PAOLO M FORTINA
Affiliation: Thomas Jefferson University
- The human platelet: strong transcriptome correlations among individuals associate weakly with the platelet proteomeEric R Londin
Computational Medicine Center, Thomas Jefferson University, Philadelphia, PA 19107, USA
Biol Direct 9:3. 2014..We profiled the platelet transcriptome of 10 healthy young males (5 white and 5 black) with no notable clinical history using RNA sequencing and by Affymetrix microarray...
- The complex transcriptional landscape of the anucleate human plateletPaul F Bray
Cardeza Foundation for Hematologic Research, Division of Hematology, Department of Medicine, Thomas Jefferson University, Philadelphia, PA, USA
BMC Genomics 14:1. 2013..The goal of this effort was to deep-sequence RNA from leukocyte-depleted platelets to capture the complex profile of all expressed transcripts...
- Revealing genes associated with vitellogenesis in the liver of the zebrafish (Danio rerio) by transcriptome profilingLiraz Levi
Department Marine Biology and Biotechnology, Israel Oceanographic and Limnological Research, Haifa, Israel
BMC Genomics 10:141. 2009....
- Whole-exome sequencing of DNA from peripheral blood mononuclear cells (PBMC) and EBV-transformed lymphocytes from the same donorEric R Londin
Cancer Genomics Laboratory, Kimmel Cancer Center
BMC Genomics 12:464. 2011....
- Applications of nanoparticles to diagnostics and therapeutics in colorectal cancerPaolo Fortina
Department of Cancer Biology, Kimmel Cancer Center, Thomas Jefferson University, Jefferson Medical College, Philadelphia, PA 19107, USA
Trends Biotechnol 25:145-52. 2007..Although the scenarios outlined in this article are hypothetical, they might stimulate ideas about how other cancers could be attacked using nanotechnology...
- Nanobiotechnology: the promise and reality of new approaches to molecular recognitionPaolo Fortina
Center for Translational Medicine, Department of Medicine, Thomas Jefferson University, Philadelphia, PA 19107, USA
Trends Biotechnol 23:168-73. 2005..We also speculate on further pathways in nanotechnology development and the emergence of order in this somewhat chaotic, yet promising, new field...
- Gene expression profiling during the transition to failure in TNF-alpha over-expressing mice demonstrates the development of autoimmune myocarditisZhonghua Tang
Department of Medicine, Center for Translational Medicine, Thomas Jefferson University, Suite 813 College Building, 1025 Walnut Street, Philadelphia, PA 19107, USA
J Mol Cell Cardiol 36:515-30. 2004..The autoimmune response occurs concomitantly with cardiac decompensation and may participate in triggering the transition to failure in TNF1.6 mice...
- Opportunities for near-infrared thermal ablation of colorectal metastases by guanylyl cyclase C-targeted gold nanoshellsScott A Waldman
Thomas Jefferson University, Jefferson Medical College, Department of Pharmacology and Experimental Therapeutics, Philadelphia, PA 19107, USA
Future Oncol 2:705-16. 2006....
- NFκB activation and stimulation of chemokine production in normal human macrophages by the gadolinium-based magnetic resonance contrast agent Omniscan: possible role in the pathogenesis of nephrogenic systemic fibrosisFrancesco Del Galdo
Jefferson Institute of Molecular Medicine, Thomas Jefferson University, Philadelphia, Pennsylvania, USA
Ann Rheum Dis 69:2024-33. 2010..To elucidate the mechanisms responsible for macrophage activation, the effects of the GdBCA Omniscan on normal human macrophage global gene expression, chemokine production and nuclear factor κB (NFκB) activation was examined...
- p21CIP1 attenuates Ras- and c-Myc-dependent breast tumor epithelial mesenchymal transition and cancer stem cell-like gene expression in vivoManran Liu
Kimmel Cancer Center, Department of Cancer Biology, Thomas Jefferson University, Philadelphia, PA 19107, USA
Proc Natl Acad Sci U S A 106:19035-9. 2009..Thus, the in vitro cell culture assays reflect the changes observed in vivo in transgenic mice. These findings establish a link between the loss of p21(CIP1) and the acquisition of breast cancer EMT and stem cell properties in vivo...
- Substrate uptake and metabolism are preserved in hypertrophic caveolin-3 knockout heartsAyanna S Augustus
Dept of Cancer Biology, Thomas Jefferson Univ, 233 S 10th St, BLSB 933, Philadelphia, PA 19107, USA
Am J Physiol Heart Circ Physiol 295:H657-66. 2008..Moreover, a loss of Cav3 mRNA altered the expression of several genes not previously linked to cardiac growth and function. Thus we have identified a number of new target genes associated with the pathogenesis of cardiac hypertrophy...
- The Otto Aufranc Award: Identification of a 4 Mb region on chromosome 17q21 linked to developmental dysplasia of the hip in one 18-member, multigeneration familyGeorge Feldman
Thomas Jefferson University and the Rothman Institute of Orthopaedics, 925 Chestnut Street, 5th Floor, Philadelphia, PA 19107, USA
Clin Orthop Relat Res 468:337-44. 2010..Discovering the genetic basis of the disease would be an important step in understanding the etiology of this disabling condition...
- Genotyping on a thermal gradient DNA chipTomoharu Kajiyama
Department of Pediatrics, The Children s Hospital of Philadelphia and University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
Genome Res 13:467-75. 2003..Unambiguous typing was achieved for clinically relevant loci within the factor VII and hemochromatosis genes...
