Diana W Bianchi

Summary

Affiliation: Tufts Medical Center
Country: USA

Publications

  1. doi request reprint From prenatal genomic diagnosis to fetal personalized medicine: progress and challenges
    Diana W Bianchi
    The Mother Infant Research Institute at Tufts Medical Center and the Division of Genetics, Department of Pediatrics, Floating Hospital for Children, Boston, Massachusetts, USA
    Nat Med 18:1041-51. 2012
  2. pmc Increased incidence of pregnancy complications in women who later develop scleroderma: a case control study
    Linda van Wyk
    Department of Obstetrics, Leiden University Medical Centre, Albinusdreef 2, 2333ZA Leiden, PO Box 9600, 2300 RC Leiden, The Netherlands
    Arthritis Res Ther 13:R183. 2011
  3. pmc Insights into fetal and neonatal development through analysis of cell-free RNA in body fluids
    Diana W Bianchi
    Mother Infant Research Institute at Tufts Medical Center, Department of Pediatrics, Floating Hospital for Children at Tufts Medical Center, Boston, MA 02111, USA
    Early Hum Dev 86:747-52. 2010
  4. ncbi request reprint Cell-free fetal DNA in amniotic fluid: unique fragmentation signatures in euploid and aneuploid fetuses
    Olav Lapaire
    Department of Pediatrics, Tufts New England Medical Center, Boston, MA 02111, USA
    Clin Chem 53:405-11. 2007
  5. ncbi request reprint Circulating cell-free fetal nucleic acid analysis may be a novel marker of fetomaternal hemorrhage after elective first-trimester termination of pregnancy
    Tuangsit Wataganara
    Division of Genetics, Department of Pediatrics, Tufts New England Medical Center, Boston, Massachusetts 02111, USA
    Ann N Y Acad Sci 1022:129-34. 2004
  6. pmc Increased fetal cell trafficking in murine lung following complete pregnancy loss from exposure to lipopolysaccharide
    Kirby L Johnson
    Division of Genetics, Department of Pediatrics, Floating Hospital for Children at Tufts Medical Center, 800 Washington Street, Box 394, Boston, MA 02111, USA
    Fertil Steril 93:1718-1721.e2. 2010
  7. pmc Fetal cells in the pregnant mouse are diverse and express a variety of progenitor and differentiated cell markers
    Yutaka Fujiki
    Division of Genetics, Department of Pediatrics, Floating Hospital for Children at Tufts Medical Center, Boston, Massachusetts 02111, USA
    Biol Reprod 81:26-32. 2009
  8. pmc Novel neurodevelopmental information revealed in amniotic fluid supernatant transcripts from fetuses with trisomies 18 and 21
    Lisa Hui
    Mother Infant Research Institute and the Division of Genetics, Department of Pediatrics, The Floating Hospital for Children at Tufts Medical Center, 800 Washington St, Boston, MA 02111, USA
    Hum Genet 131:1751-9. 2012
  9. ncbi request reprint Fetal nucleic acids in maternal body fluids: an update
    Diana W Bianchi
    Tufts New England Medical Center, Box 394, 750 Washington Street, Boston, MA 02111, USA
    Ann N Y Acad Sci 1075:63-73. 2006
  10. pmc Relationships between cell-free DNA and serum analytes in the first and second trimesters of pregnancy
    Neeta L Vora
    Division of Maternal Fetal Medicine, Department of Obstetrics and Gynecology, Tufts Medical Center, Boston, Massachusetts, USA
    Obstet Gynecol 116:673-8. 2010

Detail Information

Publications86

  1. doi request reprint From prenatal genomic diagnosis to fetal personalized medicine: progress and challenges
    Diana W Bianchi
    The Mother Infant Research Institute at Tufts Medical Center and the Division of Genetics, Department of Pediatrics, Floating Hospital for Children, Boston, Massachusetts, USA
    Nat Med 18:1041-51. 2012
    ..We are now at a point where that goal can and should be expanded to incorporate genetic, genomic and transcriptomic data to develop new approaches to fetal treatment...
  2. pmc Increased incidence of pregnancy complications in women who later develop scleroderma: a case control study
    Linda van Wyk
    Department of Obstetrics, Leiden University Medical Centre, Albinusdreef 2, 2333ZA Leiden, PO Box 9600, 2300 RC Leiden, The Netherlands
    Arthritis Res Ther 13:R183. 2011
    ..From these prior studies we hypothesized that preeclampsia and other pregnancy complications could be associated with increased levels of fetal cell trafficking, and later be involved in the development of scleroderma...
  3. pmc Insights into fetal and neonatal development through analysis of cell-free RNA in body fluids
    Diana W Bianchi
    Mother Infant Research Institute at Tufts Medical Center, Department of Pediatrics, Floating Hospital for Children at Tufts Medical Center, Boston, MA 02111, USA
    Early Hum Dev 86:747-52. 2010
    ..We also discuss the ways in which differentially-regulated gene lists can advance knowledge of both fetal and neonatal biology, as well as suggest novel possibilities for fetal and neonatal treatment...
