Pavel V Bondarenko

Summary

Affiliation: Thermo Finnigan
Country: USA

Publications

  1. ncbi request reprint Identification and relative quantitation of protein mixtures by enzymatic digestion followed by capillary reversed-phase liquid chromatography-tandem mass spectrometry
    Pavel V Bondarenko
    ThermoFinnigan, San Jose, California 95134 1991, USA
    Anal Chem 74:4741-9. 2002
  2. ncbi request reprint Identification of N-linked oligosaccharides of rat insulin-like growth factor binding protein-4
    Dirk Chelius
    Thermo Finnigan, Proteomics Division, 355 River Oaks Parkway, San Jose, CA 95134, USA
    Growth Horm IGF Res 12:169-77. 2002
  3. ncbi request reprint Quantitative profiling of proteins in complex mixtures using liquid chromatography and mass spectrometry
    Dirk Chelius
    Thermo Finnigan, 355 River Oaks Parkway, San Jose, California 95134, USA
    J Proteome Res 1:317-23. 2002
  4. ncbi request reprint Reversed-phase liquid chromatography of immunoglobulin G molecules and their fragments with the diphenyl column
    Da Ren
    Department of Pharmaceutics, Amgen Inc, One Amgen Center Drive, Thousand Oaks, CA 91320, USA
    J Chromatogr A 1175:63-8. 2007
  5. ncbi request reprint The LC/MS analysis of glycation of IgG molecules in sucrose containing formulations
    Himanshu S Gadgil
    Department of Pharmaceutics, Amgen Inc, 1201 Amgen Court West, Seattle, Washington 99119 3105, USA
    J Pharm Sci 96:2607-21. 2007
  6. ncbi request reprint Screening and sequencing of glycated proteins by neutral loss scan LC/MS/MS method
    Himanshu S Gadgil
    Department of Pharmaceutics, Amgen, Inc, Thousand Oaks, California 91320, USA
    Anal Chem 79:5991-9. 2007
  7. ncbi request reprint 18O labeling method for identification and quantification of succinimide in proteins
    Gang Xiao
    Department of Pharmaceutics, Amgen, Inc, Thousand Oaks, California 91320, USA
    Anal Chem 79:2714-21. 2007
  8. ncbi request reprint Identification of cysteinylation of a free cysteine in the Fab region of a recombinant monoclonal IgG1 antibody using Lys-C limited proteolysis coupled with LC/MS analysis
    Himanshu S Gadgil
    Amgen Inc, Thousand Oaks, CA 91320, USA
    Anal Biochem 355:165-74. 2006
  9. ncbi request reprint Improving mass accuracy of high performance liquid chromatography/electrospray ionization time-of-flight mass spectrometry of intact antibodies
    Himanshu S Gadgil
    Department of Pharmaceutics, Amgen, Thousand Oaks, California 91320, USA
    J Am Soc Mass Spectrom 17:867-72. 2006
  10. ncbi request reprint Optimization of a reversed-phase high-performance liquid chromatography/mass spectrometry method for characterizing recombinant antibody heterogeneity and stability
    Thomas M Dillon
    Pharmaceutics Department, Amgen Inc, One Amgen Center Dr, Thousand Oaks, CA 91320, USA
    J Chromatogr A 1120:112-20. 2006

Collaborators

  • Himanshu S Gadgil
  • Dirk Chelius
  • Gary D Pipes
  • Thomas M Dillon
  • Douglas S Rehder
  • Da Ren
  • Gang Xiao
  • David N Brems
  • Jaby Jacob
  • David M Hambly
  • Grace C Chu
  • Michael J Treuheit
  • Margaret Speed Ricci
  • Gerd R Kleemann

