Research Topics
Species | IRVING M contact SHAPIROSummaryAffiliation: Thomas Jefferson University Country: USA Publications
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Publications
Metabolic consideration of epiphyseal growth: survival responses in a taxing environmentIrving M Shapiro
Department of Orthopaedic Surgery, Thomas Jefferson University Philadelphia, Philadelphia, PA 19107, USA
Bone 40:561-7. 2007..The role of HIF, PHDs and other proteins concerned with transduction of the oxemic response is considered and related to chondrocyte survival in a complex extracellular matrix...- An organ culture system to model early degenerative changes of the intervertebral discRavi K Ponnappan
Department of Orthopaedic Surgery, Thomas Jefferson University, 1015 Walnut Street, Philadelphia, PA 19107, USA
Arthritis Res Ther 13:R171. 2011..This approach is based on our previous studies that indicate that organ culture closely models molecular events that occur in vivo in an ex vivo setting... 
Molecular engineering of an orthopaedic implant: from bench to bedsideI M Shapiro
Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, PA, USA
Eur Cell Mater 23:362-70. 2012..From all of these perspectives, the tethered antibiotic holds promise of providing a novel and practical approach to reducing implant-associated infections...- Is the spinal motion segment a diarthrodial polyaxial joint: what a nice nucleus like you doing in a joint like this?Irving M Shapiro
Department of Orthopaedic Surgery, Division of Orthopaedic Research, Jefferson Medical College, Philadelphia, PA 19107, USA
Bone 50:771-6. 2012..Hopefully, reclassification will encourage further analysis of the structure and function of the two types of overlapping joints and provide common new insights into diseases that afflict the many joints of the human skeleton... - Fate of the hypertrophic chondrocyte: microenvironmental perspectives on apoptosis and survival in the epiphyseal growth plateIrving M Shapiro
Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
Birth Defects Res C Embryo Today 75:330-9. 2005..These two concepts (type II programmed cell death and ischemia reperfusion injury) emphasize the importance of the local microenvironment, in particular pO(2), in directing chondrocyte survival and apoptosis... - Fibroblast growth factor-2 maintains the differentiation potential of nucleus pulposus cells in vitro: implications for cell-based transplantation therapyTsung Ting Tsai
Department of Orthopaedic Surgery and Graduate Program in Tissue Engineering and Regenerative Medicine, Thomas Jefferson University, Philadelphia, PA 19107, USA
Spine (Phila Pa 1976) 32:495-502. 2007..To investigate effects of FGF-2 on nucleus pulposus cell growth and differentiation... - Expression of acid-sensing ion channel 3 (ASIC3) in nucleus pulposus cells of the intervertebral disc is regulated by p75NTR and ERK signalingYoshiyasu Uchiyama
Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, PA 19107, USA
J Bone Miner Res 22:1996-2006. 2007..These findings suggest that ASIC3 may adapt disc cells to their hydrodynamically stressed microenvironment... - The fate of the terminally differentiated chondrocyte: evidence for microenvironmental regulation of chondrocyte apoptosisChristopher S Adams
Department of Orthopaedic Surgery, Thomas Jefferson Medical College, 1015 Walnut Street, 501, Philadelphia, PA 19107, USA
Crit Rev Oral Biol Med 13:465-73. 2002..It is concluded that specific microenvironments exist in cartilage that can serve to direct chondrocyte apoptosis... - Chondrocyte autophagy is stimulated by HIF-1 dependent AMPK activation and mTOR suppressionJolene Bohensky
Department of Orthopedic Surgery, Thomas Jefferson University, Philadelphia, PA 19107, USA
Pediatr Nephrol 25:633-42. 2010..Based on these findings, it is concluded that because of the micro-environmental changes experienced by the chondrocyte, autophagy is activated by AMPK in a HIF-1-dependent manner... - Covalently attached vancomycin provides a nanoscale antibacterial surfaceValentin Antoci
Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, PA 19107, USA
