Charlotte Kuperwasser

Summary

Affiliation: Tufts University
Country: USA

Publications

  1. pmc Estrogen expands breast cancer stem-like cells through paracrine FGF/Tbx3 signaling
    Christine M Fillmore
    Sackler School of Graduate Biomedical Sciences, Tufts University School of Medicine, Boston, MA 02111, USA
    Proc Natl Acad Sci U S A 107:21737-42. 2010
  2. pmc Human breast cancer stem cell markers CD44 and CD24: enriching for cells with functional properties in mice or in man?
    Christine Fillmore
    Department of Genetics, Sackler School, Tufts University School of Medicine, Harrison Avenue, Boston, Massachusetts 02111, USA
    Breast Cancer Res 9:303. 2007
  3. pmc Functional heterogeneity of breast fibroblasts is defined by a prostaglandin secretory phenotype that promotes expansion of cancer-stem like cells
    Jenny A Rudnick
    Graduate Program in Cell, Molecular and Developmental Biology Program, Sackler School of Graduate Biomedical Sciences, Tufts University School of Medicine, Boston, Massachusetts, United States of America
    PLoS ONE 6:e24605. 2011
  4. pmc The evolving paradigm of tissue-specific metastasis
    Theresa A DiMeo
    Department of Anatomy and Cellular Biology, Tufts University School of Medicine, Boston, Massachusetts, USA
    Breast Cancer Res 8:301. 2006
  5. pmc Fibroblast-secreted hepatocyte growth factor mediates epidermal growth factor receptor tyrosine kinase inhibitor resistance in triple-negative breast cancers through paracrine activation of Met
    Kelly L Mueller
    Department of Oncology, Wayne State University, 4100 John R, Street, Detroit, MI 48201, USA
    Breast Cancer Res 14:R104. 2012
  6. pmc Reconstruction of functionally normal and malignant human breast tissues in mice
    Charlotte Kuperwasser
    Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, MA 02142, USA
    Proc Natl Acad Sci U S A 101:4966-71. 2004
  7. ncbi request reprint A mouse model of human breast cancer metastasis to human bone
    Charlotte Kuperwasser
    Department of Physiology, Tufts University School of Medicine, Boston, Massachusetts 02111, USA
    Cancer Res 65:6130-8. 2005
  8. ncbi request reprint Identification of selective inhibitors of cancer stem cells by high-throughput screening
    Piyush B Gupta
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Cell 138:645-59. 2009
  9. pmc Obesity promotes breast cancer by CCL2-mediated macrophage recruitment and angiogenesis
    Lisa M Arendt
    Authors Affiliations Developmental, Molecular, and Chemical Biology Department, Sackler School of Graduate Biomedical Sciences, Tufts University School of Medicine Molecular Oncology Research Institute and Medical Oncology and Department of Pathology, Tufts Medical Center, Boston, Massachusetts and Departments of Medicine, Duke University, Durham, North Carolina
    Cancer Res 73:6080-93. 2013
  10. pmc Distinct roles of the three Akt isoforms in lactogenic differentiation and involution
    Ioanna G Maroulakou
    Molecular Oncology Research Institute, Tufts Medical Center, Boston, Massachusetts, USA
    J Cell Physiol 217:468-77. 2008

