Takao Yagi

Summary

Affiliation: The Scripps Research Institute
Country: USA

Publications

  1. pmc Successful amelioration of mitochondrial optic neuropathy using the yeast NDI1 gene in a rat animal model
    Mathieu Marella
    Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California, United States of America
    PLoS ONE 5:e11472. 2010
  2. pmc No immune responses by the expression of the yeast Ndi1 protein in rats
    Mathieu Marella
    Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California, United States of America
    PLoS ONE 6:e25910. 2011
  3. pmc Protective Role of rAAV-NDI1, Serotype 5, in an Acute MPTP Mouse Parkinson's Model
    Jennifer Barber-Singh
    Department of Molecular and Experimental Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, MEM256, La Jolla, CA 92037, USA
    Parkinsons Dis 2011:438370. 2010
  4. ncbi request reprint NADH dehydrogenases: from basic science to biomedicine
    T Yagi
    Department of Molecular, and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037, USA
    J Bioenerg Biomembr 33:233-42. 2001
  5. ncbi request reprint Can a single subunit yeast NADH dehydrogenase (Ndi1) remedy diseases caused by respiratory complex I defects?
    Takao Yagi
    Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Reseach Institute, La Jolla, California 92037, USA
    Rejuvenation Res 9:191-7. 2006
  6. ncbi request reprint Possibility of transkingdom gene therapy for complex I diseases
    Takao Yagi
    Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA
    Biochim Biophys Acta 1757:708-14. 2006
  7. ncbi request reprint The proton-translocating NADH-quinone oxidoreductase in the respiratory chain: the secret unlocked
    Takao Yagi
    Department of Molecular and Experimental Medicine, MEM 256, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Biochemistry 42:2266-74. 2003
  8. ncbi request reprint Characterization of the membrane domain subunit NuoK (ND4L) of the NADH-quinone oxidoreductase from Escherichia coli
    Mou Chieh Kao
    Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Biochemistry 44:9545-54. 2005
  9. ncbi request reprint Characterization of the membrane domain subunit NuoJ (ND6) of the NADH-quinone oxidoreductase from Escherichia coli by chromosomal DNA manipulation
    Mou Chieh Kao
    Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Biochemistry 44:3562-71. 2005
  10. ncbi request reprint Characterization of the NuoM (ND4) subunit in Escherichia coli NDH-1: conserved charged residues essential for energy-coupled activities
    Jesus Torres-Bacete
    Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 282:36914-22. 2007

Collaborators

Detail Information

Publications52

  1. pmc Successful amelioration of mitochondrial optic neuropathy using the yeast NDI1 gene in a rat animal model
    Mathieu Marella
    Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California, United States of America
    PLoS ONE 5:e11472. 2010
    ..Establishment of animal models of LHON should help elucidate mechanism of the disease and could be utilized for possible development of therapeutic strategies...
  2. pmc No immune responses by the expression of the yeast Ndi1 protein in rats
    Mathieu Marella
    Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California, United States of America
    PLoS ONE 6:e25910. 2011
    ..Long term expression of Ndi1 observed in rodents with no apparent damage to the target tissue was indicative of no action by the host's immune system...
  3. pmc Protective Role of rAAV-NDI1, Serotype 5, in an Acute MPTP Mouse Parkinson's Model
    Jennifer Barber-Singh
    Department of Molecular and Experimental Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, MEM256, La Jolla, CA 92037, USA
    Parkinsons Dis 2011:438370. 2010
    ..It is conceivable that the AAV-serotype5 carrying the NDI1 gene is a powerful tool for proof-of-concept study to demonstrate complex I defects as the causable factor in diseases of the brain...
  4. ncbi request reprint NADH dehydrogenases: from basic science to biomedicine
    T Yagi
    Department of Molecular, and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037, USA
    J Bioenerg Biomembr 33:233-42. 2001
    ..In addition, background information and the significance of various approaches employed to pursue these research objectives will be described...