  4. ncbi request reprint Cell-free fetal DNA in amniotic fluid: unique fragmentation signatures in euploid and aneuploid fetuses
    Olav Lapaire
    Department of Pediatrics, Tufts New England Medical Center, Boston, MA 02111, USA
    Clin Chem 53:405-11. 2007
    ..Amniotic fluid (AF) is a rich source of cffDNA. We investigated the biophysical characteristics of cffDNA in AF, hypothesizing that they would differ from cffDNA in maternal plasma...
  5. ncbi request reprint Circulating cell-free fetal nucleic acid analysis may be a novel marker of fetomaternal hemorrhage after elective first-trimester termination of pregnancy
    Tuangsit Wataganara
    Division of Genetics, Department of Pediatrics, Tufts New England Medical Center, Boston, Massachusetts 02111, USA
    Ann N Y Acad Sci 1022:129-34. 2004
    ..Measurement of cell-free fetal nucleic acid levels in maternal plasma may have clinical application as a novel marker of FMH after 9 weeks of gestation...
  6. pmc Increased fetal cell trafficking in murine lung following complete pregnancy loss from exposure to lipopolysaccharide
    Kirby L Johnson
    Division of Genetics, Department of Pediatrics, Floating Hospital for Children at Tufts Medical Center, 800 Washington Street, Box 394, Boston, MA 02111, USA
    Fertil Steril 93:1718-1721.e2. 2010
    ....
  7. pmc Fetal cells in the pregnant mouse are diverse and express a variety of progenitor and differentiated cell markers
    Yutaka Fujiki
    Division of Genetics, Department of Pediatrics, Floating Hospital for Children at Tufts Medical Center, Boston, Massachusetts 02111, USA
    Biol Reprod 81:26-32. 2009
    ..Future studies will address whether fetal cells cross the placental barrier in a differentiated state or as a homogenous population and subsequently differentiate in target maternal organs...
  8. pmc Novel neurodevelopmental information revealed in amniotic fluid supernatant transcripts from fetuses with trisomies 18 and 21
    Lisa Hui
    Mother Infant Research Institute and the Division of Genetics, Department of Pediatrics, The Floating Hospital for Children at Tufts Medical Center, 800 Washington St, Boston, MA 02111, USA
    Hum Genet 131:1751-9. 2012
    ..Our results show that whole transcriptome analysis of cell-free RNA in AF from live pregnancies permits discovery of biomarkers of abnormal human neurodevelopment and advances our understanding of the pathophysiology of aneuploidy...
  9. ncbi request reprint Fetal nucleic acids in maternal body fluids: an update
    Diana W Bianchi
    Tufts New England Medical Center, Box 394, 750 Washington Street, Boston, MA 02111, USA
    Ann N Y Acad Sci 1075:63-73. 2006
    ..On the other hand, we are cautious and concerned about the accuracy of "at-home" kits for fetal gender detection...
  10. pmc Relationships between cell-free DNA and serum analytes in the first and second trimesters of pregnancy
    Neeta L Vora
    Division of Maternal Fetal Medicine, Department of Obstetrics and Gynecology, Tufts Medical Center, Boston, Massachusetts, USA
    Obstet Gynecol 116:673-8. 2010
    ..To assess the relationship between first- and second-trimester cell-free DNA levels and maternal serum screening markers...
  11. ncbi request reprint Array-CGH analysis of cell-free fetal DNA in 10 mL of amniotic fluid supernatant
    Olav Lapaire
    Division of Genetics, Department of Pediatrics, Tufts New England Medical Center, Boston, MA, USA
    Prenat Diagn 27:616-21. 2007
    ..Subsequent technical advances have increased both the yield and quality of extracted cffDNA. Here we determined whether array-CGH using smaller volumes of both fresh and frozen AF cffDNA could identify fetal aneuploidy...
  12. ncbi request reprint Circulating cell-free fetal messenger RNA levels after fetoscopic interventions of complicated pregnancies
    May Lee Tjoa
    Department of Pediatrics, Division of Genetics, Tufts New England Medical Center, Boston, MA 02111, USA
    Am J Obstet Gynecol 195:230-5. 2006
    ..The aim of this study was to examine fetal gene expression in maternal plasma after fetoscopic intervention for twin-twin transfusion syndrome or congenital diaphragmatic hernia...
  13. ncbi request reprint Cell-free DNA fragmentation patterns in amniotic fluid identify genetic abnormalities and changes due to storage
    Inga Peter
    Institute for Clinical Research and Health Policy Studies, Tufts New England Medical Center, 750 Washington St NEMC no 63, Boston, MA 02111, USA
    Diagn Mol Pathol 17:185-90. 2008
    ....
  14. ncbi request reprint ROC analysis of an erythroblast morphologic scoring system to improve identification of fetal cells in maternal blood
    Dong Hyun Cha
    Departments of Obstetrics and Gynecology and Pediatrics, Tufts New England Medical Center, Boston, USA
    Prenat Diagn 24:117-20. 2004
    ..Recently, we developed an erythroblast scoring system based on these characteristics. Here, we employ statistical analyses to further characterize the utility of this scoring system...
  15. doi request reprint Performing discovery-driven neonatal research by transcriptomic analysis of routinely discarded biofluids
    Jill L Maron
    Department of Pediatrics, The Floating Hospital for Children at Tufts Medical Center, Boston, MA 02111, USA
    J Matern Fetal Neonatal Med 25:2507-11. 2012
    ..To perform discovery-driven research on the neonatal salivary and cord blood transcriptomes...
