Shang Tian Yang

Summary

Affiliation: The Ohio State University
Country: USA

Publications

  1. ncbi request reprint A fibrous-bed bioreactor for continuous production of monoclonal antibody by hybridoma
    Shang Tian Yang
    Department of Chemical Engineering, The Ohio State University, 140 West 19th Avenue, Columbus, Ohio 43210, USA
    Adv Biochem Eng Biotechnol 87:61-96. 2004
  2. ncbi request reprint Production of GFP and glucoamylase by recombinant Aspergillus niger: effects of fermentation conditions on fungal morphology and protein secretion
    Mylene Talabardon
    Department of Chemical and Biomolecular Engineering, The Ohio State University, 140 West 19th Avenue, Columbus, Ohio 43210, USA
    Biotechnol Prog 21:1389-400. 2005
  3. doi request reprint The future of microfluidic assays in drug development
    Yuan Wen
    The Ohio State University, Department of Chemical and Biomolecular Engineering, 140 West 19th Avenue, Columbus, Ohio 43210, USA 1 614 2926611 1 614 2923769
    Expert Opin Drug Discov 3:1237-53. 2008
  4. ncbi request reprint CELLutions--CHI's Second Annual Summit: The Future of Stem Cell Sciences. Third annual stem cell sources: targeting cell-based regenerative therapies. 20-23 August 2007, Boston, MA, USA
    Shang Tian Yang
    Ohio State University, College of Engineering, 321B Koffolt, 140 W Nineteenth Avenue, Columbus, OH 43210, USA
    IDrugs 10:696-8. 2007
  5. ncbi request reprint Microbioreactors for high-throughput cytotoxicity assays
    Shang Tian Yang
    The Ohio State University, Department of Chemical and Biomolecular Engineering, 140 West 19th Avenue, Columbus, OH 43210, USA
    Curr Opin Drug Discov Devel 11:111-27. 2008
  6. ncbi request reprint Production of galacto-oligosaccharides from lactose by Aspergillus oryzae beta-galactosidase immobilized on cotton cloth
    Nedim Albayrak
    Department of Chemical Engineering, The Ohio State University, 140 West 19th Avenue, Columbus, Ohio 43210, USA
    Biotechnol Bioeng 77:8-19. 2002
  7. ncbi request reprint Production of L(+)-lactic acid from glucose and starch by immobilized cells of Rhizopus oryzae in a rotating fibrous bed bioreactor
    Abdullatif Tay
    Department of Chemical Engineering, The Ohio State University 140 West 19th Avenue, Columbus, Ohio 43210, USA
    Biotechnol Bioeng 80:1-12. 2002
  8. ncbi request reprint Fabrication of well-defined PLGA scaffolds using novel microembossing and carbon dioxide bonding
    Yong Yang
    The Department of Chemical and Biomolecular Engineering, The Ohio State University, 140 West 19th Avenue, Columbus, OH 43210, USA
    Biomaterials 26:2585-94. 2005
  9. ncbi request reprint Effects of three-dimensional culturing on osteosarcoma cells grown in a fibrous matrix: analyses of cell morphology, cell cycle, and apoptosis
    Chunnuan Chen
    Department of Chemical Engineering, The Ohio State University, 140 West 19th Avenue, Columbus, Ohio 43210, USA
    Biotechnol Prog 19:1574-82. 2003
  10. ncbi request reprint Adaptation of Clostridium tyrobutyricum for enhanced tolerance to butyric acid in a fibrous-bed bioreactor
    Ying Zhu
    Department of Chemical Engineering, The Ohio State University, 140 West 19th Avenue, Columbus, Ohio 43210, USA
    Biotechnol Prog 19:365-72. 2003

Collaborators

Detail Information

Publications45

  1. ncbi request reprint A fibrous-bed bioreactor for continuous production of monoclonal antibody by hybridoma
    Shang Tian Yang
    Department of Chemical Engineering, The Ohio State University, 140 West 19th Avenue, Columbus, Ohio 43210, USA
    Adv Biochem Eng Biotechnol 87:61-96. 2004
    ....
