Affiliation: Tufts University
Freudenreich C. R-loops: targets for nuclease cleavage and repeat instability. Curr Genet. 2018;64:789-794 pubmed publisher
..This perspective summarizes mechanisms of R-loop-induced fragility at G-rich repeats and new links between DNA breaks and repeat instability. ..
Koch M, House N, Cosetta C, Jong R, Salomon C, Joyce C, et al
. The Chromatin Remodeler Isw1 Prevents CAG Repeat Expansions During Transcription in Saccharomyces cerevisiae. Genetics. 2018;208:963-976 pubmed publisher
..These results establish a new function for Isw1 in directly maintaining the chromatin structure at the CAG repeat, thereby limiting expansions that can occur during transcription-coupled excision repair. ..
Freudenreich C. Chromosome fragility: molecular mechanisms and cellular consequences. Front Biosci. 2007;12:4911-24 pubmed
..An understanding of these events will provide insight into the generation of cancer, since deletions and rearrangements at human common fragile sites and associated tumor suppressor genes are an early event in tumorigenesis...
House N, Yang J, Walsh S, Moy J, Freudenreich C. NuA4 initiates dynamic histone H4 acetylation to promote high-fidelity sister chromatid recombination at postreplication gaps. Mol Cell. 2014;55:818-828 pubmed publisher
..Here we have utilized a repetitive DNA sequence that induces endogenous DNA damage to identify histone modifications that regulate recombination efficiency and fidelity during postreplication gap repair. ..
Su X, Dion V, Gasser S, Freudenreich C. Regulation of recombination at yeast nuclear pores controls repair and triplet repeat stability. Genes Dev. 2015;29:1006-17 pubmed publisher
..We thereby confirmed that the relocation of damage to nuclear pores plays an important role in a naturally occurring repair process. ..
Polleys E, House N, Freudenreich C. Role of recombination and replication fork restart in repeat instability. DNA Repair (Amst). 2017;56:156-165 pubmed publisher
..In this review, we summarize recent developments that illuminate the role of recombination in maintaining genome stability at DNA repeats. ..
Su X, Freudenreich C. Cytosine deamination and base excision repair cause R-loop-induced CAG repeat fragility and instability in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 2017;114:E8392-E8401 pubmed publisher
..Since disease-causing CAG repeats occur in transcribed regions, our results suggest that R-loop-mediated fragility is a mechanism that could cause DNA damage and repeat-length changes in human cells. ..
Polleys E, Freudenreich C. Methods to Study Repeat Fragility and Instability in Saccharomyces cerevisiae. Methods Mol Biol. 2018;1672:403-419 pubmed publisher
..All three assays have been essential in understanding the genetic mechanisms that cause chromosome breaks and tract-length changes at unstable repeats. ..