Research Topics
Species | N AlexisSummaryAffiliation: University of North Carolina Country: USA Publications
| Collaborators
|
Detail Information
Publications
Exposures of healthy and asthmatic volunteers to concentrated ambient ultrafine particles in Los AngelesHenry Gong
Keck School of Medicine, University of Southern California, Los Angeles, California, USA
Inhal Toxicol 20:533-45. 2008....
Individuals with increased inflammatory response to ozone demonstrate muted signaling of immune cell trafficking pathwaysRebecca C Fry
Department of Environmental Sciences and Engineering, Gillings School of Global Public Health, University of North Carolina, Chapel Hill, NC, USA
Respir Res 13:89. 2012..The interplay between immuno-inflammatory function and genomic signaling in those with heightened inflammatory responsiveness to ozone is not well understood...- In vivo particle uptake by airway macrophages in healthy volunteersNeil E Alexis
Center for Environmental Medicine, Asthma, and Lung Biology, University of North Carolina at Chapel Hill, 104 Mason Farm Rd, Chapel Hill, NC 27599 7310, USA
Am J Respir Cell Mol Biol 34:305-13. 2006..Further, macrophage recruitment and phagocytosis-modifying agents are factors in vivo that likely affect particle uptake and its time course... 
Development of an inhaled endotoxin challenge protocol for characterizing evoked cell surface phenotype and genomic responses of airway cells in allergic individualsNeil E Alexis
Center for Environmental Medicine, Asthma and Lung Biology, UNC School of Medicine, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA
Ann Allergy Asthma Immunol 100:206-15. 2008..Inhaled endotoxin protocols have been used to evaluate airway cell surface phenotypes associated with antigen presentation and innate immunity in healthy volunteers, but not in allergic volunteers...- Inhalation of low-dose endotoxin favors local T(H)2 response and primes airway phagocytes in vivoNeil E Alexis
UNC School of Medicine, UNC Chapel Hill, Chapel Hill, NC 27599, USA
J Allergy Clin Immunol 114:1325-31. 2004..Conversely, we recently reported that very low doses of endotoxin, which are not associated with neutrophil influx, enhance response to allergen in the nasal and bronchial airway... - Fluticasone propionate protects against ozone-induced airway inflammation and modified immune cell activation markers in healthy volunteersNeil E Alexis
Center for Environmental Medicine, Asthma and Lung Biology, University of North Carolina, Chapel Hill, North Carolina 27599 7310, USA
Environ Health Perspect 116:799-805. 2008..High-dose inhaled corticosteroids can reduce O(3)-induced airway inflammation, but their effect on innate immune activation is unknown... - Biological material on inhaled coarse fraction particulate matter activates airway phagocytes in vivo in healthy volunteersNeil E Alexis
Center for Environmental Medicine Asthma and Lung Biology, University of North Carolina at Chapel Hill, NC 27599 7310, USA
J Allergy Clin Immunol 117:1396-403. 2006..In vitro, endotoxin on coarse fraction particulate matter (PM2.5-10) accounts for the majority of the ability of PM2.5-10 to induce cytokine responses from alveolar macrophages... - IL-4 induces IL-6 and signs of allergic-type inflammation in the nasal airways of nonallergic individualsNeil Alexis
Department of Pediatrics, University of North Carolina at Chapel Hill, 27599, USA
Clin Immunol 104:217-20. 2002..These data demonstrate that IL-4 can modulate both allergic- and nonallergic-type inflammatory responses in the nasal airways of nonallergic individuals... - Iron-binding and storage proteins in sputumNeil E Alexis
Center for Environmental Medicine and Lung Biology, and Division of Pulmonary Medicine and Allergy, University of North Carolina, Chapel Hill, Chapel Hill, North Carolina 27599, USA
Inhal Toxicol 14:387-400. 2002..The finding of decreased transferrin with increased ferritin suggests a local cellular response via iron-response elements (IRE) associated with the mRNAs for these two iron-binding proteins... - Association between airway hyperreactivity and bronchial macrophage dysfunction in individuals with mild asthmaN E Alexis
Center for Environmental Medicine and Lung Biology, University of North Carolina, Chapel Hill 27599, North Carolina, USA
Am J Physiol Lung Cell Mol Physiol 280:L369-75. 2001..007), lung function (%predicted FEV(1), R = 0.7, P < 0.002) and percent eosinophils (R = -0.6, P < 0.01). In conclusion, BM from asthmatics are functionally modulated, possibly by Th2 cytokines involved in asthma pathology... - Low-level ozone exposure induces airways inflammation and modifies cell surface phenotypes in healthy humansNeil E Alexis
Center for Environmental Medicine, Asthma and Lung Biology, University of North Carolina School of Medicine, 104 Mason Farm Road, Chapel Hill, NC 27599, USA
Inhal Toxicol 22:593-600. 2010..These findings need to be replicated in a similar experiment that includes a control air exposure... - Cyclooxygenase metabolites play a different role in ozone-induced pulmonary function decline in asthmatics compared to normalsN Alexis
