PAUL G AHLQUIST

Summary

Affiliation: University of Wisconsin
Country: USA

Publications

  1. pmc An amphipathic alpha-helix controls multiple roles of brome mosaic virus protein 1a in RNA replication complex assembly and function
    Ling Liu
    Institute for Molecular Virology, University of Wisconsin Madison, Madison, Wisconsin, USA
    PLoS Pathog 5:e1000351. 2009
  2. pmc The host cell sulfonation pathway contributes to retroviral infection at a step coincident with provirus establishment
    James W Bruce
    Institute for Molecular Virology, University of Wisconsin, Madison, WI, USA
    PLoS Pathog 4:e1000207. 2008
  3. pmc ZASC1 stimulates HIV-1 transcription elongation by recruiting P-TEFb and TAT to the LTR promoter
    James W Bruce
    Morgridge Institute for Research, Madison, Wisconsin, United States of America Institute for Molecular Virology, University of Wisconsin, Madison, Wisconsin, United States of America McArdle Laboratory for Cancer Research, University of Wisconsin, Madison, Wisconsin, United States of America
    PLoS Pathog 9:e1003712. 2013
  4. pmc Systematic identification of novel, essential host genes affecting bromovirus RNA replication
    Brandi L Gancarz
    Institute for Molecular Virology, University of Wisconsin Madison, Madison, Wisconsin, United States of America
    PLoS ONE 6:e23988. 2011
  5. pmc Genome-wide analysis of host factors in nodavirus RNA replication
    Linhui Hao
    Institute for Molecular Virology, University of Wisconsin Madison, Madison, Wisconsin, United States of America Howard Hughes Medical Institute, University of Wisconsin Madison, Madison, Wisconsin, United States of America
    PLoS ONE 9:e95799. 2014
  6. pmc Bromovirus RNA replication compartment formation requires concerted action of 1a's self-interacting RNA capping and helicase domains
    Arturo Diaz
    Institute for Molecular Virologya and Howard Hughes Medical Institute, Madison, Madison, Wisconsin 53706, USA
    J Virol 86:821-34. 2012
  7. ncbi request reprint Parallels among positive-strand RNA viruses, reverse-transcribing viruses and double-stranded RNA viruses
    Paul Ahlquist
    Institute for Molecular Virology and Howard Hughes Medical Institute, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    Nat Rev Microbiol 4:371-82. 2006
  8. ncbi request reprint Virus evolution: fitting lifestyles to a T
    Paul Ahlquist
    Howard Hughes Medical Institute and Institute for Molecular Virology, University of Wisconsin, Madison, WI, USA
    Curr Biol 15:R465-7. 2005
  9. ncbi request reprint Viral and host determinants of RNA virus vector replication and expression
    Paul Ahlquist
    Institute for Molecular Virology, University of Wisconsin Madison, 1525 Linden Drive, Madison, WI 53706, USA
    Vaccine 23:1784-7. 2005
  10. pmc Mutual interference between genomic RNA replication and subgenomic mRNA transcription in brome mosaic virus
    Valery Z Grdzelishvili
    Institute for Molecular Virology, University of Wisconsin Madison, 1525 Linden Dr, Madison, WI 53706 1596, USA
    J Virol 79:1438-51. 2005