Detail Information

Publications12

  1. ncbi request reprint Identification and relative quantitation of protein mixtures by enzymatic digestion followed by capillary reversed-phase liquid chromatography-tandem mass spectrometry
    Pavel V Bondarenko
    ThermoFinnigan, San Jose, California 95134 1991, USA
    Anal Chem 74:4741-9. 2002
    ..In the presented method, chemical derivatization steps are not needed to create an internal standard, as in isotope-coded affinity tag or similar methods...
  2. ncbi request reprint Identification of N-linked oligosaccharides of rat insulin-like growth factor binding protein-4
    Dirk Chelius
    Thermo Finnigan, Proteomics Division, 355 River Oaks Parkway, San Jose, CA 95134, USA
    Growth Horm IGF Res 12:169-77. 2002
    ..Identified glycans were biantennary and differ in the number of sialic acid terminal residues and/or core modification with fucose...
  3. ncbi request reprint Quantitative profiling of proteins in complex mixtures using liquid chromatography and mass spectrometry
    Dirk Chelius
    Thermo Finnigan, 355 River Oaks Parkway, San Jose, California 95134, USA
    J Proteome Res 1:317-23. 2002
    ..We describe a new method combining protein identification with accurate profiling of individual proteins. This approach should provide a widely applicable means to compare global protein expression in biological samples...
  4. ncbi request reprint Reversed-phase liquid chromatography of immunoglobulin G molecules and their fragments with the diphenyl column
    Da Ren
    Department of Pharmaceutics, Amgen Inc, One Amgen Center Drive, Thousand Oaks, CA 91320, USA
    J Chromatogr A 1175:63-8. 2007
    ..pi-pi interactions and the large contact surface resulted in improved resolution of IgGs and their fragments on the diphenyl column...
  5. ncbi request reprint The LC/MS analysis of glycation of IgG molecules in sucrose containing formulations
    Himanshu S Gadgil
    Department of Pharmaceutics, Amgen Inc, 1201 Amgen Court West, Seattle, Washington 99119 3105, USA
    J Pharm Sci 96:2607-21. 2007
    ..The amino terminal end of the light chain was also shown to contain glycation. The surface accessibility of the lysine side chain could influence its susceptibility to glycation...
  6. ncbi request reprint Screening and sequencing of glycated proteins by neutral loss scan LC/MS/MS method
    Himanshu S Gadgil
    Department of Pharmaceutics, Amgen, Inc, Thousand Oaks, California 91320, USA
    Anal Chem 79:5991-9. 2007
    ..Using this method, we were able to identify that 31 lysine residues were partially glycated from the glycated HSA sample, which has a total of 59 lysine residues...
  7. ncbi request reprint 18O labeling method for identification and quantification of succinimide in proteins
    Gang Xiao
    Department of Pharmaceutics, Amgen, Inc, Thousand Oaks, California 91320, USA
    Anal Chem 79:2714-21. 2007
    ..This finding indicates that protein structure plays an important role in the kinetics of succinimide hydrolysis as well as in the generation of the hydrolysis products isoaspartic acid and aspartic acid...
  8. ncbi request reprint Identification of cysteinylation of a free cysteine in the Fab region of a recombinant monoclonal IgG1 antibody using Lys-C limited proteolysis coupled with LC/MS analysis
    Himanshu S Gadgil
    Amgen Inc, Thousand Oaks, CA 91320, USA
    Anal Biochem 355:165-74. 2006
    ..The cysteinylation observed after limited proteolysis was confirmed by peptide mapping coupled with tandem mass spectrometry analysis...
  9. ncbi request reprint Improving mass accuracy of high performance liquid chromatography/electrospray ionization time-of-flight mass spectrometry of intact antibodies
    Himanshu S Gadgil
    Department of Pharmaceutics, Amgen, Thousand Oaks, California 91320, USA
    J Am Soc Mass Spectrom 17:867-72. 2006
    ..Mass accuracy in measuring different antibodies was below 30 ppm (+/-4 Da). The accurate mass measurement can be an effective tool for monitoring chemical degradations in therapeutic antibodies...
  10. ncbi request reprint Optimization of a reversed-phase high-performance liquid chromatography/mass spectrometry method for characterizing recombinant antibody heterogeneity and stability
    Thomas M Dillon
    Pharmaceutics Department, Amgen Inc, One Amgen Center Dr, Thousand Oaks, CA 91320, USA
    J Chromatogr A 1120:112-20. 2006
    ..Mass spectral characterization data and reduction data suggested that the heterogeneity is disulfide related. This reversed-phase LC/MS method represents a key advancement in monitoring intact MAb production and stability...
  11. ncbi request reprint Reversed-phase liquid chromatography/mass spectrometry analysis of reduced monoclonal antibodies in pharmaceutics
    Douglas S Rehder
    Amgen, Department of Pharmaceutics, One Amgen Center Drive, MS 8 1 C, Thousand Oaks, CA 91320, USA
    J Chromatogr A 1102:164-75. 2006
    ..Deconvoluted electrospray ionization mass spectrum of the heavy chain revealed the glycosylation profile of a single N-linked sugar including a-, mono-, and di-galactosylated biantennary glycans and a 5-mannose sugar form...
  12. doi request reprint Automated tryptic digestion procedure for HPLC/MS/MS peptide mapping of immunoglobulin gamma antibodies in pharmaceutics
    Dirk Chelius
    Amgen, Process and Product Development One Amgen Center Drive, Thousand Oaks, CA 91320, USA
    J Pharm Biomed Anal 47:285-94. 2008
    ....