Collaborators

Detail Information

Publications35

  1. pmc Estrogen expands breast cancer stem-like cells through paracrine FGF/Tbx3 signaling
    Christine M Fillmore
    Sackler School of Graduate Biomedical Sciences, Tufts University School of Medicine, Boston, MA 02111, USA
    Proc Natl Acad Sci U S A 107:21737-42. 2010
    ..These findings demonstrate that breast CSCs are stimulated by estrogen through a signaling pathway that similarly controls normal mammary epithelial stem cell biology...
  2. pmc Human breast cancer stem cell markers CD44 and CD24: enriching for cells with functional properties in mice or in man?
    Christine Fillmore
    Department of Genetics, Sackler School, Tufts University School of Medicine, Harrison Avenue, Boston, Massachusetts 02111, USA
    Breast Cancer Res 9:303. 2007
    ....
  3. pmc Functional heterogeneity of breast fibroblasts is defined by a prostaglandin secretory phenotype that promotes expansion of cancer-stem like cells
    Jenny A Rudnick
    Graduate Program in Cell, Molecular and Developmental Biology Program, Sackler School of Graduate Biomedical Sciences, Tufts University School of Medicine, Boston, Massachusetts, United States of America
    PLoS ONE 6:e24605. 2011
    ..Identifying markers of these cells could allow for the targeted ablation of tumor-promoting and inflammatory fibroblasts in human breast cancers...
  4. pmc The evolving paradigm of tissue-specific metastasis
    Theresa A DiMeo
    Department of Anatomy and Cellular Biology, Tufts University School of Medicine, Boston, Massachusetts, USA
    Breast Cancer Res 8:301. 2006
  5. pmc Fibroblast-secreted hepatocyte growth factor mediates epidermal growth factor receptor tyrosine kinase inhibitor resistance in triple-negative breast cancers through paracrine activation of Met
    Kelly L Mueller
    Department of Oncology, Wayne State University, 4100 John R, Street, Detroit, MI 48201, USA
    Breast Cancer Res 14:R104. 2012
    ..CONCLUSIONS: Taken together, these data suggest a role for Met in clinical resistance to EGFR TKIs in breast cancer through EGFR/Met crosstalk mediated by tumor-stromal interactions...
  6. pmc Reconstruction of functionally normal and malignant human breast tissues in mice
    Charlotte Kuperwasser
    Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, MA 02142, USA
    Proc Natl Acad Sci U S A 101:4966-71. 2004
    ..This experimental model allows for studies of human epithelial morphogenesis and differentiation in vivo and underscores the critical role of heterotypic interactions in human breast development and carcinogenesis...
  7. ncbi request reprint A mouse model of human breast cancer metastasis to human bone
    Charlotte Kuperwasser
    Department of Physiology, Tufts University School of Medicine, Boston, Massachusetts 02111, USA
    Cancer Res 65:6130-8. 2005
    ..This model replicates the events observed in patients with breast cancer skeletal metastases and serves as a useful and relevant model for studying the disease...
  8. ncbi request reprint Identification of selective inhibitors of cancer stem cells by high-throughput screening
    Piyush B Gupta
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Cell 138:645-59. 2009
    ..This study demonstrates the ability to identify agents with specific toxicity for epithelial CSCs...
  9. pmc Obesity promotes breast cancer by CCL2-mediated macrophage recruitment and angiogenesis
    Lisa M Arendt
    Authors Affiliations Developmental, Molecular, and Chemical Biology Department, Sackler School of Graduate Biomedical Sciences, Tufts University School of Medicine Molecular Oncology Research Institute and Medical Oncology and Department of Pathology, Tufts Medical Center, Boston, Massachusetts and Departments of Medicine, Duke University, Durham, North Carolina
    Cancer Res 73:6080-93. 2013
    ..These findings provide a mechanistic role for adipocytes and macrophages before carcinogenesis that may be critical for prevention and treatment of obesity-related cancer...
  10. pmc Distinct roles of the three Akt isoforms in lactogenic differentiation and involution
    Ioanna G Maroulakou
