IRVING M contact SHAPIRO

Summary

Affiliation: Thomas Jefferson University
Country: USA

Publications

  1. pmc Metabolic consideration of epiphyseal growth: survival responses in a taxing environment
    Irving M Shapiro
    Department of Orthopaedic Surgery, Thomas Jefferson University Philadelphia, Philadelphia, PA 19107, USA
    Bone 40:561-7. 2007
  2. pmc An organ culture system to model early degenerative changes of the intervertebral disc
    Ravi K Ponnappan
    Department of Orthopaedic Surgery, Thomas Jefferson University, 1015 Walnut Street, Philadelphia, PA 19107, USA
    Arthritis Res Ther 13:R171. 2011
  3. ncbi request reprint Molecular engineering of an orthopaedic implant: from bench to bedside
    I M Shapiro
    Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, PA, USA
    Eur Cell Mater 23:362-70. 2012
  4. pmc Is the spinal motion segment a diarthrodial polyaxial joint: what a nice nucleus like you doing in a joint like this?
    Irving M Shapiro
    Department of Orthopaedic Surgery, Division of Orthopaedic Research, Jefferson Medical College, Philadelphia, PA 19107, USA
    Bone 50:771-6. 2012
  5. ncbi request reprint Fate of the hypertrophic chondrocyte: microenvironmental perspectives on apoptosis and survival in the epiphyseal growth plate
    Irving M Shapiro
    Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    Birth Defects Res C Embryo Today 75:330-9. 2005
  6. ncbi request reprint Fibroblast growth factor-2 maintains the differentiation potential of nucleus pulposus cells in vitro: implications for cell-based transplantation therapy
    Tsung Ting Tsai
    Department of Orthopaedic Surgery and Graduate Program in Tissue Engineering and Regenerative Medicine, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Spine (Phila Pa 1976) 32:495-502. 2007
  7. ncbi request reprint Expression of acid-sensing ion channel 3 (ASIC3) in nucleus pulposus cells of the intervertebral disc is regulated by p75NTR and ERK signaling
    Yoshiyasu Uchiyama
    Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, PA 19107, USA
    J Bone Miner Res 22:1996-2006. 2007
  8. ncbi request reprint The fate of the terminally differentiated chondrocyte: evidence for microenvironmental regulation of chondrocyte apoptosis
    Christopher S Adams
    Department of Orthopaedic Surgery, Thomas Jefferson Medical College, 1015 Walnut Street, 501, Philadelphia, PA 19107, USA
    Crit Rev Oral Biol Med 13:465-73. 2002
  9. pmc Chondrocyte autophagy is stimulated by HIF-1 dependent AMPK activation and mTOR suppression
    Jolene Bohensky
    Department of Orthopedic Surgery, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Pediatr Nephrol 25:633-42. 2010
  10. ncbi request reprint Covalently attached vancomycin provides a nanoscale antibacterial surface
    Valentin Antoci
    Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Clin Orthop Relat Res 461:81-7. 2007

Research Grants

Collaborators

Detail Information

Publications74

  1. pmc Metabolic consideration of epiphyseal growth: survival responses in a taxing environment
    Irving M Shapiro
    Department of Orthopaedic Surgery, Thomas Jefferson University Philadelphia, Philadelphia, PA 19107, USA
    Bone 40:561-7. 2007
    ..The role of HIF, PHDs and other proteins concerned with transduction of the oxemic response is considered and related to chondrocyte survival in a complex extracellular matrix...
  2. pmc An organ culture system to model early degenerative changes of the intervertebral disc
    Ravi K Ponnappan
    Department of Orthopaedic Surgery, Thomas Jefferson University, 1015 Walnut Street, Philadelphia, PA 19107, USA
    Arthritis Res Ther 13:R171. 2011
    ..This approach is based on our previous studies that indicate that organ culture closely models molecular events that occur in vivo in an ex vivo setting...
  3. ncbi request reprint Molecular engineering of an orthopaedic implant: from bench to bedside
    I M Shapiro
    Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, PA, USA
    Eur Cell Mater 23:362-70. 2012
    ..From all of these perspectives, the tethered antibiotic holds promise of providing a novel and practical approach to reducing implant-associated infections...
  4. pmc Is the spinal motion segment a diarthrodial polyaxial joint: what a nice nucleus like you doing in a joint like this?
    Irving M Shapiro
    Department of Orthopaedic Surgery, Division of Orthopaedic Research, Jefferson Medical College, Philadelphia, PA 19107, USA
    Bone 50:771-6. 2012
    ..Hopefully, reclassification will encourage further analysis of the structure and function of the two types of overlapping joints and provide common new insights into diseases that afflict the many joints of the human skeleton...