  2. ncbi request reprint Production of GFP and glucoamylase by recombinant Aspergillus niger: effects of fermentation conditions on fungal morphology and protein secretion
    Mylene Talabardon
    Department of Chemical and Biomolecular Engineering, The Ohio State University, 140 West 19th Avenue, Columbus, Ohio 43210, USA
    Biotechnol Prog 21:1389-400. 2005
    ..The RFB bioreactor gave the best fermentation performance because of its ability to control the cell morphology that was amenable to efficient oxygen transfer and protein secretion...
  3. doi request reprint The future of microfluidic assays in drug development
    Yuan Wen
    The Ohio State University, Department of Chemical and Biomolecular Engineering, 140 West 19th Avenue, Columbus, Ohio 43210, USA 1 614 2926611 1 614 2923769
    Expert Opin Drug Discov 3:1237-53. 2008
    ....
  4. ncbi request reprint CELLutions--CHI's Second Annual Summit: The Future of Stem Cell Sciences. Third annual stem cell sources: targeting cell-based regenerative therapies. 20-23 August 2007, Boston, MA, USA
    Shang Tian Yang
    Ohio State University, College of Engineering, 321B Koffolt, 140 W Nineteenth Avenue, Columbus, OH 43210, USA
    IDrugs 10:696-8. 2007
  5. ncbi request reprint Microbioreactors for high-throughput cytotoxicity assays
    Shang Tian Yang
    The Ohio State University, Department of Chemical and Biomolecular Engineering, 140 West 19th Avenue, Columbus, OH 43210, USA
    Curr Opin Drug Discov Devel 11:111-27. 2008
    ..High-density microfluidic arrays provide a platform for future high-throughput and high-content screening that will contribute to drug discovery and bioprocess development...
  6. ncbi request reprint Production of galacto-oligosaccharides from lactose by Aspergillus oryzae beta-galactosidase immobilized on cotton cloth
    Nedim Albayrak
    Department of Chemical Engineering, The Ohio State University, 140 West 19th Avenue, Columbus, Ohio 43210, USA
    Biotechnol Bioeng 77:8-19. 2002
    ..5 and 40 degrees C. The corresponding reactor productivities were 80 and 106 g/L/h, respectively, which are at least several-fold higher than those previously reported...
  7. ncbi request reprint Production of L(+)-lactic acid from glucose and starch by immobilized cells of Rhizopus oryzae in a rotating fibrous bed bioreactor
    Abdullatif Tay
    Department of Chemical Engineering, The Ohio State University 140 West 19th Avenue, Columbus, Ohio 43210, USA
    Biotechnol Bioeng 80:1-12. 2002
    ..The results showed that the oxygen uptake rate increased with increasing DO, indicating that oxygen transfer was limited by the diffusion inside the mycelial layer...
  8. ncbi request reprint Fabrication of well-defined PLGA scaffolds using novel microembossing and carbon dioxide bonding
    Yong Yang
    The Department of Chemical and Biomolecular Engineering, The Ohio State University, 140 West 19th Avenue, Columbus, OH 43210, USA
    Biomaterials 26:2585-94. 2005
    ....
  9. ncbi request reprint Effects of three-dimensional culturing on osteosarcoma cells grown in a fibrous matrix: analyses of cell morphology, cell cycle, and apoptosis
    Chunnuan Chen
    Department of Chemical Engineering, The Ohio State University, 140 West 19th Avenue, Columbus, Ohio 43210, USA
    Biotechnol Prog 19:1574-82. 2003
    ..These results could explain why the fibrous bed bioreactor had good long-term stability and was advantageous for production of non-growth-associated proteins by animal cell cultures...