    Molecular Oncology Research Institute, Tufts Medical Center, Boston, Massachusetts, USA
    J Cell Physiol 217:468-77. 2008
    ..We conclude that the three Akt isoforms have different roles in mammary gland differentiation during pregnancy and this may reflect differences in hormonal signaling...
  11. doi request reprint Stochastic state transitions give rise to phenotypic equilibrium in populations of cancer cells
    Piyush B Gupta
    Broad Institute, Cambridge, MA 02142, USA
    Cell 146:633-44. 2011
    ..These findings contribute to our understanding of cancer heterogeneity and reveal how stochasticity in single-cell behaviors promotes phenotypic equilibrium in populations of cancer cells...
  12. pmc Human breast cancer cell lines contain stem-like cells that self-renew, give rise to phenotypically diverse progeny and survive chemotherapy
    Christine M Fillmore
    Department of Genetics, Sackler School, Tufts University School of Medicine, Harrison Avenue, Boston, Massachusetts 02111, USA
    Breast Cancer Res 10:R25. 2008
    ..We embarked on this study hypothesizing that breast cancer cell lines would contain analogous hierarchical differentiation programs to those found in primary breast tumors...
  13. pmc Mapping the cellular and molecular heterogeneity of normal and malignant breast tissues and cultured cell lines
    Patrica J Keller
    Department of Anatomy and Cellular Biology, Sackler School, Tufts University School of Medicine, Boston, MA 02111, USA
    Breast Cancer Res 12:R87. 2010
    ..While cultured cell lines have been derived from both normal and malignant tissues, it remains unclear whether they retain a similar cellular heterogeneity as to that found within breast tissues...
  14. pmc Genetic predisposition directs breast cancer phenotype by dictating progenitor cell fate
    Theresa A Proia
    Department of Anatomy and Cellular Biology, Sackler School of Biomedical Research, Tufts University School of Medicine, 136 Harrison Ave, Boston, MA 02111, USA
    Cell Stem Cell 8:149-63. 2011
    ..These findings demonstrate that the genetic background of patient populations, in addition to affecting incidence rates, significantly impacts progenitor cell fate commitment and, therefore, tumor phenotype...
  15. pmc Cyclin D1 activity regulates autophagy and senescence in the mammary epithelium
    Nelson E Brown
    Molecular Oncology Research Institute, Tufts Medical Center, Boston, Massachusetts 02111, USA
    Cancer Res 72:6477-89. 2012
    ..Collectively, our findings suggest a previously unrecognized function of cyclin D1 in suppressing autophagy in the mammary epithelium...
  16. pmc Cyclin D1 kinase activity is required for the self-renewal of mammary stem and progenitor cells that are targets of MMTV-ErbB2 tumorigenesis
    Rinath Jeselsohn
    Molecular Oncology Research Institute, Tufts Medical Center, Boston, MA 02111, USA
    Cancer Cell 17:65-76. 2010
    ....
  17. ncbi request reprint Systemic stromal effects of estrogen promote the growth of estrogen receptor-negative cancers
    Piyush B Gupta
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA
    Cancer Res 67:2062-71. 2007
    ..Furthermore, bone marrow cells from estrogen-treated mice were sufficient to promote tumor growth. These results reveal a novel mechanism by which estrogens promote the growth of ER-negative cancers...
  18. pmc Stroma in breast development and disease
    Lisa M Arendt
    Department of Anatomy and Cellular Biology, Sackler School, Tufts University School of Medicine, Boston, MA 02111, USA
    Semin Cell Dev Biol 21:11-8. 2010
    ..Recognition of the important role of the stroma during the progression of breast cancers leads to the possibility of new targets for treatment of the initial breast cancer lesion as well as prevention of recurrence...
  19. pmc Defining the cellular precursors to human breast cancer
    Patricia J Keller
    Department of Anatomy and Cellular Biology, Sackler School of Graduate Biomedical Sciences, Tufts University School of Medicine, Boston, MA 02111, USA
    Proc Natl Acad Sci U S A 109:2772-7. 2012