Clin Orthop Relat Res 461:81-7. 2007..Based on these observations, covalent modification of the titanium surface with an antibiotic may be viewed as a potential new tool in preventing or eliminating periprosthetic infection... - Vancomycin bound to Ti rods reduces periprosthetic infection: preliminary studyValentin Antoci
Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, PA, USA
Clin Orthop Relat Res 461:88-95. 2007..Vancomycin-modified implants showed superior inhibition of bacterial attachment and proliferation compared to control titanium surfaces... - Galectin-3 expression in the intervertebral disc: a useful marker of the notochord phenotype?Erbil Oguz
Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, PA 19107, USA
Spine (Phila Pa 1976) 32:9-16. 2007..Galectin-3 expression in rat intervertebral disc at different stages in postnatal life is evaluated... - Nucleus pulposus cells express HIF-1 alpha under normoxic culture conditions: a metabolic adaptation to the intervertebral disc microenvironmentMakarand V Risbud
Department of Orthopaedic Surgery and Graduate Program in Tissue Engineering and Regenerative Medicine, Thomas Jefferson University, Philadelphia, Pennsylvania, 19107
J Cell Biochem 98:152-9. 2006..The study confirmed that HIF-1alpha can be used as a phenotypic marker of nucleus pulposus cells... - PI3K/AKT regulates aggrecan gene expression by modulating Sox9 expression and activity in nucleus pulposus cells of the intervertebral discChin Chang Cheng
Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
J Cell Physiol 221:668-76. 2009..Results of these studies demonstrate for the first time that PI3K/AKT signaling controls aggrecan gene expression, in part by modulating Sox9 expression and activity in cells of the nucleus pulposus... 
Controlled release of vancomycin from thin sol-gel films on implant surfaces successfully controls osteomyelitisChristopher S Adams
Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, Pennsylvania, USA
J Orthop Res 27:701-9. 2009..These results clearly demonstrate that this novel thin sol-gel technology can be used for the targeted delivery of antibiotics for the treatment of periprosthetic as well as other bone infections...- Cited2 modulates hypoxia-inducible factor-dependent expression of vascular endothelial growth factor in nucleus pulposus cells of the rat intervertebral discAmit Agrawal
Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
Arthritis Rheum 58:3798-808. 2008.... - Evidence for skeletal progenitor cells in the degenerate human intervertebral discMakarand V Risbud
Department of Orthopaedic Surgery and Graduate Program in Tissue Engineering and Regenerative Medicine, Thomas Jefferson University, Philadelphia, PA 19107, USA
Spine (Phila Pa 1976) 32:2537-44. 2007..To identify and characterize endogenous progenitor cell population from intervertebral disc... - Micro-CT analysis with multiple thresholds allows detection of bone formation and resorption during ultrasound-treated fracture healingTheresa A Freeman
Department of Orthopedic Surgery, Thomas Jefferson University, Curtis Building, Suite 501, 1015 Walnut Street, Philadelphia, Pennsylvania 19107 5099, USA
J Orthop Res 27:673-9. 2009..Results of the study indicate that at sites of bone turnover, this multithresholding analytical technique can be used to provide quantitative information on bone formation, as well as resorption... - Akt-1 mediates survival of chondrocytes from endoplasmic reticulum-induced stressJeremy Price
Department of Pathology, University of Pennsylvania School of Dental Medicine, Philadelphia, Pennsylvania 19104, USA
J Cell Physiol 222:502-8. 2010..Thus, rapid upregulation of CHOP likely contributes to chondrocyte death, while Akt-1-mediated inactivation of caspase 3 and induction of BclXL promotes survival... - Antibiotics for local delivery systems cause skeletal cell toxicity in vitroValentin Antoci
Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, PA, USA
