Genomes and Genes
P J Donovan
Affiliation: Thomas Jefferson University
- Germline exclusion of Cks1 in the mouse reveals a metaphase I role for Cks proteins in male and female meiosisPeter J Donovan
Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, Pennslvania 19107, USA
Cell Cycle 2:275-6. 2003
- Turning germ cells into stem cellsPeter J Donovan
Kimmel Cancer Center, Thomas Jefferson University, Bluemle Life Sciences Building, 233 South 10th Street, Philadelphia, Pennsylvania 19107, USA
Curr Opin Genet Dev 13:463-71. 2003..Understanding the genetics of embryonal carcinoma cell formation and the growth factor signaling pathways controlling embryonic germ cell derivation could tell us much about the molecular controls on developmental potency in mammals...
- The end of the beginning for pluripotent stem cellsP J Donovan
Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
Nature 414:92-7. 2001..As such they represent an incredible resource for the repair of diseased or damaged tissues in our bodies. These cells also promise to open a new window into the embryonic development of our species...
- Germ cell biology--from generation to generationP J Donovan
Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA 19107, USA
Int J Dev Biol 45:523-31. 2001..This review will focus on these two key aspects of germ cell biology, developmental potency and meiosis...
- The germ cell--the mother of all stem cellsP J Donovan
Cell Biology of Development and Differentiation Group, ABL Basic Research Program, NCI FCRDC, Frederick MD, USA
Int J Dev Biol 42:1043-50. 1998....
- Lack of germline transmission of vector sequences following systemic administration of recombinant AAV-2 vector in malesV R Arruda
The Children's Hospital of Philadelphia, and Department of Pediatrics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
Mol Ther 4:586-92. 2001..We conclude that the risk of inadvertent germline transmission of vector sequences following IM or hepatic artery injection of AAV-2 vectors is extremely low...
- A highly conserved centrosomal kinase, AIR-1, is required for accurate cell cycle progression and segregation of developmental factors in Caenorhabditis elegans embryosJ M Schumacher
Cell Biology of Development and Differentiation Group, Developmental Signal Transduction Group, ABL Basic Research Program, National Cancer Institute Frederick Cancer Research and Development Center, PO Box B, Frederick, MD 21702, USA
Development 125:4391-402. 1998..Our data suggests AIR-1 plays a role in P-granule segregation and the association of the germline factor PIE-1 with centrosomes...
- Decorin, epiphycan, and lumican genes are closely linked on murine Chromosome 10 and are deleted in lethal steel mutantsK G Danielson
Department of Pathology, Anatomy and Cell Biology, Room 249 Jefferson Alumni Hall, Jefferson Medical College, Thomas Jefferson University, 1020 Locust Street, Philadelphia, Pennsylvania 19107, USA
Mamm Genome 10:201-3. 1999
- Cellular apoptosis susceptibility gene capts maps to mouse chromosome 2M De Miguel
Mammalian Genetics Laboratory, ABL Basic Research Program, NCI FCRDC, PO Box B, Bldg 539, Frederick, Maryland 21702 1201, USA
Mamm Genome 9:411-2. 1998
- The four cdc25 genes from the nematode Caenorhabditis elegansN R Ashcroft
ABL Basic Research Program, Gene Regulation, Chromosome Biology Laboratory, PO Box B, Building 539, National Cancer Institute Frederick Cancer Research, Development Center, Frederick, MD 21702, USA
Gene 214:59-66. 1998..These data demonstrate the value of genome database searching for identifying new members of known gene families, understanding genetic diversity, and for studying gene structure...
- AIR-2: An Aurora/Ipl1-related protein kinase associated with chromosomes and midbody microtubules is required for polar body extrusion and cytokinesis in Caenorhabditis elegans embryosJ M Schumacher
Cell Biology of Development and Differentiation Group, ABL Basic Research Program, NCI Frederick Cancer Research and Development Center, Frederick, Maryland 21702, USA
J Cell Biol 143:1635-46. 1998..Our results suggest that during each meiotic and mitotic division, AIR-2 may coordinate the congression of metaphase chromosomes with the subsequent events of polar body extrusion and cytokinesis...
- A novel mammalian, mitotic spindle-associated kinase is related to yeast and fly chromosome segregation regulatorsG Gopalan
Cell Biology of Development and Differentiation Group, ABL Basic Research Program, National Cancer Institute, Frederick Cancer Research and Development Center, Frederick, Maryland 21702 1201, USA
J Cell Biol 138:643-56. 1997..We suggest that IAK1 is a new member of an emerging subfamily of the serine/threonine kinase superfamily. The members of this subfamily may be important regulators of chromosome segregation...
