Research Topics
| PAUL G AHLQUISTSummaryAffiliation: University of Wisconsin Country: USA Publications
Research Grants
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Detail Information
Publications
Parallels among positive-strand RNA viruses, reverse-transcribing viruses and double-stranded RNA virusesPaul Ahlquist
Institute for Molecular Virology and Howard Hughes Medical Institute, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
Nat Rev Microbiol 4:371-82. 2006..Therefore, at least four of seven principal virus classes share several underlying features in genome replication and might have emerged from common ancestors. This has implications for virus function, evolution and control...- Virus evolution: fitting lifestyles to a TPaul Ahlquist
Howard Hughes Medical Institute and Institute for Molecular Virology, University of Wisconsin, Madison, WI, USA
Curr Biol 15:R465-7. 2005..These similarities intersect with emerging parallels in RNA replication to create intriguing evolutionary possibilities... - Viral and host determinants of RNA virus vector replication and expressionPaul Ahlquist
Institute for Molecular Virology, University of Wisconsin Madison, 1525 Linden Drive, Madison, WI 53706, USA
Vaccine 23:1784-7. 2005.... 
Bromovirus RNA replication compartment formation requires concerted action of 1a's self-interacting RNA capping and helicase domainsArturo Diaz
Institute for Molecular Virologya and Howard Hughes Medical Institute, Madison, Madison, Wisconsin 53706, USA
J Virol 86:821-34. 2012..These and other findings shed light on the roles and interactions of 1a domains in replication compartment formation and support prior results suggesting that 1a induces replication vesicles by forming a capsid-like interior shell...- Mutual interference between genomic RNA replication and subgenomic mRNA transcription in brome mosaic virusValery Z Grdzelishvili
Institute for Molecular Virology, University of Wisconsin-Madison, 1525 Linden Dr, Madison, WI 53706-1596, USA
J Virol 79:1438-51. 2005.... - Brome mosaic virus 1a nucleoside triphosphatase/helicase domain plays crucial roles in recruiting RNA replication templatesXiaofeng Wang
Institute for Molecular Virology, University of Wisconsin-Madison, 1525 Linden Dr, Madison, WI 53706-1576, USA
J Virol 79:13747-58. 2005..The possibility that, similar to some double-stranded RNA viruses, the 1a NTPase/helicase-like domain may be involved in importing viral RNAs into a preformed replication compartment is discussed... - Alternate, virus-induced membrane rearrangements support positive-strand RNA virus genome replicationMichael Schwartz
Institute for Molecular Virology and Howard Hughes Medical Institute, University of Wisconsin, Madison, WI 53706, USA
Proc Natl Acad Sci U S A 101:11263-8. 2004.... - Detecting differential gene expression with a semiparametric hierarchical mixture methodMichael A Newton
Department of Statistics, University of Wisconsin Madison, 1210 West Dayton St, Madison, WI 53706 1685, USA
Biostatistics 5:155-76. 2004..Compared to several competing methodologies, the proposed methodology exhibits good operating characteristics in a simulation study, on the analysis of spike-in data, and in a cross-validation calculation... - Systematic, genome-wide identification of host genes affecting replication of a positive-strand RNA virusDavid B Kushner
Institute for Molecular Virology and Howard Hughes Medical Institute, University of Wisconsin, Madison, WI 53706, USA
Proc Natl Acad Sci U S A 100:15764-9. 2003..The results further illuminate virus and cell pathways. Further refinement of virus screening likely will reveal contributions from additional host genes... - Membrane synthesis, specific lipid requirements, and localized lipid composition changes associated with a positive-strand RNA virus RNA replication proteinWai Ming Lee
Howard Hughes Medical Institute and Institute for Molecular Virology, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
J Virol 77:12819-28. 2003..This localized UFA depletion helps to explain why BMV RNA replication is more sensitive than cell growth to reduced UFA levels. The results imply that 1a preferentially interacts with one or more types of membrane lipids... - Long-distance base pairing in flock house virus RNA1 regulates subgenomic RNA3 synthesis and RNA2 replicationBrett D Lindenbach
Howard Hughes Medical Institute, University of Wisconsin-Madison, Madison, Wisconsin 53706-1596, USA
J Virol 76:3905-19. 2002..In addition, replication of an RNA2 derivative was dependent on RNA1 templates capable of forming the long-distance interaction that controls RNA3 production... 