Clin Orthop Relat Res 462:200-6. 2007..Released lactate dehydrogenase ratios were severely increased in osteoblasts. These data suggest the balance between the targeted microbicidal effects and host cellular toxicity is critical for skeletal cell survival and function... - PIM-2 is an independent regulator of chondrocyte survival and autophagy in the epiphyseal growth plateJolene Bohensky
Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
J Cell Physiol 213:246-51. 2007..We conclude that PIM-2 regulates a new intermediate stage in the differentiation pathway, the induction of autophagy... - HIF-1 regulation of chondrocyte apoptosis: induction of the autophagic pathwayJolene Bohensky
Department of Orthopedic Surgery, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
Autophagy 3:207-14. 2007..This study indicates that HIF-1 serves to regulate both autophagy and apoptosis... - HIF-1 alpha is a regulator of galectin-3 expression in the intervertebral discYan Zeng
Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, PA, USA
J Bone Miner Res 22:1851-61. 2007..Our studies indicate that HIF-1 alpha regulates galectin-3 expression by interacting with hypoxia regulatory elements in the promoter region. Finally, we show that galectin-3 serves a prosurvival role in the intervertebral disc... - Hypoxic induction of UCP3 in the growth plate: UCP3 suppresses chondrocyte autophagyHitoshi Watanabe
Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
J Cell Physiol 216:419-25. 2008..In addition, it inhibits induction of the autophagic response. When this occurs, it suppresses sensitivity to agents that promote chondrocyte deletion from the growth plate... - Oxygen tension regulates preosteocyte maturation and mineralizationAdam M Zahm
Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, PA 19107, USA
Bone 43:25-31. 2008..Based on these findings, it is concluded that the osteogenic activity of preosteocyte-like cells is dependent on both the O2 tension and the expression of HIF-1alpha... - Chondrocytes embedded in the epiphyseal growth plates of long bones undergo autophagy prior to the induction of osteogenesisVickram Srinivas
Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
Autophagy 2:215-6. 2006..Whether chondrocytes can recover from this state, and possibly participate in osteogenesis, is not known at this time... - Toward an understanding of the role of notochordal cells in the adult intervertebral disc: from discord to accordMakarand V Risbud
Department of Orthopedic Surgery and Graduate Program in Tissue Engineering and Regenerative Medicine, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
Dev Dyn 239:2141-8. 2010..Thus, the most critical choice for a suitable animal model should relate more to the anatomical and mechanical characteristics of the motion segment than concerns of cell loss and replacement by non-notochordal cells... - An organ culture system for the study of the nucleus pulposus: description of the system and evaluation of the cellsMakarand V Risbud
Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
Spine (Phila Pa 1976) 28:2652-8; discussion 2658-9. 2003..The goal of this study was to develop a methodology to maintain intervertebral discs in organ culture, thereby preserving tissue architecture and metabolic function in a three-dimensional environment... - Decellularized vein as a potential scaffold for vascular tissue engineeringPatrick J Schaner
Department of Surgery, Thomas Jefferson University, Philadelphia, PA, USA
J Vasc Surg 40:146-53. 2004..Our purpose was to study the composition and strength of decellularized vein to determine its potential as a vascular tissue-engineering scaffold... - Hypoxic regulation of nucleus pulposus cell survival: from niche to notchMakarand V Risbud
Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, PA 19107, USA
Am J Pathol 176:1577-83. 2010..We predict that knowledge of each of these interactive proteins within the disk niche could be used to enhance renewal and promote differentiation and function of cells of the nucleus pulposus... - Vancomycin covalently bonded to titanium alloy prevents bacterial colonizationValentin Antoci
Department of Orthopaedic Surgery, Thomas Jefferson University, 1015 Walnut Street, Suite 501, Philadelphia, Pennsylvania 19107, USA
