Graca Almeida-Porada

Summary

Affiliation: University of Nevada
Country: USA

Publications

  1. pmc The human-sheep chimeras as a model for human stem cell mobilization and evaluation of hematopoietic grafts' potential
    Graca Almeida-Porada
    Department of Animal Biotechnology University of Nevada, Reno, NV 89557 0104, USA
    Exp Hematol 35:1594-600. 2007
  2. pmc Bone marrow stem cells and liver regeneration
    Graca Almeida-Porada
    Department of Animal Biotechnology, University of Nevada, Reno, Reno, NV 89557 0104, USA
    Exp Hematol 38:574-80. 2010
  3. ncbi request reprint Plasticity of human stem cells in the fetal sheep model of human stem cell transplantation
    Graca Almeida-Porada
    Department of Animal Biotechnology, University of Nevada, Reno, Nevada 89557 0104, USA
    Int J Hematol 79:1-6. 2004
  4. ncbi request reprint Formation of human hepatocytes by human hematopoietic stem cells in sheep
    Graca Almeida-Porada
    Department of Animal Biotechnology, University of Nevada Reno, Mail Stop 202, Reno, NV 89557 0104, USA
    Blood 104:2582-90. 2004
  5. ncbi request reprint In vivo haematopoietic potential of human neural stem cells
    G Almeida-Porada
    Department of Animal Biotechnology, School of Veterinary Medicine and Department of Medicine, University of Nevada, Reno and VA Medical Center, Reno, NV, USA
    Br J Haematol 130:276-83. 2005
  6. ncbi request reprint Human mesenchymal stem cells form Purkinje fibers in fetal sheep heart
    Judith A Airey
    Department of Pharmacology, MS 318, University of Nevada, Reno, Reno, NV 89523, USA
    Circulation 109:1401-7. 2004
  7. ncbi request reprint Gestational age of recipient determines pattern and level of transgene expression following in utero retroviral gene transfer
    Christopher D Porada
    Department of Animal Biotechnology, University of Nevada at Reno, Reno, NV 89557, USA
    Mol Ther 11:284-93. 2005
  8. ncbi request reprint A human stromal-based serum-free culture system supports the ex vivo expansion/maintenance of bone marrow and cord blood hematopoietic stem/progenitor cells
    Cláudia Lobato da Silva
    Department of Animal Biotechnology, University of Nevada, Reno, 89557, USA
    Exp Hematol 33:828-35. 2005
  9. doi request reprint Differences amid bone marrow and cord blood hematopoietic stem/progenitor cell division kinetics
    Cláudia Lobato da Silva
    Department of Animal Biotechnology, University of Nevada, Reno, Nevada 89557 0104, USA
    J Cell Physiol 220:102-11. 2009
  10. ncbi request reprint Transduction of long-term-engrafting human hematopoietic stem cells by retroviral vectors
    Christopher D Porada
    Department of Animal Biotechnology, University of Nevada, Reno, NV 89520, USA
    Hum Gene Ther 13:867-79. 2002

Research Grants

  1. Functional Analysis of the Hematopoietic Niche during Ontogeny
    Graca Almeida Porada; Fiscal Year: 2009
  2. In Utero Stem Cell Transplantation for Hemophilia A
    Graca Almeida Porada; Fiscal Year: 2006
  3. Evaluation of Stem Cell Plasticity In Vivo
    Graca Almeida Porada; Fiscal Year: 2004
  4. Functional Analysis of the Hematopoietic Niche during Ontogeny
    GRACA DUARTE ALMEIDA PORADA; Fiscal Year: 2010

