David Horowitz

Summary

Affiliation: Uniformed Services University of the Health Sciences
Country: USA

Publications

  1. doi request reprint The mechanism of the second step of pre-mRNA splicing
    David S Horowitz
    Department of Biochemistry and Molecular Biology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA
    Wiley Interdiscip Rev RNA 3:331-50. 2012
  2. pmc A cyclophilin functions in pre-mRNA splicing
    David S Horowitz
    Department of Biochemistry and Molecular Biology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA
    EMBO J 21:470-80. 2002
  3. pmc The splice is right: guarantors of fidelity in pre-mRNA splicing
    David S Horowitz
    Department of Biochemistry and Molecular Biology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814, USA
    RNA 17:551-4. 2011
  4. pmc The Prp18 protein stabilizes the interaction of both exons with the U5 snRNA during the second step of pre-mRNA splicing
    Luciana B Crotti
    Department of Biochemistry and Molecular Biology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814, USA
    Genes Dev 21:1204-16. 2007
  5. pmc Mutational analysis identifies two separable roles of the Saccharomyces cerevisiae splicing factor Prp18
    Dagmar Bacikova
    Department of Biochemistry, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814, USA
    RNA 8:1280-93. 2002
  6. pmc Genetic and functional interaction of evolutionarily conserved regions of the Prp18 protein and the U5 snRNA
    Dagmar Bacikova
    Department of Biochemistry and Molecular Biology, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Rd, Bethesda, MD 20814, USA
    Mol Cell Biol 25:2107-16. 2005
  7. pmc Exon sequences at the splice junctions affect splicing fidelity and alternative splicing
    Luciana B Crotti
    Department of Biochemistry and Molecular Biology, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Rd, Bethesda, MD 20814, USA
    Proc Natl Acad Sci U S A 106:18954-9. 2009
  8. ncbi request reprint Crystal structure of a complex between human spliceosomal cyclophilin H and a U4/U6 snRNP-60K peptide
    Ulrich Reidt
    Abteilung für Zelluläre Biochemie, Max Planck Institut fur biophysikalische Chemie, 37077 Gottingen, Germany
    J Mol Biol 331:45-56. 2003
  9. pmc Functional interactions between Prp8, Prp18, Slu7, and U5 snRNA during the second step of pre-mRNA splicing
    Anna Aronova
    Department of Microbiology and Immunology, Weill Cornell Medical College, New York, NY 10021, USA
    RNA 13:1437-44. 2007

Research Grants

  1. BIOCHEMISTRY OF PREMRNA SPLICING FACTORS
    David Horowitz; Fiscal Year: 2004

Collaborators

  • T Misteli
  • Luciana B Crotti
  • Dagmar Bacikova
  • Anna Aronova
  • Ulrich Reidt
  • Beate Schwer
  • Dirk Fasshauer
  • Ralf Ficner
  • Markus C Wahl
  • Reinhard Luhrmann

Detail Information

Publications9

  1. doi request reprint The mechanism of the second step of pre-mRNA splicing
    David S Horowitz
    Department of Biochemistry and Molecular Biology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA
    Wiley Interdiscip Rev RNA 3:331-50. 2012
    ..The sequence of events in the second step is presented, focusing on the actions of the proteins in setting up and facilitating the second reaction. Mechanisms for avoiding errors in splicing are discussed...
  2. pmc A cyclophilin functions in pre-mRNA splicing
    David S Horowitz
    Department of Biochemistry and Molecular Biology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA
    EMBO J 21:470-80. 2002
    ..These results provide an example of the function of a cyclophilin in a complex process and provide insight into the mechanisms of action of cyclophilins...
  3. pmc The splice is right: guarantors of fidelity in pre-mRNA splicing
    David S Horowitz
    Department of Biochemistry and Molecular Biology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814, USA
    RNA 17:551-4. 2011
    ..The discussion here reviews and integrates the results of the two papers and describes the implications for proofreading in splicing...
  4. pmc The Prp18 protein stabilizes the interaction of both exons with the U5 snRNA during the second step of pre-mRNA splicing
    Luciana B Crotti
    Department of Biochemistry and Molecular Biology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814, USA
    Genes Dev 21:1204-16. 2007
    ..Our results show that As are preferred at the ends of both exons and support a revised model of the interactions of the exons with U5 in which the exons are arranged in a continuous double helix that facilitates the second reaction...
  5. pmc Mutational analysis identifies two separable roles of the Saccharomyces cerevisiae splicing factor Prp18
    Dagmar Bacikova
    Department of Biochemistry, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814, USA
    RNA 8:1280-93. 2002
    ..Prp18 thus appears to have two separable roles in splicing, one in stabilizing interaction of Slu7 with the spliceosome, and a second that requires the conserved loop...
  6. pmc Genetic and functional interaction of evolutionarily conserved regions of the Prp18 protein and the U5 snRNA
    Dagmar Bacikova
    Department of Biochemistry and Molecular Biology, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Rd, Bethesda, MD 20814, USA
    Mol Cell Biol 25:2107-16. 2005
    ....
  7. pmc Exon sequences at the splice junctions affect splicing fidelity and alternative splicing
    Luciana B Crotti
    Department of Biochemistry and Molecular Biology, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Rd, Bethesda, MD 20814, USA
    Proc Natl Acad Sci U S A 106:18954-9. 2009
    ..Finally, we note that the degeneracy of the genetic code allows competing 3' splice sites to be eliminated from coding regions, and we suggest that the evolution of the splicing signals and the genetic code are connected...
  8. ncbi request reprint Crystal structure of a complex between human spliceosomal cyclophilin H and a U4/U6 snRNP-60K peptide
    Ulrich Reidt
    Abteilung für Zelluläre Biochemie, Max Planck Institut fur biophysikalische Chemie, 37077 Gottingen, Germany
    J Mol Biol 331:45-56. 2003
    ....
  9. pmc Functional interactions between Prp8, Prp18, Slu7, and U5 snRNA during the second step of pre-mRNA splicing
    Anna Aronova
    Department of Microbiology and Immunology, Weill Cornell Medical College, New York, NY 10021, USA
    RNA 13:1437-44. 2007
    ....

Research Grants5

  1. BIOCHEMISTRY OF PREMRNA SPLICING FACTORS
    David Horowitz; Fiscal Year: 2004
    ..Following these interactions through the second step of the reaction will further the understanding of the mechanism of splicing. ..