J Orthop Res 25:858-66. 2007..These results demonstrate that we have effectively engineered a stable, bactericidal Ti surface. This new surface holds great promise in terms of mitigating or preventing periprosthetic infection... - Differentiation of mesenchymal stem cells towards a nucleus pulposus-like phenotype in vitro: implications for cell-based transplantation therapyMakarand V Risbud
Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
Spine (Phila Pa 1976) 29:2627-32. 2004..Because mesenchymal stem cells can differentiate into chondrocyte-like cells, we ask the question, can mesenchymal stem cells commit to the nucleus pulposus phenotype?.. - Reversine enhances generation of progenitor-like cells by dedifferentiation of annulus fibrosus cellsMansi Saraiya
Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, PA, USA
Tissue Eng Part A 16:1443-55. 2010..This agent could be used to generate skeletal progenitor cells to orchestrate the repair of the intervertebral disc... - Nucleus pulposus cells upregulate PI3K/Akt and MEK/ERK signaling pathways under hypoxic conditions and resist apoptosis induced by serum withdrawalMakarand V Risbud
Division of Orthopaedic Research, Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, PA 19107, USA
Spine (Phila Pa 1976) 30:882-9. 2005..To examine the impact of hypoxia, rat nucleus pulposus cells were maintained in monolayer culture in 2% O2 and survival and signal transduction pathways identified... - TonEBP/OREBP is a regulator of nucleus pulposus cell function and survival in the intervertebral discTsung Ting Tsai
Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
J Biol Chem 281:25416-24. 2006..It is concluded that TonEBP permits the disc cells to adapt to the hyperosmotic milieu while autoregulating the expression of molecules that generate the unique extracellular environment... - Toward an optimum system for intervertebral disc organ culture: TGF-beta 3 enhances nucleus pulposus and anulus fibrosus survival and function through modulation of TGF-beta-R expression and ERK signalingMakarand V Risbud
Department of Orthopaedic Surgery and Graduate Program in Tissue Engineering and Regenerative Medicine, Thomas Jefferson University, Philadelphia, PA 19107, USA
Spine (Phila Pa 1976) 31:884-90. 2006..Rat lumbar discs comprising nucleus pulposus, annulus fibrosus, and cartilaginous endplates were cultured for 1 week in a specialized media containing either TGF-beta1 or TGF-beta3. Role of TGF-beta isoforms on cell function was evaluated... - A novel short hairpin RNA (shRNA) expression system promotes Sox9-dependent gene silencingJames R Gilbert
Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, PA 19107, USA
Plasmid 62:50-5. 2009..Its applications for both basic mechanistic studies and therapeutic purposes should facilitate the design and implementation of innovative tissue engineering strategies... - Osteogenic potential of adult human stem cells of the lumbar vertebral body and the iliac crestMakarand V Risbud
Department of Orthopaedic Surgery and Graduate Program in Tissue Engineering and Regenerative Medicine, Thomas Jefferson University, Philadelphia, PA 19107, USA
Spine (Phila Pa 1976) 31:83-9. 2006..Progenitor cells were isolated using standard culture conditions and their osteogenic potential evaluated... - Topographic features retained after antibiotic modification of Ti alloy surfaces: retention of topography with attachment of antibioticsConstantinos Ketonis
Department of Orthopedic Surgery, Thomas Jefferson University, 1015 Walnut Street, Suite 501, Philadelphia, PA 19107, USA
Clin Orthop Relat Res 467:1678-87. 2009..Using this technique, we believe it is possible to passivate many complex implant designs/geometries as a first step toward covalent bonding of antibiotics or other bioactive factors... - Hypoxia activates MAPK activity in rat nucleus pulposus cells: regulation of integrin expression and cell survivalMakarand V Risbud
Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, PA 19107, USA