  5. ncbi request reprint Can a single subunit yeast NADH dehydrogenase (Ndi1) remedy diseases caused by respiratory complex I defects?
    Takao Yagi
    Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Reseach Institute, La Jolla, California 92037, USA
    Rejuvenation Res 9:191-7. 2006
    ..The Ndi1 protein has a great potential as a molecular remedy for complex I deficiencies...
  6. ncbi request reprint Possibility of transkingdom gene therapy for complex I diseases
    Takao Yagi
    Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA
    Biochim Biophys Acta 1757:708-14. 2006
    ..The use of NDI1 as a potential molecular therapy for complex I-deficient diseases is briefly discussed, including the proposed animal model...
  7. ncbi request reprint The proton-translocating NADH-quinone oxidoreductase in the respiratory chain: the secret unlocked
    Takao Yagi
    Department of Molecular and Experimental Medicine, MEM 256, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Biochemistry 42:2266-74. 2003
  8. ncbi request reprint Characterization of the membrane domain subunit NuoK (ND4L) of the NADH-quinone oxidoreductase from Escherichia coli
    Mou Chieh Kao
    Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Biochemistry 44:9545-54. 2005
    ..Possible roles of these arginine residues and other conserved residues in the NuoK subunit for NDH-1 function were discussed...
  9. ncbi request reprint Characterization of the membrane domain subunit NuoJ (ND6) of the NADH-quinone oxidoreductase from Escherichia coli by chromosomal DNA manipulation
    Mou Chieh Kao
    Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Biochemistry 44:3562-71. 2005
    ..Together with the results on mutations related to human diseases, possible functional roles of the NuoJ subunit have been discussed...
  10. ncbi request reprint Characterization of the NuoM (ND4) subunit in Escherichia coli NDH-1: conserved charged residues essential for energy-coupled activities
    Jesus Torres-Bacete
    Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 282:36914-22. 2007
    ..The data suggest that these His are not involved in the catalytic Q-binding. Functional roles of NuoM and advantages of NDH-1 research as a model for mitochondrial complex I study have been discussed...
  11. ncbi request reprint Functional expression of the single subunit NADH dehydrogenase in mitochondria in vivo: a potential therapy for complex I deficiencies
    Byoung Boo Seo
    Division of Biochemistry, Department of Molecular and Experimental Medicine, Scripps Research Institute, La Jolla, CA 92037, USA
    Hum Gene Ther 15:887-95. 2004
    ..The data indicate that the NDI1 gene will be a promising therapeutic tool in the treatment of encephalomyopathies and neurodegenerative diseases caused by complex I impairments...
  12. ncbi request reprint Functional roles of four conserved charged residues in the membrane domain subunit NuoA of the proton-translocating NADH-quinone oxidoreductase from Escherichia coli
    Mou Chieh Kao
    Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 279:32360-6. 2004
    ..The possible functional roles of residues Asp-79 and Glu-81 were discussed...
  13. pmc Features of subunit NuoM (ND4) in Escherichia coli NDH-1: TOPOLOGY AND IMPLICATION OF CONSERVED GLU144 FOR COUPLING SITE 1
    Jesus Torres-Bacete
    Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 284:33062-9. 2009
    ..Only two mutants E144A/F140E and E144A/L147E, one helix turn downstream and upstream restored the energy transducing activities of NDH-1. Based on these results, a role of Glu(144) for proton translocation has been discussed...
  14. pmc Critical roles of subunit NuoH (ND1) in the assembly of peripheral subunits with the membrane domain of Escherichia coli NDH-1
    Prem Kumar Sinha
    Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 284:9814-23. 2009
    ..Implications for the involvement of NuoH (ND1) in maintaining the structure and function of NDH-1 are discussed...
  15. pmc Roles of subunit NuoK (ND4L) in the energy-transducing mechanism of Escherichia coli NDH-1 (NADH:quinone oxidoreductase)
    Jesus Torres-Bacete
    Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 287:42763-72. 2012
    ..The probable roles of subunit NuoK in the energy transducing mechanism of NDH-1 are discussed...