  16. ncbi request reprint Changes of cell-free fetal DNA in maternal plasma after elective termination of pregnancy
    Tuangsit Wataganara
    Division of Genetics, Department of Pediatrics, Tufts New England Medical Center, Boston, MA 02111, USA
    Clin Chem 51:217-9. 2005
  17. ncbi request reprint Transfer of fetal cells with multilineage potential to maternal tissue
    Kiarash Khosrotehrani
    Department of Pediatrics, Tufts New England Medical Center, Boston, Mass 02111, USA
    JAMA 292:75-80. 2004
    ..Many studies have confirmed the residual presence of fetal cells in maternal blood and tissues following pregnancy. Fetal cells may respond to maternal injury by developing multilineage capacity in maternal organs...
  18. pmc Fetomaternal trafficking in the mouse increases as delivery approaches and is highest in the maternal lung
    Yutaka Fujiki
    Department of Pediatrics, Floating Hospital for Children at Tufts Medical Center, Boston, Massachusetts 02111, USA
    Biol Reprod 79:841-8. 2008
    ..Using a murine model, our data demonstrate that fetomaternal cellular trafficking occurs in reproducible patterns, is maximal near term delivery, and has predilection for the maternal lung...
  19. ncbi request reprint Cell-free fetal DNA levels in pregnancies conceived by IVF
    Phillip D Pan
    Division of Genetics, Department of Pediatricsogy, Tufts New England Medical Center, 750 Washington Street, Box 394, Boston, MA 02111, USA
    Hum Reprod 20:3152-6. 2005
    ..Increased levels of cell-free fetal DNA (cffDNA) in maternal plasma have been correlated with increased HCG levels. Our aim was to determine whether cffDNA levels are elevated in IVF pregnancies compared with natural pregnancies...
  20. ncbi request reprint Fetal cell-free nucleic acids in the maternal circulation: new clinical applications
    Tuangsit Wataganara
    Division of Genetics, Department of Pediatrics, Tufts New England Medical Center and Tufts University School of Medicine, Boston, Massachusetts 02111, USA
    Ann N Y Acad Sci 1022:90-9. 2004
    ..Widespread incorporation of fetal nucleic acid measurement into routine prenatal care depends on the identification of a readily accessible gender-independent fetal marker...
  21. ncbi request reprint The influence of fetal loss on the presence of fetal cell microchimerism: a systematic review
    Kiarash Khosrotehrani
    Tufts New England Medical Center, Boston, Massachusetts 02111, USA
    Arthritis Rheum 48:3237-41. 2003
    ..The objective of this study was to determine whether pregnancy history influences the persistence of fetal microchimeric cells...
  22. pmc Proof of concept study to assess fetal gene expression in amniotic fluid by nanoarray PCR
    Lauren J Massingham
    Division of Genetics, Department of Pediatrics, Floating Hospital for Children at Tufts Medical Center, Boston, Massachusetts, USA
    J Mol Diagn 13:565-70. 2011
    ..In the future, this technique could be optimized to examine specific genes instrumental in fetal organ system function, which could be a useful addition to prenatal care...
  23. pmc Global gene expression analysis of amniotic fluid cell-free RNA from recipient twins with twin-twin transfusion syndrome
    Lisa Hui
    Mother Infant Research Institute, Tufts Medical Center, Boston, MA, USA
    Prenat Diagn 33:873-83. 2013
    ..The objective of this study was to understand the biological pathways involved in twin-twin transfusion syndrome (TTTS) by performing global gene expression analysis of amniotic fluid (AF) cell-free RNA...
  24. doi request reprint Smoking in pregnancy is associated with increased total maternal serum cell-free DNA levels
    Adam C Urato
    Division of Maternal Fetal Medicine, Department of Obstetrics and Gynecology, Tufts New England Medical Center, Boston, MA 02111, USA
    Prenat Diagn 28:186-90. 2008
    ..Cell-free DNA is a marker of cellular apoptosis and necrosis. We wished to determine if maternal smoking affects maternal and fetal serum cell-free DNA levels...
  25. pmc Comprehensive analysis of genes expressed by rare microchimeric fetal cells in the maternal mouse lung
    Stephanie Pritchard
    Mother Infant Research Institute, Floating Hospital at Tufts Medical Center, Boston, Massachusetts, USA
    Biol Reprod 87:42. 2012
    ..Furthermore, the presence and persistence of mesenchymal stem cells in maternal organs may have implications for long-term postpartum maternal health...
  26. pmc The amniotic fluid transcriptome: a source of novel information about human fetal development
    Lisa Hui
    Mother Infant Research Institute and Division of Genetics, Department of Pediatrics, The Floating Hospital for Children, Tufts Medical Center, Boston, Massachusetts, USA
    Obstet Gynecol 119:111-8. 2012
    ..The objective of this study was to develop an understanding of the amniotic fluid core transcriptome by analyzing the transcripts ubiquitously present in the amniotic fluid supernatant of euploid midtrimester fetuses...