Spine (Phila Pa 1976) 30:2503-9. 2005..The aim of the present study was to investigate whether activation of MAPK subtypes ERK and p38 influences integrin expression and promotes nucleus pulposus cell survival in hypoxia... - Vancomycin bonded to bone grafts prevents bacterial colonizationConstantinos Ketonis
Department of Orthopaedic Surgery, Jefferson Medical College, Philadelphia, PA 19107, USA
Antimicrob Agents Chemother 55:487-94. 2011..Since the covalently tethered antibiotic is stable, is active, retains its specificity, and does not exhibit toxicity, it is concluded that this modified allograft holds great promise for decreasing bone graft-associated infections... - Regulation of CCN2/connective tissue growth factor expression in the nucleus pulposus of the intervertebral disc: role of Smad and activator protein 1 signalingCassie M Tran
Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
Arthritis Rheum 62:1983-92. 2010..To investigate transforming growth factor beta (TGFbeta) regulation of connective tissue growth factor (CTGF) expression in cells of the nucleus pulposus of rats, mice, and humans... - SMAD3 functions as a transcriptional repressor of acid-sensing ion channel 3 (ASIC3) in nucleus pulposus cells of the intervertebral discYoshiyasu Uchiyama
Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
J Bone Miner Res 23:1619-28. 2008..Results of these studies clearly show that TGFbeta is highly expressed in the degenerate disc and through smad3 serves as a negative regulator of ASIC3 expression... - Autophagy in mineralizing tissues: microenvironmental perspectivesVickram Srinivas
Department of Orthopaedic Surgery, Jefferson Medical College of Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
Cell Cycle 8:391-3. 2009..From a clinical viewpoint, it may be possible to enhance skeletal cell survival through drugs that modulate the autophagic state and prevent the induction of apoptosis... - MEK/ERK signaling controls osmoregulation of nucleus pulposus cells of the intervertebral disc by transactivation of TonEBP/OREBPTsung Ting Tsai
Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, Pennsylvania19107, USA
J Bone Miner Res 22:965-74. 2007..However, the mechanism of hyperosmotic activation of TonEBP in disc cells is not known. Results of this study show that hypertonic activation of ERK signaling regulates transactivation activity of TonEBP, modulating its function... - Fibronectin adsorption on surface-activated poly(dimethylsiloxane) and its effect on cellular functionGeorge K Toworfe
Center for Bioactive Materials and Tissue Engineering, Department of Bioengineering, University of Pennsylvania School of Engineering and Applied Science, 120 Hayden Hall, 3320 Smith Walk, Philadelphia, Pennsylvania 19104 6392, USA
J Biomed Mater Res A 71:449-61. 2004..Higher attachment of MC3T3-E1 osteoblast-like cells was observed on PDMS*-Fn than on PDMS-Fn. Moreover, cell spreading and cytoskeleton organization after 72 h was clearly favored on the Fn-coated PDMS* surfaces... - Antibiotic modification of native grafts: improving upon nature's scaffoldsConstantinos Ketonis
Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
Tissue Eng Part A 16:2041-9. 2010..We suggest that this chimeric construct represents a new generation of antibiotic-modified allografts that provide antibacterial properties... - Linear shear conditioning improves vascular graft retention of adipose-derived stem cells by upregulation of the alpha5beta1 integrinStephen E McIlhenny
Department of Surgery, Thomas Jefferson University, Philadelphia, Pennsylvania, USA
Tissue Eng Part A 16:245-55. 2010..These results indicated a novel method for seeding, conditioning, and retaining of adult stem cells on a decellularized vein scaffold within a high-shear stress microenvironment... - Stem cell regeneration of the nucleus pulposusMakarand V Risbud
Department of Orthopaedic Surgery, Thomas Jefferson University, 925 Chestnut Street, Philadelphia, PA 19107, USA
Spine J 4:348S-353S. 2004..While the work is in its infancy, there is significant optimism that next steps will lead to organ culture models of disc degeneration and regeneration, and ultimately to in vivo rescue of degenerating discs with stem cell treatment... - Apoptosis and survival of osteoblast-like cells are regulated by surface attachmentVavara Grigoriou