  16. pmc Neuroprotective effect of long-term NDI1 gene expression in a chronic mouse model of Parkinson disorder
    Jennifer Barber-Singh
    The Scripps Research Institute, Department of Molecular and Experimental Medicine, La Jolla, California 92037, USA
    Rejuvenation Res 12:259-67. 2009
    ..The data presented in this study demonstrate a protective effect of the NDI1 gene in dopaminergic neurons, suggesting its therapeutic potential for Parkinson-like disorders...
  17. pmc Pivotal roles of three conserved carboxyl residues of the NuoC (30k) segment in the structural integrity of proton-translocating NADH-quinone oxidoreductase from Escherichia coli
    Norma Castro-Guerrero
    Department of Molecular and Experimental Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, United States
    Biochemistry 49:10072-80. 2010
    ..Together with available information for the hydrophobic subunits, we propose that Glu-138, Glu-140, and Asp-143 of the NuoC segment may have a pivotal role in the structural stability of NDH-1...
  18. pmc Electron transfer in subunit NuoI (TYKY) of Escherichia coli NADH:quinone oxidoreductase (NDH-1)
    Prem Kumar Sinha
    Department of Molecular and Experimental Medicine, MEM 256, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 287:17363-73. 2012
    ..Replacement of Ile-100 and Ile-94 with Ala/Gly did not affect the electron transfer activity significantly. It is concluded that conserved Ile-100 and Ile-94 are not essential for the electron transfer...
  19. ncbi request reprint Roles of bound quinone in the single subunit NADH-quinone oxidoreductase (Ndi1) from Saccharomyces cerevisiae
    Tetsuo Yamashita
    Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 282:6012-20. 2007
    ..The data suggest that Ndi1 bears at least two distinct Q sites: one for bound Q and the other for catalytic Q...
  20. ncbi request reprint Amino acid residues associated with cluster N3 in the NuoF subunit of the proton-translocating NADH-quinone oxidoreductase from Escherichia coli
    Isabel Velazquez
    Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA
    FEBS Lett 579:3164-8. 2005
    ..92, 1.95, and 2.01) in NuoE subunit. These clusters were assigned to clusters N3 and N1a, respectively. Based on the site-directed mutagenesis experiments, we identified that cluster N3 is ligated to the 351Cx2Cx2Cx40C398 motif...
  21. ncbi request reprint In vivo complementation of complex I by the yeast Ndi1 enzyme. Possible application for treatment of Parkinson disease
    Byoung Boo Seo
    Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 281:14250-5. 2006
    ..These results indicate that the expressed Ndi1 protein elicits resistance to MPTP-induced neuronal injury. The present study is the first successful demonstration of complementation of complex I by the Ndi1 enzyme in animals...
  22. pmc Energy transducing roles of antiporter-like subunits in Escherichia coli NDH-1 with main focus on subunit NuoN (ND2)
    Motoaki Sato
    Department of Molecular and Experimental Medicine, MEM 256, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 288:24705-16. 2013
    ..A truncation study indicated that the C-terminal amphipathic segments of NTM14 interacts with the Mβ sheet located on the opposite side of helix HL. Taken together, the mechanism of H(+) translocation in NDH-1 is discussed. ..
  23. ncbi request reprint The single subunit NADH dehydrogenase reduces generation of reactive oxygen species from complex I
    Byoung Boo Seo
    Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    FEBS Lett 580:6105-8. 2006
    ..Furthermore, mitochondria from the NDI1-transduced cells showed a suppressed rate of ROS formation by the complex I inhibitors. We conclude that the Ndi1 enzyme is able to suppress ROS overproduction from defective complex I...
  24. pmc The ND2 subunit is labeled by a photoaffinity analogue of asimicin, a potent complex I inhibitor
    Eiko Nakamaru-Ogiso
    Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA
    FEBS Lett 584:883-8. 2010
    ..The cross-linking was blocked by rotenone. This is the first finding that ND2 was photo-crosslinked with a potent complex I inhibitor, suggesting its involvement in the inhibitor/quinone-binding...