Collaborators

Detail Information

Publications38

  1. pmc The human-sheep chimeras as a model for human stem cell mobilization and evaluation of hematopoietic grafts' potential
    Graca Almeida-Porada
    Department of Animal Biotechnology University of Nevada, Reno, NV 89557 0104, USA
    Exp Hematol 35:1594-600. 2007
    ..To investigate whether the sheep xenograft model of human hematopoiesis can be used to mimic mobilization of human hematopoietic stem cells in vivo...
  2. pmc Bone marrow stem cells and liver regeneration
    Graca Almeida-Porada
    Department of Animal Biotechnology, University of Nevada, Reno, Reno, NV 89557 0104, USA
    Exp Hematol 38:574-80. 2010
    ..Here, we will summarize the ability of each of these stem cell populations to give rise to functional hepatic elements that could mediate repair in patients with liver damage/disease...
  3. ncbi request reprint Plasticity of human stem cells in the fetal sheep model of human stem cell transplantation
    Graca Almeida-Porada
    Department of Animal Biotechnology, University of Nevada, Reno, Nevada 89557 0104, USA
    Int J Hematol 79:1-6. 2004
    ..In this review, we describe the advantages of the permissive aspects of the developing preimmune fetus in the early gestational age that led us to develop the sheep as a large-animal model of human stem cell plasticity...
  4. ncbi request reprint Formation of human hepatocytes by human hematopoietic stem cells in sheep
    Graca Almeida-Porada
    Department of Animal Biotechnology, University of Nevada Reno, Mail Stop 202, Reno, NV 89557 0104, USA
    Blood 104:2582-90. 2004
    ....
  5. ncbi request reprint In vivo haematopoietic potential of human neural stem cells
    G Almeida-Porada
    Department of Animal Biotechnology, School of Veterinary Medicine and Department of Medicine, University of Nevada, Reno and VA Medical Center, Reno, NV, USA
    Br J Haematol 130:276-83. 2005
    ....
  6. ncbi request reprint Human mesenchymal stem cells form Purkinje fibers in fetal sheep heart
    Judith A Airey
    Department of Pharmacology, MS 318, University of Nevada, Reno, Reno, NV 89523, USA
    Circulation 109:1401-7. 2004
    ..We have investigated the usefulness of a model of cardiac development in a large mammal, sheep, for studies of engraftment of human stem cells in the heart...
  7. ncbi request reprint Gestational age of recipient determines pattern and level of transgene expression following in utero retroviral gene transfer
    Christopher D Porada
    Department of Animal Biotechnology, University of Nevada at Reno, Reno, NV 89557, USA
    Mol Ther 11:284-93. 2005
    ..Our results suggest that with further vector optimization this approach may be useful for treating diseases that involve the lung and liver early in development...
  8. ncbi request reprint A human stromal-based serum-free culture system supports the ex vivo expansion/maintenance of bone marrow and cord blood hematopoietic stem/progenitor cells
    Cláudia Lobato da Silva
    Department of Animal Biotechnology, University of Nevada, Reno, 89557, USA
    Exp Hematol 33:828-35. 2005
    ..We investigated the role of human stromal layers (hu-ST) on the ex vivo expansion/maintenance of human hematopoietic stem/progenitor cells (HSC) from adult bone marrow (BM) and umbilical cord blood (CB)...
  9. doi request reprint Differences amid bone marrow and cord blood hematopoietic stem/progenitor cell division kinetics
    Cláudia Lobato da Silva
    Department of Animal Biotechnology, University of Nevada, Reno, Nevada 89557 0104, USA
    J Cell Physiol 220:102-11. 2009
    ....
  10. ncbi request reprint Transduction of long-term-engrafting human hematopoietic stem cells by retroviral vectors
    Christopher D Porada
    Department of Animal Biotechnology, University of Nevada, Reno, NV 89520, USA
    Hum Gene Ther 13:867-79. 2002
    ..The successful in vivo transduction of long-term engrafting human HSCs with the existing generation of murine retroviral vectors has significant implications for developing new approaches to pre- and postnatal gene therapy...
  11. pmc Persistent circulating human insulin in sheep transplanted in utero with human mesenchymal stem cells