Organelle-like membrane compartmentalization of positive-strand RNA virus replication factoriesJohan A den Boon
Institute for Molecular Virology, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
Annu Rev Microbiol 64:241-56. 2010..Our understanding of these complexes is growing, providing substantial new insights into the organization, coordination, and potential control of crucial processes in virus replication...- RNA-dependent RNA polymerases, viruses, and RNA silencingPaul Ahlquist
Howard Hughes Medical Institute and Institute for Molecular Virology, 1525 Linden Drive, University of Wisconsin Madison, Madison, WI 53706, USA
Science 296:1270-3. 2002..Better understanding of viral and host RdRps will improve our ability to control viruses and to use RNA silencing and viruses as tools for research, biotechnology, and medicine... - Membrane-shaping host reticulon proteins play crucial roles in viral RNA replication compartment formation and functionArturo Diaz
Institute for Molecular Virology, University of Wisconsin, Madison, WI 53706, USA
Proc Natl Acad Sci U S A 107:16291-6. 2010..The results suggest possible RHP roles in the bodies and necks of replication vesicles... - An alternate pathway for recruiting template RNA to the brome mosaic virus RNA replication complexJianbo Chen
Institute for Molecular Virology, University of Wisconsin, 1525 Linden Drive, Madison, WI 53706-1596, USA
J Virol 77:2568-77. 2003..Interaction with nascent 2a also may be involved in 1a recruitment of 2a polymerase to membranes... - Brome mosaic virus RNA replication: revealing the role of the host in RNA virus replicationAmine O Noueiry
Institute for Molecular Virology, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
Annu Rev Phytopathol 41:77-98. 2003..Characterizing the functions of these host factors is revealing basic aspects of virus RNA replication and helping to define the normal functions of these factors in the host... - Yeast Lsm1p-7p/Pat1p deadenylation-dependent mRNA-decapping factors are required for brome mosaic virus genomic RNA translationAmine O Noueiry
Institute for Molecular Virology. Howard Hughes Medical Institute, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA
Mol Cell Biol 23:4094-106. 2003.... - Host factors in positive-strand RNA virus genome replicationPaul Ahlquist
Institute for Molecular Virology, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA
J Virol 77:8181-6. 2003 - Involvement of host cellular multivesicular body functions in hepatitis B virus buddingTokiko Watanabe
Institute for Molecular Virology, McArdle Laboratory for Cancer Research, University of Wisconsin, 1525 Linden Drive, Madison, WI 53706, USA
Proc Natl Acad Sci U S A 104:10205-10. 2007..Moreover, HBV enveloped virions, enveloped subviral particles, and unenveloped nucleocapsids are all released by distinct pathways with separate host factor requirements... - Nodavirus RNA replication protein a induces membrane association of genomic RNAPriscilla M Van Wynsberghe
Institute for Molecular Virology, University of Wisconsin Madison, 1525 Linden Drive, Madison, WI 53706 1596, USA
J Virol 81:4633-44. 2007..These and other results show that prior to negative-strand RNA synthesis, multiple domains of mitochondrially targeted protein A cooperate to selectively recruit FHV genomic RNA to membranes where RNA replication complexes form... - An amphipathic alpha-helix controls multiple roles of brome mosaic virus protein 1a in RNA replication complex assembly and functionLing Liu
Institute for Molecular Virology, University of Wisconsin Madison, Madison, Wisconsin, USA
PLoS Pathog 5:e1000351. 2009.... - Production of infectious human papillomavirus independently of viral replication and epithelial cell differentiationDohun Pyeon