  5. ncbi request reprint Fate of the hypertrophic chondrocyte: microenvironmental perspectives on apoptosis and survival in the epiphyseal growth plate
    Irving M Shapiro
    Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    Birth Defects Res C Embryo Today 75:330-9. 2005
    ..These two concepts (type II programmed cell death and ischemia reperfusion injury) emphasize the importance of the local microenvironment, in particular pO(2), in directing chondrocyte survival and apoptosis...
  6. ncbi request reprint Fibroblast growth factor-2 maintains the differentiation potential of nucleus pulposus cells in vitro: implications for cell-based transplantation therapy
    Tsung Ting Tsai
    Department of Orthopaedic Surgery and Graduate Program in Tissue Engineering and Regenerative Medicine, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Spine (Phila Pa 1976) 32:495-502. 2007
    ..To investigate effects of FGF-2 on nucleus pulposus cell growth and differentiation...
  7. ncbi request reprint Expression of acid-sensing ion channel 3 (ASIC3) in nucleus pulposus cells of the intervertebral disc is regulated by p75NTR and ERK signaling
    Yoshiyasu Uchiyama
    Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, PA 19107, USA
    J Bone Miner Res 22:1996-2006. 2007
    ..These findings suggest that ASIC3 may adapt disc cells to their hydrodynamically stressed microenvironment...
  8. ncbi request reprint The fate of the terminally differentiated chondrocyte: evidence for microenvironmental regulation of chondrocyte apoptosis
    Christopher S Adams
    Department of Orthopaedic Surgery, Thomas Jefferson Medical College, 1015 Walnut Street, 501, Philadelphia, PA 19107, USA
    Crit Rev Oral Biol Med 13:465-73. 2002
    ..It is concluded that specific microenvironments exist in cartilage that can serve to direct chondrocyte apoptosis...
  9. pmc Chondrocyte autophagy is stimulated by HIF-1 dependent AMPK activation and mTOR suppression
    Jolene Bohensky
    Department of Orthopedic Surgery, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Pediatr Nephrol 25:633-42. 2010
    ..Based on these findings, it is concluded that because of the micro-environmental changes experienced by the chondrocyte, autophagy is activated by AMPK in a HIF-1-dependent manner...
  10. ncbi request reprint Covalently attached vancomycin provides a nanoscale antibacterial surface
    Valentin Antoci
    Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Clin Orthop Relat Res 461:81-7. 2007
    ..Based on these observations, covalent modification of the titanium surface with an antibiotic may be viewed as a potential new tool in preventing or eliminating periprosthetic infection...
  11. ncbi request reprint Vancomycin bound to Ti rods reduces periprosthetic infection: preliminary study
    Valentin Antoci
    Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, PA, USA
    Clin Orthop Relat Res 461:88-95. 2007
    ..Vancomycin-modified implants showed superior inhibition of bacterial attachment and proliferation compared to control titanium surfaces...
  12. ncbi request reprint Galectin-3 expression in the intervertebral disc: a useful marker of the notochord phenotype?
    Erbil Oguz
    Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Spine (Phila Pa 1976) 32:9-16. 2007
    ..Galectin-3 expression in rat intervertebral disc at different stages in postnatal life is evaluated...
  13. ncbi request reprint Nucleus pulposus cells express HIF-1 alpha under normoxic culture conditions: a metabolic adaptation to the intervertebral disc microenvironment
    Makarand V Risbud
    Department of Orthopaedic Surgery and Graduate Program in Tissue Engineering and Regenerative Medicine, Thomas Jefferson University, Philadelphia, Pennsylvania, 19107
    J Cell Biochem 98:152-9. 2006
    ..The study confirmed that HIF-1alpha can be used as a phenotypic marker of nucleus pulposus cells...
  14. pmc PI3K/AKT regulates aggrecan gene expression by modulating Sox9 expression and activity in nucleus pulposus cells of the intervertebral disc
    Chin Chang Cheng
    Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    J Cell Physiol 221:668-76. 2009
    ..Results of these studies demonstrate for the first time that PI3K/AKT signaling controls aggrecan gene expression, in part by modulating Sox9 expression and activity in cells of the nucleus pulposus...
  15. doi request reprint Controlled release of vancomycin from thin sol-gel films on implant surfaces successfully controls osteomyelitis
    Christopher S Adams
    Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, Pennsylvania, USA
    J Orthop Res 27:701-9. 2009
    ..These results clearly demonstrate that this novel thin sol-gel technology can be used for the targeted delivery of antibiotics for the treatment of periprosthetic as well as other bone infections...
