Hasan B Alam

Summary

Affiliation: Uniformed Services University of the Health Sciences
Country: USA

Publications

  1. ncbi request reprint Hypertonic saline: intraosseous infusion causes myonecrosis in a dehydrated swine model of uncontrolled hemorrhagic shock
    Hasan B Alam
    Department of Surgery, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814, USA
    J Trauma 52:18-25. 2002
  2. ncbi request reprint Hemorrhage control in the battlefield: role of new hemostatic agents
    Hasan B Alam
    Trauma Research and Readiness Institute for Surgery, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA
    Mil Med 170:63-9. 2005
  3. ncbi request reprint Application of a zeolite hemostatic agent achieves 100% survival in a lethal model of complex groin injury in Swine
    Hasan B Alam
    Department of Surgery, Uniformed Services University of the Health Sciences, Bethesda, Maryland, USA
    J Trauma 56:974-83. 2004
  4. ncbi request reprint Effect of different resuscitation strategies on neutrophil activation in a swine model of hemorrhagic shock
    Hasan B Alam
    Department of Surgery, Uniformed Services University of the Health Sciences, USUHS, Room A 3021, 4301 Jones Bridge Road, Bethesda, MD 20814, USA
    Resuscitation 60:91-9. 2004
  5. ncbi request reprint Combat casualty care research: from bench to the battlefield
    Hasan B Alam
    Trauma Research and Readiness Institute for Surgery, Department of Surgery, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814 4799, USA
    World J Surg 29:S7-11. 2005
  6. ncbi request reprint Profound hypothermia protects neurons and astrocytes, and preserves cognitive functions in a Swine model of lethal hemorrhage
    Hasan B Alam
    Trauma Research and Readiness Institute for Surgery, Bethesda, Maryland, USA
    J Surg Res 126:172-81. 2005
  7. ncbi request reprint Comparative analysis of hemostatic agents in a swine model of lethal groin injury
    Hasan B Alam
    Department of Surgery, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814, USA
    J Trauma 54:1077-82. 2003
  8. ncbi request reprint cDNA array analysis of gene expression following hemorrhagic shock and resuscitation in rats
    Hasan B Alam
    Department of Surgery, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, MD 20814, USA
    Resuscitation 54:195-206. 2002
  9. ncbi request reprint The rate of induction of hypothermic arrest determines the outcome in a Swine model of lethal hemorrhage
    Hasan B Alam
    Trauma Research and Readiness Institute for Surgery, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814, USA
    J Trauma 57:961-9. 2004
  10. doi request reprint Alterations in gene expression after induction of profound hypothermia for the treatment of lethal hemorrhage
    Hasan B Alam
    Division of Trauma, Department of Surgery, Massachusetts General Hospital Harvard Medical School, Boston, Massachusetts 02114, USA
    J Trauma 68:1084-98. 2010

Collaborators

Detail Information

Publications80

  1. ncbi request reprint Hypertonic saline: intraosseous infusion causes myonecrosis in a dehydrated swine model of uncontrolled hemorrhagic shock
    Hasan B Alam
    Department of Surgery, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814, USA
    J Trauma 52:18-25. 2002
    ..5% hypertonic saline (HTS) for combat casualties in shock. We tested the safety and efficacy of this recommendation in a long-term survival model of uncontrolled hemorrhagic shock using dehydrated swine...
  2. ncbi request reprint Hemorrhage control in the battlefield: role of new hemostatic agents
    Hasan B Alam
    Trauma Research and Readiness Institute for Surgery, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA
    Mil Med 170:63-9. 2005
    ..The purpose of this article is to summarize the background information regarding these hemostatic agents, indications and rationale for their use, and characteristics of these products that may impact effectiveness...
  3. ncbi request reprint Application of a zeolite hemostatic agent achieves 100% survival in a lethal model of complex groin injury in Swine
    Hasan B Alam
    Department of Surgery, Uniformed Services University of the Health Sciences, Bethesda, Maryland, USA
    J Trauma 56:974-83. 2004
    ..We tested ZH with increasing RM against controls and other hemostatic agents in a swine model of battlefield injury...
  4. ncbi request reprint Effect of different resuscitation strategies on neutrophil activation in a swine model of hemorrhagic shock
    Hasan B Alam
    Department of Surgery, Uniformed Services University of the Health Sciences, USUHS, Room A 3021, 4301 Jones Bridge Road, Bethesda, MD 20814, USA
    Resuscitation 60:91-9. 2004
    ..These findings suggest that the type of resuscitation fluid and method of infusion can influence neutrophil function...
  5. ncbi request reprint Combat casualty care research: from bench to the battlefield
    Hasan B Alam
    Trauma Research and Readiness Institute for Surgery, Department of Surgery, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814 4799, USA
    World J Surg 29:S7-11. 2005
    ..The impact of these basic science research findings on changes in military medical care and outcome of injured soldiers is also described...
