Jinwoo Ahn

Summary

Affiliation: University of Pittsburgh
Country: USA

Publications

  1. pmc HIV-1 Vpr loads uracil DNA glycosylase-2 onto DCAF1, a substrate recognition subunit of a cullin 4A-ring E3 ubiquitin ligase for proteasome-dependent degradation
    Jinwoo Ahn
    Department of Structural Biology, University of Pittsburgh School of Medicine and Cancer Institute, Pittsburgh, Pennsylvania 15260, USA
    J Biol Chem 285:37333-41. 2010
  2. pmc The RNA binding protein HuR does not interact directly with HIV-1 reverse transcriptase and does not affect reverse transcription in vitro
    Jinwoo Ahn
    Department of Structural Biology, Division of Infectious Diseases, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA
    Retrovirology 7:40. 2010
  3. pmc HIV-2 and SIVmac accessory virulence factor Vpx down-regulates SAMHD1 enzyme catalysis prior to proteasome-dependent degradation
    Maria Delucia
    Department of Structural Biology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15260, USA
    J Biol Chem 288:19116-26. 2013
  4. pmc The Cullin-RING E3 ubiquitin ligase CRL4-DCAF1 complex dimerizes via a short helical region in DCAF1
    Jinwoo Ahn
    University of Pittsburgh Center for HIV Protein Interactions, University of Pittsburgh School of Medicine, Pittsburgh, PA 15260, USA
    Biochemistry 50:1359-67. 2011
  5. pmc NMR structure of human restriction factor APOBEC3A reveals substrate binding and enzyme specificity
    In Ja L Byeon
    Department of Structural Biology, University of Pittsburgh School of Medicine, 3501 Fifth Avenue, Pittsburgh, PA 15261, USA
    Nat Commun 4:1890. 2013
  6. pmc Motions on the millisecond time scale and multiple conformations of HIV-1 capsid protein: implications for structural polymorphism of CA assemblies
    In Ja L Byeon
    Pittsburgh Center for HIV Protein Interactions, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA
    J Am Chem Soc 134:6455-66. 2012
  7. pmc Rhesus TRIM5α disrupts the HIV-1 capsid at the inter-hexamer interfaces
    Gongpu Zhao
    Department of Structural Biology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, United States of America
    PLoS Pathog 7:e1002009. 2011
  8. pmc Protease cleavage leads to formation of mature trimer interface in HIV-1 capsid
    Xin Meng
    Department of Structural Biology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, United States of America
    PLoS Pathog 8:e1002886. 2012
  9. pmc Mature HIV-1 capsid structure by cryo-electron microscopy and all-atom molecular dynamics
    Gongpu Zhao
    Department of Structural Biology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15260, USA
    Nature 497:643-6. 2013
  10. pmc Structure of the HIV-1 full-length capsid protein in a conformationally trapped unassembled state induced by small-molecule binding
    Shoucheng Du
    Department of Structural Biology, University of Pittsburgh Medical School, Pittsburgh, PA 15260, USA
    J Mol Biol 406:371-86. 2011

