JAMES ALFANO

Summary

Affiliation: University of Nebraska
Country: USA

Publications

  1. ncbi request reprint Type III secretion system effector proteins: double agents in bacterial disease and plant defense
    James R Alfano
    The Plant Science Initiative and the Department of Plant Pathology, University of Nebraska, Lincoln, Nebraska, 68588 0660, USA
    Annu Rev Phytopathol 42:385-414. 2004
  2. pmc The hrpK operon of Pseudomonas syringae pv. tomato DC3000 encodes two proteins secreted by the type III (Hrp) protein secretion system: HopB1 and HrpK, a putative type III translocator
    Tanja Petnicki-Ocwieja
    Plant Science Initiative, The Beadle Center for Genetic Research, University of Nebraska, 1901 Vine St, Lincoln, NE 68588 0660, USA
    J Bacteriol 187:649-63. 2005
  3. pmc Roadmap for future research on plant pathogen effectors
    James R Alfano
    The Center for Plant Science Innovation and the Department of Plant Pathology, University of Nebraska, Lincoln, NE 68588 0660, USA
    Mol Plant Pathol 10:805-13. 2009
  4. ncbi request reprint Identification of Pseudomonas syringae type III effectors that can suppress programmed cell death in plants and yeast
    Yashitola Jamir
    Plant Science Initiative and Department of Plant Pathology, University of Nebraska, Lincoln, NE 68588 0660, USA
    Plant J 37:554-65. 2004
  5. pmc Plant immunity directly or indirectly restricts the injection of type III effectors by the Pseudomonas syringae type III secretion system
    Emerson Crabill
    Center for Plant Science Innovation, University of Nebraska, Lincoln, Nebraska 68588 0660, USA
    Plant Physiol 154:233-44. 2010
  6. ncbi request reprint Disabling surveillance: bacterial type III secretion system effectors that suppress innate immunity
    Avelina Espinosa
    Plant Science Initiative and The Department of Plant Pathology, University of Nebraska, Lincoln, NE 68588 0660, USA
    Cell Microbiol 6:1027-40. 2004
  7. pmc Pseudomonas syringae HrpJ is a type III secreted protein that is required for plant pathogenesis, injection of effectors, and secretion of the HrpZ1 Harpin
    Zheng Qing Fu
    Plant Science Initiative, The Beadle Center for Genetic Research, University of Nebraska, 1901 Vine St, Lincoln, NE 68588 0660, USA
    J Bacteriol 188:6060-9. 2006
  8. pmc Phytopathogen type III effector weaponry and their plant targets
    Anna Block
    Center for Plant Science Innovation and Department of Plant Pathology, University of Nebraska, Lincoln, NE 68588 0660, USA
    Curr Opin Plant Biol 11:396-403. 2008
  9. pmc The Pseudomonas syringae type III effector HopG1 targets mitochondria, alters plant development and suppresses plant innate immunity
    Anna Block
    The Center for Plant Science Innovation, University of Nebraska, Lincoln, Nebraska, USA
    Cell Microbiol 12:318-30. 2010
  10. pmc The Pseudomonas syringae HopPtoV protein is secreted in culture and translocated into plant cells via the type III protein secretion system in a manner dependent on the ShcV type III chaperone
    Misty D Wehling
    Plant Science Initiative and Department of Pathology, University of Nebraska, Lincoln, Nebraska 68588 0660, USA
    J Bacteriol 186:3621-30. 2004

Collaborators

  • David J Schneider
  • Xiaoyan Tang
  • G B Martin
  • Jian Min Zhou
  • Barbara N Kunkel
  • Gabriel Moreno-Hagelsieb
  • JEFFERY DANGL
  • JEAN GREENBERG
  • David S Guttman
  • John Stavrinides
  • Alan Collmer
  • Ming Guo
  • Zheng Qing Fu
  • Anna Block
  • Tanja Petnicki-Ocwieja
  • Yashitola Jamir
  • Fang Tian
  • Magdalen Lindeberg
  • Emerson Crabill
  • Avelina Espinosa
  • Karin van Dijk
  • Monica Vencato
  • Misty D Wehling
  • Libo Shan
  • C Robin Buell
  • Lisa M Schechter
  • Arun K Chatterjee
  • Guangyong Li
  • Vincent C Tam
  • Chia Fong Wei
  • Adriana O Ferreira
  • Christopher R Myers
  • John W Mansfield
  • Nai Chun Lin
  • Ping He
  • Samuel Cartinhour
  • Hye Sook Oh
  • Jong Hyun Ham
  • Yaya Cui
  • Scott T Chancey
  • Asita Chatterjee
  • Derrick E Fouts
  • Jennifer M van Rooyen
  • Anna Joe
  • Thomas E Clemente
  • Christian Elowsky
  • Yashitola Wamboldt
  • Dorothee Staiger
  • Ronald L Cerny
  • Brian H Kvitko
  • Hsiou Chen Huang
  • Byeong Ryool Jeong
  • Rena Shimizu
  • Thomas E Elthon
  • Genevieve A DeClerck
  • Vinita Joardar
  • Philip A Bronstein
  • Genevieve DeClerck
  • Samuel W Cartinhour
  • Warren F Lamboy
  • Jeffrey S Gordon
  • Jeffrey H Chang
  • Zhengxiang Ge
  • Lihuang Zhu
  • Pablo Rodriguez-Palenzuela
  • Xu Jia
  • Jianfu Chen
  • Kathy A Roberts
  • Zhongying Chen
  • Satya Chintamanani
  • Martin B Dickman
  • Clemencia M Rojas
  • Shaorong Chen
  • Hailian Yang
  • Angela M Baldo
  • Angela R Records
  • Michelle L Gwinn
  • Sondra G Lazarowitz
  • Misty D Janes
  • Mark D'Ascenzo
  • Robert B Abramovitch
  • Amos H Rehm

