Qinghai Zhang

Summary

Affiliation: The Scripps Research Institute
Country: USA

Publications

  1. ncbi request reprint Designing facial amphiphiles for the stabilization of integral membrane proteins
    Qinghai Zhang
    Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Angew Chem Int Ed Engl 46:7023-5. 2007
  2. pmc New amphiphiles for membrane protein structural biology
    Qinghai Zhang
    Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Methods 55:318-23. 2011
  3. pmc Structural analysis of mammalian cytochrome P450 2B4 covalently bound to the mechanism-based inactivator tert-butylphenylacetylene: insight into partial enzymatic activity
    Sean C Gay
    Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, La Jolla, California 92093, USA
    Biochemistry 50:4903-11. 2011
  4. pmc A structural snapshot of CYP2B4 in complex with paroxetine provides insights into ligand binding and clusters of conformational states
    Manish B Shah
    Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, 9500 Gilman Drive, Mail Code 0703, La Jolla, CA 92093 0703, USA
    J Pharmacol Exp Ther 346:113-20. 2013
  5. pmc Structures of cytochrome P450 2B4 complexed with the antiplatelet drugs ticlopidine and clopidogrel
    Sean C Gay
    Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, La Jolla, California 92093, USA
    Biochemistry 49:8709-20. 2010
  6. pmc Micro-coil NMR to monitor optimization of the reconstitution conditions for the integral membrane protein OmpW in detergent micelles
    Pawel Stanczak
    Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    J Biomol NMR 54:129-33. 2012
  7. pmc Structures of cytochrome P450 2B6 bound to 4-benzylpyridine and 4-(4-nitrobenzyl)pyridine: insight into inhibitor binding and rearrangement of active site side chains
    Manish B Shah
    Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, La Jolla, CA 92093 0703, USA
    Mol Pharmacol 80:1047-55. 2011
  8. pmc Plasticity of cytochrome P450 2B4 as investigated by hydrogen-deuterium exchange mass spectrometry and X-ray crystallography
    P Ross Wilderman
    Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, California 92093, USA
    J Biol Chem 285:38602-11. 2010
  9. pmc Steroid-based facial amphiphiles for stabilization and crystallization of membrane proteins
    Sung Chang Lee
    Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 110:E1203-11. 2013
  10. pmc Investigation by site-directed mutagenesis of the role of cytochrome P450 2B4 non-active-site residues in protein-ligand interactions based on crystal structures of the ligand-bound enzyme
    P Ross Wilderman
    Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, CA 92093, USA
    FEBS J 279:1607-20. 2012