  10. ncbi request reprint Adaptation of Clostridium tyrobutyricum for enhanced tolerance to butyric acid in a fibrous-bed bioreactor
    Ying Zhu
    Department of Chemical Engineering, The Ohio State University, 140 West 19th Avenue, Columbus, Ohio 43210, USA
    Biotechnol Prog 19:365-72. 2003
    ..This study shows that cell immobilization in the FBB provides an effective means for in-process adaptation and selection of mutant with higher tolerance to inhibitory fermentation product...
  11. doi request reprint Effects of ptb knockout on butyric acid fermentation by Clostridium tyrobutyricum
    Yali Zhang
    William G Lowrie Deptartment of Chemical and Biomolecular Engineering, The Ohio State University, 140 West 19th Avenue, Columbus, OH 43210, USA
    Biotechnol Prog 28:52-9. 2012
    ..This mutant thus can be used more efficiently than the parental strain in fermentations to produce butyrate, acetate, and hydrogen from glucose and xylose...
  12. ncbi request reprint Continuous production of butanol by Clostridium acetobutylicum immobilized in a fibrous bed bioreactor
    Wei Cho Huang
    Department of Chemical Engineering, Ohio State University, 140 West 19th Avenue, Columbus, OH 43210, USA
    Appl Biochem Biotechnol 113:887-98. 2004
    ..9 h-1 and pH 4.3. Compared to the conventional acetone-butanol-ethanol fermentation, the new fermentation process greatly improved butanol yield, making butanol production from corn an attractive alternative to ethanol fermentation...
  13. ncbi request reprint Construction and characterization of ack knock-out mutants of Propionibacterium acidipropionici for enhanced propionic acid fermentation
    Supaporn Suwannakham
    Department of Chemical and Biomolecular Engineering, The Ohio State University, 140 West 19th Avenue, Columbus, Ohio 43210, USA
    Biotechnol Bioeng 94:383-95. 2006
    ..Both mutants produced approximately 14% less acetate from glucose. Although ack disruption alone did not completely eliminate acetate production, the propionate yield was increased by approximately 13%...
  14. ncbi request reprint Construction and characterization of pta gene-deleted mutant of Clostridium tyrobutyricum for enhanced butyric acid fermentation
    Ying Zhu
    Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, Ohio 43210, USA
    Biotechnol Bioeng 90:154-66. 2005
    ..tyrobutyricum in order to improve butyric acid production from glucose...
  15. ncbi request reprint Enhanced propionic acid fermentation by Propionibacterium acidipropionici mutant obtained by adaptation in a fibrous-bed bioreactor
    Supaporn Suwannakham
    Department of Chemical and Biomolecular Engineering, The Ohio State University, 140 West 19th Avenue, Columbus, Ohio 43210, USA
    Biotechnol Bioeng 91:325-37. 2005
    ..acidipropionici was thus attributed to both a high viable cell density maintained in the reactor and favorable mutations resulted from adaptation by cell immobilization in the FBB...
  16. ncbi request reprint Surface modification for enhancing antibody binding on polymer-based microfluidic device for enzyme-linked immunosorbent assay
    Yunling Bai
    Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, Ohio 43210, USA
    Langmuir 22:9458-67. 2006
    ....
  17. ncbi request reprint A fibrous-bed bioreactor for continuous production of developmental endothelial locus-1 by osteosarcoma cells
    Chunnuan Chen
    Department of Chemical Engineering, The Ohio State University, 140 West 19th Avenue, Columbus, OH 43210, USA
    J Biotechnol 97:23-39. 2002
    ..Scanning electron microscopic and confocal scanning laser microscopic studies of the cell-matrix showed that cells formed large aggregates in the fibrous matrix and cell density was relatively uniform in the matrix...
  18. ncbi request reprint Effects of three-dimensional culturing in a fibrous matrix on cell cycle, apoptosis, and MAb production by hybridoma cells
    Jun Luo
    Department of Chemical Engineering, The Ohio State University, 140 West 19th Avenue, Columbus, Ohio 43210, USA
    Biotechnol Prog 20:306-15. 2004
    ....
