Gino Cingolani

Summary

Affiliation: Thomas Jefferson University
Country: USA

Publications

  1. pmc Structure of the ATP synthase catalytic complex (F(1)) from Escherichia coli in an autoinhibited conformation
    Gino Cingolani
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, Pennsylvania, USA
    Nat Struct Mol Biol 18:701-7. 2011
  2. pmc Phosphorylation meets nuclear import: a review
    Jonathan D Nardozzi
    Dept, of Biochemistry and Molecular Biology, Thomas Jefferson University, 233 South 10th Street, Philadelphia, PA 19107, USA
    Cell Commun Signal 8:32. 2010
  3. pmc Small terminase couples viral DNA binding to genome-packaging ATPase activity
    Ankoor Roy
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, 233 South 10th Street, Philadelphia, PA 19107, USA
    Structure 20:1403-13. 2012
  4. doi request reprint Conformational selection in the recognition of the snurportin importin beta binding domain by importin beta
    Anshul Bhardwaj
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, 233 South 10th Street, Philadelphia, Pennsylvania 19107, USA
    Biochemistry 49:5042-7. 2010
  5. pmc A minimal nuclear localization signal (NLS) in human phospholipid scramblase 4 that binds only the minor NLS-binding site of importin alpha1
    Kaylen Lott
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    J Biol Chem 286:28160-9. 2011
  6. pmc Dimerization of Vaccinia virus VH1 is essential for dephosphorylation of STAT1 at tyrosine 701
    Adem C Koksal
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    J Biol Chem 286:14373-82. 2011
  7. pmc Improved crystallization of Escherichia coli ATP synthase catalytic complex (F1) by introducing a phosphomimetic mutation in subunit ε
    Ankoor Roy
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Acta Crystallogr Sect F Struct Biol Cryst Commun 68:1229-33. 2012
  8. pmc Nucleoporin Nup50 stabilizes closed conformation of armadillo repeat 10 in importin α5
    Ruth A Pumroy
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    J Biol Chem 287:2022-31. 2012
  9. pmc Atomic structure of dual-specificity phosphatase 26, a novel p53 phosphatase
    Ravi Kumar Lokareddy
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, 233 South 10th Street, Philadelphia, Pennsylvania 19107, United States
    Biochemistry 52:938-48. 2013
  10. pmc Structure of p22 headful packaging nuclease
    Ankoor Roy
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    J Biol Chem 287:28196-205. 2012

Collaborators

  • Victor P T Pau
  • Sherwood Casjens
  • Uwe Vinkemeier
  • Nelly Pante
  • Noriko Yasuhara
  • Anshul Bhardwaj
  • Ankoor Roy
  • Kaylen Lott
  • Jonathan D Nardozzi
  • Adem C Koksal
  • Ravi Kumar Lokareddy
  • Ruth A Pumroy
  • Frank J Smith
  • Jonathan Nardozzi
  • Adam S Olia
  • Gabriel C Lander
  • Brad S Rothberg
  • Darren J Hart
  • Michael J Root
  • Pinaki Datta
  • Thomas M Duncan
  • Marcus L Hutcheon
  • Ian J Molineux
  • Peter J Sims
  • Gregory Mitrousis
  • Nikola Wenta