  16. doi request reprint Cited2 modulates hypoxia-inducible factor-dependent expression of vascular endothelial growth factor in nucleus pulposus cells of the rat intervertebral disc
    Amit Agrawal
    Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    Arthritis Rheum 58:3798-808. 2008
    ....
  17. ncbi request reprint Evidence for skeletal progenitor cells in the degenerate human intervertebral disc
    Makarand V Risbud
    Department of Orthopaedic Surgery and Graduate Program in Tissue Engineering and Regenerative Medicine, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Spine (Phila Pa 1976) 32:2537-44. 2007
    ..To identify and characterize endogenous progenitor cell population from intervertebral disc...
  18. doi request reprint Micro-CT analysis with multiple thresholds allows detection of bone formation and resorption during ultrasound-treated fracture healing
    Theresa A Freeman
    Department of Orthopedic Surgery, Thomas Jefferson University, Curtis Building, Suite 501, 1015 Walnut Street, Philadelphia, Pennsylvania 19107 5099, USA
    J Orthop Res 27:673-9. 2009
    ..Results of the study indicate that at sites of bone turnover, this multithresholding analytical technique can be used to provide quantitative information on bone formation, as well as resorption...
  19. doi request reprint Akt-1 mediates survival of chondrocytes from endoplasmic reticulum-induced stress
    Jeremy Price
    Department of Pathology, University of Pennsylvania School of Dental Medicine, Philadelphia, Pennsylvania 19104, USA
    J Cell Physiol 222:502-8. 2010
    ..Thus, rapid upregulation of CHOP likely contributes to chondrocyte death, while Akt-1-mediated inactivation of caspase 3 and induction of BclXL promotes survival...
  20. ncbi request reprint Antibiotics for local delivery systems cause skeletal cell toxicity in vitro
    Valentin Antoci
    Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, PA, USA
    Clin Orthop Relat Res 462:200-6. 2007
    ..Released lactate dehydrogenase ratios were severely increased in osteoblasts. These data suggest the balance between the targeted microbicidal effects and host cellular toxicity is critical for skeletal cell survival and function...
  21. ncbi request reprint PIM-2 is an independent regulator of chondrocyte survival and autophagy in the epiphyseal growth plate
    Jolene Bohensky
    Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    J Cell Physiol 213:246-51. 2007
    ..We conclude that PIM-2 regulates a new intermediate stage in the differentiation pathway, the induction of autophagy...
  22. ncbi request reprint HIF-1 regulation of chondrocyte apoptosis: induction of the autophagic pathway
    Jolene Bohensky
    Department of Orthopedic Surgery, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    Autophagy 3:207-14. 2007
    ..This study indicates that HIF-1 serves to regulate both autophagy and apoptosis...
  23. ncbi request reprint HIF-1 alpha is a regulator of galectin-3 expression in the intervertebral disc
    Yan Zeng
    Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, PA, USA
    J Bone Miner Res 22:1851-61. 2007
    ..Our studies indicate that HIF-1 alpha regulates galectin-3 expression by interacting with hypoxia regulatory elements in the promoter region. Finally, we show that galectin-3 serves a prosurvival role in the intervertebral disc...
  24. pmc Hypoxic induction of UCP3 in the growth plate: UCP3 suppresses chondrocyte autophagy
    Hitoshi Watanabe
    Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    J Cell Physiol 216:419-25. 2008
    ..In addition, it inhibits induction of the autophagic response. When this occurs, it suppresses sensitivity to agents that promote chondrocyte deletion from the growth plate...
  25. pmc Oxygen tension regulates preosteocyte maturation and mineralization
    Adam M Zahm
    Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Bone 43:25-31. 2008
    ..Based on these findings, it is concluded that the osteogenic activity of preosteocyte-like cells is dependent on both the O2 tension and the expression of HIF-1alpha...
  26. ncbi request reprint Chondrocytes embedded in the epiphyseal growth plates of long bones undergo autophagy prior to the induction of osteogenesis
    Vickram Srinivas
    Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    Autophagy 2:215-6. 2006
    ..Whether chondrocytes can recover from this state, and possibly participate in osteogenesis, is not known at this time...
  27. pmc Toward an understanding of the role of notochordal cells in the adult intervertebral disc: from discord to accord
    Makarand V Risbud
    Department of Orthopedic Surgery and Graduate Program in Tissue Engineering and Regenerative Medicine, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    Dev Dyn 239:2141-8. 2010
    ..Thus, the most critical choice for a suitable animal model should relate more to the anatomical and mechanical characteristics of the motion segment than concerns of cell loss and replacement by non-notochordal cells...
