Steven A Farber

Summary

Affiliation: Thomas Jefferson University
Country: USA

Publications

  1. ncbi request reprint Genetic analysis of digestive physiology using fluorescent phospholipid reporters
    S A Farber
    Department of Embryology, Carnegie Institution of Washington, Baltimore, MD 21210, USA
    Science 292:1385-8. 2001
  2. pmc The zebrafish annexin gene family
    Steven A Farber
    Department of Embryology, Carnegie Institution of Washington, Baltimore, Maryland 21210, USA
    Genome Res 13:1082-96. 2003
  3. pmc Central and C-terminal domains of heterotrimeric G protein gamma subunits differentially influence the signaling necessary for primordial germ cell migration
    Timothy Mulligan
    Department of Embryology, Carnegie Institution for Science, Baltimore, MD 21218, USA
    Cell Signal 23:1617-24. 2011
  4. pmc In vivo imaging of zebrafish digestive organ function using multiple quenched fluorescent reporters
    Kotaro Hama
    Department of Embriology, Carnegie Institution, Baltimore, MD 21224, USA
    Am J Physiol Gastrointest Liver Physiol 296:G445-53. 2009
  5. pmc Breaking down the stereotypes of science by recruiting young scientists
    Jamie Schaefer
    Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania
    PLoS Biol 2:e279. 2004
  6. ncbi request reprint Sequence specificity of alternating hydroyprolyl/phosphono peptide nucleic acids against zebrafish embryo mRNAs
    E Wickstrom
    Department of Biochemistry and Molecular Pharmacology, Thomas Jefferson University, Philadelphia, PA 19107, USA
    J Drug Target 12:363-72. 2004
  7. pmc A versatile aquatics facility inventory system with real-time barcode scan entry
    Jennifer L Anderson
    Department of Embryology, Carnegie Institution for Science, Baltimore, Maryland 21218, USA
    Zebrafish 7:281-7. 2010
  8. pmc Zebrafish lipid metabolism: from mediating early patterning to the metabolism of dietary fat and cholesterol
    Jennifer L Anderson
    Carnegie Institution for Science, Department of Embryology, Baltimore, Maryland, USA
    Methods Cell Biol 101:111-41. 2011
  9. ncbi request reprint Characterization of Ca2+-dependent phospholipase A2 activity during zebrafish embryogenesis
    S A Farber
    Department of Embryology, Carnegie Institution of Washington, Baltimore, Maryland 21210, USA
    J Biol Chem 274:19338-46. 1999
  10. pmc Visualization of lipid metabolism in the zebrafish intestine reveals a relationship between NPC1L1-mediated cholesterol uptake and dietary fatty acid
    James W Walters
    Department of Embryology, Carnegie Institution for Science, Baltimore, MD 21218, USA
    Chem Biol 19:913-25. 2012