  6. ncbi request reprint Profound hypothermia protects neurons and astrocytes, and preserves cognitive functions in a Swine model of lethal hemorrhage
    Hasan B Alam
    Trauma Research and Readiness Institute for Surgery, Bethesda, Maryland, USA
    J Surg Res 126:172-81. 2005
    ..Furthermore, our goal was to correlate the histological changes in the brain with neurological outcome, and the levels of circulating brain specific markers...
  7. ncbi request reprint Comparative analysis of hemostatic agents in a swine model of lethal groin injury
    Hasan B Alam
    Department of Surgery, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814, USA
    J Trauma 54:1077-82. 2003
    ..A large-animal model of lethal, uncontrolled hemorrhage was developed to test whether the use of various hemostatic agents would decrease bleeding and improve early survival...
  8. ncbi request reprint cDNA array analysis of gene expression following hemorrhagic shock and resuscitation in rats
    Hasan B Alam
    Department of Surgery, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, MD 20814, USA
    Resuscitation 54:195-206. 2002
    ..The aim of this study was to characterize gene expression following hemorrhagic shock and resuscitation with emphasis on the differences between various resuscitation strategies...
  9. ncbi request reprint The rate of induction of hypothermic arrest determines the outcome in a Swine model of lethal hemorrhage
    Hasan B Alam
    Trauma Research and Readiness Institute for Surgery, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814, USA
    J Trauma 57:961-9. 2004
    ..However, the optimal rate for the induction of hypothermic metabolic arrest following uncontrolled lethal hemorrhage (ULH) is unknown...
  10. doi request reprint Alterations in gene expression after induction of profound hypothermia for the treatment of lethal hemorrhage
    Hasan B Alam
    Division of Trauma, Department of Surgery, Massachusetts General Hospital Harvard Medical School, Boston, Massachusetts 02114, USA
    J Trauma 68:1084-98. 2010
    ..However, the precise mechanisms have not been well defined. The aim of this high-throughput study was to investigate the impact of profound hypothermia on gene expression profiles...
  11. doi request reprint Surviving lethal septic shock without fluid resuscitation in a rodent model
    Yongqing Li
    Department of Surgery, Division of Trauma, Emergency Surgery and Surgical Critical Care, Massachusetts General Hospital, Boston, MA 02114, USA
    Surgery 148:246-54. 2010
    ..The purpose of the present study was to determine whether SAHA treatment would attenuate LPS-induced shock and improve survival when given postinsult in a rodent model...
  12. ncbi request reprint Histone deacetylase as therapeutic target in a rodent model of hemorrhagic shock: effect of different resuscitation strategies on lung and liver
    Tom Lin
    Department of Surgery, Washington Hospital Center, Washington, DC, USA
    Surgery 141:784-94. 2007
    ....
  13. ncbi request reprint Impact of resuscitation strategies on the acetylation status of cardiac histones in a swine model of hemorrhage
    Hasan B Alam
    Trauma Research and Readiness Institute for Surgery, Uniformed Services University of the Health Sciences, Bethesda, MD, United States
    Resuscitation 76:299-310. 2008
    ....
  14. doi request reprint Acetylation: a novel method for modulation of the immune response following trauma/hemorrhage and inflammatory second hit in animals and humans
    Elizabeth A Sailhamer
    Department of Surgery, Division of Trauma, Emergency Surgery and Surgical Critical Care, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
    Surgery 144:204-16. 2008
    ..Correction of this imbalance with histone deacetylase inhibitors (HDACI) improves survival. We aimed to identify whether this was due to modulation of the post-shock immune response...
  15. doi request reprint Surviving blood loss without blood transfusion in a swine poly-trauma model
    Hasan B Alam
    Department of Surgery, Division of Trauma, Emergency Surgery and Surgical Critical Care, Massachusetts General Hospital Harvard Medical School, Boston, MA 02114, USA
    Surgery 146:325-33. 2009
    ..This study investigated whether VPA treatment would improve survival in a clinically relevant large animal model of poly-trauma/hemorrhagic shock, and whether the protective effects are executed through the Akt survival pathway...
  16. doi request reprint Cell protective mechanism of valproic acid in lethal hemorrhagic shock
    Yongqing Li
    Department of Surgery, Division of Trauma, Emergency Surgery and Surgical Critical Care, Massachusetts General Hospital Harvard Medical School, Boston, MA 02114, USA
    Surgery 144:217-24. 2008
    ..This study investigated whether VPA treatment works through the beta-catenin survival pathways...