  19. ncbi request reprint Biotransformation of R-2-hydroxy-4-phenylbutyric acid by D-lactate dehydrogenase and Candida boidinii cells containing formate dehydrogenase coimmobilized in a fibrous bed bioreactor
    Yunling Bai
    Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, 43210, USA
    Biotechnol Bioeng 92:137-46. 2005
    ..h and product yield of 0.95 mol/mol OPBA when the concentrations of OPBA and R-HPBA were less than 10 g/L...
  20. ncbi request reprint Extractive fermentation for butyric acid production from glucose by Clostridium tyrobutyricum
    Zetang Wu
    Department of Chemical Engineering, The Ohio State University, 140 West 19th Avenue, Columbus, Ohio, USA
    Biotechnol Bioeng 82:93-102. 2003
    ..The solvent was found to be toxic to free cells in suspension, but not harmful to cells immobilized in the fibrous bed. The process was stable and provided consistent long-term performance for the entire 2-week period of study...
  21. doi request reprint Effects of different replicons in conjugative plasmids on transformation efficiency, plasmid stability, gene expression and n-butanol biosynthesis in Clostridium tyrobutyricum
    Mingrui Yu
    William G Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 140 West 19th Ave, Columbus, OH 43210, USA
    Appl Microbiol Biotechnol 93:881-9. 2012
    ..A high butanol titer of 20.5 g/L with 0.33 g/g yield and 0.32 g/L h productivity was obtained with mannitol as the substrate in batch fermentation with pH controlled at ~6.0...
  22. doi request reprint Microplate-reader compatible perfusion microbioreactor array for modular tissue culture and cytotoxicity assays
    Yuan Wen
    William G Lowrie Dept of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH 43210, USA
    Biotechnol Prog 26:1135-44. 2010
    ..Compared with conventional 2D cytotoxicity assay, significant drug resistance was observed in the 3D perfusion culture...
  23. doi request reprint Butyric acid production from sugarcane bagasse hydrolysate by Clostridium tyrobutyricum immobilized in a fibrous-bed bioreactor
    Dong Wei
    State Key Laboratory of Pulp and Paper Engineering, School of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, PR China William G Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 140 West 19th Avenue, Columbus, OH 43210, USA
    Bioresour Technol 129:553-60. 2013
    ..9 g/L was obtained in a fed-batch culture, with an overall productivity of 0.51 g/Lh and butyrate yield of 0.48 g/g sugar consumed. This work demonstrated the feasibility of using SCB as a low-cost feedstock to produce butyric acid...
  24. doi request reprint Engineering Propionibacterium acidipropionici for enhanced propionic acid tolerance and fermentation
    An Zhang
    William G Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 140 West 19th Avenue, Columbus, Ohio 43210, USA
    Biotechnol Bioeng 104:766-73. 2009
    ..The adapted mutant obtained from the FBB showed significantly reduced growth sensitivity to propionic acid inhibition, increased H+-ATPase expression and activity, and significantly elongated rod morphology...
  25. ncbi request reprint Astrocyte growth and glial cell line-derived neurotrophic factor secretion in three-dimensional polyethylene terephthalate fibrous matrices
    Shubhayu Basu
    Department of Chemical and Biomolecular Engineering, Ohio State University, Columbus, Ohio 43210, USA
    Tissue Eng 11:940-52. 2005
    ..This work shows promise in culturing astrocytes in PET matrices as the first step in developing a potential implantable tissue-engineering device for treating patients with Parkinson's disease...
  26. ncbi request reprint A hollow-fiber membrane extraction process for recovery and separation of lactic acid from aqueous solution
    Hanjing Huang
    Department of Chemical Engineering, The Ohio State University, 140 West 19th Avenue, Columbus, OH 43210, USA
    Appl Biochem Biotechnol 113:671-88. 2004
    ..A mathematical model considering both diffusion and chemical reaction in the extractor and back extractor was developed to simulate the process. The model fits the experimental data well and can be used in scale up design of the process...