Collaborators

Detail Information

Publications21

  1. pmc HIV-1 Vpr loads uracil DNA glycosylase-2 onto DCAF1, a substrate recognition subunit of a cullin 4A-ring E3 ubiquitin ligase for proteasome-dependent degradation
    Jinwoo Ahn
    Department of Structural Biology, University of Pittsburgh School of Medicine and Cancer Institute, Pittsburgh, Pennsylvania 15260, USA
    J Biol Chem 285:37333-41. 2010
    ..Taken together, our results show that the CRL4(DCAF1) E3 ubiquitin ligase can be subverted by Vpr to target UNG2 for degradation...
  2. pmc The RNA binding protein HuR does not interact directly with HIV-1 reverse transcriptase and does not affect reverse transcription in vitro
    Jinwoo Ahn
    Department of Structural Biology, Division of Infectious Diseases, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA
    Retrovirology 7:40. 2010
    ....
  3. pmc HIV-2 and SIVmac accessory virulence factor Vpx down-regulates SAMHD1 enzyme catalysis prior to proteasome-dependent degradation
    Maria Delucia
    Department of Structural Biology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15260, USA
    J Biol Chem 288:19116-26. 2013
    ....
  4. pmc The Cullin-RING E3 ubiquitin ligase CRL4-DCAF1 complex dimerizes via a short helical region in DCAF1
    Jinwoo Ahn
    University of Pittsburgh Center for HIV Protein Interactions, University of Pittsburgh School of Medicine, Pittsburgh, PA 15260, USA
    Biochemistry 50:1359-67. 2011
    ....
  5. pmc NMR structure of human restriction factor APOBEC3A reveals substrate binding and enzyme specificity
    In Ja L Byeon
    Department of Structural Biology, University of Pittsburgh School of Medicine, 3501 Fifth Avenue, Pittsburgh, PA 15261, USA
    Nat Commun 4:1890. 2013
    ..Our results afford novel mechanistic insights into APOBEC3A-mediated deamination and provide the structural basis for further molecular studies...
  6. pmc Motions on the millisecond time scale and multiple conformations of HIV-1 capsid protein: implications for structural polymorphism of CA assemblies
    In Ja L Byeon
    Pittsburgh Center for HIV Protein Interactions, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA
    J Am Chem Soc 134:6455-66. 2012
    ..These findings suggest that the presence of structural plasticity at the CTD dimer interface permits pleiotropic HIV-1 capsid assembly, resulting in varied capsid morphologies...
  7. pmc Rhesus TRIM5α disrupts the HIV-1 capsid at the inter-hexamer interfaces
    Gongpu Zhao
    Department of Structural Biology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, United States of America
    PLoS Pathog 7:e1002009. 2011
    ....
  8. pmc Protease cleavage leads to formation of mature trimer interface in HIV-1 capsid
    Xin Meng
    Department of Structural Biology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, United States of America
    PLoS Pathog 8:e1002886. 2012
    ..Collectively, our results indicate that the proteolytic cleavage of Gag leads to a structural reorganization of the polypeptide and creates the three-fold interhexamer interface, important for the formation of infectious HIV-1 particles...
  9. pmc Mature HIV-1 capsid structure by cryo-electron microscopy and all-atom molecular dynamics
    Gongpu Zhao
    Department of Structural Biology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15260, USA
    Nature 497:643-6. 2013
    ..The complete atomic HIV-1 capsid model provides a platform for further studies of capsid function and for targeted pharmacological intervention...
  10. pmc Structure of the HIV-1 full-length capsid protein in a conformationally trapped unassembled state induced by small-molecule binding
    Shoucheng Du
    Department of Structural Biology, University of Pittsburgh Medical School, Pittsburgh, PA 15260, USA
    J Mol Biol 406:371-86. 2011
    ..Based on our findings, we propose an unprecedented means of preventing CA assembly, by "conformationally trapping" CA in assembly-incompetent conformational states induced by H-site binding...
  11. pmc 1H, 15N and 13C assignments of the dimeric C-terminal domain of HIV-1 capsid protein
    Jinwon Jung
    Department of Structural Biology and Pittsburgh Center for HIV Protein Interactions, University of Pittsburgh School of Medicine, Pittsburgh, PA 15260, USA
    Biomol NMR Assign 4:21-3. 2010
    ..Here, we present nearly complete (1)H, (15)N and (13)C assignments for the 20 kDa homodimeric CA-CTD, a prerequisite for structural characterization of the CA-CTD dimer...
  12. pmc Structural basis of the allosteric inhibitor interaction on the HIV-1 reverse transcriptase RNase H domain
    Martin T Christen
    Department of Structural Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15260, USA
    Chem Biol Drug Des 80:706-16. 2012