Department of Orthodontics, School of Dental Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
J Biol Chem 280:1733-9. 2005..These results indicated that the binding of cells to RGDS abrogated apoptosis via the mitochondrial pathway and that the suppression of apoptosis was dependent on the activity of phosphatidylinositol 3-kinase... - Hypoxia-inducible factor regulation of ANK expression in nucleus pulposus cells: possible implications in controlling dystrophic mineralization in the intervertebral discRenata Skubutyte
Jefferson Medical College, Philadelphia, Pennsylvania, USA
Arthritis Rheum 62:2707-15. 2010..Since nucleus pulposus cells reside under conditions of hypoxia, we determined if the expression of ANK, a pyrophosphate transporter, is regulated by the hypoxia-inducible factor (HIF) proteins... - Phenotypic characteristics of the nucleus pulposus: expression of hypoxia inducing factor-1, glucose transporter-1 and MMP-2Ramesh Rajpurohit
Department of Orthopaedic Surgery, Jefferson Medical College, Thomas Jefferson University, 501 Curtis Building, 1015 Walnut St, Philadelphia, PA 19107, USA
Cell Tissue Res 308:401-7. 2002..Moreover, the presence of these isoforms provides evidence that cells of the disc respond to hypoxia and nutrient stress by upregulating stress-responsive genes... - Matrix regulation of skeletal cell apoptosis II: role of Arg-Gly-Asp-containing peptidesRobert L Perlot
Department of Orthodontics, School of Dental Medicine, University of Pennsylvania, Philadelphia, USA
J Bone Miner Res 17:66-76. 2002..Results of the investigation support the view that resorption of the extracellular matrix generates peptide products that can induce apoptosis of vicinal cells... - Cell-based therapy for disc repairD Greg Anderson
Graduate Program in Tissue Engineering and Regenerative Medicine and Department of Orthopaedic Surgery, Thomas Jefferson University, 925 Chestnut Street, Philadelphia, PA 19107, USA
Spine J 5:297S-303S. 2005..One of the most promising therapies for symptomatic disc degeneration involves the implantation of therapeutic cells into the degenerative disc... - Autophagy: a new phase in the maturation of growth plate chondrocytes is regulated by HIF, mTOR and AMP kinaseVickram Srinivas
Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, PA 19107, USA
Cells Tissues Organs 189:88-92. 2009..Once autophagy is activated, the postmitotic chondrocytes would be expected to remain viable in their unique microenvironment and complete their life cycle... - Normoxic stabilization of HIF-1alpha drives glycolytic metabolism and regulates aggrecan gene expression in nucleus pulposus cells of the rat intervertebral diskAmit Agrawal
Dept of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, PA 19107, USA
Am J Physiol Cell Physiol 293:C621-31. 2007..Results of this study indicate that oxygen-independent stabilization of HIF-1alpha in nucleus pulposus cells is a metabolic adaptation that drives glycolysis and aggrecan expression... - Covalent bonding of vancomycin to Ti6Al4V alloy pins provides long-term inhibition of Staphylococcus aureus colonizationOm P Edupuganti
Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, PA 19107, USA
Bioorg Med Chem Lett 17:2692-6. 2007..These results provide a basis for testing self-protection against S. aureus colonization in animal models... - In situ oxygen utilization in the rat intervertebral discDeanna C Lee
Department of Orthopedic Surgery, Thomas Jefferson University, Philadelphia, PA 19107, USA
J Anat 210:294-303. 2007..Accordingly, the cells of the intervertebral disc are not severely hypoxic... - Numerical modeling of oxygen distributions in cortical and cancellous bone: oxygen availability governs osteonal and trabecular dimensionsAdam M Zahm
Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, Pennsylvania, USA
