T M Block

Summary

Affiliation: Thomas Jefferson University
Country: USA

Publications

  1. pmc A proteomic approach for the discovery of early detection markers of hepatocellular carcinoma
    L F Steel
    Department of Biochemistry and Molecular Pharmacology, Jefferson Center for Biomedical Research, Thomas Jefferson University, Doylestown, PA 18901, USA
    Dis Markers 17:179-89. 2001
  2. ncbi request reprint Molecular viral oncology of hepatocellular carcinoma
    Timothy M Block
    Department of Molecular Pharmacology and Biochemistry, Jefferson Center for Biomedical Research of Thomas Jefferson University, 700 East Butler Ave, Doylestown, PA 18901, USA
    Oncogene 22:5093-107. 2003
  3. ncbi request reprint Iminosugars as possible broad spectrum anti hepatitis virus agents: the glucovirs and alkovirs
    T M Block
    Department of Biochemistry and Molecular Pharmacology, Thomas Jefferson University, Doylestown, PA, USA
    Antivir Chem Chemother 12:317-25. 2001
  4. ncbi request reprint Inhibition of hepatitis B virus DNA replication by imino sugars without the inhibition of the DNA polymerase: therapeutic implications
    A Mehta
    Department of Biochemistry and Molecular Pharmacology, The Jefferson Center, Jefferson Medical College, Doylestown, PA 18901, USA
    Hepatology 33:1488-95. 2001
  5. doi request reprint Is chronic hepatitis B being undertreated in the United States?
    C Cohen
    Hepatitis B Foundation, Doylestown, PA 18902, USA
    J Viral Hepat 18:377-83. 2011
  6. ncbi request reprint Hepatitis B virus MHBs antigen is selectively sensitive to glucosidase-mediated processing in the endoplasmic reticulum
    X Lu
    Department of Biochemistry and Molecular Pharmacology, The Jefferson Center of Thomas Jefferson University, Doylestown, Pennsylvania, USA
    DNA Cell Biol 20:647-56. 2001
  7. ncbi request reprint Assays for glucosidase inhibitors with potential antiviral activities: secreted alkaline phosphatase as a surrogate marker
    Pamela A Norton
    Jefferson Center for Biomedical Research, Department of Biochemistry and Molecular Pharmacology, Thomas Jefferson University, Doylestown, PA 18901, USA
    J Virol Methods 124:167-72. 2005
  8. ncbi request reprint Discovery of small molecule inhibitors of West Nile virus using a high-throughput sub-genomic replicon screen
    Baohua Gu
    Drexel Institute for Biotechnology and Virology Research, Drexel University, College of Medicine, Doylestown, PA 18901, USA
    Antiviral Res 70:39-50. 2006
  9. ncbi request reprint Hepatitis B virus e antigen production is dependent upon covalently closed circular (ccc) DNA in HepAD38 cell cultures and may serve as a cccDNA surrogate in antiviral screening assays
    Tianlun Zhou
    Institute for Hepatitis Virus Research, Hepatitis B Foundation, 700 East Butler Avenue, Doylestown, PA 18901, USA
    Antiviral Res 72:116-24. 2006
  10. ncbi request reprint alpha-Glucosidase inhibitors have a prolonged antiviral effect against hepatitis B virus through the sustained inhibition of the large and middle envelope glycoproteins
    Ender Simsek
    Department of Biochemistry and Molecular Pharmacology, Thomas Jefferson University College of Medicine, Philadelphia, PA, USA
    Antivir Chem Chemother 17:259-67. 2006

Collaborators

Detail Information

Publications41

  1. pmc A proteomic approach for the discovery of early detection markers of hepatocellular carcinoma
    L F Steel
    Department of Biochemistry and Molecular Pharmacology, Jefferson Center for Biomedical Research, Thomas Jefferson University, Doylestown, PA 18901, USA
    Dis Markers 17:179-89. 2001
    ..Information may also be gleaned about the pathobiology of the disease process...