  27. pmc Optimal techniques for mRNA extraction from neonatal salivary supernatant
    Jessica A Dietz
    Division of Newborn Medicine, Department of Pediatrics, The Floating Hospital for Children at Tufts Medical Center, Medford, MA, USA
    Neonatology 101:55-60. 2012
    ..However, existing technologies prevent RNA extraction of small quantities found in neonatal salivary supernatant...
  28. pmc Gene expression analysis in pregnant women and their infants identifies unique fetal biomarkers that circulate in maternal blood
    Jill L Maron
    Department of Pediatrics, Tufts New England Medical Center, Boston, Massachusetts, USA
    J Clin Invest 117:3007-19. 2007
    ..Transcriptional analysis of maternal whole blood identifies a unique set of biologically diverse fetal genes and has a multitude of clinical applications...
  29. ncbi request reprint Cervical cancer and microchimerism
    Donghyun Cha
    Division of Genetics, Departments of Obstetrics and Gynecology and Pediatrics, Tufts New England Medical Center, Boston, Massachusetts 02111, USA
    Obstet Gynecol 102:774-81. 2003
    ..To determine whether microchimerism is involved in the pathogenesis or progression of cervical cancer...
  30. ncbi request reprint Prenatal diagnosis using cell-free nucleic acids in maternal body fluids: a decade of progress
    Jill L Maron
    Department of Pesiatrics, Tufts New England Center, Boston, MA 02111, USA
    Am J Med Genet C Semin Med Genet 145:5-17. 2007
    ..Prenatal diagnosis is poised to evolve from detection of aneuploidy to detection of deviation from normal development, which should provide novel opportunities for fetal treatment...
  31. pmc Quantification of green fluorescent protein by in vivo imaging, PCR, and flow cytometry: comparison of transgenic strains and relevance for fetal cell microchimerism
    Yutaka Fujiki
    Division of Genetics, Department of Pediatrics, Tufts New England Medical Center, Boston, Massachusetts, USA
    Cytometry A 73:11-118. 2008
    ....
  32. ncbi request reprint Cell-free fetal DNA levels in maternal plasma after elective first-trimester termination of pregnancy
    Tuangsit Wataganara
    Department of Pediatrics, Tufts New England Medical Center, Boston, Massachusetts 02111, USA
    Fertil Steril 81:638-44. 2004
    ..To determine if first-trimester elective termination of pregnancy affects cell-free fetal DNA (fDNA) levels in maternal plasma...
  33. ncbi request reprint Short-term clearance of cell-free nucleic acids after first-trimester termination of pregnancy
    Young Ju Jeong
    Division of Genetics, Department of Pediatrics, Tufts New England Medical Center, Boston, Massachusetts 02111, USA
    Fertil Steril 88:730-3. 2007
    ....
  34. ncbi request reprint Global gene expression analysis of the living human fetus using cell-free messenger RNA in amniotic fluid
    Paige B Larrabee
    Department of Pediatrics, Division of Newborn Medicine, Tufts New England Medical Center, Boston, Mass 02111, USA
    JAMA 293:836-42. 2005
    ..No molecular biological tests are available to monitor the ongoing development of human fetuses in vivo...
  35. pmc Down syndrome serum screening also identifies an increased risk for multicystic dysplastic kidney, two-vessel cord, and hydrocele
    Jodi D Hoffman
    Tufts Medical Center, Boston, MA 02111, USA
    Prenat Diagn 28:1204-8. 2008
    ..The FASTER trial compared first and second trimester screening methods for aneuploidy. We examined relationships between maternal serum markers and common congenital anomalies in the pediatric outcome data set of 36 837 subjects...
  36. ncbi request reprint Robert E. Gross Lecture. Fetomaternal cell trafficking: a story that begins with prenatal diagnosis and may end with stem cell therapy
    Diana W Bianchi
    Division of Genetics, Department of Pediatrics, The Floating Hospital for Children and Tufts New England Medical Center, Boston, MA 02111, USA
    J Pediatr Surg 42:12-8. 2007
  37. pmc The natural history of fetal cells in postpartum murine maternal lung and bone marrow: a two-stage phenomenon
    Stephanie Pritchard
    Mother Infant Research Institute, Floating Hospital at Tufts Medical Center, and Genetics Program, Department of Pathology, Sackler School of Graduate Biomedical Sciences, Boston, MA, USA
    Chimerism 3:59-64. 2012
    ..The results presented here will inform the design of future experiments and may have implications for women's health...
  38. pmc Fetal cells in the murine maternal lung have well-defined characteristics and are preferentially located in alveolar septum
    Kirby L Johnson
    Mother Infant Research Institute at Tufts Medical Center, Boston, Massachusetts 02111, USA
    Stem Cells Dev 21:158-65. 2012
    ..Nevertheless, this naturally acquired population of fetal cells in maternal lung should be considered in studies of lung biology and repair...
  39. ncbi request reprint Cell-free fetal DNA plasma extraction and real-time polymerase chain reaction quantification
    Jill L Maron
    Department of Obstetrics and Gynecology, Tufts University School of Medicine, Tufts New England Medical Center, Boston, MA, USA
    Methods Mol Med 132:51-63. 2007
    ....
