Makarand Risbud

Summary

Affiliation: Thomas Jefferson University
Country: USA

Publications

  1. ncbi request reprint Stem cells in craniofacial and dental tissue engineering
    M V Risbud
    Graduate Program in Tissue Engineering and Regenerative Medicine, Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Orthod Craniofac Res 8:54-9. 2005
  2. pmc An organ culture system to model early degenerative changes of the intervertebral disc
    Ravi K Ponnappan
    Department of Orthopaedic Surgery, Thomas Jefferson University, 1015 Walnut Street, Philadelphia, PA 19107, USA
    Arthritis Res Ther 13:R171. 2011
  3. ncbi request reprint Nucleus pulposus cells upregulate PI3K/Akt and MEK/ERK signaling pathways under hypoxic conditions and resist apoptosis induced by serum withdrawal
    Makarand V Risbud
    Division of Orthopaedic Research, Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Spine (Phila Pa 1976) 30:882-9. 2005
  4. ncbi request reprint Hypoxia activates MAPK activity in rat nucleus pulposus cells: regulation of integrin expression and cell survival
    Makarand V Risbud
    Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Spine (Phila Pa 1976) 30:2503-9. 2005
  5. ncbi request reprint Osteogenic potential of adult human stem cells of the lumbar vertebral body and the iliac crest
    Makarand V Risbud
    Department of Orthopaedic Surgery and Graduate Program in Tissue Engineering and Regenerative Medicine, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Spine (Phila Pa 1976) 31:83-9. 2006
  6. ncbi request reprint In vivo biocompatibility evaluation of cellulose macrocapsules for islet immunoisolation: Implications of low molecular weight cut-off
    Makarand V Risbud
    Tissue Engineering and Banking Laboratory, National Centre for Cell Science, Ganeshkhind, Pune 411 007, India
    J Biomed Mater Res A 66:86-92. 2003
  7. ncbi request reprint Toward an optimum system for intervertebral disc organ culture: TGF-beta 3 enhances nucleus pulposus and anulus fibrosus survival and function through modulation of TGF-beta-R expression and ERK signaling
    Makarand V Risbud
    Department of Orthopaedic Surgery and Graduate Program in Tissue Engineering and Regenerative Medicine, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Spine (Phila Pa 1976) 31:884-90. 2006
  8. ncbi request reprint Evidence for skeletal progenitor cells in the degenerate human intervertebral disc
    Makarand V Risbud
    Department of Orthopaedic Surgery and Graduate Program in Tissue Engineering and Regenerative Medicine, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Spine (Phila Pa 1976) 32:2537-44. 2007
  9. pmc Hypoxic regulation of nucleus pulposus cell survival: from niche to notch
    Makarand V Risbud
    Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Am J Pathol 176:1577-83. 2010
  10. pmc Toward an understanding of the role of notochordal cells in the adult intervertebral disc: from discord to accord
    Makarand V Risbud
    Department of Orthopedic Surgery and Graduate Program in Tissue Engineering and Regenerative Medicine, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    Dev Dyn 239:2141-8. 2010

Research Grants

  1. Role of HIF-1 in Intervertebral Disc Function
    Makarand Risbud; Fiscal Year: 2009
  2. Role of HIF-1 in Intervertebral Disc Function
    Makarand V Risbud; Fiscal Year: 2010