  17. ncbi request reprint Hepatic and pulmonary apoptosis after hemorrhagic shock in swine can be reduced through modifications of conventional Ringer's solution
    Eduardo C Ayuste
    Uniformed Services University of the Health Sciences, Bethesda, Maryland, USA
    J Trauma 60:52-63. 2006
    ..Our hypothesis was that in a clinically relevant large animal model of hemorrhage, resuscitation with racemic lactated Ringer's solution would induce cellular apoptosis, which can be attenuated by elimination of d-lactate...
  18. doi request reprint Hypoxic "second hit" in leukocytes from trauma patients: Modulation of the immune response by histone deacetylase inhibition
    Elizabeth A Sailhamer
    Department of Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
    Cytokine 49:303-11. 2010
    ..The current experiments were designed to study the effects of HDACI after hemorrhage and severe hypoxia...
  19. ncbi request reprint Induction of profound hypothermia modulates the immune/inflammatory response in a swine model of lethal hemorrhage
    Zhang Chen
    Trauma Research and Readiness Institute for Surgery, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA
    Resuscitation 66:209-16. 2005
    ..However, the impact of this approach on the immune/inflammatory response is unknown. This experiment was conducted to test the influence of profound hypothermia on markers of immune/inflammatory system...
  20. ncbi request reprint Testing of modified zeolite hemostatic dressings in a large animal model of lethal groin injury
    Naresh Ahuja
    Department of Surgery, Uniformed Services University of the Health Sciences, Bethesda, Maryland, USA
    J Trauma 61:1312-20. 2006
    ..However, the effect of these alterations on the hemostatic efficacy is unknown. We tested modified zeolites and a chitosan based dressing against controls in a swine model of battlefield injury...
  21. doi request reprint Protective effect of suberoylanilide hydroxamic acid against LPS-induced septic shock in rodents
    Yongqing Li
    Department of Surgery, Division of Trauma, Emergency Surgery and Surgical Critical Care, Massachusetts General Hospital Harvard Medical School, Boston, Massachusetts 02114, USA
    Shock 32:517-23. 2009
    ..We report for the first time that administration of SAHA (50 mg/kg) significantly attenuates a variety of inflammatory markers and improves long-term survival after a lethal LPS insult...
  22. doi request reprint Treatment with histone deacetylase inhibitor attenuates MAP kinase mediated liver injury in a lethal model of septic shock
    Robert A Finkelstein
    Department of Surgery, Division of Trauma, Emergency Surgery and Surgical Critical Care, Massachusetts General Hospital Harvard Medical School, Boston, Massachusetts 02114, USA
    J Surg Res 163:146-54. 2010
    ..The purpose of this study was to assess the effect of SAHA treatment on MAP kinases and associated inflammatory markers in murine liver after LPS-induced injury...
  23. doi request reprint Prevention of hypoxia-induced neuronal apoptosis through histone deacetylase inhibition
    Yongqing Li
    Department of Surgery, Division of Trauma, Emergency Surgery and Surgical Critical Care, Massachusetts General Hospital Harvard Medical School, Boston, Massachusetts, USA
    J Trauma 64:863-70; discussion 870-1. 2008
    ..In the present study, neurons were subjected to severe hypoxic condition in vitro to test whether VPA would prevent hypoxia-induced apoptosis, and to explore the possible mechanisms...
  24. ncbi request reprint Learning and memory is preserved after induced asanguineous hyperkalemic hypothermic arrest in a swine model of traumatic exsanguination
    Hasan B Alam
    Department of Surgery, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA
    Surgery 132:278-88. 2002
    ..We report a novel method of doing this in a swine model of uncontrolled lethal hemorrhage (ULH) that resulted in preservation of learning ability and memory...
  25. doi request reprint Pharmacologic resuscitation: cell protective mechanisms of histone deacetylase inhibition in lethal hemorrhagic shock
    Muhammad U Butt
    Department of Surgery, Division of Trauma, Emergency Surgery, and Surgical Critical Care, Massachusetts General Hospital Harvard Medical School, Boston, Massachusetts 02114, USA
    J Surg Res 156:290-6. 2009
    ..This study investigated whether VPA treatment works through the c-Jun N-terminal kinase (JNK)/Caspase-3 survival pathways...
  26. ncbi request reprint D-lactate increases pulmonary apoptosis by restricting phosphorylation of bad and eNOS in a rat model of hemorrhagic shock
    Amin Jaskille
    Department of Surgery, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814, USA
    J Trauma 57:262-69; discussion 269-70. 2004
    ..These modified solutions however alter the function of apoptotic proteins through an unknown mechanism. We postulated that DL-LR induces apoptosis by restricting the phosphorylation of key apoptotic proteins...
  27. doi request reprint Pharmacologic resuscitation decreases circulating cytokine-induced neutrophil chemoattractant-1 levels and attenuates hemorrhage-induced acute lung injury
    Eugene Y Fukudome
    Department of Surgery, Division of Trauma, Emergency Surgery, Massachusetts General Hospital Harvard Medical School, Boston, MA, USA
    Surgery 152:254-61. 2012
    ..Herein, we have investigated the effects of HS and VPA treatment on ALI and circulating cytokines that may serve as biomarkers for the development of organ injury...