  40. pmc Circulating cell-free DNA levels increase variably following chorionic villus sampling
    Neeta L Vora
    Division of Maternal Fetal Medicine, Department of Obstetrics and Gynecology, Tufts Medical Center, Boston, MA 02111, USA
    Prenat Diagn 30:325-8. 2010
    ..Cell-free fetal DNA (cffDNA) in maternal plasma results from degradation of fetal and/or placental cells. Our objective was to determine if chorionic villus sampling (CVS) causes increased release of fetal and/or maternal DNA...
  41. pmc Method for extraction of high-quantity and -quality cell-free DNA from amniotic fluid
    Olav Lapaire
    Division of Genetics, Department of Pediatrics, Tufts New England Medical Center and Floating Hospital for Children, Tufts University School of Medicine, Boston, MA, USA
    Methods Mol Biol 444:303-9. 2008
    ..Furthermore, the improvements allow for exploration of widely unknown genetic, pathophysiological, and kinetic issues of cell-free fetal DNA in AF...
  42. ncbi request reprint Cell-free fetal DNA in the cerebrospinal fluid of women during the peripartum period
    Robert M Angert
    Division of Genetics Medicine, Department of Pediatrics, Tufts New England Medical Center, Boston, MA 02111, USA
    Am J Obstet Gynecol 190:1087-90. 2004
    ..The purpose of this study was to determine whether cell-free fetal DNA is detectable in the cerebrospinal fluid of women during pregnancy and after delivery...
  43. pmc Murine maternal cell microchimerism: analysis using real-time PCR and in vivo imaging
    Eric C Su
    Division of Newborn Medicine, Department of Pediatrics, Floating Hospital for Children, Tufts New England Medical Center, Boston, MA 02111, USA
    Biol Reprod 78:883-7. 2008
    ..Pregnancy history appears to play a role in maternal cell trafficking. The role of MCM in pregnancy and disease pathogenesis remains to be elucidated...
  44. ncbi request reprint A simple and sensitive erythroblast scoring system to identify fetal cells in maternal blood
    Donghyun Cha
    Division of Genetics, Departments of Obstetrics and Gynecology and Pediatrics, Tufts New England Medical Center, Boston, MA 02111, USA
    Prenat Diagn 23:68-73. 2003
    ....
  45. ncbi request reprint Fetal cell-free DNA circulates in the plasma of pregnant mice: relevance for animal models of fetomaternal trafficking
    Kiarash Khosrotehrani
    Division of Genetics, Departments of Pediatrics and Obstetrics and Gynecology, Tufts New England Medical Center, Boston, MA, USA
    Hum Reprod 19:2460-4. 2004
    ..Our aim was to determine if fDNA could be detected in maternal plasma during pregnancy in a mouse model system. We then compared the levels of fDNA during pregnancies in which the mother and fetus were either congenic or allogenic...
  46. ncbi request reprint Fetomaternal cell traffic, pregnancy-associated progenitor cells, and autoimmune disease
    Diana W Bianchi
    Division of Genetics, Departments of Pediatrics, Obstetrics and Gynecology, Tufts New England Medical Center, Tufts University School of Medicine, Boston, MA 02111, USA
    Best Pract Res Clin Obstet Gynaecol 18:959-75. 2004
    ..Recently developed animal models will help to ascertain whether these cells cause disease, respond to disease, or have therapeutic applications...
  47. ncbi request reprint Presence of filterable and nonfilterable cell-free mRNA in amniotic fluid
    Paige B Larrabee
    Division of Newborn Medicine, Department of Pediatrics, Floating Hospital for Children, Tufts New England Medical Center, Boston, MA 02111, USA
    Clin Chem 51:1024-6. 2005
  48. ncbi request reprint Plasma gamma-globin gene expression suggests that fetal hematopoietic cells contribute to the pool of circulating cell-free fetal nucleic acids during pregnancy
    Tuangsit Wataganara
    Division of Genetics, Department of Pediatrics, Tufts New England Medical Center, Boston, MA 02111, USA
    Clin Chem 50:689-93. 2004
    ..We developed an assay for gamma-globin mRNA concentrations to determine whether hematopoietic cells also contribute to the pool of fetal mRNA in maternal plasma...
  49. pmc Transcriptomic analysis of cell-free fetal RNA suggests a specific molecular phenotype in trisomy 18
    Keiko Koide
    Division of Genetics, Department of Pediatrics, Floating Hospital for Children at Tufts Medical Center, 800 Washington Street, Box 394, Boston, MA 02111, USA
    Hum Genet 129:295-305. 2011
    ..This study contributes novel information regarding functional developmental gene expression differences in fetuses with trisomy 18...
  50. ncbi request reprint Spot counting to locate fetal cells in maternal blood and tissue: a comparison of manual and automated microscopy
    Kirby L Johnson
    Division of Genetics, Department of Pediatrics, Tufts New England Medical Center, Boston, Massachusetts 02111, USA
    Microsc Res Tech 70:585-8. 2007
    ..Our objective was to compare manual scoring to a commercially available automated scanning system for the detection of chromosome signals by fluorescence in situ hybridization (FISH)...
  51. ncbi request reprint Placental volume, as measured by 3-dimensional sonography and levels of maternal plasma cell-free fetal DNA
    Tuangsit Wataganara
    Division of Genetics, Department of Pediatrics, Tufts New England Medical Center, Boston, MA 02111, USA
    Am J Obstet Gynecol 193:496-500. 2005
    ..We tested the hypothesis that if the placenta is the major contributor of cff DNA, then an increased placental volume should be associated with higher maternal plasma cff DNA levels...
