G Widmer

Summary

Affiliation: Tufts University
Country: USA

Publications

  1. pmc Detection and genotyping of oocysts of Cryptosporidium parvum by real-time PCR and melting curve analysis
    Sultan Tanriverdi
    Department of Pediatric Hematology Oncology, School of Medicine, Cukurova University, 01330 Adana, Turkey
    J Clin Microbiol 40:3237-44. 2002
  2. ncbi request reprint Identification of genotypically mixed Cryptosporidium parvum populations in humans and calves
    Sultan Tanriverdi
    Faculty of Medicine, Mustafa Kemal University, Antakya, Turkey
    Mol Biochem Parasitol 130:13-22. 2003
  3. ncbi request reprint Differential evolution of repetitive sequences in Cryptosporidium parvum and Cryptosporidium hominis
    Sultan Tanriverdi
    Tufts University School of Veterinary Medicine, Division of Infectious Diseases, 200 Westborough Road, North Grafton, MA 01536, USA
    Infect Genet Evol 6:113-22. 2006
  4. pmc Emergence of distinct genotypes of Cryptosporidium parvum in structured host populations
    Sultan Tanriverdi
    Division of Infectious Diseases, Tufts Cummings School of Veterinary Medicine, 200 Westboro Road, North Grafton, MA 01536, USA
    Appl Environ Microbiol 72:2507-13. 2006
  5. ncbi request reprint Genetic crosses in the apicomplexan parasite Cryptosporidium parvum define recombination parameters
    Sultan Tanriverdi
    Tufts Cummings School of Veterinary Medicine, Division of Infectious Diseases, North Grafton, MA, USA
    Mol Microbiol 63:1432-9. 2007
  6. pmc Class B alkaline stabilization to achieve pathogen inactivation
    Christine L Bean
    Department of Microbiology, University of New Hampshire, 35 Colovos Rd, ETB Hall Rm 230, Durham, NH 03824, USA
    Int J Environ Res Public Health 4:53-60. 2007
  7. pmc Comparative genome analysis of two Cryptosporidium parvum isolates with different host range
    Giovanni Widmer
    Tufts Cummings School of Veterinary Medicine, Division of Infectious Diseases, North Grafton, MA 01536, USA
    Infect Genet Evol 12:1213-21. 2012
  8. pmc Genomics and population biology of Cryptosporidium species
    G Widmer
    Division of Infectious Diseases, Tufts Cummins School of Veterinary Medicine, North Grafton, MA 01536, USA
    Parasite Immunol 34:61-71. 2012
  9. pmc A comparison of the Giardia lamblia trophozoite and cyst transcriptome using microarrays
    Zahra Faghiri
    Division of Infectious Diseases, Tufts Cummings School of Veterinary Medicine, 200 Westboro Road, North Grafton, MA, USA
    BMC Microbiol 11:91. 2011
  10. pmc Host-specific segregation of ribosomal nucleotide sequence diversity in the microsporidian Enterocytozoon bieneusi
    Giovanni Widmer
    Tufts Cummings School of Veterinary Medicine, Division of Infectious Diseases, Tufts University, Bldg 20, 200 Westboro Rd, North Grafton, MA 01536, United States
    Infect Genet Evol 10:122-8. 2010

Research Grants

  1. Genetics of Cryptosporidium parvum
    Giovanni Widmer; Fiscal Year: 2002
  2. Genetics of Cryptosporidium parvum
    Giovanni Widmer; Fiscal Year: 2009
  3. Functional Genomics of Cryptosporidium parvum
    Giovanni Widmer; Fiscal Year: 2007
  4. Genetics of Cryptosporidium parvum
    Giovanni Widmer; Fiscal Year: 2006
  5. Functional Genemics of Cryptosporidium parvum
    Giovanni Widmer; Fiscal Year: 2006
  6. Functional Genemics of Cryptosporidium parvum
    Giovanni Widmer; Fiscal Year: 2005
  7. Genetics of Cryptosporidium parvum
    Giovanni Widmer; Fiscal Year: 2005
  8. Genetic Transformation of Cryptosporidium parvum
    Giovanni Widmer; Fiscal Year: 2005
  9. Functional Genomics of Cryptosporidium parvum
    Giovanni Widmer; Fiscal Year: 2004
  10. Genetics of Cryptosporidium parvum
    Giovanni Widmer; Fiscal Year: 2004

Collaborators

Detail Information

Publications46

  1. pmc Detection and genotyping of oocysts of Cryptosporidium parvum by real-time PCR and melting curve analysis
    Sultan Tanriverdi
    Department of Pediatric Hematology Oncology, School of Medicine, Cukurova University, 01330 Adana, Turkey
    J Clin Microbiol 40:3237-44. 2002
    ..Both methods discriminated between type 1 and type 2 C. parvum on the basis of melting curve analysis. To our knowledge, this is the first report describing the application of melting curve analysis for genotyping of C. parvum oocysts...