Center for Environmental Medicine and Lung Biology, University of North Carolina, Chapel Hill, 104 Mason Farm Rd, Chapel Hill, NC 27599 7310, USA
Inhal Toxicol 12:1205-24. 2000..Specifically, COX metabolites contribute to restrictive-type changes in normals and obstructive-type changes in small airways in asthmatics... - Effect of inhaled endotoxin on airway and circulating inflammatory cell phagocytosis and CD11b expression in atopic asthmatic subjectsNeil E Alexis
Center for Environmental Medicine, Asthma and Lung Biology, University of North Carolina, Chapel Hill, 27599, USA
J Allergy Clin Immunol 112:353-61. 2003.... - Attenuation of host defense function of lung phagocytes in young cystic fibrosis patientsNeil E Alexis
Center for Environmental Medicine, Asthma and Lung Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
J Cyst Fibros 5:17-25. 2006..These impairments can be reproduced by incubation of non-CF cells with NE, while SP-A can partially reverse them. Decreasing protease activity and increasing collectin activity may be beneficial in early CF... - The glutathione-S-transferase Mu 1 null genotype modulates ozone-induced airway inflammation in human subjectsNeil E Alexis
Center for Environmental Medicine, Asthma and Lung Biology, UNC School of Medicine, Chapel Hill, NC 27599 7310, USA
J Allergy Clin Immunol 124:1222-1228.e5. 2009..Ozone is known to cause an immediate decrease in lung function and increased airway inflammation. However, it is not known whether GSTM1 modulates these ozone responses in vivo in human subjects... - Sputum phagocytes from healthy individuals are functional and activated: a flow cytometric comparison with cells in bronchoalveolar lavage and peripheral bloodN Alexis
Center for Environmental Medicine and Lung Biology, University of North Carolina, Chapel Hill, North Carolina, 27599 7310, USA
Clin Immunol 97:21-32. 2000..In conclusion sputum/bronchial phagocytes from healthy individuals express an inflammatory phenotype and are functionally more active than phagocytes from the alveolar airways and peripheral blood... - CD14-dependent airway neutrophil response to inhaled LPS: role of atopyN Alexis
Center for Environmental Medicine and Lung Biology, Department of Pediatrics, University of North Carolina-Chapel Hill, 27599-7310, USA
J Allergy Clin Immunol 107:31-5. 2001..CONCLUSION: Constitutive airway CD14 expression can predict the magnitude of the PMN response after inhaled LPS. Atopy appears to play a role in the level of CD14 expression and may contribute to LPS sensitivity in asthmatic subjects... - Atopic asthmatic subjects but not atopic subjects without asthma have enhanced inflammatory response to ozoneMichelle L Hernandez
Center for Environmental Medicine Asthma and Lung Biology, University of North Carolina School of Medicine, University of North Carolina at Chapel Hill, NC 27599 7310, USA
J Allergy Clin Immunol 126:537-44.e1. 2010..Ozone causes an immediate decrease in lung function and increased airway inflammation. The role of atopy and asthma in modulation of ozone-induced inflammation has not been determined... - Different expression ratio of S100A8/A9 and S100A12 in acute and chronic lung diseasesEva Lorenz
Department of Rheumatology, TARC University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7280, USA
Respir Med 102:567-73. 2008..The results indicate that the regulation of human calgranulin expression and the ratio of S100A8/A9 to S100A12 may provide important insights in the mechanism of respiratory inflammation... - Regulation of airway nucleotides in chronic lung diseasesCharles R Esther
Pediatric Pulmonology, University of North Carolina, Chapel Hill, NC, 27599, USA
Subcell Biochem 55:75-93. 2011..This chapter exposes the extensive rearrangement of the enzymatic network taking place in diseased airways, and identifies signaling pathways likely involved in the aberrant regulation of the airway purines... - Comparative airway inflammatory response of normal volunteers to ozone and lipopolysaccharide challengeMichelle L Hernandez
Department of Pediatrics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7310, USA
Inhal Toxicol 22:648-56. 2010.... - Neutrophilic inflammation is associated with altered airway hydration in stable asthmaticsCeila E Loughlin
Department of Pediatrics, Division of Pediatric Pulmonology, The School of Medicine, University of North Carolina, 130 Mason Farm Road, 5th floor Bioinformatics Bldg, CB 7217, Chapel Hill, NC 27599 7217, USA
Respir Med 104:29-33. 2010..Using sputum percent solids, as an indicator of airway hydration, we sought relationships between airway hydration and other known markers of neutrophilic (TH1) and allergic (TH2) inflammation in stable asthma... - Influence of C-159T SNP of the CD14 gene promoter on lung function in smokersHaibo Zhou
The Center for Environmental Medicine, Asthma and Lung Biology, The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