    Adel Ersek
    Department of Animal Biotechnology, University of Nevada Reno, Reno, NV, USA
    Exp Hematol 38:311-20. 2010
    ..To determine if mesenchymal stem cells (MSC) derived from human fetal pancreatic tissue (pMSC) would engraft and differentiate in sheep pancreas following transplantation in utero...
  12. ncbi request reprint Differentiative potential of human metanephric mesenchymal cells
    Graca Almeida-Porada
    Department of Animal Biotechnology, University of Nevada Reno, Mail Stop 202, Reno, NV 89557 0104, USA
    Exp Hematol 30:1454-62. 2002
    ..To evaluate the ability of mesenchymal cells derived from nonhematopoietic organs to form blood and other tissues in vitro and in vivo...
  13. ncbi request reprint Human natural killer cell development in a xenogeneic culture system
    Isabel Barao
    University of Nevada School of Medicine, V A Medical Center, Reno, USA
    Br J Haematol 118:885-92. 2002
    ....
  14. ncbi request reprint Efficient generation of human hepatocytes by the intrahepatic delivery of clonal human mesenchymal stem cells in fetal sheep
    Jason Chamberlain
    Department of Animal Biotechnology, University of Nevada at Reno, Reno, NV 89557 0104, USA
    Hepatology 46:1935-45. 2007
    ..Furthermore, hepatocytes were generated from MSCs without the need to first migrate/lodge to the bone marrow and give rise to hematopoietic cells...
  15. ncbi request reprint A Stro-1(+) human universal stromal feeder layer to expand/maintain human bone marrow hematopoietic stem/progenitor cells in a serum-free culture system
    Raquel Gonçalves
    Department of Animal Biotechnology, University of Nevada, Reno, NV 89557, USA
    Exp Hematol 34:1353-9. 2006
    ..Furthermore, we compared the results obtained with MSC as a feeder layer to traditional allogeneic stromal layers grown in long-term bone marrow culture media (LT-ST)...
  16. pmc EphB2 isolates a human marrow stromal cell subpopulation with enhanced ability to contribute to the resident intestinal cellular pool
    Evan Colletti
    3Wake Forest School of Medicine, Institute for Regenerative Medicine, Medical Center Blvd, Winston Salem, NC 27157 1094, USA
    FASEB J 27:2111-21. 2013
    ..Mokhtari, S., Osborne, C., Schlauch, K., Zanjani, E. D., Porada, C. D., Almeida-Porada, G. EphB2 isolates a human marrow stromal cell subpopulation with enhanced ability to contribute to the resident intestinal cellular pool...
  17. doi request reprint Expression levels of the PiT-2 receptor explain, in part, the gestational age-dependent alterations in transduction efficiency after in utero retroviral-mediated gene transfer
    Ferhat Ozturk
    Department of Animal Biotechnology, University of Nevada, Reno, NV, USA
    J Gene Med 14:169-81. 2012
    ....
  18. pmc Phenotypic correction of hemophilia A in sheep by postnatal intraperitoneal transplantation of FVIII-expressing MSC
    Christopher D Porada
    Wake Forest Institute for Regenerative Medicine, Winston Salem, NC, USA
    Exp Hematol 39:1124-1135.e4. 2011
    ..In summary, this novel, nonablative MSC transplantation was straightforward, safe, and converted life-threatening, debilitating HA to a moderate phenotype in a large animal model...
  19. pmc Modulation of human mesenchymal stem cell immunogenicity through forced expression of human cytomegalovirus us proteins
    Melisa A Soland
    Department of Animal Biotechnology, University of Nevada, Reno, Nevada, United States of America
    PLoS ONE 7:e36163. 2012
    ..Human cytomegalovirus (HCMV) has developed several strategies to evade cytotoxic T lymphocyte (CTL) and Natural Killer (NK) cell recognition. Our goal is to exploit HCMV immunological evasion strategies to reduce MSC immunogenicity...
  20. ncbi request reprint Male germ-line cells are at risk following direct-injection retroviral-mediated gene transfer in utero
    Christopher D Porada
    Department of Animal Biotechnology, University of Nevada at Reno, 89557 0104, USA
    Mol Ther 12:754-62. 2005
    ..In conclusion, our analysis on over 3 x 10(9) sperm cells suggests that the direct-injection approach employed in these studies may result in the inadvertent transduction of very low numbers of male germ cells...
