stem Cell Transplantation for Hemophilia AGraca Almeida Porada; Fiscal Year: 2006..abstract_text> ..
- Evaluation of Stem Cell Plasticity In VivoGraca Almeida Porada; Fiscal Year: 2004..With these studies we hope to provide novel insights into the location of latent stem cell poois within the body and define the behavior and capabilities of these cells that are potentially of great clinical value. ..
- Functional Analysis of the Hematopoietic Niche during OntogenyGRACA DUARTE ALMEIDA PORADA; Fiscal Year: 2010..This would significantly improve the safety and success of clinical HSC transplantation and potentially allow its application to a wider range of diseases affecting human patients. ..