Collaborators

Detail Information

Publications34

  1. ncbi request reprint Stem cells in craniofacial and dental tissue engineering
    M V Risbud
    Graduate Program in Tissue Engineering and Regenerative Medicine, Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Orthod Craniofac Res 8:54-9. 2005
    ..Increased understanding of the molecular mechanism directing lineage specification and morphogenesis is providing a rational approach for the regeneration of craniofacial tissues and oral structures...
  2. pmc An organ culture system to model early degenerative changes of the intervertebral disc
    Ravi K Ponnappan
    Department of Orthopaedic Surgery, Thomas Jefferson University, 1015 Walnut Street, Philadelphia, PA 19107, USA
    Arthritis Res Ther 13:R171. 2011
    ..This approach is based on our previous studies that indicate that organ culture closely models molecular events that occur in vivo in an ex vivo setting...
  3. ncbi request reprint Nucleus pulposus cells upregulate PI3K/Akt and MEK/ERK signaling pathways under hypoxic conditions and resist apoptosis induced by serum withdrawal
    Makarand V Risbud
    Division of Orthopaedic Research, Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Spine (Phila Pa 1976) 30:882-9. 2005
    ..To examine the impact of hypoxia, rat nucleus pulposus cells were maintained in monolayer culture in 2% O2 and survival and signal transduction pathways identified...
  4. ncbi request reprint Hypoxia activates MAPK activity in rat nucleus pulposus cells: regulation of integrin expression and cell survival
    Makarand V Risbud
    Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Spine (Phila Pa 1976) 30:2503-9. 2005
    ..The aim of the present study was to investigate whether activation of MAPK subtypes ERK and p38 influences integrin expression and promotes nucleus pulposus cell survival in hypoxia...
  5. ncbi request reprint Osteogenic potential of adult human stem cells of the lumbar vertebral body and the iliac crest
    Makarand V Risbud
    Department of Orthopaedic Surgery and Graduate Program in Tissue Engineering and Regenerative Medicine, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Spine (Phila Pa 1976) 31:83-9. 2006
    ..Progenitor cells were isolated using standard culture conditions and their osteogenic potential evaluated...
  6. ncbi request reprint In vivo biocompatibility evaluation of cellulose macrocapsules for islet immunoisolation: Implications of low molecular weight cut-off
    Makarand V Risbud
    Tissue Engineering and Banking Laboratory, National Centre for Cell Science, Ganeshkhind, Pune 411 007, India
    J Biomed Mater Res A 66:86-92. 2003
    ..However, applicability of this membrane is limited due to its low cut-off value. Membranes with a higher molecular weight cut-off may render better glycemic control and higher graft viability...
  7. ncbi request reprint Toward an optimum system for intervertebral disc organ culture: TGF-beta 3 enhances nucleus pulposus and anulus fibrosus survival and function through modulation of TGF-beta-R expression and ERK signaling
    Makarand V Risbud
    Department of Orthopaedic Surgery and Graduate Program in Tissue Engineering and Regenerative Medicine, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Spine (Phila Pa 1976) 31:884-90. 2006
    ..Rat lumbar discs comprising nucleus pulposus, annulus fibrosus, and cartilaginous endplates were cultured for 1 week in a specialized media containing either TGF-beta1 or TGF-beta3. Role of TGF-beta isoforms on cell function was evaluated...
  8. ncbi request reprint Evidence for skeletal progenitor cells in the degenerate human intervertebral disc
    Makarand V Risbud
    Department of Orthopaedic Surgery and Graduate Program in Tissue Engineering and Regenerative Medicine, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Spine (Phila Pa 1976) 32:2537-44. 2007
    ..To identify and characterize endogenous progenitor cell population from intervertebral disc...
  9. pmc Hypoxic regulation of nucleus pulposus cell survival: from niche to notch
    Makarand V Risbud
    Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Am J Pathol 176:1577-83. 2010
    ..We predict that knowledge of each of these interactive proteins within the disk niche could be used to enhance renewal and promote differentiation and function of cells of the nucleus pulposus...
  10. pmc Toward an understanding of the role of notochordal cells in the adult intervertebral disc: from discord to accord
    Makarand V Risbud
    Department of Orthopedic Surgery and Graduate Program in Tissue Engineering and Regenerative Medicine, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    Dev Dyn 239:2141-8. 2010
    ..Thus, the most critical choice for a suitable animal model should relate more to the anatomical and mechanical characteristics of the motion segment than concerns of cell loss and replacement by non-notochordal cells...
  11. pmc Notochordal cells in the adult intervertebral disc: new perspective on an old question
    Makarand V Risbud
    Department of Orthopaedic Surgery and Graduate Program in Tissue Engineering and Regenerative Medicine, Thomas Jefferson University, Philadelphia, PA, USA
    Crit Rev Eukaryot Gene Expr 21:29-41. 2011
    ..In turn, this would lead to decrements in function and enhancement of the effect of agents that are known to promote disc degeneration...
  12. ncbi request reprint Differentiation of mesenchymal stem cells towards a nucleus pulposus-like phenotype in vitro: implications for cell-based transplantation therapy