  2. ncbi request reprint Identification of genotypically mixed Cryptosporidium parvum populations in humans and calves
    Sultan Tanriverdi
    Faculty of Medicine, Mustafa Kemal University, Antakya, Turkey
    Mol Biochem Parasitol 130:13-22. 2003
    ..parvum type 1...
  3. ncbi request reprint Differential evolution of repetitive sequences in Cryptosporidium parvum and Cryptosporidium hominis
    Sultan Tanriverdi
    Tufts University School of Veterinary Medicine, Division of Infectious Diseases, 200 Westborough Road, North Grafton, MA 01536, USA
    Infect Genet Evol 6:113-22. 2006
    ..parvum and C. hominis repeats did not overlap. Assuming that C. parvum and C. hominis evolved from a common ancestor, these observations suggest a differential evolution of repeat length at these loci...
  4. pmc Emergence of distinct genotypes of Cryptosporidium parvum in structured host populations
    Sultan Tanriverdi
    Division of Infectious Diseases, Tufts Cummings School of Veterinary Medicine, 200 Westboro Road, North Grafton, MA 01536, USA
    Appl Environ Microbiol 72:2507-13. 2006
    ..This may explain the frequent detection of host-specific genotypes with unknown taxonomic status in surface water and the existence of geographically restricted C. hominis genotypes in humans...
  5. ncbi request reprint Genetic crosses in the apicomplexan parasite Cryptosporidium parvum define recombination parameters
    Sultan Tanriverdi
    Tufts Cummings School of Veterinary Medicine, Division of Infectious Diseases, North Grafton, MA, USA
    Mol Microbiol 63:1432-9. 2007
    ..C. parvum exhibits a high rate of recombination commensurate with other Apicomplexa...
  6. pmc Class B alkaline stabilization to achieve pathogen inactivation
    Christine L Bean
    Department of Microbiology, University of New Hampshire, 35 Colovos Rd, ETB Hall Rm 230, Durham, NH 03824, USA
    Int J Environ Res Public Health 4:53-60. 2007
    ....
  7. pmc Comparative genome analysis of two Cryptosporidium parvum isolates with different host range
    Giovanni Widmer
    Tufts Cummings School of Veterinary Medicine, Division of Infectious Diseases, North Grafton, MA 01536, USA
    Infect Genet Evol 12:1213-21. 2012
    ..hominis than to the zoonotic C. parvum reference. Because C. hominis and anthroponotic C. parvum share a similar host range, this unexpected observation suggests that proteins encoded by these genes may influence the host range...
  8. pmc Genomics and population biology of Cryptosporidium species
    G Widmer
    Division of Infectious Diseases, Tufts Cummins School of Veterinary Medicine, North Grafton, MA 01536, USA
    Parasite Immunol 34:61-71. 2012
    ..Genotyping of oocysts excreted by human and animal hosts using such markers has revealed many new species and genotypes, and is leading to a better understanding of the epidemiology of cryptosporidiosis...
