N Alexis

Summary

Affiliation: University of North Carolina
Country: USA

Publications

  1. ncbi request reprint Inhalation of low-dose endotoxin favors local T(H)2 response and primes airway phagocytes in vivo
    Neil E Alexis
    UNC School of Medicine, UNC Chapel Hill, Chapel Hill, NC 27599, USA
    J Allergy Clin Immunol 114:1325-31. 2004
  2. ncbi request reprint Exposures of healthy and asthmatic volunteers to concentrated ambient ultrafine particles in Los Angeles
    Henry Gong
    Keck School of Medicine, University of Southern California, Los Angeles, California, USA
    Inhal Toxicol 20:533-45. 2008
  3. pmc Individuals with increased inflammatory response to ozone demonstrate muted signaling of immune cell trafficking pathways
    Rebecca C Fry
    Department of Environmental Sciences and Engineering, Gillings School of Global Public Health, University of North Carolina, Chapel Hill, NC, USA
    Respir Res 13:89. 2012
  4. pmc In vivo particle uptake by airway macrophages in healthy volunteers
    Neil E Alexis
    Center for Environmental Medicine, Asthma, and Lung Biology, University of North Carolina at Chapel Hill, 104 Mason Farm Rd, Chapel Hill, NC 27599 7310, USA
    Am J Respir Cell Mol Biol 34:305-13. 2006
  5. doi request reprint Development of an inhaled endotoxin challenge protocol for characterizing evoked cell surface phenotype and genomic responses of airway cells in allergic individuals
    Neil E Alexis
    Center for Environmental Medicine, Asthma and Lung Biology, UNC School of Medicine, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA
    Ann Allergy Asthma Immunol 100:206-15. 2008
  6. pmc Fluticasone propionate protects against ozone-induced airway inflammation and modified immune cell activation markers in healthy volunteers
    Neil E Alexis
    Center for Environmental Medicine, Asthma and Lung Biology, University of North Carolina, Chapel Hill, North Carolina 27599 7310, USA
    Environ Health Perspect 116:799-805. 2008
  7. ncbi request reprint Biological material on inhaled coarse fraction particulate matter activates airway phagocytes in vivo in healthy volunteers
    Neil E Alexis
    Center for Environmental Medicine Asthma and Lung Biology, University of North Carolina at Chapel Hill, NC 27599 7310, USA
    J Allergy Clin Immunol 117:1396-403. 2006
  8. ncbi request reprint IL-4 induces IL-6 and signs of allergic-type inflammation in the nasal airways of nonallergic individuals
    Neil Alexis
    Department of Pediatrics, University of North Carolina at Chapel Hill, 27599, USA
    Clin Immunol 104:217-20. 2002
  9. ncbi request reprint Iron-binding and storage proteins in sputum
    Neil E Alexis
    Center for Environmental Medicine and Lung Biology, and Division of Pulmonary Medicine and Allergy, University of North Carolina, Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Inhal Toxicol 14:387-400. 2002
  10. ncbi request reprint Association between airway hyperreactivity and bronchial macrophage dysfunction in individuals with mild asthma
    N E Alexis
    Center for Environmental Medicine and Lung Biology, University of North Carolina, Chapel Hill 27599, North Carolina, USA
    Am J Physiol Lung Cell Mol Physiol 280:L369-75. 2001