  52. ncbi request reprint Inverse correlation between maternal weight and second trimester circulating cell-free fetal DNA levels
    Tuangsit Wataganara
    Division of Genetics, Department of Pediatrics, Institute of Clinical Research and Health Policy Studies, Tufts New England Medical Center, Boston, Massachusetts 02111, USA
    Obstet Gynecol 104:545-50. 2004
    ..We conducted this study to estimate the effect of selected demographic factors on fetal DNA levels in the first and second trimesters of pregnancy...
  53. doi request reprint Tracking fetal development through molecular analysis of maternal biofluids
    Andrea G Edlow
    Mother Infant Research Institute, Tufts Medical Center, Boston, MA 02111, USA
    Biochim Biophys Acta 1822:1970-80. 2012
    ..This article is part of a Special Issue entitled: Molecular Genetics of Human Reproductive Failure...
  54. doi request reprint Massively parallel sequencing of maternal plasma DNA in 113 cases of fetal nuchal cystic hygroma
    Diana W Bianchi
    Mother Infant Research Institute at Tufts Medical Center and Tufts University School of Medicine, Boston, Massachusetts, USA
    Obstet Gynecol 121:1057-62. 2013
    ..To estimate the accuracy and potential clinical effect of using massively parallel sequencing of maternal plasma DNA to detect fetal aneuploidy in a cohort of pregnant women carrying fetuses with nuchal cystic hygroma...
  55. pmc Neonatal salivary analysis reveals global developmental gene expression changes in the premature infant
    Jill L Maron
    Floating Hospital for Children at Tufts Medical Center, Department of Pediatrics, Division of Newborn Medicine, 800 Washington St, Box 44, Boston, MA 02111, USA
    Clin Chem 56:409-16. 2010
    ..There is an important need to develop noninvasive biomarkers to detect disease in premature neonates. Our objective was to determine if salivary genomic analysis provides novel information about neonatal expression of developmental genes...
  56. ncbi request reprint Fetal cells in maternal tissue following pregnancy: what are the consequences?
    Kirby L Johnson
    Division of Genetics, Department of Pediatrics, Tufts New England Medical Center, Box 394, 750 Washington Street Boston, MA 02111, USA
    Hum Reprod Update 10:497-502. 2004
    ....
  57. ncbi request reprint Natural history of fetal cell microchimerism during and following murine pregnancy
    Kiarash Khosrotehrani
    Division of Genetics, Department of Obstetrics and Gynecology, Tufts New England Medical Center, 750 Washington Street, P O Box 394, Boston, MA 02111, USA
    J Reprod Immunol 66:1-12. 2005
    ..Using sensitive methods of detection, we demonstrate that fetal cell microchimerism occurs during all murine pregnancies. We describe a useful model for the study of the consequences of this phenomenon...
  58. pmc pH but not hypoxia affects neonatal gene expression: relevance for housekeeping gene selection
    Jill L Maron
    Department of Pediatrics, Tufts Medical Center, Boston, MA 02111, USA
    J Matern Fetal Neonatal Med 21:443-7. 2008
    ..To identify a candidate neonatal housekeeping gene and to determine the effects of pH and PaO(2) on the stability of newborn gene expression in physiologically hypoxic and acidotic newborn blood...
  59. pmc Maternal obesity affects fetal neurodevelopmental and metabolic gene expression: a pilot study
    Andrea G Edlow
    Mother Infant Research Institute, Tufts Medical Center, Boston, Massachusetts, United States of America
    PLoS ONE 9:e88661. 2014
    ..Underlying molecular mechanisms are poorly understood. We performed a global gene expression analysis of mid-trimester amniotic fluid cell-free fetal RNA in obese versus lean pregnant women...
  60. pmc Neuropeptide Y2 receptor (NPY2R) expression in saliva predicts feeding immaturity in the premature neonate
    Jill L Maron
    Division of Newborn Medicine, Department of Pediatrics, Floating Hospital for Children at Tufts Medical Center, Boston, Massachusetts, United States of America
    PLoS ONE 7:e37870. 2012
    ....
  61. pmc Fetal cell microchimerism and cancer: a nexus of reproduction, immunology, and tumor biology
    Lisa R Kallenbach
    Division of Hematology and Oncology, Department of Medicine, Tufts Medical Center, Boston, Massachusetts 02111, USA
    Cancer Res 71:8-12. 2011
    ..This research will require further study using a multidisciplinary approach, incorporating knowledge of the fetomaternal relationship, tumor biology, immunology, and clinical oncology...
  62. doi request reprint Use of array comparative genomic hybridization for prenatal diagnosis of fetuses with sonographic anomalies and normal metaphase karyotype
    Linda Kleeman
    Division of Maternal Fetal Medicine, Tufts Medical Center, Boston, MA, USA
    Prenat Diagn 29:1213-7. 2009
    ..To prospectively study the addition of array comparative genomic hybridization (CGH) to the prenatal evaluation of fetal structural anomalies...
