Affiliation: University of Pittsburgh
Koharudin L, Wu Y, Delucia M, Mehrens J, Gronenborn A, Ahn J. Structural basis of allosteric activation of sterile Î± motif and histidine-aspartate domain-containing protein 1 (SAMHD1) by nucleoside triphosphates. J Biol Chem. 2014;289:32617-27 pubmed publisher
..Altogether, the data presented here advance our understanding of SAMHD1 function during cellular homeostasis. ..
Wu Y, Koharudin L, Mehrens J, Delucia M, Byeon C, Byeon I, et al
. Structural Basis of Clade-specific Engagement of SAMHD1 (Sterile Î± Motif and Histidine/Aspartate-containing Protein 1) Restriction Factors by Lentiviral Viral Protein X (Vpx) Virulence Factors. J Biol Chem. 2015;290:17935-45 pubmed publisher
Zhou X, Delucia M, Ahn J. SLX4-SLX1 Protein-independent Down-regulation of MUS81-EME1 Protein by HIV-1 Viral Protein R (Vpr). J Biol Chem. 2016;291:16936-16947 pubmed publisher
..We also show that neither the interaction of MUS81-EME1 with Vpr nor their down-regulation is dependent on SLX4-SLX1. Together, these data provide new insight on a conserved function of Vpr in a host endonuclease down-regulation. ..
Zhou X, Delucia M, Hao C, Hrecka K, Monnie C, Skowronski J, et al
. HIV-1 Vpr protein directly loads helicase-like transcription factor (HLTF) onto the CRL4-DCAF1 E3 ubiquitin ligase. J Biol Chem. 2017;292:21117-21127 pubmed publisher
..Thus, our findings reveal that Vpr utilizes common as well as distinctive interfaces to recruit multiple postreplication DNA repair proteins to the CRL4-DCAF1 E3 ligase for ubiquitin-dependent proteasomal degradation. ..
Ahn J, Vu T, Novince Z, Guerrero Santoro J, Rapic Otrin V, Gronenborn A. HIV-1 Vpr loads uracil DNA glycosylase-2 onto DCAF1, a substrate recognition subunit of a cullin 4A-ring E3 ubiquitin ligase for proteasome-dependent degradation. J Biol Chem. 2010;285:37333-41 pubmed publisher
..Taken together, our results show that the CRL4(DCAF1) E3 ubiquitin ligase can be subverted by Vpr to target UNG2 for degradation. ..