Collaborators

  • Michael Newton
  • M N Gould
  • Wai Ming Lee
  • D J Miller
  • Amine O Noueiry
  • M W Schwartz
  • Stephen A Stanhope
  • Meng Chen
  • Guy Perkins
  • Yoshihiro Kawaoka
  • Xiaofeng Wang
  • James W Bruce
  • Johan A den Boon
  • Arturo Diaz
  • Linhui Hao
  • Dohun Pyeon
  • Billy T Dye
  • Srikumar Sengupta
  • John A T Young
  • Priscilla M Van Wynsberghe
  • Tokiko Watanabe
  • Paul F Lambert
  • Qiuling He
  • Valery Z Grdzelishvili
  • Brandi L Gancarz
  • Ling Liu
  • Brett D Lindenbach
  • Allan Hildesheim
  • Chien Jen Chen
  • Benjamin G Kopek
  • Bill Sugden
  • Yu Juen Cheng
  • I How Chen
  • Jianbo Chen
  • David B Kushner
  • David Kushner
  • Brett Lindenbach
  • Billy Dye
  • Megan Bracken
  • Elizabeth Mathieu
  • Rachel Reddington
  • Andreas Gallei
  • Michael Hierl
  • William M Westler
  • Simon M Lank
  • H Adam Steinberg
  • Shane M Pearce
  • Akira Sakurai
  • Ericka Sorensen
  • Chairul A Nidom
  • Karl T Kelsey
  • Lubomir P Turek
  • Seungtaek Kim
  • Thomas H Haugen
  • Ericka M Sorensen
  • Akira Naito
  • Craig D Woodworth
  • Elaine M Smith
  • Joseph P Connor
  • Hau Ren Chen
  • Meghan Schott
  • Carmen J Marsit
  • Mark H Ellisman
  • David B Dahl
  • William H Westra
  • Hernan Garcia-Ruiz
  • Kenneth A Bradley
  • Kathleen Schell
  • Scott M Paul
  • Jean Yves Sgro