    ..These findings identify normal cellular precursors to human breast cancers and reveal the existence of a population of cells with epidermal progenitor activity within adult human breast tissues...
  20. pmc A novel lung metastasis signature links Wnt signaling with cancer cell self-renewal and epithelial-mesenchymal transition in basal-like breast cancer
    Theresa A DiMeo
    Department of Anatomy and Cellular Biology, Sackler School, Tufts University School of Medicine, Boston, MA 02111, USA
    Cancer Res 69:5364-73. 2009
    ..Collectively, these results provide a molecular link between self-renewal, EMT, and metastasis in basal-like breast cancers...
  21. pmc The RasGAP gene, RASAL2, is a tumor and metastasis suppressor
    Sara Koenig McLaughlin
    Genetics Division, Brigham and Women s Hospital, Boston, MA 02115, USA Department of Medicine, Brigham and Women s Hospital, Boston, MA 02115, USA Harvard Medical School, Boston, MA 02115, USA
    Cancer Cell 24:365-78. 2013
    ..Additional data reveal a broader role for RASAL2 inactivation in other tumor types. These studies highlight the expanding role of RasGAPs and reveal an alternative mechanism of activating Ras in cancer. ..
  22. doi request reprint Stromal biomarkers in breast cancer development and progression
    Jenny A Rudnick
    Department of Anatomy and Cellular Biology, Sackler School of Graduate Biomedical Sciences, Tufts University School of Medicine, 136 Harrison Ave, Boston, MA 02111, USA
    Clin Exp Metastasis 29:663-72. 2012
    ..Characterizing these stromal changes will facilitate their use as clinical biomarkers in breast cancer, and may facilitate their use as potential drug targets for adjuvant breast cancer therapy...
  23. ncbi request reprint Estrogen promotes ER-negative tumor growth and angiogenesis through mobilization of bone marrow-derived monocytes
    Vandana Iyer
    Department of Anatomy and Cellular Biology, Sackler School of Biomedical Research, Tufts Medical Center, Boston, Massachusetts 02111, USA
    Cancer Res 72:2705-13. 2012
    ..Our findings reveal a novel way in which estrogen promotes tumor formation, with implications for the development and application of anti-estrogen therapies to treat cancer in premenopausal women...
  24. pmc Dissecting genetic requirements of human breast tumorigenesis in a tissue transgenic model of human breast cancer in mice
    Min Wu
    AVEO Pharmaceuticals Inc, 75 Sidney Street, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 106:7022-7. 2009
    ..The HIM model is an experimentally tractable human in vivo system that holds great potential for advancing our basic understanding of cancer biology and for the discovery and testing of targeted therapies...
  25. pmc The contribution of dynamic stromal remodeling during mammary development to breast carcinogenesis
    Jessica McCready
    Department of Anatomy and Cellular Biology, Sackler School, Tufts University School of Medicine, 136 Harrison Ave, Boston, MA 02111, USA
    Breast Cancer Res 12:205. 2010
    ....
  26. pmc The melanocyte differentiation program predisposes to metastasis after neoplastic transformation
    Piyush B Gupta
    Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA
    Nat Genet 37:1047-54. 2005
    ..Moreover, Slug is required for the metastasis of the transformed melanoma cells. These findings indicate that melanocyte-specific factors present before neoplastic transformation can have a pivotal role in governing melanoma progression...
  27. pmc Normal and neoplastic nonstem cells can spontaneously convert to a stem-like state
    Christine L Chaffer
    Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA
    Proc Natl Acad Sci U S A 108:7950-5. 2011
    ..The observed plasticity may allow derivation of patient-specific adult stem cells without genetic manipulation and holds important implications for therapeutic strategies to eradicate cancer...
  28. pmc Endothelial Akt signaling is rate-limiting for rapamycin inhibition of mouse mammary tumor progression
    Thuy L Phung
    Department of Pathology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA
    Cancer Res 67:5070-5. 2007