  28. ncbi request reprint Does the rate of rewarming from profound hypothermic arrest influence the outcome in a swine model of lethal hemorrhage?
    Hasan B Alam
    Trauma Research and Readiness Institute for Surgery, Uniformed Services University of the Health Sciences, Bethesda, MD, USA
    J Trauma 60:134-46. 2006
    ..The optimal rate for re-warming from a state of profound hypothermia is unknown. This experiment was designed to test the impact of different warming rates on outcome in a swine model of lethal hemorrhage from complex vascular injuries...
  29. doi request reprint Identification of a novel potential biomarker in a model of hemorrhagic shock and valproic acid treatment
    Yongqing Li
    Department of Surgery, Division of Trauma, Emergency Surgery and Surgical Critical Care, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114, USA
    J Surg Res 159:474-81. 2010
    ..Treatment with valproic acid (VPA) has been shown to up-regulate various survival pathways and improve outcome. Here we determine whether these changes would result in altered serum biomarkers...
  30. pmc Identification of citrullinated histone H3 as a potential serum protein biomarker in a lethal model of lipopolysaccharide-induced shock
    Yongqing Li
    Department of Surgery, Division of Trauma, Emergency Surgery and Surgical Critical Care, Massachusetts General Hospital Harvard Medical School, Boston, MA, USA
    Surgery 150:442-51. 2011
    ..The present study was designed to confirm these findings and to test whether the pro-survival phenotype could be detected by an early alteration in serum biomarkers...
  31. ncbi request reprint Identification of expression patterns associated with hemorrhage and resuscitation: integrated approach to data analysis
    Huazhen Chen
    Department of Surgery, Uniformed Services University of the Health Sciences, Bethesda, MD, USA
    J Trauma 60:701-23; discussion 723-4. 2006
    ....
  32. doi request reprint Surviving blood loss without fluid resuscitation
    Christian Shults
    Department of Surgery, Division of Trauma, Emergency Surgery and Surgical Critical Care, Massachusetts General Hospital Harvard Medical School, Boston, Massachusetts, USA
    J Trauma 64:629-38; discussion 638-40. 2008
    ..The current experiment was done to test whether administration of HDACI after lethal hemorrhage, without fluid resuscitation, would improve outcome by creating a pro-survival phenotype...
  33. ncbi request reprint Differential effect of resuscitation on Toll-like receptors in a model of hemorrhagic shock without a septic challenge
    Huazhen Chen
    Department of Surgery, Uniformed Services University of the Health Sciences, Bethesda, MD, United States
    Resuscitation 74:526-37. 2007
    ..This study was conducted to measure the impact of different resuscitation strategies on TLR expression and downstream signaling in key organs...
  34. pmc Effect of valproic acid on acute lung injury in a rodent model of intestinal ischemia reperfusion
    Kyuseok Kim
    Department of Surgery, Division of Trauma, Emergency Surgery and Surgical Critical Care, Massachusetts General Hospital Harvard Medical School, Boston, MA, USA
    Resuscitation 83:243-8. 2012
    ..The purpose of this study was to investigate whether VPA could affect survival and development of ALI in a rat model of intestinal I/R...
  35. ncbi request reprint Profound hypothermia is superior to ultraprofound hypothermia in improving survival in a swine model of lethal injuries
    Hasan B Alam
    Trauma Research and Readiness Institute for Surgery, Uniformed Services University of the Health Sciences, Bethesda, USA
    Surgery 140:307-14. 2006
    ..This experiment was designed to compare the impact of deep (15 degrees C), profound (10 degrees C), and ultraprofound (5 degrees C) hypothermia on survival and organ functions...
  36. ncbi request reprint Effects of lactated Ringer's solutions on human leukocytes
    Elena Koustova
    Department of Surgery, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814, USA
    J Trauma 52:872-8. 2002
    ..The aim of this study was to investigate whether racemic LR solution (containing both isomers, dl-LR) differs from LR containing L-isomer only (L-LR)...
  37. pmc Histone deacetylase inhibitor suberoylanilide hydroxamic acid attenuates Toll-like receptor 4 signaling in lipopolysaccharide-stimulated mouse macrophages
    Wei Chong
    Department of Surgery, Division of Trauma, Emergency Surgery and Surgical Critical Care, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114, USA
    J Surg Res 178:851-9. 2012
    ..This study was designed to assess whether SAHA affects LPS/Toll-like receptor 4 signaling through acetylation of heat shock protein 90 (HSP90) and degradation of its client protein interleukin-1 receptor-associated kinase 1 (IRAK1)...