Detail Information

Publications45

  1. pmc An amphipathic alpha-helix controls multiple roles of brome mosaic virus protein 1a in RNA replication complex assembly and function
    Ling Liu
    Institute for Molecular Virology, University of Wisconsin Madison, Madison, Wisconsin, USA
    PLoS Pathog 5:e1000351. 2009
    ....
  2. pmc The host cell sulfonation pathway contributes to retroviral infection at a step coincident with provirus establishment
    James W Bruce
    Institute for Molecular Virology, University of Wisconsin, Madison, WI, USA
    PLoS Pathog 4:e1000207. 2008
    ....
  3. pmc ZASC1 stimulates HIV-1 transcription elongation by recruiting P-TEFb and TAT to the LTR promoter
    James W Bruce
    Morgridge Institute for Research, Madison, Wisconsin, United States of America Institute for Molecular Virology, University of Wisconsin, Madison, Wisconsin, United States of America McArdle Laboratory for Cancer Research, University of Wisconsin, Madison, Wisconsin, United States of America
    PLoS Pathog 9:e1003712. 2013
    ..Thus, we have identified ZASC1 as novel regulator of HIV-1 gene expression that functions through the DNA-dependent, RNA-independent recruitment of TAT/P-TEFb to the HIV-1 promoter. ..
  4. pmc Systematic identification of novel, essential host genes affecting bromovirus RNA replication
    Brandi L Gancarz
    Institute for Molecular Virology, University of Wisconsin Madison, Madison, Wisconsin, United States of America
    PLoS ONE 6:e23988. 2011
    ..Investigating the diverse functions of these newly identified essential host genes should advance our understanding of BMV-host interactions and normal cellular pathways, and suggest new modes of virus control...
  5. pmc Genome-wide analysis of host factors in nodavirus RNA replication
    Linhui Hao
    Institute for Molecular Virology, University of Wisconsin Madison, Madison, Wisconsin, United States of America Howard Hughes Medical Institute, University of Wisconsin Madison, Madison, Wisconsin, United States of America
    PLoS ONE 9:e95799. 2014
    ....
  6. pmc Bromovirus RNA replication compartment formation requires concerted action of 1a's self-interacting RNA capping and helicase domains
    Arturo Diaz
    Institute for Molecular Virologya and Howard Hughes Medical Institute, Madison, Madison, Wisconsin 53706, USA
    J Virol 86:821-34. 2012
    ..These and other findings shed light on the roles and interactions of 1a domains in replication compartment formation and support prior results suggesting that 1a induces replication vesicles by forming a capsid-like interior shell...
  7. ncbi request reprint Parallels among positive-strand RNA viruses, reverse-transcribing viruses and double-stranded RNA viruses
    Paul Ahlquist
    Institute for Molecular Virology and Howard Hughes Medical Institute, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    Nat Rev Microbiol 4:371-82. 2006
    ..Therefore, at least four of seven principal virus classes share several underlying features in genome replication and might have emerged from common ancestors. This has implications for virus function, evolution and control...
  8. ncbi request reprint Virus evolution: fitting lifestyles to a T
    Paul Ahlquist
    Howard Hughes Medical Institute and Institute for Molecular Virology, University of Wisconsin, Madison, WI, USA
    Curr Biol 15:R465-7. 2005
    ..These similarities intersect with emerging parallels in RNA replication to create intriguing evolutionary possibilities...
  9. ncbi request reprint Viral and host determinants of RNA virus vector replication and expression
    Paul Ahlquist
    Institute for Molecular Virology, University of Wisconsin Madison, 1525 Linden Drive, Madison, WI 53706, USA
    Vaccine 23:1784-7. 2005
    ....
  10. pmc Mutual interference between genomic RNA replication and subgenomic mRNA transcription in brome mosaic virus
    Valery Z Grdzelishvili
    Institute for Molecular Virology, University of Wisconsin Madison, 1525 Linden Dr, Madison, WI 53706 1596, USA
    J Virol 79:1438-51. 2005
    ....
  11. pmc Membrane synthesis, specific lipid requirements, and localized lipid composition changes associated with a positive-strand RNA virus RNA replication protein
    Wai Ming Lee
    Howard Hughes Medical Institute and Institute for Molecular Virology, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    J Virol 77:12819-28. 2003
    ..This localized UFA depletion helps to explain why BMV RNA replication is more sensitive than cell growth to reduced UFA levels. The results imply that 1a preferentially interacts with one or more types of membrane lipids...
  12. pmc Yeast Lsm1p-7p/Pat1p deadenylation-dependent mRNA-decapping factors are required for brome mosaic virus genomic RNA translation
    Amine O Noueiry
    Institute for Molecular Virology Howard Hughes Medical Institute, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    Mol Cell Biol 23:4094-106. 2003
    ....
  13. ncbi request reprint Organelle-like membrane compartmentalization of positive-strand RNA virus replication factories
    Johan A den Boon
    Institute for Molecular Virology, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    Annu Rev Microbiol 64:241-56. 2010
    ..Our understanding of these complexes is growing, providing substantial new insights into the organization, coordination, and potential control of crucial processes in virus replication...
  14. pmc An alternate pathway for recruiting template RNA to the brome mosaic virus RNA replication complex
    Jianbo Chen
    Institute for Molecular Virology, University of Wisconsin, 1525 Linden Drive, Madison, WI 53706 1596, USA
    J Virol 77:2568-77. 2003
    ..Interaction with nascent 2a also may be involved in 1a recruitment of 2a polymerase to membranes...
  15. pmc Membrane-shaping host reticulon proteins play crucial roles in viral RNA replication compartment formation and function