  2. ncbi request reprint Molecular viral oncology of hepatocellular carcinoma
    Timothy M Block
    Department of Molecular Pharmacology and Biochemistry, Jefferson Center for Biomedical Research of Thomas Jefferson University, 700 East Butler Ave, Doylestown, PA 18901, USA
    Oncogene 22:5093-107. 2003
    ..Viral functions may also play a more direct role in mediating oncogenesis. This review considers the molecular and cellular mechanisms involved in primary hepatocellular carcinoma, using a viral perspective...
  3. ncbi request reprint Iminosugars as possible broad spectrum anti hepatitis virus agents: the glucovirs and alkovirs
    T M Block
    Department of Biochemistry and Molecular Pharmacology, Thomas Jefferson University, Doylestown, PA, USA
    Antivir Chem Chemother 12:317-25. 2001
    ..These compounds are called 'alkovirs' and their mechanism of action is unknown. This review considers the rationale of the glucovir and alkovir approach to the treatment of hepatitis B and C...
  4. ncbi request reprint Inhibition of hepatitis B virus DNA replication by imino sugars without the inhibition of the DNA polymerase: therapeutic implications
    A Mehta
    Department of Biochemistry and Molecular Pharmacology, The Jefferson Center, Jefferson Medical College, Doylestown, PA 18901, USA
    Hepatology 33:1488-95. 2001
    ..The data suggest that N-nonyl-DGJ exerts its antiviral action at a point before viral envelopment and may prevent the proper encapsidation of the HBV pregenomic RNA...
  5. doi request reprint Is chronic hepatitis B being undertreated in the United States?
    C Cohen
    Hepatitis B Foundation, Doylestown, PA 18902, USA
    J Viral Hepat 18:377-83. 2011
    ....
  6. ncbi request reprint Hepatitis B virus MHBs antigen is selectively sensitive to glucosidase-mediated processing in the endoplasmic reticulum
    X Lu
    Department of Biochemistry and Molecular Pharmacology, The Jefferson Center of Thomas Jefferson University, Doylestown, Pennsylvania, USA
    DNA Cell Biol 20:647-56. 2001
    ..The reasons the MHBs polypeptide, but not SHBs, is so sensitive to glucosidase processing are discussed...
  7. ncbi request reprint Assays for glucosidase inhibitors with potential antiviral activities: secreted alkaline phosphatase as a surrogate marker
    Pamela A Norton
    Jefferson Center for Biomedical Research, Department of Biochemistry and Molecular Pharmacology, Thomas Jefferson University, Doylestown, PA 18901, USA
    J Virol Methods 124:167-72. 2005
    ..Thus, measuring SEAP secretion may be another method for evaluating glucosidase inhibition. In addition, this finding has important implications for the use of a SEAP reporter in screens of potential antiviral agents...
  8. ncbi request reprint Discovery of small molecule inhibitors of West Nile virus using a high-throughput sub-genomic replicon screen
    Baohua Gu
    Drexel Institute for Biotechnology and Virology Research, Drexel University, College of Medicine, Doylestown, PA 18901, USA
    Antiviral Res 70:39-50. 2006
    ..These compounds should be valuable for developing anti-WNV therapeutic drugs as well as research tools to study the mechanism of WNV replication...
  9. ncbi request reprint Hepatitis B virus e antigen production is dependent upon covalently closed circular (ccc) DNA in HepAD38 cell cultures and may serve as a cccDNA surrogate in antiviral screening assays
    Tianlun Zhou
    Institute for Hepatitis Virus Research, Hepatitis B Foundation, 700 East Butler Avenue, Doylestown, PA 18901, USA
    Antiviral Res 72:116-24. 2006
    ..Therefore, the secretion of HBeAg by HepAD38 cells could potentially serve as a convenient reporter for the high throughput screening of novel antiviral drugs targeting HBV cccDNA...