  25. ncbi request reprint Characterization of the iron-sulfur cluster N7 (N1c) in the subunit NuoG of the proton-translocating NADH-quinone oxidoreductase from Escherichia coli
    Eiko Nakamaru-Ogiso
    Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 280:301-7. 2005
    ....
  26. pmc Parkinson's disease and mitochondrial complex I: a perspective on the Ndi1 therapy
    Mathieu Marella
    Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA
    J Bioenerg Biomembr 41:493-7. 2009
    ....
  27. ncbi request reprint Subunit proximity in the H+-translocating NADH-quinone oxidoreductase probed by zero-length cross-linking
    Mou Chieh Kao
    Division of Biochemistry, Department of Molecular and Experimental Medicine, MEM 256, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Biochemistry 43:3750-5. 2004
    ..coli membranes expressing these three subunits. The results were the same as those obtained with Paracoccus membranes. On the basis of the data, subunit arrangements of NDH-1 were discussed...
  28. ncbi request reprint Mechanism of cell death caused by complex I defects in a rat dopaminergic cell line
    Mathieu Marella
    Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 282:24146-56. 2007
    ..Under these conditions, ROS formation by complex I is known to be minimal. Possible use of our cellular model is discussed with regard to development of therapeutic strategies for neurodegenerative diseases caused by complex I defects...
  29. ncbi request reprint Characterization of the membrane domain Nqo11 subunit of the proton-translocating NADH-quinone oxidoreductase of Paracoccus denitrificans
    Mou Chieh Kao
    Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037, USA
    Biochemistry 41:4377-84. 2002
    ..coli membranes have been performed by using cysteine mapping and immunochemical analyses. The data suggest that the Nqo11 subunit has three transmembrane segments and its C-terminus protrudes into the cytoplasmic phase...
  30. pmc Protection by the NDI1 gene against neurodegeneration in a rotenone rat model of Parkinson's disease
    Mathieu Marella
    Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California, United States of America
    PLoS ONE 3:e1433. 2008
    ..The present study shows, for the first time, the powerful neuroprotective effect offered by the Ndi1 enzyme in a rotenone rat model of PD...
  31. pmc Complex I inhibition in the visual pathway induces disorganization of the node of Ranvier
    Mathieu Marella
    Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA
    Neurobiol Dis 58:281-8. 2013
    ..The restoration of the nodal structure was specifically correlated with visual recovery in the treated animal. ..
  32. pmc Mitochondrial complex I activity and NAD+/NADH balance regulate breast cancer progression
    Antonio F Santidrian
    Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037, USA
    J Clin Invest 123:1068-81. 2013
    ....
  33. pmc Structural contribution of C-terminal segments of NuoL (ND5) and NuoM (ND4) subunits of complex I from Escherichia coli
    Jesus Torres-Bacete
    Department of Molecular and Experimental Medicine, MEM 256, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 286:34007-14. 2011
    ..A region of NuoL critical for stability of NDH-1 architecture has been discussed...
  34. ncbi request reprint Amiloride inhibition of the proton-translocating NADH-quinone oxidoreductase of mammals and bacteria
    Eiko Nakamaru-Ogiso
    Department of Molecular and Experimental Medicine, MEM 256, The Scripps Research Institute, 10550 Torrey Pines Road, La Jolla, CA 92037, USA
    FEBS Lett 549:43-6. 2003
    ....
  35. ncbi request reprint A single-subunit NADH-quinone oxidoreductase renders resistance to mammalian nerve cells against complex I inhibition
    Byoung Boo Seo
    Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037, USA
    Mol Ther 6:336-41. 2002
    ..It is conceivable that the NDI1 gene will be a promising tool in the treatment of neurodegenerative conditions caused by complex I inhibition...
  36. ncbi request reprint Characterization and topology of the membrane domain Nqo10 subunit of the proton-translocating NADH-quinone oxidoreductase of Paracoccus denitrificans
    Mou Chieh Kao
    Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Biochemistry 42:4534-43. 2003
    ..From the experimentally determined topology together with computer prediction programs, a topological model for the Nqo10 subunit is proposed...