  28. ncbi request reprint An organ culture system for the study of the nucleus pulposus: description of the system and evaluation of the cells
    Makarand V Risbud
    Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    Spine (Phila Pa 1976) 28:2652-8; discussion 2658-9. 2003
    ..The goal of this study was to develop a methodology to maintain intervertebral discs in organ culture, thereby preserving tissue architecture and metabolic function in a three-dimensional environment...
  29. ncbi request reprint Decellularized vein as a potential scaffold for vascular tissue engineering
    Patrick J Schaner
    Department of Surgery, Thomas Jefferson University, Philadelphia, PA, USA
    J Vasc Surg 40:146-53. 2004
    ..Our purpose was to study the composition and strength of decellularized vein to determine its potential as a vascular tissue-engineering scaffold...
  30. pmc Hypoxic regulation of nucleus pulposus cell survival: from niche to notch
    Makarand V Risbud
    Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Am J Pathol 176:1577-83. 2010
    ..We predict that knowledge of each of these interactive proteins within the disk niche could be used to enhance renewal and promote differentiation and function of cells of the nucleus pulposus...
  31. ncbi request reprint Vancomycin covalently bonded to titanium alloy prevents bacterial colonization
    Valentin Antoci
    Department of Orthopaedic Surgery, Thomas Jefferson University, 1015 Walnut Street, Suite 501, Philadelphia, Pennsylvania 19107, USA
    J Orthop Res 25:858-66. 2007
    ..These results demonstrate that we have effectively engineered a stable, bactericidal Ti surface. This new surface holds great promise in terms of mitigating or preventing periprosthetic infection...
  32. ncbi request reprint Differentiation of mesenchymal stem cells towards a nucleus pulposus-like phenotype in vitro: implications for cell-based transplantation therapy
    Makarand V Risbud
    Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    Spine (Phila Pa 1976) 29:2627-32. 2004
    ..Because mesenchymal stem cells can differentiate into chondrocyte-like cells, we ask the question, can mesenchymal stem cells commit to the nucleus pulposus phenotype?..
  33. pmc Reversine enhances generation of progenitor-like cells by dedifferentiation of annulus fibrosus cells
    Mansi Saraiya
    Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, PA, USA
    Tissue Eng Part A 16:1443-55. 2010
    ..This agent could be used to generate skeletal progenitor cells to orchestrate the repair of the intervertebral disc...
  34. ncbi request reprint Nucleus pulposus cells upregulate PI3K/Akt and MEK/ERK signaling pathways under hypoxic conditions and resist apoptosis induced by serum withdrawal
    Makarand V Risbud
    Division of Orthopaedic Research, Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Spine (Phila Pa 1976) 30:882-9. 2005
    ..To examine the impact of hypoxia, rat nucleus pulposus cells were maintained in monolayer culture in 2% O2 and survival and signal transduction pathways identified...
  35. ncbi request reprint TonEBP/OREBP is a regulator of nucleus pulposus cell function and survival in the intervertebral disc
    Tsung Ting Tsai
    Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    J Biol Chem 281:25416-24. 2006
    ..It is concluded that TonEBP permits the disc cells to adapt to the hyperosmotic milieu while autoregulating the expression of molecules that generate the unique extracellular environment...
  36. ncbi request reprint Toward an optimum system for intervertebral disc organ culture: TGF-beta 3 enhances nucleus pulposus and anulus fibrosus survival and function through modulation of TGF-beta-R expression and ERK signaling
    Makarand V Risbud
    Department of Orthopaedic Surgery and Graduate Program in Tissue Engineering and Regenerative Medicine, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Spine (Phila Pa 1976) 31:884-90. 2006
    ..Rat lumbar discs comprising nucleus pulposus, annulus fibrosus, and cartilaginous endplates were cultured for 1 week in a specialized media containing either TGF-beta1 or TGF-beta3. Role of TGF-beta isoforms on cell function was evaluated...
  37. pmc A novel short hairpin RNA (shRNA) expression system promotes Sox9-dependent gene silencing
    James R Gilbert
    Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Plasmid 62:50-5. 2009
    ..Its applications for both basic mechanistic studies and therapeutic purposes should facilitate the design and implementation of innovative tissue engineering strategies...
  38. ncbi request reprint Osteogenic potential of adult human stem cells of the lumbar vertebral body and the iliac crest
    Makarand V Risbud
    Department of Orthopaedic Surgery and Graduate Program in Tissue Engineering and Regenerative Medicine, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Spine (Phila Pa 1976) 31:83-9. 2006
    ..Progenitor cells were isolated using standard culture conditions and their osteogenic potential evaluated...