  10. ncbi request reprint alpha-Glucosidase inhibitors have a prolonged antiviral effect against hepatitis B virus through the sustained inhibition of the large and middle envelope glycoproteins
    Ender Simsek
    Department of Biochemistry and Molecular Pharmacology, Thomas Jefferson University College of Medicine, Philadelphia, PA, USA
    Antivir Chem Chemother 17:259-67. 2006
    ..The implications of the prolonged antiviral effect against HBV and the use of glucosidase inhibitors as antiviral agents are discussed...
  11. ncbi request reprint Proteomic analysis of serum associated fucosylated glycoproteins in the development of primary hepatocellular carcinoma
    Mary Ann Comunale
    Drexel Institute for Biotechnology and Virology Research, Department of Microbiology and Immunology, Drexel University College of Medicine, Doylestown, Pennsylvania 18901, USA
    J Proteome Res 5:308-15. 2006
    ..In total, 19 proteins were found to be hyperfucosylated in cancer. The potential of these proteins as biomarkers of cancer is discussed...
  12. pmc Characterization of the intracellular deproteinized relaxed circular DNA of hepatitis B virus: an intermediate of covalently closed circular DNA formation
    Haitao Guo
    Drexel Institute for Biotechnology and Virology Research, Department of Microbiology and Immunology, Drexel University College of Medicine, Doylestown, PA 18902, USA
    J Virol 81:12472-84. 2007
    ....
  13. ncbi request reprint Herpes simplex virus type 1 ICP4 deletion mutant virus d120 infection failed to induce apoptosis in nerve growth factor-differentiated PC12 cells
    Benjamas Aiamkitsumrit
    Drexel Institute for Biotechnology and Virology Research and Department of Microbiology and Immunology, College of Medicine, Drexel University, Doylestown, Pennsylvania, USA
    J Neurovirol 13:305-14. 2007
    ..The possible mechanisms involved in the failure of d120 to induce apoptosis in neuronal-like NGF-differentiated PC12 cells are discussed...
  14. ncbi request reprint Alpha interferon-induced antiviral response noncytolytically reduces replication defective adenovirus DNA in MDBK cells
    Ju Tao Guo
    Drexel Institute for Biotechnology and Virology Research, Department of Microbiology and Immunology, Drexel University College of Medicine, Doylestown, PA 18902, USA
    Antiviral Res 76:232-40. 2007
    ..Our data suggest that IFN-alpha-induced antiviral program is able to discriminate host cellular DNA from episomal viral DNA and might represent a novel pathway of interferon mediate innate defense against DNA virus infections...
  15. pmc Molecular virology of hepatitis B virus for clinicians
    Timothy M Block
    Department of Microbiology and Immunology, Drexel University College of Medicine, 3805 Old Easton Road, Doylestown, PA 18902, USA
    Clin Liver Dis 11:685-706, vii. 2007
    ..The way in which these steps may influence the natural history of viral pathogenesis, as well as the effectiveness of interventions, receives special consideration...
  16. pmc Regulation of hepatitis B virus replication by the phosphatidylinositol 3-kinase-akt signal transduction pathway
    Haitao Guo
    Drexel Institute for Biotechnology and Virology Research, Department of Microbiology and Immunology, Drexel University College of Medicine, 3805 Old Easton Road, Doylestown, PA 18902, USA
    J Virol 81:10072-80. 2007
    ....
  17. pmc Identification of three interferon-inducible cellular enzymes that inhibit the replication of hepatitis C virus
    Dong Jiang
    Drexel Institute for Biotechnology and Virology Research, Department of Microbiology and Immunology, Drexel University College of Medicine, 3805 Old Easton Road, Doylestown, PA 18902, USA
    J Virol 82:1665-78. 2008
    ..Our findings suggest that viperin represents a novel antiviral pathway that works together with other antiviral proteins, such as ISG20 and PKR, to mediate the IFN response against HCV infection...