  37. ncbi request reprint The ND5 subunit was labeled by a photoaffinity analogue of fenpyroximate in bovine mitochondrial complex I
    Eiko Nakamaru-Ogiso
    Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037, USA
    Biochemistry 42:746-54. 2003
    ..These results suggest that the ND5 subunit is involved in construction of the inhibitor- and quinone-binding site(s). Furthermore, it seems likely that the ND5 subunit may participate in H(+)(Na(+)) translocation in coupling site 1...
  38. ncbi request reprint Characterization of the iron-sulfur cluster coordinated by a cysteine cluster motif (CXXCXXXCX27C) in the Nqo3 subunit in the proton-translocating NADH-quinone oxidoreductase (NDH-1) of Thermus thermophilus HB-8
    Eiko Nakamaru-Ogiso
    Department of Molecular and Experimental Medicine, Division of Biochemistry, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 277:1680-8. 2002
    ..Considering the fact that the same motif coordinates only tetranuclear clusters in other enzymes so far known, we propose that the CXXCXXXCX(27)C motif in the Nqo3 subunit most likely ligates the [4Fe-4S] cluster...
  39. pmc Total synthesis of piericidin A1 and B1 and key analogues
    Martin J Schnermann
    Departments of Chemistry and Molecular and Experimental Medicine, and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Am Chem Soc 128:11799-807. 2006
    ....
  40. ncbi request reprint Direct interaction between a membrane domain subunit and a connector subunit in the H(+)-translocating NADH-quinone oxidoreductase
    S Di Bernardo
    MEM-256, Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, 10550 Torrey Pines Rd, La Jolla, CA 92037, USA
    FEBS Lett 508:385-8. 2001
    ..MBS treatment of the E. coli membranes generated the 31 kDa band as in the Paracoccus membranes. These results indicate that Nqo7 interacts with probable N2-binding Nqo6...
  41. ncbi request reprint The proton-translocating NADH-quinone oxidoreductase (NDH-1) of thermophilic bacterium Thermus thermophilus HB-8. Complete DNA sequence of the gene cluster and thermostable properties of the expressed NQO2 subunit
    T Yano
    Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 272:4201-11. 1997
    ..With the advantage of thermostability, the T. thermophilus NDH-1 provides a great model system to investigate the structure-function relationship of the NDH-1 enzyme complexes...
  42. ncbi request reprint Mechanism of toxicity of pesticides acting at complex I: relevance to environmental etiologies of Parkinson's disease
    Todd B Sherer
    Center for Neurodegenerative Disease, Rollins School of Public Health, Atlanta, Georgia 15213, USA
    J Neurochem 100:1469-79. 2007
    ....
  43. ncbi request reprint Intersecting pathways to neurodegeneration in Parkinson's disease: effects of the pesticide rotenone on DJ-1, alpha-synuclein, and the ubiquitin-proteasome system
    Ranjita Betarbet
    Center for Neurodegenerative Disease, Center for Neurodegenerative Diseases, Whitehead Biomedical Research Building, 615 Michael Street, Atlanta, GA 30322, USA
    Neurobiol Dis 22:404-20. 2006
    ....
  44. ncbi request reprint Genetic and functional analysis of mitochondrial DNA-encoded complex I genes
    Yidong Bai
    Department of Cellular and Structural Biology, University of Texas Health Science Center at San Antonio, San Antonio, Texas 78229, USA
    Ann N Y Acad Sci 1011:272-83. 2004
    ..In particular, in these transfected cells, the yeast enzyme becomes integrated into the human respiratory chain and fully restores the capacity of the cells to grow in galactose medium...