    Makarand V Risbud
    Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    Spine (Phila Pa 1976) 29:2627-32. 2004
    ..Because mesenchymal stem cells can differentiate into chondrocyte-like cells, we ask the question, can mesenchymal stem cells commit to the nucleus pulposus phenotype?..
  13. ncbi request reprint Cellular therapy for disc degeneration
    David Greg Anderson
    Department of Orthopaedics, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Spine (Phila Pa 1976) 30:S14-9. 2005
    ..Review article regarding the developing field of cellular therapies for symptomatic disc degeneration...
  14. ncbi request reprint Modeling of phosphate ion transfer to the surface of osteoblasts under normal gravity and simulated microgravity conditions
    Karthik Mukundakrishnan
    Department of Mechanical Engineering and Applied Mechanics, School of Engineering and Applied Science, University of Pennsylvania, Philadelphia, PA 19104 6315, USA
    Ann N Y Acad Sci 1027:85-98. 2004
    ..Such increased accumulation may lead to enhanced apoptosis and may help explain the increased bone loss observed under microgravity conditions...
  15. ncbi request reprint Phenotypic characteristics of the nucleus pulposus: expression of hypoxia inducing factor-1, glucose transporter-1 and MMP-2
    Ramesh Rajpurohit
    Department of Orthopaedic Surgery, Jefferson Medical College, Thomas Jefferson University, 501 Curtis Building, 1015 Walnut St, Philadelphia, PA 19107, USA
    Cell Tissue Res 308:401-7. 2002
    ..Moreover, the presence of these isoforms provides evidence that cells of the disc respond to hypoxia and nutrient stress by upregulating stress-responsive genes...
  16. ncbi request reprint Tissue engineering: advances in in vitro cartilage generation
    Makarand V Risbud
    Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, PA, USA
    Trends Biotechnol 20:351-6. 2002
    ..The ability to manipulate and reconstitute tissue structure and function in vitro has tremendous clinical implications and is likely to have a key role in cell and gene therapies in coming years...
  17. ncbi request reprint Expression of acid-sensing ion channel 3 (ASIC3) in nucleus pulposus cells of the intervertebral disc is regulated by p75NTR and ERK signaling
    Yoshiyasu Uchiyama
    Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, PA 19107, USA
    J Bone Miner Res 22:1996-2006. 2007
    ..These findings suggest that ASIC3 may adapt disc cells to their hydrodynamically stressed microenvironment...
  18. ncbi request reprint HIF-1 alpha is a regulator of galectin-3 expression in the intervertebral disc
    Yan Zeng
    Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, PA, USA
    J Bone Miner Res 22:1851-61. 2007
    ..Our studies indicate that HIF-1 alpha regulates galectin-3 expression by interacting with hypoxia regulatory elements in the promoter region. Finally, we show that galectin-3 serves a prosurvival role in the intervertebral disc...
  19. ncbi request reprint Normoxic stabilization of HIF-1alpha drives glycolytic metabolism and regulates aggrecan gene expression in nucleus pulposus cells of the rat intervertebral disk
    Amit Agrawal
    Dept of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Am J Physiol Cell Physiol 293:C621-31. 2007
    ..Results of this study indicate that oxygen-independent stabilization of HIF-1alpha in nucleus pulposus cells is a metabolic adaptation that drives glycolysis and aggrecan expression...
  20. ncbi request reprint MEK/ERK signaling controls osmoregulation of nucleus pulposus cells of the intervertebral disc by transactivation of TonEBP/OREBP
    Tsung Ting Tsai
    Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, Pennsylvania19107, USA
    J Bone Miner Res 22:965-74. 2007
    ..However, the mechanism of hyperosmotic activation of TonEBP in disc cells is not known. Results of this study show that hypertonic activation of ERK signaling regulates transactivation activity of TonEBP, modulating its function...
  21. ncbi request reprint Fibroblast growth factor-2 maintains the differentiation potential of nucleus pulposus cells in vitro: implications for cell-based transplantation therapy
    Tsung Ting Tsai
    Department of Orthopaedic Surgery and Graduate Program in Tissue Engineering and Regenerative Medicine, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Spine (Phila Pa 1976) 32:495-502. 2007
    ..To investigate effects of FGF-2 on nucleus pulposus cell growth and differentiation...
  22. ncbi request reprint Galectin-3 expression in the intervertebral disc: a useful marker of the notochord phenotype?
    Erbil Oguz
    Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Spine (Phila Pa 1976) 32:9-16. 2007
    ..Galectin-3 expression in rat intervertebral disc at different stages in postnatal life is evaluated...
  23. ncbi request reprint TonEBP/OREBP is a regulator of nucleus pulposus cell function and survival in the intervertebral disc
    Tsung Ting Tsai
    Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    J Biol Chem 281:25416-24. 2006
    ..It is concluded that TonEBP permits the disc cells to adapt to the hyperosmotic milieu while autoregulating the expression of molecules that generate the unique extracellular environment...
  24. ncbi request reprint Radio-frequency plasma treatment improves the growth and attachment of endothelial cells on poly(methyl methacrylate) substrates: implications in tissue engineering
    Makarand V Risbud