  18. pmc Liver-specific microRNA miR-122 enhances the replication of hepatitis C virus in nonhepatic cells
    Jinhong Chang
    Drexel Institute for Biotechnology and Virology Research, Department of Microbiology and Immunology, Drexel University College of Medicine, 3805 Old Easton Road, Doylestown, PA 18902, USA
    J Virol 82:8215-23. 2008
    ..Therefore, we conclude that although miR-122 is not absolutely required, it greatly enhances HCV replication in nonhepatic cells...
  19. ncbi request reprint Glyco- and peptidomimetics from three-component JoulliƩ-Ugi coupling show selective antiviral activity
    Timothy M Chapman
    Chemistry Research Laboratory, University of Oxford, Mansfield Road, Oxford UK, OX1 3TA
    J Am Chem Soc 127:506-7. 2005
    ..An observed selectivity for this HCV model over hepatitis B virus and remarkably low toxicity suggest a novel mode of action...
  20. pmc Hepatitis B virus-mediated changes of apolipoprotein mRNA abundance in cultured hepatoma cells
    Pamela A Norton
    Jefferson Center for Biomedical Research and Department of Biochemistry and Molecular Pharmacology, Thomas Jefferson University, Doylestown, Pennsylvania, USA
    J Virol 77:5503-6. 2003
    ..Analysis of a third line containing an inducible viral genome implicated viral pregenomic RNA in apolipoprotein mRNA reduction. We conclude that HBV alters infected cells despite the absence of overt cytopathogenicity...
  21. ncbi request reprint Imino sugars that are less toxic but more potent as antivirals, in vitro, compared with N-n-nonyl DNJ
    Anand Mehta
    Department of Biochemistry and Molecular Pharmacology, The Jefferson Center of Thomas Jefferson University, Doylestown, PA, USA
    Antivir Chem Chemother 13:299-304. 2002
    ..The results of this antiviral survey and the implications for the mechanism of action and ultimate therapeutic potential of the DNJ-based imino sugars is provided and discussed...
  22. pmc Structure-activity relationship of a new class of anti-hepatitis B virus agents
    Anand Mehta
    Department of Biochemistry and Molecular Pharmacology, The Jefferson Center, Jefferson Medical College, Doylestown, Pennsylvania 18901 2697, USA
    Antimicrob Agents Chemother 46:4004-8. 2002
    ..This work suggests that the antiviral activity of the alkovirs requires an alkyl chain length of at least eight carbons but that the galactose-based head group can be modified with little or no loss in activity...
  23. ncbi request reprint A strategy for the comparative analysis of serum proteomes for the discovery of biomarkers for hepatocellular carcinoma
    Laura F Steel
    Department of Biochemistry and Molecular Pharmacology, Thomas Jefferson University, Jefferson Center for Biomedical Research, Doylestown, PA 18901, USA
    Proteomics 3:601-9. 2003
    ..These preliminary studies suggest that a proteomic methodology can be used for the identification of serum biomarkers for HCC and other liver disease...
  24. pmc Replication of a cytopathic strain of bovine viral diarrhea virus activates PERK and induces endoplasmic reticulum stress-mediated apoptosis of MDBK cells
    Robert Jordan
    Department of Biochemistry and Molecular Pharmacology, The Jefferson Center for Biomedical Research, Thomas Jefferson University, Doylestown, Pennsylvania 18901, USA
    J Virol 76:9588-99. 2002
    ....
  25. ncbi request reprint The combination of interferon alpha-2b and n-butyl deoxynojirimycin has a greater than additive antiviral effect upon production of infectious bovine viral diarrhea virus (BVDV) in vitro: implications for hepatitis C virus (HCV) therapy
    Serguey Ouzounov
    Department of Biochemistry and Molecular Pharmacology, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Antiviral Res 55:425-35. 2002
    ..These data are consistent with an interpretation that glucosidase inhibitors and IFN have a synergistic antiviral effect in tissue culture. The relevance of these finding to treatment of HCV infection is discussed...