Detail Information

Publications18

  1. pmc Structure of the ATP synthase catalytic complex (F(1)) from Escherichia coli in an autoinhibited conformation
    Gino Cingolani
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, Pennsylvania, USA
    Nat Struct Mol Biol 18:701-7. 2011
    ..As a result, the three catalytic subunits are stabilized in a set of conformations and rotational positions distinct from previous F(1) structures...
  2. pmc Phosphorylation meets nuclear import: a review
    Jonathan D Nardozzi
    Dept, of Biochemistry and Molecular Biology, Thomas Jefferson University, 233 South 10th Street, Philadelphia, PA 19107, USA
    Cell Commun Signal 8:32. 2010
    ..This is only now beginning to emerge as a key regulatory step in biology...
  3. pmc Small terminase couples viral DNA binding to genome-packaging ATPase activity
    Ankoor Roy
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, 233 South 10th Street, Philadelphia, PA 19107, USA
    Structure 20:1403-13. 2012
    ..This posits a model for DNA-dependent activation of genome-packaging motors of general interest in virology...
  4. doi request reprint Conformational selection in the recognition of the snurportin importin beta binding domain by importin beta
    Anshul Bhardwaj
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, 233 South 10th Street, Philadelphia, Pennsylvania 19107, USA
    Biochemistry 49:5042-7. 2010
    ..We propose the population selection may be a general mechanism used by beta-karyopherins to recognize transport substrates...
  5. pmc A minimal nuclear localization signal (NLS) in human phospholipid scramblase 4 that binds only the minor NLS-binding site of importin alpha1
    Kaylen Lott
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    J Biol Chem 286:28160-9. 2011
    ..These data provide the first structural and functional evidence of a novel NLS-binding mode in importin α1 that uses only the minor groove as the exclusive site for nuclear import of nonclassical cargos...
  6. pmc Dimerization of Vaccinia virus VH1 is essential for dephosphorylation of STAT1 at tyrosine 701
    Adem C Koksal
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    J Biol Chem 286:14373-82. 2011
    ..We propose that dimerization may represent a common mechanism to regulate the activity and substrate recognition of DSPs, often assumed to function as monomers...
  7. pmc Improved crystallization of Escherichia coli ATP synthase catalytic complex (F1) by introducing a phosphomimetic mutation in subunit ε
    Ankoor Roy
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Acta Crystallogr Sect F Struct Biol Cryst Commun 68:1229-33. 2012
    ..coli F(1) that diffract to ∼3.15 Å resolution...
  8. pmc Nucleoporin Nup50 stabilizes closed conformation of armadillo repeat 10 in importin α5
    Ruth A Pumroy
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    J Biol Chem 287:2022-31. 2012
    ..This is the first evidence for a nucleoporin modulating the import reaction by directly altering the three-dimensional structure of an import adaptor...
  9. pmc Atomic structure of dual-specificity phosphatase 26, a novel p53 phosphatase
    Ravi Kumar Lokareddy
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, 233 South 10th Street, Philadelphia, Pennsylvania 19107, United States
    Biochemistry 52:938-48. 2013
    ..The structural characterization of DUSP26 presented in this paper provides the first atomic insight into this disease-associated phosphatase...
  10. pmc Structure of p22 headful packaging nuclease
    Ankoor Roy
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    J Biol Chem 287:28196-205. 2012
    ..This cross-talk allows for precise and controlled cleavage of DNA that is essential for genome packaging...
  11. ncbi request reprint Structure of phage P22 cell envelope–penetrating needle
    Adam S Olia
    Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, 750 East Adams Street, Syracuse, New York 13210, USA
    Nat Struct Mol Biol 14:1221-6. 2007
    ..The slender conformation of the gp26 fiber minimizes the surface exposed to solvent, which is consistent with the idea that gp26 traverses the cell envelope lipid bilayers...
  12. pmc The importin β binding domain as a master regulator of nucleocytoplasmic transport
    Kaylen Lott
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, 233 South 10th Street, Philadelphia, PA 19107, USA
    Biochim Biophys Acta 1813:1578-92. 2011
    ..This article is part of a Special Issue entitled: Regulation of Signaling and Cellular Fate through Modulation of Nuclear Protein Import...
  13. pmc Molecular basis for the recognition of phosphorylated STAT1 by importin alpha5
    Jonathan Nardozzi
    Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, 750 East Adams Street, Syracuse, NY 13210, USA
    J Mol Biol 402:83-100. 2010
    ....
  14. pmc Crystal structure of a Ba(2+)-bound gating ring reveals elementary steps in RCK domain activation
    Frank J Smith
    Department of Biochemistry, Temple University School of Medicine, 3400 N Broad Street, Philadelphia, PA 19140, USA
    Structure 20:2038-47. 2012
    ..Our results suggest a working hypothesis for a sequence of ligand-dependent conformational changes that may underlie RCK domain activation and channel gating...
  15. pmc Crystallization of the nonameric small terminase subunit of bacteriophage P22
    Ankoor Roy
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, 233 South 10th Street, Philadelphia, PA 19107, USA
    Acta Crystallogr Sect F Struct Biol Cryst Commun 67:104-10. 2011
    ..75 Å resolution. Preliminary crystallographic analysis of crystal form II confirms that the S-terminase crystals contain a nonamer in the asymmetric unit and are suitable for high-resolution structure determination...
  16. pmc The importin beta binding domain modulates the avidity of importin beta for the nuclear pore complex
    Kaylen Lott
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    J Biol Chem 285:13769-80. 2010
    ..We propose that by controlling the degree of strain in the tertiary structure of importin beta, the IBB domain modulates the affinity of the import complex for nucleoporins, thus dictating its persistence inside the NPC...
  17. pmc Atomic structure of bacteriophage Sf6 tail needle knob
    Anshul Bhardwaj
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    J Biol Chem 286:30867-77. 2011
    ..In analogy to P22 gp26, we suggest the tail needle of phage Sf6 is ejected through the bacterial cell envelope during infection and its C-terminal knob is threaded through peptidoglycan pores formed by glycan strands...
  18. pmc Dimeric quaternary structure of the prototypical dual specificity phosphatase VH1
    Adem C Koksal
    Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, Syracuse, New York 13210, USA
    J Biol Chem 284:10129-37. 2009
    ..We propose that the dimeric quaternary structure of VH1 is essential for specific recognition of activated STAT1, which prevents its nuclear translocation, thus blocking interferon-gamma signal transduction and antiviral response...

Research Grants6

  1. Molecular Basis for Recognition and Nuclear Import of Non-classical NLS Cargos
    Gino Cingolani; Fiscal Year: 2007
    ..Carry out a quantitative structural and functional analysis of HIV-1 Rev-NLS 3. Define the molecular basis for the recognition of STAT1 "dinner-specific" NLS by importin a-5. ..
  2. Molecular Basis for Recognition and Nuclear Import of Non-classical NLS Cargos
    Gino Cingolani; Fiscal Year: 2010
    ..Carry out a quantitative structural and functional analysis of HIV-1 Rev-NLS 3. Define the molecular basis for the recognition of STAT1 "dinner-specific"NLS by importin a-5. ..