Am J Physiol Cell Physiol 299:C922-9. 2010..Based on these results, it is concluded that significant oxygen gradients exist within cortical and cancellous bone and that the oxygen tension may regulate the physical dimensions of both osteons and bone trabeculae... - Detection of apoptotic gene expression in human osteoblast-like cells by cDNA microarraysSleiman Razzouk
Department of Orthopaedic Surgery, Curtis Bldg, Room 50, Thomas Jefferson University, 1015 Walnut Street, Philadelphia, PA 19107-5099, USA
J Bone Miner Metab 21:261-7. 2003..Results of this study suggest that the ion pair influenced ERK1 and TNFR1 signaling pathways and affected thiol metabolism, whereas Bcl-2 and Bax were expressed at late stages of the death process... - Activation of TonEBP by calcium controls {beta}1,3-glucuronosyltransferase-I expression, a key regulator of glycosaminoglycan synthesis in cells of the intervertebral discAkihiko Hiyama
Department of Orthopedic Surgery, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
J Biol Chem 284:9824-34. 2009..The results suggest that by controlling both GAG and aggrecan synthesis, disc cells can autoregulate their osmotic environment and accommodate mechanical loading... - BMP-2 and TGF-beta stimulate expression of beta1,3-glucuronosyl transferase 1 (GlcAT-1) in nucleus pulposus cells through AP1, TonEBP, and Sp1: role of MAPKsAkihiko Hiyama
Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, PA 19107, USA
J Bone Miner Res 25:1179-90. 2010..It is concluded that by controlling both GAG and aggrecan synthesis, these growth factors positively influence disk cell function... - In vivo behavior of decellularized vein allograftNiels D Martin
Department of Surgery, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
J Surg Res 129:17-23. 2005..These characteristics make it satisfactory for further tissue engineering; combined with luminal vascular cell seeding, it may prove useful as a small-diameter arterial bypass graft... - Intrauterine fetal constraint induces chondrocyte apoptosis and premature ossification of the cranial baseJames M Smartt
Department of Surgery and Division of Plastic Surgery, The Children's Hospital of Philadelphia, University of Pennsylvania School of Medicine, Philadelphia, PA, USA
Plast Reconstr Surg 116:1363-9. 2005..CONCLUSION: These experiments confirm the ability of intrauterine constraint to induce changes in the morphology and cell biology of the cranial base in synostotic fetuses... - Mercury-induced apoptosis in human lymphocytes: caspase activation is linked to redox statusBruce J Shenker
Department of Pathology, University of Pennsylvania School of Dental Medicine, Philadelphia, PA 19104 6002, USA
Antioxid Redox Signal 4:379-89. 2002..Additonally, mercury acts as a genotoxin significantly altering the expression of genes that affect cell survival and apoptosis... - Selfprotective smart orthopedic implantsJavad Parvizi
Thomas Jefferson University, Rothman Institute of Orthopedics, 925 Chestnut Street, Philadelphia, PA 19107, USA
Expert Rev Med Devices 4:55-64. 2007..This new technology has the potential to eliminate periprosthetic infection, a major and growing problem in orthopedic practice... - Maturation-dependent thiol loss increases chondrocyte susceptibility to apoptosisCristina C Teixeira
Department of Basic Science and Craniofacial Biology and Department of Orthodontics, New York University, College of Dentistry, New York, New York 10010, USA
J Bone Miner Res 18:662-8. 2003..It was concluded that the loss of thiol reserve is not only linked to the expression of the hypertrophic phenotype but also influenced chondrocyte survival, linking chondrocyte maturation and the activation of the apoptotic pathway... - Nitric oxide-nitric oxide synthase regulates key maturational events during chondrocyte terminal differentiationCristina C Teixeira
Department of Basic Science and Craniofacial Biology, and Department of Orthodontics, College of Dentistry, New York University, 345 East 24th Street, New York, NY 10010, USA
Bone 37:37-45. 2005..Since expression of these two determinants was blocked by inhibitors of the cGMP pathway, it is concluded that NO metabolism is required for development of the mature chondrocyte phenotype... - The effect of starch and starch-bioactive glass composite microparticles on the adhesion and expression of the osteoblastic phenotype of a bone cell lineGabriela A Silva