    Arturo Diaz
    Institute for Molecular Virology, University of Wisconsin, Madison, WI 53706, USA
    Proc Natl Acad Sci U S A 107:16291-6. 2010
    ..The results suggest possible RHP roles in the bodies and necks of replication vesicles...
  16. ncbi request reprint Brome mosaic virus RNA replication: revealing the role of the host in RNA virus replication
    Amine O Noueiry
    Institute for Molecular Virology, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    Annu Rev Phytopathol 41:77-98. 2003
    ..Characterizing the functions of these host factors is revealing basic aspects of virus RNA replication and helping to define the normal functions of these factors in the host...
  17. ncbi request reprint RNA-dependent RNA polymerases, viruses, and RNA silencing
    Paul Ahlquist
    Howard Hughes Medical Institute and Institute for Molecular Virology, 1525 Linden Drive, University of Wisconsin Madison, Madison, WI 53706, USA
    Science 296:1270-3. 2002
    ..Better understanding of viral and host RdRps will improve our ability to control viruses and to use RNA silencing and viruses as tools for research, biotechnology, and medicine...
  18. pmc Long-distance base pairing in flock house virus RNA1 regulates subgenomic RNA3 synthesis and RNA2 replication
    Brett D Lindenbach
    Howard Hughes Medical Institute, University of Wisconsin Madison, Madison, Wisconsin 53706 1596, USA
    J Virol 76:3905-19. 2002
    ..In addition, replication of an RNA2 derivative was dependent on RNA1 templates capable of forming the long-distance interaction that controls RNA3 production...
  19. pmc Brome mosaic virus 1a nucleoside triphosphatase/helicase domain plays crucial roles in recruiting RNA replication templates
    Xiaofeng Wang
    Institute for Molecular Virology, University of Wisconsin Madison, 1525 Linden Dr, Madison, WI 53706 1576, USA
    J Virol 79:13747-58. 2005
    ..The possibility that, similar to some double-stranded RNA viruses, the 1a NTPase/helicase-like domain may be involved in importing viral RNAs into a preformed replication compartment is discussed...
  20. pmc Alternate, virus-induced membrane rearrangements support positive-strand RNA virus genome replication
    Michael Schwartz
    Institute for Molecular Virology and Howard Hughes Medical Institute, University of Wisconsin, Madison, WI 53706, USA
    Proc Natl Acad Sci U S A 101:11263-8. 2004
    ....
  21. pmc Systematic, genome-wide identification of host genes affecting replication of a positive-strand RNA virus
    David B Kushner
    Institute for Molecular Virology and Howard Hughes Medical Institute, University of Wisconsin, Madison, WI 53706, USA
    Proc Natl Acad Sci U S A 100:15764-9. 2003
    ..The results further illuminate virus and cell pathways. Further refinement of virus screening likely will reveal contributions from additional host genes...
  22. pmc Host factors in positive-strand RNA virus genome replication
    Paul Ahlquist
    Institute for Molecular Virology, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    J Virol 77:8181-6. 2003
  23. ncbi request reprint Detecting differential gene expression with a semiparametric hierarchical mixture method
    Michael A Newton
    Department of Statistics, University of Wisconsin Madison, 1210 West Dayton St, Madison, WI 53706 1685, USA
    Biostatistics 5:155-76. 2004
    ..Compared to several competing methodologies, the proposed methodology exhibits good operating characteristics in a simulation study, on the analysis of spike-in data, and in a cross-validation calculation...
  24. pmc Production of infectious human papillomavirus independently of viral replication and epithelial cell differentiation
    Dohun Pyeon
    McArdle Laboratory for Cancer Research, Institute for Molecular Virology, University of Wisconsin, Madison, WI 53706, USA
    Proc Natl Acad Sci U S A 102:9311-6. 2005
    ....
  25. pmc Involvement of host cellular multivesicular body functions in hepatitis B virus budding
    Tokiko Watanabe
    Institute for Molecular Virology, McArdle Laboratory for Cancer Research, University of Wisconsin, 1525 Linden Drive, Madison, WI 53706, USA
    Proc Natl Acad Sci U S A 104:10205-10. 2007
    ..Moreover, HBV enveloped virions, enveloped subviral particles, and unenveloped nucleocapsids are all released by distinct pathways with separate host factor requirements...
  26. pmc Engineered retargeting of viral RNA replication complexes to an alternative intracellular membrane
    David J Miller
    Department of Medicine, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    J Virol 77:12193-202. 2003
    ..We conclude that specific intracellular membranes are not required for FHV RNA replication complex formation and function...
  27. pmc Nodavirus RNA replication protein a induces membrane association of genomic RNA
    Priscilla M Van Wynsberghe
    Institute for Molecular Virology, University of Wisconsin Madison, 1525 Linden Drive, Madison, WI 53706 1596, USA
    J Virol 81:4633-44. 2007
    ..These and other results show that prior to negative-strand RNA synthesis, multiple domains of mitochondrially targeted protein A cooperate to selectively recruit FHV genomic RNA to membranes where RNA replication complexes form...
  28. ncbi request reprint A positive-strand RNA virus replication complex parallels form and function of retrovirus capsids
    Michael Schwartz
    Institute for Molecular Virology and Howard Hughes Medical Institute, University of Wisconsin, Madison, Madison, WI 53706, USA
    Mol Cell 9:505-14. 2002
    ....
  29. pmc Cytoplasmic viral replication complexes
    Johan A den Boon
    Institute for Molecular Virology, University of Wisconsin Madison, Madison, WI 53706, USA
    Cell Host Microbe 8:77-85. 2010
    ....
  30. pmc Establishment of human papillomavirus infection requires cell cycle progression
    Dohun Pyeon