  39. pmc Topographic features retained after antibiotic modification of Ti alloy surfaces: retention of topography with attachment of antibiotics
    Constantinos Ketonis
    Department of Orthopedic Surgery, Thomas Jefferson University, 1015 Walnut Street, Suite 501, Philadelphia, PA 19107, USA
    Clin Orthop Relat Res 467:1678-87. 2009
    ..Using this technique, we believe it is possible to passivate many complex implant designs/geometries as a first step toward covalent bonding of antibiotics or other bioactive factors...
  40. ncbi request reprint Hypoxia activates MAPK activity in rat nucleus pulposus cells: regulation of integrin expression and cell survival
    Makarand V Risbud
    Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Spine (Phila Pa 1976) 30:2503-9. 2005
    ..The aim of the present study was to investigate whether activation of MAPK subtypes ERK and p38 influences integrin expression and promotes nucleus pulposus cell survival in hypoxia...
  41. pmc Vancomycin bonded to bone grafts prevents bacterial colonization
    Constantinos Ketonis
    Department of Orthopaedic Surgery, Jefferson Medical College, Philadelphia, PA 19107, USA
    Antimicrob Agents Chemother 55:487-94. 2011
    ..Since the covalently tethered antibiotic is stable, is active, retains its specificity, and does not exhibit toxicity, it is concluded that this modified allograft holds great promise for decreasing bone graft-associated infections...
  42. pmc Regulation of CCN2/connective tissue growth factor expression in the nucleus pulposus of the intervertebral disc: role of Smad and activator protein 1 signaling
    Cassie M Tran
    Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    Arthritis Rheum 62:1983-92. 2010
    ..To investigate transforming growth factor beta (TGFbeta) regulation of connective tissue growth factor (CTGF) expression in cells of the nucleus pulposus of rats, mice, and humans...
  43. pmc SMAD3 functions as a transcriptional repressor of acid-sensing ion channel 3 (ASIC3) in nucleus pulposus cells of the intervertebral disc
    Yoshiyasu Uchiyama
    Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    J Bone Miner Res 23:1619-28. 2008
    ..Results of these studies clearly show that TGFbeta is highly expressed in the degenerate disc and through smad3 serves as a negative regulator of ASIC3 expression...
  44. pmc Autophagy in mineralizing tissues: microenvironmental perspectives
    Vickram Srinivas
    Department of Orthopaedic Surgery, Jefferson Medical College of Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    Cell Cycle 8:391-3. 2009
    ..From a clinical viewpoint, it may be possible to enhance skeletal cell survival through drugs that modulate the autophagic state and prevent the induction of apoptosis...
  45. ncbi request reprint MEK/ERK signaling controls osmoregulation of nucleus pulposus cells of the intervertebral disc by transactivation of TonEBP/OREBP
    Tsung Ting Tsai
    Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, Pennsylvania19107, USA
    J Bone Miner Res 22:965-74. 2007
    ..However, the mechanism of hyperosmotic activation of TonEBP in disc cells is not known. Results of this study show that hypertonic activation of ERK signaling regulates transactivation activity of TonEBP, modulating its function...
  46. ncbi request reprint Fibronectin adsorption on surface-activated poly(dimethylsiloxane) and its effect on cellular function
    George K Toworfe
    Center for Bioactive Materials and Tissue Engineering, Department of Bioengineering, University of Pennsylvania School of Engineering and Applied Science, 120 Hayden Hall, 3320 Smith Walk, Philadelphia, Pennsylvania 19104 6392, USA
    J Biomed Mater Res A 71:449-61. 2004
    ..Higher attachment of MC3T3-E1 osteoblast-like cells was observed on PDMS*-Fn than on PDMS-Fn. Moreover, cell spreading and cytoskeleton organization after 72 h was clearly favored on the Fn-coated PDMS* surfaces...
  47. pmc Antibiotic modification of native grafts: improving upon nature's scaffolds
    Constantinos Ketonis
    Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    Tissue Eng Part A 16:2041-9. 2010
    ..We suggest that this chimeric construct represents a new generation of antibiotic-modified allografts that provide antibacterial properties...
  48. pmc Linear shear conditioning improves vascular graft retention of adipose-derived stem cells by upregulation of the alpha5beta1 integrin
    Stephen E McIlhenny
    Department of Surgery, Thomas Jefferson University, Philadelphia, Pennsylvania, USA
    Tissue Eng Part A 16:245-55. 2010
    ..These results indicated a novel method for seeding, conditioning, and retaining of adult stem cells on a decellularized vein scaffold within a high-shear stress microenvironment...
