William F Blakely

Summary

Affiliation: Uniformed Services University of the Health Sciences
Country: USA

Publications

  1. ncbi request reprint Induction of premature chromosome condensation by a phosphatase inhibitor and a protein kinase in unstimulated human peripheral blood lymphocytes: a simple and rapid technique to study chromosome aberrations using specific whole-chromosome DNA hybridizati
    P G Prasanna
    Applied Cellular Radiobiology Department, Armed Forces Radiobiology Research Institute, 8901 Wisconsin Avenue, Bethesda, MD 20889 5603, USA
    Mutat Res 466:131-41. 2000
  2. ncbi request reprint Real-time quantitative RT-PCR assay of GADD45 gene expression changes as a biomarker for radiation biodosimetry
    M B Grace
    Biological Dosimetry Team, Armed Forces Radiobiology Research Institute, 8901 Wisconsin Avenue, Bethesda, MD 20889 5603, USA
    Int J Radiat Biol 78:1011-21. 2002
  3. ncbi request reprint Early-response biological dosimetry--recommended countermeasure enhancements for mass-casualty radiological incidents and terrorism
    William F Blakely
    Biological Dosimetry Team, Armed Forces Radiobiology Research Institute, Uniformed Services University of the Health Sciences, 8901 Wisconsin Avenue, Bethesda, MD 20889 5603, USA
    Health Phys 89:494-504. 2005
  4. ncbi request reprint Radiation exposure assessment using cytological and molecular biomarkers
    W F Blakely
    AFRRI ACR 8901 Wisconsin Avenue Bethesda, MD 20889 5603, USA
    Radiat Prot Dosimetry 97:17-23. 2001
  5. ncbi request reprint Radiation biodosimetry: applications for spaceflight
    W F Blakely
    Armed Forces Radiobiology Research Institute AFRRI, Bethesda, MD 20889 5603, USA
    Adv Space Res 31:1487-93. 2003
  6. doi request reprint Biodosimetry medical recording-use of the Biodosimetry Assessment Tool
    William F Blakely
    Scientific Research Department, Armed Forces Radiobiology Research Institute AFRRI, Bethesda, MD 20889 5603, USA
    Health Phys 99:S184-91. 2010
  7. ncbi request reprint Multiple blood-proteins approach for early-response exposure assessment using an in vivo murine radiation model
    Natalia I Ossetrova
    Uniformed Services University, Armed Forces Radiobiology Research Institute, Scientific Research Department, Bethesda, Maryland 20889 5603, USA
    Int J Radiat Biol 85:837-50. 2009
  8. doi request reprint Multiple parameter radiation injury assessment using a nonhuman primate radiation model-biodosimetry applications
    William F Blakely
    Armed Forces Radiobiology Research Institute, 8901 Wisconsin Avenue, Bethesda, MD 20889 5603 USA
    Health Phys 98:153-9. 2010
  9. doi request reprint Optimization of calyculin A-induced premature chromosome condensation assay for chromosome aberration studies
    Tomisato Miura
    Scientific Research Department, Armed Forces Radiobiology Research Institute AFRRI, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20889, USA
    Cytometry A 79:1016-22. 2011
  10. doi request reprint Combined approach of hematological biomarkers and plasma protein SAA for improvement of radiation dose assessment triage in biodosimetry applications
    Natalia I Ossetrova
    AFRRI, SRD, 8901 Wisconsin Avenue, Bethesda, MD 20889 5603, USA
    Health Phys 98:204-8. 2010