  26. pmc The alkylated imino sugar, n-(n-Nonyl)-deoxygalactonojirimycin, reduces the amount of hepatitis B virus nucleocapsid in tissue culture
    Xuanyong Lu
    Biochemistry and Molecular Pharmacology Department, Jefferson Center for Bio Medical Research and Agricultural Medicine, Thomas Jefferson University, Doylestown, Pennsylvania 18901, USA
    J Virol 77:11933-40. 2003
    ..The similarities and differences between this alkylated imino sugar and these other mediators are discussed...
  27. ncbi request reprint Comparative proteomic analysis of de-N-glycosylated serum from hepatitis B carriers reveals polypeptides that correlate with disease status
    Mary Ann Comunale
    Department of Biochemistry and Molecular Pharmacology, Thomas Jefferson University, The Jefferson Center, Doylestown, PA 18901, USA
    Proteomics 4:826-38. 2004
    ....
  28. pmc alpha-Galactosylceramide and novel synthetic glycolipids directly induce the innate host defense pathway and have direct activity against hepatitis B and C viruses
    Anand S Mehta
    Department of Biochemistry, The Jefferson Center, Jefferson Medical College, 700 E Butler Ave, Doylestown, PA 18901, USA
    Antimicrob Agents Chemother 48:2085-90. 2004
    ..To exploit this activity, we have developed a new class of smaller, orally available glycolipids that also induce the innate host defense pathway and have direct activity against HBV and hepatitis C virus...
  29. ncbi request reprint Herpes simplex virus type 1 infection prevents detachment of nerve growth factor-differentiated PC12 cells in culture
    Michael J Moxley
    Jefferson Center for Biomedical Research of Thomas Jefferson University, Department of Biochemistry and Molecular Pharmacology, 700 E Butler Avenue, Doylestown, PA 18901 2697, USA
    J Gen Virol 83:1591-600. 2002
    ..These findings indicate that de novo viral gene synthesis mediates changes to the host NGF-differentiated PC12 cells, which results in prevention of detachment...
  30. ncbi request reprint Inhibition of host ER glucosidase activity prevents Golgi processing of virion-associated bovine viral diarrhea virus E2 glycoproteins and reduces infectivity of secreted virions
    Robert Jordan
    Department of Biochemistry and Molecular Pharmacology, Thomas Jefferson University, Doylestown, Pennsylvania, 18901, USA
    Virology 295:10-9. 2002
    ....
  31. ncbi request reprint Stability and circularization of herpes simplex virus type 1 genomes in quiescently infected PC12 cultures
    Ying Hsiu Su
    Jefferson Center for Biomedical Research of Thomas Jefferson University, 700 E Butler Ave, Doylestown, PA 18901 2697, USA
    J Gen Virol 83:2943-50. 2002
    ..The relevance to the virus life-cycle in neurones in vivo is discussed...
  32. pmc Increased levels of galactose-deficient anti-Gal immunoglobulin G in the sera of hepatitis C virus-infected individuals with fibrosis and cirrhosis
    Anand S Mehta
    Drexel University College of Medicine and Department of Microbiology and Immunology and Drexel Institute for Biotechnology and Virology, Doylestown, PA 18901, USA
    J Virol 82:1259-70. 2008
    ..The reason for the alteration in the glycosylation of anti-Gal IgG is currently unclear but may be related to the natural history of the disease and may be useful in the noninvasive detection of fibrosis and cirrhosis...
  33. ncbi request reprint The degree of readiness of selected biomarkers for the early detection of hepatocellular carcinoma: notes from a recent workshop
    Timothy M Block
    Drexel Institute for Biotechnology and Virology Research, Drexel University College of Medicine, Doylestown, PA 18902, USA
    Cancer Biomark 4:19-33. 2008
    ..It is emphasized that only a selected set of biomarkers was considered; thus, this review is not comprehensive and not intended to review all candidate HCC biomarkers...