Detail Information

Publications36

  1. ncbi request reprint Inhalation of low-dose endotoxin favors local T(H)2 response and primes airway phagocytes in vivo
    Neil E Alexis
    UNC School of Medicine, UNC Chapel Hill, Chapel Hill, NC 27599, USA
    J Allergy Clin Immunol 114:1325-31. 2004
    ..Conversely, we recently reported that very low doses of endotoxin, which are not associated with neutrophil influx, enhance response to allergen in the nasal and bronchial airway...
  2. ncbi request reprint Exposures of healthy and asthmatic volunteers to concentrated ambient ultrafine particles in Los Angeles
    Henry Gong
    Keck School of Medicine, University of Southern California, Los Angeles, California, USA
    Inhal Toxicol 20:533-45. 2008
    ....
  3. pmc Individuals with increased inflammatory response to ozone demonstrate muted signaling of immune cell trafficking pathways
    Rebecca C Fry
    Department of Environmental Sciences and Engineering, Gillings School of Global Public Health, University of North Carolina, Chapel Hill, NC, USA
    Respir Res 13:89. 2012
    ..The interplay between immuno-inflammatory function and genomic signaling in those with heightened inflammatory responsiveness to ozone is not well understood...
  4. pmc In vivo particle uptake by airway macrophages in healthy volunteers
    Neil E Alexis
    Center for Environmental Medicine, Asthma, and Lung Biology, University of North Carolina at Chapel Hill, 104 Mason Farm Rd, Chapel Hill, NC 27599 7310, USA
    Am J Respir Cell Mol Biol 34:305-13. 2006
    ..Further, macrophage recruitment and phagocytosis-modifying agents are factors in vivo that likely affect particle uptake and its time course...
  5. doi request reprint Development of an inhaled endotoxin challenge protocol for characterizing evoked cell surface phenotype and genomic responses of airway cells in allergic individuals
    Neil E Alexis
    Center for Environmental Medicine, Asthma and Lung Biology, UNC School of Medicine, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA
    Ann Allergy Asthma Immunol 100:206-15. 2008
    ..Inhaled endotoxin protocols have been used to evaluate airway cell surface phenotypes associated with antigen presentation and innate immunity in healthy volunteers, but not in allergic volunteers...
  6. pmc Fluticasone propionate protects against ozone-induced airway inflammation and modified immune cell activation markers in healthy volunteers
    Neil E Alexis
    Center for Environmental Medicine, Asthma and Lung Biology, University of North Carolina, Chapel Hill, North Carolina 27599 7310, USA
    Environ Health Perspect 116:799-805. 2008
    ..High-dose inhaled corticosteroids can reduce O(3)-induced airway inflammation, but their effect on innate immune activation is unknown...
  7. ncbi request reprint Biological material on inhaled coarse fraction particulate matter activates airway phagocytes in vivo in healthy volunteers
    Neil E Alexis
    Center for Environmental Medicine Asthma and Lung Biology, University of North Carolina at Chapel Hill, NC 27599 7310, USA
    J Allergy Clin Immunol 117:1396-403. 2006
    ..In vitro, endotoxin on coarse fraction particulate matter (PM2.5-10) accounts for the majority of the ability of PM2.5-10 to induce cytokine responses from alveolar macrophages...
  8. ncbi request reprint IL-4 induces IL-6 and signs of allergic-type inflammation in the nasal airways of nonallergic individuals
    Neil Alexis
    Department of Pediatrics, University of North Carolina at Chapel Hill, 27599, USA
    Clin Immunol 104:217-20. 2002
    ..These data demonstrate that IL-4 can modulate both allergic- and nonallergic-type inflammatory responses in the nasal airways of nonallergic individuals...
  9. ncbi request reprint Iron-binding and storage proteins in sputum
    Neil E Alexis
    Center for Environmental Medicine and Lung Biology, and Division of Pulmonary Medicine and Allergy, University of North Carolina, Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Inhal Toxicol 14:387-400. 2002
    ..The finding of decreased transferrin with increased ferritin suggests a local cellular response via iron-response elements (IRE) associated with the mRNAs for these two iron-binding proteins...
  10. ncbi request reprint Association between airway hyperreactivity and bronchial macrophage dysfunction in individuals with mild asthma
    N E Alexis
    Center for Environmental Medicine and Lung Biology, University of North Carolina, Chapel Hill 27599, North Carolina, USA
    Am J Physiol Lung Cell Mol Physiol 280:L369-75. 2001
    ..007), lung function (%predicted FEV(1), R = 0.7, P < 0.002) and percent eosinophils (R = -0.6, P < 0.01). In conclusion, BM from asthmatics are functionally modulated, possibly by Th2 cytokines involved in asthma pathology...
  11. pmc Low-level ozone exposure induces airways inflammation and modifies cell surface phenotypes in healthy humans
    Neil E Alexis
    Center for Environmental Medicine, Asthma and Lung Biology, University of North Carolina School of Medicine, 104 Mason Farm Road, Chapel Hill, NC 27599, USA
    Inhal Toxicol 22:593-600. 2010
    ..These findings need to be replicated in a similar experiment that includes a control air exposure...
  12. ncbi request reprint Cyclooxygenase metabolites play a different role in ozone-induced pulmonary function decline in asthmatics compared to normals