  27. doi request reprint Fed-batch fermentation for n-butanol production from cassava bagasse hydrolysate in a fibrous bed bioreactor with continuous gas stripping
    Congcong Lu
    William G Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 140 W 19th Ave, Columbus, OH 43210, USA
    Bioresour Technol 104:380-7. 2012
    ..8%) and almost no acids, resulting in a highly concentrated butanol solution of ~64% (w/v) after phase separation...
  28. doi request reprint Centrifugal seeding of mammalian cells in nonwoven fibrous matrices
    Robin Ng
    William G Lowrie Dept of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH 43210, USA
    Biotechnol Prog 26:239-45. 2010
    ..The centrifugal seeding method developed in this work can be used to efficiently and uniformly seed small fibrous scaffolds for applications in 3D cell-based assays for high-throughput screening...
  29. doi request reprint High-throughput 3-D cell-based proliferation and cytotoxicity assays for drug screening and bioprocess development
    Xudong Zhang
    William G Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 140 West 19th Avenue, Columbus, OH 43210, USA
    J Biotechnol 151:186-93. 2011
    ..These studies demonstrated that the 3-D culture microplate system with EGFP expressing cells can be used as a high-throughput system in drug discovery and bioprocess development...
  30. ncbi request reprint Production of carboxylic acids from hydrolyzed corn meal by immobilized cell fermentation in a fibrous-bed bioreactor
    Yu Liang Huang
    Department of Chemical Engineering, The Ohio State University, Columbus 43210, USA
    Bioresour Technol 82:51-9. 2002
    ..It is concluded that the FBB technology is suitable for producing value-added biochemicals directly from agricultural residues or commodities such as corn meal...
  31. ncbi request reprint Immobilization of beta-galactosidase on fibrous matrix by polyethyleneimine for production of galacto-oligosaccharides from lactose
    Nedim Albayrak
    Department of Chemical Engineering, The Ohio State University, 140 West 19th Avenue, Columbus 43210, USA
    Biotechnol Prog 18:240-51. 2002
    ..5 and 40 degrees C. The corresponding reactor productivity was 6 kg/L/h, which is several-hundred-fold higher than those previously reported...
  32. doi request reprint High-titer n-butanol production by clostridium acetobutylicum JB200 in fed-batch fermentation with intermittent gas stripping
    Chuang Xue
    Department of Life Science and Biotechnology, Dalian University of Technology, Dalian, China
    Biotechnol Bioeng 109:2746-56. 2012
    ..Compared to conventional ABE fermentation, the fed-batch fermentation with intermittent gas stripping has the potential to reduce at least 90% of energy consumption and water usage in n-butanol production from glucose...
  33. doi request reprint Metabolic engineering of Clostridium tyrobutyricum for n-butanol production
    Mingrui Yu
    William G Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH 43210, USA
    Metab Eng 13:373-82. 2011
    ..0% and 1.5% (v/v) butanol. These results suggest that C. tyrobutyricum is a promising heterologous host for n-butanol production from renewable biomass...
  34. ncbi request reprint Design of a compact disk-like microfluidic platform for enzyme-linked immunosorbent assay
    Siyi Lai
    Department of Chemical Engineering, Ohio State University, 140 West 19th Avenue, Columbus, OH 43210, USA
    Anal Chem 76:1832-7. 2004
    ....
  35. doi request reprint Three-dimensional neural differentiation of embryonic stem cells with ACM induction in microfibrous matrices in bioreactors
    Ning Liu
    William G Lowrie Dept of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH, 43210
    Biotechnol Prog 29:1013-22. 2013
    ..2013 American Institute of Chemical Engineers Biotechnol. Prog., 29:1013-1022, 2013. ..
  36. doi request reprint Metabolic engineering of Rhizopus oryzae: effects of overexpressing pyc and pepc genes on fumaric acid biosynthesis from glucose
    Baohua Zhang
    William G Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 140 West 19th Avenue, Columbus, OH 43210, USA
    Metab Eng 14:512-20. 2012
    ..13-0.36 g/g glucose. This study is the first attempt to use metabolic engineering to modify the fumaric acid biosynthesis pathway to increase fumaric acid production in R. oryzae...