McArdle Laboratory for Cancer Research, Institute for Molecular Virology, University of Wisconsin, Madison, WI 53706, USA
Proc Natl Acad Sci U S A 102:9311-6. 2005.... - A positive-strand RNA virus replication complex parallels form and function of retrovirus capsidsMichael Schwartz
Institute for Molecular Virology and Howard Hughes Medical Institute, University of Wisconsin, Madison, Madison, WI 53706, USA
Mol Cell 9:505-14. 2002.... - Engineered retargeting of viral RNA replication complexes to an alternative intracellular membraneDavid J Miller
Department of Medicine, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA
J Virol 77:12193-202. 2003..We conclude that specific intracellular membranes are not required for FHV RNA replication complex formation and function... - 5' cis elements direct nodavirus RNA1 recruitment to mitochondrial sites of replication complex formationPriscilla M Van Wynsberghe
Institute for Molecular Virology, University of Wisconsin Madison, Madison, Wisconsin 53706 1596, USA
J Virol 83:2976-88. 2009..These results establish RNA recruitment to the sites of replication complex formation as an essential, distinct, and selective early step in nodavirus replication... - The host cell sulfonation pathway contributes to retroviral infection at a step coincident with provirus establishmentJames W Bruce
Institute for Molecular Virology, University of Wisconsin, Madison, WI, USA
PLoS Pathog 4:e1000207. 2008.... 
Three-dimensional analysis of a viral RNA replication complex reveals a virus-induced mini-organelleBenjamin G Kopek
Institute for Molecular Virology, University of Wisconsin Madison, Madison, Wisconsin, United States of America
PLoS Biol 5:e220. 2007..The resulting insights appear relevant to many other positive-strand RNA viruses and support recently proposed structural and likely evolutionary parallels with retrovirus and double-stranded RNA virus virions...- Cytoplasmic viral replication complexesJohan A den Boon
Institute for Molecular Virology, University of Wisconsin Madison, Madison, WI 53706, USA
Cell Host Microbe 8:77-85. 2010.... - Fundamental differences in cell cycle deregulation in human papillomavirus-positive and human papillomavirus-negative head/neck and cervical cancersDohun Pyeon
McArdle Laboratory for Cancer Research, University of Wisconsin Madison, Madison, WI 53706, USA
Cancer Res 67:4605-19. 2007.... - Establishment of human papillomavirus infection requires cell cycle progressionDohun Pyeon
McArdle Laboratory for Cancer Research, University of Wisconsin Madison, Madison, WI, USA
PLoS Pathog 5:e1000318. 2009..By defining a major condition for cell susceptibility to HPV infection, these results also have potentially important implications for HPV control... - Statistical use of argonaute expression and RISC assembly in microRNA target identificationStephen A Stanhope
Department of Statistics, University of Wisconsin Madison, Madison, Wisconsin, USA
PLoS Comput Biol 5:e1000516. 2009.... - Flock house virus RNA polymerase is a transmembrane protein with amino-terminal sequences sufficient for mitochondrial localization and membrane insertionDavid J Miller
University of Wisconsin Madison, Madison, Wisconsin 53706, USA
J Virol 76:9856-67. 2002..We conclude that protein A targets and anchors FHV RNA replication complexes to outer mitochondrial membranes, in part through an N-proximal mitochondrial localization signal and transmembrane domain... - Isolation of cell lines that show novel, murine leukemia virus-specific blocks to early steps of retroviral replicationJames W Bruce
Institute for Molecular Virology, University of Wisconsin, Madison, 53706-1596, USA
J Virol 79:12969-78. 2005..These cell lines are important tools for the biochemical and genetic analysis of early stages in retrovirus infection... - Filling a GAP(DH) in asymmetric viral RNA synthesisXiaofeng Wang
