Xin Xiang

Summary

Affiliation: Uniformed Services University of the Health Sciences
Country: USA

Publications

  1. ncbi request reprint Hyphal tip growth and nuclear migration
    X Xiang
    Department of Biochemistry, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA
    Curr Opin Microbiol 2:636-40. 1999
  2. ncbi request reprint Nuclear migration and positioning in filamentous fungi
    Xin Xiang
    Department of Biochemistry and Molecular Biology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA
    Fungal Genet Biol 41:411-9. 2004
  3. ncbi request reprint Cytoskeleton and motor proteins in filamentous fungi
    Xin Xiang
    Department of Biochemistry and Molecular Biology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA
    Curr Opin Microbiol 6:628-33. 2003
  4. pmc A +TIP for a smooth trip
    Xin Xiang
    Department of Biochemistry and Molecular Biology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA
    J Cell Biol 172:651-4. 2006
  5. pmc LIS1 at the microtubule plus end and its role in dynein-mediated nuclear migration
    Xin Xiang
    Department of Biochemistry and Molecular Biology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA
    J Cell Biol 160:289-90. 2003
  6. ncbi request reprint Dynamics of cytoplasmic dynein in living cells and the effect of a mutation in the dynactin complex actin-related protein Arp1
    X Xiang
    Department of Biochemistry and Molecular Biology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA
    Curr Biol 10:603-6. 2000
  7. pmc Aspergillus myosin-V supports polarized growth in the absence of microtubule-based transport
    Jun Zhang
    Department of Biochemistry and Molecular Biology, Uniformed Services University of the Health Sciences, F Edward Hebert School of Medicine, Bethesda, Maryland, United States of America
    PLoS ONE 6:e28575. 2011
  8. pmc Accumulation of cytoplasmic dynein and dynactin at microtubule plus ends in Aspergillus nidulans is kinesin dependent
    Jun Zhang
    Department of Biochemistry and Molecular Biology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814, USA
    Mol Biol Cell 14:1479-88. 2003
  9. pmc Arp11 affects dynein-dynactin interaction and is essential for dynein function in Aspergillus nidulans
    Jun Zhang
    Department of Biochemistry and Molecular Biology, F Edward Hebert School of Medicine, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA
    Traffic 9:1073-87. 2008
  10. pmc Cytoplasmic dynein's mitotic spindle pole localization requires a functional anaphase-promoting complex, gamma-tubulin, and NUDF/LIS1 in Aspergillus nidulans
    Shihe Li
    Department of Biochemistry and Molecular Biology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA
    Mol Biol Cell 16:3591-605. 2005

Research Grants

  1. In vivo Regulation of Cytoplasmic Dynein
    Xin Xiang; Fiscal Year: 2007
  2. In vivo Regulation of Cytoplasmic Dynein
    Xin Xiang; Fiscal Year: 2009