  45. ncbi request reprint Obligatory role for complex I inhibition in the dopaminergic neurotoxicity of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)
    Jason R Richardson
    Department of Environmental and Occupational Medicine, University of Medicine and Dentistry, New Jersey Robert Wood Johnson Medical School and Environmental and Occupational Health Sciences Institute, Piscataway, New Jersey 08854, USA
    Toxicol Sci 95:196-204. 2007
    ..Furthermore, combined with reports of a complex I defect in Parkinson's disease (PD) patients, the present study affirms the utility of MPTP in understanding the molecular mechanisms underlying dopaminergic neurodegeneration in PD...
  46. ncbi request reprint Characterization of cluster N5 as a fast-relaxing [4Fe-4S] cluster in the Nqo3 subunit of the proton-translocating NADH-ubiquinone oxidoreductase from Paracoccus denitrificans
    Takahiro Yano
    Johnson Research Foundation, Department of Biochemistry and Biophysics, School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    J Biol Chem 278:15514-22. 2003
    ..Hence, we propose that the His-coordinated [4Fe-4S] cluster corresponds to cluster N5...
  47. ncbi request reprint Mechanism of toxicity in rotenone models of Parkinson's disease
    Todd B Sherer
    Center for Neurodegenerative Disease, Emory University, Atlanta, Georgia 30322, USA
    J Neurosci 23:10756-64. 2003
    ..These results, using three models of increasing complexity, demonstrate the involvement of oxidative damage in rotenone toxicity and support the evaluation of antioxidant therapies for Parkinson's disease...
  48. pmc Iron-sulfur cluster N5 is coordinated by an HXXXCXXCXXXXXC motif in the NuoG subunit of Escherichia coli NADH:quinone oxidoreductase (complex I)
    Eiko Nakamaru-Ogiso
    Department of Biochemistry and Biophysics, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Biol Chem 283:25979-87. 2008
    ..These data confirmed that, contrary to the misassignment claim, cluster N5 has a unique coordination with His(Cys)(3) ligands in NuoG...
  49. ncbi request reprint The ND1 subunit constructs the inhibitor binding domain in bovine heart mitochondrial complex I
    Masatoshi Murai
    Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo ku, Kyoto 606 8502, Japan
    Biochemistry 46:6409-16. 2007
    ..Further, the present study corroborates our previous proposition that the inhibition site of Deltalac-acetogenins differs from that of traditional inhibitors...
  50. ncbi request reprint Assembly of respiratory complexes I, III, and IV into NADH oxidase supercomplex stabilizes complex I in Paracoccus denitrificans
    Anke Stroh
    Zentrum der Biologischen Chemie, Universitatsklinikum Frankfurt, D 60590 Frankfurt, Germany
    J Biol Chem 279:5000-7. 2004
    ..In addition to substrate channeling, structural stabilization of a membrane protein complex thus appears as one of the major functions of respiratory chain supercomplexes...
  51. ncbi request reprint Annonacin, a natural mitochondrial complex I inhibitor, causes tau pathology in cultured neurons
    Myriam Escobar-Khondiker
    Experimental Neurology, Philipps University, D 35033 Marburg, Germany
    J Neurosci 27:7827-37. 2007
    ....
  52. ncbi request reprint Redox properties of the [2Fe-2S] center in the 24 kDa (NQO2) subunit of NADH:ubiquinone oxidoreductase (complex I)
    Yanbing Zu
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 2XY, U K
    Biochemistry 41:10056-69. 2002
    ..Finally, the validity of using the [2Fe-2S] cluster in an isolated subunit, as a mechanistic basis for coupled proton-electron transfer in intact complex I, is evaluated...

Research Grants36

  1. MOLECULAR REMEDY OF MITOCHONDRIAL DEFECTS
    Takao Yagi; Fiscal Year: 2002
    ..3) Construction of transgenic mice containing the ND11 gene. (4) Repair of a point mutation in the NDUFA1 gene encoding the MWFE subunit of complex I. ..
  2. NADH-UBIQUINONE REDUCTASE OF PARACOCCUS DENITRIFICANS
    Takao Yagi; Fiscal Year: 2003
    ..iv) Determination of the functional role of the intrinsic membrane segment of bovine heart complex I. (v) Determination of the effects of various culture conditions on the biosynthesis of the Paracoccus NDH-1 complex. ..