  38. pmc Histone deacetylase inhibitor treatment attenuates MAP kinase pathway activation and pulmonary inflammation following hemorrhagic shock in a rodent model
    Ashley R Kochanek
    Department of Surgery, Division of Trauma, Emergency Surgery and Surgical Critical Care, Massachusetts General Hospital Harvard Medical School, Boston, Massachusetts 02114, USA
    J Surg Res 176:185-94. 2012
    ..The aim of the current study was to examine whether these protective effects were due to attenuation of mitogen activated protein kinase (MAPK) signaling pathways, which are known to promote inflammation and apoptosis...
  39. doi request reprint Development and testing of low-volume hyperoncotic, hyperosmotic spray-dried plasma for the treatment of trauma-associated coagulopathy
    Fahad Shuja
    Department of Surgery, Massachusetts General Hospital Harvard Medical School, Boston, Massachusetts 02114, USA
    J Trauma 70:664-71. 2011
    ..To enhance the logistical advantage (lower volume and weight), we developed and tested a hyperoncotic, hyperosmotic spray-dried plasma (SDP) product in a multiple injuries/hemorrhagic shock swine model...
  40. ncbi request reprint Hepatic apoptosis after hemorrhagic shock in rats can be reduced through modifications of conventional Ringer's solution
    Amin Jaskille
    Department of Surgery, Uniformed Services University of the Health Sciences, Bethesda, MD, USA
    J Am Coll Surg 202:25-35. 2006
    ..This study was conducted to determine if the elimination of D-lactate isomer would attenuate apoptosis in the liver, and to investigate the possible mechanisms...
  41. doi request reprint Synergistic effects of hypertonic saline and valproic acid in a lethal rat two-hit model
    Zhengcai Liu
    Division of Trauma, Emergency Surgery and Surgical Critical Care, Department of Surgery, Massachusetts General Hospital Harvard Medical School, Boston, MA, USA
    J Trauma Acute Care Surg 74:991-7; discussion 997-8. 2013
    ..In the present study, we examined whether combination of HTS with VPA would allow us to achieve survival advantage at a lower dose of VPA (200 mg/kg)...
  42. pmc Creating a "pro-survival" phenotype through epigenetic modulation
    Yongqing Li
    Department of Surgery, Division of Trauma, Emergency Surgery and Surgical Critical Care, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
    Surgery 152:455-64. 2012
    ..The precise mechanisms, however, have not been well-defined. The aim of this study was to investigate the impact of SAHA treatment on gene expression profiles at an early stage of shock...
  43. doi request reprint Traumatic brain injury and hemorrhagic shock: evaluation of different resuscitation strategies in a large animal model of combined insults
    Guang Jin
    Division of Trauma, Emergency Surgery and Surgical Critical Care, Department of Surgery, Massachusetts General Hospital Harvard Medical School, Boston, MA 02114, USA
    Shock 38:49-56. 2012
    ..Early administration of FFP reduces the size of brain lesion and associated swelling in a large animal model of TBI + HS. In contrast, artificial colloid (Hex) decreases swelling without reducing the actual size of the brain lesion...
  44. ncbi request reprint Valproic acid prevents hemorrhage-associated lethality and affects the acetylation pattern of cardiac histones
    Earl Gonzales
    Department of Surgery, Trauma Research and Readiness Institute for Surgery, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA
    Shock 25:395-401. 2006
    ..Our results reveal that VPA offers considerable protection in the hemorrhagic shock model and suggest a role for HDAC inhibition in mediating VPA actions...
  45. pmc Pharmacologic resuscitation promotes survival and attenuates hemorrhage-induced activation of extracellular signal-regulated kinase 1/2
    Eugene Y Fukudome
    Department of Surgery, Division of Trauma, Emergency Surgery, and Surgical Critical Care, Massachusetts General Hospital Harvard Medical School, Boston, Massachusetts, USA
    J Surg Res 163:118-26. 2010
    ..Here we have investigated the effects of VPA treatment on extracellular signal-regulated kinase 1/2 (ERK) activation, as ERK is well known to modulate cell death, gene expression, and inflammation...
  46. doi request reprint Histone deacetylase inhibitors prevent apoptosis following lethal hemorrhagic shock in rodent kidney cells
    Nikolaos Zacharias
    Department of Surgery, Division of Trauma, Emergency Surgery and Surgical Critical Care, Massachusetts General Hospital Harvard Medical School, Boston, MA 02114, USA
    Resuscitation 82:105-9. 2011
    ..These agents are known to acetylate insulin receptor substrate-I (IRS-I), which in turn activates the Akt survival pathway. This study investigated whether HDACI exert their beneficial effects through the Akt survival pathway...
  47. ncbi request reprint Ketone and pyruvate Ringer's solutions decrease pulmonary apoptosis in a rat model of severe hemorrhagic shock and resuscitation
    Elena Koustova
    Department of Surgery, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA
    Surgery 134:267-74. 2003
    ..We tested whether this effect was related to BHB-induced metabolic modulations...
