LORRAINE M IACOVITTI

Summary

Affiliation: Thomas Jefferson University
Country: USA

Publications

  1. pmc A protocol for the differentiation of human embryonic stem cells into dopaminergic neurons using only chemically defined human additives: Studies in vitro and in vivo
    Lorraine Iacovitti
    Farber Institute for Neurosciences, Department of Neurology, Thomas Jefferson University Medical College, Suite 462 Jefferson Hospital for Neuroscience, 900 Walnut Street, Philadelphia, PA 19107, USA
    Brain Res 1127:19-25. 2007
  2. pmc Factors influencing the differentiation of dopaminergic traits in transplanted neural stem cells
    Ming Yang
    Department of Neurology, Thomas Jefferson University Medical College, Philadelphia, Pennsylvania, USA
    Cell Mol Neurobiol 23:851-64. 2003
  3. pmc Adult human bone marrow stromal spheres express neuronal traits in vitro and in a rat model of Parkinson's disease
    Sokreine Suon
    Department of Molecular Cell Biology, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Brain Res 1106:46-51. 2006
  4. ncbi request reprint Purified mouse dopamine neurons thrive and function after transplantation into brain but require novel glial factors for survival in culture
    A E Donaldson
    Farber Institute for Neurosciences, Thomas Jefferson University Medical College, Philadelphia, PA 19107, USA
    Mol Cell Neurosci 30:601-10. 2005
  5. pmc Circumventricular organs: a novel site of neural stem cells in the adult brain
    Lori Bennett
    Farber Institute for Neurosciences, Department of Neurology, Thomas Jefferson University Medical College, 900 Walnut Street, Philadelphia, PA 19107, USA
    Mol Cell Neurosci 41:337-47. 2009
  6. pmc Studies on the differentiation of dopaminergic traits in human neural progenitor cells in vitro and in vivo
    Ming Yang
    Farber Institute for Neurosciences, Department of Neurology, Thomas Jefferson University Medical College, 900 Walnut Street, Philadelphia, PA 19107, USA
    Cell Transplant 13:535-47. 2004
  7. pmc Dopaminergic neurons derived from human induced pluripotent stem cells survive and integrate into 6-OHDA-lesioned rats
    Jingli Cai
    Farber Institute for Neurosciences, Department of Neurology, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Stem Cells Dev 19:1017-23. 2010
  8. pmc Tyrosine hydroxylase gene regulation in human neuronal progenitor cells does not depend on Nurr1 as in the murine and rat systems
    Hao Jin
    The Farber Institute for the Neurosciences, Department of Neurology, Thomas Jefferson University, Jefferson Hospital for the Neurosciences, Philadelphia, PA 19107, USA
    J Cell Physiol 207:49-57. 2006
  9. doi request reprint Human amniotic fluid stem cells do not differentiate into dopamine neurons in vitro or after transplantation in vivo
    Angela E Donaldson
    Department of Neurology, Farber Institute for the Neurosciences, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    Stem Cells Dev 18:1003-12. 2009
  10. ncbi request reprint The human tyrosine hydroxylase gene promoter
    Mark A Kessler
    Department of Neurology and Farber Institute for the Neurosciences, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Brain Res Mol Brain Res 112:8-23. 2003

Research Grants

  1. Neural Stem Cells Grafts in Primate Models of Parkinsons
    Lorraine Iacovitti; Fiscal Year: 2004
  2. Using Stem Cells in Animal Models of Parkinson's Disease
    Lorraine Iacovitti; Fiscal Year: 2006
  3. Imaging stem cell implants in neurodegenerative disease
    Lorraine Iacovitti; Fiscal Year: 2007
  4. STUDIES OF PURIFIED DOPAMINE NEURONS
    Lorraine Iacovitti; Fiscal Year: 2002
  5. FACTORS REGULATING TYROSINE HYDROXYLASE GENE EXPRESSION
    Lorraine Iacovitti; Fiscal Year: 2002
  6. STUDIES OF PURIFIED DOPAMINE NEURONS
    Lorraine Iacovitti; Fiscal Year: 2007

