Gino Cingolani

Summary

Affiliation: Thomas Jefferson University
Country: USA

Publications

  1. ncbi Phosphorylation meets nuclear import: a review
    Jonathan D Nardozzi
    Dept, of Biochemistry and Molecular Biology, Thomas Jefferson University, 233 South 10th Street, Philadelphia, PA 19107, USA
    Cell Commun Signal 8:32. 2010
  2. ncbi Structure of the ATP synthase catalytic complex (F(1)) from Escherichia coli in an autoinhibited conformation
    Gino Cingolani
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, Pennsylvania, USA
    Nat Struct Mol Biol 18:701-7. 2011
  3. ncbi Small terminase couples viral DNA binding to genome-packaging ATPase activity
    Ankoor Roy
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, 233 South 10th Street, Philadelphia, PA 19107, USA
    Structure 20:1403-13. 2012
  4. ncbi Conformational selection in the recognition of the snurportin importin beta binding domain by importin beta
    Anshul Bhardwaj
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, 233 South 10th Street, Philadelphia, Pennsylvania 19107, USA
    Biochemistry 49:5042-7. 2010
  5. ncbi A minimal nuclear localization signal (NLS) in human phospholipid scramblase 4 that binds only the minor NLS-binding site of importin alpha1
    Kaylen Lott
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    J Biol Chem 286:28160-9. 2011
  6. ncbi Dimerization of Vaccinia virus VH1 is essential for dephosphorylation of STAT1 at tyrosine 701
    Adem C Koksal
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    J Biol Chem 286:14373-82. 2011
  7. ncbi Improved crystallization of Escherichia coli ATP synthase catalytic complex (F1) by introducing a phosphomimetic mutation in subunit ε
    Ankoor Roy
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Acta Crystallogr Sect F Struct Biol Cryst Commun 68:1229-33. 2012
  8. ncbi Nucleoporin Nup50 stabilizes closed conformation of armadillo repeat 10 in importin α5
    Ruth A Pumroy
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    J Biol Chem 287:2022-31. 2012
  9. ncbi Structure of p22 headful packaging nuclease
    Ankoor Roy
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    J Biol Chem 287:28196-205. 2012
  10. ncbi Structure of phage P22 cell envelope–penetrating needle
    Adam S Olia
    Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, 750 East Adams Street, Syracuse, New York 13210, USA
    Nat Struct Mol Biol 14:1221-6. 2007

Research Grants

  1. Molecular Basis for Recognition and Nuclear Import of Non-classical NLS Cargos
    Gino Cingolani; Fiscal Year: 2007
  2. Molecular Basis for Recognition and Nuclear Import of Non-classical NLS Cargos
    Gino Cingolani; Fiscal Year: 2010

Collaborators

  • Sherwood Casjens
  • Uwe Vinkemeier
  • Nelly Pante
  • Noriko Yasuhara
  • Anshul Bhardwaj
  • Ankoor Roy
  • Kaylen Lott
  • Jonathan D Nardozzi
  • Adem C Koksal
  • Ravi Kumar Lokareddy
  • Ruth A Pumroy
  • Jonathan Nardozzi
  • Adam S Olia
  • Marcus L Hutcheon
  • Darren J Hart
  • Michael J Root
  • Gabriel C Lander
  • Pinaki Datta
  • Thomas M Duncan
  • Ian J Molineux
  • Peter J Sims
  • Gregory Mitrousis
  • Nikola Wenta