Detail Information

Publications27

  1. ncbi request reprint Type III secretion system effector proteins: double agents in bacterial disease and plant defense
    James R Alfano
    The Plant Science Initiative and the Department of Plant Pathology, University of Nebraska, Lincoln, Nebraska, 68588 0660, USA
    Annu Rev Phytopathol 42:385-414. 2004
    ....
  2. pmc The hrpK operon of Pseudomonas syringae pv. tomato DC3000 encodes two proteins secreted by the type III (Hrp) protein secretion system: HopB1 and HrpK, a putative type III translocator
    Tanja Petnicki-Ocwieja
    Plant Science Initiative, The Beadle Center for Genetic Research, University of Nebraska, 1901 Vine St, Lincoln, NE 68588 0660, USA
    J Bacteriol 187:649-63. 2005
    ..Taken together, HopB1 is a type III effector and HrpK plays an important role in the TTSS and is a putative type III translocator...
  3. pmc Roadmap for future research on plant pathogen effectors
    James R Alfano
    The Center for Plant Science Innovation and the Department of Plant Pathology, University of Nebraska, Lincoln, NE 68588 0660, USA
    Mol Plant Pathol 10:805-13. 2009
    ..Because effectors can be used as tools to elucidate components of innate immunity, advances in our understanding of effectors and their targets should lead to improvements in agriculture...
  4. ncbi request reprint Identification of Pseudomonas syringae type III effectors that can suppress programmed cell death in plants and yeast
    Yashitola Jamir
    Plant Science Initiative and Department of Plant Pathology, University of Nebraska, Lincoln, NE 68588 0660, USA
    Plant J 37:554-65. 2004
    ..The high proportion of effectors that suppress PCD suggests that suppressing plant immunity is one of the primary roles for DC3000 effectors and a central requirement for P. syringae pathogenesis...
  5. pmc Plant immunity directly or indirectly restricts the injection of type III effectors by the Pseudomonas syringae type III secretion system
    Emerson Crabill
    Center for Plant Science Innovation, University of Nebraska, Lincoln, Nebraska 68588 0660, USA
    Plant Physiol 154:233-44. 2010
    ..Our results show that PTI-induced HR inhibition is due to direct or indirect restriction of T3E injection and that T3Es can relieve this restriction by suppressing PTI...
  6. ncbi request reprint Disabling surveillance: bacterial type III secretion system effectors that suppress innate immunity
    Avelina Espinosa
    Plant Science Initiative and The Department of Plant Pathology, University of Nebraska, Lincoln, NE 68588 0660, USA
    Cell Microbiol 6:1027-40. 2004
    ..Understanding targets and activities of type III effectors will reveal much about bacterial pathogenicity and the innate immune system in plants and animals...
  7. pmc Pseudomonas syringae HrpJ is a type III secreted protein that is required for plant pathogenesis, injection of effectors, and secretion of the HrpZ1 Harpin
    Zheng Qing Fu
    Plant Science Initiative, The Beadle Center for Genetic Research, University of Nebraska, 1901 Vine St, Lincoln, NE 68588 0660, USA
    J Bacteriol 188:6060-9. 2006
    ....
  8. pmc Phytopathogen type III effector weaponry and their plant targets
    Anna Block
    Center for Plant Science Innovation and Department of Plant Pathology, University of Nebraska, Lincoln, NE 68588 0660, USA
    Curr Opin Plant Biol 11:396-403. 2008
    ..The wide array of strategies that bacterial pathogens employ to suppress innate immunity suggest that circumvention of innate immunity is crucial for bacterial pathogenicity of plants...
  9. pmc The Pseudomonas syringae type III effector HopG1 targets mitochondria, alters plant development and suppresses plant innate immunity
    Anna Block
    The Center for Plant Science Innovation, University of Nebraska, Lincoln, Nebraska, USA
    Cell Microbiol 12:318-30. 2010
    ..These findings suggest that HopG1's target is mitochondrial and that effector/target interaction promotes disease by disrupting mitochondrial functions...
  10. pmc The Pseudomonas syringae HopPtoV protein is secreted in culture and translocated into plant cells via the type III protein secretion system in a manner dependent on the ShcV type III chaperone
    Misty D Wehling
    Plant Science Initiative and Department of Pathology, University of Nebraska, Lincoln, Nebraska 68588 0660, USA