    ..Together, these data support the hypothesis that endothelial Akt signaling in the tumor vasculature is an important target of the novel anticancer drug rapamycin...
  29. ncbi request reprint Alterations of the HBP1 transcriptional repressor are associated with invasive breast cancer
    K Eric Paulson
    Department of Biochemistry and Program in Genetics, Tufts University School of Medicine, Boston, MA 02111, USA
    Cancer Res 67:6136-45. 2007
    ..Together, these data indicate that HBP1 may be a molecularly and clinically relevant regulator of breast cancer transitions that eventually lead to poor prognosis...
  30. pmc The Hippo transducer TAZ interacts with the SWI/SNF complex to regulate breast epithelial lineage commitment
    Adam Skibinski
    Department of Developmental, Chemical, and Molecular Biology, Tufts University, 145 Harrison Avenue, Boston, MA 02111, USA Molecular Oncology Research Institute, Tufts Medical Center, 800 Washington Street, Boston, MA 02111, USA
    Cell Rep 6:1059-72. 2014
    ..Collectively, these findings uncover a new role for Hippo signaling in the determination of lineage identity through recruitment of chromatin-remodeling complexes. ..
  31. ncbi request reprint Reconstruction of human mammary tissues in a mouse model
    David A Proia
    Department of Anatomy and Cellular Biology, Tufts University School of Medicine, 136 Harrison Ave, Boston, Massachusetts 02111, USA
    Nat Protoc 1:206-14. 2006
    ..As a whole, this model system has the potential to improve the understanding of crosstalk between tissue stroma and the epithelium as well as factors involved in breast stem cell biology tumor initiation and progression...
  32. ncbi request reprint NDY1/KDM2B Functions as a Master Regulator of Polycomb Complexes and Controls Self-Renewal of Breast Cancer Stem Cells
    Filippos Kottakis
    Authors Affiliation Molecular Oncology Research Institute, Tufts Medical Center, Boston, Massachusetts
    Cancer Res 74:3935-46. 2014
    ..Finally, in primary human breast cancer, NDY1/KDM2B expression correlates negatively with the expression of the NDY1-regulated miRNAs and positively with the expression of their PRC targets. Cancer Res; 74(14); 3935-46. ©2014 AACR. ..
  33. ncbi request reprint Contributions of estrogen to ER-negative breast tumor growth
    Piyush B Gupta
    Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, MA 02142, USA
    J Steroid Biochem Mol Biol 102:71-8. 2006
    ..Taken together, these data suggest that estrogen may promote the growth of ER-negative cancers by acting on cells distinct from the cancer cells to stimulate angiogenesis...
  34. ncbi request reprint Stem cell maintenance of the mammary gland: it takes two
    Patricia J Keller
    Department of Anatomy and Cellular Biology, Sackler School, Tufts University School of Medicine, Boston, MA 02111, USA Molecular Oncology Research Institute, Tufts Medical Center, Boston, MA 02111, USA
    Cell Stem Cell 9:496-7. 2011
    ..Recently in Nature, Van Keymeulen et al. (2011) used lineage tracing to discover unipotent stem cells that maintain the bulk of the mouse mammary gland after birth and during pregnancy...
  35. pmc Portable two-color in vivo flow cytometer for real-time detection of fluorescently-labeled circulating cells
    Steven Boutrus
    Tufts University, Biomedical Engineering Department, Medford, Massachusetts 02155, USA
    J Biomed Opt 12:020507. 2007
    ....

Research Grants5

  1. Mechanisms of estrogen action on bone marrow-derived stem stromal cells
    Charlotte Kuperwasser; Fiscal Year: 2007
    ..Thus, understanding the mechanism by which estrogen can promote bone marrow cell recruitment, angiogenesis, and tumor growth would have significant and highly relevant scientific and clinical impact for ER-negative cancers. ..
  2. Mechanisms of estrogen action on bone marrow-derived stem stromal cells
    Charlotte Kuperwasser; Fiscal Year: 2009
    ..abstract_text> ..
  3. Mechanisms of estrogen action on bone marrow-derived stem stromal cells
    Charlotte Kuperwasser; Fiscal Year: 2010
    ..abstract_text> ..
  4. Mechanisms of estrogen action on bone marrow-derived stem stromal cells
    Charlotte Kuperwasser; Fiscal Year: 2011
    ..abstract_text> ..