    McArdle Laboratory for Cancer Research, University of Wisconsin Madison, Madison, WI, USA
    PLoS Pathog 5:e1000318. 2009
    ..By defining a major condition for cell susceptibility to HPV infection, these results also have potentially important implications for HPV control...
  31. pmc 5' cis elements direct nodavirus RNA1 recruitment to mitochondrial sites of replication complex formation
    Priscilla M Van Wynsberghe
    Institute for Molecular Virology, University of Wisconsin Madison, Madison, Wisconsin 53706 1596, USA
    J Virol 83:2976-88. 2009
    ..These results establish RNA recruitment to the sites of replication complex formation as an essential, distinct, and selective early step in nodavirus replication...
  32. pmc Three-dimensional analysis of a viral RNA replication complex reveals a virus-induced mini-organelle
    Benjamin G Kopek
    Institute for Molecular Virology, University of Wisconsin Madison, Madison, Wisconsin, United States of America
    PLoS Biol 5:e220. 2007
    ..The resulting insights appear relevant to many other positive-strand RNA viruses and support recently proposed structural and likely evolutionary parallels with retrovirus and double-stranded RNA virus virions...
  33. pmc Fundamental differences in cell cycle deregulation in human papillomavirus-positive and human papillomavirus-negative head/neck and cervical cancers
    Dohun Pyeon
    McArdle Laboratory for Cancer Research, University of Wisconsin Madison, Madison, WI 53706, USA
    Cancer Res 67:4605-19. 2007
    ....
  34. ncbi request reprint High-throughput isolation of Saccharomyces cerevisiae RNA
    Billy T Dye
    University of Wisconsin Madison, Madison, WI, USA
    Biotechniques 38:868, 870. 2005
  35. pmc In vivo self-interaction of nodavirus RNA replicase protein a revealed by fluorescence resonance energy transfer
    Billy T Dye
    Howard Hughes Medical Institute, University of Wisconsin Madison, Madison, WI 53706, USA
    J Virol 79:8909-19. 2005
    ..Thus, the results imply that FHV protein A functions as a multimer rather than as a monomer at one or more steps in RNA replication...
  36. doi request reprint Filling a GAP(DH) in asymmetric viral RNA synthesis
    Xiaofeng Wang
    Institute for Molecular Virology, University of Wisconsin, Madison, WI 53706, USA
    Cell Host Microbe 3:124-5. 2008
    ..GAPDH binds TBSV (-)RNA and promotes the normal excess of (+)RNA over (-)RNA products, possibly by selectively retaining (-)RNA templates for copying...
  37. pmc Cellular transcription factor ZASC1 regulates murine leukemia virus transcription
    James W Bruce
    Institute for Molecular Virology, University of Wisconsin, Madison, Wisconsin 53706, USA
    J Virol 84:7473-83. 2010
    ..These studies identify the cellular transcription factor ZASC1 as an activator of MLV gene expression and provide tools that should be useful in studying the links between ZASC1 and human diseases...
  38. pmc Statistical use of argonaute expression and RISC assembly in microRNA target identification
    Stephen A Stanhope
    Department of Statistics, University of Wisconsin Madison, Madison, Wisconsin, USA
    PLoS Comput Biol 5:e1000516. 2009
    ....
  39. pmc Flock house virus RNA polymerase is a transmembrane protein with amino-terminal sequences sufficient for mitochondrial localization and membrane insertion
    David J Miller
    University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    J Virol 76:9856-67. 2002
    ..We conclude that protein A targets and anchors FHV RNA replication complexes to outer mitochondrial membranes, in part through an N-proximal mitochondrial localization signal and transmembrane domain...
  40. pmc Isolation of cell lines that show novel, murine leukemia virus-specific blocks to early steps of retroviral replication
    James W Bruce
    Institute for Molecular Virology, University of Wisconsin, Madison, 53706 1596, USA
    J Virol 79:12969-78. 2005
    ..These cell lines are important tools for the biochemical and genetic analysis of early stages in retrovirus infection...
  41. ncbi request reprint Genome-wide expression profiling reveals EBV-associated inhibition of MHC class I expression in nasopharyngeal carcinoma
    Srikumar Sengupta
    Institute for Molecular Virology, McArdle Laboratory for Cancer Research, University of Wisconsin Madison, Madison, WI 53706, USA
    Cancer Res 66:7999-8006. 2006
    ....
  42. pmc Intersection of the multivesicular body pathway and lipid homeostasis in RNA replication by a positive-strand RNA virus
    Xiaofeng Wang
    Institute for Molecular Virology, University of Wisconsin, Madison, WI 53706, USA
    J Virol 85:5494-503. 2011
    ..Among other points, these findings show the potential for blocking viral RNA replication by modulating lipid synthesis at multiple levels...
  43. ncbi request reprint Detecting protein-protein interaction in live yeast by flow cytometry
    Billy T Dye
    Howard Hughes Medical Institute, University of Wisconsin, 1525 Linden Drive, Madison, WI 53706, USA
    Cytometry A 63:77-86. 2005
    ..Probability binning is also employed to provide a statistical confirmation of our observations...
  44. pmc MicroRNA 29c is down-regulated in nasopharyngeal carcinomas, up-regulating mRNAs encoding extracellular matrix proteins
    Srikumar Sengupta
    Institute for Molecular Virology, Howard Hughes Medical Institute, McArdle Laboratory for Cancer Research, Departments of Statistics and Biostatistics and Medical Informatics, University of Wisconsin, Madison, WI 53076, USA
    Proc Natl Acad Sci U S A 105:5874-8. 2008
    ..Thus, we identify eight miRNAs differentially expressed in NPC and demonstrate the involvement of one in regulating genes involved in metastasis...
  45. pmc Drosophila RNAi screen identifies host genes important for influenza virus replication
    Linhui Hao
    Institute for Molecular Virology, USA
    Nature 454:890-3. 2008
    ....