    J Bacteriol 186:3621-30. 2004
    ....
  11. ncbi request reprint A type III effector ADP-ribosylates RNA-binding proteins and quells plant immunity
    Zheng Qing Fu
    Plant Science Initiative and Department of Plant Pathology, University of Nebraska, Lincoln, Nebraska 68588 0660, USA
    Nature 447:284-8. 2007
    ..Our results suggest a pathogenic strategy where the ADP-ribosylation of RNA-binding proteins quells host immunity by affecting RNA metabolism and the plant defence transcriptome...
  12. pmc Genomewide identification of proteins secreted by the Hrp type III protein secretion system of Pseudomonas syringae pv. tomato DC3000
    Tanja Petnicki-Ocwieja
    Plant Science Initiative and Department of Plant Pathology, University of Nebraska, Lincoln, NE 68588 0660, USA
    Proc Natl Acad Sci U S A 99:7652-7. 2002
    ..One ADP-ribosyltransferase and the SrfC homolog were tested and shown to be secreted in a Hrp-dependent manner. These proteins, designated HopPtoS2 and HopPtoL, respectively, bring the DC3000 Hrp-secreted protein inventory to 22...
  13. pmc The majority of the type III effector inventory of Pseudomonas syringae pv. tomato DC3000 can suppress plant immunity
    Ming Guo
    The Center for Plant Science Innovation, University of Nebraska, Lincoln, Nebraska, USA
    Mol Plant Microbe Interact 22:1069-80. 2009
    ..Additionally, the construct pLN1965 will likely be a useful tool in determining whether other type III effectors or effectors from other types of pathogens can suppress either ETI, PTI, or both...
  14. ncbi request reprint The Pseudomonas syringae type III-secreted protein HopPtoD2 possesses protein tyrosine phosphatase activity and suppresses programmed cell death in plants
    Avelina Espinosa
    Plant Science Initiative, University of Nebraska Lincoln, NE 68588 0660, USA
    Mol Microbiol 49:377-87. 2003
    ..The identification of HopPtoD2 as a PTP and a PCD suppressor suggests that the inactivation of MAPK pathways is a virulence strategy utilized by bacterial plant pathogens...
  15. pmc Pseudomonas syringae type III chaperones ShcO1, ShcS1, and ShcS2 facilitate translocation of their cognate effectors and can substitute for each other in the secretion of HopO1-1
    Ming Guo
    Plant Science Initiative, University of Nebraska, 1901 Vine St, Lincoln, Nebraska 68588 0660, USA
    J Bacteriol 187:4257-69. 2005
    ..The existence of TTCs that can bind to dissimilar effectors and that can substitute for each other in effector secretion provides insights into the nature of how TTCs function...
  16. ncbi request reprint Activation of a COI1-dependent pathway in Arabidopsis by Pseudomonas syringae type III effectors and coronatine
    Ping He
    Department of Plant Pathology, Kansas State University, Manhattan, KS 66506, USA
    Plant J 37:589-602. 2004
    ..These results suggest that P. syringae type III effectors and coronatine act by augmenting a COI1-dependent pathway to promote parasitism...
  17. ncbi request reprint A Pseudomonas syringae pv. tomato DC3000 mutant lacking the type III effector HopQ1-1 is able to cause disease in the model plant Nicotiana benthamiana
    Chia Fong Wei
    Graduate Institute of Biotechnology, National Chung Hsing University, Taichung 40224, Taiwan
    Plant J 51:32-46. 2007
    ..syringae pathovars are limited by the complex interactions of effector repertoires with plant anti-effector surveillance systems, and they demonstrate that N. benthamiana can be a useful model host for DC3000...
  18. ncbi request reprint Genomic mining type III secretion system effectors in Pseudomonas syringae yields new picks for all TTSS prospectors
    Alan Collmer
    Dept of Plant Pathology, Cornell University, Ithaca, NY 14850 4203, USA
    Trends Microbiol 10:462-9. 2002
    ..syringae, potential effector targeting signals in all TTSS-dependent pathogens, and strategies for finding TTSS effectors in other bacteria that have sequenced genomes...
  19. ncbi request reprint The ShcA protein is a molecular chaperone that assists in the secretion of the HopPsyA effector from the type III (Hrp) protein secretion system of Pseudomonas syringae
    Karin van Dijk
    Department of Biological Sciences, University of Nevada, Las Vegas, NV 89154 4004, USA