Institute for Molecular Virology, University of Wisconsin, Madison, WI 53706, USA
Cell Host Microbe 3:124-5. 2008..GAPDH binds TBSV (-)RNA and promotes the normal excess of (+)RNA over (-)RNA products, possibly by selectively retaining (-)RNA templates for copying... - In vivo self-interaction of nodavirus RNA replicase protein a revealed by fluorescence resonance energy transferBilly T Dye
Howard Hughes Medical Institute, University of Wisconsin-Madison, Madison, WI 53706, USA
J Virol 79:8909-19. 2005..Thus, the results imply that FHV protein A functions as a multimer rather than as a monomer at one or more steps in RNA replication... - High-throughput isolation of Saccharomyces cerevisiae RNABilly T Dye
University of Wisconsin-Madison, Madison, WI, USA
Biotechniques 38:868, 870. 2005 - Genome-wide expression profiling reveals EBV-associated inhibition of MHC class I expression in nasopharyngeal carcinomaSrikumar Sengupta
Institute for Molecular Virology, McArdle Laboratory for Cancer Research, University of Wisconsin-Madison, Madison, WI 53706, USA
Cancer Res 66:7999-8006. 2006.... - Cellular transcription factor ZASC1 regulates murine leukemia virus transcriptionJames W Bruce
Institute for Molecular Virology, University of Wisconsin, Madison, Wisconsin 53706, USA
J Virol 84:7473-83. 2010..These studies identify the cellular transcription factor ZASC1 as an activator of MLV gene expression and provide tools that should be useful in studying the links between ZASC1 and human diseases... - Intersection of the multivesicular body pathway and lipid homeostasis in RNA replication by a positive-strand RNA virusXiaofeng Wang
Institute for Molecular Virology, University of Wisconsin, Madison, WI 53706, USA
J Virol 85:5494-503. 2011..Among other points, these findings show the potential for blocking viral RNA replication by modulating lipid synthesis at multiple levels... - Detecting protein-protein interaction in live yeast by flow cytometryBilly T Dye
Howard Hughes Medical Institute, University of Wisconsin, 1525 Linden Drive, Madison, WI 53706, USA
Cytometry A 63:77-86. 2005..Probability binning is also employed to provide a statistical confirmation of our observations... - Drosophila RNAi screen identifies host genes important for influenza virus replicationLinhui Hao
Institute for Molecular Virology, USA
Nature 454:890-3. 2008.... - MicroRNA 29c is down-regulated in nasopharyngeal carcinomas, up-regulating mRNAs encoding extracellular matrix proteinsSrikumar Sengupta
Institute for Molecular Virology, Howard Hughes Medical Institute, McArdle Laboratory for Cancer Research, Departments of Statistics and Biostatistics and Medical Informatics, University of Wisconsin, Madison, WI 53076, USA
Proc Natl Acad Sci U S A 105:5874-8. 2008..Thus, we identify eight miRNAs differentially expressed in NPC and demonstrate the involvement of one in regulating genes involved in metastasis...
Research Grants
- GENE FUNCTIONS AND MOLECULAR GENETICS OF RNA VIRUSESPaul Ahlquist; Fiscal Year: 2007..The results will advance understanding and control of widespread viral diseases, and understanding of normal cell functions relevant to other disease processes. ..
- Novel Approaches to Detect Virus-Cancer AssociationsPaul Ahlquist; Fiscal Year: 2005..The methods developed also will be applicable to probe for virus involvement in other chronic or acute disorders. ..
- GENE FUNCTIONS AND MOLECULAR GENETICS OF RNA VIRUSESPaul Ahlquist; Fiscal Year: 1993....
- GENE FUNCTIONS AND MOLECULAR GENETICS OF RNA VIRUSESPaul Ahlquist; Fiscal Year: 2009..The results will advance understanding and control of widespread viral diseases, and understanding of normal cell functions relevant to other disease processes. ..