  9. pmc A comparison of the Giardia lamblia trophozoite and cyst transcriptome using microarrays
    Zahra Faghiri
    Division of Infectious Diseases, Tufts Cummings School of Veterinary Medicine, 200 Westboro Road, North Grafton, MA, USA
    BMC Microbiol 11:91. 2011
    ....
  10. pmc Host-specific segregation of ribosomal nucleotide sequence diversity in the microsporidian Enterocytozoon bieneusi
    Giovanni Widmer
    Tufts Cummings School of Veterinary Medicine, Division of Infectious Diseases, Tufts University, Bldg 20, 200 Westboro Rd, North Grafton, MA 01536, United States
    Infect Genet Evol 10:122-8. 2010
    ..Due to the possibility of genetic recombination in this species, additional unlinked genetic markers need to be developed and included in future studies...
  11. pmc Meta-analysis of a polymorphic surface glycoprotein of the parasitic protozoa Cryptosporidium parvum and Cryptosporidium hominis
    G Widmer
    Tufts Cummings School of Veterinary Medicine, Division of Infectious Diseases, North Grafton, MA, USA
    Epidemiol Infect 137:1800-8. 2009
    ..With the notable exception of a group of alleles restricted to humans, C. parvum alleles are found in ruminants and humans...
  12. ncbi request reprint Population genetics of Cryptosporidium parvum
    Giovanni Widmer
    Tufts University School of Veterinary Medicine, 200 Westboro Road, North Grafton, MA 01536, USA
    Trends Parasitol 20:3-6; discussion 6. 2004
  13. ncbi request reprint Preferential infection of dividing cells by Cryptosporidium parvum
    G Widmer
    Tufts Cummings School of Veterinary Medicine, Division of Infectious Diseases, 200 Westboro Road, North Grafton, MA 01536, USA
    Parasitology 133:131-8. 2006
    ..Consistent with previous observations showing that C. parvum induces apoptosis in cell monolayers, infection was accompanied by a significant increase in the proportion of mitotic cells...
  14. ncbi request reprint Adaptation of Cryptosporidium oocysts to different excystation conditions
    G Widmer
    Tufts Cummings School of Veterinary Medicine, Division of Infectious Diseases, 200 Westboro Road, North Grafton, MA 01536, USA
    Parasitology 134:1583-8. 2007
    ....
  15. ncbi request reprint Structural and biochemical alterations in Giardia lamblia cysts exposed to ozone
    Giovanni Widmer
    Tufts University School of Veterinary Medicine, Building 20, 200 Westboro Road, North Grafton, Massachusetts 01536, USA
    J Parasitol 88:1100-6. 2002
    ..Exposure for 60 sec or longer resulted in extensive protein degradation and in the disappearance of a cyst wall and a trophozoite antigen...
  16. pmc Comparison of single- and multilocus genetic diversity in the protozoan parasites Cryptosporidium parvum and C. hominis
    Giovanni Widmer
    Tufts Cummings School of Veterinary Medicine, Division of Infectious Diseases, North Grafton, MA 01536, USA
    Appl Environ Microbiol 76:6639-44. 2010
    ..parvum and C. hominis populations. Unless parasite virulence is controlled at the GP60 locus, attempts to find associations within species or subspecies between GP60 and phenotype are unlikely to be successful...
  17. ncbi request reprint Genomics and genetics of Cryptosporidium parvum: the key to understanding cryptosporidiosis
    Giovanni Widmer
    Tufts University School of Veterinary Medicine, Division of Infectious Diseases, 200 Westboro Road, North Grafton, MA 01536, USA
    Microbes Infect 4:1081-90. 2002
    ..The approach to and the relevance of sequencing the genomes of C. parvum type 1 and type 2 are discussed, as well as new insights into the genetic heterogeneity of this species...