Detail Information

Publications17

  1. ncbi Phosphorylation meets nuclear import: a review
    Jonathan D Nardozzi
    Dept, of Biochemistry and Molecular Biology, Thomas Jefferson University, 233 South 10th Street, Philadelphia, PA 19107, USA
    Cell Commun Signal 8:32. 2010
    ..This is only now beginning to emerge as a key regulatory step in biology...
  2. ncbi Structure of the ATP synthase catalytic complex (F(1)) from Escherichia coli in an autoinhibited conformation
    Gino Cingolani
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, Pennsylvania, USA
    Nat Struct Mol Biol 18:701-7. 2011
    ..As a result, the three catalytic subunits are stabilized in a set of conformations and rotational positions distinct from previous F(1) structures...
  3. ncbi Small terminase couples viral DNA binding to genome-packaging ATPase activity
    Ankoor Roy
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, 233 South 10th Street, Philadelphia, PA 19107, USA
    Structure 20:1403-13. 2012
    ..This posits a model for DNA-dependent activation of genome-packaging motors of general interest in virology...
  4. ncbi Conformational selection in the recognition of the snurportin importin beta binding domain by importin beta
    Anshul Bhardwaj
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, 233 South 10th Street, Philadelphia, Pennsylvania 19107, USA
    Biochemistry 49:5042-7. 2010
    ..We propose the population selection may be a general mechanism used by beta-karyopherins to recognize transport substrates...
  5. ncbi A minimal nuclear localization signal (NLS) in human phospholipid scramblase 4 that binds only the minor NLS-binding site of importin alpha1
    Kaylen Lott
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    J Biol Chem 286:28160-9. 2011
    ..These data provide the first structural and functional evidence of a novel NLS-binding mode in importin α1 that uses only the minor groove as the exclusive site for nuclear import of nonclassical cargos...
  6. ncbi Dimerization of Vaccinia virus VH1 is essential for dephosphorylation of STAT1 at tyrosine 701
    Adem C Koksal
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    J Biol Chem 286:14373-82. 2011
    ..We propose that dimerization may represent a common mechanism to regulate the activity and substrate recognition of DSPs, often assumed to function as monomers...
  7. ncbi Improved crystallization of Escherichia coli ATP synthase catalytic complex (F1) by introducing a phosphomimetic mutation in subunit ε
    Ankoor Roy
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Acta Crystallogr Sect F Struct Biol Cryst Commun 68:1229-33. 2012
    ..coli F(1) that diffract to ∼3.15 Å resolution...
  8. ncbi Nucleoporin Nup50 stabilizes closed conformation of armadillo repeat 10 in importin α5
    Ruth A Pumroy
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    J Biol Chem 287:2022-31. 2012
    ..This is the first evidence for a nucleoporin modulating the import reaction by directly altering the three-dimensional structure of an import adaptor...
  9. ncbi Structure of p22 headful packaging nuclease
    Ankoor Roy
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    J Biol Chem 287:28196-205. 2012
    ..This cross-talk allows for precise and controlled cleavage of DNA that is essential for genome packaging...
  10. ncbi Structure of phage P22 cell envelope–penetrating needle
    Adam S Olia
    Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, 750 East Adams Street, Syracuse, New York 13210, USA
    Nat Struct Mol Biol 14:1221-6. 2007
    ..The slender conformation of the gp26 fiber minimizes the surface exposed to solvent, which is consistent with the idea that gp26 traverses the cell envelope lipid bilayers...
  11. ncbi The importin β binding domain as a master regulator of nucleocytoplasmic transport
    Kaylen Lott
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, 233 South 10th Street, Philadelphia, PA 19107, USA
    Biochim Biophys Acta 1813:1578-92. 2011
    ..This article is part of a Special Issue entitled: Regulation of Signaling and Cellular Fate through Modulation of Nuclear Protein Import...
  12. ncbi Molecular basis for the recognition of phosphorylated STAT1 by importin alpha5
    Jonathan Nardozzi
    Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, 750 East Adams Street, Syracuse, NY 13210, USA
    J Mol Biol 402:83-100. 2010
    ....
  13. ncbi Crystallization of the nonameric small terminase subunit of bacteriophage P22
    Ankoor Roy
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, 233 South 10th Street, Philadelphia, PA 19107, USA
    Acta Crystallogr Sect F Struct Biol Cryst Commun 67:104-10. 2011
    ..75 Å resolution. Preliminary crystallographic analysis of crystal form II confirms that the S-terminase crystals contain a nonamer in the asymmetric unit and are suitable for high-resolution structure determination...
  14. ncbi The importin beta binding domain modulates the avidity of importin beta for the nuclear pore complex
    Kaylen Lott
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    J Biol Chem 285:13769-80. 2010
    ..We propose that by controlling the degree of strain in the tertiary structure of importin beta, the IBB domain modulates the affinity of the import complex for nucleoporins, thus dictating its persistence inside the NPC...
  15. ncbi Atomic structure of dual-specificity phosphatase 26, a novel p53 phosphatase
    Ravi Kumar Lokareddy
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, 233 South 10th Street, Philadelphia, Pennsylvania 19107, United States
    Biochemistry 52:938-48. 2013
    ..The structural characterization of DUSP26 presented in this paper provides the first atomic insight into this disease-associated phosphatase...
  16. ncbi Atomic structure of bacteriophage Sf6 tail needle knob
    Anshul Bhardwaj
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    J Biol Chem 286:30867-77. 2011
    ..In analogy to P22 gp26, we suggest the tail needle of phage Sf6 is ejected through the bacterial cell envelope during infection and its C-terminal knob is threaded through peptidoglycan pores formed by glycan strands...
  17. ncbi Dimeric quaternary structure of the prototypical dual specificity phosphatase VH1
    Adem C Koksal
    Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, Syracuse, New York 13210, USA
    J Biol Chem 284:10129-37. 2009
    ..We propose that the dimeric quaternary structure of VH1 is essential for specific recognition of activated STAT1, which prevents its nuclear translocation, thus blocking interferon-gamma signal transduction and antiviral response...

Research Grants6

  1. Molecular Basis for Recognition and Nuclear Import of Non-classical NLS Cargos
    Gino Cingolani; Fiscal Year: 2007
    ..Carry out a quantitative structural and functional analysis of HIV-1 Rev-NLS 3. Define the molecular basis for the recognition of STAT1 "dinner-specific" NLS by importin a-5. ..
  2. Molecular Basis for Recognition and Nuclear Import of Non-classical NLS Cargos
    Gino Cingolani; Fiscal Year: 2010
    ..Carry out a quantitative structural and functional analysis of HIV-1 Rev-NLS 3. Define the molecular basis for the recognition of STAT1 "dinner-specific"NLS by importin a-5. ..