- Identification of APC gene mutations in colorectal cancer using universal microarray-based combinatorial sequencing-by-hybridizationShannon Cowie
Center for Translational Medicine, Department of Medicine, Thomas Jefferson University, Jefferson Medical College, Philadelphia, Pennsylvania, USA
Hum Mutat 24:261-71. 2004..6 kb from a PCR target, with 99.97% accuracy on a single HyChip trade mark slide. cSBH is a fast, cost-efficient method for first stage mutation screening in the APC or any other gene...
- L-selenomethionine modulates high LET radiation-induced alterations of gene expression in cultured human thyroid cellsJelena Stewart
Department of Radiation Oncology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6072, USA
Oncol Rep 16:569-74. 2006..This study provides a basis for furthering our knowledge about radiation-induced molecular and cellular changes that lead to cellular transformation and death...
- Stat5 promotes metastatic behavior of human prostate cancer cells in vitro and in vivoLei Gu
Cancer Biology, Thomas Jefferson University, Philadelphia, Pennsylvania, USA
Endocr Relat Cancer 17:481-93. 2010..Stat5 may provide a therapeutic target protein for disseminated prostate cancer...
- Transcription factor Stat3 stimulates metastatic behavior of human prostate cancer cells in vivo, whereas Stat5b has a preferential role in the promotion of prostate cancer cell viability and tumor growthLei Gu
Department of Cancer Biology, Medical Oncology, Urology, Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA 19107, USA
Am J Pathol 176:1959-72. 2010..This work provides the first mechanistic comparison of the biological effects induced by transcription factors Stat5a/b versus Stat3 in prostate cancer...
- Reversine enhances generation of progenitor-like cells by dedifferentiation of annulus fibrosus cellsMansi Saraiya
Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, PA, USA
Tissue Eng Part A 16:1443-55. 2010..This agent could be used to generate skeletal progenitor cells to orchestrate the repair of the intervertebral disc...
- Pyrosequencing for detection of mutations in the connexin 26 (GJB2) and mitochondrial 12S RNA (MTRNR1) genes associated with hereditary hearing lossAlessandro Ferraris
Department of Pediatrics, The Children s Hospital of Philadelphia, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, USA
Hum Mutat 20:312-20. 2002....
- Microchips: an all-language literature survey including books and patentsLarry J Kricka
Department of Pathology and Laboratory Medicine, University of Pennsylvania Medical Center, 3400 Spruce Street, Philadelphia, PA 19104, USA
Clin Chem 48:1620-2. 2002
- The reverse Warburg effect: aerobic glycolysis in cancer associated fibroblasts and the tumor stromaStephanos Pavlides
Department of Stem Cell Biology and Regenerative Medicine, Thomas Jefferson University, Philadelphia, PA, USA
Cell Cycle 8:3984-4001. 2009..Thus, an absence of stromal Cav-1 may be a biomarker for the "Reverse Warburg Effect," explaining its powerful predictive value...
- Analysis of clinically relevant single-nucleotide polymorphisms by use of microelectronic array technologyRosa Santacroce
Department of Pediatrics, The Children s Hospital of Philadelphia, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
Clin Chem 48:2124-30. 2002..Microelectronic DNA chip devices represent an emerging technology for genotyping. We developed methods for detection of single-nucleotide polymorphisms (SNPs) in clinically relevant genes...
- Hearts lacking caveolin-1 develop hypertrophy with normal cardiac substrate metabolismAyanna S Augustus
Department of Cancer Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
Cell Cycle 7:2509-18. 2008..In summary, targeted loss of Cav1 produces a unique model of cardiac hypertrophy with normal substrate utilization and expression of genes involved in energy metabolism...
- Combinatorial sequencing-by-hybridization: analysis of the NF1 geneAnnalisa Schirinzi
Center for Translational Medicine, Department of Medicine, Thomas Jefferson University, Jefferson Medical College, Philadelphia, Pennsylvania 19107, USA
Genet Test 10:8-17. 2006..In addition, cSBH analysis of the entire NF1 gene demonstrates reproducibility, efficiency and reduced time; therefore, representing an alternative to extensive DNA sequence characterization...
- Region-specific detection of neuroblastoma loss of heterozygosity at multiple loci simultaneously using a SNP-based tag-array platformJohn M Maris
Division of Oncology, The Children s Hospital of Philadelphia, and Department of Pediatrics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
Genome Res 15:1168-76. 2005....
- Increased amplification efficiency of microchip-based PCR by dynamic surface passivationXing Jian Lou
University of Pennsylvania School of Medicine, Philadelphia, PA, USA
Biotechniques 36:248-52. 2004..It can also be used in combination with static passivation (silicon oxide surface layer) to further improve PCR performance using silicon-glass PCR chips...
- Mutation detection using ligase chain reaction in passivated silicon-glass microchips and microchip capillary electrophoresisXing Jian Lou
University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
Biotechniques 37:392, 394, 396-8. 2004..Ligated primers were detected and quantified simply and conveniently using microchip capillary electrophoresis...
- Kinetics of heterogeneous hybridization on indium tin oxide surfaces with and without an applied potentialHung Ju Su
Department of Pediatrics, The Children s Hospital of Philadelphia, University of Pennsylvania, School of Medicine, Philadelphia, 19104, USA
Electrophoresis 23:1551-7. 2002..This approach may have implications for enhancing array-based hybrid capture for mutation detection, copy number determination and/or gene expression profiling...
- FOUR COLOR ARRAY BOUND SNP/MUTATION DETECTION IN CANCERPaolo Fortina; Fiscal Year: 2004..In addition, it will help establish basic knowledge required to rapidly define the molecular basis of specific malignancies and eventually provide a foundation for rational molecular assessment of therapeutic. ..