3B s Research Group Biomaterials, Biodegradables and Biomimetics University of Minho, Campus de Gualtar, 4710 057 Braga, Portugal
Biomaterials 28:326-34. 2007..Consequently, they can be used as templates for forming hybrid constructs aiming to be applied in bone-tissue-engineering applications... - Development of the terminally differentiated state sensitizes epiphyseal chondrocytes to apoptosis through caspase-3 activationBruna Pucci
Department of Cellular and Molecular Pathology, IRCCS San Raffaele Pisana, Rome, Italy
J Cell Physiol 210:609-15. 2007..Only when the terminally differentiated chondrocytes are challenged with low levels of apoptogens there is activation of apoptosis... - Matrix regulation of skeletal cell apoptosis III: mechanism of ion pair-induced apoptosisRay Saunders
Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, PA 19107 5099, USA
J Cell Biochem 100:703-15. 2007..Together, these data demonstrate that Ca(2+) Pi-induced osteoblast apoptosis is characterized by the generation of an apoptosome and that Ca(2+) release from ER stores may promote ion pair-dependent cell death... - Modeling of phosphate ion transfer to the surface of osteoblasts under normal gravity and simulated microgravity conditionsKarthik Mukundakrishnan
Thomas Jefferson U, Philadelphia, PA
Ann N Y Acad Sci 1027:85-98. 2004..Such increased accumulation may lead to enhanced apoptosis and may help explain the increased bone loss observed under microgravity conditions... - Adhesion of MC3T3-E1 cells to RGD peptides of different flanking residues: detachment strength and correlation with long-term cellular functionMark H Lee
Department of Bioengineering, University of Pennsylvania, Philadelphia, PA 19104, USA
J Biomed Mater Res A 81:150-60. 2007..These findings collectively illustrate both the advantages and limitations of enhancing cellular adhesion and function by the design of RGD peptides... - Starch-based microparticles as vehicles for the delivery of active platelet-derived growth factorGabriela A Silva
3B s Research Group Biomaterials, Biodegradables and Biomimetics, University of Minho, Campus de Gualtar, Braga, Portugal
Tissue Eng 13:1259-68. 2007..When combined with previous results, these materials also suggest their ability to enhance the regenerating potential of tissue engineering hybrid constructs...
Research Grants
- BONE GROWTH IN DENTAL, CRANIAL AND SKELETAL TISSUESIrving Shapiro; Fiscal Year: 2006..abstract_text> ..
- Regeneration of Nucleus Pulposus by Tissue EngineeringIrving Shapiro; Fiscal Year: 2007..In the long term, if successful, this approach to therapy will entail using patients' own cells for disc regeneration. ..
- Training in Tissue Engineering and Regenerative MedicineIrving Shapiro; Fiscal Year: 2007....
- Smart Substrates for a New Generation of ImplantsIrving Shapiro; Fiscal Year: 2009..Outcomes from this study will provide information for engineering of orthopaedic and dental implants that can be tested at the clinical level. ..
- Regeneration of Nucleus Pulposus by Tissue EngineeringIrving Shapiro; Fiscal Year: 2009..The goal of the proposal is to understand the mechanism that controls the fate and function of these progenitor cells in both the normal and diseased intervertebral disc. ..
- BONE GROWTH IN DENTAL, CRANIAL AND SKELETAL TISSUESIrving Shapiro; Fiscal Year: 2001..The analysis will consist of FACTS flow cytometry based on alkaline phosphatase or annexin content and phospholipid packing. ..
- CRANIOFACIAL DEVELOPMENT: METABOLIC CONTROL OF APOPTOSISIrving Shapiro; Fiscal Year: 2003..Taken together, these studies should highlight the molecular basis of bone cell apoptosis, a phenomenon that is now considered critical for bone growth and skeletal remodeling. ..
- Regeneration of Nucleus Pulposus by Tissue EngineeringIRVING M contact SHAPIRO; Fiscal Year: 2010..The goal of the proposal is to understand the mechanism that controls the fate and function of these progenitor cells in both the normal and diseased intervertebral disc. ..