  48. ncbi request reprint Human polymorphonuclear cell death after exposure to resuscitation fluids in vitro: apoptosis versus necrosis
    Kathleen Stanton
    Department of Surgery, Uniformed Services University of Health Sciences, Bethesda, Maryland 20814, USA
    J Trauma 54:1065-74; discussion 1075-6. 2003
    ..To test this, we studied cellular death (apoptosis and necrosis) in human PMNs after brief exposure to different hypertonic and isotonic fluids...
  49. ncbi request reprint Abdominal insufflation for control of bleeding after severe splenic injury
    George C Velmahos
    Division of Trauma, Emergency Surgery, and Surgical Critical Care, Department of Surgery, The Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA
    J Trauma 63:285-8; discussion 288-90. 2007
    ..This model represents a clinically relevant scenario of continuous bleeding, which does not kill the patient immediately but may ultimately result in death if not managed early...
  50. doi request reprint Development of a lethal, closed-abdomen grade V hepato-portal injury model in non-coagulopathic swine
    Michael J Duggan
    Department of Surgery, Division of Trauma, Emergency Surgery and Surgical Critical Care, Massachusetts General Hospital Harvard Medical School, Boston, Massachusetts
    J Surg Res 182:101-7. 2013
    ..Despite this need, there is no existing closed-cavity animal model to assess new hemostatic agents for the preoperative control of intra-abdominal hemorrhage...
  51. ncbi request reprint Cardiac histones are substrates of histone deacetylase activity in hemorrhagic shock and resuscitation
    Tom Lin
    Department of Surgery, Uniformed Services University of the Health Sciences, Bethesda, MD, USA
    Surgery 139:365-76. 2006
    ..The goals of this study were to determine whether hemorrhage results in deacetylation of cardiac histones and whether this can be corrected through the application of different resuscitation strategies or specific HDAC inhibitors...
  52. ncbi request reprint New developments in fluid resuscitation
    Hasan B Alam
    Department of Surgery, Massachusetts General Hospital, 55 Fruit Street, WHT 1, Boston, MA 02114, USA
    Surg Clin North Am 87:55-72, vi. 2007
    ..This article summarizes a number of studies that have evaluated the cellular toxicities of commonly used resuscitation fluids, to highlight the need for the development of new fluids...
  53. ncbi request reprint Differential expression of extracellular matrix remodeling genes in rat model of hemorrhagic shock and resuscitation
    Huazhen Chen
    Department of Surgery, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA
    J Surg Res 123:235-44. 2005
    ..However, the effect of different resuscitation protocols at the level of protein expression and function remains unknown...
  54. ncbi request reprint Administration of recombinant interleukin-11 improves the hemodynamic functions and decreases third space fluid loss in a porcine model of hemorrhagic shock and resuscitation
    Kaneatsu Honma
    Department of Medicine, Uniformed Services University of the Health Sciences, F Edward Hebert School of Medicine, Bethesda, Maryland 20814 4799, USA
    Shock 23:539-42. 2005
    ..This strategy also significantly reduced the extent of third space fluid losses while also having a favorable impact on the intravascular volume status as evidenced by the improved urine output...
  55. ncbi request reprint Development and testing of portable pump for the induction of profound hypothermia in a Swine model of lethal vascular injuries
    Hasan B Alam
    Department of Surgery, Uniformed Services University of the Health Sciences, Bethesda, MD, USA
    J Trauma 61:1321-9. 2006
    ..In this experiment, a portable prototype pump was tested and its performance was compared with the regular roller pump in a swine model of lethal vascular injuries...
  56. pmc Anti-inflammatory properties of histone deacetylase inhibitors: a mechanistic study
    Wei Chong
    Division of Trauma, Emergency Surgery and Surgical Critical Care, Department of Surgery, Massachusetts General Hospital, 165 Cambridge St, Boston, MA 02114, USA
    J Trauma Acute Care Surg 72:347-53; discussion 353-4. 2012
    ....
  57. ncbi request reprint Energy substrate-supplemented resuscitation affects brain monocarboxylate transporter levels and gliosis in a rat model of hemorrhagic shock
    Tom Lin
    Department of Surgery, Uniformed Services University of the Health Sciences, Bethesda, Maryland, USA
    J Trauma 59:1191-202; discussion 1202. 2005
    ..The hypothesis was that resuscitation with MC-based fluids would be accompanied by MCT1 up-regulation and glial response...
  58. doi request reprint Fluid resuscitation: past, present, and the future
    Heena P Santry
    Department of Surgery, Division of Trauma, Emergency Surgery, and Surgical Critical Care, Massachusetts General Hospital Harvard Medical School, Boston, MA 02114, USA
    Shock 33:229-41. 2010
    ..Thus, we describe how some novel resuscitation strategies aimed at preventing or ameliorating cellular injury may become clinically available in the future...