Research Grants17

  1. GENE FUNCTIONS AND MOLECULAR GENETICS OF RNA VIRUSES
    Paul Ahlquist; Fiscal Year: 2009
    ..The results will advance understanding and control of widespread viral diseases, and understanding of normal cell functions relevant to other disease processes. ..
  2. Novel Approaches to Detect Virus-Cancer Associations
    Paul Ahlquist; Fiscal Year: 2005
    ..The methods developed also will be applicable to probe for virus involvement in other chronic or acute disorders. ..
  3. Novel Approaches to Detect Virus-Cancer Associations
    Paul Ahlquist; Fiscal Year: 2004
    ..The methods developed also will be applicable to probe for virus involvement in other chronic or acute disorders. ..
  4. Novel Approaches to Detect Virus-Cancer Associations
    Paul Ahlquist; Fiscal Year: 2003
    ..The methods developed also will be applicable to probe for virus involvement in other chronic or acute disorders. ..
  5. Novel Approaches to Detect Virus-Cancer Associations
    Paul Ahlquist; Fiscal Year: 2002
    ..The methods developed also will be applicable to probe for virus involvement in other chronic or acute disorders. ..
  6. GENE FUNCTIONS AND MOLECULAR GENETICS OF RNA VIRUSES
    Paul Ahlquist; Fiscal Year: 1992
    ....
  7. GENE FUNCTIONS AND MOLECULAR GENETICS OF RNA VIRUSES
    Paul Ahlquist; Fiscal Year: 1991
    ....
  8. GENE FUNCTIONS AND MOLECULAR GENETICS OF RNA VIRUSES
    Paul Ahlquist; Fiscal Year: 1990
    ....
  9. GENE FUNCTIONS AND MOLECULAR GENETICS OF RNA VIRUSES
    Paul Ahlquist; Fiscal Year: 2006
    ..The results will advance understanding and control of widespread viral diseases, and understanding of normal cell functions relevant to other disease processes. ..
  10. GENE FUNCTIONS AND MOLECULAR GENETICS OF RNA VIRUSES
    Paul Ahlquist; Fiscal Year: 2007
    ..The results will advance understanding and control of widespread viral diseases, and understanding of normal cell functions relevant to other disease processes. ..
  11. GENE FUNCTIONS AND MOLECULAR GENETICS OF RNA VIRUSES
    Paul Ahlquist; Fiscal Year: 1993
    ....