- Meeting summary: European Workshop on Stem Cells, European Centre for the Validation of Biomedical Testing Methods, Institute for Health and Consumer Protection, Joint Research Centre, Ispra, Italy, November 21-23, 2001Laura Gribaldo
European Centre for the Validation of Biomedical Testing Methods, Institute for Health and Consumer Protection, Joint Research Centre, Ispra, Italy
Exp Hematol 30:628-33. 2002
- Requirement of Cks2 for the first metaphase/anaphase transition of mammalian meiosisCharles H Spruck
Department of Molecular Biology, MB 7, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
Science 300:647-50. 2003....
- Cdc25b phosphatase is required for resumption of meiosis during oocyte maturationA Jeannine Lincoln
National Cancer Institute FCRDC, Frederick, Maryland 21702, USA
Nat Genet 30:446-9. 2002..Cdc25b is therefore essential for meiotic resumption in female mice. Mice lacking Cdc25b provide the first genetic model for studying the mechanisms regulating prophase arrest in vertebrates...
- RAD51C deficiency in mice results in early prophase I arrest in males and sister chromatid separation at metaphase II in femalesSergey Kuznetsov
Mouse Cancer Genetics Program, Center for Cancer Research, National Cancer Institute at Frederick, Frederick, MD 21702, USA
J Cell Biol 176:581-92. 2007..Based on the marked reduction in Holliday junction (HJ) resolution activity in Rad51c-null mouse embryonic fibroblasts, we propose that this late function may be associated with HJ resolution...
- Fez1/Lzts1 absence impairs Cdk1/Cdc25C interaction during mitosis and predisposes mice to cancer developmentAndrea Vecchione
Department of Molecular Virology, Immunology, and Medical Genetics and Comprehensive Cancer Center, Ohio State University, Columbus, OH 43210, USA
Cancer Cell 11:275-89. 2007..Accordingly, Lzts1 deficiency was associated with an increased incidence of both spontaneous and carcinogen-induced cancers in mice...
- Embryonic germ cells: when germ cells become stem cellsCandace L Kerr
Institute for Cell Engineering, Department of Obstetrics and Gynecology, Johns Hopkins University, Baltimore, Maryland 21205, USA
Semin Reprod Med 24:304-13. 2006..Transplantation studies have begun in a variety of models in hopes of defining their potential use to treat a wide variety of human conditions, including diabetes and urological and neurological disorders...
- Staining embryonic stem cells using monoclonal antibodies to stage-specific embryonic antigensBruce A Fenderson
Department of Pathology, Anatomy, and Cell Biology, Thomas Jefferson University, Philadelphia, PA, USA
Methods Mol Biol 325:207-24. 2006....
- Neurotrophins mediate human embryonic stem cell survivalApril D Pyle
Germ Cell and Stem Cell Group, Institute for Cell Engineering, Johns Hopkins University, School of Medicine, Broadway Research Building, Suite 759, 733 North Broadway, Baltimore, Maryland 21205, USA
Nat Biotechnol 24:344-50. 2006..Neurotrophins improve hES cell survival and may facilitate their manipulation and the development of high-throughput screens to identify factors responsible for hES cell differentiation...
- Normal cell cycle and checkpoint responses in mice and cells lacking Cdc25B and Cdc25C protein phosphatasesAngela M Ferguson
Department of Cell Biology and Physiology and Howard Hughes Medical Institute, Washington University School of Medicine, Box 8228, 660 South Euclid Ave, St Louis, MO 63110, USA
Mol Cell Biol 25:2853-60. 2005..These findings indicate that Cdc25A, or possibly other phosphatases, is able to functionally compensate for the loss of Cdc25B and Cdc25C in mice...
- Chipping away at 'stemness'April D Pyle
Germ Cell and Stem Cell Group, Stem Cell Program, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Genome Biol 5:235. 2004..Support remains elusive, however, for the concept of 'stemness'--a pattern of expression of genes that is common to all stem cells...
- Isolation and culture of embryonic germ cellsMaria P De Miguel
Department of Microbiology, Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
Methods Enzymol 365:353-63. 2003
- Dissection of the c-Kit signaling pathway in mouse primordial germ cells by retroviral-mediated gene transferMaria P De Miguel
Kimmel Cancer Center, Thomas Jefferson University, Bluemle Life Sciences Building, Room 706, 233 South 10th Street, Philadelphia, PA 19107, USA
Proc Natl Acad Sci U S A 99:10458-63. 2002..Such technology for manipulating gene expression in PGCs will allow many of the molecular mechanisms regulating germ cell growth, behavior, and differentiation to be comprehensively analyzed...
- Identifying genes differentially expressed between PGCs and ES cells reveals a role for CREB-binding protein in germ cell survivalAaron M Elliott
Department of Microbiology and Immunology, Kimmel Cancer Center, Thomas Jefferson University, 233 South 10th Street, Philadelphia, PA 19107, USA
Dev Biol 311:347-58. 2007..Loss of CBP in PGCs leads to increased apoptosis and subsequent reduction in PGC numbers. These data indicate an essential role for CBP in maintaining normal germ cell development...