    N Alexis
    Center for Environmental Medicine and Lung Biology, University of North Carolina, Chapel Hill, 104 Mason Farm Rd, Chapel Hill, NC 27599 7310, USA
    Inhal Toxicol 12:1205-24. 2000
    ..Specifically, COX metabolites contribute to restrictive-type changes in normals and obstructive-type changes in small airways in asthmatics...
  13. ncbi request reprint Effect of inhaled endotoxin on airway and circulating inflammatory cell phagocytosis and CD11b expression in atopic asthmatic subjects
    Neil E Alexis
    Center for Environmental Medicine, Asthma and Lung Biology, University of North Carolina, Chapel Hill, 27599, USA
    J Allergy Clin Immunol 112:353-61. 2003
    ..Macrophage and neutrophil functions have been reported to be modified by endotoxin in vitro and in vivo, and response to endotoxin is mediated largely by airway phagocytes and related to allergic inflammation...
  14. pmc Attenuation of host defense function of lung phagocytes in young cystic fibrosis patients
    Neil E Alexis
    Center for Environmental Medicine, Asthma and Lung Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    J Cyst Fibros 5:17-25. 2006
    ....
  15. pmc The glutathione-S-transferase Mu 1 null genotype modulates ozone-induced airway inflammation in human subjects
    Neil E Alexis
    Center for Environmental Medicine, Asthma and Lung Biology, UNC School of Medicine, Chapel Hill, NC 27599 7310, USA
    J Allergy Clin Immunol 124:1222-1228.e5. 2009
    ..Ozone is known to cause an immediate decrease in lung function and increased airway inflammation. However, it is not known whether GSTM1 modulates these ozone responses in vivo in human subjects...
  16. ncbi request reprint Sputum phagocytes from healthy individuals are functional and activated: a flow cytometric comparison with cells in bronchoalveolar lavage and peripheral blood
    N Alexis
    Center for Environmental Medicine and Lung Biology, University of North Carolina, Chapel Hill, North Carolina, 27599 7310, USA
    Clin Immunol 97:21-32. 2000
    ..In conclusion sputum/bronchial phagocytes from healthy individuals express an inflammatory phenotype and are functionally more active than phagocytes from the alveolar airways and peripheral blood...
  17. ncbi request reprint CD14-dependent airway neutrophil response to inhaled LPS: role of atopy
    N Alexis
    Center for Environmental Medicine and Lung Biology, Department of Pediatrics, University of North Carolina-Chapel Hill, 27599-7310, USA
    J Allergy Clin Immunol 107:31-5. 2001
    ..CONCLUSION: Constitutive airway CD14 expression can predict the magnitude of the PMN response after inhaled LPS. Atopy appears to play a role in the level of CD14 expression and may contribute to LPS sensitivity in asthmatic subjects...
  18. pmc Atopic asthmatic subjects but not atopic subjects without asthma have enhanced inflammatory response to ozone
    Michelle L Hernandez
    Center for Environmental Medicine Asthma and Lung Biology, University of North Carolina School of Medicine, University of North Carolina at Chapel Hill, NC 27599 7310, USA
    J Allergy Clin Immunol 126:537-44.e1. 2010
    ..Ozone causes an immediate decrease in lung function and increased airway inflammation. The role of atopy and asthma in modulation of ozone-induced inflammation has not been determined...
  19. pmc Different expression ratio of S100A8/A9 and S100A12 in acute and chronic lung diseases
    Eva Lorenz
    Department of Rheumatology, TARC University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7280, USA
    Respir Med 102:567-73. 2008
    ..The results indicate that the regulation of human calgranulin expression and the ratio of S100A8/A9 to S100A12 may provide important insights in the mechanism of respiratory inflammation...
  20. doi request reprint Regulation of airway nucleotides in chronic lung diseases
    Charles R Esther
    Pediatric Pulmonology, University of North Carolina, Chapel Hill, NC, 27599, USA
    Subcell Biochem 55:75-93. 2011
    ..This chapter exposes the extensive rearrangement of the enzymatic network taking place in diseased airways, and identifies signaling pathways likely involved in the aberrant regulation of the airway purines...
  21. pmc Comparative airway inflammatory response of normal volunteers to ozone and lipopolysaccharide challenge
    Michelle L Hernandez
    Department of Pediatrics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7310, USA
    Inhal Toxicol 22:648-56. 2010
    ....
  22. pmc Neutrophilic inflammation is associated with altered airway hydration in stable asthmatics
    Ceila E Loughlin
    Department of Pediatrics, Division of Pediatric Pulmonology, The School of Medicine, University of North Carolina, 130 Mason Farm Road, 5th floor Bioinformatics Bldg, CB 7217, Chapel Hill, NC 27599 7217, USA
    Respir Med 104:29-33. 2010
    ..Using sputum percent solids, as an indicator of airway hydration, we sought relationships between airway hydration and other known markers of neutrophilic (TH1) and allergic (TH2) inflammation in stable asthma...
  23. pmc Influence of C-159T SNP of the CD14 gene promoter on lung function in smokers
    Haibo Zhou
    The Center for Environmental Medicine, Asthma and Lung Biology, The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
    Respir Med 103:1358-65. 2009