  59. ncbi request reprint A portable handpump is effective in the evacuation of hemothorax in a swine model of penetrating chest injury
    Amin Jaskille
    Department of Surgery, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, MD 20814, USA
    J Trauma 55:864-8. 2003
    ..A swine model of penetrating chest injury was designed to test this concept. Our hypothesis was that an HP would be as effective as the standard of care for the evacuation of a large hemopneumothorax...
  60. ncbi request reprint cDNA profiling in leukocytes exposed to hypertonic resuscitation fluids
    Vadim Gushchin
    Department of Surgery, Washington Hospital Center, Washington, DC, USA
    J Am Coll Surg 197:426-32. 2003
    ..In hypertonic saline Dextran (HTD), the hypertonic component determines the leukocyte functional behavior and subsequently activation response. We investigated whether leukocyte gene expression is analogously affected...
  61. ncbi request reprint Searching for the optimal resuscitation method: recommendations for the initial fluid resuscitation of combat casualties
    Peter Rhee
    Trauma Research and Readiness Institute for Surgery, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA
    J Trauma 54:S52-62. 2003
    ..Finally, because of the uniqueness of combat care, a set of new recommendations for initial fluid resuscitation of combat casualties is proposed...
  62. doi request reprint Fulminant Clostridium difficile colitis: patterns of care and predictors of mortality
    Elizabeth A Sailhamer
    Department of Surgery, Division of Trauma, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA
    Arch Surg 144:433-9; discussion 439-40. 2009
    ..There exist predictors of mortality and the need for colectomy among patients with fulminant Clostridium difficile colitis...
  63. pmc Hypothermia in bleeding trauma: a friend or a foe?
    Tareq Kheirbek
    Department of Surgery, Division of Trauma, Emergency Surgery and Surgical Critical Care, Massachusetts General Hospital Harvard Medical School, Boston, MA, USA
    Scand J Trauma Resusc Emerg Med 17:65. 2009
    ..While many of the advantageous molecular and physiological effects of induced hypothermia have been outlined in animal models, rigorous clinical investigations are needed to translate these promising findings into clinical practice...
  64. ncbi request reprint Making sense of the preclinical literature on advanced hemostatic products
    Anthony E Pusateri
    US Army Institute of Surgical Research, Fort Sam Houston, Texas 78234, and the Department of Surgery, Uniformed Services University of the Health Sciences, Bethesda, Maryland, USA
    J Trauma 60:674-82. 2006
    ..Mission and training requirements will dictate the use of these products by military and civilian prehospital care providers...
  65. ncbi request reprint Cytokine expression profiling in human leukocytes after exposure to hypertonic and isotonic fluids
    Vadim Gushchin
    Department of Surgery, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814, USA
    J Trauma 52:867-71. 2002
    ..The cDNA array method was used to profile transcriptional responses after exposure to hypertonic and isotonic fluids...
  66. ncbi request reprint Is upper extremity deep venous thrombosis underdiagnosed in trauma patients?
    Konstantinos Spaniolas
    Division of Trauma, Emergency Surgery and Surgical Critical Care, Department of Surgery, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114, USA
    Am Surg 74:124-8. 2008
    ..UEDVT is an uncommon event with unclear significance in trauma. Aggressive screening did not result in a higher rate of UEDVT diagnosis, nor an opportunity to prevent PE...
  67. pmc Modulation of acetylation: creating a pro-survival and anti-inflammatory phenotype in lethal hemorrhagic and septic shock
    Yongqing Li
    Department of Surgery, Division of Trauma, Emergency Surgery and Surgical Critical Care, Massachusetts General Hospital Harvard Medical School, 165 Cambridge Street, Suite 810, Boston, MA 02114, USA
    J Biomed Biotechnol 2011:523481. 2011
    ..This paper highlights the emerging roles of HDACIs as acetylation modulators in models of hemorrhagic and septic shock and explains some contradictions encountered in previous studies...
  68. doi request reprint Hypothermia in multisystem trauma
    Eugene Y Fukudome
    Division of Trauma, Emergency Surgery and Surgical Critical Care, Department of Surgery, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA
    Crit Care Med 37:S265-72. 2009
    ..Hypothermia may be an effective therapeutic approach for otherwise lethal traumatic hemorrhage, and a clinical trial to determine its utility is warranted...
  69. doi request reprint Creating a pro-survival and anti-inflammatory phenotype by modulation of acetylation in models of hemorrhagic and septic shock
    Yongqing Li
    Department of Surgery, Division of Trauma, Emergency Surgery and Surgical Critical Care, Massachusetts General Hospital Harvard Medical School, Boston, MA, USA
    Adv Exp Med Biol 710:107-33. 2012
    ....