  3. NADH-UBIQUINONE REDUCTASE OF PARACOCCUS DENITRIFICANS
    Takao Yagi; Fiscal Year: 2001
    ..iv) Determination of the functional role of the intrinsic membrane segment of bovine heart complex I. (v) Determination of the effects of various culture conditions on the biosynthesis of the Paracoccus NDH-1 complex. ..
  4. MOLECULAR REMEDY OF MITOCHONDRIAL DEFECTS
    Takao Yagi; Fiscal Year: 2007
    ..2) Construction of animal models for human diseases caused by complex I defects. (3) Protection by the Ndi1 enzyme against tissue degradation caused by complex I deficiencies. ..
  5. MOLECULAR REMEDY OF MITOCHONDRIAL DEFECTS
    Takao Yagi; Fiscal Year: 2005
    ..2) Construction of animal models for human diseases caused by complex I defects. (3) Protection by the Ndi1 enzyme against tissue degradation caused by complex I deficiencies. ..
  6. NADH-UBIQUINONE REDUCTASE OF PARACOCCUS DENITRIFICANS
    Takao Yagi; Fiscal Year: 2011
    ..In order to develop therapies for diseases caused by deficiencies of this enzyme, it is a prerequisite to investigate why and how defects occur. ..
  7. NADH-UBIQUINONE REDUCTASE OF PARACOCCUS DENITRIFICANS
    Takao Yagi; Fiscal Year: 2005
    ..3) Determination of subunit-subunit interaction of the NDH-1. (4) Identification of the subunit(s) and the amino acid residue(s) labeled by photoaffinity analogues of inhibitors specific to the NDH-1/complex I. ..
  8. MOLECULAR REMEDY OF MITOCHONDRIAL DEFECTS
    Takao Yagi; Fiscal Year: 2006
    ..2) Construction of animal models for human diseases caused by complex I defects. (3) Protection by the Ndi1 enzyme against tissue degradation caused by complex I deficiencies. ..
  9. NADH-UBIQUINONE REDUCTASE OF PARACOCCUS DENITRIFICANS
    Takao Yagi; Fiscal Year: 2007
    ..3) Determination of subunit-subunit interaction of the NDH-1. (4) Identification of the subunit(s) and the amino acid residue(s) labeled by photoaffinity analogues of inhibitors specific to the NDH-1/complex I. ..
  10. NADH-UBIQUINONE REDUCTASE OF PARACOCCUS DENITRIFICANS
    Takao Yagi; Fiscal Year: 2009
    ..In order to develop therapies for diseases caused by deficiencies of this enzyme, it is a prerequisite to investigate why and how defects occur. ..
  11. MOLECULAR REMEDY OF MITOCHONDRIAL DEFECTS
    Takao Yagi; Fiscal Year: 2009
    ..2) Construction of animal models for human diseases caused by complex I defects. (3) Protection by the Ndi1 enzyme against tissue degradation caused by complex I deficiencies. ..
  12. NADH-UBIQUINONE REDUCTASE OF PARACOCCUS DENITRIFICANS
    Takao Yagi; Fiscal Year: 2010
    ..In order to develop therapies for diseases caused by deficiencies of this enzyme, it is a prerequisite to investigate why and how defects occur. ..
  13. NADH-UBIQUINONE REDUCTASE OF PARACOCCUS DENITRIFICANS
    Takao Yagi; Fiscal Year: 2002
    ..iv) Determination of the functional role of the intrinsic membrane segment of bovine heart complex I. (v) Determination of the effects of various culture conditions on the biosynthesis of the Paracoccus NDH-1 complex. ..
  14. MOLECULAR REMEDY OF MITOCHONDRIAL DEFECTS
    Takao Yagi; Fiscal Year: 1999
    ..The overall goal of this grant application is to find a remedy for the diseases that result from dysfunction of complex I. ..
