Hui Wang Ai
Affiliation: University of California
- A highly efficient oxidative condensation reaction for selective protein conjugationAo Ji
Department of Chemistry, University of California Riverside, 501 Big Springs Road, Riverside, CA 92521, USA
Chem Commun (Camb) 50:7469-72. 2014..We demonstrated that the reaction could be utilized to label a T4 lysozyme protein containing a chemically installed aryl diamine group with a fluorescent aldehyde dye molecule at 37 °C. ..
- Genetically encoded fluorescent redox probesWei Ren
Department of Chemistry, University of California, 501 Big Springs Road, Riverside, CA 92521, USA
Sensors (Basel) 13:15422-33. 2013..Our recent work on reaction-based encoded probes that are responsive to particular redox signaling molecules is also reviewed. Future challenges and directions are also commented. ..
- Biochemical analysis with the expanded genetic lexiconHui Wang Ai
Department of Chemistry, University of California, Riverside, CA 92521, USA
Anal Bioanal Chem 403:2089-102. 2012..This review summarizes the methodology of genetic code expansion and its recent progress, and discusses the applications of commonly used analytical methods...
- Genetically encoded fluorescent probe for the selective detection of peroxynitriteZhi Jie Chen
Department of Chemistry, University of California, Riverside, California 92521, United States
J Am Chem Soc 135:14940-3. 2013..The new probe has been genetically introduced into mammalian cells to image peroxynitrite at physiologically relevant concentrations. ..
- Probing protein-protein interactions with a genetically encoded photo-crosslinking amino acidHui Wang Ai
Department of Chemistry, The Scripps Research Institute, La Jolla, CA 92037, USA
Chembiochem 12:1854-7. 2011
- Reaction-based genetically encoded fluorescent hydrogen sulfide sensorsSi Chen
Department of Chemistry, University of California, 501 Big Springs Road, Riverside, California 92521, USA
J Am Chem Soc 134:9589-92. 2012..Exploration of a circularly permuted FP led to an improved sensor with faster responses, and the feasibility of using such a genetically encoded probe to monitor H(2)S in living mammalian cells has also been demonstrated...