  70. doi request reprint Diazoxide increases liver and kidney HSP25 and HSP70 after shock and stroke
    Joseph C O'Sullivan
    Graduate Program in Neuroscience, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814 4799, USA
    J Surg Res 149:120-30. 2008
    ..Our team tested the hypothesis that pharmacological induction of ischemic preconditioning with DZ can offer cytoprotection and preserve vital tissues after hemorrhagic shock and stroke...
  71. ncbi request reprint Alterations of cullin-5 mRNA levels in the rat central nervous system following hemorrhagic shock
    Thomas E Ceremuga
    Graduate Program in Neuroscience, Department of Surgery, F Edmund H├ębert School of Medicine, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, MD 20814, USA
    Neurol Res 25:211-6. 2003
    ..This is the first report of cul-5 mRNA regulation by hemorrhagic shock. Evidence indicates this protein may have a regulatory role in ubiquitin-proteasomal protein degradation in response to hemorrhagic shock...
  72. doi request reprint Long-term outcome of acellular dermal matrix when used for large traumatic open abdomen
    Marc A de Moya
    Division of Trauma, Emergency Surgery, and Surgical Critical Care, Department of Surgery, Massachusetts General Hospital, Boston, Massachusetts, USA
    J Trauma 65:349-53. 2008
    ..Previous studies have not examined the long-term outcomes in trauma patients with abdominal wall reconstructions...
  73. doi request reprint Putting life on hold-for how long? Profound hypothermic cardiopulmonary bypass in a Swine model of complex vascular injuries
    Hasan B Alam
    Division of Trauma, Emergency Surgery and Surgical Critical Care, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA
    J Trauma 64:912-22. 2008
    ..5 degrees C/min) for best results. However, the maximum duration of hypothermic arrest in a clinically relevant trauma model remains unknown...
  74. ncbi request reprint Host-guest composites for induced hemostasis and therapeutic healing in traumatic injuries
    Todd A Ostomel
    Department of Chemistry and Biochemistry, University of California Santa Barbara, Santa Barbara, California, 93106, USA
    J Thromb Thrombolysis 22:55-67. 2006
    ..Five ion exchanged derivatives of the parent HA were prepared and characterized with regard to their material and thermal properties, in vitro hemostatic efficacy, and antibacterial activity...
  75. ncbi request reprint Profound hypothermic cardiopulmonary bypass facilitates survival without a high complication rate in a swine model of complex vascular, splenic, and colon injuries
    Elizabeth A Sailhamer
    Division of Trauma, Emergency Surgery and Surgical Critical Care, Massachusetts General Hospital Harvard Medical School, Boston, MA 02114, USA
    J Am Coll Surg 204:642-53. 2007
    ....
  76. ncbi request reprint Abdominal insufflation decreases blood loss without worsening the inflammatory response: implications for prehospital control of internal bleeding
    George C Velmahos
    Division of Trauma, Emergency Surgery, and Surgical Critical Care, The Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA
    Am Surg 74:297-301. 2008
    ..Our conclusion is that AI reduces blood loss from splenic injury without a measurable effect on the early inflammatory response in a clinically relevant animal model...
  77. ncbi request reprint Dose-dependent effects of recombinant human interleukin-11 on the systemic hemodynamic function and urination
    Kaneatsu Honma
    Division of Operative Management, Koga Hospital 21, Kurume, Japan
    Kurume Med J 54:73-6. 2007
    ..05). In conclusion, even a low dose of rhIL-11 improved the hemodynamic functions dose-dependently in a porcine model of hemorrhagic shock, although the relationship did not demonstrate a simple linearity...
  78. doi request reprint Just do it
    Hasan B Alam
    Crit Care Med 36:2456-7. 2008
  79. ncbi request reprint Routine repeat head CT for minimal head injury is unnecessary
    George C Velmahos
    Division of Trauma, Emergency Surgery, and Surgical Critical Care, Department of Neurosurgery, and Biostatistics Center DJD, Massachusetts General Hospital, Harvard School of Medicine, Boston, MA 02114, USA
    J Trauma 60:494-9; discussion 499-501. 2006
    ..RRHCT is ordered based on the premise that significant injury progression may take place in the absence of clinical deterioration...
  80. doi request reprint Bedside placement of removable vena cava filters guided by intravascular ultrasound in the critically injured
    Konstantinos Spaniolas
    Division of Trauma, Emergency Surgery, and Surgical Critical Care, Massachusetts General Hospital and Harvard Medical School, 165 Cambridge Street, Suite 810, Boston, MA 02114, USA
    World J Surg 32:1438-43. 2008
    ..Intravascular ultrasound (IVUS) eliminates such problems. The objective of the present study was to analyze the safety and feasibility of IVUS-guided bedside RVCF placement in critically injured patients...