  18. pmc Development and application of genetic probes for detection of Enterocytozoon bieneusi in formalin-fixed stools and in intestinal biopsy specimens from infected patients
    A Carville
    Division of Infectious Diseases, Tufts University School of Veterinary Medicine, North Grafton, Massachusetts 01536, USA
    Clin Diagn Lab Immunol 4:405-8. 1997
    ..Both the PCR and in situ hybridization procedures are sensitive diagnostic tools which will complement currently available techniques and enable the differentiation of E. bieneusi from other microsporidia to be made...
  19. pmc Sequence polymorphism in the beta-tubulin gene reveals heterogeneous and variable population structures in Cryptosporidium parvum
    G Widmer
    Division of Infectious Diseases, Tufts University School of Veterinary Medicine, North Grafton, Massachusetts 01536, USA
    Appl Environ Microbiol 64:4477-81. 1998
    ..A comparison of multiple samples of a calf-propagated laboratory isolate showed that the ratio of different beta-tubulin alleles fluctuated during serial passage...
  20. pmc Transmission and serial propagation of Enterocytozoon bieneusi from humans and Rhesus macaques in gnotobiotic piglets
    I Kondova
    Division of Infectious Disease, Tufts University School of Veterinary Medicine, North Grafton, Massachusetts 01536, USA
    Infect Immun 66:5515-9. 1998
    ..E. bieneusi infection induced no symptoms. The development of an animal model for E. bieneusi will open up new opportunities for investigating this parasite...
  21. ncbi request reprint A study of Cryptosporidium parvum genotypes and population structure
    G Widmer
    Tufts University School of Veterinary Medicine, Division of Infectious Diseases, North Grafton, Massachusetts, USA
    Mem Inst Oswaldo Cruz 93:685-6. 1998
    ..Sequence analysis of the beta-tubulin intron revealed additional polymorphism. The stability of the genetic profiles following passage of C. parvum isolates between different hosts was investigated...
  22. pmc beta-tubulin mRNA as a marker of Cryptosporidium parvum oocyst viability
    G Widmer
    Division of Infectious Diseases, Tufts University School of Veterinary Medicine, North Grafton, Massachusetts 01536, USA
    Appl Environ Microbiol 65:1584-8. 1999
    ..parvum oocysts. The methodological similarity between PCR detection and RT-PCR viability analysis could facilitate the development of a combined detection and viability assay...
  23. pmc Genetic analysis of a Cryptosporidium parvum human genotype 1 isolate passaged through different host species
    D E Akiyoshi
    Division of Infectious Diseases, Tufts University School of Veterinary Medicine, North Grafton, Massachusetts 01536, USA
    Infect Immun 70:5670-5. 2002
    ..The genotypic and phenotypic characterization of TU502 is significant since it is the isolate selected to sequence the genome of C. parvum genotype 1 and is currently used in several research projects including human volunteer studies...
  24. pmc Cryptosporidium parvum DNA replication in cell-free culture
    L Zhang
    Division of Infectious Diseases, Tufts Cummings School of Veterinary Medicine, North Grafton, Massachusetts 01536, USA
    J Parasitol 95:1239-42. 2009
    ..These observations are indicative of a modest proliferation of C. parvum in cell-free culture...
  25. pmc Paromomycin and geneticin inhibit intracellular Cryptosporidium parvum without trafficking through the host cell cytoplasm: implications for drug delivery
    J K Griffiths
    Division of Infectious Diseases, Department of Biomedical Sciences, Tufts University School of Veterinary Medicine, Grafton, Massachusetts 01536, USA
    Infect Immun 66:3874-83. 1998
    ..parvum...
  26. pmc A hundred-year retrospective on cryptosporidiosis
    Saul Tzipori
    Tufts Cummings School of Veterinary Medicine, Division of Infectious Diseases, North Grafton, MA 01536, USA
    Trends Parasitol 24:184-9. 2008
    ....
