Gino Cingolani

Summary

Affiliation: Thomas Jefferson University
Country: USA

Publications

  1. pmc Three-dimensional structure of a viral genome-delivery portal vertex
    Adam S Olia
    Department of Biological Sciences, Purdue University, West Lafayette, Indiana, USA
    Nat Struct Mol Biol 18:597-603. 2011
  2. pmc Phosphorylation meets nuclear import: a review
    Jonathan D Nardozzi
    Dept, of Biochemistry and Molecular Biology, Thomas Jefferson University, 233 South 10th Street, Philadelphia, PA 19107, USA
    Cell Commun Signal 8:32. 2010
  3. pmc Structure of the ATP synthase catalytic complex (F(1)) from Escherichia coli in an autoinhibited conformation
    Gino Cingolani
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, Pennsylvania, USA
    Nat Struct Mol Biol 18:701-7. 2011
  4. ncbi request reprint Role of gene 10 protein in the hierarchical assembly of the bacteriophage P22 portal vertex structure
    Adam S Olia
    Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, 750 E Adams Street, Syracuse, New York 13210, USA
    Biochemistry 46:8776-84. 2007
  5. pmc Foldon-guided self-assembly of ultra-stable protein fibers
    Anshul Bhardwaj
    Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, Syracuse, New York 13210, USA
    Protein Sci 17:1475-85. 2008
  6. doi request reprint Structural basis of allosteric interactions among Ca2+-binding sites in a K+ channel RCK domain
    Frank J Smith
    Department of Biochemistry, Temple University School of Medicine, 3400 North Broad Street, Philadelphia, Pennsylvania 19140, USA
    Nat Commun 4:2621. 2013
  7. pmc Small terminase couples viral DNA binding to genome-packaging ATPase activity
    Ankoor Roy
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, 233 South 10th Street, Philadelphia, PA 19107, USA
    Structure 20:1403-13. 2012
  8. ncbi request reprint Conformational selection in the recognition of the snurportin importin beta binding domain by importin beta
    Anshul Bhardwaj
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, 233 South 10th Street, Philadelphia, Pennsylvania 19107, USA
    Biochemistry 49:5042-7. 2010
  9. pmc The importin beta binding domain modulates the avidity of importin beta for the nuclear pore complex
    Kaylen Lott
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    J Biol Chem 285:13769-80. 2010
  10. pmc An evolutionarily conserved family of virion tail needles related to bacteriophage P22 gp26: correlation between structural stability and length of the alpha-helical trimeric coiled coil
    Anshul Bhardwaj
    Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, 750 E Adams Street, Syracuse, NY 13210, USA
    J Mol Biol 391:227-45. 2009