    Tissue Engineering and Banking Laboratory, National Centre for Cell Science, Ganeshkhind, Pune 411 007, India
    J Biomater Sci Polym Ed 13:1067-80. 2002
    ..Taken collectively, the findings of this study point out the suitability of RF plasma treatment in inducing desirable changes in PMMA substrates, so as to improve their ability to support the growth and attachment of endothelial cells...
  25. ncbi request reprint Stem cell regeneration of the nucleus pulposus
    Makarand V Risbud
    Department of Orthopaedic Surgery, Thomas Jefferson University, 925 Chestnut Street, Philadelphia, PA 19107, USA
    Spine J 4:348S-353S. 2004
    ..While the work is in its infancy, there is significant optimism that next steps will lead to organ culture models of disc degeneration and regeneration, and ultimately to in vivo rescue of degenerating discs with stem cell treatment...
  26. ncbi request reprint Nonporous polyurethane membranes as islet immunoisolation matrices--biocompatibility studies
    Sheela George
    Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram, India
    J Biomater Appl 16:327-40. 2002
    ..Studies indicate that polyurethane membranes T4 and T5 could be potential candidates for islet immunoisolation...
  27. ncbi request reprint Scaffold-based tissue engineering: rationale for computer-aided design and solid free-form fabrication systems
    Dietmar W Hutmacher
    Division of Bioengineering and Department of Orthopaedic Surgery, National University of Singapore, 119260, Singapore
    Trends Biotechnol 22:354-62. 2004
    ..Here, we review the application, advancement and future directions of SFF techniques in the design and creation of scaffolds for use in clinically driven tissue engineering...
  28. ncbi request reprint An organ culture system for the study of the nucleus pulposus: description of the system and evaluation of the cells
    Makarand V Risbud
    Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    Spine (Phila Pa 1976) 28:2652-8; discussion 2658-9. 2003
    ..The goal of this study was to develop a methodology to maintain intervertebral discs in organ culture, thereby preserving tissue architecture and metabolic function in a three-dimensional environment...
  29. ncbi request reprint Hydrogel-coated textile scaffolds as candidate in liver tissue engineering: II. Evaluation of spheroid formation and viability of hepatocytes
    Makarand V Risbud
    Biocompatible Materials Science and Engineering, Swiss Federal Institute of Technology ETH, Zurich, Schlieren, Switzerland
    J Biomater Sci Polym Ed 14:719-31. 2003
    ..These scaffolds could be useful for co-culturing hepatocytes and non-parenchymal endothelial cells in bioartificial liver support systems...
  30. ncbi request reprint Cell-based therapy for disc repair
    D Greg Anderson
    Graduate Program in Tissue Engineering and Regenerative Medicine and Department of Orthopaedic Surgery, Thomas Jefferson University, 925 Chestnut Street, Philadelphia, PA 19107, USA
    Spine J 5:297S-303S. 2005
    ..One of the most promising therapies for symptomatic disc degeneration involves the implantation of therapeutic cells into the degenerative disc...
  31. ncbi request reprint Models of pancreatic regeneration in diabetes
    Makarand V Risbud
    Tissue Engineering and Banking Laboratory, National Centre for Cell Sciences, Ganeshkhind, Pune 411 007, India
    Diabetes Res Clin Pract 58:155-65. 2002
    ....
  32. ncbi request reprint Hydrogel-coated textile scaffolds as three-dimensional growth support for human umbilical vein endothelial cells (HUVECs): possibilities as coculture system in liver tissue engineering
    Makarand V Risbud
    Biocompatible Materials Science and Engineering, Swiss Federal Institute of Technology ETH Zurich
    Cell Transplant 11:369-77. 2002
    ..We foresee the application of this scaffold system in the design of liver bioreactors wherein hepatocytes could be cocultured in parallel with endothelial cells to enhance and preserve liver-specific functions...
  33. ncbi request reprint Current strategies for cell delivery in cartilage and bone regeneration
    Michael Sittinger
    Tissue Engineering Laboratory, Charite University Medicine Berlin, Germany
    Curr Opin Biotechnol 15:411-8. 2004
    ..Thus, to expand the clinical potential, next generation therapies will depend on smart delivery concepts that make use of the regenerative potential of stem cells, morphogenetic growth factors and biomimetic materials...
  34. ncbi request reprint Chitosan: a versatile biopolymer for orthopaedic tissue-engineering
    Alberto Di Martino
    Department of Orthopaedic Surgery and Graduate Program in Tissue Engineering and Regenerative Medicine, Thomas Jefferson University, Philadelphia, PA 19017, USA
    Biomaterials 26:5983-90. 2005
    ..In this paper we will review the current applications and future directions of CS in articular cartilage, intervertebral disk and bone tissue engineering...

Research Grants3

  1. Role of HIF-1 in Intervertebral Disc Function
    Makarand Risbud; Fiscal Year: 2009
    ..The proposed studies will provide insights into mechanisms of early degenerative changes in the disc and may permit development of interventional strategies to prevent degenerative disease. ..
  2. Role of HIF-1 in Intervertebral Disc Function
    Makarand V Risbud; Fiscal Year: 2010
    ..The proposed studies will provide insights into mechanisms of early degenerative changes in the disc and may permit development of interventional strategies to prevent degenerative disease. ..