  27. ncbi request reprint Characterization of subtilase protease in Cryptosporidium parvum and C. hominis
    Xiaochuan Feng
    Division of Infectious Diseases, Tufts Cummings School of Veterinary Medicine, North Grafton, Massachusetts 01536, USA
    J Parasitol 93:619-26. 2007
    ....
  28. ncbi request reprint Molecular characterization of ribonucleotide reductase from Cryptosporidium parvum
    Donna E Akiyoshi
    Division of Infectious Diseases, Tufts University School of Veterinary Medicine, North Grafton, MA 01536, USA
    DNA Seq 13:167-72. 2002
    ..parvum genotype 2 isolate, GCH1. Southern blot analysis showed that these subunits are encoded by single copy genes. Both subunits have been expressed as recombinant proteins in Escherichia coli...
  29. pmc Inferences about the global population structures of Cryptosporidium parvum and Cryptosporidium hominis
    Sultan Tanriverdi
    Tufts Cummings School of Veterinary Medicine, Division of Infectious Diseases, North Grafton, MA 01536, USA
    Appl Environ Microbiol 74:7227-34. 2008
    ..The relative contribution of each pattern appears to vary between the regions, perhaps dependent on the prevailing ecological determinants of transmission...
  30. ncbi request reprint Experimental evidence for genetic recombination in the opportunistic pathogen Cryptosporidium parvum
    Xiaochuan Feng
    Division of Infectious Diseases, Tufts University School of Veterinary Medicine, 200 Westboro Road, Building 20, North Grafton, MA 01536, USA
    Mol Biochem Parasitol 119:55-62. 2002
    ..parvum subtypes and host-specific genotypes...
  31. ncbi request reprint Quantitative tracking of Cryptosporidium infection in cell culture with CFSE
    Hanping Feng
    Division of Infectious Diseases, Tufts University, Cummings School of Veterinary Medicine, North Grafton, Massachusetts 01536, USA
    J Parasitol 92:1350-4. 2006
    ..Unlike immunofluorescent assays, CFSE labeling permitted the tracking of the initial invasion of C. parvum. Such an assay may be useful for studying the dynamics of host cell-parasite interaction and possibly for drug screening...
  32. pmc Serial propagation of the microsporidian Enterocytozoon bieneusi of human origin in immunocompromised rodents
    Xiaochuan Feng
    Division of Infectious Diseases, Tufts University Cummings School of Veterinary Medicine, 200 Westborough Road, North Grafton, Massachusetts 01536, USA
    Infect Immun 74:4424-9. 2006
    ..Rodent models and a nonhuman source of fresh spores will considerably enhance future investigations on this important opportunistic pathogen, including the screening and evaluation of urgently needed chemotherapeutic agents...
  33. ncbi request reprint Infectivity of a Cryptosporidium parvum isolate of cervine origin for healthy adults and interferon-gamma knockout mice
    Pablo C Okhuysen
    Department of Medicine, Division of Infectious Diseases, and School of Public Health, University of Texas Houston Health Science Center, Houston, TX 77030, USA
    J Infect Dis 185:1320-5. 2002
    ..The Moredun isolate was more pathogenic than the reference GCH-1 isolate. The GKO mouse model of cryptosporidiosis is useful for discerning isolate-specific differences in pathogenicity...
  34. doi request reprint High-throughput screening in suboptimal growth conditions identifies agonists of Giardia lamblia proliferation
    Z Faghiri
    Tufts Cummings School of Veterinary Medicine, 200 Westboro Road, North Grafton, Massachusetts 01536, USA
    Parasitology 138:194-200. 2011
    ..These observations illustrate the power of chemical genetics for identifying pathways controlling specific traits in G. lamblia...
  35. ncbi request reprint Genotyping of Cryptosporidium parvum with microsatellite markers
    Giovanni Widmer
    Tufts University School of Veterinary Medicine, North Grafton, MA, USA
    Methods Mol Biol 268:177-87. 2004
    ..parvum, we have pursued two methods, a traditional polymerase chain reaction (PCR) method followed by gel electrophoresis, and real-time PCR using SYBR Green I melting curve analysis for allele identification...