  63. ncbi request reprint Maternal serum cell-free fetal DNA levels are increased in cases of trisomy 13 but not trisomy 18
    Tuangsit Wataganara
    Department of Pediatrics, Obstetrics and Gynecology, Division of Genetics, Tufts New England Medical Center, Tufts University School of Medicine, 750 Washington Street, Box 394, Boston, MA 02111, USA
    Hum Genet 112:204-8. 2003
    ..Fetal DNA may facilitate noninvasive screening for trisomy 13 provided that a gender-independent fetal DNA marker can be developed...
  64. ncbi request reprint Combined FISH and immunolabeling on paraffin-embedded tissue sections for the study of microchimerism
    Kiarash Khosrotehrani
    Tufts New England Medical Center, 750 Washington Street, Boston, MA 02111, USA
    Biotechniques 34:242-4. 2003
  65. ncbi request reprint The natural history of trisomy 12p
    Reeval Segel
    Department of Pediatrics, Division of Genetics, Tufts New England Medical Center, Boston, Massachusetts 02111, USA
    Am J Med Genet A 140:695-703. 2006
    ..We also provide recommendations for the long-term follow-up of patients with trisomy 12p...
  66. pmc Maternal background strain influences fetal-maternal trafficking more than maternal immune competence in mice
    Lisa R Kallenbach
    Division of Hematology and Oncology, Department of Medicine, Tufts Medical Center, Boston, MA 02111, USA
    J Reprod Immunol 90:188-94. 2011
    ..Since allogenic matings are more similar to those that occur in humans, future studies using animal models of microchimerism should consider incorporating this type of experimental design...
  67. pmc Trophoblastic oxidative stress and the release of cell-free feto-placental DNA
    May Lee Tjoa
    Department of Pediatrics, Division of Genetics, Tufts New England Medical Center, Boston, Massachusetts, USA
    Am J Pathol 169:400-4. 2006
    ....
  68. doi request reprint Genome-wide fetal aneuploidy detection by maternal plasma DNA sequencing
    Diana W Bianchi
    Mother Infant Research Institute, Tufts Medical Center and Tufts University School of Medicine, Boston, Massachusetts, USA
    Obstet Gynecol 119:890-901. 2012
    ..To prospectively determine the diagnostic accuracy of massively parallel sequencing to detect whole chromosome fetal aneuploidy from maternal plasma...
  69. ncbi request reprint Fetal cell microchimerism: helpful or harmful to the parous woman?
    Kiarash Khosrotehrani
    Division of Genetics, Department of Pediatrics, Obstetrics and Gynecology, Tufts New England Medical Center and Tufts University School of Medicine, Boston, Massachusetts 02111, USA
    Curr Opin Obstet Gynecol 15:195-9. 2003
    ..In this review we will summarize more recent data that provide a new insight into bi-directional feto-maternal cell trafficking...
  70. ncbi request reprint Sharpening the tools: a summary of a National Institutes of Health workshop on new technologies for detection of fetal cells in maternal blood for early prenatal diagnosis
    Diana W Bianchi
    Departments of Pediatrics and Obstetrics and Gynecology, Tufts New England Medical Center and Tufts University School of Medicine, 750 Washington Street, Boston, MA 02111, USA
    J Matern Fetal Neonatal Med 19:199-207. 2006
    ..The passage of time since the workshop has allowed an objective assessment of where the research has progressed. A 2006 update on the field is included at the end of the summary...
  71. pmc Cord blood genomic analysis highlights the role of redox balance
    Jill L Maron
    Division of Newborn Medicine, Department of Pediatrics, Floating Hospital for Children at Tufts Medical Center, Boston, MA 02111, USA
    Free Radic Biol Med 49:992-6. 2010
    ..Understanding the genes necessary to maintain this delicate redox balance may lead to the development of alternative treatment strategies...
  72. pmc Microarray analysis of cell-free fetal DNA in amniotic fluid: a prenatal molecular karyotype
    Paige B Larrabee
    Division of Newborn Medicine, Department of Pediatrics, Tufts New England Medical Center, Tufts University School of Medicine, Boston, MA 02111, USA
    Am J Hum Genet 75:485-91. 2004
    ..This technology facilitates rapid screening of samples for whole-chromosome changes and may augment standard karyotyping techniques by providing additional molecular information...
  73. pmc High-throughput discovery and characterization of fetal protein trafficking in the blood of pregnant women
    Jill L Maron
    Floating Hospital for Children at Tufts Medical Center, Department of Pediatrics, Division of Newborn Medicine 800 Washington Street, Box 44 Boston, MA 02111
    Proteomics Clin Appl 3:1389-96. 2009
    ..This study raises important questions regarding the biological effects of fetal proteins on the pregnant woman...
  74. ncbi request reprint Persistent elevation of cell-free fetal DNA levels in maternal plasma after selective laser coagulation of chorionic plate anastomoses in severe midgestational twin-twin transfusion syndrome
    Tuangsit Wataganara
    Division of Genetics, Department of Pediatrics, Obstetrics and Gynecology, Tufts New England Medical Center, Tufts University School of Medicine, Boston, Mass, USA
    Am J Obstet Gynecol 192:604-9. 2005
    ..This study was undertaken to determine whether laser thermocoagulation for twin-twin transfusion syndrome (TTTS) causes increased cell-free fetal DNA levels in maternal plasma, potentially as a result of placental injury...