Collaborators

Detail Information

Publications20

  1. pmc A protocol for the differentiation of human embryonic stem cells into dopaminergic neurons using only chemically defined human additives: Studies in vitro and in vivo
    Lorraine Iacovitti
    Farber Institute for Neurosciences, Department of Neurology, Thomas Jefferson University Medical College, Suite 462 Jefferson Hospital for Neuroscience, 900 Walnut Street, Philadelphia, PA 19107, USA
    Brain Res 1127:19-25. 2007
    ..Moreover, transplantation of these cells into the striata of 6-hydroxydopamine-treated rats at the neuronal progenitor stage resulted in the appearance of differentiated DA traits in vivo 2-3 weeks later...
  2. pmc Factors influencing the differentiation of dopaminergic traits in transplanted neural stem cells
    Ming Yang
    Department of Neurology, Thomas Jefferson University Medical College, Philadelphia, Pennsylvania, USA
    Cell Mol Neurobiol 23:851-64. 2003
    ..We conclude that transplanted NSCs can express traits specifically associated with DA neurons but only when cells are grown under certain conditions in vitro and then transplanted in proximity to injury-induced factors present in vivo...
  3. pmc Adult human bone marrow stromal spheres express neuronal traits in vitro and in a rat model of Parkinson's disease
    Sokreine Suon
    Department of Molecular Cell Biology, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Brain Res 1106:46-51. 2006
    ..By 4 weeks, however, all cells had died. Finding ways to prolong survival and promote the appropriate neurotransmitter phenotype is essential if hMSCs are to be clinically useful...
  4. ncbi request reprint Purified mouse dopamine neurons thrive and function after transplantation into brain but require novel glial factors for survival in culture
    A E Donaldson
    Farber Institute for Neurosciences, Thomas Jefferson University Medical College, Philadelphia, PA 19107, USA
    Mol Cell Neurosci 30:601-10. 2005
    ..We conclude that GFP-tagged DA neurons can be purified to homogeneity and can survive and function when grown with glial factors in vitro or after transplantation in vivo...
  5. pmc Circumventricular organs: a novel site of neural stem cells in the adult brain
    Lori Bennett
    Farber Institute for Neurosciences, Department of Neurology, Thomas Jefferson University Medical College, 900 Walnut Street, Philadelphia, PA 19107, USA
    Mol Cell Neurosci 41:337-47. 2009
    ..These findings suggest that CVOs may constitute a heretofore unknown source of stem/progenitor cells, capable of giving rise to new neurons and/or glia in the adult brain...
  6. pmc Studies on the differentiation of dopaminergic traits in human neural progenitor cells in vitro and in vivo
    Ming Yang
    Farber Institute for Neurosciences, Department of Neurology, Thomas Jefferson University Medical College, 900 Walnut Street, Philadelphia, PA 19107, USA
    Cell Transplant 13:535-47. 2004
    ....
  7. pmc Dopaminergic neurons derived from human induced pluripotent stem cells survive and integrate into 6-OHDA-lesioned rats
    Jingli Cai
    Farber Institute for Neurosciences, Department of Neurology, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Stem Cells Dev 19:1017-23. 2010
    ....
  8. pmc Tyrosine hydroxylase gene regulation in human neuronal progenitor cells does not depend on Nurr1 as in the murine and rat systems
    Hao Jin
    The Farber Institute for the Neurosciences, Department of Neurology, Thomas Jefferson University, Jefferson Hospital for the Neurosciences, Philadelphia, PA 19107, USA
    J Cell Physiol 207:49-57. 2006
    ....
  9. doi request reprint Human amniotic fluid stem cells do not differentiate into dopamine neurons in vitro or after transplantation in vivo
    Angela E Donaldson
    Department of Neurology, Farber Institute for the Neurosciences, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    Stem Cells Dev 18:1003-12. 2009
    ..Taken together, these results suggest that further studies will be needed to improve differentiation procedures in culture and to prolong cell survival in vivo if hAFS cells are to be useful as replacement cells in PD...
  10. ncbi request reprint The human tyrosine hydroxylase gene promoter
    Mark A Kessler
    Department of Neurology and Farber Institute for the Neurosciences, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Brain Res Mol Brain Res 112:8-23. 2003
    ..Within these conserved regions (CRs I-V), potential recognition sites for NR4A2 (Nurr1), HNF-3beta, HOXA4, and HOXA5 were shared across human, mouse, and rat TH promoters...
  11. doi request reprint The role of Lmx1a in the differentiation of human embryonic stem cells into midbrain dopamine neurons in culture and after transplantation into a Parkinson's disease model
    Jingli Cai
    Farber Institute for Neurosciences, Department of Neurology, Thomas Jefferson University Medical College, Philadelphia, Pennsylvania 19107, USA
    Stem Cells 27:220-9. 2009
    ..e., Aldh1a1), may prove to be extremely useful for the selection of appropriately staged and suitably mDA-specified hES cells for cell replacement in Parkinson's disease...
  12. ncbi request reprint Neural stem cells spontaneously express dopaminergic traits after transplantation into the intact or 6-hydroxydopamine-lesioned rat
    Ming Yang
    Department of Neurology, Thomas Jefferson University Medical College, 1025 Walnut Street, Philadelphia, Pennsylvania 19107, USA
    Exp Neurol 177:50-60. 2002
    ....
  13. ncbi request reprint Genetic ablation of caveolin-1 increases neural stem cell proliferation in the subventricular zone (SVZ) of the adult mouse brain
    Jean Francois Jasmin
    Department of Stem Cell Biology and Regenerative Medicine, Thomas Jefferson University, Philadelphia, PA, USA
    Cell Cycle 8:3978-83. 2009
    ..Thus, the SVZ of caveolin KO mouse brains displays an increased proliferation of adult neural stem cells. Caveolin proteins might represent new crucial regulators of adult neural stem cell proliferation...
  14. pmc Purified mouse dopamine neurons thrive and function after transplantation into brain but require novel glial factors for survival in culture
    A E Donaldson
    Farber Institute for Neurosciences, Thomas Jefferson University Medical College, Philadelphia, PA 19107, USA
    Mol Cell Neurosci 30:108-17. 2005
    ..We conclude that GFP-tagged DA neurons can be purified to homogeneity and can survive and function when grown with glial factors in vitro or after transplantation in vivo...
  15. pmc Characterization of five evolutionary conserved regions of the human tyrosine hydroxylase (TH) promoter: implications for the engineering of a human TH minimal promoter assembled in a self-inactivating lentiviral vector system
    Gaetano Romano
    Department of Neurosurgery, The Farber Institute for the Neurosciences, Thomas Jefferson University, Jefferson Hospital for the Neurosciences, Philadelphia, Pennsylvania, USA
    J Cell Physiol 204:666-77. 2005
    ..The human TH minimal promoters may provide the opportunity for the selection of TH-positive human embryonic and adult stem cells for brain transplantation experiments in animal models for Parkinson's disease...
  16. pmc Heterotopically transplanted CVO neural stem cells generate neurons and migrate with SVZ cells in the adult mouse brain
    Lori B Bennett
    Farber Institute for Neurosciences, Department of Neurology, Thomas Jefferson University Medical College, 900 Walnut Street, Philadelphia, PA 19107, USA
    Neurosci Lett 475:1-6. 2010
    ..These findings may have implications for neural repair in the adult brain...
  17. doi request reprint Cell-autonomous alteration of dopaminergic transmission by wild type and mutant (DeltaE) TorsinA in transgenic mice
    Michelle E Page
    Thomas Jefferson University, Department of Neurosurgery, Farber Institute for Neurosciences, Philadelphia, PA 19107, USA
    Neurobiol Dis 39:318-26. 2010
    ..This is the first cell-subtype-specific DYT1 transgenic mouse that can serve to differentiate between primary and secondary changes in dystonia, thereby helping to target disease therapies...
  18. pmc Transcription and epigenetic profile of the promoter, first exon and first intron of the human tyrosine hydroxylase gene
    Gaetano Romano
    Department of Neurosurgery, Temple University, Philadelphia, Pennsylvania, USA
    J Cell Physiol 211:431-8. 2007
    ..Further analysis of epigenetic programming might lead to the identification of the factors that determine TH gene expression specifically in dopaminergic neurons...
  19. pmc Transient differentiation of adult human bone marrow cells into neuron-like cells in culture: development of morphological and biochemical traits is mediated by different molecular mechanisms
    Sokreine Suon
    Farber Institute for Neurosciences, Department of Neurology, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Stem Cells Dev 13:625-35. 2004
    ..These results demonstrate that hMSCs acquire the morphological appearance and the biochemical makeup typical of neurons by independently regulated mechanisms...
  20. ncbi request reprint Antioxidant compounds protect dopamine neurons from death due to oxidative stress in vitro
    Natalie D Stull
    Department of Neurology, Thomas Jefferson University Medical College, Philadelphia, PA 19107, USA
    Brain Res 931:181-5. 2002
    ..We conclude that antioxidants are more effective at preventing cell death in vitro than are inhibitors at later stages in the death cascade...