  36. pmc Identification of growth inhibiting compounds in a Giardia lamblia high-throughput screen
    Rubén Bonilla-Santiago
    Tufts Cummings School of Veterinary Medicine, 200 Westboro Road, North Grafton, MA 01536, USA
    Mol Biochem Parasitol 162:149-54. 2008
    ..lamblia trophozoites. High-throughput screening of bioactive compounds will open new avenues to a system-wide analysis of pathways affecting G. lamblia proliferation, and eventually to other phases of the life cycle...
  37. pmc Cell sorting-assisted microarray profiling of host cell response to Cryptosporidium parvum infection
    Yi Lin Yang
    Tufts Cummings School of Veterinary Medicine, Division of Infectious Diseases, North Grafton, MA 01536, USA
    Infect Immun 78:1040-8. 2010
    ....
  38. pmc Over-expression and localization of a host protein on the membrane of Cryptosporidium parvum infected epithelial cells
    Yi Lin Yang
    Tufts Cummings School of Veterinary Medicine, Division of Infectious Diseases, 200 Westboro Road, North Grafton, MA 01536, USA
    Mol Biochem Parasitol 168:95-101. 2009
    ..This observation indicates that the composition of the host cell membrane is affected by the developing trophozoite, a phenomenon which has not been described previously...
  39. ncbi request reprint Cryptosporidium parvum in children with diarrhea in Mulago Hospital, Kampala, Uganda
    James K Tumwine
    Department of Pediatrics and Child Health, Mulago Hospital, Makerere University Medical School, Kampala, Uganda
    Am J Trop Med Hyg 68:710-5. 2003
    ..parvum. Among Ugandan children, cryptosporidiosis, which remains untreatable, is frequently associated with diarrhea and other serious and unfavorable consequences...
  40. ncbi request reprint Complete genome sequence of the apicomplexan, Cryptosporidium parvum
    Mitchell S Abrahamsen
    Department of Veterinary and Biomedical Science, College of Veterinary Medicine, University of Minnesota, St Paul, MN 55108, USA
    Science 304:441-5. 2004
    ..Elucidation of the core metabolism, including enzymes with high similarities to bacterial and plant counterparts, opens new avenues for drug development...
  41. ncbi request reprint The genome of Cryptosporidium hominis
    Ping Xu
    Center for the Study of Biological Complexity, Virginia Commonwealth University, Richmond, Virginia 23284 2030, USA
    Nature 431:1107-12. 2004
    ..C. hominis and C. parvum exhibit very similar gene complements, and phenotypic differences between these parasites must be due to subtle sequence divergence...
  42. ncbi request reprint Detection of infectious parasites in reclaimed water
    Debra E Huffman
    University of South Florida, College of Marine Science, St Petersburg 33701, USA
    Water Environ Res 78:2297-302. 2006
    ..None of the Cryptosporidium oocysts concentrated from the reclaimed water samples appeared to be infectious...
  43. ncbi request reprint Cryptosporidium hominis: experimental challenge of healthy adults
    Cynthia L Chappell
    The University of Texas Health Science Center at Houston, School of Public Health and Medical School, Houston, Texas 77030, USA
    Am J Trop Med Hyg 75:851-7. 2006
    ..Cryptosporidium hominis 10 oocysts) and is clinically is infectious for healthy adults (ID(50) = similar to C. parvum-induced illness. In contrast to C. parvum, C. hominis elicted a serum IgG response in most infected persons...
  44. ncbi request reprint Giardia cysts in tertiary-treated wastewater effluents: are they infective?
    April Garcia
    San Jose Creek Water Quality Laboratory, County Sanitation Districts of Los Angeles County, Whittier, CA 90601, USA
    Water Environ Res 74:541-4. 2002
    ..This study found that determination of cyst concentrations without viability or infectivity assessment may significantly overestimate the potential health risks associated with protozoan cysts in tertiary-treated wastewater effluents...