  49. ncbi request reprint Stem cell regeneration of the nucleus pulposus
    Makarand V Risbud
    Department of Orthopaedic Surgery, Thomas Jefferson University, 925 Chestnut Street, Philadelphia, PA 19107, USA
    Spine J 4:348S-353S. 2004
    ..While the work is in its infancy, there is significant optimism that next steps will lead to organ culture models of disc degeneration and regeneration, and ultimately to in vivo rescue of degenerating discs with stem cell treatment...
  50. ncbi request reprint Apoptosis and survival of osteoblast-like cells are regulated by surface attachment
    Vavara Grigoriou
    Department of Orthodontics, School of Dental Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Biol Chem 280:1733-9. 2005
    ..These results indicated that the binding of cells to RGDS abrogated apoptosis via the mitochondrial pathway and that the suppression of apoptosis was dependent on the activity of phosphatidylinositol 3-kinase...
  51. pmc Hypoxia-inducible factor regulation of ANK expression in nucleus pulposus cells: possible implications in controlling dystrophic mineralization in the intervertebral disc
    Renata Skubutyte
    Jefferson Medical College, Philadelphia, Pennsylvania, USA
    Arthritis Rheum 62:2707-15. 2010
    ..Since nucleus pulposus cells reside under conditions of hypoxia, we determined if the expression of ANK, a pyrophosphate transporter, is regulated by the hypoxia-inducible factor (HIF) proteins...
  52. ncbi request reprint Phenotypic characteristics of the nucleus pulposus: expression of hypoxia inducing factor-1, glucose transporter-1 and MMP-2
    Ramesh Rajpurohit
    Department of Orthopaedic Surgery, Jefferson Medical College, Thomas Jefferson University, 501 Curtis Building, 1015 Walnut St, Philadelphia, PA 19107, USA
    Cell Tissue Res 308:401-7. 2002
    ..Moreover, the presence of these isoforms provides evidence that cells of the disc respond to hypoxia and nutrient stress by upregulating stress-responsive genes...
  53. ncbi request reprint Matrix regulation of skeletal cell apoptosis II: role of Arg-Gly-Asp-containing peptides
    Robert L Perlot
    Department of Orthodontics, School of Dental Medicine, University of Pennsylvania, Philadelphia, USA
    J Bone Miner Res 17:66-76. 2002
    ..Results of the investigation support the view that resorption of the extracellular matrix generates peptide products that can induce apoptosis of vicinal cells...
  54. ncbi request reprint Cell-based therapy for disc repair
    D Greg Anderson
    Graduate Program in Tissue Engineering and Regenerative Medicine and Department of Orthopaedic Surgery, Thomas Jefferson University, 925 Chestnut Street, Philadelphia, PA 19107, USA
    Spine J 5:297S-303S. 2005
    ..One of the most promising therapies for symptomatic disc degeneration involves the implantation of therapeutic cells into the degenerative disc...
  55. pmc Autophagy: a new phase in the maturation of growth plate chondrocytes is regulated by HIF, mTOR and AMP kinase
    Vickram Srinivas
    Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Cells Tissues Organs 189:88-92. 2009
    ..Once autophagy is activated, the postmitotic chondrocytes would be expected to remain viable in their unique microenvironment and complete their life cycle...
  56. ncbi request reprint Normoxic stabilization of HIF-1alpha drives glycolytic metabolism and regulates aggrecan gene expression in nucleus pulposus cells of the rat intervertebral disk
    Amit Agrawal
    Dept of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Am J Physiol Cell Physiol 293:C621-31. 2007
    ..Results of this study indicate that oxygen-independent stabilization of HIF-1alpha in nucleus pulposus cells is a metabolic adaptation that drives glycolysis and aggrecan expression...
  57. ncbi request reprint Covalent bonding of vancomycin to Ti6Al4V alloy pins provides long-term inhibition of Staphylococcus aureus colonization
    Om P Edupuganti
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Bioorg Med Chem Lett 17:2692-6. 2007
    ..These results provide a basis for testing self-protection against S. aureus colonization in animal models...
  58. pmc In situ oxygen utilization in the rat intervertebral disc
    Deanna C Lee
    Department of Orthopedic Surgery, Thomas Jefferson University, Philadelphia, PA 19107, USA
    J Anat 210:294-303. 2007
    ..Accordingly, the cells of the intervertebral disc are not severely hypoxic...
  59. pmc Numerical modeling of oxygen distributions in cortical and cancellous bone: oxygen availability governs osteonal and trabecular dimensions
    Adam M Zahm
    Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, Pennsylvania, USA
    Am J Physiol Cell Physiol 299:C922-9. 2010
    ..Based on these results, it is concluded that significant oxygen gradients exist within cortical and cancellous bone and that the oxygen tension may regulate the physical dimensions of both osteons and bone trabeculae...