  37. doi request reprint Medium to high throughput screening: microfabrication and chip-based technology
    Yuan Wen
    William G Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, Ohio, USA
    Adv Exp Med Biol 745:181-209. 2012
    ..Emphasis is placed on microscale cell and tissue culture models for medium and high throughput systemic toxicity studies in vitro...
  38. ncbi request reprint Effects of mixing intensity on cell seeding and proliferation in three-dimensional fibrous matrices
    Anli Ouyang
    Department of Chemical and Biomolecular Engineering, The Ohio State University, 140 West 19th Ave Columbus, Ohio 43210, USA
    Biotechnol Bioeng 96:371-80. 2007
    ..Increasing mixing intensity also increased oxygen transfer, decreased the lactate yield from glucose, and improved cell growth...
  39. ncbi request reprint Effect of pH on metabolic pathway shift in fermentation of xylose by Clostridium tyrobutyricum
    Ying Zhu
    Department of Chemical Engineering, The Ohio State University, 140 West 19th Avenue, Columbus, OH 43210, USA
    J Biotechnol 110:143-57. 2004
    ..Difference in the specific metabolic rate of xylose at different pHs suggests that the balance in NADH is a key in controlling the metabolic pathway used by the cells in the fermentation...
  40. doi request reprint Cell surface display of carbonic anhydrase on Escherichia coli using ice nucleation protein for CO₂ sequestration
    Li Hai Fan
    William G Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, Ohio 43210, USA
    Biotechnol Bioeng 108:2853-64. 2011
    ..Cells with surface displayed CA were successfully used to facilitate CO(2) sequestration in contained liquid membrane (CLM)...
  41. doi request reprint Butanol production from wood pulping hydrolysate in an integrated fermentation-gas stripping process
    Congcong Lu
    William G Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 140 W 19th Ave, Columbus, OH 43210, USA
    Bioresour Technol 143:467-75. 2013
    ..73g/L, demonstrating that gas stripping was effective in alleviating butanol toxicity by selectively separating butanol from the fermentation broth, which greatly improved solvents production and sugar conversion in the fermentation. ..
  42. doi request reprint Microfibrous carriers for cell culture: a comparative study
    Yuan Wen
    William G Lowrie Dept of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH 43210, USA
    Biotechnol Prog 27:1126-36. 2011
    ..In addition, the microfibrous carriers are available at a much lower cost than commercial microcarriers, providing an attractive alternative to microcarrier-based large-scale cell cultures...
  43. doi request reprint Beneficial effect of protracted sterilization of lentils on phytase production by Aspergillus ficuum in solid state fermentation
    Patrick Bennett
    William G Lowrie Dept of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH 43210, USA
    Biotechnol Prog 28:1263-70. 2012
    ....
  44. ncbi request reprint Construction and characterization of ack deleted mutant of Clostridium tyrobutyricum for enhanced butyric acid and hydrogen production
    Xiaoguang Liu
    Department of Chemical and Biomolecular Engineering, The Ohio State University, 140 West 19th Avenue, Columbus, Ohio 43210, USA
    Biotechnol Prog 22:1265-75. 2006
    ..43 g/g xylose) and lactate (0.61 g/g xylose) and little butyrate (0.05 g/g xylose), indicating a dramatic metabolic pathway shift caused by the ack deletion in the mutant...
  45. ncbi request reprint A two-stage perfusion fibrous bed bioreactor system for mass production of embryonic stem cells
    Anli Ouyang
    Eli Lilly and Company, Bioprocess Research and Development, Lilly Corporate Center, DC 3945, Indianapolis, IN 46285, USA
    Expert Opin Biol Ther 8:895-909. 2008
    ..Current supplies of ESCs are limited by the available cell sources and inefficient culture methods that grow ESCs on surfaces coated with expensive extracellular matrix (ECM) proteins and in media containing expensive growth factors...