  45. pmc UV inactivation of Cryptosporidium hominis as measured in cell culture
    Anne M Johnson
    The Metropolitan Water District of Southern California, Water Quality Laboratory, 700 Moreno Avenue, La Verne, CA 91750, USA
    Appl Environ Microbiol 71:2800-2. 2005
    ..hominis oocysts displayed similar levels of infectivity and had the same sensitivity to UV light as C. parvum. Therefore, the water industry can be confident about extrapolating C. parvum UV disinfection data to C. hominis oocysts...

Research Grants13

  1. Genetics of Cryptosporidium parvum
    Giovanni Widmer; Fiscal Year: 2002
    ..We will test the following two working hypotheses: a) Virulence is determined by multiple genetic loci. b) QTL analysis can be used to identify genomic segments associated with virulence. ..
  2. Genetics of Cryptosporidium parvum
    Giovanni Widmer; Fiscal Year: 2009
    ..Such valleys will be identified by quantitatively genotyping a selected and a control progeny population with a panel of single nucleotide polymorphisms. ..
  3. Functional Genomics of Cryptosporidium parvum
    Giovanni Widmer; Fiscal Year: 2007
    ..parvum. A comparison of the host cell response to C. parvum type 1 and type 2 will be performed to probe the molecular basis of the observed difference in virulence between types. ..
  4. Genetics of Cryptosporidium parvum
    Giovanni Widmer; Fiscal Year: 2006
    ..We will test the following two working hypotheses: a) Virulence is determined by multiple genetic loci. b) QTL analysis can be used to identify genomic segments associated with virulence. ..
  5. Functional Genemics of Cryptosporidium parvum
    Giovanni Widmer; Fiscal Year: 2006
    ..parvum. A comparison of the host cell response to C. parvum type 1 and type 2 will be performed to probe the molecular basis of the observed difference in virulence between types. ..
  6. Functional Genemics of Cryptosporidium parvum
    Giovanni Widmer; Fiscal Year: 2005
    ..parvum. A comparison of the host cell response to C. parvum type 1 and type 2 will be performed to probe the molecular basis of the observed difference in virulence between types. ..
  7. Genetics of Cryptosporidium parvum
    Giovanni Widmer; Fiscal Year: 2005
    ..We will test the following two working hypotheses: a) Virulence is determined by multiple genetic loci. b) QTL analysis can be used to identify genomic segments associated with virulence. ..
  8. Genetic Transformation of Cryptosporidium parvum
    Giovanni Widmer; Fiscal Year: 2005
    ..Approach: Transformation by electroporation of sporozoites with GFP expression plasmids; detection of transformants in cell culture by fluorescent microscopy, anti-GFP antibodies, or reverse-transcriptase PCR. ..
  9. Functional Genomics of Cryptosporidium parvum
    Giovanni Widmer; Fiscal Year: 2004
    ..parvum. A comparison of the host cell response to C. parvum type 1 and type 2 will be performed to probe the molecular basis of the observed difference in virulence between types. ..
  10. Genetics of Cryptosporidium parvum
    Giovanni Widmer; Fiscal Year: 2004
    ..We will test the following two working hypotheses: a) Virulence is determined by multiple genetic loci. b) QTL analysis can be used to identify genomic segments associated with virulence. ..
  11. Genetics of Cryptosporidium parvum
    Giovanni Widmer; Fiscal Year: 2003
    ..We will test the following two working hypotheses: a) Virulence is determined by multiple genetic loci. b) QTL analysis can be used to identify genomic segments associated with virulence. ..
  12. Genetics of Cryptosporidium parvum
    Giovanni Widmer; Fiscal Year: 2010
    ..Such valleys will be identified by quantitatively genotyping a selected and a control progeny population with a panel of single nucleotide polymorphisms. ..