Detail Information

Publications28

  1. ncbi request reprint Induction of premature chromosome condensation by a phosphatase inhibitor and a protein kinase in unstimulated human peripheral blood lymphocytes: a simple and rapid technique to study chromosome aberrations using specific whole-chromosome DNA hybridizati
    P G Prasanna
    Applied Cellular Radiobiology Department, Armed Forces Radiobiology Research Institute, 8901 Wisconsin Avenue, Bethesda, MD 20889 5603, USA
    Mutat Res 466:131-41. 2000
    ..966] and a nonlinear power [Y=(5.70+/-0.46)D((0.61+/-0.05)), r(2)=0.9901) model. This technique can be applied to biological dosimetry of radiation exposures involving uniform whole-body low linear energy transfer (LET) exposures...
  2. ncbi request reprint Real-time quantitative RT-PCR assay of GADD45 gene expression changes as a biomarker for radiation biodosimetry
    M B Grace
    Biological Dosimetry Team, Armed Forces Radiobiology Research Institute, 8901 Wisconsin Avenue, Bethesda, MD 20889 5603, USA
    Int J Radiat Biol 78:1011-21. 2002
    ..To assess the efficacy of fluorescent-based quantitative reverse transcription-polymerase chain reaction (QRT-PCR) technology to measure gene expression changes (GEC) for rapid, point-of-care radiation dose assessment...
  3. ncbi request reprint Early-response biological dosimetry--recommended countermeasure enhancements for mass-casualty radiological incidents and terrorism
    William F Blakely
    Biological Dosimetry Team, Armed Forces Radiobiology Research Institute, Uniformed Services University of the Health Sciences, 8901 Wisconsin Avenue, Bethesda, MD 20889 5603, USA
    Health Phys 89:494-504. 2005
    ..These research and applied science efforts should ultimately contribute towards approved, regulated biodosimetry devices or diagnostic tests integrated into a national radioprotection program...
  4. ncbi request reprint Radiation exposure assessment using cytological and molecular biomarkers
    W F Blakely
    AFRRI ACR 8901 Wisconsin Avenue Bethesda, MD 20889 5603, USA
    Radiat Prot Dosimetry 97:17-23. 2001
    ..The new methodologies that have a number of other applications, together with diagnostic software now in development, could improve the United States military's emergency response capability and medical readiness...
  5. ncbi request reprint Radiation biodosimetry: applications for spaceflight
    W F Blakely
    Armed Forces Radiobiology Research Institute AFRRI, Bethesda, MD 20889 5603, USA
    Adv Space Res 31:1487-93. 2003
    ..These radiation assessment diagnostic technologies can have dual-use applications supporting general medical-related care...
  6. doi request reprint Biodosimetry medical recording-use of the Biodosimetry Assessment Tool
    William F Blakely
    Scientific Research Department, Armed Forces Radiobiology Research Institute AFRRI, Bethesda, MD 20889 5603, USA
    Health Phys 99:S184-91. 2010
    ..The BAT report template is compliant with NATO and international guidance for recording ionizing radiation exposures for medical purposes...
  7. ncbi request reprint Multiple blood-proteins approach for early-response exposure assessment using an in vivo murine radiation model
    Natalia I Ossetrova
    Uniformed Services University, Armed Forces Radiobiology Research Institute, Scientific Research Department, Bethesda, Maryland 20889 5603, USA
    Int J Radiat Biol 85:837-50. 2009
    ..The aim was to evaluate the utility of multiple blood-protein biomarkers for early-response assessment of radiation exposure using a murine radiation model system...
  8. doi request reprint Multiple parameter radiation injury assessment using a nonhuman primate radiation model-biodosimetry applications
    William F Blakely
    Armed Forces Radiobiology Research Institute, 8901 Wisconsin Avenue, Bethesda, MD 20889 5603 USA
    Health Phys 98:153-9. 2010
    ..These results demonstrate the practical use of multiple parameter biomarkers to enhance the discrimination of exposed vs. non-exposed individuals and justify a follow-on rhesus macaque dose-response study...
  9. doi request reprint Optimization of calyculin A-induced premature chromosome condensation assay for chromosome aberration studies
    Tomisato Miura
    Scientific Research Department, Armed Forces Radiobiology Research Institute AFRRI, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20889, USA
    Cytometry A 79:1016-22. 2011
    ..Published 2011 Wiley Periodicals, Inc...
  10. doi request reprint Combined approach of hematological biomarkers and plasma protein SAA for improvement of radiation dose assessment triage in biodosimetry applications
    Natalia I Ossetrova
    AFRRI, SRD, 8901 Wisconsin Avenue, Bethesda, MD 20889 5603, USA
    Health Phys 98:204-8. 2010
    ....
  11. doi request reprint Synopsis of partial-body radiation diagnostic biomarkers and medical management of radiation injury workshop
    Pataje G S Prasanna
    Armed Forces Radiobiology Research Institute, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20889, USA
    Radiat Res 173:245-53. 2010
    ..For each area, the status, advantages and limitations of existing approaches and suggestions for new directions are presented...
  12. ncbi request reprint Molecular and cellular biology of moderate-dose (1-10 Gy) radiation and potential mechanisms of radiation protection: report of a workshop at Bethesda, Maryland, December 17-18, 2001
    C Norman Coleman
    Radiation Oncology Sciences Program, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland 20892, USA
    Radiat Res 159:812-34. 2003
    ..Communicate knowledge of the effects of radiation exposure to the general public and to investigators, policy makers and agencies involved in response to nuclear accidents/events and protection/treatment of the general public...
  13. ncbi request reprint Use of a centrifuge-based automated blood cell counter for radiation dose assessment
    Marcy B Grace