  60. ncbi request reprint Detection of apoptotic gene expression in human osteoblast-like cells by cDNA microarrays
    Sleiman Razzouk
    Department of Orthopaedic Surgery, Curtis Bldg, Room 50, Thomas Jefferson University, 1015 Walnut Street, Philadelphia, PA 19107 5099, USA
    J Bone Miner Metab 21:261-7. 2003
    ..Results of this study suggest that the ion pair influenced ERK1 and TNFR1 signaling pathways and affected thiol metabolism, whereas Bcl-2 and Bax were expressed at late stages of the death process...
  61. pmc Activation of TonEBP by calcium controls {beta}1,3-glucuronosyltransferase-I expression, a key regulator of glycosaminoglycan synthesis in cells of the intervertebral disc
    Akihiko Hiyama
    Department of Orthopedic Surgery, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    J Biol Chem 284:9824-34. 2009
    ..The results suggest that by controlling both GAG and aggrecan synthesis, disc cells can autoregulate their osmotic environment and accommodate mechanical loading...
  62. pmc BMP-2 and TGF-beta stimulate expression of beta1,3-glucuronosyl transferase 1 (GlcAT-1) in nucleus pulposus cells through AP1, TonEBP, and Sp1: role of MAPKs
    Akihiko Hiyama
    Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, PA 19107, USA
    J Bone Miner Res 25:1179-90. 2010
    ..It is concluded that by controlling both GAG and aggrecan synthesis, these growth factors positively influence disk cell function...
  63. ncbi request reprint In vivo behavior of decellularized vein allograft
    Niels D Martin
    Department of Surgery, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    J Surg Res 129:17-23. 2005
    ..We are investigating decellularized vein allograft as a scaffold to engineer a non-synthetic, small-diameter vascular graft. This study examines the in vivo behavior of this scaffolding after implantation into the arterial circulation...
  64. ncbi request reprint Intrauterine fetal constraint induces chondrocyte apoptosis and premature ossification of the cranial base
    James M Smartt
    Department of Surgery and Division of Plastic Surgery, The Children s Hospital of Philadelphia, University of Pennsylvania School of Medicine, Philadelphia, PA, USA
    Plast Reconstr Surg 116:1363-9. 2005
    ..The purpose of these experiments was to test the hypothesis that intrauterine head constraint produces ultrastructural changes in the spheno-occipital synchondroses of fetal mice...
  65. ncbi request reprint Mercury-induced apoptosis in human lymphocytes: caspase activation is linked to redox status
    Bruce J Shenker
    Department of Pathology, University of Pennsylvania School of Dental Medicine, Philadelphia, PA 19104 6002, USA
    Antioxid Redox Signal 4:379-89. 2002
    ..Additonally, mercury acts as a genotoxin significantly altering the expression of genes that affect cell survival and apoptosis...
  66. ncbi request reprint Selfprotective smart orthopedic implants
    Javad Parvizi
    Thomas Jefferson University, Rothman Institute of Orthopedics, 925 Chestnut Street, Philadelphia, PA 19107, USA
    Expert Rev Med Devices 4:55-64. 2007
    ..This new technology has the potential to eliminate periprosthetic infection, a major and growing problem in orthopedic practice...
  67. ncbi request reprint Maturation-dependent thiol loss increases chondrocyte susceptibility to apoptosis
    Cristina C Teixeira
    Department of Basic Science and Craniofacial Biology and Department of Orthodontics, New York University, College of Dentistry, New York, New York 10010, USA
    J Bone Miner Res 18:662-8. 2003
    ..It was concluded that the loss of thiol reserve is not only linked to the expression of the hypertrophic phenotype but also influenced chondrocyte survival, linking chondrocyte maturation and the activation of the apoptotic pathway...
  68. ncbi request reprint Nitric oxide-nitric oxide synthase regulates key maturational events during chondrocyte terminal differentiation
    Cristina C Teixeira
    Department of Basic Science and Craniofacial Biology, and Department of Orthodontics, College of Dentistry, New York University, 345 East 24th Street, New York, NY 10010, USA
    Bone 37:37-45. 2005
    ..Since expression of these two determinants was blocked by inhibitors of the cGMP pathway, it is concluded that NO metabolism is required for development of the mature chondrocyte phenotype...