Respir Med 103:1358-65. 2009..The effect of CC genotype on severity of COPD is analogous with the effect of this genotype in risk for asthma. CD14 may be a factor in the pathophysiology of COPD, as it is in asthma and smoking-related cardiovascular diseases... - Mechanisms of LPS-induced CD40 expression in human peripheral blood monocytic cellsWeidong Wu
Center for Environmental Medicine, Asthma, and Lung Biology, University of North Carolina, 104 Mason Farm Road, Chapel Hill, NC 27599, USA
Biochem Biophys Res Commun 379:573-7. 2009..In summary, LPS-induced CD40 expression on human PBMCs through activation of NFkappaB and JNK, and partially through the induction of IFNgamma production... - Tracheobronchial air-liquid interface cell culture: a model for innate mucosal defense of the upper airways?Mehmet Kesimer
Dept of Biochemistry and Biophysics, Univ of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
Am J Physiol Lung Cell Mol Physiol 296:L92-L100. 2009..This shows that the primary cell culture system is an important model for study of aspects of innate defense of the upper airways related specifically to mucus consisting solely of airway cell products... - Involvement of mitogen-activated protein kinases and NFkappaB in LPS-induced CD40 expression on human monocytic cellsWeidong Wu
Center for Environmental Medicine, Asthma, and Lung Biology, University of North Carolina, Chapel Hill, NC 27599, USA
Toxicol Appl Pharmacol 228:135-43. 2008..Moreover, blockage of MAPK and NFkappaB activation inhibited LPS-induced TLR4 expression. In summary, LPS-induced CD40 expression in monocytic cells involves MAPKs and NFkappaB... - Acute LPS inhalation in healthy volunteers induces dendritic cell maturation in vivoNeil E Alexis
Center for Environmental Biology, Asthma, and Lung Biology, University of North Carolina at Chapel Hill, NC 27599, USA
J Allergy Clin Immunol 115:345-50. 2005..We have been studying the innate immune response of airways cells of healthy human volunteers to inhaled LPS, a Toll-like receptor 4 (TLR4) ligand, and have shown that macrophage phagocytic capacity is blunted... - Combination treatment with high-dose vitamin C and alpha-tocopherol does not enhance respiratory-tract lining fluid vitamin C levels in asthmaticsMichelle Hernandez
The Center for Environmental Medicine, Asthma and Lung Biology, University of North Carolina School of Medicine, 104 Mason Farm Road, Chapel Hill, NC 27599 7310, USA
Inhal Toxicol 21:173-81. 2009..In conclusion, supplementation with alpha T and high-dose vitamin C does not augment vitamin C levels in the respiratory-tract lining fluid... - In vivo gamma-tocopherol supplementation decreases systemic oxidative stress and cytokine responses of human monocytes in normal and asthmatic subjectsJessica Wiser
The Center for Environmental Medicine, Asthma and Lung Biology, University of North Carolina School of Medicine, Chapel Hill, NC 27599 7310, USA
Free Radic Biol Med 45:40-9. 2008..Further, in vitro treatment of human monocytes with gamma-CEHC and alpha-CEHC inhibits ROS generation and LPS-induced degradation of IkappaB and JNK activation... - Inflammatory response of the airway to inhaled endotoxin correlates with body mass index in atopic patients with asthma but not in normal volunteersNeil E Alexis
J Allergy Clin Immunol 117:1185-6. 2006 - Ozone enhances markers of innate immunity and antigen presentation on airway monocytes in healthy individualsJohn C Lay
J Allergy Clin Immunol 120:719-22. 2007 - Repetitive organic dust exposure in vitro impairs macrophage differentiation and functionJill A Poole
Pulmonary, Critical Care, Sleep and Allergy Section, Department of Internal Medicine, University of Nebraska Medical Center, Omaha, Neb 68198 5300, USA
J Allergy Clin Immunol 122:375-82, 382.e1-4. 2008..Organic dust exposure in the agricultural industry results in significant airway disease and lung function decrease. Mononuclear phagocytes are key cells that mediate the inflammatory and innate immune response after dust exposure... - Circulating CD11b expression correlates with the neutrophil response and airway mCD14 expression is enhanced following ozone exposure in humansNeil E Alexis
National Health and Environmental Research Laboratory, US Environmental Protection Agency, Research Triangle Park, NC 27711, USA
Clin Immunol 111:126-31. 2004..Consequently, CD11b may be a useful biomarker for predicting susceptibility to airway neutrophilic inflammation caused by pollutants... - Circulating neutrophil CD14 expression and the inverse association of ambient particulate matter on lung function in asthmatic childrenErik R Svendsen
Office of Research and Development, EPA, Research Triangle Park, North Carolina 29208, USA
Ann Allergy Asthma Immunol 99:244-53. 2007.... - Low-dose airborne endotoxin exposure enhances bronchial responsiveness to inhaled allergen in atopic asthmaticsBrian Boehlecke
J Allergy Clin Immunol 112:1241-3. 2003 - Macrophage enrichment from induced sputumLiv I B Sikkeland
Thorax 62:558-9. 2007