    ..2011) Chem Biol Drug Des 77, 39-47], but with slight differences that reflect the characteristics of the amino acid sequences in p15-EC compared to the WT RNH...
  13. pmc Structural convergence between Cryo-EM and NMR reveals intersubunit interactions critical for HIV-1 capsid function
    In Ja L Byeon
    Department of Structural Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15260, USA
    Cell 139:780-90. 2009
    ..In the tubular assembly, CA intermolecular interfaces vary slightly, accommodating the asymmetry present in tubes. This provides the necessary plasticity to allow for controlled virus capsid dis/assembly...
  14. pmc HIV/simian immunodeficiency virus (SIV) accessory virulence factor Vpx loads the host cell restriction factor SAMHD1 onto the E3 ubiquitin ligase complex CRL4DCAF1
    Jinwoo Ahn
    Department of Structural Biology and Pittsburgh Center for HIV Protein Interactions, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15260, USA
    J Biol Chem 287:12550-8. 2012
    ..Our findings show that the N terminus of Vpx, bound to DCAF1, recruits SAMHD1 via its C terminus to CRL4, in a species-specific manner for proteasomal degradation...
  15. pmc Solid-state NMR studies of HIV-1 capsid protein assemblies
    Yun Han
    Pittsburgh Center for HIV Protein Interactions, University of Pittsburgh School of Medicine, 1051 Biomedical Science Tower 3, 3501 Fifth Avenue, Pittsburgh, Pennsylvania 15261, USA
    J Am Chem Soc 132:1976-87. 2010
    ..The present results demonstrate that the assemblies of CA protein are amenable to detailed structural analysis by solid-state NMR spectroscopy...
  16. pmc Structure, dynamics, and Hck interaction of full-length HIV-1 Nef
    Jinwon Jung
    Department of Structural Biology and Pittsburgh Center for HIV Protein Interactions, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15260, USA
    Proteins 79:1609-22. 2011
    ..The RDC-based model combined with (15)N backbone dynamics data suggest that Hck32L adopts an open conformation without binding of the polyproline motif in the linker to the SH3 domain...
  17. doi request reprint Structural determinants of human APOBEC3A enzymatic and nucleic acid binding properties
    Mithun Mitra
    Section on Viral Gene Regulation, Program on Genomics of Differentiation, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892 2780, USA, Department of Structural Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA, Pittsburgh Center for HIV Protein Interactions, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA and HIV Drug Resistance Program, Frederick National Laboratory for Cancer Research, National Cancer Institute, Frederick, MD 21702, USA
    Nucleic Acids Res 42:1095-110. 2014
    ..Taken together, our studies provide new insights into the molecular properties of A3A and its role in multiple cellular and antiviral functions. ..
  18. pmc Insight into the structural basis of pro- and antiapoptotic p53 modulation by ASPP proteins
    Jinwoo Ahn
    Department of Structural Biology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15260, USA
    J Biol Chem 284:13812-22. 2009
    ..Our detailed structural analyses of the ASPP-p53 interactions provide insight into the structural basis of the differential behavior of pro- and antiapoptotic ASPP family members...
  19. doi request reprint Characterization of sarcoplasmic reticulum Ca2+ ATPase nucleotide binding domain mutants using NMR spectroscopy
    Wazo Myint
    Department of Structural Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15260, USA
    Biochem Biophys Res Commun 405:19-23. 2011
    ..8 mM). These results indicate that the E412G mutant retains its catalytic activity but most likely reduces its stability. Our findings provide molecular insight into previous clinical, physiological, and biochemical observations...
  20. pmc Interaction of HIV-1 reverse transcriptase ribonuclease H with an acylhydrazone inhibitor
    Qingguo Gong
    Department of Structural Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15260, USA
    Chem Biol Drug Des 77:39-47. 2011
    ..Our results provide a structural characterization of the ribonuclease H inhibitor interaction and are likely to be useful for further improvements of the inhibitors...
  21. pmc Dissection of the sequence-specific DNA binding and exonuclease activities reveals a superactive yet apoptotically impaired mutant p53 protein
    Jinwoo Ahn
    Department of Structural Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA
    Cell Cycle 8:1603-15. 2009
    ..They also extend previous findings showing that cell cycle arrest and apoptosis are separable functions of p53. Finally, these experiments confirm that DNA binding and xonuclease activities are distinct features of the p53 core domain...