Collaborators

Detail Information

Publications25

  1. ncbi request reprint Genetic analysis of digestive physiology using fluorescent phospholipid reporters
    S A Farber
    Department of Embryology, Carnegie Institution of Washington, Baltimore, MD 21210, USA
    Science 292:1385-8. 2001
    ..Thus, fluorescent lipids provide a sensitive readout of lipid metabolism and are a powerful tool for identifying genes that mediate vertebrate digestive physiology...
  2. pmc The zebrafish annexin gene family
    Steven A Farber
    Department of Embryology, Carnegie Institution of Washington, Baltimore, Maryland 21210, USA
    Genome Res 13:1082-96. 2003
    ..The differences in gene expression of recently duplicated anx genes could explain why highly related paralogs were maintained in the genome and did not rapidly become pseudogenes...
  3. pmc Central and C-terminal domains of heterotrimeric G protein gamma subunits differentially influence the signaling necessary for primordial germ cell migration
    Timothy Mulligan
    Department of Embryology, Carnegie Institution for Science, Baltimore, MD 21218, USA
    Cell Signal 23:1617-24. 2011
    ....
  4. pmc In vivo imaging of zebrafish digestive organ function using multiple quenched fluorescent reporters
    Kotaro Hama
    Department of Embriology, Carnegie Institution, Baltimore, MD 21224, USA
    Am J Physiol Gastrointest Liver Physiol 296:G445-53. 2009
    ..Thus the multiple fluorescent reporter-based methodology facilitates the rapid analysis of digestive organ function in live zebrafish larvae...
  5. pmc Breaking down the stereotypes of science by recruiting young scientists
    Jamie Schaefer
    Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania
    PLoS Biol 2:e279. 2004
  6. ncbi request reprint Sequence specificity of alternating hydroyprolyl/phosphono peptide nucleic acids against zebrafish embryo mRNAs
    E Wickstrom
    Department of Biochemistry and Molecular Pharmacology, Thomas Jefferson University, Philadelphia, PA 19107, USA
    J Drug Target 12:363-72. 2004
    ..These observations validate HypNA-pPNAs as an alternative to MO oligomers for reverse genetic studies. The stronger hybridization and greater specificity of HypNA-pPNAs enable knockdown of mRNAs unaffected by MO oligomers...
  7. pmc A versatile aquatics facility inventory system with real-time barcode scan entry
    Jennifer L Anderson
    Department of Embryology, Carnegie Institution for Science, Baltimore, Maryland 21218, USA
    Zebrafish 7:281-7. 2010
    ....
  8. pmc Zebrafish lipid metabolism: from mediating early patterning to the metabolism of dietary fat and cholesterol
    Jennifer L Anderson
    Carnegie Institution for Science, Department of Embryology, Baltimore, Maryland, USA
    Methods Cell Biol 101:111-41. 2011
    ..Numerous studies have shown that the zebrafish is an excellent model for vertebrate lipid metabolism. In this chapter, we review studies that employ zebrafish to better understand lipid signaling and metabolism...
  9. ncbi request reprint Characterization of Ca2+-dependent phospholipase A2 activity during zebrafish embryogenesis
    S A Farber
    Department of Embryology, Carnegie Institution of Washington, Baltimore, Maryland 21210, USA
    J Biol Chem 274:19338-46. 1999
    ..Biol. Chem. 269, 18239-18249). By using a quenched BODIPY-labeled phosphatidylcholine that fluoresces only upon cleavage by PLA2, lipase activity was visualized in the cells of living embryos where it localized to perinuclear membranes...
  10. pmc Visualization of lipid metabolism in the zebrafish intestine reveals a relationship between NPC1L1-mediated cholesterol uptake and dietary fatty acid
    James W Walters
    Department of Embryology, Carnegie Institution for Science, Baltimore, MD 21218, USA
    Chem Biol 19:913-25. 2012
    ..These data illustrate the power of the zebrafish system to address longstanding questions in vertebrate digestive physiology...
  11. pmc Prenylation-deficient G protein gamma subunits disrupt GPCR signaling in the zebrafish
    Timothy Mulligan
    Carnegie Institution, Department of Embryology, Johns Hopkins University, Baltimore, MD 21218, United States
    Cell Signal 22:221-33. 2010
    ....
  12. pmc Zebrafish fat-free is required for intestinal lipid absorption and Golgi apparatus structure
    Shiu Ying Ho
    Department of Microbiology and Immunology, Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    Cell Metab 3:289-300. 2006
    ..These data indicate that ffr is required for both Golgi structure and vesicular trafficking, and ultimately lipid transport...