  75. ncbi request reprint Interlaboratory comparison of fetal male DNA detection from common maternal plasma samples by real-time PCR
    Kirby L Johnson
    Division of Genetics, Department of Pediatrics, Tufts New England Medical Center, Boston, MA 02111, USA
    Clin Chem 50:516-21. 2004
    ..To further evaluate this potential, we developed and validated a standard protocol to determine whether fetal DNA sequences could be reproducibly amplified and measured across multiple laboratories in a common set of specimens...
  76. doi request reprint Prenatal pharmacotherapy for fetal anomalies: a 2011 update
    Lisa Hui
    Mother Infant Research Institute, Tufts Medical Center, Boston, MA, USA
    Prenat Diagn 31:735-43. 2011
    ..To date, the majority of pharmacotherapies for fetal anomalies have been evaluated only in retrospective, uncontrolled studies. The way forward will be with an evidence-based approach to prenatal pharmacological interventions...
  77. doi request reprint Genetic considerations in the prenatal diagnosis of overgrowth syndromes
    Neeta Vora
    Division of Genetics, Department of Pediatrics, Department of Obstetrics, Floating Hospital for Children and Tufts Medical Center, Boston, MA 02111, USA
    Prenat Diagn 29:923-9. 2009
    ..Here, we review prenatal sonographic findings in fetal overgrowth syndromes, including Pallister-Killian, Beckwith-Wiedemann, Sotos, Perlman, and Simpson-Golabi-Behmel. We also discuss prenatal diagnosis options and recurrence risks...
  78. ncbi request reprint Fetal cell isolation from maternal blood cultures by flow cytometric hemoglobin profiles. Results of a preliminary clinical trial
    Ralph M Bohmer
    Division of Genetics, Department of Pediatrics, New England Medical Center and Tufts University Medical School, Boston, MA 02111, USA
    Fetal Diagn Ther 17:83-9. 2002
    ..We conducted a trial to test if the blood of pregnant women contains fetal clonogenic erythroid cells the progeny of which can be identified and isolated by a newly developed flow-sorting procedure...
  79. ncbi request reprint Fetal diaphragmatic hernia and upper limb anomalies suggest Brachmann-de Lange syndrome
    Teresa Marino
    Division of Maternal Fetal Medicine, Department of Pediatrics, New England Medical Center and Tufts University School of Medicine, Boston, MA 02111, USA
    Prenat Diagn 22:144-7. 2002
    ..The prenatal diagnosis of diaphragmatic hernia with associated anomalies should prompt consideration of an underlying genetic etiology...
  80. doi request reprint Microchimerism in endocrine pathology
    Daniel W Rust
    Department of Pathology, Tufts Medical Center, Boston, MA, USA
    Endocr Pathol 20:11-6. 2009
    ..Studies of materno-fetal microchimerism have documented the presence of maternal cells in juvenile diabetes and other pediatric conditions. Microchimerism plays a potential role in the repair of diseased thyroid and pancreatic tissues...
  81. doi request reprint Reproductive issues for adults with autosomal dominant polycystic kidney disease
    Neeta Vora
    Department of Pediatrics, Tufts New England Medical Center and Floating Hospital for Children, Boston, MA 02111, USA
    Am J Kidney Dis 51:307-18. 2008
    ..We review in detail these topics to familiarize physicians taking care of patients with ADPKD with the reproductive issues that confront affected individuals...
  82. ncbi request reprint Fetomaternal cell trafficking and the stem cell debate: gender matters
    Diana W Bianchi
    Division of Genetics, Department of Pediatrics, Tufts New England Medical Center and Tufts University School of Medicine, Boston, Mass 02111, USA
    JAMA 297:1489-91. 2007
  83. ncbi request reprint Maternal cell microchimerism in newborn tissues
    Bharath Srivatsa
    Division of Genetics, Department of Pediatrics, Floating Hospital for Children at the New England Medical Center, Tufts University School of Medicine, Boston, Massachusetts, USA
    J Pediatr 142:31-5. 2003
    ..On the basis of reports of maternal cells being detected in the umbilical cord blood of newborn infants, we tested the hypothesis that maternal cells can migrate out of the circulation into newborn tissues...
  84. ncbi request reprint Fetal cell-free plasma DNA concentrations in maternal blood are stable 24 hours after collection: analysis of first- and third-trimester samples
    Robert M Angert
    Division of Genetics, Department of Pediatrics, Tufts New England Medical Center and Tufts University School of Medicine, Boston, MA 02111, USA
    Clin Chem 49:195-8. 2003
  85. ncbi request reprint Noninvasive prenatal diagnosis of fetal Rhesus D: ready for Prime(r) Time
    Diana W Bianchi
    Division of Genetics, Department of Pediatrics, Tufts New England Medical Center, Boston, Massachusetts, 02111, USA
    Obstet Gynecol 106:841-4. 2005
    ..The United States should begin to undertake clinical trials to bring this technology to patient care as soon as possible...
  86. ncbi request reprint Fetal therapy for single gene disorders
    Barbara O'Brien
    Division of Genetics, Department of Pediatrics, Tufts New England Medical Center, Boston, Massachusetts 02111, USA
    Clin Obstet Gynecol 48:885-96. 2005