Collaborators

Detail Information

Publications19

  1. ncbi request reprint Designing facial amphiphiles for the stabilization of integral membrane proteins
    Qinghai Zhang
    Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Angew Chem Int Ed Engl 46:7023-5. 2007
  2. pmc New amphiphiles for membrane protein structural biology
    Qinghai Zhang
    Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Methods 55:318-23. 2011
    ..Literature examples and some new developments are selected here as a framework for discussing desirable properties of new amphiphiles for MP structural biology...
  3. pmc Structural analysis of mammalian cytochrome P450 2B4 covalently bound to the mechanism-based inactivator tert-butylphenylacetylene: insight into partial enzymatic activity
    Sean C Gay
    Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, La Jolla, California 92093, USA
    Biochemistry 50:4903-11. 2011
    ..However, docking experiments using this open form show that tBPA is able to rotate upward to give testosterone and 7-ethoxy-4-trifluoromethylcoumarin access to the heme, which could explain the previously observed partial activity...
  4. pmc A structural snapshot of CYP2B4 in complex with paroxetine provides insights into ligand binding and clusters of conformational states
    Manish B Shah
    Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, 9500 Gilman Drive, Mail Code 0703, La Jolla, CA 92093 0703, USA
    J Pharmacol Exp Ther 346:113-20. 2013
    ..Overall, the results from the comparison of all 16 structures of CYP2B4 demonstrate a cluster of protein conformations that were observed in the presence or absence of various ligands...
  5. pmc Structures of cytochrome P450 2B4 complexed with the antiplatelet drugs ticlopidine and clopidogrel
    Sean C Gay
    Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, La Jolla, California 92093, USA
    Biochemistry 49:8709-20. 2010
    ..Adjusting both protein conformation and ligand orientation in the active site gives 2B4 the flexibility to bind to the widest range of molecules, while also being energetically favorable...
  6. pmc Micro-coil NMR to monitor optimization of the reconstitution conditions for the integral membrane protein OmpW in detergent micelles
    Pawel Stanczak
    Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    J Biomol NMR 54:129-33. 2012
    ..Some emerging guidelines for reconstitution of β-barrel integral membrane proteins in structural biology are discussed...
  7. pmc Structures of cytochrome P450 2B6 bound to 4-benzylpyridine and 4-(4-nitrobenzyl)pyridine: insight into inhibitor binding and rearrangement of active site side chains
    Manish B Shah
    Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, La Jolla, CA 92093 0703, USA
    Mol Pharmacol 80:1047-55. 2011
    ....
  8. pmc Plasticity of cytochrome P450 2B4 as investigated by hydrogen-deuterium exchange mass spectrometry and X-ray crystallography
    P Ross Wilderman
    Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, California 92093, USA
    J Biol Chem 285:38602-11. 2010
    ..The results indicate that conformational changes observed in available crystal structures of the promiscuous xenobiotic metabolizing cytochrome P450 2B4 are consistent with its solution structural behavior...
  9. pmc Steroid-based facial amphiphiles for stabilization and crystallization of membrane proteins
    Sung Chang Lee
    Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 110:E1203-11. 2013
    ....
  10. pmc Investigation by site-directed mutagenesis of the role of cytochrome P450 2B4 non-active-site residues in protein-ligand interactions based on crystal structures of the ligand-bound enzyme
    P Ross Wilderman
    Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, CA 92093, USA
    FEBS J 279:1607-20. 2012
    ..The results indicate that small changes in the protein scaffold lead to large differences in solution behavior and enzyme function...
  11. pmc Design, synthesis, and properties of branch-chained maltoside detergents for stabilization and crystallization of integral membrane proteins: human connexin 26
    Wen Xu Hong
    Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Langmuir 26:8690-6. 2010
    ..We showed that the branch-chained maltosides with optimized micellar properties performed as well as or better than the straight-chained analogues and enabled crystallization in different space groups...
  12. pmc Structure of a cation-bound multidrug and toxic compound extrusion transporter
    Xiao He
    Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, CB105, La Jolla, California 92037, USA
    Nature 467:991-4. 2010
    ..This conformation probably represents a stage of the transport cycle with high affinity for monovalent cations and low affinity for substrates...
  13. pmc Engineered nanostructured β-sheet peptides protect membrane proteins
    Houchao Tao
    Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, California, USA
    Nat Methods 10:759-61. 2013
    ..strand peptides enabled clear visualization of flexible conformations in the highly dynamic ATP-binding cassette (ABC) transporter MsbA. ..
  14. pmc Microscale NMR screening of new detergents for membrane protein structural biology
    Qinghai Zhang
    Department of Molecular Biology, Department of Chemistry, Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    J Am Chem Soc 130:7357-63. 2008
    ....
  15. pmc Crystal structure of CYP24A1, a mitochondrial cytochrome P450 involved in vitamin D metabolism
    Andrew J Annalora
    Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    J Mol Biol 396:441-51. 2010
    ..Overall, the CYP24A1 structure provides the first template for understanding membrane insertion, substrate binding, and redox partner interaction in mitochondrial P450s...
  16. pmc Synthesis and properties of dodecyl trehaloside detergents for membrane protein studies
    Houchao Tao
    Department of Molecular Biology, The Scripps Research Institute, La Jolla, California 92037, United States
    Langmuir 28:11173-81. 2012
    ..Continuing development and investigations of trehaloside detergents are attractive, given their interesting and unique chemical-physical properties and potential interactions with membrane lipids...
  17. pmc Crystal structure of a cytochrome P450 2B6 genetic variant in complex with the inhibitor 4-(4-chlorophenyl)imidazole at 2.0-A resolution
    Sean C Gay
    Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, 9500 Gilman Dr 0703, La Jolla, CA 92093, USA
    Mol Pharmacol 77:529-38. 2010
    ..However, it is unknown whether 2B6 undergoes structural reorganization to accommodate bulkier molecules, as previously inferred from multiple P450 2B4 crystal structures...
  18. pmc Structure of P-glycoprotein reveals a molecular basis for poly-specific drug binding
    Stephen G Aller
    Department of Molecular Biology, Scripps Research Institute, 10550 North Torrey Pines Road, CB105, La Jolla, CA 92037, USA
    Science 323:1718-22. 2009
    ..The inward-facing conformation represents an initial stage of the transport cycle that is competent for drug binding...
  19. doi request reprint Synthesis of azole-enriched cyclic peptides by a clean solid-phase-based cyclization-cleavage strategy
    Houchao Tao
    Department of Integrative Structural and Computational Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, United States
    ACS Comb Sci 15:447-51. 2013
    ..This development is ideal for high throughput library synthesis and screening and will facilitate the ultimate discovery of novel azole cyclopeptides with potent biological activities. ..