    ..The effect of CC genotype on severity of COPD is analogous with the effect of this genotype in risk for asthma. CD14 may be a factor in the pathophysiology of COPD, as it is in asthma and smoking-related cardiovascular diseases...
  24. pmc Mechanisms of LPS-induced CD40 expression in human peripheral blood monocytic cells
    Weidong Wu
    Center for Environmental Medicine, Asthma, and Lung Biology, University of North Carolina, 104 Mason Farm Road, Chapel Hill, NC 27599, USA
    Biochem Biophys Res Commun 379:573-7. 2009
    ..In summary, LPS-induced CD40 expression on human PBMCs through activation of NFkappaB and JNK, and partially through the induction of IFNgamma production...
  25. pmc Tracheobronchial air-liquid interface cell culture: a model for innate mucosal defense of the upper airways?
    Mehmet Kesimer
    Dept of Biochemistry and Biophysics, Univ of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Am J Physiol Lung Cell Mol Physiol 296:L92-L100. 2009
    ..This shows that the primary cell culture system is an important model for study of aspects of innate defense of the upper airways related specifically to mucus consisting solely of airway cell products...
  26. pmc Involvement of mitogen-activated protein kinases and NFkappaB in LPS-induced CD40 expression on human monocytic cells
    Weidong Wu
    Center for Environmental Medicine, Asthma, and Lung Biology, University of North Carolina, Chapel Hill, NC 27599, USA
    Toxicol Appl Pharmacol 228:135-43. 2008
    ..Moreover, blockage of MAPK and NFkappaB activation inhibited LPS-induced TLR4 expression. In summary, LPS-induced CD40 expression in monocytic cells involves MAPKs and NFkappaB...
  27. ncbi request reprint Acute LPS inhalation in healthy volunteers induces dendritic cell maturation in vivo
    Neil E Alexis
    Center for Environmental Biology, Asthma, and Lung Biology, University of North Carolina at Chapel Hill, NC 27599, USA
    J Allergy Clin Immunol 115:345-50. 2005
    ..We have been studying the innate immune response of airways cells of healthy human volunteers to inhaled LPS, a Toll-like receptor 4 (TLR4) ligand, and have shown that macrophage phagocytic capacity is blunted...
  28. pmc Combination treatment with high-dose vitamin C and alpha-tocopherol does not enhance respiratory-tract lining fluid vitamin C levels in asthmatics
    Michelle Hernandez
    The Center for Environmental Medicine, Asthma and Lung Biology, University of North Carolina School of Medicine, 104 Mason Farm Road, Chapel Hill, NC 27599 7310, USA
    Inhal Toxicol 21:173-81. 2009
    ..In conclusion, supplementation with alpha T and high-dose vitamin C does not augment vitamin C levels in the respiratory-tract lining fluid...
  29. pmc In vivo gamma-tocopherol supplementation decreases systemic oxidative stress and cytokine responses of human monocytes in normal and asthmatic subjects
    Jessica Wiser
    The Center for Environmental Medicine, Asthma and Lung Biology, University of North Carolina School of Medicine, Chapel Hill, NC 27599 7310, USA
    Free Radic Biol Med 45:40-9. 2008
    ..Further, in vitro treatment of human monocytes with gamma-CEHC and alpha-CEHC inhibits ROS generation and LPS-induced degradation of IkappaB and JNK activation...
  30. ncbi request reprint Inflammatory response of the airway to inhaled endotoxin correlates with body mass index in atopic patients with asthma but not in normal volunteers
    Neil E Alexis
    J Allergy Clin Immunol 117:1185-6. 2006
  31. ncbi request reprint Ozone enhances markers of innate immunity and antigen presentation on airway monocytes in healthy individuals
    John C Lay
    J Allergy Clin Immunol 120:719-22. 2007
  32. pmc Repetitive organic dust exposure in vitro impairs macrophage differentiation and function
    Jill A Poole
    Pulmonary, Critical Care, Sleep and Allergy Section, Department of Internal Medicine, University of Nebraska Medical Center, Omaha, Neb 68198 5300, USA
    J Allergy Clin Immunol 122:375-82, 382.e1-4. 2008
    ..Organic dust exposure in the agricultural industry results in significant airway disease and lung function decrease. Mononuclear phagocytes are key cells that mediate the inflammatory and innate immune response after dust exposure...
  33. ncbi request reprint Circulating CD11b expression correlates with the neutrophil response and airway mCD14 expression is enhanced following ozone exposure in humans
    Neil E Alexis
    National Health and Environmental Research Laboratory, US Environmental Protection Agency, Research Triangle Park, NC 27711, USA
    Clin Immunol 111:126-31. 2004
    ..Consequently, CD11b may be a useful biomarker for predicting susceptibility to airway neutrophilic inflammation caused by pollutants...
  34. ncbi request reprint Circulating neutrophil CD14 expression and the inverse association of ambient particulate matter on lung function in asthmatic children
    Erik R Svendsen
    Office of Research and Development, EPA, Research Triangle Park, North Carolina 29208, USA
    Ann Allergy Asthma Immunol 99:244-53. 2007
    ....
  35. ncbi request reprint Low-dose airborne endotoxin exposure enhances bronchial responsiveness to inhaled allergen in atopic asthmatics
    Brian Boehlecke
    J Allergy Clin Immunol 112:1241-3. 2003
  36. pmc Macrophage enrichment from induced sputum
    Liv I B Sikkeland
    Thorax 62:558-9. 2007