Research Topics
Genomes and Genes | Oleg M AlekseevSummaryAffiliation: University of North Carolina Country: USA Publications
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Publications
Analysis of gene expression profiles in HeLa cells in response to overexpression or siRNA-mediated depletion of NASPOleg M Alekseev
Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, 27599 7090, USA
Reprod Biol Endocrinol 7:45. 2009..To understand the pathways and networks that may involve NASP function, we evaluated gene expression in HeLa cells in which NASP was either overexpressed or depleted by siRNA...
Depletion of the histone chaperone tNASP inhibits proliferation and induces apoptosis in prostate cancer PC-3 cellsOleg M Alekseev
Department of Cell and Developmental Biology, University of North Carolina, Chapel Hill, NC 27599, USA
Reprod Biol Endocrinol 9:50. 2011..Only cancer, germ, transformed, and embryonic cells have a high level of expression of the tNASP splice variant. We examined the consequences of tNASP depletion for prostate cancer PC-3 cells...
Nuclear autoantigenic sperm protein (NASP), a linker histone chaperone that is required for cell proliferationRichard T Richardson
Department of Cell and Developmental Biology, University of North Carolina, Chapel Hill, 27599, USA
J Biol Chem 281:21526-34. 2006..We conclude from this study that NASP and therefore the linker histones are key players in the assembly of chromatin after DNA replication...
Mass spectrometry identification of NASP binding partners in HeLa cellsOleg M Alekseev
Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill, 27599 7090, USA
Proteins 61:1-5. 2005..This new method can be used for expedited identification of binding partners of different proteins in enriched fractions and as a complementary or alternative strategy to the yeast two-hybrid system and immunoprecipitation methods...
Association of NASP with HSP90 in mouse spermatogenic cells: stimulation of ATPase activity and transport of linker histones into nucleiOleg M Alekseev
Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7090
J Biol Chem 280:2904-11. 2005..NASP-H1 is subsequently released from the complex and translocates to the nucleus where the H1 is released for binding to the DNA...
Characterization of the NASP promoter in 3T3 fibroblasts and mouse spermatogenic cellsRichard T Richardson
Department of Cell and Developmental Biology and Laboratories for Reproductive Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
Gene 371:52-8. 2006..Supershift assays further confirmed the presence of Sp1 bound to their respective sites suggesting that Sp1 and Ets are the primary activators of the NASP promoter...
Linker histones stimulate HSPA2 ATPase activity through NASP binding and inhibit CDC2/Cyclin B1 complex formation during meiosis in the mouseOleg M Alekseev
Department of Cell and Developmental Biology and the Laboratories for Reproductive Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA
Biol Reprod 81:739-48. 2009....
Overexpression of the Linker histone-binding protein tNASP affects progression through the cell cycleOleg M Alekseev
Department of Cell and Developmental Biology, University of North Carolina, Chapel Hill 27599 7090, USA
J Biol Chem 278:8846-52. 2003..These data suggest that NASP-H1 complexes exist in the nucleus and that tNASP can influence cell cycle progression through the G(1)/S border through mediation of DNA-H1 histone binding...