    Armed Forces Radiobiology Research Institute, Biological Dosimetry Team, Bethesda, MD 20889 5603, USA
    Mil Med 171:908-12. 2006
    ..This simple dry procedure provides reliable lymphocyte counts for results that fall within the normal lymphocyte count range (1-4 x 10(9) cells per L) for radiation exposure assessment using lymphocyte-depletion kinetics...
  14. ncbi request reprint Nucleic acid molecular biomarkers for diagnostic biodosimetry applications: use of the fluorogenic 5'-nuclease polymerase chain reaction assay
    William F Blakely
    Armed Forces Radiobiology Research Institute, 8901 Wisconsin Avenue, Bethesda, MD 20889 5603, USA
    Mil Med 167:16-9. 2002
    ..Our research shows that the analysis of multitarget nucleic acid biomarkers, using the multiplex fluorogenic 5'-nuclease PCR assay, has beneficial applications in radiation epidemiology, radiation therapy, and biodosimetry...
  15. pmc Radiation metabolomics. 5. Identification of urinary biomarkers of ionizing radiation exposure in nonhuman primates by mass spectrometry-based metabolomics
    Caroline H Johnson
    Laboratory of Metabolism, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA
    Radiat Res 178:328-40. 2012
    ....
  16. pmc The use of gamma-H2AX as a biodosimeter for total-body radiation exposure in non-human primates
    Christophe E Redon
    Laboratory of Molecular Pharmacology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA
    PLoS ONE 5:e15544. 2010
    ..However, knowledge of individual exposures is essential for early triage during radiological incidents to provide optimum possible life-sparing medical procedures to each person...
  17. ncbi request reprint Overview of low-level radiation exposure assessment: biodosimetry
    William F Blakely
    Armed Forces Radiobiology Research Institute, 8901 Wisconsin Avenue, Bethesda, MD 20889 5603, USA
    Mil Med 167:20-4. 2002
    ..Alternative real-time biomarkers that can be measured in field laboratories or with handheld detection devices show promise as screening and clinical diagnostic tools, but they require further development and validation studies...
  18. doi request reprint Biodosimetry Assessment Tool (BAT) software-dose prediction algorithms
    David J Sandgren
    Health Phys 99:S171-83. 2010
    ....
  19. ncbi request reprint Biodosimety Assessment Tool: a post-exposure software application for management of radiation accidents
    R C Sine
    Armed Forces Radiobiology Research Institute, Bethesda, MD 20889 5603, USA
    Mil Med 166:85-7. 2001
    ....
  20. ncbi request reprint Proto-oncogene expression: a predictive assay for radiation biodosimetry applications
    A C Miller
    Applied Cellular Radiobiology Department, Armed Forces Radiobiology Research Institute, 8901 Wisconsin Avenue, Bethesda, MD 20889 5603, USA
    Radiat Prot Dosimetry 99:295-302. 2002
    ..The use of real-time PCR technologies to quantify gene expression changes will also be discussed...
  21. ncbi request reprint Premature chromosome condensation in human resting peripheral blood lymphocytes for chromosome aberration analysis using specific whole-chromosome DNA hybridization probes
    Pataje G S Prasanna
    Armed Forces Radiobiology Research Institute, Bethesda, MD, USA
    Methods Mol Biol 291:49-57. 2005
    ..This method may be used in many biological and toxicological fields that require analysis of numerical and structural aberrations involving specific chromosomes...
  22. ncbi request reprint Biological dosimetry using human interphase peripheral blood lymphocytes
    Pataje G S Prasanna
    Applied Cellular Radiobiology Department, Armed Forces Radiobiology Research Institute, 8901 Wisconsin Avenue, Bethesda, MD 20889 5603, USA
    Mil Med 167:10-2. 2002
    ....
  23. ncbi request reprint Protein biomarkers for enhancement of radiation dose and injury assessment in nonhuman primate total-body irradiation model
    Natalia I Ossetrova
    Armed Forces Radiobiology Research Institute, 8901 Wisconsin Avenue, Bethesda, MD 20889 5693, USA
    Radiat Prot Dosimetry 159:61-76. 2014
    ..The combination of protein biomarkers provides greater accuracy for early radiation assessment than any one biomarker alone. ..
  24. ncbi request reprint Development and assessment of a quantitative reverse transcription-PCR assay for simultaneous measurement of four amplicons
    Marcy B Grace
    Armed Forces Radiobiology Research Institute, Biological Dosimetry Team, 8901 Wisconsin Ave, Bethesda, MD 20889 5603, USA
    Clin Chem 49:1467-75. 2003
    ....
  25. doi request reprint WHO 1st consultation on the development of a global biodosimetry laboratories network for radiation emergencies (BioDoseNet)
    William F Blakely
    Armed Forces Radiobiology Research Institute, Uniformed Services University, Bethesda, MD, USA
    Radiat Res 171:127-39. 2009
    ..Follow-on meetings are planned to further develop technical details for this network...
  26. doi request reprint NASA Radiation Biomarker Workshop, September 27-28, 2007
    Tore Straume
    NASA Ames Research Center, Moffett Field, California 94035, B Columbia University, New York, New York 10032, USA
    Radiat Res 170:393-405. 2008
    ..A summary of conclusions is provided at the end of the report...
  27. ncbi request reprint Multiple parameter biodosimetry of exposed workers from the JCO criticality accident in Tokai-mura
    William F Blakely
    J Radiol Prot 22:5-6. 2002
  28. ncbi request reprint Medical management of the acute radiation syndrome: recommendations of the Strategic National Stockpile Radiation Working Group
    Jamie K Waselenko
    Walter Reed Army Medical Center and Catholic University of America, Washington, DC 20307, USA
    Ann Intern Med 140:1037-51. 2004
    ..For terrorist or accidental events involving exposure to radioiodines, prophylaxis against malignant disease of the thyroid is also recommended, particularly for children and adolescents...