  15. NADH-UBIQUINONE REDUCTASE OF PARACOCCUS DENITRIFICANS
    Takao Yagi; Fiscal Year: 1993
    ..iii) Construction and characterization of NADH-binding subunit gene deletion mutants for the Paracoccus NADH dehydrogenase complex using gene replacement techniques...
  16. NADH-UBIQUINONE REDUCTASE OF PARACOCCUS DENITRIFICANS
    Takao Yagi; Fiscal Year: 2000
    ..iv) Determination of the functional role of the intrinsic membrane segment of bovine heart complex I. (v) Determination of the effects of various culture conditions on the biosynthesis of the Paracoccus NDH-1 complex. ..
  17. MOLECULAR REMEDY OF MITOCHONDRIAL DEFECTS
    Takao Yagi; Fiscal Year: 2000
    ..3) Construction of transgenic mice containing the ND11 gene. (4) Repair of a point mutation in the NDUFA1 gene encoding the MWFE subunit of complex I. ..
  18. MOLECULAR REMEDY OF MITOCHONDRIAL DEFECTS
    Takao Yagi; Fiscal Year: 2001
    ..3) Construction of transgenic mice containing the ND11 gene. (4) Repair of a point mutation in the NDUFA1 gene encoding the MWFE subunit of complex I. ..
  19. NADH-UBIQUINONE REDUCTASE OF PARACOCCUS DENITRIFICANS
    Takao Yagi; Fiscal Year: 1999
    ..Function roles of the URF products will be clarified by using gene deletion techniques. (v) Studies on effects of various culture conditions on the biosynthesis of the Paracoccus NDH-1 will be performed. ..
  20. Development of therapies to retard Parkinson's disease
    Takao Yagi; Fiscal Year: 2007
    ..2) Suppression of PD's disease like symptoms in rodent models by the Ndi1 expression. ..
  21. NADH-UBIQUINONE REDUCTASE OF PARACOCCUS DENITRIFICANS
    Takao Yagi; Fiscal Year: 1992
    ..iii) Construction and characterization of NADH-binding subunit gene deletion mutants for the Paracoccus NADH dehydrogenase complex using gene replacement techniques...
  22. Development of therapies to retard Parkinson's disease
    Takao Yagi; Fiscal Year: 2006
    ..2) Suppression of PD's disease like symptoms in rodent models by the Ndi1 expression. ..
  23. NADH-UBIQUINONE REDUCTASE OF PARACOCCUS DENITRIFICANS
    Takao Yagi; Fiscal Year: 2006
    ..3) Determination of subunit-subunit interaction of the NDH-1. (4) Identification of the subunit(s) and the amino acid residue(s) labeled by photoaffinity analogues of inhibitors specific to the NDH-1/complex I. ..
  24. MOLECULAR REMEDY OF MITOCHONDRIAL DEFECTS
    Takao Yagi; Fiscal Year: 2004
    ..3) Construction of transgenic mice containing the ND11 gene. (4) Repair of a point mutation in the NDUFA1 gene encoding the MWFE subunit of complex I. ..
  25. NADH-UBIQUINONE REDUCTASE OF PARACOCCUS DENITRIFICANS
    Takao Yagi; Fiscal Year: 2004
    ..3) Determination of subunit-subunit interaction of the NDH-1. (4) Identification of the subunit(s) and the amino acid residue(s) labeled by photoaffinity analogues of inhibitors specific to the NDH-1/complex I. ..
  26. Development of therapies to retard Parkinson's disease
    Takao Yagi; Fiscal Year: 2003
    ..2) Effects of MPTP on transgenic mice expressing the Ndil enzyme in the dopamine-producing tissues. ..
  27. MOLECULAR REMEDY OF MITOCHONDRIAL DEFECTS
    Takao Yagi; Fiscal Year: 2003
    ..3) Construction of transgenic mice containing the ND11 gene. (4) Repair of a point mutation in the NDUFA1 gene encoding the MWFE subunit of complex I. ..