    Mol Microbiol 44:1469-81. 2002
    ..Therefore, it is likely that chaperones are as prevalent in bacterial plant pathogen type III systems as they are in their animal pathogenic counterparts...
  20. ncbi request reprint The HopPtoF locus of Pseudomonas syringae pv. tomato DC3000 encodes a type III chaperone and a cognate effector
    Libo Shan
    Department of Plant Pathology, Kansas State University, Manhattan 66506 5502, USA
    Mol Plant Microbe Interact 17:447-55. 2004
    ..ShcF(Pto) interacts with and stabilizes the HopF(Pto) protein in the bacterial cell. Translation of HopF(Pto) starts at a rare initiation codon ATA that limits the synthesis of the HopF(Pto) protein to a low level in bacterial cells...
  21. ncbi request reprint Bioinformatics-enabled identification of the HrpL regulon and type III secretion system effector proteins of Pseudomonas syringae pv. phaseolicola 1448A
    Monica Vencato
    Department of Plant Pathology, Cornell University, Ithaca, NY 14853, USA
    Mol Plant Microbe Interact 19:1193-206. 2006
    ..These results establish the utility of the bioinformatic or candidate gene approach to identifying effectors and other genes relevant to pathogenesis in P. syringae genomes...
  22. ncbi request reprint Whole-genome expression profiling defines the HrpL regulon of Pseudomonas syringae pv. tomato DC3000, allows de novo reconstruction of the Hrp cis clement, and identifies novel coregulated genes
    Adriana O Ferreira
    Boyce Thompson Institute for Plant Research, Ithaca, NY 14853, USA
    Mol Plant Microbe Interact 19:1167-79. 2006
    ..syringae genomes, and it supports subsequent identification of effectors and other factors that likely are important to the host-specific virulence of P. syringae...
  23. ncbi request reprint Proposed guidelines for a unified nomenclature and phylogenetic analysis of type III Hop effector proteins in the plant pathogen Pseudomonas syringae
    Magdalen Lindeberg
    Department of Plant Pathology, Cornell University, Ithaca, NY 14853, USA
    Mol Plant Microbe Interact 18:275-82. 2005
    ..Phylogenetic analyses of previously characterized Hops are described, the results of which have been used to guide their integration into the proposed nomenclature...
  24. ncbi request reprint GacA, the response regulator of a two-component system, acts as a master regulator in Pseudomonas syringae pv. tomato DC3000 by controlling regulatory RNA, transcriptional activators, and alternate sigma factors
    Asita Chatterjee
    Department of Plant Microbiology and Pathology, University of Missouri, Columbia, MO, USA
    Mol Plant Microbe Interact 16:1106-17. 2003
    ..Our findings establish that GacA, located at the top in a regulatory cascade in DC3000, functions as a central regulator by controlling an assortment of transcriptional and posttranscriptional factors...
  25. pmc Pseudomonas syringae type III secretion system targeting signals and novel effectors studied with a Cya translocation reporter
    Lisa M Schechter
    Department of Plant Pathology, Cornell University, Ithaca, New York 14853 4203, USA
    J Bacteriol 186:543-55. 2004
    ..Our results indicate that Cya should be a useful reporter for exploring multiple aspects of the Hrp system in P. syringae...
  26. ncbi request reprint Analysis of Erwinia chrysanthemi EC16 pelE::uidA, pelL::uidA, and hrpN::uidA mutants reveals strain-specific atypical regulation of the Hrp type III secretion system
    Jong Hyun Ham
    Department of Plant Pathology, Cornell University, Ithaca, NY 14853 4203, USA
    Mol Plant Microbe Interact 17:184-94. 2004
    ..chrysanthemi in witloof chicory leaves. Overexpression of hrpN in E. chrysanthemi resulted in approximately 50% reduction of lesion size on chicory leaves without an effect on infection initiation...
  27. pmc Genomewide identification of Pseudomonas syringae pv. tomato DC3000 promoters controlled by the HrpL alternative sigma factor
    Derrick E Fouts
    Department of Plant Pathology, Cornell University, Ithaca, NY 14853 4203, USA
    Proc Natl Acad Sci U S A 99:2275-80. 2002
    ..Additional candidate effector genes, hopPtoA2, hopPtoB2, and an avrRps4 homolog, were preceded by Hrp promoter-like sequences, but these had HMM expectation values of relatively low significance and were not detectably activated by HrpL...