  13. ncbi request reprint Revised nomenclature for the mammalian long-chain acyl-CoA synthetase gene family
    Douglas G Mashek
    Departments of Nutrition and Pediatrics, University of North Carolina, Chapel Hill, NC 27599, USA
    J Lipid Res 45:1958-61. 2004
    ..Splice variants of ACSL3, -4, -5, and -6 are cataloged. Suggestions for naming other family members and for the nonmammalian acyl-CoA synthetases are made...
  14. ncbi request reprint Targeted gene knockdown in zebrafish using negatively charged peptide nucleic acid mimics
    Karen A Urtishak
    Department of Microbiology and Immunology and Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    Dev Dyn 228:405-13. 2003
    ....
  15. ncbi request reprint Analysis of small molecule metabolism in zebrafish
    Shiu Ying Ho
    Department of Microbiology and Immunology, Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    Methods Enzymol 364:408-26. 2003
    ..The potential to identify new genes, or novel functions of known genes that regulate dietary lipid metabolism, or the generation of lipid signaling molecules, may lead to the development of treatment strategies for common human diseases...
  16. ncbi request reprint Germ cell migration in zebrafish is dependent on HMGCoA reductase activity and prenylation
    Juanita L Thorpe
    Kimmel Cancer Center, Department of Microbiology and Immunology, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Dev Cell 6:295-302. 2004
    ..Together with data from Drosophila (Santos and Lehmann, this issue), our results highlight a conserved role for protein geranylgeranylation in this context...
  17. pmc Annexin 2-caveolin 1 complex is a target of ezetimibe and regulates intestinal cholesterol transport
    Eric J Smart
    Department of Physiology, University of Kentucky, Lexington, KY 40536, USA
    Proc Natl Acad Sci U S A 101:3450-5. 2004
    ..Pharmacological treatment of mice with ezetimibe disrupts the heterocomplex in only hypercholesterolemic animals. These data suggest that ANX2 and CAV1 are components of an intestinal sterol transport complex...
  18. pmc A web based resource characterizing the zebrafish developmental profile of over 16,000 transcripts
    Ming Ouyang
    Department of Pharmacology, UMDNJ, Piscataway, NJ, USA
    Gene Expr Patterns 8:171-80. 2008
    ....
  19. pmc p53 activation by knockdown technologies
    Mara E Robu
    University of Minnesota, Minneapolis, Minnesota, United States of America
    PLoS Genet 3:e78. 2007
    ..p53 inhibition could potentially be applicable to other systems to suppress off-target effects caused by other knockdown technologies...
  20. pmc Coordinate control of cell cycle regulatory genes in zebrafish development tested by cyclin D1 knockdown with morpholino phosphorodiamidates and hydroxyprolyl-phosphono peptide nucleic acids
    Kevin T Duffy
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, PA, USA
    Nucleic Acids Res 33:4914-21. 2005
    ....
  21. pmc D-glucuronyl C5-epimerase acts in dorso-ventral axis formation in zebrafish
    Giancarlo Ghiselli
    Department of Pathology and Cell Biology, Thomas Jefferson University, Philadelphia, PA 19107, USA
    BMC Dev Biol 5:19. 2005
    ..In this study the role of Glce during early zebrafish development has been investigated...
  22. ncbi request reprint Novel use of zebrafish as a vertebrate model to screen radiation protectors and sensitizers
    Mary Frances McAleer
    Department of Radiation Oncology, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Int J Radiat Oncol Biol Phys 61:10-3. 2005
    ....
  23. ncbi request reprint Tim50, a component of the mitochondrial translocator, regulates mitochondrial integrity and cell death
    Yin Guo
    Center for Apoptosis Research and the Department of Microbiology and Immunology, Kimmel Cancer Institute, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    J Biol Chem 279:24813-25. 2004
    ..Thus Tim50 is important for both mitochondrial function and early neuronal development...
  24. ncbi request reprint Lipid metabolism in zebrafish
    Shiu Ying Ho
    Department of Microbiology and Immunology, Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    Methods Cell Biol 76:87-108. 2004
    ..These zebrafish-based assays are important tools to understand aspects of lipid biology with significant clinical implications...
  25. ncbi request reprint Downregulation of gene expression with negatively charged peptide nucleic acids (PNAs) in zebrafish embryos
    Eric Wickstrom
    Department of Biochemistry and Molecular Pharmacology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    Methods Cell Biol 77:137-58. 2004
    ....