  21. ncbi request reprint The time course of engraftment of human mesenchymal stem cells in fetal heart demonstrates that Purkinje fiber aggregates derive from a single cell and not multi-cell homing
    Evan J Colletti
    Department of Pharmacology, University of Nevada, Reno, Reno NV 89557, USA
    Exp Hematol 34:926-33. 2006
    ..To study the early time course of engraftment of human mesenchymal stem cells in fetal sheep heart and determine the relative roles of proliferation and homing in formation of aggregates of human Purkinje fiber cells...
  22. ncbi request reprint A large animal noninjury model for study of human stem cell plasticity
    Graca Almeida-Porada
    Department of Animal Biotechnology, University of Nevada, Reno, NV 89557 0104, USA
    Blood Cells Mol Dis 32:77-81. 2004
    ....
  23. ncbi request reprint The sheep model of in utero gene therapy
    Christopher D Porada
    Department of Animal Biotechnology, University of Nevada, Reno, Nev 89557 0104, USA
    Fetal Diagn Ther 19:23-30. 2004
    ....
  24. ncbi request reprint Human haematopoietic stem cells that mediate long-term in vivo engraftment are not susceptible to infection by human cytomegalovirus
    Kirsten B Crapnell
    Department of Veterans Affairs Medical Center, University of Nevada Reno, Reno, NV, USA
    Br J Haematol 124:676-84. 2004
    ..These results provide evidence that the long-term engrafting HSC is not a primary target of HCMV and suggest that HCMV infection of human haematopoietic cells is exercised at the level of committed progenitors...
  25. pmc Mesenchymal stem cells as therapeutics and vehicles for gene and drug delivery
    Christopher D Porada
    Department of Animal Biotechnology, University of Nevada, Reno, 89557 0104, USA
    Adv Drug Deliv Rev 62:1156-66. 2010
    ....
  26. pmc Development and characterization of a novel CD34 monoclonal antibody that identifies sheep hematopoietic stem/progenitor cells
    Christopher D Porada
    Department of Animal Biotechnology, School of Veterinary Medicine, University of Nevada, Reno, NV 89557 0104, USA
    Exp Hematol 36:1739-49. 2008
    ..The current studies were undertaken to create a monoclonal antibody to sheep CD34 that would enable isolation and study of sheep HSC/progenitors...
  27. pmc Factors determining the risk of inadvertent retroviral transduction of male germ cells after in utero gene transfer in sheep
    Paul J Park
    Department of Animal Biotechnology, School of Veterinary Medicine, University of Nevada, Reno, NV 89557, USA
    Hum Gene Ther 20:201-15. 2009
    ....
  28. ncbi request reprint Reversible expression of CD34 by adult human bone marrow long-term engrafting hematopoietic stem cells
    Esmail D Zanjani
    Department of Animal Biotechnology, University of Nevada, Reno, Nev, USA
    Exp Hematol 31:406-12. 2003
    ....
  29. doi request reprint Mesenchymal stem cells contribute to endogenous FVIII:c production
    Chad Sanada
    Department of Animal Biotechnology, University of Nevada, Reno, NV, USA
    J Cell Physiol 228:1010-6. 2013
    ..These results demonstrate that MSC can contribute at low levels to the functional FVIII pool, and advance the understanding of the physiology of FVIII production and secretion...
  30. pmc Distinct contribution of human cord blood-derived endothelial colony forming cells to liver and gut in a fetal sheep model
    Joshua A Wood
    Department of Animal Biotechnology, University of Nevada, Reno, NV, USA
    Hepatology 56:1086-96. 2012
    ..Furthermore, ECFC expressing CD133 and CD117 lodged in areas where endogenous cells expressed those same phenotypes...
  31. doi request reprint Generation of tissue-specific cells from MSC does not require fusion or donor-to-host mitochondrial/membrane transfer
    Evan J Colletti
    Department of Animal Biotechnology, University of Nevada at Reno, Reno, NV 89557, USA
    Stem Cell Res 2:125-38. 2009
    ....
  32. pmc In vivo generation of beta-cell-like cells from CD34(+) cells differentiated from human embryonic stem cells
    A Daisy Goodrich