Collaborators

Detail Information

Publications30

  1. pmc Three-dimensional structure of a viral genome-delivery portal vertex
    Adam S Olia
    Department of Biological Sciences, Purdue University, West Lafayette, Indiana, USA
    Nat Struct Mol Biol 18:597-603. 2011
    ..We propose that the barrel domain facilitates genome spooling onto the interior surface of the capsid during genome packaging and, in analogy to a rifle barrel, increases the accuracy of genome ejection into the host cell...
  2. pmc Phosphorylation meets nuclear import: a review
    Jonathan D Nardozzi
    Dept, of Biochemistry and Molecular Biology, Thomas Jefferson University, 233 South 10th Street, Philadelphia, PA 19107, USA
    Cell Commun Signal 8:32. 2010
    ..This is only now beginning to emerge as a key regulatory step in biology...
  3. pmc Structure of the ATP synthase catalytic complex (F(1)) from Escherichia coli in an autoinhibited conformation
    Gino Cingolani
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, Pennsylvania, USA
    Nat Struct Mol Biol 18:701-7. 2011
    ..As a result, the three catalytic subunits are stabilized in a set of conformations and rotational positions distinct from previous F(1) structures...
  4. ncbi request reprint Role of gene 10 protein in the hierarchical assembly of the bacteriophage P22 portal vertex structure
    Adam S Olia
    Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, 750 E Adams Street, Syracuse, New York 13210, USA
    Biochemistry 46:8776-84. 2007
    ..The assembled portal vertex structure represents both a membrane-binding and penetrating device as well as a plug that retains the pressurized phage DNA inside the capsid...
  5. pmc Foldon-guided self-assembly of ultra-stable protein fibers
    Anshul Bhardwaj
    Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, Syracuse, New York 13210, USA
    Protein Sci 17:1475-85. 2008
    ....
  6. doi request reprint Structural basis of allosteric interactions among Ca2+-binding sites in a K+ channel RCK domain
    Frank J Smith
    Department of Biochemistry, Temple University School of Medicine, 3400 North Broad Street, Philadelphia, Pennsylvania 19140, USA
    Nat Commun 4:2621. 2013
    ....
  7. pmc Small terminase couples viral DNA binding to genome-packaging ATPase activity
    Ankoor Roy
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, 233 South 10th Street, Philadelphia, PA 19107, USA
    Structure 20:1403-13. 2012
    ..This posits a model for DNA-dependent activation of genome-packaging motors of general interest in virology...
  8. ncbi request reprint Conformational selection in the recognition of the snurportin importin beta binding domain by importin beta
    Anshul Bhardwaj
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, 233 South 10th Street, Philadelphia, Pennsylvania 19107, USA
    Biochemistry 49:5042-7. 2010
    ..We propose the population selection may be a general mechanism used by beta-karyopherins to recognize transport substrates...
  9. pmc The importin beta binding domain modulates the avidity of importin beta for the nuclear pore complex
    Kaylen Lott
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    J Biol Chem 285:13769-80. 2010
    ..We propose that by controlling the degree of strain in the tertiary structure of importin beta, the IBB domain modulates the affinity of the import complex for nucleoporins, thus dictating its persistence inside the NPC...
  10. pmc An evolutionarily conserved family of virion tail needles related to bacteriophage P22 gp26: correlation between structural stability and length of the alpha-helical trimeric coiled coil
    Anshul Bhardwaj
    Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, 750 E Adams Street, Syracuse, NY 13210, USA
    J Mol Biol 391:227-45. 2009
    ..Thus, the overall stability of these bacteriophage fibers is not solely dependent on the number of trimerization repeats in the alpha-helical core...
  11. pmc Structural plasticity of the phage P22 tail needle gp26 probed with xenon gas
    Adam S Olia
    Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, Syracuse, New York 13210, USA
    Protein Sci 18:537-48. 2009
    ..The C-terminal tip may be important in scanning the bacterial surface in search of a cell-envelope penetration site, or for recognition of a yet unidentified receptor on the surface of the host...
  12. pmc Molecular basis for the recognition of phosphorylated STAT1 by importin alpha5
    Jonathan Nardozzi
    Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, 750 East Adams Street, Syracuse, NY 13210, USA
    J Mol Biol 402:83-100. 2010
    ....
  13. pmc A minimal nuclear localization signal (NLS) in human phospholipid scramblase 4 that binds only the minor NLS-binding site of importin alpha1
    Kaylen Lott
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    J Biol Chem 286:28160-9. 2011
    ..These data provide the first structural and functional evidence of a novel NLS-binding mode in importin α1 that uses only the minor groove as the exclusive site for nuclear import of nonclassical cargos...
  14. pmc Dimerization of Vaccinia virus VH1 is essential for dephosphorylation of STAT1 at tyrosine 701
    Adem C Koksal
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    J Biol Chem 286:14373-82. 2011
    ..We propose that dimerization may represent a common mechanism to regulate the activity and substrate recognition of DSPs, often assumed to function as monomers...
  15. pmc Dimeric quaternary structure of the prototypical dual specificity phosphatase VH1
    Adem C Koksal
    Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, Syracuse, New York 13210, USA
    J Biol Chem 284:10129-37. 2009
    ..We propose that the dimeric quaternary structure of VH1 is essential for specific recognition of activated STAT1, which prevents its nuclear translocation, thus blocking interferon-gamma signal transduction and antiviral response...
  16. pmc Atomic structure of dual-specificity phosphatase 26, a novel p53 phosphatase
    Ravi Kumar Lokareddy
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, 233 South 10th Street, Philadelphia, Pennsylvania 19107, United States
    Biochemistry 52:938-48. 2013
    ..The structural characterization of DUSP26 presented in this paper provides the first atomic insight into this disease-associated phosphatase...
  17. ncbi request reprint Structure of phage P22 cell envelope–penetrating needle
    Adam S Olia
    Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, 750 East Adams Street, Syracuse, New York 13210, USA
    Nat Struct Mol Biol 14:1221-6. 2007
    ..The slender conformation of the gp26 fiber minimizes the surface exposed to solvent, which is consistent with the idea that gp26 traverses the cell envelope lipid bilayers...
  18. pmc Improved crystallization of Escherichia coli ATP synthase catalytic complex (F1) by introducing a phosphomimetic mutation in subunit ε
    Ankoor Roy
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Acta Crystallogr Sect F Struct Biol Cryst Commun 68:1229-33. 2012
    ..coli F(1) that diffract to ∼3.15 Å resolution...
  19. pmc Nucleoporin Nup50 stabilizes closed conformation of armadillo repeat 10 in importin α5
    Ruth A Pumroy
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    J Biol Chem 287:2022-31. 2012
    ..This is the first evidence for a nucleoporin modulating the import reaction by directly altering the three-dimensional structure of an import adaptor...
  20. pmc Structure of p22 headful packaging nuclease
    Ankoor Roy
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    J Biol Chem 287:28196-205. 2012
    ..This cross-talk allows for precise and controlled cleavage of DNA that is essential for genome packaging...
  21. pmc Atomic structure of bacteriophage Sf6 tail needle knob
    Anshul Bhardwaj
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    J Biol Chem 286:30867-77. 2011
    ..In analogy to P22 gp26, we suggest the tail needle of phage Sf6 is ejected through the bacterial cell envelope during infection and its C-terminal knob is threaded through peptidoglycan pores formed by glycan strands...
  22. pmc Crystallization of the nonameric small terminase subunit of bacteriophage P22
    Ankoor Roy
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, 233 South 10th Street, Philadelphia, PA 19107, USA
    Acta Crystallogr Sect F Struct Biol Cryst Commun 67:104-10. 2011
    ..75 Å resolution. Preliminary crystallographic analysis of crystal form II confirms that the S-terminase crystals contain a nonamer in the asymmetric unit and are suitable for high-resolution structure determination...
  23. pmc The importin β binding domain as a master regulator of nucleocytoplasmic transport
    Kaylen Lott
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, 233 South 10th Street, Philadelphia, PA 19107, USA
    Biochim Biophys Acta 1813:1578-92. 2011
    ..This article is part of a Special Issue entitled: Regulation of Signaling and Cellular Fate through Modulation of Nuclear Protein Import...
  24. pmc Structure of human PIR1, an atypical dual-specificity phosphatase
    Rajeshwer Singh Sankhala
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, 233 South 10th Street, Philadelphia, Pennsylvania 19107, United States
    Biochemistry 53:862-71. 2014
    ..Thus, a deep active site crevice, two active site ions, and conserved P-loop residues stabilizing the γ-phosphate of RNA are defining features of atypical DSPs that specialize in dephosphorylating 5'-RNA. ..
  25. pmc Crystal structure of a Ba(2+)-bound gating ring reveals elementary steps in RCK domain activation
    Frank J Smith
    Department of Biochemistry, Temple University School of Medicine, 3400 N Broad Street, Philadelphia, PA 19140, USA
    Structure 20:2038-47. 2012
    ..Our results suggest a working hypothesis for a sequence of ligand-dependent conformational changes that may underlie RCK domain activation and channel gating...
  26. doi request reprint Molecular basis for the recognition of snurportin 1 by importin beta
    Gregory Mitrousis
    Department of Biochemistry and Molecular Biology, State University of New York SUNY Upstate Medical University, Syracuse, New York 13210, USA
    J Biol Chem 283:7877-84. 2008
    ..We propose that in vivo the synergy of Nup153 and nuclear RanGTP promotes translocation of uridine-rich ribonucleoproteins into the nucleus...
  27. ncbi request reprint Domain organization and polarity of tail needle GP26 in the portal vertex structure of bacteriophage P22
    Anshul Bhardwaj
    Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, 750, E Adams Street, Syracuse, NY 13210, USA
    J Mol Biol 371:374-87. 2007
    ..We propose that domains III and IV of the tail needle form the tip utilized by the phage to penetrate the host cell wall...
  28. pmc Exploring the atomic structure and conformational flexibility of a 320 Å long engineered viral fiber using X-ray crystallography
    Anshul Bhardwaj
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, 233 South 10th Street, Philadelphia, PA 19107, USA
    Acta Crystallogr D Biol Crystallogr 70:342-53. 2014
    ....
  29. ncbi request reprint Binding-induced stabilization and assembly of the phage P22 tail accessory factor gp4
    Adam S Olia
    Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, 750, E Adams Street, Syracuse, NY 13210, USA
    J Mol Biol 363:558-76. 2006
    ....
  30. pmc Architecture of viral genome-delivery molecular machines
    Anshul Bhardwaj
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, 233 South 10th Street, Philadelphia, PA 19107, USA
    Curr Opin Struct Biol 25:1-8. 2014
    ..The structure of specialized tail fibers and their putative role in host anchoring, cell-surface penetration and genome-ejection is discussed. ..