Collaborators

Detail Information

Publications25

  1. ncbi request reprint Hyphal tip growth and nuclear migration
    X Xiang
    Department of Biochemistry, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA
    Curr Opin Microbiol 2:636-40. 1999
    ..Genes involved in nuclear migration continue to be identified, including putative regulators. The role of microtubules and microtubule motor proteins in hyphal tip growth has also been studied...
  2. ncbi request reprint Nuclear migration and positioning in filamentous fungi
    Xin Xiang
    Department of Biochemistry and Molecular Biology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA
    Fungal Genet Biol 41:411-9. 2004
    ..Here we review these studies and also present the need to further dissect how dynein and its associated microtubule cytoskeleton are involved mechanistically in nuclear positioning in the multinucleated hyphae...
  3. ncbi request reprint Cytoskeleton and motor proteins in filamentous fungi
    Xin Xiang
    Department of Biochemistry and Molecular Biology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA
    Curr Opin Microbiol 6:628-33. 2003
    ....
  4. pmc A +TIP for a smooth trip
    Xin Xiang
    Department of Biochemistry and Molecular Biology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA
    J Cell Biol 172:651-4. 2006
    ..e., to switch the depolymerizing end to a polymerizing end. The possibility that other +TIPs may play a similar role in sustaining a microtubule track during vesicular transport deserves investigation...
  5. pmc LIS1 at the microtubule plus end and its role in dynein-mediated nuclear migration
    Xin Xiang
    Department of Biochemistry and Molecular Biology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA
    J Cell Biol 160:289-90. 2003
    ..In this issue, Lee et al. (2003) describe important new findings in Saccharomyces cerevisiae regarding the role of LIS1 (Pac1) in dynein-mediated nuclear migration...
  6. ncbi request reprint Dynamics of cytoplasmic dynein in living cells and the effect of a mutation in the dynactin complex actin-related protein Arp1
    X Xiang
    Department of Biochemistry and Molecular Biology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA
    Curr Biol 10:603-6. 2000
    ..A mutation in actin-related protein Arp1 of the dynactin complex abolishes the presence of these dynamic GFP-NUDA structures near the hyphal tip, suggesting a targeting role of the dynactin complex...
  7. pmc Aspergillus myosin-V supports polarized growth in the absence of microtubule-based transport
    Jun Zhang
    Department of Biochemistry and Molecular Biology, Uniformed Services University of the Health Sciences, F Edward Hebert School of Medicine, Bethesda, Maryland, United States of America
    PLoS ONE 6:e28575. 2011
    ..Finally, our genetic analyses also indicate that KINA (kinesin-1) rather than UNCA (kinesin-3) is the major kinesin motor that supports polarized growth in the absence of MYOV...
  8. pmc Accumulation of cytoplasmic dynein and dynactin at microtubule plus ends in Aspergillus nidulans is kinesin dependent
    Jun Zhang
    Department of Biochemistry and Molecular Biology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814, USA
    Mol Biol Cell 14:1479-88. 2003
    ..Finally, we demonstrate that a conventional kinesin, KINA, is required for the microtubule plus-end accumulation of cytoplasmic dynein and dynactin, but not of NUDF...
  9. pmc Arp11 affects dynein-dynactin interaction and is essential for dynein function in Aspergillus nidulans
    Jun Zhang
    Department of Biochemistry and Molecular Biology, F Edward Hebert School of Medicine, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA
    Traffic 9:1073-87. 2008
    ..We suggest that loss of the pointed-end proteins alters the Arp1 filament in a way that affects the conformation of p150 required for its proper interaction with the dynein motor...
  10. pmc Cytoplasmic dynein's mitotic spindle pole localization requires a functional anaphase-promoting complex, gamma-tubulin, and NUDF/LIS1 in Aspergillus nidulans
    Shihe Li
    Department of Biochemistry and Molecular Biology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA
    Mol Biol Cell 16:3591-605. 2005
    ....
  11. pmc Identification of a novel site in the tail of dynein heavy chain important for dynein function in vivo
    Rongde Qiu
    Department of Biochemistry and Molecular Biology, The Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814, USA
    J Biol Chem 288:2271-80. 2013
    ....
  12. pmc Dynein light intermediate chain in Aspergillus nidulans is essential for the interaction between heavy and intermediate chains
    Jun Zhang
    From the Department of Biochemistry and Molecular Biology, The Uniformed Services University of the Health Sciences F Edward Hébert School of Medicine, Bethesda, Maryland 20814, USA
    J Biol Chem 284:34760-8. 2009
    ..Without a functional LIC, the HC-IC association is significantly weakened, and the HCs could no longer accumulate at the microtubule plus end. Thus, the LIC is essential for the assembly of the core complex of dynein in Aspergillus...
  13. pmc The p25 subunit of the dynactin complex is required for dynein-early endosome interaction
    Jun Zhang
    Department of Biochemistry and Molecular Biology, The Uniformed Services University, Bethesda, MD 20814, USA
    J Cell Biol 193:1245-55. 2011
    ..These results indicate that p25, in conjunction with the rest of the dynactin complex, is important for dynein-early endosome interaction...
  14. doi request reprint Nuclear positioning: dynein needed for microtubule shrinkage-coupled movement
    Xin Xiang