  34. pmc Use of targeted glycoproteomics to identify serum glycoproteins that correlate with liver cancer in woodchucks and humans
    Timothy M Block
    Drexel Institute for Biotechnology and Virology Research, Drexel University, Doylestown, PA 18901, USA
    Proc Natl Acad Sci U S A 102:779-84. 2005
    ..The potential of this technology for biomarker discovery and the implications of increased levels of GP73 in liver cancer are discussed...
  35. ncbi request reprint The stability of herpes simplex virus type I genomes in infected Vero cells undergoing viral induced apoptosis
    Ying Hsiu Su
    Department of Microbiology and Immunology, College of Medicine, Drexel University, Doylestown, Pennsylvania 18901 2697, USA
    J Neurovirol 12:375-86. 2006
    ..The possibility that the genome structure and replication compartment formation provide protection to the HSV-1 genome from degradation is discussed...
  36. pmc Evidence that the immediate-early gene product ICP4 is necessary for the genome of the herpes simplex virus type 1 ICP4 deletion mutant strain d120 to circularize in infected cells
    Ying Hsiu Su
    Department of Microbiology and Immunology, Drexel Institute for Biotechnology and Virology Research, College of Medicine, Drexel University, 3805 Old Easton Road, Doylestown, PA 18901 2697, USA
    J Virol 80:11589-97. 2006
    ..Collectively, these data suggest that ICP4 protein has an important role in mediating the endless formation of the HSV-1 genome upon infection and that this function can be provided in trans...
  37. ncbi request reprint Does rapid oligomerization of hepatitis B envelope proteins play a role in resistance to proteasome degradation and enhance chronicity?
    Timothy M Block
    Department of Microbiology and Immunology, Drexel Institute for Biotechnology and Virology Research, Drexel University College of Medicine, Doylestown, Pennsylvania 18901, USA
    DNA Cell Biol 25:165-70. 2006
    ..The concept offers an explanation for the nearly unidirectional and rapid kinetics whereby HBV proteins form multimers and generate a surplus of viral structures that have not been thought to serve any useful structural purpose...
  38. ncbi request reprint GP73, a resident Golgi glycoprotein, is a novel serum marker for hepatocellular carcinoma
    Jorge A Marrero
    Division of Gastroenterology, University of Michigan Medical Center, 3912 Taubman Center, Ann Arbor, MI 48109 0362, USA
    J Hepatol 43:1007-12. 2005
    ..Golgi protein-73 (GP73) is up-regulated in hepatocellular carcinoma (HCC). The aims of this study were to determine if GP73 is detected in the serum, and to establish the sensitivity and specificity of serum GP73 for diagnosing HCC...
  39. ncbi request reprint SELDI-TOF MS profiling of serum for detection of the progression of chronic hepatitis C to hepatocellular carcinoma
    E Ellen Schwegler
    Department of Microbiology and Molecular Cell Biology, Eastern Virginia Medical School, Norfolk, VA, USA
    Hepatology 41:634-42. 2005
    ..In conclusion, SELDI-TOF MS serum profiling is able to distinguish HCC from liver disease before cirrhosis as well as cirrhosis, especially in patients with HCV infection compared with other etiologies...
  40. pmc Construction of a herpes simplex virus type 1 mutant with only a three-nucleotide change in the branchpoint region of the latency-associated transcript (LAT) and the stability of its two-kilobase LAT intron
    Alan K Ng
    Department of Biochemistry and Molecular Pharmacology, Jefferson Center for Biomedical Research, Thomas Jefferson University, 700 E Butler Avenue, Doylestown, PA 18901 2697, USA
    J Virol 78:12097-106. 2004
    ..However, the ability of mutant Sy2 to reactivate from trigeminal ganglia (TG) derived from latently infected mice was indistinguishable from that of wild-type virus, as assayed in the mouse TG explant reactivation system...
  41. ncbi request reprint N-linked glycosylation of the liver cancer biomarker GP73
    Pamela A Norton
    Department of Microbiology and Immunology, Drexel University College of Medicine, Doylestown, Pennsylvania 48902, USA
    J Cell Biochem 104:136-49. 2008
    ....