  69. ncbi request reprint The effect of starch and starch-bioactive glass composite microparticles on the adhesion and expression of the osteoblastic phenotype of a bone cell line
    Gabriela A Silva
    3B s Research Group Biomaterials, Biodegradables and Biomimetics University of Minho, Campus de Gualtar, 4710 057 Braga, Portugal
    Biomaterials 28:326-34. 2007
    ..Consequently, they can be used as templates for forming hybrid constructs aiming to be applied in bone-tissue-engineering applications...
  70. ncbi request reprint Development of the terminally differentiated state sensitizes epiphyseal chondrocytes to apoptosis through caspase-3 activation
    Bruna Pucci
    Department of Cellular and Molecular Pathology, IRCCS San Raffaele Pisana, Rome, Italy
    J Cell Physiol 210:609-15. 2007
    ..Only when the terminally differentiated chondrocytes are challenged with low levels of apoptogens there is activation of apoptosis...
  71. ncbi request reprint Matrix regulation of skeletal cell apoptosis III: mechanism of ion pair-induced apoptosis
    Ray Saunders
    Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, PA 19107 5099, USA
    J Cell Biochem 100:703-15. 2007
    ..Together, these data demonstrate that Ca(2+) Pi-induced osteoblast apoptosis is characterized by the generation of an apoptosome and that Ca(2+) release from ER stores may promote ion pair-dependent cell death...
  72. ncbi request reprint Modeling of phosphate ion transfer to the surface of osteoblasts under normal gravity and simulated microgravity conditions
    Karthik Mukundakrishnan
    Department of Mechanical Engineering and Applied Mechanics, School of Engineering and Applied Science, University of Pennsylvania, Philadelphia, PA 19104 6315, USA
    Ann N Y Acad Sci 1027:85-98. 2004
    ..Such increased accumulation may lead to enhanced apoptosis and may help explain the increased bone loss observed under microgravity conditions...
  73. ncbi request reprint Adhesion of MC3T3-E1 cells to RGD peptides of different flanking residues: detachment strength and correlation with long-term cellular function
    Mark H Lee
    Department of Bioengineering, University of Pennsylvania, Philadelphia, PA 19104, USA
    J Biomed Mater Res A 81:150-60. 2007
    ..These findings collectively illustrate both the advantages and limitations of enhancing cellular adhesion and function by the design of RGD peptides...
  74. ncbi request reprint Starch-based microparticles as vehicles for the delivery of active platelet-derived growth factor
    Gabriela A Silva
    3B s Research Group Biomaterials, Biodegradables and Biomimetics, University of Minho, Campus de Gualtar, Braga, Portugal
    Tissue Eng 13:1259-68. 2007
    ..When combined with previous results, these materials also suggest their ability to enhance the regenerating potential of tissue engineering hybrid constructs...

Research Grants31

  1. BONE GROWTH IN DENTAL, CRANIAL AND SKELETAL TISSUES
    Irving Shapiro; Fiscal Year: 2006
    ..abstract_text> ..
  2. Regeneration of Nucleus Pulposus by Tissue Engineering
    Irving Shapiro; Fiscal Year: 2007
    ..In the long term, if successful, this approach to therapy will entail using patients' own cells for disc regeneration. ..
  3. Training in Tissue Engineering and Regenerative Medicine
    Irving Shapiro; Fiscal Year: 2007
    ....
  4. Smart Substrates for a New Generation of Implants
    Irving Shapiro; Fiscal Year: 2009
    ..Outcomes from this study will provide information for engineering of orthopaedic and dental implants that can be tested at the clinical level. ..
  5. Regeneration of Nucleus Pulposus by Tissue Engineering
    Irving Shapiro; Fiscal Year: 2009
    ..The goal of the proposal is to understand the mechanism that controls the fate and function of these progenitor cells in both the normal and diseased intervertebral disc. ..
  6. BONE GROWTH IN DENTAL, CRANIAL AND SKELETAL TISSUES
    Irving Shapiro; Fiscal Year: 2001
    ..The analysis will consist of FACTS flow cytometry based on alkaline phosphatase or annexin content and phospholipid packing. ..
  7. CRANIOFACIAL DEVELOPMENT: METABOLIC CONTROL OF APOPTOSIS
    Irving Shapiro; Fiscal Year: 2003
    ..Taken together, these studies should highlight the molecular basis of bone cell apoptosis, a phenomenon that is now considered critical for bone growth and skeletal remodeling. ..
  8. Regeneration of Nucleus Pulposus by Tissue Engineering
    IRVING M contact SHAPIRO; Fiscal Year: 2010
    ..The goal of the proposal is to understand the mechanism that controls the fate and function of these progenitor cells in both the normal and diseased intervertebral disc. ..