    Department of Biochemistry and Molecular Biology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA
    Curr Biol 22:R496-9. 2012
    ..A new study has revealed a novel protein interaction linking microtubule plus-ends with the cortex and a role for dynein in microtubule shrinkage-coupled movement...
  15. pmc In vivo roles of the basic domain of dynactin p150 in microtubule plus-end tracking and dynein function
    Xuanli Yao
    Department of Biochemistry and Molecular Biology, The Uniformed Services University, Bethesda, MD, USA
    Traffic 13:375-87. 2012
    ..We suggest that the basic amino acids are required for the electrostatic interaction between p150 and MTs, which is important for kinesin-1-mediated plus-end targeting of dynactin and dynein in A. nidulans...
  16. ncbi request reprint Motor proteins at the microtubule plus-end
    Xufeng Wu
    Laboratory of Cell Biology, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Trends Cell Biol 16:135-43. 2006
    ....
  17. pmc Point mutations in the stem region and the fourth AAA domain of cytoplasmic dynein heavy chain partially suppress the phenotype of NUDF/LIS1 loss in Aspergillus nidulans
    Lei Zhuang
    Department of Biochemistry and Molecular Biology, Uniformed Services University of the Health Sciences F Edward Hébert School of Medicine, Bethesda, Maryland 20814, USA
    Genetics 175:1185-96. 2007
    ..The fact that a mutation in the end of AAA4 negatively regulates dynein's ATPase activity but partially compensates for NUDF loss indicates the importance of the AAA4 domain in dynein regulation in vivo...
  18. ncbi request reprint Cytoplasmic dynein intermediate chain and heavy chain are dependent upon each other for microtubule end localization in Aspergillus nidulans
    Jun Zhang
    Department of Biochemistry and Molecular Biology, USUHS, Bethesda, MD 20814, USA
    Mol Microbiol 44:381-92. 2002
    ....
  19. pmc The microtubule plus-end localization of Aspergillus dynein is important for dynein-early-endosome interaction but not for dynein ATPase activation
    Jun Zhang
    Department of Biochemistry and Molecular Biology, USUHS, Bethesda, MD 20814, USA
    J Cell Sci 123:3596-604. 2010
    ..We suggest that the normal ability of dyenin to interact with microtubules as an active minus-end-directed motor demands kinesin-1-mediated plus-end accumulation for effective interactions with early endosomes...
  20. ncbi request reprint The Aspergillus cytoplasmic dynein heavy chain and NUDF localize to microtubule ends and affect microtubule dynamics
    G Han
    Department of Biochemistry and Molecular Biology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA
    Curr Biol 11:719-24. 2001
    ..The microtubules in the mutant cells also paused longer at the hyphal tip than wild-type microtubules. These results indicate that cytoplasmic dynein and the LIS1 homolog NUDF affect microtubule dynamics in vivo...
  21. pmc Dual-Color imaging of nuclear division and mitotic spindle elongation in live cells of Aspergillus nidulans
    Wenqi Su
    Department of Biochemistry and Molecular Biology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814, USA
    Eukaryot Cell 3:553-6. 2004
    ..This system allows new details of mitosis and nuclear movement to be revealed...
  22. pmc Establishing a novel knock-in mouse line for studying neuronal cytoplasmic dynein under normal and pathologic conditions
    Jun Zhang
    Department of Biochemistry and Molecular Biology, The Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814, USA
    Cytoskeleton (Hoboken) 70:215-27. 2013
    ..Thus, we have created a useful tool for studying dynein function in the central nervous system under normal and pathologic conditions...
  23. pmc Spindle formation in Aspergillus is coupled to tubulin movement into the nucleus
    Yulia Ovechkina
    Department of Molecular Genetics, The Ohio State University, Columbus 43210, USA
    Mol Biol Cell 14:2192-200. 2003
    ..nidulans. They suggest that regulation of protein movement into the nucleoplasm may be important for regulating mitotic onset in organisms with intranuclear mitosis...
  24. ncbi request reprint The requirement of the LC8 dynein light chain for nuclear migration and septum positioning is temperature dependent in Aspergillus nidulans
    Bo Liu
    Section of Plant Biology, University of California, One Shields Avenue, Davis, CA 95616, USA
    Mol Microbiol 47:291-301. 2003
    ..Such localizations were absent at 42 degrees C in mutant cells, but not in control cells. We conclude that LC8 plays a role in DHC localization/function, and the requirement for such a role in A. nidulans cells is temperature dependent...
  25. pmc CLIP-170 homologue and NUDE play overlapping roles in NUDF localization in Aspergillus nidulans
    Vladimir P Efimov
    Department of Pharmacology, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
    Mol Biol Cell 17:2021-34. 2006
    ..Although the KipA kinesin (Kip2/Tea2 homologue) did not affect plus-end localization of CLIPA at 32 degrees C, it was required for enhancing plus-end accumulation of CLIPA at an elevated temperature (42 degrees C)...

Research Grants5

  1. In vivo Regulation of Cytoplasmic Dynein
    Xin Xiang; Fiscal Year: 2007
    ..Our third aim is to create null-mutants of several proteins in the dynein complex to study their functions in dynein regulation, and identify novel dynein regulators by cloning additional nud genes. ..
  2. In vivo Regulation of Cytoplasmic Dynein
    Xin Xiang; Fiscal Year: 2009
    ..Our third aim is to create null-mutants of several proteins in the dynein complex to study their functions in dynein regulation, and identify novel dynein regulators by cloning additional nud genes. ..