Research Grants21

  1. Neural Stem Cells Grafts in Primate Models of Parkinsons
    Lorraine Iacovitti; Fiscal Year: 2004
    ....
  2. Using Stem Cells in Animal Models of Parkinson's Disease
    Lorraine Iacovitti; Fiscal Year: 2006
    ..abstract_text> ..
  3. Imaging stem cell implants in neurodegenerative disease
    Lorraine Iacovitti; Fiscal Year: 2007
    ..This will provide vital information in the animal model of PD, allow us to longitudinally follow DA neuron regeneration, and, most importantly, will be translatable to clinical human studies using similar techniques. ..
  4. STUDIES OF PURIFIED DOPAMINE NEURONS
    Lorraine Iacovitti; Fiscal Year: 2002
    ..Knowledge gleaned from these studies will hopefully provide the building blocks needed to devise strategies for the treatment of Parkinson's and other diseases involving compromised DA systems. ..
  5. FACTORS REGULATING TYROSINE HYDROXYLASE GENE EXPRESSION
    Lorraine Iacovitti; Fiscal Year: 2002
    ..abstract_text> ..
  6. STUDIES OF PURIFIED DOPAMINE NEURONS
    Lorraine Iacovitti; Fiscal Year: 2007
    ..This work will hopefully lay the foundation for the development of therapeutic treatments for Parkinson's and other diseases involving compromised DA systems. ..