Research Grants6

  1. Molecular Basis for Recognition and Nuclear Import of Non-classical NLS Cargos
    Gino Cingolani; Fiscal Year: 2006
    ..Carry out a quantitative structural and functional analysis of HIV-1 Rev-NLS 3. Define the molecular basis for the recognition of STAT1 "dinner-specific" NLS by importin a-5. ..
  2. Molecular Basis for Recognition and Nuclear Import of Non-classical NLS Cargos
    Gino Cingolani; Fiscal Year: 2007
    ..Carry out a quantitative structural and functional analysis of HIV-1 Rev-NLS 3. Define the molecular basis for the recognition of STAT1 "dinner-specific" NLS by importin a-5. ..
  3. Molecular Basis for Recognition and Nuclear Import of Non-classical NLS Cargos
    Gino Cingolani; Fiscal Year: 2009
    ..Carry out a quantitative structural and functional analysis of HIV-1 Rev-NLS 3. Define the molecular basis for the recognition of STAT1 "dinner-specific"NLS by importin a-5. ..
  4. Molecular Basis for Recognition and Nuclear Import of Non-classical NLS Cargos
    Gino Cingolani; Fiscal Year: 2010
    ..Carry out a quantitative structural and functional analysis of HIV-1 Rev-NLS 3. Define the molecular basis for the recognition of STAT1 "dinner-specific"NLS by importin a-5. ..
  5. Molecular Basis for Recognition and Nuclear Import of Non-classical NLS Cargos
    Gino Cingolani; Fiscal Year: 2009
    ..Carry out a quantitative structural and functional analysis of HIV-1 Rev-NLS 3. Define the molecular basis for the recognition of STAT1 "dinner-specific"NLS by importin a-5. ..