    Department of Animal Biotechnology, University of Nevada, Reno, Reno, NV 89557, USA
    Exp Hematol 38:516-525.e4. 2010
    ..Based on this observation, we explored the capacity of CD34(+) cells derived in culture from the differentiation of human embryonic stem cells (hESC), for their in vivo pancreatic endocrine capacity...
  33. ncbi request reprint Reduced stem cell factor links smooth myopathy and loss of interstitial cells of cajal in murine diabetic gastroparesis
    Viktor J Horváth
    Department of Physiology and Cell Biology, University of Nevada, Reno, Nevada, USA
    Gastroenterology 130:759-70. 2006
    ..Most murine gastric ICC depend on stem cell factor (SCF) signaling but can also be maintained with insulin or insulin-like growth factor-I (IGF-I). We investigated whether SCF could mediate the actions of insulin and IGF-I...
  34. pmc Generation of functional natural killer and dendritic cells in a human stromal-based serum-free culture system designed for cord blood expansion
    Ana M Frias
    Department of Animal Biotechnology, University of Nevada, Reno 89557 0104, USA
    Exp Hematol 36:61-8. 2008
    ..In this study, we investigated the ability of the CD7(+)CD34(-) population to differentiate into natural killer and dendritic cells (DCs)...
  35. ncbi request reprint Purification of interstitial cells of Cajal by fluorescence-activated cell sorting
    TAMAS ORDOG
    Department of Physiology and Cell Biology, University of Nevada, Reno School of Medicine, Anderson Bldg, Mail Stop 352, Reno, NV 89557, USA
    Am J Physiol Cell Physiol 286:C448-56. 2004
    ..Smooth muscle cells and myenteric neurons were also depleted. We conclude that immunofluorescence-based sorting can yield ICC in sufficiently high numbers and purity to permit detailed molecular analyses...
  36. pmc HCMV protein LUNA is required for viral reactivation from latently infected primary CD14⁺ cells
    Lisa R Keyes
    Department of Microbiology and Graduate Program in Cell and Molecular Biology, University of Nevada, Reno, Nevada, United States of America
    PLoS ONE 7:e52827. 2012
    ....
  37. pmc Early fetal gene delivery utilizes both central and peripheral mechanisms of tolerance induction
    Evan Colletti
    Department of Animal Biotechnology, University of Nevada, Reno, NV 89557 0104, USA
    Exp Hematol 36:816-22. 2008
    ..In the present studies, we conduct detailed analysis of the thymus of recipients of in utero gene transfer (IUGT) to delineate the mechanism of the observed immune tolerance and assess the impact of recipient age on this process...
  38. ncbi request reprint Docosahexaenoic acid induces dose dependent cell death in an early undifferentiated subtype of acute myeloid leukemia cell line
    Takashi Yamagami
    Department of Animal Biotechnology, University of Nevada, Reno, NV 89557 0104, USA
    Cancer Biol Ther 8:331-7. 2009
    ..Since we also show that DHA does not have a detrimental effect on normal hematopoiesis our results suggest that DHA could potentially serve as an well-tolerated adjuvant in the treatment of AML patients...

Research Grants10

  1. Functional Analysis of the Hematopoietic Niche during Ontogeny
    Graca Almeida Porada; Fiscal Year: 2009
    ..This would significantly improve the safety and success of clinical HSC transplantation and potentially allow its application to a wider range of diseases affecting human patients. ..
  2. In Utero Stem Cell Transplantation for Hemophilia A
    Graca Almeida Porada; Fiscal Year: 2006
    ..abstract_text> ..
  3. Evaluation of Stem Cell Plasticity In Vivo
    Graca Almeida Porada; Fiscal Year: 2004
    ..With these studies we hope to provide novel insights into the location of latent stem cell poois within the body and define the behavior and capabilities of these cells that are potentially of great clinical value. ..
  4. Functional Analysis of the Hematopoietic Niche during Ontogeny
    GRACA DUARTE ALMEIDA PORADA; Fiscal Year: 2010
    ..This would significantly improve the safety and success of clinical HSC transplantation and potentially allow its application to a wider range of diseases affecting human patients. ..